CN110195090A - The preparation method and gained sea cucumber intestine ovum polypeptide powder of sea cucumber intestine ovum polypeptide powder - Google Patents

The preparation method and gained sea cucumber intestine ovum polypeptide powder of sea cucumber intestine ovum polypeptide powder Download PDF

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Publication number
CN110195090A
CN110195090A CN201910407097.0A CN201910407097A CN110195090A CN 110195090 A CN110195090 A CN 110195090A CN 201910407097 A CN201910407097 A CN 201910407097A CN 110195090 A CN110195090 A CN 110195090A
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sea cucumber
cucumber intestine
peptide fragment
intestine ovum
preparation
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柯亚夫
仇登宇
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Chizhou Moon Bay Biotechnology Co Ltd
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Chizhou Moon Bay Biotechnology Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/385Concentrates of non-alcoholic beverages
    • A23L2/39Dry compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/18Peptides; Protein hydrolysates
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/34Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The present invention proposes the preparation method and gained sea cucumber intestine ovum polypeptide powder of a kind of sea cucumber intestine ovum polypeptide powder, belong to polypeptide and enzyme processing technique field, nutriment can be discharged into solution by the preparation method by the method for biological enzymolysis, obtain the peptide solution of degree of hydrolysis height, active peptide rich content, rational technology, strong operability, are easy to realize industrial production.The technical solution includes obtaining the sea cucumber intestine ovum polypeptide powder of different peptide fragments by fermentation enzymatic hydrolysis, high-temperature inactivation, classification membrane filtration pattern.During the deep development that the present invention can be applied to sea cucumber intestine ovum utilizes.

