CN105076649A

CN105076649A - Blueberry pressed candies capable of delaying ageing and preparation method of blueberry pressed candies

Info

Publication number: CN105076649A
Application number: CN201510582611.6A
Authority: CN
Inventors: 李绩
Original assignee: BEIJING KEHUITONG WISDOM TECHNOLOGY Co Ltd
Current assignee: BEIJING KEHUITONG WISDOM TECHNOLOGY Co Ltd
Priority date: 2015-09-14
Filing date: 2015-09-14
Publication date: 2015-11-25

Abstract

The invention discloses blueberry pressed candies capable of delaying ageing and a preparation method of the blueberry pressed candies and belongs to the technical field of deep processing of blueberries. The blueberries are used as main raw materials and are processed into blueberry paste by adopting a non-heat processing method; then the blueberry paste is uniformly mixed with milk, sucrose, Chinese herbal medicine extract, honey and a stabilizer, and then a fermentation agent is added to prepare blueberry yoghourt by adopting a stepwise temperature-changing manner, so that probiotics are proliferated to the greatest extent, and the cold and heat stress resisting capability is relatively good; then protecting agent freeze-dried powder with relatively good anti-freezing effect is added to obtain functional blueberry yoghourt freeze-dried powder with high viable bacterium content of the probiotics and strong cold and heat resisting capabilities; and finally, the functional blueberry yoghourt freeze-dried powder is scientifically compounded with alfalfa extract, germinated brown rice, plant extract, dextrin, probiotic factors, magnesium stearate and the like, and the mixture is pressed by a multi-punch rotary type pressing machine to prepare the blueberry pressed candies which have the high viable bacterium content of the probiotics, good stability and long guarantee period, are capable of improving the human immunity, have the effects of maintaining beauty and keeping young and resisting bacteria, and can delay the ageing.

Description

Blueberry compressed tablet candy of a kind of delaying sanility and preparation method thereof

Technical field

The present invention relates to pressed candy, particularly a kind of blueberry compressed tablet candy and preparation method thereof of delaying sanility.

Background technology

Blueberry, also known as cowberry, is commonly called as city fruit, and be perennial fallen leaves or long green fruit tree, cold resistance is extremely strong, can resist the severe cold of subzero 50 DEG C, originates in North America, Scotland and Russia, is a kind of emerging worldwide small berries fruit tree with high economic worth.Blueberry is the abundantest kind of Vaccinium plant Middle nutrition composition, and its fruit is berry, and in blue, subcircular, pulp is fine and smooth, sweet acid appropriateness.Fruit, except containing except sugar, acid and vitamin C, is also rich in vitamin E, vitamin A, Cobastab ₁, SOD, Arbutin, anthocyanin, protein, the trace element such as rare special composition and abundant iron, zinc, manganese in other fruits such as fat.According to scientific research, Blueberry have prevent cranial nerve old and feeble, strengthen the unique effects such as cardiac function, improving eyesight be anticancer.

Blueberry contains a large amount of material to human health, comprises antimicrobial component, polyphenoils, tannic acid, folic acid and dietary fibre etc.At present, studying more is flavone compound in blueberry.Research finds, flavone compound has multiple biologically active and pharmic function, and as removed interior free yl, anti-inflammatory, reduction cardiovascular disease incidence rate, and inhibition cancer cell grows and promotes the effects such as its apoptosis.Anthocyanin in Cranberry and blueberry, flavonols, PCA, cinnamic acid and talan, triterpene compound (ursolic acid ursolicacid) etc. can suppress vascular sclerosis, blood loss apoplexy, old nerve degeneration etc.Therefore, blueberry is described as " king of berry ", one of five large healthy fruit that Ye Shi the World Food Programme is recommended.

Along with improving constantly of our people's living standard, more and more higher to food requirement, the secondary industry of blueberry will have vast potential for future development, and it will become another new industry of 21 century.In recent years, along with people to the pay attention to day by day of health and people going deep into blueberry mixed health function understanding, the exploitation of blueberry food cause people and greatly pay close attention to.Domestic is at present that the food variety of development of raw materials is various with Blueberry, mainly contains blueberry dairy products, fruit drink, fruit beer, fruit wine, jelly, jam, soft sweets, fruitcake and some leisure food etc.

Research abroad for active component in blueberry mainly concentrates on anthocyanin, have studied many physiological functions and the oxidation resistance thereof of Anthocyanin-rich Extract; Also research is had for the functional of active component in blueberry.And the domestic research for blueberry also mainly concentrates on introduction cultivation technique, the research for the active component in blueberry and the mechanism of action is deep not enough, and rarely has report to the activity research of the aldehydes matter in blueberry.Studies have reported that the aldehydes matter in blueberry has bacteriostasis, adopt alcohol extracting method to extract aldehydes matter from blueberry, then after preliminary purification, carry out the research of the bacteriostasis of aldehydes matter, for the production of aldehydes matter in blueberry and application provide reference.Blueberry, as the plant source of antibacterial substance, can be developed in a large number and be used.The domestic research to bacteriostatic active ingredients in blueberry is complete not enough.

The berry of Vaccinium shows certain fungistatic effect to food-borne pathogens.There are some researches show, the crude extract of whortle can suppress the growth of bacillus cereus, C.perfringens, helicobacter pylori and staphylococcus aureus consumingly, but does not produce inhibitory action to Lactobacillus rhamnosus.Cranberry crude extract can destroy Escherichia coli O 157: the adventitia of H7, Listeria monocytogenes, salmonella typhimurium and staphylococcus aureus, affects the eubolism of thalline and reach fungistatic effect.Short clump blueberry can suppress the normal growth of food-borne pathogens, then has protective effect to probio.Research finds that the blueberry of Vaccinium plant has inhibit activities effect to external gram-positive bacterium, negative bacteria and saccharomycete.Berries contains a lot of material composition useful to health, is the abundant source of a large amount of active component.Berries is rich in cellulose, vitamin and mineral matter, and especially wild berry is rich in aldehydes matter and organic acid etc., and these chemical compositions have good bacteriostatic activity.Aldehydes matter as blueberry, molka and rasp berry can suppress salmonella, and the phenols extract of blackcurrant, cowberry and Cranberry is to the growth of salmonella then unrestraint effect.Cranberry has potential fungistatic effect to staphylococcus aureus, and its fungistatic effect is obviously better than these small berries of strawberry, blackberry, blueberry and raspberry.Compare discovery, probio and blueberry can reduce the colitis caused by dextran sodium sulfate simultaneously, reduce enteric bacteria and transfer to mesenterium, enteric bacteria is reduced, and in enteron aisle, carboxylic acid, propionic acid amount raise.Blueberry shows the fungistatic effect to various thalline, is that what play a major role is aldehydes matter, organic acid, polysaccharide and anthocyan etc. owing to containing a large amount of bacteriostatic active ingredients in blueberry.Blueberry has the fungistatic effect of wide spectrum, can have inhibitory action to various bacteria.

Therefore, utilize blueberry broad-spectrum antibacterial to protect again the antagonistic property of probio, be combined with probio by blueberry, prepare blueberry fermented dairy product, beautifying face and moistering lotion effect is better compared with other deep processed product, is the first-selection of numerous blueberry deep processed product simultaneously.The people such as Wang Feifei with the blueberry of fresh high-quality, honey and milk for primary raw material, sucrose, stabilizing agent are auxiliary material, lactobacillus bulgaricus and streptococcus thermophilus are leavening, determine that the optimum formula of blueberry honey acidophilus milk is: in every pure cow's milk of 100mL, add 4% blueberry juice, 3% honey, 3% sucrose, fermentation 5h, starter culture 3%, fermentation temperature 42 ~ 43 DEG C, obtain the Yoghourt (Wang Feifei that structural state, fragrance and mouthfeel are all good, Xu Xianli, Tian Xiaolei etc. the technical study of blueberry honey acidophilus milk. processing of farm products, 2012 (1): 76-78.).The people such as Zhu Jianrong add blueberry jam development blueberry stirred yoghurt, the optimum process condition being determined agitating type blueberry Yoghourt by orthogonal test is: the proportioning of blueberry jam and milk is 1: 9, sucrose amount 6%, strain inoculation amount 3%, 43 DEG C of fermentation 6h (Zhu Jianrong, Hu Ying, Zhu Qiujing. the development of blueberry whisk yoghout. Guizhou animal and veterinary, 2012 (5): 57-59.).The people such as Su Wei have studied blueberry leben, determine that optimum material proportion is: cow's milk 60%, blueberry juice 14%, sucrose 6.5%, best fermentation parameter is inoculum concentration 3.2%, fermentation time 4.4h, fermentation temperature 42 DEG C of (Su Wei, letter element is flat, Xu Jing. the development of lactic fermentation type blueberry milk beverage. dairy industry science and technology, 2012,35 (1): 25-28.).The above-mentioned disclosed blueberry fermented dairy product shelf-life is short, viable bacteria content is low, poor stability, and blueberry activated material damage is larger.

Blueberry and acidified milk are organically combined and be processed into pressed candy, can be expected to overcome above-mentioned defect, Chinese patent CN103027176B discloses a kind ofly has pressed candy of beautifying face and moistering lotion effect and preparation method thereof, belongs to food processing field.The raw material composition of described pressed candy mainly comprises blueberry protoplasm, blackberry, blueberry magma, Cranberry magma, raspberry magma, frozen dried natto powder, distortion starch, resistant dextrin, vitamin C, natural flavouring and binding agent; First magma allocate by described preparation method, then with part material Homogeneous phase mixing, through high pressure homogenizer homogeneous, finally by spraying dry powder process, then mixes with part material, cross 200 mesh sieves, through rushing rotary tablet press more and get final product.Above-mentioned disclosed patent raw material is single, blueberry pulp preparation method tradition, and loss of effective components is large, and not containing probio, beautifying face and moistering lotion and antibacterial effect undesirable.Chinese patent CN103651789A discloses a kind of blueberry milkshake powder, be made up of white granulated sugar, sour milk powder, vegetable fat powder, blueberry powder, sucrose ester, sodium carboxymethylcellulose, Ipomoea batatas(L.)Lam, the percentage by weight of each component is as follows: white granulated sugar 35% ~ 45%, sour milk powder 25% ~ 30%, vegetable fat powder 15% ~ 25%, blueberry powder 8% ~ 10%, sucrose ester 0.8% ~ 1.2%, sodium carboxymethylcellulose 0.6% ~ 0.8%, Ipomoea batatas(L.)Lam 0.01% ~ 0.02%, through batching, mixing, microwave disinfection, packaging is made.Although above-mentioned disclosed patent is containing sour milk powder, not containing viable bacteria, blueberry powder and sour milk powder preparation method tradition, loss of effective components is large, and beautifying face and moistering lotion and antibacterial effect are still undesirable.Chinese patent CN103109930B discloses a kind of fruity probiotic yogurt sheet adding silk gum antifreeze peptide, its raw material is made up of following component by weight: skimmed milk power 10 ~ 14 parts, sucrose 2 ~ 5 parts, sericin peptide taken 1 ~ 3 part, 1 ~ 4 part, fruit material, the active probiotic 0.1 ~ 0.6 part of freeze-drying and dolomol 0.1 ~ 0.4 part, this invention additionally provides the preparation method of product, technical process comprises: the preparation of skimmed milk food-grade culture medium, the fermentation of probio, the interpolation of silk gum antifreeze peptide, the vacuum freeze drying of acidified milk, the techniques such as pressed powder, silk gum antifreeze peptide and the fruit jelly dry powder being rich in B family vitamin is with the addition of in probiotic yogurt sheet, not only make number of live bacteria of probiotics in sour milk tablet high, the bacterium vital preservation time is longer, and improve its taste flavor.Above-mentioned disclosed patent lactic acid bacteria or probiotics viable bacteria content still compared with low, fermented bacterium too large, the fruits and vegetables of tradition, functional poor, independent freeze-drying fruits and vegetables and Yoghourt energy resource consumption consuming time and Yoghourt preprocessing process heating power and shearing force cause nutriment to lose tradition processing that is large, pulp makes that Soluble Fiber cellulose content is lower, poor stability, shelf-life are short.

To sum up, blueberry is fermented together with cow's milk and is prepared into blueberry Yoghourt, be then lyophilized into powder again compressing tablet be expected to that obtained probiotics viable bacteria content is high, the improved body immunity of good stability, long shelf-life, there is the blueberry compressed tablet candy of beautifying face and moistering lotion and antibiotic effect, delaying sanility.

