CN104522450A - Corn oligopeptide pollen and preparation method thereof - Google Patents
Corn oligopeptide pollen and preparation method thereof Download PDFInfo
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- CN104522450A CN104522450A CN201410829860.6A CN201410829860A CN104522450A CN 104522450 A CN104522450 A CN 104522450A CN 201410829860 A CN201410829860 A CN 201410829860A CN 104522450 A CN104522450 A CN 104522450A
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Abstract
The invention discloses corn oligopeptide pollen and a preparation method thereof and belongs to the technical field of functional foods. The preparation method comprises the following steps: taking corn bee pollen as a main raw material, and performing low-temperature wall disruption and extraction technology to prepare a corn bee pollen extract; scientifically compounding corn oligopeptide powder, modified dietary fibers, fruit-vegetable powder, pectin hydrolyzate, chrysanthemum powder, sophora flower powder and isomaltitol, thereby obtaining the corn oligopeptide pollen which is strong in corn flavor and proper in taste and can achieve the effects of enhancing the human body immunity and protecting the liver. The effect refers to a result of a synergistic effect of the various components, the various raw material components are scientifically compounded, the generated effect far exceeds the superposed functions and effects of each single component, and the corn oligopeptide pollen has high advancement and practicality. The preparation method is simple in process, convenient to operate and low in equipment requirement, large-scale industrial production can be realized, the modified dietary fibers are finally mixed, and a strong burying effect is achieved, so that the characters of the product are stable, and the quality guarantee period is long.
Description
Technical field
The present invention relates to functional food, particularly a kind of maize oligopeptide pollen and preparation method thereof.
Background technology
The pollen load taken back when Bee Pollen refers to honeybee producting honey, at the pollen formed after storage and fermentation in honeycomb.Honeybee is when gathering honey, and corbiculate leg can collect pollen, and form pollen load, after entering honeycomb, pollen load can be in store.Its main dietotherapy composition is: protein, amino acid, vitamin, Bee Pollen element, trace element, organized enzyme, flavone compound, lipid, nucleic acid, brassin, phytic acid etc.Wherein amino acid content and ratio are the amino acid patterns recommended closest to FAO (Food and Agriculture Organization of the United Nation) (FAO), and this is extremely rare in wholefood.Bee Pollen is the concentrate that the nutritious material be worth with drug effect forms, and it is containing protein, carbohydrate, mineral matter, vitamin and other active material.Bee Pollen is fabulous natural nutrient food, is also a kind of desirable invigorant simultaneously, and has certain medical function.
Bee Pollen derives from the Nature, the pollen grain that to be honeybee gather in phanerogam (nectariferous plant and plant of pollen) pistil, and add special glandular secretion thing (nectar and saliva) and mixed a kind of irregular discoid shape thing.Bee Pollen has unique natural health effect and medical treatment and its cosmetic values, be familiar with by increasing people, it is kind of a high protein and low fat nutritional health food, being described as " all-round nutraceutical " " concentrated natural Drug Storage " " cosmetics for oral administration " etc., is the rarity in mankind's wholefood.
Compared with other natural products, the structure comparison of pollen is special.Pollen grain be by pollen wall, germinal aperature and plasm three part form.Pollen wall, also known as sporoderm, can be divided into inner and outer wall, and inwall is the innermost one deck of sporoderm, smooth, thin and flexible.Outer wall is thicker, hard and lack flexibility, and be one deck that in sporoderm, structure is more complicated, main component is sporopollenin, and this material has the resistance effect of physics, chemistry and enzyme, acidproof, alkaline-resisting, heatproof, withstand voltage, to hydrochloric acid in gastric juice and other digestive system enzyme also highly stable.Research proves: the nutriment in non-broken pollen is not easily digested, and broken wall can make the active material of pollen fully discharge, thus effect of its pollen product and health care are all increased substantially.Pollen wall both have impact on absorbing of pollens nutrition composition, also prevented the release of nutriment during extraction, greatly limit deep development and the utilization of pollen, and therefore, the broken wall of Bee Pollen is also very important.
In various plant pollen, zasiokaurin has obvious predominance. by showing the mensuration of zasiokaurin, containing 18 seed amino acids in zasiokaurin, free aminoacid content that can be directly absorbed by the body is up to 23%, its protein content is 22% ~ 25%, also containing sugar and organic acid etc. zasiokaurin owing to containing higher corn polysaccharide, thus makes it become the kind improving immunologic function best results, has effect that is anti-ageing, Tumor suppression.Lot of experiments research shows, zasiokaurin can promote the growth of spleen, bone, lymph node and thymus gland immune organ, strengthens the activity of immunocyte, improves the ability of body fight bacterium and virus; And have radioresistance, antitumaous effect.Zasiokaurin can provide the multiple nutrients composition of needed by human body simultaneously, for the disease because lacking caused by certain nutrition, also has good auxiliary curative effect.Therefore zasiokaurin is widely used clinically, and as the supplementary therapy product of various diseases and the complementary goods of daily nutrition.
The mixture of the multiple little peptide that corn oligopeptide powder obtains through enzyme degraded as zein, except the good characteristic with peptide matters---be better than the direct absorption of amino acid or protein, dissolubility is strong (all can be completely water-soluble under large-scale pH value, produce without muddy and sediment), stability is by force (to thermally-stabilised, component does not change, function is not lost), high (the wholefood albumen of security, safe and reliable, have no side effect) etc. beyond characteristic, also there is oneself exclusive specific function.Maize oligopeptide (corn peptide) has reliable security.Corn peptide has multiple biologically active, as anti-oxidant, anti-hypertension, develop immunitypty, antifatigue, protection liver etc.It should be noted that the test of animal and human's body finds, corn peptide also has functional characteristic a---sobering-up functions that is important and don't that be same as other food oligopeptide.Thus protection liver.
The health products or the functional food that relate to Bee Pollen in prior art are more, the product of relevant bee pollen of maize is also more, but be all with single broken wall or not broker wall bee pollen sale, functional single, mouthfeel is not bery desirable, Chinese patent CN1330255C discloses a kind of composite melissa powder and production technology, its technical characteristics is: Flos Camelliae Japonicae pollen, Flos Nelumbinis pollen, bee pollen of maize, aloe is extracted as, pearl powder, isoflavones and auxiliary material silica, calcium stearate etc., have and delay senility, make the appearance that women keeps beautiful and young, and also have the effect of health therapy.Chinese patent CN 101731630 B disclose a kind of have relieve the effect of alcohol, protect liver function effect health food, this health food comprises following component by weight percentage: corn oligopeptide powder 1.0% ~ 100.0%; Taurine 0.0% ~ 40.0%; Alanine 0.0% ~ 30.0%; Cysteine 0.0% ~ 50.0%; Leucine 0.0% ~ 30.0%; Glutathione 0.0% ~ 30.0%; Raisin tree seed extract 0.0% ~ 30.0%; Brown rice germ 0.0% ~ 30.0%; Kudzu root extract 0.0% ~ 30.0%; Auxiliary material 0.0% ~ 95.0%.The present invention, by scientific composition, by reasonably combined for the raw material of each exact efficacy, can effectively promote human body alcohol metabolism, reduces alcohol to the toxicity of liver, thus plays the effect of liver of relieving the effect of alcohol, protect.
At present, yet there are no simultaneously with bee pollen of maize and corn oligopeptide powder for raw material and the functional food prepared or health products, and the active principle such as protein, corn polysaccharide in bee pollen of maize is underutilized.
Summary of the invention
Technical problem solved by the invention take bee pollen of maize as primary raw material, bee pollen of maize extract is obtained through low temperature broken wall, extractive technique, then the composite corn oligopeptide powder of science, modified dietary fiber, fruit vegetable powder, pectin decomposer, chrysanthemum powder, sophora flower powder, isomalt, prepares that a kind of corn fragrance is dense, mouthfeel is suitable for, significantly can strengthen the maize oligopeptide pollen of body immunity, liver protecting.