Description

The preparation method and gained sea cucumber intestine ovum polypeptide powder of sea cucumber intestine ovum polypeptide powder
Technical field
The invention belongs to the preparation method of polypeptide and enzyme processing technique field more particularly to a kind of sea cucumber intestine ovum polypeptide powder and Gained sea cucumber intestine ovum polypeptide powder.
Background technique
Sea cucumber intestine ovum high-quality protein rich in and 8 kinds of amino acid needed by human and various trace elements, have compared with Strong nourishing and strengthening Yin and Yang, improvement digestive system microcirculation, enhancing myocardial nutrition blood circulation flow, inhibition growth of tumour cell and expansion Scattered effect.
However, it is improper due to traditional processing mode, so that sea cucumber intestine ovum is removed frequently as leftover bits and pieces, cause sea Join the waste of resource.Sea cucumber intestine ovum polypeptide refers to the small-molecular peptides obtained after protease hydrolytic and separating-purifying, has a variety of function Effect, such as lowering blood pressure and blood fat, antiatherosclerosis, antifatigue, antibacterial, improve immunity, anti-aging and anti-oxidant all More physiological functions have other nutriment irreplaceable roles to human physiological functions.Since sea cucumber intestine ovum polypeptide has The special physicochemical properties such as good dissolubility, stability, low viscosity, absorption easy to digest, no antigen, edible safety, Therefore, sea cucumber intestine ovum polypeptide has higher biological value than other common cucumber products.
Modern biotechnology metabolism research discovery: the protein of human consumption is after gastral a variety of enzyme hydrolysis, more It is directly to be absorbed in the form of low peptide, there is higher nutritive value and biological value.Enzyme hydrolysis method has obtained biologically active peptide Through the main means for becoming industrialized production biologically active peptide.However, at present using enzymolysis process to sea cucumber intestine ovum at When reason, not already available degree of hydrolysis is high, therefore how active peptide rich content biologically active peptide improves existing enzymatic hydrolysis work Skill is particularly important the exploitation of sea cucumber intestine ovum polypeptide.
Summary of the invention
The present invention proposes the preparation method and gained sea cucumber intestine ovum polypeptide powder of a kind of sea cucumber intestine ovum polypeptide powder, the preparation method Nutriment can be discharged into solution by the method for biological enzymolysis, it is molten to obtain degree of hydrolysis height, the peptide of active peptide rich content Liquid, rational technology, strong operability, is easy to realize industrial production.
In order to achieve the above object, the present invention provides a kind of preparation methods of sea cucumber intestine ovum polypeptide powder, including following step It is rapid:
After the degreasing sea cucumber intestine egg powder that pre-sifted is chosen is broken, enzyme preparation is added, persistently stirring is digested in fermentor;
Enzymatic hydrolysis makes enzyme complete deactivation using high-temperature inactivation, is then centrifuged, is centrifuged to enzymolysis liquid in terminating Liquid;
The peptide fragment III of the peptide fragment I of molecular cut off > 5000Da, the peptide fragment II of 2500-5000Da, 1000-2500Da respectively With the peptide fragment IV of < 1000Da;
Each peptide fragment of retention is freeze-dried, the sea cucumber intestine ovum polypeptide powder of different peptide fragments is obtained.
Preferably, the enzyme preparation being added is made of neutral proteinase, alkali protease and de- bitter food flavor enzyme.
In above-mentioned technical proposal, compatibility is carried out using above-mentioned enzyme, reason is that sea cucumber intestine ovum liquid pH7.2-8.3 omits alkali Property, neutral proteinase and alkali protease combination are selected, enzymolysis efficiency and enzymatic activity can be effectively improved;And add de- bitter food flavor enzyme It is then and to easily cause taste variation since low molecular weight peptide fragment content accounting is larger in product, and de- bitter food flavor enzyme can then have The bitter taste of effect control peptide, to obtain that properties of product are higher, the better product of taste.
Preferably, the additive amount for the neutral proteinase being added is the 1-4% of degreasing sea cucumber intestine ovum, the basic protein of addition The additive amount of enzyme is the 2-6% of degreasing sea cucumber intestine ovum, and the additive amount of the de- bitter food flavor enzyme of addition is the 0.5- of degreasing sea cucumber intestine ovum 1.5%.
Preferably, the temperature of fermentor is 50-60 DEG C, enzymolysis time 2-7h.
Preferably, the temperature of high-temperature inactivation is 90-95 DEG C, duration 15-25min.
The present invention provides a kind of, and the preparation method of sea cucumber intestine ovum polypeptide powder as described in any of the above-described technical solution obtains Different peptide fragments sea cucumber intestine ovum polypeptide powder.