Summary of the invention

Technical problem solved by the invention is the defect overcoming existing blueberry compressed tablet candy, take blueberry as primary raw material, non-thermal processing method is adopted to be processed into blueberry pulp, with cow's milk, sucrose, Chinese herbal medicine extract, honey and stabilizing agent Homogeneous phase mixing, add leavening, adopt the mode of segmentation temperature-variable fermentation to ferment and be prepared into blueberry Yoghourt, probio is bred to greatest extent and there is good cold-and-heat resistent stress ability, then the protective agent added probio has a better anti-freezing effect is lyophilized into powder, obtain the high and functional blueberry Yoghourt freeze-dried powder that cold-and-heat resistent ability is strong of probiotics viable bacteria content, last and extractive of alfalfa, sprouted unpolished rice, plant extracts, dextrin, the prebiotic factor, the science such as dolomol are composite, through rushing rotary tablet press, to obtain a kind of probiotics viable bacteria content high more, good stability, the improved body immunity of long shelf-life, there is beautifying face and moistering lotion and antibiotic effect, the blueberry compressed tablet candy of delaying sanility.

In order to achieve the above object, the present invention is by the following technical solutions:

A blueberry compressed tablet candy for delaying sanility, the raw material primarily of following components by weight percent obtains:

Blueberry 85-95 part, cow's milk 50-60 part, sucrose 45-65 part; extractive of alfalfa 20-40 part, sprouted unpolished rice 20-30 part, Chinese herbal medicine extract 10-20 part; honey 10-18 part, plant extracts 8-10 part, leavening 7-9 part; dextrin 5-9 part, prebiotic factor 4-6 part, protective agent 3-5 part; fresh skim milk 1-3 part; 5% starch solution 0.5-1 part, dolomol 0.5-1 part, stabilizing agent 0.1-0.3 part.

Described cow's milk is fresh cow milk, and acidity is not more than 18 ° of T, and dry matter content is not less than 11.6%.

Further, described extractive of alfalfa is by composite with aloe science to clover higher for isoflavone content, soybean, onion, ginkgo etc., adopts the low temperature extractive techniques such as ultrasonic cleaning, freezing, pulverizing, high-pressure pulse electric extraction, ultrasonic wave added Microwave Extraction and obtains;

Preferably, the preparation method of described extractive of alfalfa, comprise the following steps: red clover, alfalfa, northeast soybean, onion, ginkgo, aloe are put into respectively the supersonic wave cleaning machine that 0.3-0.5% sodium bicarbonate solution is housed and clean 12-15min in 100-300W, 20-40KHz, drain, 10-15:5-7:3-5:1-3:1-3:1-2 Homogeneous phase mixing in mass ratio, then in-18--23 DEG C of freezing 5-10min; Be crushed to particle diameter 0.5-1.5mm immediately; To put in container and to add the benzinum of 10-15 times of weight, putting into water bath chader 35-45 DEG C of concussion 1-3h, suction filtration, drying to obtain mixed powder; Add mixed powder quality 15-25 60% ethanol doubly, be 3.5-5.5 by lactic acid adjust ph, at electric-field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, carries out high-pressure pulse electric process under pulse frequency 200-400Hz condition; Then in 30-40 DEG C of insulation 1-2h, be then warming up to 40-50 DEG C under power 150-300W condition, carry out microwave irradiation extraction 15-20min, under power 200-400W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Extracting liquid filtering, obtains filtrate, filter residue 1-3 times of weight 45-60 DEG C of water rinse 3 times, and rinsing liquid and filtrate merge, Homogeneous phase mixing, and being evaporated to solid content is more than 15%, and freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains extractive of alfalfa;

More preferably, described microwave irradiation is extracted as batch (-type) and extracts, i.e. microwave irradiation 10s, interval 20s;

In the extractive of alfalfa filtrate adopting said method to prepare, the extracted amount of isoflavones is 25.14-27.68mg/g, and crude extract yield is 38-40%, and crude extract purity is 4.41%.

Further, described sprouted unpolished rice take brown rice as raw material, through soaking, germinateing, the dry and a kind of functional stronger raw-food material of preparation, containing multiple enzyme system and functional, bioactivator;

Preferably, the preparation method of described sprouted unpolished rice, comprise the steps: brown rice to be placed on room temperature, power 100-300W, frequency 20-30KHz ultrasonic cleaning 5-10min in the supersonic wave cleaning machine that 0.1-0.3% sodium bicarbonate solution is housed, rinsing, to drain, put in 16-18 DEG C of water and soak 2-4h, simultaneously in electric-field intensity 20-40kV/cm, burst length 300-500 μ s, pulse frequency 100-300Hz carries out high-pressure pulse electric process, until brown rice moisture content is 35-38%; Brown rice after immersion drains, dark germination is carried out in growing floor, dark germination temperature keeps 23-25 DEG C, the dark germination time is 15-17h, and the aqueous solution of period sprinkling containing sodium ion 35-45mg/L, potassium ion 25-35mg/L, magnesium ion 12-18mg/L, zinc ion 12-15mg/L and calcium ion 8-10mg/L is until brown rice moisture content is to 43-45%; Stop spraying, in growing floor, proceed dark germination, dark germination temperature keeps 24-26 DEG C, and the dark germination time is 13-15h; Brown rice after germinateing again is dry in frequency 2450MHz, power 3000W, temperature 50-60 DEG C, thickness of feed layer 2-4cm condition microwave irradiation, and wherein microwave irradiation 10s, interval 10s, being dried to moisture is 12-14%, packs and obtains sprouted unpolished rice.

Further, described Chinese herbal medicine extract significantly improves lactic acid bacteria to resist the Chinese herbal medicine of hot and cold irritability, disease resistance and immunity to prepare for raw material;

Preferably, the preparation method of described Chinese herbal medicine extract, comprises the steps: to count by weight, takes Radix Astragali 15-17 part, motherwort 15-17 part, barrenwort 13-16 part, tuber of multiflower knotweed 12-15 part, dried orange peel 12-15 part, rhizoma zingiberis 8-12 part, Radix Codonopsis 6-10 part, seed of Chinese dodder 5-8 part; Put in the supersonic wave cleaning machine filling 0.1-0.3% sodium bicarbonate solution and clean 10-15min in 200W, 30KHz, drain, said herbal medicine being crushed to particle diameter is below 2mm, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixed material, control temperature 70-90 DEG C keeps 2-4h, then 40-50 DEG C is cooled to, be 3.5-4.5 by lactic acid adjust ph, Microwave Extraction 10-15min is carried out under power 150-300W condition, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Then the complex enzyme adding mixed material gross weight 0.5-1.5% carries out enzymolysis, be 5.5-6.8 by lactic acid adjust ph, enzymolysis 2-4h, finally add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1-3, control temperature keeps 3-4h to 60-78 DEG C, filters, obtains the first filtrate; Add the water of filter residue 1-3 times of weight, control temperature 85-95 DEG C keeps 1-3h, is then cooled to 25-35 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, Homogeneous phase mixing, being evaporated to solid content is more than 15%, and freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains Chinese herbal medicine extract;

Described complex enzyme is dextranase, zytase, pentosanase, pectase 2-4:2-4:1-3:1-3 Homogeneous phase mixing in mass ratio.

Further, described honey is one or more in blueberry nectar, clover honey, clover nectar, sophorae honey and honey of lychee flowers;

Preferably, described honey is blueberry nectar.

Further, described plant extracts is that the plant with remarkable beautifying face and moistering lotion effect is prepared for raw material;

Preferably, the preparation method of described plant extracts, comprises the steps: to count by weight, take pearl 20-40 part, root of Dahurain angelica 20-30 part, lavender 20-30 part, Radix Angelicae Sinensis 18-20 part, red sage root 12-15 part, rose 8-12 part, sophora flower 6-10 part, peach blossom 5-8 part, Chinese trumpet creeper 5-8 part, safflower 4-6 part; Put in the supersonic wave cleaning machine filling 0.1-0.3% sodium bicarbonate solution and clean 3-5min in 200W, 30KHz, drain, it is below 2mm that above-mentioned plant material is crushed to particle diameter, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtaining mixed material, control temperature 40-50 DEG C, be 3-4 by lactic acid adjust ph, under power 150-300W condition, carry out Microwave Extraction 20-40min, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Then the mixed enzyme adding mixed material gross weight 0.5-1.5% carries out enzymolysis, be 5.5-6.8 by lactic acid adjust ph, enzymolysis 2-4h, finally add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1-3, control temperature keeps 3-4h to 60-70 DEG C, filters, obtains the first filtrate; Add the water of filter residue 1-3 times of weight, control temperature 85-95 DEG C keeps 1-2h, is then cooled to 25-35 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 3-5:1-3, Homogeneous phase mixing, being evaporated to solid content is more than 15%, and freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains plant extracts;

Described mixed enzyme is dextranase, protease, zytase, pentosanase, pectase 4-6:4-6:2-4:1-3:1-3 Homogeneous phase mixing in mass ratio.

Further, described leavening comprise in following probio pulvis any one or multiple: such as: Lactobacillus plantarum (Lactobacillusplantarum), bifidobacterium bifidum (Bifidobacteriumbifidum), bifidobacterium infantis (B.infantis), bifidobacterium longum (B.longum), bifidobacterium breve (B.breve), bifidobacterium adolescentis (B.adolescentis), bifidobacterium lactis (Bifidobacteriumlactis), lactobacillus bulgaricus (Lactobacillus.Bulgaricus), lactobacillus acidophilus (L.acidophilus), Lactobacillus casei (Lactobacilluscasei), Lactobacillus rhamnosus (Lactobacillusrhamnosus), streptococcus thermophilus (Streptococcusthermophilus),

Preferably, described leavening is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 25-35 part, bifidobacterium bifidum 20-25 part, bifidobacterium lactis 20-23 part, streptococcus thermophilus 20-23 part, bifidobacterium longum 16-18 part, bifidobacterium infantis 16-18 part, bifidobacterium adolescentis 15-17 part, lactobacillus bulgaricus 13-15 part, lactobacillus acidophilus 12-14 part, Lactobacillus casei 8-10 part, Lactobacillus rhamnosus 6-8 part;

Preferably, described Lactobacillus plantarum pulvis is that the bacterium that sets out is prepared according to a conventional method with Lactobacillus plantarum (Lactobacillusplantarum) QZ-5 disclosed in Chinese patent CN101974467A, and it is CGMCCNo.4200 at the deposit number at China Committee for Culture Collection of Microorganisms's common micro-organisms center.This bacterial classification can produce multiple pathogen and the inhibited bacteriocin of spoilage organisms, and this bacteriocin has good heat endurance and absolute acid stability, and to protease-sensitive, antimicrobial spectrum is wide;

Preferably, described Lactobacillus plantarum pulvis is that the bacterium that sets out is prepared according to a conventional method with Lactobacillus plantarum (Lactobacillusplantarum) FM-LP1-1 disclosed in Chinese patent CN104531578A, and it is CGMCCNO.9488 at the deposit number at China Committee for Culture Collection of Microorganisms's common micro-organisms center.This bacterial strain not only has effectively degrading nitrite ability, strong acid producing ability, higher bile tolerance ability, and this bacterium has excellent fermenting property and resistance to osmotic pressure performance, containing 200mg/LNaN0 ₂mRS nutrient solution in, the cell density inoculating this bacterium is 1.01 × 10 ⁷-1.54 × 10 ⁷cfu/ml, cultivates 24h, NaN0 under 25 DEG C of conditions ₂degradation rate reach more than 97.5%, cultivate 48h, NaN0 ₂degradation rate reach more than 99.1%; In MRS nutrient solution, cultivate 24h, the pH value of nutrient solution reaches 3.51;

More preferably, described Lactobacillus plantarum pulvis is that the pulvis prepared according to a conventional method by Lactobacillus plantarum CGMCCNo.4200 and Lactobacillus plantarum CGMCCNO.9488 mixes with arbitrary proportion;

More preferably, described Lactobacillus plantarum pulvis is the pulvis 6-8:3-5 Homogeneous phase mixing in mass ratio prepared according to a conventional method by Lactobacillus plantarum CGMCCNo.4200 and Lactobacillus plantarum CGMCCNO.9488.

Further, described dextrin is any one or the two kinds of mixing in maltodextrin, resistant dextrin;

Preferably, described dextrin is maltodextrin, resistant dextrin 7-8:1-2 Homogeneous phase mixing in mass ratio.

Further, described prebiotic factor weight consists of: oligoisomaltose 10-15 part, galactooligosaccharide 10-15 part, FOS 8-10 part, xylo-oligosaccharide 8-10 part, stachyose 6-8 part, soyabean oligosaccharides 6-8 part, gossypose 6-8 part, oligomeric maltose 5-7 part.