In order to achieve the above object, the present invention is by the following technical solutions:
A kind of maize oligopeptide pollen, prepared by the raw material of following parts by weight:
Bee pollen of maize extract 75-95 part, corn oligopeptide powder 10-30 part, modified dietary fiber 10-20 part, fruit vegetable powder 10-20 part, pectin decomposer 5-20 part, chrysanthemum powder 3-12 part, sophora flower powder 2-10 part, isomalt 2-10 part;
Preferably, described maize oligopeptide pollen, prepared by the raw material of following parts by weight:
Bee pollen of maize extract 80-90 part, corn oligopeptide powder 15-25 part, modified dietary fiber 13-17 part, fruit vegetable powder 14-18 part, pectin decomposer 10-15 part, chrysanthemum powder 8-10 part, sophora flower powder 4-8 part, isomalt 4-8 part;
More preferably, described maize oligopeptide pollen, prepared by the raw material of following parts by weight:
Bee pollen of maize extract 85 parts, corn oligopeptide powder 20 parts, modified dietary fiber 15 parts, fruit vegetable powder 16 parts, pectin decomposer 12 parts, chrysanthemum powder 9 parts, 6 parts, sophora flower powder, isomalt 6 parts;
Further, described bee pollen of maize extract is for raw material with bee pollen of maize, waxy corn palpus, defatted corn powder mixture, obtained through ultrasonic cleaning, high-pressure pulse electric and ultrasonic process, biological enzymolysis and fermentation by saccharomyces cerevisiae successively, achieve: 1) ultrasonic cleaning effectively can remove the residues of pesticides of bee pollen of maize, waxy corn palpus, improves the foodsafety of product, 2) high-pressure pulse electric process, has effectively killed the worm's ovum in bee pollen of maize and waxy corn palpus and miscellaneous bacteria, for the enzymolysis of postorder and sweat normally carry out provide good gnotobasis, 3) omnidistance broken wall, extraction (high-pressure pulse electric, ultrasonic, enzymolysis, culture propagation), improve material effective component recovery rate and nutrient composition content, 4) macro-nutrients (protein, starch, fat etc.) both can be converted into Small molecular and easily absorb nutriment by biological enzymolysis, can realize again the broken wall to bee pollen of maize further, 5) the zymotic fluid nutriment composition kind after fermentation by saccharomyces cerevisiae is more, content is higher, be more easily absorbed and utilized by the body, the alcohol of the propagation but also generation that not only achieve probio (saccharomyces cerevisiae) can increase the stripping of alcohol soluble active principals, improves the content of active ingredient, 6) omnidistance employing low temperature extractive technique, effectively prevent the loss of reproducibility active ingredient and volatile fragrant components (in waxy corn palpus), through water extraction and alcohol extracting, the bee pollen of maize of extract not only containing high level and the active ingredient of waxy corn palpus, and containing the probio saccharomyces cerevisiae useful to human body, effectively can promote that human body is to the absorption of nutriment, improve human body integral immunity, also has strong natural corn fragrance simultaneously, significantly improve the corn fragrance of material effective component content and finished product, enhance appetite, improve the uptake ratio of nutritional labeling, digestibility and absorptivity,
Preferably, the preparation method of described bee pollen of maize extract comprises the steps: that 60-80:3-8:2-6 takes bee pollen of maize respectively in mass ratio, waxy corn palpus and defatted corn powder, first by bee pollen of maize, waxy corn must be put in the supersonic wave cleaning machine that 0.3-0.5% sodium bicarbonate solution is housed respectively, in 200W under room temperature, 40KHz cleans 3-5min, drain, then mix with defatted corn powder, add former bee pollen of maize quality 3-5 water doubly, be 4-6 by lactic acid adjust ph, in electric-field intensity 20-40kV/cm, burst length 400-500 μ s, high-pressure pulse electric process is carried out under pulse frequency 200-300Hz condition, then room temperature 400W, 40KHz condition ultrasonic process 10-15min, be warming up to 40-50 DEG C, add the compound enzyme of former bee pollen of maize quality 2-8%, stir, enzymolysis 15-20min, Temperature fall to 28-35 DEG C, with 3 × 10
5-5 × 10
5cFU/L enzymolysis liquid inoculum concentration inoculation Active Dry Yeast, fermentation 20-48h, fermentation liquor 100-300 eye mesh screen filters, and namely filtrate obtain bee pollen of maize extract through ultrafiltration, reduced pressure concentration, freeze drying,
In described bee pollen of maize extract, active saccharomyces cerevisiae content is 6 × 10
10-9 × 10
10cFU/g;
The quality group of described compound enzyme becomes: protease: carbohydrase: pectase: cellulase: lipase=10-15:5-10:4-6:3-5:1-3.
Further, dietary fiber obtains through physics, chemistry or biological method process by described modified dietary fiber, Soluble Fiber cellulose content is high, biologically active strong, have cellulose that is important, positive role to beneficial bacteria of intestinal tract group, compared with full diet fiber, its biological action is more powerful: 1) Soluble Fiber cellulose content is high, more easily utilized by probio, probio can be improved in the growth of enteron aisle and fertility, increase kind and the quantity of probio flora, reduce large intestine pH value, improve intestine microenvironment; 2) powerful adsorption capacity, through modified, cellulosic specific area increases, and network is enriched, and absorption affinity strengthens, and the organic molecule ability of chelating, absorption cholesterol and bile acids is stronger, suppress human body to their absorption; 3) ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is stronger; 4) the stronger and impact of acid and alkali, alkali, salt of retentiveness, dilatancy, thickening property, 5) resident time of adjustment and maintenance gut flora, strengthen the absorption and digestion ability of enteron aisle, improve body immunity, finally significantly improve the absorption and digestion rate of maize oligopeptide pollen active ingredient; 6) promote gastrointestinal peristalsis, slow down and eliminate the bad reaction such as gasteremphraxis, abdominal distension; 7) bury function is strong, prevents external environment (oxygen, temperature, illumination, humidity etc.) factor from, on the impact of product quality, stabilizing the biologically active of product, extending the shelf-life of product;
Preferably, described modified fibre is obtained through biology enzyme enzymolysis by one or more in inulin, apple fiber, common oats fibre, Semen Tritici aestivi fiber;
More preferably, the preparation method of described modified dietary fiber comprises the following steps: by inulin, apple fiber, common oats fibre, Semen Tritici aestivi fiber 6-11:4-6:3-5:2-4 Homogeneous phase mixing in mass ratio, add its quality 3-7 water doubly, room temperature 200-300W, 35-40KHz condition ultrasonic extraction 10-15min, then at electric-field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric extraction under pulse frequency 200-300Hz condition; Be 4.5-6.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains modified dietary fiber;
Described biology enzyme is zytase, cellulase, laccase, pectase, tannase, amylase and protease 5-9:2-5:2-4:2-4:2-4:1-3:0.2-0.4 Homogeneous phase mixing in mass ratio.