Preferably, including the peptide fragment I of molecular weight > 5000Da, the peptide fragment II of 2500-5000Da, 1000-2500Da The sea cucumber intestine ovum polypeptide powder of IV 4 peptide fragments of peptide fragment of peptide fragment III and molecular weight < 1000Da.
Preferably, the peptide fragment IV of < 1000Da accounts for 92% of total weight or more.
The present invention also provides a kind of health care product or cosmetics, < 1000Da peptide fragment described in techniques described above scheme Sea cucumber intestine ovum polypeptide powder is main component.
The present invention also provides a kind of liquid or solid beverage, < 1000Da peptide fragment described in techniques described above scheme Sea cucumber intestine ovum polypeptide powder is primary raw material.
Compared with prior art, the advantages and positive effects of the present invention are:
The preparation method of sea cucumber intestine ovum polypeptide powder provided by the invention can be released nutriment by the method for biological enzymolysis It is put into solution, obtains the peptide solution of degree of hydrolysis height, active peptide rich content, wherein can direct absorbed < 1000Da peptide fragment Content is up to 92% or more.The preparation method rational technology, strong operability, are easy to realize industrial production.In addition, institute of the present invention In the method final step of offer when obtaining the peptide solution of each peptide fragment by membrane filtration technique, sea cucumber intestine can be also effectively overcome The high problem of ash content in ovum polypeptide.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects It encloses.
Embodiment 1
Degreasing sea cucumber intestine ovum raw material is subjected to prescreening, it is desirable that raw material free from extraneous odour, impurity etc. crush it, it is desirable that Then degreasing sea cucumber intestine ovum is put into fermentor, and adds corresponding enzyme according to the content of degreasing sea cucumber intestine ovum by partial size >=60 mesh Preparation, persistently stirring carries out enzymatic hydrolysis 7h in 50 DEG C of fermentor;Wherein, the enzyme preparation being added includes neutral proteinase, alkali Property protease, de- bitter flavor enzyme preparation, the additive amount for the neutral proteinase being added is the 1% of degreasing sea cucumber intestine ovum, addition The additive amount of alkali protease is the 2% of degreasing sea cucumber intestine ovum, and the additive amount of the de- bitter food flavor enzyme of addition is degreasing sea cucumber intestine ovum 0.5%;
Enzymatic hydrolysis, in 90 DEG C of high-temperature inactivation 25min, makes enzyme complete deactivation, then enzymolysis liquid is centrifuged in terminating, Obtain centrifugate;
By ceramic membrane equipment to centrifugate carry out classified filtering, respectively molecular cut off > 5000Da peptide fragment I, The peptide fragment IV of the peptide fragment II of 2500-5000Da, the peptide fragment III of 1000-2500Da and < 1000Da;
Each peptide fragment of retention is freeze-dried in -40 DEG C, obtains the sea cucumber intestine ovum polypeptide powder of different peptide fragments.
Embodiment 2
Identical as 1 step of embodiment, difference place is: the temperature of fermentor is 55 DEG C, enzymolysis time 4.5h;High temperature The temperature of inactivation is 92 DEG C, time 20min;The additive amount for the neutral proteinase being added is the 2% of degreasing sea cucumber intestine ovum, is added The additive amount of the alkali protease entered is the 4% of degreasing sea cucumber intestine ovum, and the additive amount of the de- bitter food flavor enzyme of addition is degreasing sea cucumber The 1.0% of intestines ovum.
Embodiment 3
Identical as 1 step of embodiment, difference place is: the temperature of fermentor is 60 DEG C, enzymolysis time 2h;High temperature goes out Temperature living is 90 DEG C, time 15min;The additive amount for the neutral proteinase being added is the 4% of degreasing sea cucumber intestine ovum, is added The additive amount of alkali protease be the 6% of degreasing sea cucumber intestine ovum, the additive amount of the de- bitter food flavor enzyme of addition is degreasing sea cucumber intestine The 1.5% of ovum.
The sea cucumber intestine ovum polypeptide molecular weight testing result of different peptide fragments prepared by embodiment 1-3 is as shown in table 1.
1 sea cucumber intestine ovum polypeptide molecular weight testing result of table
From the data in table 1, it can be seen that preparation method provided in an embodiment of the present invention can digest to obtain degree of hydrolysis height, active peptide content The peptide solution of multiple peptide fragments abundant, wherein divided by molecular weight, the peptide fragment content of 1000 molecular weight of < up to 92% or more, And there is absolute predominance in the peptide fragment being distributed at four.The peptide fragment of 1000 molecular weight of < digested in above-described embodiment 1-3 After tested, up to 98%, absorptivity can directly be absorbed by the body dissolubility up to 99.6% or more, have higher Nutritive value and biological value.