Further, described protective agent is for raw material with the animal or plant containing antifreeze protein, obtained through high-pressure pulse electric extraction, ultrasonic assistant Microwave Extraction and compound enzyme enzymolysis, effectively can improve lactic acid bacteria in blueberry Yoghourt at freezing dry process Lactobacillus Survival, significantly improve the viable bacteria content of blueberry sour milk powder;

Preferably, described protectant preparation method, comprise the steps: winter rye, Ammopiptanthus mongolicus, Rhodiola algida Var. tangutica leaf, sharkskin collagen cleans respectively, drain, 8-10:4-6:3-5:2-4 Homogeneous phase mixing in mass ratio, adding mixed material quality 0.1-1 pH value is doubly that the lactic acid of 3.8-4.5 soaks 3-8h, pulverize immediately after-18--22 DEG C of freezing 1-2h, freezing thickness of feed layer 3-5cm, crushed material particle diameter 0.5-3mm, then crushed material quality 10-20 water is doubly added, be 3.5-5.5 by lactic acid adjust ph, at electric-field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, high-pressure pulse electric process 20-30min is carried out under pulse frequency 200-300Hz condition, then under power 150-300W condition, carry out microwave irradiation in room temperature and extract 15-20min, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously, add the compound enzyme of extract quality 3-5%, in 45-55 DEG C of enzymolysis 30-50min, enzymolysis liquid filters, filtrate is concentrated, low-temperature grinding to particle diameter is that namely 0.1-0.3mm obtains protective agent,

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 3-5:3-5:2-4:2-4:1-3 Homogeneous phase mixing in mass ratio;

More preferably, described microwave irradiation is extracted as batch (-type) and extracts, i.e. microwave irradiation 10s, interval 20s.

Further, described stabilizing agent is that any one or a few in modified dietary fiber, tremella polysaccharides, gelatin, pectin mixes with arbitrary proportion, quality is thin, layering and whey separate out phenomenon can effectively to prevent Yoghourt from occurring, blueberry Yoghourt quality, state and mouthfeel finally can be made homogeneous, stable, fine and smooth;

Preferably, described stabilizing agent is formed by the raw material Homogeneous phase mixing of following parts by weight: modified dietary fiber 16-20 part, tremella polysaccharides 12-15 part, gelatin 1-3 part, pectin 1-3 part;

Described modified dietary fiber is that the Soluble Fiber cellulose content obtained through physics, chemistry or biological method process by dietary fiber is high, biologically active strong, has cellulose that is important, positive role to human body beneficial flora, compared with full diet fiber, its biological action is more powerful;

Preferably, described modified fibre is obtained through biology enzyme enzymolysis by one or more in inulin, apple fiber, Semen Tritici aestivi fiber, common oats fibre;

More preferably, the preparation method of described modified dietary fiber, comprise the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber 4-6:3-5:2-4 Homogeneous phase mixing in mass ratio, add its quality 3-7 water doubly, room temperature 200-300W, 35-40KHz condition ultrasonic extraction 10-15min, then at electric-field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric process 10-15min under pulse frequency 200-300Hz condition; Be 4.5-6.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains modified dietary fiber;

Described biology enzyme is cellulase, zytase, laccase, pectase, tannase 2-4:1-3:1-3:1-2:1-2 Homogeneous phase mixing in mass ratio.

Further, described fresh non-fat milk solid content >=10%.

Further, in described starch solution, starch is one or more in the soluble starches such as cornstarch, farina, rice starch, little rice starch;

Preferably, described starch is cornstarch;

More preferably, described starch is waxy corn starch.

Another object of the present invention is to provide the preparation method of the blueberry compressed tablet candy of above-mentioned delaying sanility, comprises the steps:

1) preparation of blueberry pulp: take blueberry by formula, blueberry is put into the supersonic wave cleaning machine filling 0.1-0.3% sodium bicarbonate solution, 200-400W under room temperature, 35-45KHz cleans 5-10min, drain, broken, in electric-field intensity 30-40kV/cm under room temperature, burst length 600-800 μ s, pulse frequency 200-400Hz high-pressure pulse electric process 5-10min, glue grinds, outlet pulp particle diameter is made to reach 150-250 μm, then under room temperature in electric-field intensity 20-30kV/cm, burst length 400-600 μ s, pulse frequency 200-400Hz high-pressure pulse electric process 3-5min, vacuum outgas obtains former pulp, add cellulase, pectase, tannase and protease wherein, its content is made to be respectively 80-100ppm, 50-70ppm, 50-60ppm and 25-35ppm, in 45-55 DEG C of enzymolysis 1-3h, be warming up to 85-95 DEG C of insulation 3-5min, be cooled to room temperature immediately, 80-100 eye mesh screen filters, and namely filtrate reduced in volume obtains blueberry pulp to solid content to 30-40%,

2) preparation of blueberry Yoghourt: cow's milk after clean breast under room temperature in electric-field intensity 30-40kV/cm, burst length 400-600 μ s, pulse frequency 300-500Hz carries out high-pressure pulse electric sterilization 10-20min, with sucrose, Chinese herbal medicine extract, honey, stabilizing agent and step 1) the blueberry pulp Homogeneous phase mixing that obtains, at 55-65 DEG C after abundant stirring and dissolving, homogeneous under 18-22MPa, then 32-35 DEG C is cooled in electric-field intensity 20-30kV/cm, burst length 200-400 μ s, pulse frequency 200-400Hz carries out high-pressure pulse electric sterilization 5-10min, adjusted to ph is 6.0-7.0, add the 30-40% ferment at constant temperature 0.5-1h of leavening quality, then with the ramp of 0.8-1.0 DEG C/min to 40-45 DEG C, add the 60-70% ferment at constant temperature 2-4h of leavening quality, last with the ramp of 0.6-0.8 DEG C/min to 50-55 DEG C, continuing fermentation to Yoghourt pH value is that 3.5-4.5 stops fermenting and obtaining blueberry Yoghourt,

3) preparation of blueberry sour milk powder: be that the starch solution of 5% is respectively at 121 DEG C of sterilizing 8-10min by fresh skim milk and mass ratio, be cooled to 45-55 DEG C immediately, then with protective agent Homogeneous phase mixing, step 2 is added after abundant dissolving) in the blueberry Yoghourt that obtains, namely freeze drying obtains blueberry sour milk powder according to a conventional method;

4) compressing tablet: sprouted unpolished rice is crushed to particle diameter 0.1-0.3mm in 40-60 DEG C, with extractive of alfalfa, plant extracts, dextrin, the prebiotic factor, dolomol and step 3) the blueberry sour milk powder Homogeneous phase mixing that obtains, then 50-100 mesh sieve is crossed, making pressed candy through rushing rotary tablet machine more, obtaining the blueberry compressed tablet candy of delaying sanility.

Blueberry Yoghourt viable lactic acid bacteria content prepared by said method is 1.12 × 10 ¹²-1.36 × 10 ¹²cFU/ml, the viable bacteria content of blueberry sour milk powder is 8.65 × 10 ¹¹-9.78 × 10 ¹¹cFU/g, the viable bacteria content of blueberry compressed tablet candy is 8.22 × 10 ¹¹-9.49 × 10 ¹¹cFU/g, under normal temperature, shelf-life 30-36 month.

Beneficial effect:

The present invention take blueberry as primary raw material, non-thermal processing method is adopted to be processed into blueberry pulp, infect to prevent process miscellaneous bacteria, improve the content of the soluble cellulose of juice yield and increase pulp, prevent pulp brown stain, remain the anthocyanidin in blueberry to greatest extent, vitamin C, vitamin E, the content of the functionality active component such as trace element and dietary fiber, the synthesis of collagen needs vitamin C to participate in, and collagen determines the elasticity of skin, vitamin C can protect other antioxidant contents, as vitamin E, oxidizing ferment, anthocyanidin, prevent the injury of radical pair human body.Anthocyanidin can kill the bacterium be attached in gastric mucosa and urinary system, can prevent stomach trouble and gynecological disease; Free radical can cause the oxidative damage of fat, protein and nucleic acid, anthocyanidin energy scavenging free radicals, thus protected protein is not oxidized.The combination of anthocyanidin and collagen and hard elastics albumen, makes the cell membrane of mast cell and basophil to be formed the oxidation resistant protective layer of one deck, plays the effect of reparation and Cell protection.With cow's milk, sucrose, Chinese herbal medicine extract, honey and stabilizing agent Homogeneous phase mixing, add containing producing the Lactobacillus plantarum CGMCCNo.4200 of multiple pathogen and the inhibited bacteriocin of spoilage organisms and the leavening of Lactobacillus plantarum CGMCCNO.9488 with effectively degrading nitrite ability, strong acid producing ability, higher bile tolerance ability, the propagation of lactic acid bacteria in enteron aisle, field planting and detoxication and toxicant eliminating function ability can be significantly improved, effectively improve body immunity and gastrointestinal function, probiotic relative to existing leavening, functional stronger.Adopt the mode of segmentation temperature-variable fermentation to ferment and be prepared into blueberry Yoghourt, high-pressure pulse electric non-thermal sterilization technology is adopted to instead of traditional pasteurize in Yoghourt preparation process, remain the heat-sensitive nutrition in fresh milk to greatest extent, effectively prevent the living contaminants of process, stabilize and increase local flavor and the mouthfeel of Yoghourt, save the energy, there is the irritable Chinese herbal medicine extract of cold-and-heat resistent, can effectively prevent Yoghourt from occurring quality is thin, layering and whey are separated out stabilizing agent, alternating temperature Yoghourt fermentation technique can make lactic acid bacteria breed to reach the cold-and-heat resistent stress ability of maximizations, raising lactic acid bacteria, can obtain that delicate mouthfeel, quality are homogeneous, in stable condition, viable bacteria content much higher function blueberry Yoghourt, then the protective agent added probio has a better anti-freezing effect is lyophilized into powder, obtain the high and functional blueberry Yoghourt freeze-dried powder that cold-and-heat resistent ability is strong of probiotics viable bacteria content, last and extractive of alfalfa, sprouted unpolished rice, plant extracts, dextrin, the prebiotic factor, the science such as dolomol are composite, through rushing rotary tablet press, to obtain a kind of probiotics viable bacteria content high more, good stability, the improved body immunity of long shelf-life, there is beautifying face and moistering lotion and antibiotic effect, the blueberry compressed tablet candy (concrete test effect is shown in embodiment 5-12) of delaying sanility.The viable bacteria content of blueberry compressed tablet candy is 8.22 × 10 ¹¹-9.49 × 10 ¹¹cFU/g, under normal temperature, shelf-life 30-36 month.Concrete know-why is as follows:

1. extractive of alfalfa of the present invention is by clover higher for isoflavone content, soybean, onion, ginkgo etc. are composite with aloe science, adopt ultrasonic cleaning, freezing, pulverize, high-pressure pulse electric extracts, low temperature extractive techniques such as ultrasonic wave added Microwave Extraction and obtaining, remain the content of isoflavones in raw material to greatest extent, and simultaneously containing clover isoflavones, isoflavones, the multiple isoflavones such as onion isoflavones, gained isoflavones recovery rate is high, purity is higher, physiologically active is stronger, composite with aloe (gel) science, enhance the stability of isoflavones, reduce preparation, store and use procedure in loss, cooperative effect is stronger, in the extractive of alfalfa filtrate adopting said method to prepare, the extracted amount of isoflavones is 25.14-27.68mg/g, and crude extract yield is 38-40%, and crude extract purity is 4.41%, the extractive of alfalfa of preparation can significantly improve and balance women's serum estrogen level, effective self endocrine metabolic diseases of promotion women and woman organ (as ovary, fallopian tubal) absorption of nutrient ingredients, promote the absorption to calcium, reduce calcium loss, powerful abatement free radical, improves immunity of organisms, final enhancing body immunity and delaying senility, reaches beautifying face and moistering lotion and antibacterial effect.Reduce residues of pesticides, heavy metal ion and the worm's ovum etc. introduced because of raw material simultaneously, substantially increase the foodsafety of raw material.

2. plant extracts of the present invention is to have the flower of remarkable beautifying face and moistering lotion effect, grass, Chinese herbal medicine and pearl science composite, breach conventional boil, the eating method of grinding, through ultrasonic cleaning, low temperature enzymolysis, prepared by water extraction alcohol extracting and ultrasonic assistant Microwave Extraction, extract active constituent content is high, lose low, raw material availability is high, cost is low, composite rear cosmetic result is good, synergy is strong, can liver and nourishing stomach by force, promoting blood circulation for regulating menstruation, relax bowel, resolving stagnation for tranquilization, can chill out again, balance endocrine, to enrich blood gas, also can to releive mood, anti-inflammation and sterilization, dispelling fatigue, improve physique, effect of moist skin, final adjustable endocrine, heighten immunity, delay senility and beautifying face and moistering lotion and suitable for men women and children, reduce the residues of pesticides introduced because of raw material simultaneously, heavy metal ion and worm's ovum etc., substantially increase the foodsafety of raw material.