Described pectin decomposer has natural, soft agreeable to the taste tart flavour and significant antagonistic property, especially the strongest to Escherichia coli inhibitory action, also there is stronger non-oxidizability simultaneously, concentration can act on when reaching 0.01%, very strong eliminating effect is all shown to superoxide radical, hydroxy radical and DPPH free radical, when concentration reaches 1.0%, eradicating efficacy reaches 100%;
The science of described pectin decomposer is composite, effectively can improve the palatability of maize oligopeptide pollen, most importantly its significant antagonistic property and non-oxidizability avoid oxidation and the microorganism encroach of maize oligopeptide pollen active ingredient, stabilize and increase the biologically active of maize oligopeptide pollen, extend the shelf-life of product;
Further, described pectin decomposer obtains pectin with waste residues such as the pericarps such as lemon, orange, shaddock, citrus, grape or hawthorn, beet, apples through ultrasonic cleaning, high-voltage pulse extraction, hot water extraction, alcohol precipitation, then obtained through pectinase enzymatic hydrolysis;
Preferably, the preparation method of described pectin decomposer, fresh hawthorn pomace, Grape Skin, pomace, megasse is comprised the steps: to put into the supersonic wave cleaning machine that 1.2% sodium bicarbonate solution is housed respectively and clean 5-10min in 200W, 40KHz, drain, be cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, 6-9:5-8:2-4:2-4 Homogeneous phase mixing in mass ratio, then in-21--25 DEG C of freezing 10-30min, pulverize immediately, particle diameter is 0.1-1mm, add crushed material quality 3-5 deionized water doubly, mix, be 2-3 by citric acid adjust ph, at electric-field intensity 35-45kV/cm under room temperature, burst length 400-600 μ s, high-pressure pulse electric process is carried out under pulse frequency 200-300Hz condition, then normal pressure boils 30-50min, Bag filter, filter residue its quality 1-3 filtrate normal pressure doubly boils 40-60min, Bag filter, merging filtrate, diatomite filtration, filtrate is clarified, at 75 DEG C, be evaporated to solid content is 6-10%, be cooled to room temperature rapidly, the pH value adding concentrate 1.5 times of volumes is 95% ethanol of 2-3, leave standstill 20-30min, pectin is precipitated out, the centrifugal 5-10min of 4000r/min, reclaim precipitation, with the absolute ethyl alcohol wash-out of 2 times of volumes, centrifugal, reclaim precipitation, operation like this 2 times, what obtain is deposited in 40-60 DEG C of vacuum drying 2-3h, obtain pectin, add pectin quality 4-5 40-50 DEG C of water doubly, stir, Homogeneous phase mixing, insulation, be 4.5-5.5 by lactic acid adjust ph, add the pectinase enzymatic hydrolysis 2-4h of 0.3-0.8U/mL, enzymolysis liquid filters, filtrate is gone out enzyme 10-15min in boiling water, be cooled to room temperature, ultrafiltration concentration, namely freeze drying obtains pectin decomposer.
Further, described fruit vegetable powder is one or more Homogeneous phase mixing in acerola concentrate powder, lemon powder, blueberry powder, blackberry powder, Cranberry powder, raspberry powder, papaya powder, mango powder, leek powder, white radish powder, white gourd powder, mung bean sprouts powder, carrot meal, celery powder, pineapple powder, orange powder;
Preferably, the mass percent of described fruit vegetable powder consists of: acerola concentrate powder 15%, lemon powder 15%, blueberry powder 12%, blackberry powder 10%, Cranberry powder 8%, raspberry powder 8%, papaya powder 5%, mango powder 5%, leek powder 5%, white radish powder 3%, white gourd powder 3%, mung bean sprouts powder 3%, carrot meal 2%, celery powder 2%, pineapple powder 2%, orange powder 2%;
Preferably, the preparation method of each fruit vegetable powder described is: put by fruits and vegetables in the supersonic wave cleaning machine that 1.2% sodium bicarbonate solution is housed and clean 10-15min in 200W, 40KHz, drain, be cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, put in microwave dryer and carry out microwave drying 5-25min under power 3000W, frequency 2450MHz, temperature 50-60 DEG C condition, until moisture is down to 5-8%, then be crushed to particle diameter 0.1-0.3mm in 20-30 DEG C, pack and obtain fruit vegetable powder.
Further, described chrysanthemum powder or sophora flower powder adopt the low temperature extraction process such as ultrasonic cleaning, microwave drying and biological enzymolysis and obtained, the active component of chrysanthemum or sophora flower can be retained to greatest extent, keep chrysanthemum or the natural color and luster of sophora flower and fragrance, its nutritional labeling kind is complete, content is high, molecular weight is little, is easy to human consumption and absorption;
Preferably, the preparation method of described chrysanthemum powder or sophora flower powder is: by the fresh chrysanthemum of harvesting or sophora flower impurity elimination, put into 0.6-0.8% sodium bicarbonate solution is housed supersonic wave cleaning machine in 200W, 40KHz cleans 10-15min, drain, put in microwave dryer in power 3000W, frequency 2450MHz, microwave drying 5-15min is carried out under temperature 50-60 DEG C of condition, until moisture is down to 8-10%, then be crushed to particle diameter 0.1-0.3mm in 20-30 DEG C and must pulverize pollen, add and pulverize pollen quality 1-3 water doubly, be 4-6 by lactic acid adjust ph, in electric-field intensity 20-40kV/cm, burst length 400-500 μ s, high-pressure pulse electric process is carried out under pulse frequency 200-300Hz condition, then room temperature 400W, 40KHz condition ultrasonic process 10-15min, be warming up to 40-50 DEG C, add the mixed enzyme pulverizing pollen quality 3-5%, stir, enzymolysis 15-20min, enzymolysis liquid filters through 100-300 eye mesh screen, and namely filtrate obtain chrysanthemum powder or sophora flower powder through ultrafiltration, reduced pressure concentration, freeze drying,
The quality group of described mixed enzyme becomes: protease: pectase: cellulase: lipase=10-15:5-10:4-6:3-5.
The preparation method of described maize oligopeptide pollen, comprise the steps: according to formula rate, accurately take each raw material, successively isomalt, sophora flower powder, chrysanthemum powder, pectin decomposer, fruit vegetable powder, corn oligopeptide powder, bee pollen of maize extract are added V-type blending tank Homogeneous phase mixing 20-40min, speed of agitator 20-40r/min, then modified dietary fiber Homogeneous phase mixing 10-20min is added, speed of agitator 40-60r/min, cross 60 mesh sieves, sterile filling, sealing, packaging get product maize oligopeptide pollen;
Preferably, described sterile filling adopts automatic powdery bottle placer filling, adopts simultaneously
60co irradiation sterilization, irradiation dose 4kGy;
Preferably, described maize oligopeptide pollen adopts Aluminum-plastic composite bag filling; Ultraviolet or ozonization 50-70min is adopted before described Aluminum-plastic composite bag is filling.
Beneficial effect:
Maize oligopeptide pollen of the present invention take bee pollen of maize as primary raw material, bee pollen of maize extract is obtained through low temperature broken wall, extractive technique, then the composite corn oligopeptide powder of science, modified dietary fiber, fruit vegetable powder, pectin decomposer, chrysanthemum powder, sophora flower powder, isomalt, prepares that a kind of corn fragrance is dense, mouthfeel is suitable for, significantly can strengthen the maize oligopeptide pollen of body immunity, liver protecting.Concrete know-why is:
1. the bee pollen of maize extract that prepared by the present invention is for raw material with bee pollen of maize, waxy corn palpus, defatted corn powder mixture, obtained through ultrasonic cleaning, high-pressure pulse electric and ultrasonic process, biological enzymolysis and fermentation by saccharomyces cerevisiae successively, achieve: 1) ultrasonic cleaning effectively can remove the residues of pesticides of bee pollen of maize, waxy corn palpus, improves the foodsafety of product, 2) high-pressure pulse electric process, has effectively killed the worm's ovum in bee pollen of maize and waxy corn palpus and miscellaneous bacteria, for the enzymolysis of postorder and sweat normally carry out provide good gnotobasis, 3) omnidistance broken wall, extraction (high-pressure pulse electric, ultrasonic, enzymolysis, culture propagation), improve material effective component recovery rate and nutrient composition content, 4) macro-nutrients (protein, starch, fat etc.) both can be converted into Small molecular and easily absorb nutriment by biological enzymolysis, can realize again the broken wall to bee pollen of maize further, 5) the zymotic fluid nutriment composition kind after fermentation by saccharomyces cerevisiae is more, content is higher, be more easily absorbed and utilized by the body, the alcohol of the propagation but also generation that not only achieve probio (saccharomyces cerevisiae) can increase the stripping of alcohol soluble active principals, improves the content of active ingredient, 6) omnidistance employing low temperature extractive technique, effectively prevent the loss of reproducibility active ingredient and volatile fragrant components (in waxy corn palpus), through water extraction and alcohol extracting, the bee pollen of maize of extract not only containing high level and the active ingredient of waxy corn palpus, and containing the probio saccharomyces cerevisiae useful to human body, effectively can promote that human body is to the absorption of nutriment, improve human body integral immunity, also has strong natural corn fragrance simultaneously, significantly improve the corn fragrance of material effective component content and finished product, enhance appetite, improve the uptake ratio of nutritional labeling, digestibility and absorptivity.