3. the composite various lactobacillus agent of leavening science of the present invention, kind is many, function is complete, by force probiotic, particularly Lactobacillus plantarum CGMCCNo.4200 can produce multiple pathogen and the inhibited bacteriocin of spoilage organisms, and this bacteriocin has good heat endurance and absolute acid stability, and to protease-sensitive, antimicrobial spectrum is wide; Lactobacillus plantarum CGMCCNO.9488 not only has effectively degrading nitrite ability, strong acid producing ability, higher bile tolerance ability, and this bacterium has excellent fermenting property and resistance to osmotic pressure performance; Relative to existing ferment agent for sour milk fermenting power, probiotic, functional stronger.

4. stabilizing agent of the present invention is through concentrating on studies for many years, form by a large amount of scientific experiments is composite, not containing the chemical addition agent with any toxic and side effect, any potential harm can not be produced to human body, without addition restriction, be mainly natural plant raw material, foodsafety is strong, effective, quality is thin, layering and whey separate out phenomenon can effectively to prevent Yoghourt from occurring, blueberry compressed tablet candy quality, state and mouthfeel finally can be made homogeneous, stable, fine and smooth.Ultrasonic extraction, high-pressure pulse electric extract and biological enzymolysis combination of sciences by modified dietary fiber wherein, gained modified dietary fiber Soluble Fiber cellulose content is high, more easily utilized by probio, improve probio in the growth of human body intestinal canal and fertility, increase kind and the quantity of probio flora, reduce human body intestinal canal pH value, improve human body intestinal canal micro-ecological environment; High adsorption capacity, through modified, cellulosic specific area increases, and network is enriched, and absorption affinity strengthens, and the organic molecule ability of chelating, absorption cholesterol and bile acids is stronger, suppress human body to their absorption; Ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is stronger, effectively prevent body weight for humans metal poisoning; The stronger and impact of acid and alkali, alkali, salt of retentiveness, dilatancy, thickening property.Regulate and maintain the resident time of gut flora, strengthen the absorption and digestion ability of enteron aisle, improve immunity of organisms; Effective promotion gastrointestinal peristalsis, slows down and eliminates the bad reaction such as gasteremphraxis, abdominal distension; Powerful bury function can prevent environment (oxygen, temperature, illumination, water activity etc.) factor from, on the impact of product quality, stabilizing the biologically active of pressed candy, extending the shelf-life of pressed candy.

5. protective agent of the present invention with the animal or plant containing antifreeze protein for raw material, obtained through high-pressure pulse electric extraction, ultrasonic assistant Microwave Extraction and biological enzymolysis, loss of effective components is few, recovery rate is high, successful, effectively can improve lactic acid bacteria in blueberry Yoghourt at freezing dry process Lactobacillus Survival, significantly improve the viable bacteria content of blueberry compressed tablet candy; And raw material is single, composition is simple, most importantly not containing chemical substance, foodsafety is strong, with skim milk and starch solution science composite, effect is better.

6. sprouted unpolished rice of the present invention contains abundant bioactivator and functional materials, can be the nutriment that lactic acid bacteria provides abundant, the multiple enzyme system (protease, amylase, cellulase, lipase, phosphate etc.) wherein contained on the one hand can by the macromolecular substances of self: starch, protein, fat, cellulose change small-molecule substance into; Sprouted unpolished rice germination percentage reaches more than 98%, the long 0.4-0.6mm of bud, and length is homogeneous, sprout damage is low is 1-2%, and functional materials content is high, and wherein alpha-aminobutyric acid content is 305.8-316.6mg/100g, glutathione 17.6-18.8mg/100g, IP6 (IP ₆) 459.7-491.6mg/100g, dietary fiber 3.5-3.8mg/100g, add functional, stability and the palatability of Yoghourt.

7. the Chinese herbal medicine extract science prepared of the present invention is composite significantly improves lactic acid bacteria and resists cold, heat stress, the Chinese herbal medicine of disease resistance and immunity is raw material, through ultrasonic cleaning, low temperature enzymolysis, prepared by water extraction alcohol extracting and ultrasonic assistant Microwave Extraction, extract active constituent content is high, cost is low, effectively can strengthen the ability of the anti-low temperature of lactic acid bacteria and high temperature, adaptive temperature changing environment, for adaptation temperature-variable fermentation and freeze drying environment have established solid foundation, effective raising pressed candy viable bacteria content, simultaneously can lactic acid bacteria inhibiting after fermentation, prevent Yoghourt peracid from coordinating to keep yoghourt-flavored and mouthfeel, also can prevent pressed candy miscellaneous bacteria from encroaching on, extend the shelf-life of product.

8. blueberry pulp preparation process of the present invention adopts ultrasonic cleaning, effectively can reduce the introducing of process residues of pesticides and heavy metal ion, improves the foodsafety of fruit juice; Under fruit juice room temperature after broken and glue grind, after high-pressure pulse electric process, its quality is more natural, and high, the blunt enzyme of rate of squeezing the juice is effective, miscellaneous bacteria content is extremely low, is significantly better than the quality index of commodity fruit juice; Adopt degasification technique, effectively reduce the dissolved oxygen content in juice production process, effectively improve the liquid phase stability of fruit juice; Glue mill and biological enzymolysis are organically combined, the complex enzyme zymohydrolysis of the fruit juice within the scope of certain particle size after science is composite, pigment of fruit juice tack more by force, stripping quantity is larger, dispersion force liquid phase that is stronger, pigment is more stable; By multiple non-thermal technology combine with technique process fruit juice, effectively prevent process miscellaneous bacteria from infecting, improve the content of the soluble cellulose of juice yield and increase pulp, prevent pulp brown stain, keep fruit juice natural quality.

9. the prebiotic factor of the present invention can be lactic acid bacteria and provides abundant nutriment, is conducive to probio in the propagation of enteron aisle and field planting, suppresses harmful bacteria growth; The membership that adds of resistant dextrin promotes that small intestine is to the absorption of amino acid, mineral matter and anthocyanidin, and composite with maltodextrin science, effect is more remarkable.

10. the preparation method of blueberry compressed tablet candy of the present invention adopts non-thermal technology technology; operating efficiency is high; energy resource consumption is few; environmentally friendly, pollution-free, decrease the introducing of food additives and chemical assistant; effectively improve foodsafety; improve product quality and reduce production cost, achieve low-carbon (LC) productive target, can scale, industrialized development.Omnidistance employing low temperature processing techniques, optimize fermented bacterium and technique, with the addition of functional raw material, the pressed candy delicate mouthfeel of preparation, quality is homogeneous, in stable condition, number of viable is high, nutritious, quality is natural, by force functional, long shelf-life, biologically active is high, mouthfeel and local flavor and appetite strong, foodsafety is high, body immunity can be improved, resistance against diseases, endurance and anti-fatigue ability, improve functions of intestines and stomach, it should be noted that the eating effect of this blueberry compressed tablet candy is that each component is worked in coordination with mutually, interactional result, the not superposition of simple raw material function, the science of each raw material components is composite and extract, the effect produced is considerably beyond the superposition of each single component function and effect, there is advanced and practicality preferably.

Detailed description of the invention

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.

Prepared by embodiment 1 raw material

1. the preparation of extractive of alfalfa:

The preparation method of described extractive of alfalfa, comprise the following steps: red clover, alfalfa, northeast soybean, onion, ginkgo, aloe are put into respectively the supersonic wave cleaning machine that 0.4% sodium bicarbonate solution is housed and clean 14min in 200W, 30KHz, drain, 12:6:4:2:2:1.5 Homogeneous phase mixing in mass ratio, then in-20 DEG C of freezing 8min; Be crushed to particle diameter 1.0mm immediately; To put in container and to add the benzinum of 12 times of weight, putting into water bath chader 40 DEG C concussion 2h, suction filtration, drying to obtain mixed powder; Adding 60% ethanol of mixed powder quality 20 times, is 4.5 by lactic acid adjust ph, and at electric-field intensity 30kV/cm under room temperature, burst length 400 μ s, carries out high-pressure pulse electric process under pulse frequency 300Hz condition; Then in 35 DEG C of insulation 1.5h, be then warming up to 45 DEG C under power 200W condition, carry out microwave irradiation extraction 18min, wherein, microwave irradiation 10s, interval 20s; Under power 300W, frequency 35KHz condition, carry out ultrasonic assistant extraction simultaneously; Extracting liquid filtering, obtains filtrate, filter residue 2 times of weight, 52 DEG C of water rinses 3 times, and rinsing liquid and filtrate merge, Homogeneous phase mixing, and being evaporated to solid content is more than 15%, and freeze drying, low-temperature grinding to particle diameter are that namely 0.2mm obtains extractive of alfalfa;

In the extractive of alfalfa filtrate adopting said method to prepare, the extracted amount of isoflavones is 27.68mg/g, and crude extract yield is 40%, and crude extract purity is 4.41%.

2. the preparation of plant extracts:

The preparation method of described plant extracts, comprises the steps: to count by weight, takes pearl 30 parts, the root of Dahurain angelica 25 parts, lavender 25 parts, Radix Angelicae Sinensis 19 parts, the red sage root 13 parts, rose 10 parts, 8 parts, sophora flower, 6 parts, peach blossom, Chinese trumpet creeper 6 parts, 5 parts, safflower; Put in the supersonic wave cleaning machine filling 0.4% sodium bicarbonate solution and clean 4min in 200W, 30KHz, drain, it is below 2mm that above-mentioned plant material is crushed to particle diameter, put Homogeneous phase mixing in container and add the water of 5 times of weight, obtaining mixed material, control temperature 45 DEG C, be 3.5 by lactic acid adjust ph, under power 200W condition, carry out Microwave Extraction 30min, under power 250W, frequency 35KHz condition, carry out ultrasonic assistant extraction simultaneously; Then the mixed enzyme adding mixed material gross weight 1.0% carries out enzymolysis, be 5.2 by lactic acid adjust ph, enzymolysis 3h, finally add the mixture of mixed material 2 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:2, control temperature to 65 DEG C keeps 3.5h, filters, obtains the first filtrate; Add the water of filter residue 2 times of weight, control temperature 90 DEG C keeps 1.5h, is then cooled to 30 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 4:2, Homogeneous phase mixing, being evaporated to solid content is more than 15%, and freeze drying, low-temperature grinding to particle diameter are that namely 0.2mm obtains plant extracts;

Described mixed enzyme is dextranase, protease, zytase, pentosanase, pectase 5:5:3:2:2 Homogeneous phase mixing in mass ratio.

3. the preparation of sprouted unpolished rice:

The preparation method of described sprouted unpolished rice, comprise the steps: brown rice to be placed on room temperature, power 200W, frequency 25KHz ultrasonic cleaning 8min in the supersonic wave cleaning machine that 0.2% sodium bicarbonate solution is housed, rinsing, to drain, put in 17 DEG C of water and soak 3h, simultaneously in electric-field intensity 30kV/cm, burst length 400 μ s, pulse frequency 200Hz carries out high-pressure pulse electric process, until brown rice moisture content is 36%; Brown rice after immersion drains, dark germination is carried out in growing floor, dark germination temperature keeps 24 DEG C, the dark germination time is 16h, and the aqueous solution of period sprinkling containing sodium ion 40mg/L, potassium ion 30mg/L, magnesium ion 15mg/L, zinc ion 13mg/L and calcium ion 9mg/L is until brown rice moisture content to 44%; Stop spraying, in growing floor, proceed dark germination, dark germination temperature keeps 25 DEG C, and the dark germination time is 14h; Brown rice after germinateing again is in frequency 2450MHz, power 3000W, temperature 55 DEG C, the drying of thickness of feed layer 3cm condition microwave irradiation, and wherein microwave irradiation 10s, interval 10s, being dried to moisture is 13%, packs and obtains sprouted unpolished rice.

4. the preparation of Chinese herbal medicine extract:

The preparation method of described Chinese herbal medicine extract, comprises the steps: to count by weight, takes the Radix Astragali 16 parts, motherwort 16 parts, barrenwort 15 parts, the tuber of multiflower knotweed 13 parts, dried orange peel 13 parts, rhizoma zingiberis 10 parts, Radix Codonopsis 8 parts, the seed of Chinese dodder 6 parts; Put in the supersonic wave cleaning machine filling 0.2% sodium bicarbonate solution and clean 12min in 200W, 30KHz, drain, said herbal medicine being crushed to particle diameter is below 2mm, puts Homogeneous phase mixing in container and adds the water of 5 times of weight, obtaining mixed material, control temperature 80 DEG C keeps 3h, then being cooled to 45 DEG C, is 4.0 by lactic acid adjust ph, under power 200W condition, carry out Microwave Extraction 12min, under power 250W, frequency 35KHz condition, carry out ultrasonic assistant extraction simultaneously; Then the complex enzyme adding mixed material gross weight 1.0% carries out enzymolysis, be 5.2 by lactic acid adjust ph, enzymolysis 3h, finally add the mixture of mixed material 2 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:2, control temperature to 70 DEG C keeps 3.5h, filters, obtains the first filtrate; Add the water of filter residue 2 times of weight, control temperature 90 DEG C keeps 2h, is then cooled to 30 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 3:2, Homogeneous phase mixing, being evaporated to solid content is more than 15%, and freeze drying, low-temperature grinding to particle diameter are that namely 0.2mm obtains Chinese herbal medicine extract;

Described complex enzyme is dextranase, zytase, pentosanase, pectase 3:3:2:2 Homogeneous phase mixing in mass ratio.

5. protectant preparation:

Described protectant preparation method, comprise the steps: winter rye, Ammopiptanthus mongolicus, Rhodiola algida Var. tangutica leaf, sharkskin collagen cleans respectively, drain, 9:5:4:3 Homogeneous phase mixing in mass ratio, the pH value adding mixed material quality 0.5 times be 4.1 lactic acid soak 5h, pulverize immediately after-20 DEG C of freezing 1.5h, freezing thickness of feed layer 4cm, crushed material particle diameter 1.2mm, then the water of crushed material quality 15 times is added, be 4.5 by lactic acid adjust ph, at electric-field intensity 30kV/cm under room temperature, burst length 400 μ s, high-pressure pulse electric process 25min is carried out under pulse frequency 250Hz condition, then under power 200W condition, carry out microwave irradiation in room temperature and extract 18min, wherein, microwave irradiation 10s, interval 20s, carry out ultrasonic assistant extraction simultaneously under power 250W, frequency 35KHz condition, add the compound enzyme of extract quality 4%, in 50 DEG C of enzymolysis 40min, enzymolysis liquid filters, filtrate is concentrated, low-temperature grinding to particle diameter is that namely 0.2mm obtains protective agent,

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 4:4:3:3:2 Homogeneous phase mixing in mass ratio.

6. the preparation of modified dietary fiber:

The preparation method of described modified dietary fiber, comprise the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber 5:4:3 Homogeneous phase mixing in mass ratio, add the water of its quality 5 times, room temperature 250W, 35KHz condition ultrasonic extraction 12min, then at electric-field intensity 30kV/cm, burst length 500 μ s, carries out high-pressure pulse electric process 12min under pulse frequency 300Hz condition; Be 5.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.2%, in 50 DEG C of enzymolysis 35min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to particle diameter are that namely 0.2mm obtains modified dietary fiber;

Described biology enzyme is cellulase, zytase, laccase, pectase, tannase 3:2:2:1.5:1.5 Homogeneous phase mixing in mass ratio.

The extractive of alfalfa that following examples 2-4 uses, plant extracts, sprouted unpolished rice, Chinese herbal medicine extract, protective agent, modified dietary fiber are embodiment 1 to be prepared, and other is commercial commodity.

Embodiment 2

Blueberry 90 parts, 55 parts, cow's milk, sucrose 55 parts; extractive of alfalfa 30 parts, sprouted unpolished rice 25 parts, Chinese herbal medicine extract 15 parts; blueberry nectar 14 parts, plant extracts 9 parts, leavening 8 parts; 7 parts, dextrin, the prebiotic factor 5 parts, protective agent 4 parts; fresh skim milk 2 parts; 5% waxy corn starch solution 0.8 part, dolomol 0.8 part, stabilizing agent 0.2 part.

Described leavening is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 30 parts, bifidobacterium bifidum 22 parts, bifidobacterium lactis 21 parts, streptococcus thermophilus 21 parts, bifidobacterium longum 17 parts, bifidobacterium infantis 17 parts, bifidobacterium adolescentis 16 parts, lactobacillus bulgaricus 14 parts, lactobacillus acidophilus 13 parts, Lactobacillus casei 9 parts, Lactobacillus rhamnosus 7 parts;

Described Lactobacillus plantarum pulvis is the pulvis 7:4 Homogeneous phase mixing in mass ratio prepared according to a conventional method by Lactobacillus plantarum CGMCCNo.4200 and Lactobacillus plantarum CGMCCNO.9488.

Described dextrin is maltodextrin, resistant dextrin 7.5:1.5 Homogeneous phase mixing in mass ratio.

Described prebiotic factor weight consists of: oligoisomaltose 12 parts, galactooligosaccharide 12 parts, FOS 9 parts, xylo-oligosaccharide 9 parts, stachyose 7 parts, soyabean oligosaccharides 7 parts, gossypose 7 parts, oligomeric maltose 6 parts.

Described stabilizing agent is formed by the raw material Homogeneous phase mixing of following parts by weight: modified dietary fiber 18 parts, tremella polysaccharides 13 parts, 2 parts, gelatin, pectin 2 parts.

Preparation method, comprises the steps:

1) preparation of blueberry pulp: take blueberry by formula, blueberry is put into the supersonic wave cleaning machine filling 0.1-0.3% sodium bicarbonate solution, under room temperature, 300W, 40KHz clean 8min, drain, broken, in electric-field intensity 35kV/cm, burst length 700 μ s, pulse frequency 300Hz high-pressure pulse electric process 8min under room temperature, glue grinds, outlet pulp particle diameter is made to reach 200 μm, then under room temperature in electric-field intensity 25kV/cm, burst length 500 μ s, pulse frequency 300Hz high-pressure pulse electric process 4min, vacuum outgas obtains former pulp; Add cellulase, pectase, tannase and protease wherein, its content is made to be respectively 90ppm, 60ppm, 55ppm and 30ppm, in 50 DEG C of enzymolysis 2h, be warming up to 90 DEG C of insulation 4min, be cooled to room temperature immediately, 90 eye mesh screens filter, and namely filtrate reduced in volume obtains blueberry pulp to solid content to 35%;

2) preparation of blueberry Yoghourt: cow's milk after clean breast under room temperature in electric-field intensity 35kV/cm, burst length 500 μ s, pulse frequency 400Hz carries out high-pressure pulse electric sterilization 15min, with sucrose, Chinese herbal medicine extract, honey, stabilizing agent and step 1) the blueberry pulp Homogeneous phase mixing that obtains, at 60 DEG C after abundant stirring and dissolving, homogeneous under 20MPa, then 33 DEG C are cooled in electric-field intensity 25kV/cm, burst length 300 μ s, pulse frequency 300Hz carries out high-pressure pulse electric sterilization 8min, adjusted to ph is 6.5, add 35% ferment at constant temperature 45min of leavening quality, then with the ramp to 42 DEG C of 0.9 DEG C/min, add 65% ferment at constant temperature 3h of leavening quality, last with the ramp to 52 DEG C of 0.7 DEG C/min, continuing fermentation is that 4.0 terminations are fermented and obtain blueberry Yoghourt to Yoghourt pH value,

3) preparation of blueberry sour milk powder: be that the waxy corn starch solution of 5% is respectively at 121 DEG C of sterilizing 9min by fresh skim milk and mass ratio, be cooled to 50 DEG C immediately, then with protective agent Homogeneous phase mixing, step 2 is added after abundant dissolving) in the blueberry Yoghourt that obtains, namely freeze drying obtains blueberry sour milk powder according to a conventional method;

4) compressing tablet: sprouted unpolished rice is crushed to particle diameter 0.2mm in 55 DEG C, with extractive of alfalfa, plant extracts, dextrin, the prebiotic factor, dolomol and step 3) the blueberry sour milk powder Homogeneous phase mixing that obtains, then 60 mesh sieves are crossed, making pressed candy through rushing rotary tablet machine more, obtaining the blueberry compressed tablet candy of delaying sanility.

Blueberry Yoghourt viable lactic acid bacteria content prepared by said method is 1.36 × 10 ¹²cFU/ml, the viable bacteria content of blueberry sour milk powder is 9.78 × 10 ¹¹cFU/g, the viable bacteria content of blueberry compressed tablet candy is 9.49 × 10 ¹¹cFU/g, under normal temperature, 36 months shelf-lifves.

Embodiment 3

Blueberry 85 parts, 50 parts, cow's milk, sucrose 45 parts; extractive of alfalfa 20 parts, sprouted unpolished rice 20 parts, Chinese herbal medicine extract 10 parts; clover nectar 10 parts, plant extracts 8 parts, leavening 7 parts; 5 parts, dextrin, the prebiotic factor 4 parts, protective agent 3 parts; fresh skim milk 1 part; 5% corn starch solution 0.5 part, dolomol 0.5 part, stabilizing agent 0.1 part.

Described leavening is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 25 parts, bifidobacterium bifidum 20 parts, bifidobacterium lactis 20 parts, streptococcus thermophilus 20 parts, bifidobacterium longum 16 parts, bifidobacterium infantis 16 parts, bifidobacterium adolescentis 15 parts, lactobacillus bulgaricus 13 parts, lactobacillus acidophilus 12 parts, Lactobacillus casei 8 parts, Lactobacillus rhamnosus 6 parts;

Described Lactobacillus plantarum pulvis is the pulvis 6:3 Homogeneous phase mixing in mass ratio prepared according to a conventional method by Lactobacillus plantarum CGMCCNo.4200 and Lactobacillus plantarum CGMCCNO.9488.

Described dextrin is maltodextrin, resistant dextrin 7:1 Homogeneous phase mixing in mass ratio.

Described prebiotic factor weight consists of: oligoisomaltose 10 parts, galactooligosaccharide 10 parts, FOS 8 parts, xylo-oligosaccharide 8 parts, stachyose 6 parts, soyabean oligosaccharides 6 parts, gossypose 6 parts, oligomeric maltose 5-7 part.

Described stabilizing agent is formed by the raw material Homogeneous phase mixing of following parts by weight: modified dietary fiber 16 parts, tremella polysaccharides 12 parts, 1 part, gelatin, pectin 1 part.

Preparation method, comprises the steps:

1) preparation of blueberry pulp: take blueberry by formula, blueberry is put into the supersonic wave cleaning machine filling 0.1% sodium bicarbonate solution, under room temperature, 200W, 35KHz clean 5min, drain, broken, in electric-field intensity 30kV/cm, burst length 600 μ s, pulse frequency 200Hz high-pressure pulse electric process 5min under room temperature, glue grinds, outlet pulp particle diameter is made to reach 150 μm, then under room temperature in electric-field intensity 20kV/cm, burst length 400 μ s, pulse frequency 200Hz high-pressure pulse electric process 3min, vacuum outgas obtains former pulp; Add cellulase, pectase, tannase and protease wherein, its content is made to be respectively 80ppm, 50ppm, 50ppm and 25ppm, in 45 DEG C of enzymolysis 1h, be warming up to 85 DEG C of insulation 3min, be cooled to room temperature immediately, 80 eye mesh screens filter, and namely filtrate reduced in volume obtains blueberry pulp to solid content to 30%;

2) preparation of blueberry Yoghourt: cow's milk after clean breast under room temperature in electric-field intensity 30kV/cm, burst length 400 μ s, pulse frequency 300Hz carries out high-pressure pulse electric sterilization 10min, with sucrose, Chinese herbal medicine extract, honey, stabilizing agent and step 1) the blueberry pulp Homogeneous phase mixing that obtains, at 55 DEG C after abundant stirring and dissolving, homogeneous under 18MPa, then 32 DEG C are cooled in electric-field intensity 20kV/cm, burst length 200 μ s, pulse frequency 200Hz carries out high-pressure pulse electric sterilization 5min, adjusted to ph is 6.0, add 30% ferment at constant temperature 0.5h of leavening quality, then with the ramp to 40 DEG C of 0.8 DEG C/min, add 70% ferment at constant temperature 2h of leavening quality, last with the ramp to 50 DEG C of 0.6 DEG C/min, continuing fermentation is that 4.5 terminations are fermented and obtain blueberry Yoghourt to Yoghourt pH value,

3) preparation of blueberry sour milk powder: be that the starch solution of 5% is respectively at 121 DEG C of sterilizing 8min by fresh skim milk and mass ratio, be cooled to 45 DEG C immediately, then with protective agent Homogeneous phase mixing, step 2 is added after abundant dissolving) in the blueberry Yoghourt that obtains, namely freeze drying obtains blueberry sour milk powder according to a conventional method;

4) compressing tablet: sprouted unpolished rice is crushed to particle diameter 0.1mm in 40 DEG C, with extractive of alfalfa, plant extracts, dextrin, the prebiotic factor, dolomol and step 3) the blueberry sour milk powder Homogeneous phase mixing that obtains, then 100 mesh sieves are crossed, making pressed candy through rushing rotary tablet machine more, obtaining the blueberry compressed tablet candy of delaying sanility.