2. dietary fiber obtains through physics, chemistry or biological method process by the modified dietary fiber that prepared by the present invention, Soluble Fiber cellulose content is high, biologically active strong, have cellulose that is important, positive role to beneficial bacteria of intestinal tract group, compared with full diet fiber, its biological action is more powerful: 1) Soluble Fiber cellulose content is high, more easily utilized by probio, probio can be improved in the growth of enteron aisle and fertility, increase kind and the quantity of probio flora, reduce large intestine pH value, improve intestine microenvironment; 2) powerful adsorption capacity, through modified, cellulosic specific area increases, and network is enriched, and absorption affinity strengthens, and the organic molecule ability of chelating, absorption cholesterol and bile acids is stronger, suppress human body to their absorption; 3) ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is stronger; 4) the stronger and impact of acid and alkali, alkali, salt of retentiveness, dilatancy, thickening property, 5) resident time of adjustment and maintenance gut flora, strengthen the absorption and digestion ability of enteron aisle, improve body immunity, finally significantly improve the absorption and digestion rate of maize oligopeptide pollen active ingredient; 6) promote gastrointestinal peristalsis, slow down and eliminate the bad reaction such as gasteremphraxis, abdominal distension; 7) bury function is strong, prevents external environment (oxygen, temperature, illumination, humidity etc.) factor from, on the impact of product quality, stabilizing the biologically active of product, extending the shelf-life of product.
3. the pectin decomposer that prepared by the present invention has natural, soft agreeable to the taste tart flavour and significant antagonistic property, especially the strongest to Escherichia coli inhibitory action, also there is stronger non-oxidizability simultaneously, concentration can act on when reaching 0.01%, very strong eliminating effect is all shown to superoxide radical, hydroxy radical and DPPH free radical, when concentration reaches 1.0%, eradicating efficacy reaches 100%; Effectively can improve the palatability of maize oligopeptide pollen, most importantly its significant antagonistic property and non-oxidizability avoid oxidation and the microorganism encroach of maize oligopeptide pollen active ingredient, stabilize and increase the biologically active of maize oligopeptide pollen, extend the shelf-life of product.
4. microwave drying and low-temperature grinding combine by the fruit vegetable powder that prepared by the present invention, remain the nutriment such as color and luster own and cellulose, vitamin, fructose of high microsteping fruits and vegetables to greatest extent, good, with short production cycle compared with the quality of production of other fruit vegetable powder, efficiency is high, be conducive to probio utilize and strengthen intestines peristalsis, be conducive to harmful bacteria and noxious material to excrete, prevent the generation of constipation and symptom of diarrhea.
5. the chrysanthemum powder prepared of the present invention or sophora flower powder adopt low temperature extraction processes such as ultrasonic cleaning, microwave drying and biological enzymolysis and obtained, the active component of chrysanthemum or sophora flower can be retained to greatest extent, keep chrysanthemum or the natural color and luster of sophora flower and fragrance, its nutritional labeling kind is complete, content is high, molecular weight is little, is easy to human consumption and absorption.
6. the isomalt that science of the present invention is composite has low in calories, sugariness is natural pure, height endurability, non-cariogenic tooth, more rational negative heat of solution, high stability and non-hygroscopic, in the quality of improving product, extend the shelf life, promote that gastro-intestinal digestion and absorption aspect have excellent characteristic, simultaneously or a kind of excellent Bifidobacterium MF, although isomalt can not utilize by the enzyme system of human body and most microorganism, but can decompose by the Bifidobacterium in human body intestinal canal utilization, promote the growth and breeding of Bifidobacterium, maintain the microecological balance of enteron aisle, improve human body integral immunity, be conducive to the health of human body.
7. the effect of maize oligopeptide pollen of the present invention is the mutual synergistic result of each component, the not superposition of simple raw material function, the science of each raw material components is composite, and the effect of generation, considerably beyond the superposition of each single component function and effect, has advanced and practicality preferably.
8. preparation method's technique of maize oligopeptide pollen of the present invention is simple, easy to operate, low for equipment requirements; can industrialization and large-scale production; modified dietary fiber is finally mixed, has played the bury function that it is powerful, such that the proterties of product is more stable, the shelf-life is longer.
Detailed description of the invention
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.
Prepared by embodiment 1 raw material
1. the preparation of bee pollen of maize extract
The preparation method of described bee pollen of maize extract, comprise the steps: that 70:5:4 takes bee pollen of maize respectively in mass ratio, waxy corn palpus and defatted corn powder, first by bee pollen of maize, waxy corn must be put in the supersonic wave cleaning machine that 0.4% sodium bicarbonate solution is housed respectively, in 200W under room temperature, 40KHz cleans 4min, drain, then mix with defatted corn powder, add the water of former bee pollen of maize quality 4 times, be 5 by lactic acid adjust ph, in electric-field intensity 30kV/cm, burst length 450 μ s, high-pressure pulse electric process is carried out under pulse frequency 250Hz condition, then room temperature 400W, 40KHz condition ultrasonic process 12min, be warming up to 45 DEG C, add the compound enzyme of former bee pollen of maize quality 5%, stir, enzymolysis 18min, Temperature fall to 30 DEG C, with 4 × 10
5cFU/L enzymolysis liquid inoculum concentration inoculation Active Dry Yeast, fermentation 36h, fermentation liquor 200 eye mesh screen filters, and namely filtrate obtain bee pollen of maize extract through ultrafiltration, reduced pressure concentration, freeze drying,
In described bee pollen of maize extract, active saccharomyces cerevisiae content is 9 × 10
10cFU/g;
The quality group of described compound enzyme becomes: protease: carbohydrase: pectase: cellulase: lipase=12:8:5:4:2
2. the preparation of modified dietary fiber
The preparation method of described modified dietary fiber, comprise the following steps: by inulin, apple fiber, common oats fibre, Semen Tritici aestivi fiber 8:5:4:3 Homogeneous phase mixing in mass ratio, add the water of its quality 5 times, room temperature 300W, 40KHz condition ultrasonic extraction 12min, then at electric-field intensity 30kV/cm, burst length 500 μ s, carries out high-pressure pulse electric extraction under pulse frequency 300Hz condition; Be 5.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.2%, in 50 DEG C of enzymolysis 35min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to particle diameter are that namely 0.2mm obtains modified dietary fiber;
Described biology enzyme is zytase, cellulase, laccase, pectase, tannase, amylase and protease 7:4:3:3:3:2:0.3 Homogeneous phase mixing in mass ratio.
3. the preparation of pectin decomposer
The preparation method of described pectin decomposer, fresh hawthorn pomace, Grape Skin, pomace, megasse is comprised the steps: to put into the supersonic wave cleaning machine that 1.2% sodium bicarbonate solution is housed respectively and clean 8min in 200W, 40KHz, drain, be cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, in mass ratio 8:6:3:3 Homogeneous phase mixing, then in-23 DEG C of freezing 20min, pulverize immediately, particle diameter is 0.5mm, add the deionized water of crushed material quality 4 times, mix, be 2.5 by citric acid adjust ph, at electric-field intensity 40kV/cm under room temperature, burst length 500 μ s, high-pressure pulse electric process is carried out under pulse frequency 300Hz condition, then normal pressure boils 40min, Bag filter, the filter residue filtrate normal pressure of its quality 2 times boils 50min, Bag filter, merging filtrate, diatomite filtration, filtrate is clarified, at 75 DEG C, be evaporated to solid content is 8%, be cooled to room temperature rapidly, the pH value adding concentrate 1.5 times of volumes is 95% ethanol of 2.5, leave standstill 25min, pectin is precipitated out, the centrifugal 8min of 4000r/min, reclaim precipitation, with the absolute ethyl alcohol wash-out of 2 times of volumes, centrifugal, reclaim precipitation, operation like this 2 times, what obtain is deposited in 50 DEG C of vacuum drying 2.5h, obtain pectin, add 45 DEG C of water of pectin quality 5 times, stir, Homogeneous phase mixing, insulation, be 5 by lactic acid adjust ph, add the pectinase enzymatic hydrolysis 3h of 0.5U/mL, enzymolysis liquid filters, filtrate is gone out enzyme 12min in boiling water, be cooled to room temperature, ultrafiltration concentration, namely freeze drying obtains pectin decomposer.