Blueberry Yoghourt viable lactic acid bacteria content prepared by said method is 1.12 × 10 ¹²cFU/ml, the viable bacteria content of blueberry sour milk powder is 8.65 × 10 ¹¹cFU/g, the viable bacteria content of blueberry compressed tablet candy is 8.22 × 10 ¹¹cFU/g, under normal temperature, 30 months shelf-lifves.

Embodiment 4

Blueberry 95 parts, 60 parts, cow's milk, sucrose 65 parts, extractive of alfalfa 40 parts, sprouted unpolished rice 30 parts, Chinese herbal medicine extract 20 parts, sophorae honey 9 parts, honey of lychee flowers 9 parts, plant extracts 10 parts, leavening 9 parts, 9 parts, dextrin, the prebiotic factor 6 parts, protective agent 5 parts, fresh skim milk 3 parts, 5% potato starch solution 1 part, dolomol 1 part, stabilizing agent 0.3 part;

Described leavening is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 35 parts, bifidobacterium bifidum 25 parts, bifidobacterium lactis 23 parts, streptococcus thermophilus 23 parts, bifidobacterium longum 18 parts, bifidobacterium infantis 18 parts, bifidobacterium adolescentis 17 parts, lactobacillus bulgaricus 15 parts, lactobacillus acidophilus 14 parts, Lactobacillus casei 10 parts, Lactobacillus rhamnosus 8 parts;

Described Lactobacillus plantarum pulvis is the pulvis 8:5 Homogeneous phase mixing in mass ratio prepared according to a conventional method by Lactobacillus plantarum CGMCCNo.4200 and Lactobacillus plantarum CGMCCNO.9488.

Described dextrin is maltodextrin, resistant dextrin 8:2 Homogeneous phase mixing in mass ratio.

Described prebiotic factor weight consists of: oligoisomaltose 15 parts, galactooligosaccharide 15 parts, FOS 10 parts, xylo-oligosaccharide 10 parts, stachyose 8 parts, soyabean oligosaccharides 8 parts, gossypose 8 parts, oligomeric maltose 7 parts.

Described stabilizing agent is formed by the raw material Homogeneous phase mixing of following parts by weight: modified dietary fiber 20 parts, tremella polysaccharides 15 parts, 3 parts, gelatin, pectin 3 parts.

Preparation method, comprises the steps:

1) preparation of blueberry pulp: take blueberry by formula, blueberry is put into the supersonic wave cleaning machine filling 0.3% sodium bicarbonate solution, under room temperature, 400W, 45KHz clean 10min, drain, broken, in electric-field intensity 40kV/cm, burst length 800 μ s, pulse frequency 400Hz high-pressure pulse electric process 10min under room temperature, glue grinds, outlet pulp particle diameter is made to reach 250 μm, then under room temperature in electric-field intensity 30kV/cm, burst length 600 μ s, pulse frequency 400Hz high-pressure pulse electric process 5min, vacuum outgas obtains former pulp; Add cellulase, pectase, tannase and protease wherein, its content is made to be respectively 100ppm, 70ppm, 60ppm and 35ppm, in 55 DEG C of enzymolysis 3h, be warming up to 95 DEG C of insulation 5min, be cooled to room temperature immediately, 100 eye mesh screens filter, and namely filtrate reduced in volume obtains blueberry pulp to solid content to 40%;

2) preparation of blueberry Yoghourt: cow's milk after clean breast under room temperature in electric-field intensity 40kV/cm, burst length 600 μ s, pulse frequency 500Hz carries out high-pressure pulse electric sterilization 20min, with sucrose, Chinese herbal medicine extract, honey, stabilizing agent and step 1) the blueberry pulp Homogeneous phase mixing that obtains, at 65 DEG C after abundant stirring and dissolving, homogeneous under 22MPa, then 35 DEG C are cooled in electric-field intensity 30kV/cm, burst length 400 μ s, pulse frequency 400Hz carries out high-pressure pulse electric sterilization 10min, adjusted to ph is 7.0, add 40% ferment at constant temperature 0.5-1h of leavening quality, then with the ramp to 45 DEG C of 1.0 DEG C/min, add 60% ferment at constant temperature 4h of leavening quality, last with the ramp to 55 DEG C of 0.8 DEG C/min, continuing fermentation is that 3.5 terminations are fermented and obtain blueberry Yoghourt to Yoghourt pH value,

3) preparation of blueberry sour milk powder: be that the starch solution of 5% is respectively at 121 DEG C of sterilizing 10min by fresh skim milk and mass ratio, be cooled to 55 DEG C immediately, then with protective agent Homogeneous phase mixing, step 2 is added after abundant dissolving) in the blueberry Yoghourt that obtains, namely freeze drying obtains blueberry sour milk powder according to a conventional method;

4) compressing tablet: sprouted unpolished rice is crushed to particle diameter 0.3mm in 60 DEG C, with extractive of alfalfa, plant extracts, dextrin, the prebiotic factor, dolomol and step 3) the blueberry sour milk powder Homogeneous phase mixing that obtains, then 50 mesh sieves are crossed, making pressed candy through rushing rotary tablet machine more, obtaining the blueberry compressed tablet candy of delaying sanility.

Blueberry Yoghourt viable lactic acid bacteria content prepared by said method is 1.24 × 10 ¹²cFU/ml, the viable bacteria content of blueberry sour milk powder is 9.22 × 10 ¹¹cFU/g, the viable bacteria content of blueberry compressed tablet candy is 8.88 × 10 ¹¹cFU/g, under normal temperature, 32 months shelf-lifves.

The antibacterial effect test of embodiment 5 blueberry juice of the present invention

(1) get prepared by the embodiment of the present invention 2 concentrated before blueberry juice filtrate and the blueberries raw juice of commercially available same concentration, respectively after 0.22 μm of filtering with microporous membrane, for Determination of Antibacterial Activity.

(2) adopt nutrient agar to cultivate indicator bacteria, directly measure the antibacterial activity of blueberry juice with punch method.The cultured 100 μ L indicator bacteria bacterium suspension (1 × 10 of nutrient agar flat board coating ⁸cfu/ml), evenly punch with diameter 6mm card punch, every hole adds the blueberry juice 100 μ L prepared, and contrasts with sterilized water.Cultivate 16h for 37 DEG C, record inhibition zone size.Select the bacteriums such as Escherichia coli (Escherichiacoli), Staphylococcus aureus (Staphylococcusaureaus), Bacillus cercus (Bacilluscereus), pseudomonas aeruginosa (Pseudomonasaeruginosa), salmonella (Salmonellaenteritidis), bacillus subtilis (Bacillussubtilis) as strain subject.When doing mould bacteriostatic experiment, inoculation mould in the middle of PDA culture medium flat plate, after cultivating 1-2d, punching from bacterium colony 2cm place, every hole adds the blueberry juice 100 μ L prepared, and contrasts with sterilized water.Cultivate 3-4d, record antibacterial situation for 28 DEG C.The results are shown in Table 1:

Table 1: blueberry juice bacteriostatic activity result

Above result shows: blueberry juice of the present invention and commercially available blueberry juice all have certain inhibitory action to prokaryotic micro-organisms such as Escherichia coli (Escherichiacoli), staphylococcus aureus (Staphylococcusaureaus), Bacillus cercus (Bacilluscereus), salmonella (Salmonellaenteritidis) and bacillus subtilises (Pseudomonasaeruginosa).To filamentous fungi Trichoderma viride (Trichodermaviride), Mucor racemosus (Mucorracemosus), also there is certain inhibitory action, there is broad-spectrum antibacterial effect, but, blueberry juice effect prepared by the present invention is more remarkable, is about 2 times of commercially available blueberry juice fungistatic effect.Illustrate that the antibacterial active principle content such as blueberry juice aldehydes matter, organic acid, polysaccharide and anthocyan prepared by the present invention are high, refining losses is low, compared with prior art has certain advance and practicality.

It should be noted that: embodiment of the present invention 3-4 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect otherness.

Embodiment 6 protective agent effect test of the present invention

With the embodiment of the present invention 2 for test group; under other raw material and the identical condition of the condition such as component, technique; only protective agent kind is different; form blank group (not adding protective agent) and control group (sericin peptide taken is protective agent); viable bacteria content before measuring freeze-drying and after freeze-drying, result is as table 2:

Table 2: different group viable bacteria content

Project	Before freeze-drying	Blank group	Control group	Test group
					Viable bacteria content (CFU/g)	1.36×10 ¹²	2.72×10 ¹⁰	9.52×10 ¹⁰	9.78×10 ¹¹
Viable bacteria loss late (%)	0	98	93	28

Above result shows; use protective agent of the present invention as cryoprotector; the viable bacteria content of blueberry sour milk powder can be significantly improved; under similarity condition; after cool-drying, test group viable bacteria content is nearly 10 times of control group; be 36 times of blank group, viable bacteria loss late and control group, reduce by 65% and 70% respectively compared with blank group, there is very good anti-freezing effect.

Embodiment 7 pressed candy cosmetic result test of the present invention

The pressed candy embodiment of the present invention 2 prepared tests one month in Tianjin and Shanghai City, and everyone is each serving with 1 (200mg/ sheet), and every day three times, user 630 people, wherein 600 people's successfuls, remarkable rate is up to 95.2%.

Eater's effect analysis is illustrated:

1. man, Zhang, frequent On-line work, due to recently in the face of machine time is longer, color spot increases, and occurs that seasonality is casted off a skin, and through taking pressed candy after 2 weeks prepared by the embodiment of the present invention 2, color spot almost disappears completely.

2. female, willow, frequent skin lack of water, drying, although monthly do skin special and professional care 1 time, skin still relaxes, and lacks flexibility.Through taking the pressed candy of the embodiment of the present invention 2 preparation after 3 weeks, the colour of skin is lubricated, and fine and smooth, above-mentioned symptom thoroughly disappears.

3. female, Zhang, frequent On-line work, due to recently in the face of machine time is longer, color spot increases, and occurs that seasonality is casted off a skin, and through taking pressed candy after 2 weeks prepared by the embodiment of the present invention 2, color spot almost disappears completely.

4. female, Lee, complexion difference continues 1 year, and crow's feet, wrinkles on one's forehead etc. progressively manifest, and through taking the pressed candy of the embodiment of the present invention 2 preparation after 3 weeks, the colour of skin is normal, and crow's feet, wrinkles on one's forehead almost disappear completely.

5. female, Wei, owing to using color make-up or cosmetics, dry peeling, dim Huang are coarse on the face, and with age, skin problem gets more and more, and through taking pressed candy prepared by the embodiment of the present invention 2 after 3 weeks, the colour of skin is lubricated, and fine and smooth, above-mentioned symptom thoroughly disappears.

6. female, Huang, because frequent evening sleeps, stays up late or goes on business, face occurs that color spot, pore are thick, play acne, fat secretion is vigorous, and after taking the pressed candy week of the embodiment of the present invention 2 preparation, above-mentioned symptom disappears completely.

7. female, high certain, owing to using color make-up or cosmetics, dry peeling, dim Huang are coarse on the face, and with age, skin problem gets more and more, and through taking pressed candy prepared by the embodiment of the present invention 2 after 3 weeks, the colour of skin is lubricated, and fine and smooth, above-mentioned symptom thoroughly disappears.

8. man, Korea Spro, frequent skin lack of water, drying, although monthly do skin special and professional care 1 time, skin still relaxes, and lacks flexibility.Through taking the pressed candy of the embodiment of the present invention 2 preparation after 3 weeks, the colour of skin is lubricated, and fine and smooth, above-mentioned symptom thoroughly disappears.

9. female, Shen, because frequent evening sleeps, stays up late or goes on business, face occurs that color spot, pore are thick, play acne, fat secretion is vigorous, and through taking the pressed candy of the embodiment of the present invention 2 preparation after 3 weeks, above-mentioned symptom disappears completely.

10. female, goes into business certain, frequent skin lack of water, drying, and crow's feet, wrinkles on one's forehead etc. progressively manifest, and through taking the pressed candy of the embodiment of the present invention 2 preparation after 3 weeks, the colour of skin is normal, and crow's feet, wrinkles on one's forehead almost disappear completely.

Above result of the test shows, the pressed candy of delaying sanility of the present invention to the cutis laxa caused by different reason, nonelastic, color spot increases, wrinkles on one's forehead crow's feet increases etc., and symptom all has remarkable curative effect, there is good beautifying face and moistering lotion effect, and products taste of the present invention is good, be easy to take, free from extraneous odour, really realizes with nourishment maintenance face.

Viable lactic acid bacteria stable content test in the embodiment 8 pressed candy shelf-life of the present invention

Get pressed candy prepared by the embodiment of the present invention 2 in room temperature 22-25 DEG C, store 3 months, 6 months, 12 months, 24 months, 36 months respectively under ventilation condition and measure viable lactic acid bacteria content, result is as table 3:

Table 3: viable lactic acid bacteria content detection result in the shelf-life

Above result shows: the storage stability of pressed candy of the present invention is better, environment resistant (temperature, appropriateness, oxygen, illumination, moisture etc.) influence factor ability is large, in shelf-life, viable lactic acid bacteria content loss rate maximum (36 months) is 15%, higher than existing like product viable bacteria content, loss late is low, long shelf-life, send out other bioactive ingredients mirroring pressed candy simultaneously and there is same storage stability.

The sensory evaluation test of embodiment 9 pressed candy of the present invention

24 personnel are invited to judge with the pressed candy of commercially available two kinds of similar identical dates of manufacture pressed candy prepared by the embodiment of the present invention 2, sense organ is given a mark, wherein specialty and each 12 of layman, professional is young, middle aged, each 4 of old age, men and women half and half, layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half; Marking comprises outward appearance (20 points), quality (25 points), local flavor (30 points), mouthfeel (25 points) four aspects, and marking personnel independently carry out, and are independent of each other, accurate to ensure judging result.Add up judging result, equal score value gets approximation, retains integer, specifically in table 4:

Table 4: sensory evaluation statistics

Note: show significant difference (P < 0.05) with marking different lowercase alphabet in a line, mark different capitalization and represent that difference extremely significantly (P < 0.01), indicate same letter and represent difference not significantly (P > 0.05).

Above result shows, any one all will obviously be better than commercially available pressed candy to pressed candy prepared by the present invention from outward appearance, quality, local flavor and mouthfeel, particularly outward appearance, local flavor and mouthfeel are fabulous, and also the consumer of applicable Different age group, different hierarchy of consumption eats simultaneously.

It should be noted that: pressed candy prepared by embodiment of the present invention 3-4 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect otherness.

The probiotic test of embodiment 10 pressed candy of the present invention

By pressed candy prepared by the embodiment of the present invention 2, being prepared into viable count with sterilized water is 2 × 10 ¹⁰the probiotic solution of CFU/mL, saves backup in 4 DEG C;

1, the 10mL probiotic solution kept of going bail for is injected in test tube 1, adopts ten times of stepwise dilutions to 10 ^-8, get 1mL dilution on flat board, the MRS agar medium being cooled to 45 DEG C poured in dull and stereotyped (sterilizing), shake up rapidly after sterilizing.Again the test tube 2 that 10mL probiotic solution is housed is placed in 80-90 DEG C of water-bath and heats 15-25min, get the probiotic solution after heating and carry out ten times of stepwise dilutions to 10 ^-8, get 1mL dilution on flat board, the MRS agar medium being cooled to 45 DEG C poured in dull and stereotyped (sterilizing) upper and shake up rapidly after sterilizing.Finally the flat board before heating and after heating is all cultivated 24h under 35 DEG C of conditions, calculate the quantity before and after heating.

Result shows, Viable detection reaches more than 88%.

2, the resistance test of SGF and intestinal juice: the hydrochloric acid 16.4mL adding distil water dilution of getting 100g/L, pH value is made to be respectively 1.5,2.5 and 3.5, get 100mL dilute hydrochloric acid solution, add 1g pepsin respectively, it is made fully to dissolve, obtain SGF, miillpore filter degerming (0.22 μm) is for subsequent use.Get potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, by 0.1moL/L sodium hydroxide solution adjust ph to 6.8; Separately get trypsase 10g, the 100mL that adds water makes dissolving, and after two liquid mixing, be diluted with water to 1000ml, obtain simulated intestinal fluid, miillpore filter degerming (0.22 μm) is for subsequent use.Get the probiotic solution that 1mL keeps and join (i.e. ten times of stepwise dilutions) in the SGF of 9mL, and fully mix on the oscillator rapidly, be then placed in 30-45 DEG C of quiescent culture 2-4h.Take out nutrient solution when 1h, 2h, 3h, 4h respectively and count remaining viable count immediately, compare with former viable count, result shows, Viable detection is 98%.Then each 1mL of the nutrient solution digesting different time is taken in simulated gastric fluid, being inoculated in 9mLpH value is respectively in the simulated intestinal fluid of 6.8, be placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h sampling, measure its viable count, compare with former viable count, result shows that Viable detection is 99%.

3, simulating the resistance test of cholate: the solution making 1g/L with pancreatin, and the pig cholate adding 0.8% in the solution, is 8.0 with the NaOH adjustment pH of 10%, then uses 0.45 μm of micro-filtrate membrane filtration and degerming.The probiotic solution kept by 0.5mL is inoculated into 4.5mL and simulates in cholate, obtains nutrient solution, count remaining viable count after cultivating 24h.By nutrient solution ten times of stepwise dilutions to 10 in sterile saline ^-8, and pour into MRS, be then placed in 35 DEG C of quiescent culture 24h.Result shows that Viable detection is 99%.

Above result of the test shows, the probio in pressed candy of the present invention probiotic (heat resistance, resistance to pH, bile tolerance) is comparatively strong, is very applicable to human intestines and stomach's environment, and in simulated gastrointestinal environments, survival rate is large, effectively can improve gastrointestinal function.

Embodiment 11 pressed candy mouse intestinal of the present invention performance test

Choose common Kunming small white mouse 60, male and female half and half, 18-20g, conventional word is supported.Therefrom random choose 40,9:00 gavage Lincomycin Hydrochloride 0.2mL every morning (20mg)/only, other as a control group, every day same time gavage equivalent sterile saline, continuous one week, prepares the mouse model of intestinal bacilli illness.Model group mouse diet declines, and do not occur dead and phenomenon of significantly suffering from diarrhoea, arrange soft excrement, profile normal aqueous divides more, and bedding and padding are moist.By 40 intestinal bacilli illness mouse, be divided into 2 groups at random, one group 20 only as treatment group, the probiotic solution 0.5ml (2 × 10 that every day, gavage was kept ¹⁰cfu/ml)/only, another 20 as natural recovering group, every day same time gavage equivalent sterile saline, continuous two weeks.21 days whole experimental periods, observe growth and the defecation situation of small white mouse every day, weigh in the 8th, 21 day mouse to pressed candy treatment group and natural recovering group, calculate each group of weight average growth rate, result is as table 5; Within every 5 days, survey each group of stool in mice Escherichia coli quantity, calculate average, result is as table 6.Get stool in mice and be about 0.1g, in aseptic operating platform, add 3 beades (adding 0.5mL dilution with 0.1g excrement sample), dilute and inoculate maconkey agar culture medium, calculate every gram of coliform count wet just.

Table 5: mouse Gain weight

Grouping	Average starting weight (g/ only)	Average end heavy (g/ only)	Average growth rate (%)
				Natural recovering group	20.69±1.33	27.34±1.59	32.14 ^a
Treatment group	20.41±1.45	34.28±1.62	67.96 ^b

Table 6: the situation of coliform count in stool in mice

Pressed candy treatment group Mouse Weight average growth rate (67.96%) is significantly higher than natural recovering group (32.14%); Solution rear intestinal Escherichia coli quantity of feeding significantly declines, reduce by 97.07%, significantly lower than natural recovering group (24.78%), show the probio rapid field planting in small white mouse enteron aisle in pressed candy of the present invention, form dominant microflora, and effectively suppress the growth and breeding of the pathogens such as Escherichia coli, and resident time is long, continues, effectively improve enteron aisle performance.

Embodiment 12 pressed candy of the present invention is on the impact of immunity of organisms

1 experiment purpose

By exercise tolerance test (mouse forced swimming), verify raising immunity, the antifatigue effect of pressed candy of the present invention.

2 experiment materials and reagent

2.1 for reagent thing:

Commercially available beautifying face and moistering lotion pressed candy (G1); Commercially available beautifying face and moistering lotion pressed candy (G2); Pressed candy (G3-G5) prepared by embodiment of the present invention 2-4.

2.2 reagent:

Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing; The concentrated sulfuric acid (AR), Nanjing Chemistry Reagent Co., Ltd.; Physiological saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..

3. animal used as test

ICR mouse, ♂, cleaning grade, body weight 18-22g, is provided by Beijing Medical University's comparative medicine center, the free diet of experimental session mouse.

4. key instrument

Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof company; Water-bath: DK-S26 type, the grand experimental facilities Co., Ltd of upper Nereid; Electronic scale: BS224S type, Sartorius company; Stopwatch, thermometer

5. experiment grouping

5.1 dosage groupings and given the test agent give the time: at random mouse is divided into 8 groups, often organize 10,1st group to the 5th group respectively to the medicine of G1 ~ G5,6th group is blank group, give isopyknic distilled water, the often every average daily gavage of group 1 time, gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.

5.2 sample preparations: the 1st group to the 5th group: take 2.25g drug sample, be assigned to 150ml with distilled water; Blank group (the 6th group): distilled water 150ml.

6. experimental technique

After 6.1 swimming with a load attached to the body experiment last administration 30min, put mouse in swimming trunk, the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of mouse root of the tail portion load 5% body weight, and the swimming of record mouse starts to the dead time, as mouse swimming time.

After 6.2 mice serum urea measure last administration 30min, be not swimming with a load attached to the body 90min in the water of 30 DEG C in temperature, eyeball blood sampling 0.5mL (not adding anti-coagulants) is plucked after rest 60min, put 4 DEG C of refrigerator 3h, the centrifugal 15min of 2000r/min after blood clotting, gets serum and send clinical laboratory of affiliated hospital of Beijing Medical University to detect.

After the mensuration last administration 30min of 6.3 hepatic glycogen, be not swimming with a load attached to the body 90min in the water of 25 ± 1 DEG C in temperature, cervical dislocation puts to death mouse, clean with physiological saline, and with after filter paper suck dry moisture, accurately taking liver 100mg, hepatic glycogen detection kit detects Mouse Liver glycogen content.

Take a blood sample after the mensuration last administration 30min of 6.4 blood lactase acid, then do not bear a heavy burden stops after temperature is the water went swimming 10min of 30 DEG C.Lactic acid instrument assay method: after rest 20min, each blood sampling 20 μ L add in 40 μ L rupture of membranes liquid before swimming, after swimming, after swimming respectively, the smudge cells lactic acid instrument that fully vibrates immediately measures.(blood lactase acid TG-AUC=5 × (the blood lactase acid value of the rear 20min of blood lactase acid value+2 × swimming of the rear 0min of front blood lactase acid value+3 × swimming that swims)

7. observation index walking weight load, blood lactase acid, urea, glycogen initial value

8. statistical method experimental data is used represent, employing t inspection is compared between organizing

9. experimental result

9.1 pressed candies of the present invention are on the impact of Mouse Weight

Each group of mouse is after giving G1 ~ G5 medicine, before, in, shown in post-weight sees the following form respectively, the original body mass of each group mouse and increase weight body weight and the more equal no difference of science of statistics of control group (P > 0.05), show G1 ~ G5 medicine all without obvious toxicity.Experimental result refers to table 7.

The original body mass of table 7 swimming with a load attached to the body experiment mice, mid-term body weight and terminate body weight

9.2 pressed candies of the present invention are on the impact of mice burden swimming time

After per os gives mouse G1 ~ G5 medicine, G1 ~ G2 medicine compares with blank group, can obviously extend the mice burden swimming time, there is significant difference (P < 0.05), pressed candy G3 ~ G5 medicine of the present invention compares with blank group, can the significant prolongation mice burden swimming time, there is pole significant difference (P < 0.01), and be obviously better than G1 ~ G2 medicine.The results detailed in Table 8.

Table 8 pressed candy is on the impact of mice burden swimming time

" * " p<0.05vs blank;

" * * " p<0.01vs blank;

9.3 pressed candies of the present invention are on the impact of blood lactase acid before and after mouse movement

After per os gives mouse pressed candy of the present invention, pressed candy G3 ~ G5 medicine of the present invention to compare with control group blood lactase acid TG-AUC after mouse movement significant difference (P < 0.05), decrease though G1 ~ G2 medicine group Mouse Blood lactic acid TG-AUC compares with control group, and no difference of science of statistics (P > 0.05).The results are shown in Table 9.