4. the preparation of fruit vegetable powder
The mass percent of described fruit vegetable powder consists of: acerola concentrate powder 15%, lemon powder 15%, blueberry powder 12%, blackberry powder 10%, Cranberry powder 8%, raspberry powder 8%, papaya powder 5%, mango powder 5%, leek powder 5%, white radish powder 3%, white gourd powder 3%, mung bean sprouts powder 3%, carrot meal 2%, celery powder 2%, pineapple powder 2%, orange powder 2%;
Namely above-mentioned fruit vegetable powder Homogeneous phase mixing obtains fruit vegetable powder;
The preparation method of each fruit vegetable powder described is: put by fruits and vegetables in the supersonic wave cleaning machine that 1.2% sodium bicarbonate solution is housed and clean 12min in 200W, 40KHz, drain, be cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, put in microwave dryer and carry out microwave drying 15min, until moisture is down to 7% under power 3000W, frequency 2450MHz, temperature 55 DEG C of condition, then be crushed to particle diameter 0.2mm in 25 DEG C, pack and obtain fruit vegetable powder.
5. the preparation of chrysanthemum powder and sophora flower powder
The preparation method of described chrysanthemum powder or sophora flower powder is: by the fresh chrysanthemum of harvesting or sophora flower impurity elimination, put into 0.7% sodium bicarbonate solution is housed supersonic wave cleaning machine in 200W, 40KHz cleans 12min, drain, put in microwave dryer in power 3000W, frequency 2450MHz, microwave drying 10min is carried out under temperature 55 DEG C of conditions, until moisture is down to 9%, then be crushed to particle diameter 0.2mm in 25 DEG C and must pulverize pollen, add the water pulverizing pollen quality 2 times, be 5 by lactic acid adjust ph, in electric-field intensity 30kV/cm, burst length 500 μ s, high-pressure pulse electric process is carried out under pulse frequency 300Hz condition, then room temperature 400W, 40KHz condition ultrasonic process 12min, be warming up to 45 DEG C, add the mixed enzyme pulverizing pollen quality 4%, stir, enzymolysis 18min, enzymolysis liquid filters through 200 eye mesh screens, and namely filtrate obtain chrysanthemum powder or sophora flower powder through ultrafiltration, reduced pressure concentration, freeze drying,
The quality group of described mixed enzyme becomes: protease: pectase: cellulase: lipase=12:8:5:4.
The bee pollen of maize extract that following embodiment 2-6 uses, modified dietary fiber, fruit vegetable powder, pectin decomposer, chrysanthemum powder, sophora flower powder are embodiment 1 to be prepared, and other is commercial products.
Embodiment 2
A kind of maize oligopeptide pollen, is prepared by the raw material of following parts by weight: bee pollen of maize extract 85 parts, corn oligopeptide powder 20 parts, modified dietary fiber 15 parts, fruit vegetable powder 16 parts, pectin decomposer 12 parts, chrysanthemum powder 9 parts, 6 parts, sophora flower powder, isomalt 6 parts;
The preparation method of described maize oligopeptide pollen, comprise the steps: according to formula rate, accurately take each raw material, successively isomalt, sophora flower powder, chrysanthemum powder, pectin decomposer, fruit vegetable powder, corn oligopeptide powder, bee pollen of maize extract are added V-type blending tank Homogeneous phase mixing 30min, speed of agitator 30r/min, then modified dietary fiber evenly mixed 15min is added, speed of agitator 50r/min, cross 60 mesh sieves, sterile filling, sealing, packaging get product maize oligopeptide pollen;
Described sterile filling adopts automatic powdery bottle placer filling, adopts simultaneously
60co irradiation sterilization, irradiation dose 4kGy;
Described maize oligopeptide pollen adopts Aluminum-plastic composite bag filling; Disinfection by ultraviolet light 60min is adopted before described Aluminum-plastic composite bag is filling.
Embodiment 3
A kind of maize oligopeptide pollen, is prepared by the raw material of following parts by weight: bee pollen of maize extract 75 parts, corn oligopeptide powder 10 parts, modified dietary fiber 10 parts, fruit vegetable powder 10 parts, pectin decomposer 5 parts, chrysanthemum powder 3 parts, 2 parts, sophora flower powder, isomalt 2 parts;
The preparation method of described maize oligopeptide pollen, comprise the steps: according to formula rate, accurately take each raw material, successively isomalt, sophora flower powder, chrysanthemum powder, pectin decomposer, fruit vegetable powder, corn oligopeptide powder, bee pollen of maize extract are added V-type blending tank Homogeneous phase mixing 20min, speed of agitator 20r/min, then modified dietary fiber Homogeneous phase mixing 10min is added, speed of agitator 40r/min, cross 60 mesh sieves, sterile filling, sealing, packaging get product maize oligopeptide pollen;
Described sterile filling adopts automatic powdery bottle placer filling, adopts simultaneously
60co irradiation sterilization, irradiation dose 4kGy;
Described maize oligopeptide pollen adopts Aluminum-plastic composite bag filling; Ozonization 50min is adopted before described Aluminum-plastic composite bag is filling.
Embodiment 4
A kind of maize oligopeptide pollen, is prepared by the raw material of following parts by weight: bee pollen of maize extract 95 parts, corn oligopeptide powder 30 parts, modified dietary fiber 20 parts, fruit vegetable powder 20 parts, pectin decomposer 20 parts, chrysanthemum powder 12 parts, 10 parts, sophora flower powder, isomalt 10 parts;
The preparation method of described maize oligopeptide pollen, comprise the steps: according to formula rate, accurately take each raw material, successively isomalt, sophora flower powder, chrysanthemum powder, pectin decomposer, fruit vegetable powder, corn oligopeptide powder, bee pollen of maize extract are added V-type blending tank Homogeneous phase mixing 40min, speed of agitator 40r/min, then modified dietary fiber Homogeneous phase mixing 20min is added, speed of agitator 60r/min, cross 60 mesh sieves, sterile filling, sealing, packaging get product maize oligopeptide pollen;
Described sterile filling adopts automatic powdery bottle placer filling, adopts simultaneously
60co irradiation sterilization, irradiation dose 4kGy;
Described maize oligopeptide pollen adopts Aluminum-plastic composite bag filling; Disinfection by ultraviolet light 70min is adopted before described Aluminum-plastic composite bag is filling.
Embodiment 5
A kind of maize oligopeptide pollen, is prepared by the raw material of following parts by weight: bee pollen of maize extract 80 parts, corn oligopeptide powder 15 parts, modified dietary fiber 13 parts, fruit vegetable powder 14 parts, pectin decomposer 10 parts, chrysanthemum powder 8 parts, 4 parts, sophora flower powder, isomalt 4 parts;
The preparation method of described maize oligopeptide pollen, comprise the steps: according to formula rate, accurately take each raw material, successively isomalt, sophora flower powder, chrysanthemum powder, pectin decomposer, fruit vegetable powder, corn oligopeptide powder, bee pollen of maize extract are added V-type blending tank Homogeneous phase mixing 20min, speed of agitator 40r/min, then modified dietary fiber Homogeneous phase mixing 10min is added, speed of agitator 60r/min, cross 60 mesh sieves, sterile filling, sealing, packaging get product maize oligopeptide pollen;
Described sterile filling adopts automatic powdery bottle placer filling, adopts simultaneously
60co irradiation sterilization, irradiation dose 4kGy;
Described maize oligopeptide pollen adopts Aluminum-plastic composite bag filling; Ozonization 60min is adopted before described Aluminum-plastic composite bag is filling.
Embodiment 6
A kind of maize oligopeptide pollen, is prepared by the raw material of following parts by weight: bee pollen of maize extract 90 parts, corn oligopeptide powder 25 parts, modified dietary fiber 17 parts, fruit vegetable powder 18 parts, pectin decomposer 15 parts, chrysanthemum powder 10 parts, 8 parts, sophora flower powder, isomalt 8 parts;
The preparation method of described maize oligopeptide pollen, comprise the steps: according to formula rate, accurately take each raw material, successively isomalt, sophora flower powder, chrysanthemum powder, pectin decomposer, fruit vegetable powder, corn oligopeptide powder, bee pollen of maize extract are added V-type blending tank Homogeneous phase mixing 30min, speed of agitator 30r/min, then modified dietary fiber Homogeneous phase mixing 15min is added, speed of agitator 50r/min, cross 60 mesh sieves, sterile filling, sealing, packaging get product maize oligopeptide pollen;
The sensory evaluation test of embodiment 7 maize oligopeptide pollen of the present invention
The maize oligopeptide pollen of inviting 24 personnel to prepare embodiment of the present invention 2-6 and commercially available two kinds of bee pollen of maize are judged, sense organ is given a mark, wherein specialty and each 12 of layman, professional is young, middle aged, each 4 of old age, men and women half and half, layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half; Marking comprises outward appearance (20 points), quality (25 points), local flavor (30 points), mouthfeel (25 points) four aspects, and marking personnel independently carry out, and are independent of each other, accurate to ensure judging result.Add up judging result, equal score value gets approximation, retains integer, specifically in table 1:
Table 1 sensory evaluation statistics
Note: show significant difference (P < 0.05) with marking different lowercase alphabet in a line, mark different capitalization and represent that difference extremely significantly (P < 0.01), indicate same letter and represent difference not significantly (P > 0.05).
Above result shows, any one all will obviously be better than commercially available bee pollen of maize to maize oligopeptide pollen prepared by the present invention from outward appearance, quality, local flavor and mouthfeel, particularly local flavor is fabulous, have natural corn fragrance, also the consumer of applicable Different age group, different hierarchy of consumption eats simultaneously.
The storage stability of embodiment 8 maize oligopeptide pollen
The same specification bee pollen of maize of maize oligopeptide pollen and the commercially available identical date of manufacture of preparing with the embodiment of the present invention 2 is for subjects, under identical storage condition (room temperature, ventilation, lucifuge), detect the sanitary indexs such as total plate count, Escherichia coli, pathogenic bacteria, remains of pesticide, heavy metal by relevant Bee Pollen standard (pollution-free food that the Ministry of Agriculture issues---Bee Pollen quality standard), result is as table 2:
Table 2 stores 12 months sanitary index testing results
| Test item | Standard | Commercially available | The present invention |
| Total plate count (cfu/g)≤ | 1000 | 1230 | 16 |
| Coliform (MPN/100g)≤ | 30 | 56 | Do not detect |
| Pathogenic bacteria (pathogenic entero becteria, pathogenic coccus) | Must not detect | Do not detect | Do not detect |
| Total number of molds (cfu/g)≤ | 200 | Do not detect | Do not detect |
| Plumbous (in Pb) (mg/kg)≤ | 1 | 0.7 | Do not detect |
| Arsenic (in As) (mg/kg)≤ | 0.3 | 0.21 | Do not detect |
| Mercury (mg/kg)≤ | 0.3 | Do not detect | Do not detect |
| BHC (mg/kg)≤ | 0.05 | 0.018 | Do not detect |
| DDT (mg/kg)≤ | 0.05 | 0.013 | Do not detect |
Above result shows: when commercially available bee pollen of maize is stored to 12 months, and its total plate count and coliform exceed standard, and content of microorganisms increases progressively rapidly, maize oligopeptide pollen contamination miscellaneous bacteria kind of the present invention is few, every Testing index stores 12 and is monthlyly better than national standard, and along with the prolongation in storage time, content of microorganisms propagation slowly, show the microbial stability that maize oligopeptide pollen of the present invention is excellent, maize oligopeptide pollen preparation process of the present invention have employed ultrasonic cleaning to exotic plant raw material simultaneously, effectively reduce agriculture residual, the content of toxin and heavy metal ion, although commercially available bee pollen of maize is qualified, but there is larger food security hidden danger, long-term edible meeting cause the serious harm of accumulation property to human body.
After testing, it is little with embodiment 2 sanitary index testing result difference that maize oligopeptide pollen prepared by embodiment of the present invention 3-6 stores 12 months, has excellent foodsafety and microbial stability equally.
Embodiment 9 maize oligopeptide pollen of the present invention is on the impact of alcoholic liver injury
1. experiment material
1.1 animal used as test Kunming mouses, regular grade, body weight 22.5 ± 2.5g, male and female have concurrently, are provided by Chinese Medical Sciences University's animal experimental center.
Maize oligopeptide pollen prepared by the 1.2 experimental drug embodiment of the present invention 2, is mixed with warm water the solution that mass percent concentration is 8% by it.
1.3 main agents trace MDA (MDA) N measure kit, reduced glutathione (GSH) measures kit.
1.4 laboratory apparatus 722s type spectrophotometers, electronic balance.
2. experimental technique
2.1 grouping and medication experiment mices 50, are divided into 5 groups at random, often organize 10.Be respectively basic, normal, high group of administration (1,2,4g/kg), positive controls and model control group.Equal ig administration.The isopyknic physiological saline of model group ig.
Before 2.2 experimental program administrations, the equal ig38 degree white wine (15mL/kg) of each group, then administration, puts to death each group of mouse after 6h, get a fritter hepatic tissue of right lobe of liver immediately, adds the LH that 4 DEG C of physiological saline make 10%.Centrifugal 10 minutes of 2000r/min, divides and gets supernatant, measures the content of glutathione (GSH) and MDA (MDA).The results are shown in Table 3.
2.3 statistical procedures data represent with ± s, and application SPSS12.0 statistical package processes.Compare employing variance analysis between group, P < 0.05 has statistical significance.
After table 3 administration on the impact of mouse liver injury (
n=10)
| Group | MDA(nmol/mg) | GSH(ug/mg) |
| Model group | 2.11±0.35 | 1.74±0.17 |
| Positive controls | 1.62±0.51 ＊ | 1.94±0.21 ＊ |
| Administration high dose group | 1.50±0.39 ＊ | 1.92±0.35 ＊ |
| Dosage group in administration | 1.53±0.25 ＊ | 1.96±0.21 ＊ |
| Administration low dose group | 1.88±0.28 ＊ | 1.76±0.20 |
Compare with model group,
*p < 0.05.
Above result display: maize oligopeptide pollen of the present invention significantly can not only reduce the MDA content after mice drunk in liver, and greatly can also raise the content of GSH.Illustrate maize oligopeptide pollen of the present invention have sober up, the effect of liver protecting, it is relevant with dosage that experimental result also shows above-mentioned effect, and middle and high Dose Results is unanimously and be all better than low dosage.
It should be noted that: maize oligopeptide pollen prepared by embodiment of the present invention 3-6 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect otherness.
Embodiment 10 maize oligopeptide pollen of the present invention is on the impact of immunity of organisms
1 experiment purpose
By exercise tolerance test (mouse forced swimming), verify raising immunity, the antifatigue effect of maize oligopeptide pollen of the present invention.
2 experiment materials and reagent
2.1 for reagent thing:
Commercially available bee pollen of maize (G1); Commercially available bee pollen of maize (G2); Maize oligopeptide pollen (G3-G7) prepared by embodiment of the present invention 2-6.
2.2 reagent:
Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing; The concentrated sulfuric acid (AR), Nanjing Chemistry Reagent Co., Ltd.; Physiological saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..
3. animal used as test
ICR mouse, ♂, cleaning grade, body weight 18-22g, is provided by Yangzhou University's comparative medicine center, the quality certification number: SCXK (Soviet Union) 2007 – 0001, the free diet of experimental session mouse.
4. key instrument
Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof company; Water-bath: DK-S26 type, the grand experimental facilities Co., Ltd of upper Nereid; Electronic scale: BS224S type, Sartorius company; Stopwatch, thermometer
5. experiment grouping
5.1 dosage groupings and given the test agent give the time and at random mouse are divided into 8 groups, often organize 10,1st group to the 7th group respectively to the medicine of G1 ~ G7,8th group is blank group, give isopyknic distilled water, the often every average daily gavage of group 1 time, gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.