Table 9 pressed candy of the present invention is on the impact of blood lactase acid level before and after mouse movement

" * " p<0.05vs blank;

9.4 pressed candies of the present invention are on the impact of Mouse Liver glycogen

After per os gives mouse G1 ~ G5 medicine, G1 ~ G2 medicine compares with blank group, Mouse Liver glycogen content all has obvious rising, there is significant difference (P < 0.05), pressed candy G3 ~ G5 medicine of the present invention compares with blank group, Mouse Liver glycogen content all has obvious rising, has pole significant difference (P < 0.01), and is obviously better than G1 ~ G2 medicine.The results detailed in Table 10.

Table 10 pressed candy of the present invention is on the impact of Mouse Liver glycogen content

" * " p<0.05vs blank;

" * * " p<0.01vs blank;

9.5 pressed candies of the present invention are on the impact of mice serum urea

After per os gives mouse G1 ~ G5 medicine, G1 ~ G2 medicine group compares with blank group, after mouse movement, serum urea content all has obvious reduction, there is significant difference (P < 0.05), pressed candy G3 ~ G5 medicine of the present invention compares with blank group, after mouse movement, serum urea content all has obvious reduction, has pole significant difference (P < 0.01), and is obviously better than G1 ~ G2 medicine.The results detailed in Table 11.

Table 11 pressed candy of the present invention is on the impact of mice serum urea content

" * " p<0.05vs blank;

" * * " p<0.01vs blank;

10. experiment conclusion

This experiment is mainly through mice burden swimming experiment, and the deposit simultaneously detecting Mouse Liver glycogen observes the effect that pressed candy of the present invention improves immunity, antifatigue.Preliminary Results shows below:

1, G3 ~ G5 pressed candy of the present invention all can extend the mice burden swimming time (P < 0.01), and successful is better than the pressed candy of other G1 ~ G2.

2, biochemistry detection aspect display, the lactic acid content that after each dosage group of G3 ~ G5 pressed candy of the present invention all can reduce motion, in mice serum, glucose anerobic glycolysis produces, compare with control group and have significant difference (P < 0.05), and although the pressed candy of other G1 ~ G2 also can reduce the lactic acid content that in the rear mice serum of motion, glucose anerobic glycolysis produces, but compare with control group, no difference of science of statistics (P > 0.05);

3, each dosage group of G3 ~ G5 pressed candy of the present invention all can significantly improve the deposit (P < 0.01) of glycogen in mouse liver, and successful is better than the pressed candy of other G1 ~ G2;

4, high lithemia model finds, G3 ~ G5 pressed candy of the present invention significantly can reduce the content (P < 0.01) of the rear urea in serum of mouse swimming, and successful is better than other G1 ~ G2 pressed candy;

11. conclusions

Above-mentioned experiment proves that pressed candy of the present invention can significantly improve immunity of organisms, improve muscle power and the endurance of mouse, the content of urea in serum and lactic acid after reduction mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, contribute to the fatigue that alleviation exercise load causes; The time that mice burden swimming to power exhausts can be extended.

Claims

1. a blueberry compressed tablet candy for delaying sanility, the raw material primarily of following components by weight percent obtains: blueberry 85-95 part, cow's milk 50-60 part, sucrose 45-65 part, extractive of alfalfa 20-40 part, sprouted unpolished rice 20-30 part, Chinese herbal medicine extract 10-20 part, honey 10-18 part, plant extracts 8-10 part, leavening 7-9 part, dextrin 5-9 part, prebiotic factor 4-6 part, protective agent 3-5 part, fresh skim milk 1-3 part, 5% starch solution 0.5-1 part, dolomol 0.5-1 part, stabilizing agent 0.1-0.3 part;

The preparation method of described plant extracts, comprises the steps: to count by weight, takes pearl 20-40 part, root of Dahurain angelica 20-30 part, lavender 20-30 part, Radix Angelicae Sinensis 18-20 part, red sage root 12-15 part, rose 8-12 part, sophora flower 6-10 part, peach blossom 5-8 part, Chinese trumpet creeper 5-8 part, safflower 4-6 part; Put in the supersonic wave cleaning machine filling 0.1-0.3% sodium bicarbonate solution and clean 3-5min in 200W, 30KHz, drain, it is below 2mm that above-mentioned plant material is crushed to particle diameter, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtaining mixed material, control temperature 40-50 DEG C, be 3-4 by lactic acid adjust ph, under power 150-300W condition, carry out Microwave Extraction 20-40min, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Then the mixed enzyme adding mixed material gross weight 0.5-1.5% carries out enzymolysis, be 5.5-6.8 by lactic acid adjust ph, enzymolysis 2-4h, finally add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1-3, control temperature keeps 3-4h to 60-70 DEG C, filters, obtains the first filtrate; Add the water of filter residue 1-3 times of weight, control temperature 85-95 DEG C keeps 1-2h, is then cooled to 25-35 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 3-5:1-3, Homogeneous phase mixing, being evaporated to solid content is more than 15%, and freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains plant extracts;

2. the blueberry compressed tablet candy of a kind of delaying sanility as claimed in claim 1, it is characterized in that, the preparation method of described extractive of alfalfa, comprise the following steps: red clover, alfalfa, northeast soybean, onion, ginkgo, aloe are put into respectively the supersonic wave cleaning machine that 0.3-0.5% sodium bicarbonate solution is housed and clean 12-15min in 100-300W, 20-40KHz, drain, 10-15:5-7:3-5:1-3:1-3:1-2 Homogeneous phase mixing in mass ratio, then in-18--23 DEG C of freezing 5-10min; Be crushed to particle diameter 0.5-1.5mm immediately; To put in container and to add the benzinum of 10-15 times of weight, putting into water bath chader 35-45 DEG C of concussion 1-3h, suction filtration, drying to obtain mixed powder; Add mixed powder quality 15-25 60% ethanol doubly, be 3.5-5.5 by lactic acid adjust ph, at electric-field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, carries out high-pressure pulse electric process under pulse frequency 200-400Hz condition; Then in 30-40 DEG C of insulation 1-2h, be then warming up to 40-50 DEG C under power 150-300W condition, carry out microwave irradiation extraction 15-20min, under power 200-400W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Extracting liquid filtering, obtains filtrate, and filter residue 1-3 times of weight 45-60 DEG C of water rinse 3 times, rinsing liquid and filtrate merge, Homogeneous phase mixing, and being evaporated to solid content is more than 15%, and freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains extractive of alfalfa.

3. the blueberry compressed tablet candy of a kind of delaying sanility as claimed in claim 1, it is characterized in that, described protectant preparation method, comprise the steps: winter rye, Ammopiptanthus mongolicus, Rhodiola algida Var. tangutica leaf, sharkskin collagen cleans respectively, drain, 8-10:4-6:3-5:2-4 Homogeneous phase mixing in mass ratio, adding mixed material quality 0.1-1 pH value is doubly that the lactic acid of 3.8-4.5 soaks 3-8h, pulverize immediately after-18--22 DEG C of freezing 1-2h, freezing thickness of feed layer 3-5cm, crushed material particle diameter 0.5-3mm, then crushed material quality 10-20 water is doubly added, be 3.5-5.5 by lactic acid adjust ph, at electric-field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, high-pressure pulse electric process 20-30min is carried out under pulse frequency 200-300Hz condition, then under power 150-300W condition, carry out microwave irradiation in room temperature and extract 15-20min, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously, add the compound enzyme of extract quality 3-5%, in 45-55 DEG C of enzymolysis 30-50min, enzymolysis liquid filters, filtrate is concentrated, low-temperature grinding to particle diameter is that namely 0.1-0.3mm obtains protective agent,

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 3-5:3-5:2-4:2-4:1-3 Homogeneous phase mixing in mass ratio.

4. the blueberry compressed tablet candy of a kind of delaying sanility as claimed in claim 1, it is characterized in that, the preparation method of described sprouted unpolished rice, comprise the steps: brown rice to be placed on room temperature, power 100-300W, frequency 20-30KHz ultrasonic cleaning 5-10min in the supersonic wave cleaning machine that 0.1-0.3% sodium bicarbonate solution is housed, rinsing, to drain, put in 16-18 DEG C of water and soak 2-4h, simultaneously in electric-field intensity 20-40kV/cm, burst length 300-500 μ s, pulse frequency 100-300Hz carries out high-pressure pulse electric process, until brown rice moisture content is 35-38%; Brown rice after immersion drains, dark germination is carried out in growing floor, dark germination temperature keeps 23-25 DEG C, the dark germination time is 15-17h, and the aqueous solution of period sprinkling containing sodium ion 35-45mg/L, potassium ion 25-35mg/L, magnesium ion 12-18mg/L, zinc ion 12-15mg/L and calcium ion 8-10mg/L is until brown rice moisture content is to 43-45%; Stop spraying, in growing floor, proceed dark germination, dark germination temperature keeps 24-26 DEG C, and the dark germination time is 13-15h; Brown rice after germinateing again is dry in frequency 2450MHz, power 3000W, temperature 50-60 DEG C, thickness of feed layer 2-4cm condition microwave irradiation, and wherein microwave irradiation 10s, interval 10s, being dried to moisture is 12-14%, packs and obtains sprouted unpolished rice.

5. the blueberry compressed tablet candy of a kind of delaying sanility as claimed in claim 1, it is characterized in that, the preparation method of described Chinese herbal medicine extract, comprise the steps: to count by weight, take Radix Astragali 15-17 part, motherwort 15-17 part, barrenwort 13-16 part, tuber of multiflower knotweed 12-15 part, dried orange peel 12-15 part, rhizoma zingiberis 8-12 part, Radix Codonopsis 6-10 part, seed of Chinese dodder 5-8 part; Put in the supersonic wave cleaning machine filling 0.1-0.3% sodium bicarbonate solution and clean 10-15min in 200W, 30KHz, drain, said herbal medicine being crushed to particle diameter is below 2mm, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixed material, control temperature 70-90 DEG C keeps 2-4h, then 40-50 DEG C is cooled to, be 3.5-4.5 by lactic acid adjust ph, Microwave Extraction 10-15min is carried out under power 150-300W condition, under power 200-300W, frequency 30-40KHz condition, carry out ultrasonic assistant extraction simultaneously; Then the complex enzyme adding mixed material gross weight 0.5-1.5% carries out enzymolysis, be 5.5-6.8 by lactic acid adjust ph, enzymolysis 2-4h, finally add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1-3, control temperature keeps 3-4h to 60-78 DEG C, filters, obtains the first filtrate; Add the water of filter residue 1-3 times of weight, control temperature 85-95 DEG C keeps 1-3h, is then cooled to 25-35 DEG C, filters, obtains the second filtrate; First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, Homogeneous phase mixing, being evaporated to solid content is more than 15%, and freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains Chinese herbal medicine extract;

6. the blueberry compressed tablet candy of a kind of delaying sanility as claimed in claim 1, is characterized in that, described stabilizing agent is formed by the raw material Homogeneous phase mixing of following parts by weight: modified dietary fiber 16-20 part, tremella polysaccharides 12-15 part, gelatin 1-3 part, pectin 1-3 part;

The preparation method of described modified dietary fiber, comprise the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber 4-6:3-5:2-4 Homogeneous phase mixing in mass ratio, add its quality 3-7 water doubly, room temperature 200-300W, 35-40KHz condition ultrasonic extraction 10-15min, then at electric-field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric process 10-15min under pulse frequency 200-300Hz condition; Be 4.5-6.5 by lactic acid adjust ph, add the biology enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min; Enzymolysis liquid reduced pressure concentration, freeze drying, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains modified dietary fiber;

7. the blueberry compressed tablet candy of a kind of delaying sanility as claimed in claim 1, it is characterized in that, described leavening is formed by the pulvis Homogeneous phase mixing of following parts by weight: Lactobacillus plantarum 25-35 part, bifidobacterium bifidum 20-25 part, bifidobacterium lactis 20-23 part, streptococcus thermophilus 20-23 part, bifidobacterium longum 16-18 part, bifidobacterium infantis 16-18 part, bifidobacterium adolescentis 15-17 part, lactobacillus bulgaricus 13-15 part, lactobacillus acidophilus 12-14 part, Lactobacillus casei 8-10 part, Lactobacillus rhamnosus 6-8 part.

8. the blueberry compressed tablet candy of a kind of delaying sanility as claimed in claim 7, it is characterized in that, Lactobacillus plantarum pulvis is the pulvis 6-8:3-5 Homogeneous phase mixing in mass ratio prepared according to a conventional method by Lactobacillus plantarum CGMCCNo.4200 and Lactobacillus plantarum CGMCCNO.9488.

9. the preparation method of a kind of blueberry compressed tablet candy of delaying sanility as claimed in claim 1, is characterized in that, comprise the steps:

10. preparation method as claimed in claim 9, is characterized in that, step 3) starch is waxy corn starch in described starch solution.