5.2 sample preparations the 1st group to the 7th group: take 2.25g drug sample, be assigned to 150ml with distilled water; Blank group: distilled water 150ml.
6. experimental technique
After 6.1 swimming with a load attached to the body experiment last administration 30min, put mouse in swimming trunk, the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of mouse root of the tail portion load 5% body weight, and the swimming of record mouse starts to the dead time, as mouse swimming time.
After 6.2 mice serum urea measure last administration 30min, be not swimming with a load attached to the body 90min in the water of 30 DEG C in temperature, eyeball blood sampling 0.5mL (not adding anti-coagulants) is plucked after rest 60min, put 4 DEG C of refrigerator 3h, the centrifugal 15min of 2000r/min after blood clotting, gets serum and send clinical laboratory of Lianyungang First People's Hospital to detect.
After the mensuration last administration 30min of 6.3 hepatic glycogen, be not swimming with a load attached to the body 90min in the water of 25 ± 1 DEG C in temperature, cervical dislocation puts to death mouse, clean with physiological saline, and with after filter paper suck dry moisture, accurately taking liver 100mg, hepatic glycogen detection kit detects Mouse Liver glycogen content.
Take a blood sample after the mensuration last administration 30min of 6.4 blood lactase acid, then do not bear a heavy burden stops after temperature is the water went swimming 10min of 30 DEG C.Lactic acid instrument assay method: after rest 20min, each blood sampling 20 μ L add in 40 μ L rupture of membranes liquid before swimming, after swimming, after swimming respectively, the smudge cells lactic acid instrument that fully vibrates immediately measures.(blood lactase acid TG-AUC=5 × (the blood lactase acid value of the rear 20min of blood lactase acid value+2 × swimming of the rear 0min of front blood lactase acid value+3 × swimming that swims)
7. observation index walking weight load, blood lactase acid, urea, glycogen initial value
8. statistical method experimental data is used
represent, employing t inspection is compared between organizing
9. experimental result
The impact of 9.1 maize oligopeptide Effects of Pollen in Mice body weight of the present invention
Each group of mouse is after giving G1 ~ G7 medicine, before, in, shown in post-weight sees the following form respectively, the original body mass of each group mouse and increase weight body weight and the more equal no difference of science of statistics of control group (P > 0.05), show G1 ~ G7 medicine all without obvious toxicity.Experimental result refers to table 4.
The original body mass of table 4 swimming with a load attached to the body experiment mice, mid-term body weight and terminate body weight
The impact of 9.2 maize oligopeptide Effects of Pollen in Mice walking weight load of the present invention
After per os gives mouse G1 ~ G7 medicine, G1 ~ G2 medicine compares with blank group, can obviously extend the mice burden swimming time, there is significant difference (P < 0.05), maize oligopeptide pollen G3 ~ G7 medicine of the present invention compares with blank group, can the significant prolongation mice burden swimming time, there is pole significant difference (P < 0.01), and be obviously better than G1 ~ G2 medicine.The results detailed in Table 5.
The impact of table 5 maize oligopeptide Effects of Pollen in Mice of the present invention walking weight load
" * " p<0.05vs blank;
" * * " p<0.01vs blank;
The impact of blood lactase acid before and after 9.3 maize oligopeptide Effects of Pollen in Mice motions of the present invention
After per os gives mouse maize oligopeptide pollen of the present invention, maize oligopeptide pollen G3 ~ G7 medicine of the present invention to compare with control group blood lactase acid TG-AUC after mouse movement significant difference (P < 0.05), decrease though G1 ~ G2 medicine group Mouse Blood lactic acid TG-AUC compares with control group, and no difference of science of statistics (P > 0.05).The results are shown in Table 6.
The impact of blood lactase acid level before and after table 6 maize oligopeptide Effects of Pollen in Mice motion of the present invention
" * " p<0.05vs blank;
The impact of 9.4 maize oligopeptide Effects of Pollen in Mice hepatic glycogen of the present invention
After per os gives mouse G1 ~ G7 medicine, G1 ~ G2 medicine compares with blank group, Mouse Liver glycogen content all has obvious rising, there is significant difference (P < 0.05), maize oligopeptide pollen G3 ~ G7 medicine of the present invention compares with blank group, Mouse Liver glycogen content all has obvious rising, has pole significant difference (P < 0.01), and is obviously better than G1 ~ G2 medicine.The results detailed in Table 7.
The impact of table 7 maize oligopeptide Effects of Pollen in Mice of the present invention hepatic glycogen content
" * " p<0.05vs blank;
" * * " p<0.01vs blank;
The impact of 9.5 maize oligopeptide Effects of Pollen in Mice serum ureas of the present invention
After per os gives mouse G1 ~ G7 medicine, G1 ~ G2 medicine group compares with blank group, after mouse movement, serum urea content all has obvious reduction, there is significant difference (P < 0.05), maize oligopeptide pollen G3 ~ G7 medicine of the present invention compares with blank group, after mouse movement, serum urea content all has obvious reduction all to have obvious reduction, there is pole significant difference (P < 0.01), and be obviously better than G1 ~ G2 medicine.The results detailed in Table 8.
The impact of table 8 maize oligopeptide Effects of Pollen in Mice of the present invention serum urea content
" * " p<0.05vs blank;
" * * " p<0.01vs blank;
10. experiment conclusion
This experiment is mainly through mice burden swimming experiment, and the deposit simultaneously detecting Mouse Liver glycogen observes the antifatigue effect of maize oligopeptide pollen of the present invention.Preliminary Results shows below:
1, G3 ~ G7 maize oligopeptide pollen of the present invention all can extend the mice burden swimming time (P < 0.01), and successful is better than the bee pollen of maize of other G1 ~ G2.
2, biochemistry detection aspect display, the lactic acid content that after each dosage group of G3 ~ G7 maize oligopeptide pollen of the present invention all can reduce motion, in mice serum, glucose anerobic glycolysis produces, compare with control group and have significant difference (P < 0.05), and although the bee pollen of maize of other G1 ~ G2 also can reduce the lactic acid content that in the rear mice serum of motion, glucose anerobic glycolysis produces, but compare with control group, no difference of science of statistics (P > 0.05);
3, each dosage group of G3 ~ G7 maize oligopeptide pollen of the present invention all can significantly improve the deposit (P < 0.01) of glycogen in mouse liver, and successful is better than the bee pollen of maize of other G1 ~ G2;
4, high lithemia model finds, G3 ~ G7 maize oligopeptide pollen of the present invention significantly can reduce the content (P < 0.01) of the rear urea in serum of mouse swimming, and successful is better than other G1 ~ G bee pollen of maize;
11. conclusions
Above-mentioned experiment proves that maize oligopeptide pollen of the present invention can significantly improve immunity of organisms, improve muscle power and the endurance of decimal, the content of urea in serum and lactic acid after reduction mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, contribute to the fatigue that alleviation exercise load causes; The time that mice burden swimming to power exhausts can be extended.
Claims (10)
1. a maize oligopeptide pollen, prepared by the raw material of following parts by weight: bee pollen of maize extract 75-95 part, corn oligopeptide powder 10-30 part, modified dietary fiber 10-20 part, fruit vegetable powder 10-20 part, pectin decomposer 5-20 part, chrysanthemum powder 3-12 part, sophora flower powder 2-10 part, isomalt 2-10 part.
2. maize oligopeptide pollen as claimed in claim 1, it is characterized in that, prepared by the raw material of following parts by weight: bee pollen of maize extract 80-90 part, corn oligopeptide powder 15-25 part, modified dietary fiber 13-17 part, fruit vegetable powder 14-18 part, pectin decomposer 10-15 part, chrysanthemum powder 8-10 part, sophora flower powder 4-8 part, isomalt 4-8 part.
3. maize oligopeptide pollen as claimed in claim 1, it is characterized in that, prepared by the raw material of following parts by weight: bee pollen of maize extract 85 parts, corn oligopeptide powder 20 parts, modified dietary fiber 15 parts, fruit vegetable powder 16 parts, pectin decomposer 12 parts, chrysanthemum powder 9 parts, 6 parts, sophora flower powder, isomalt 6 parts.
4. maize oligopeptide pollen as claimed in claim 1, it is characterized in that, the preparation method of described bee pollen of maize extract comprises the steps: that 60-80:3-8:2-6 takes bee pollen of maize respectively in mass ratio, waxy corn palpus and defatted corn powder, first by bee pollen of maize, waxy corn must be put in the supersonic wave cleaning machine that 0.3-0.5% sodium bicarbonate solution is housed respectively, in 200W under room temperature, 40KHz cleans 3-5min, drain, then mix with defatted corn powder, add former bee pollen of maize quality 3-5 water doubly, be 4-6 by lactic acid adjust ph, in electric-field intensity 20-40kV/cm, burst length 400-500 μ s, high-pressure pulse electric process is carried out under pulse frequency 200-300Hz condition, then room temperature 400W, 40KHz condition ultrasonic process 10-15min, be warming up to 40-50 DEG C, add the compound enzyme of former bee pollen of maize quality 2-8%, stir, enzymolysis 15-20min, Temperature fall to 28-35 DEG C, with 3 × 10
5-5 × 10
5cFU/L enzymolysis liquid inoculum concentration inoculation Active Dry Yeast, fermentation 20-48h, fermentation liquor 100-300 eye mesh screen filters, and namely filtrate obtain bee pollen of maize extract through ultrafiltration, reduced pressure concentration, freeze drying,
The quality group of described compound enzyme becomes: protease: carbohydrase: pectase: cellulase: lipase=10-15:5-10:4-6:3-5:1-3;
In described bee pollen of maize extract, active saccharomyces cerevisiae content is 6 × 10
10-9 × 10
10cFU/g.
5. maize oligopeptide pollen as claimed in claim 1, it is characterized in that, the preparation method of described modified dietary fiber comprises the following steps: by inulin, apple fiber, common oats fibre, Semen Tritici aestivi fiber 6-11:4-6:3-5:2-4 Homogeneous phase mixing in mass ratio, add its quality 3-7 water doubly, room temperature 200-300W, 35-40KHz condition ultrasonic extraction 10-15min, then at electric-field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric extraction under pulse frequency 200-300Hz condition; Be 4.5-6.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains modified dietary fiber;
Described biology enzyme is zytase, cellulase, laccase, pectase, tannase, amylase and protease 5-9:2-5:2-4:2-4:2-4:1-3:0.2-0.4 Homogeneous phase mixing in mass ratio.
6. maize oligopeptide pollen as claimed in claim 1, it is characterized in that, the preparation method of described pectin decomposer comprises the steps: fresh hawthorn pomace, Grape Skin, pomace, megasse to put into the supersonic wave cleaning machine that 1.2% sodium bicarbonate solution is housed respectively and clean 5-10min in 200W, 40KHz, drain, be cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, 6-9:5-8:2-4:2-4 Homogeneous phase mixing in mass ratio, then in-21--25 DEG C of freezing 10-30min, pulverize immediately, particle diameter is 0.1-1mm, add crushed material quality 3-5 deionized water doubly, mix, be 2-3 by citric acid adjust ph, at electric-field intensity 35-45kV/cm under room temperature, burst length 400-600 μ s, high-pressure pulse electric process is carried out under pulse frequency 200-300Hz condition, then normal pressure boils 30-50min, Bag filter, filter residue its quality 1-3 filtrate normal pressure doubly boils 40-60min, Bag filter, merging filtrate, diatomite filtration, filtrate is clarified, at 75 DEG C, be evaporated to solid content is 6-10%, be cooled to room temperature rapidly, the pH value adding concentrate 1.5 times of volumes is 95% ethanol of 2-3, leave standstill 20-30min, pectin is precipitated out, the centrifugal 5-10min of 4000r/min, reclaim precipitation, with the absolute ethyl alcohol wash-out of 2 times of volumes, centrifugal, reclaim precipitation, operation like this 2 times, what obtain is deposited in 40-60 DEG C of vacuum drying 2-3h, obtain pectin, add pectin quality 4-5 40-50 DEG C of water doubly, stir, Homogeneous phase mixing, insulation, be 4.5-5.5 by lactic acid adjust ph, add the pectinase enzymatic hydrolysis 2-4h of 0.3-0.8U/mL, enzymolysis liquid filters, filtrate is gone out enzyme 10-15min in boiling water, be cooled to room temperature, ultrafiltration concentration, namely freeze drying obtains pectin decomposer.
7. maize oligopeptide pollen as claimed in claim 1, it is characterized in that, the mass percent of described fruit vegetable powder consists of: acerola concentrate powder 15%, lemon powder 15%, blueberry powder 12%, blackberry powder 10%, Cranberry powder 8%, raspberry powder 8%, papaya powder 5%, mango powder 5%, leek powder 5%, white radish powder 3%, white gourd powder 3%, mung bean sprouts powder 3%, carrot meal 2%, celery powder 2%, pineapple powder 2%, orange powder 2%;
The preparation method of each fruit vegetable powder above-mentioned is: put by fruits and vegetables in the supersonic wave cleaning machine that 1.2% sodium bicarbonate solution is housed and clean 10-15min in 200W, 40KHz, drain, be cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, put in microwave dryer and carry out microwave drying 5-25min under power 3000W, frequency 2450MHz, temperature 50-60 DEG C condition, until moisture is down to 5-8%, then be crushed to particle diameter 0.1-0.3mm in 20-30 DEG C, pack and obtain fruit vegetable powder.
8. the preparation method of maize oligopeptide pollen as described in as arbitrary in claim 1-7, it is characterized in that, comprise the steps: according to formula rate, accurately take each raw material, successively by isomalt, sophora flower powder, chrysanthemum powder, pectin decomposer, fruit vegetable powder, corn oligopeptide powder, bee pollen of maize extract adds V-type blending tank Homogeneous phase mixing 20-40min, speed of agitator 20-40r/min, then modified dietary fiber Homogeneous phase mixing 10-20min is added, speed of agitator 40-60r/min, cross 60 mesh sieves, sterile filling, sealing, pack the maize oligopeptide pollen that gets product.
9. the preparation method of maize oligopeptide pollen as claimed in claim 8, is characterized in that, described maize oligopeptide pollen adopts Aluminum-plastic composite bag filling; Ultraviolet or ozonization 50-70min is adopted before described Aluminum-plastic composite bag is filling; Described sterile filling adopts automatic powdery bottle placer filling, adopts simultaneously
60co irradiation sterilization, irradiation dose 4kGy.
10. the preparation method of maize oligopeptide pollen as claimed in claim 8, it is characterized in that, the preparation method of described chrysanthemum powder or sophora flower powder is: by the fresh chrysanthemum of harvesting or sophora flower impurity elimination, put into 0.6-0.8% sodium bicarbonate solution is housed supersonic wave cleaning machine in 200W, 40KHz cleans 10-15min, drain, put in microwave dryer in power 3000W, frequency 2450MHz, microwave drying 5-15min is carried out under temperature 50-60 DEG C of condition, until moisture is down to 8-10%, then be crushed to particle diameter 0.1-0.3mm in 20-30 DEG C and must pulverize pollen, add and pulverize pollen quality 1-3 water doubly, be 4-6 by lactic acid adjust ph, in electric-field intensity 20-40kV/cm, burst length 400-500 μ s, high-pressure pulse electric process is carried out under pulse frequency 200-300Hz condition, then room temperature 400W, 40KHz condition ultrasonic process 10-15min, be warming up to 40-50 DEG C, add the mixed enzyme pulverizing pollen quality 3-5%, stir, enzymolysis 15-20min, enzymolysis liquid filters through 100-300 eye mesh screen, and namely filtrate obtain chrysanthemum powder or sophora flower powder through ultrafiltration, reduced pressure concentration, freeze drying,
The quality group of described mixed enzyme becomes: protease: pectase: cellulase: lipase=10-15:5-10:4-6:3-5.
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