Claims (10)

1. the preparation method of sea cucumber intestine ovum polypeptide powder, which comprises the following steps:
After the degreasing sea cucumber intestine egg powder that pre-sifted is chosen is broken, enzyme preparation is added, persistently stirring is digested in fermentor;
Enzymatic hydrolysis makes enzyme complete deactivation using high-temperature inactivation, is then centrifuged to enzymolysis liquid, obtains centrifugate in terminating;
Centrifugate is subjected to classified filtering using membrane filtration pattern, respectively peptide fragment I, the 2500- of molecular cut off > 5000Da The peptide fragment IV of the peptide fragment II of 5000Da, the peptide fragment III of 1000-2500Da and < 1000Da;
Each peptide fragment of retention is freeze-dried, the sea cucumber intestine ovum polypeptide powder of different peptide fragments is obtained.
2. preparation method according to claim 1, which is characterized in that the enzyme preparation of addition is by neutral proteinase, alkaline egg White enzyme and de- bitter food flavor enzyme composition.
3. preparation method according to claim 2, which is characterized in that the additive amount of the neutral proteinase of addition is degreasing sea Join the 1-4% of intestines ovum, the additive amount of the alkali protease of addition is the 2-6% of degreasing sea cucumber intestine ovum, the de- bitter food flavor enzyme of addition Additive amount be degreasing sea cucumber intestine ovum 0.5-1.5%.
4. preparation method according to claim 1, which is characterized in that the temperature of fermentor is 50-60 DEG C, and enzymolysis time is 2-7h。
5. preparation method according to claim 1, which is characterized in that the temperature of high-temperature inactivation is 90-95 DEG C, the duration For 15-25min.
6. the sea cucumber intestine ovum for the different peptide fragments that the preparation method of sea cucumber intestine ovum polypeptide powder obtains described in -5 according to claim 1 is more Gly-His-Lys.
7. the sea cucumber intestine ovum polypeptide powder of difference peptide fragment according to claim 6, which is characterized in that including molecular weight > The peptide fragment I of 5000Da, the peptide fragment II of 2500-5000Da, the peptide fragment III of 1000-2500Da and molecular weight < 1000Da peptide fragment IV The sea cucumber intestine ovum polypeptide powder of four peptide fragments.
8. the sea cucumber intestine ovum polypeptide powder of difference peptide fragment according to claim 7, which is characterized in that the peptide fragment IV of < 1000Da Account for 92% or more of total weight.
9. health care product or cosmetics, which is characterized in that more with the sea cucumber intestine ovum of < 1000Da peptide fragment according to claim 7 Gly-His-Lys are main component.
10. liquid or solid beverage, which is characterized in that with the sea cucumber intestine ovum of < 1000Da peptide fragment according to claim 7 Polypeptide powder is primary raw material.
CN201910407097.0A 2019-05-15 2019-05-15 The preparation method and gained sea cucumber intestine ovum polypeptide powder of sea cucumber intestine ovum polypeptide powder Pending CN110195090A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111592582A (en) * 2020-06-29 2020-08-28 中食都庆(山东)生物技术有限公司 Sea cucumber intestinal peptide and preparation method and application thereof
CN112176017A (en) * 2020-10-27 2021-01-05 威海市宇王集团海洋生物工程有限公司 Method for efficiently extracting protein peptide from sea cucumber intestines
CN113080467A (en) * 2021-05-14 2021-07-09 烟台大学 Oral liquid of red Jishen egg in icelandic
CN113142591A (en) * 2021-04-20 2021-07-23 烟台大学 Iceland red ginseng egg polypeptide and application thereof
CN113349356A (en) * 2021-06-17 2021-09-07 烟台大学 Iceland red-pole ginseng intestine egg nutritional jelly and preparation method thereof
CN113430047A (en) * 2021-06-24 2021-09-24 大连平岛天然产物科技有限公司 Method for extracting grease and peptide from sea cucumber eggs
CN114128880A (en) * 2021-11-08 2022-03-04 大连海宝生物技术有限公司 Female sea cucumber egg nutrient solution and preparation method thereof

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007153768A (en) * 2005-12-02 2007-06-21 Fuji Sangyo Kk Immunoameliorator and method for producing the same
CN101191139A (en) * 2007-04-28 2008-06-04 付学军 Integrated extraction technique for sea cucumber polypeptide and polysaccharide
CN102605031A (en) * 2012-04-10 2012-07-25 武汉普赛特膜技术循环利用有限公司 Method for comprehensively using viscera of sea cucumber
CN104356201A (en) * 2014-11-05 2015-02-18 福州大学 Holothurian antioxidative peptide
CN104356200A (en) * 2014-11-05 2015-02-18 福州大学 Anti-oxidative peptide and preparation method thereof
CN104402972A (en) * 2014-11-05 2015-03-11 福州大学 Sea cucumber antioxidant polypeptide and preparation method thereof
CN106497741A (en) * 2016-09-23 2017-03-15 山东省海洋资源与环境研究院 A kind of processing method of sea cucumber different tissues low molecular weight polypeptide wine
CN106889295A (en) * 2017-02-09 2017-06-27 赵德润 A kind of preparation method of live body sea stichopus japonicus active peptide
CN107853620A (en) * 2017-09-28 2018-03-30 大连豪翔生物酶工程有限公司 The technique of the integrated extraction of bioactive ingredients in a kind of sea cucumber intestine ovum

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007153768A (en) * 2005-12-02 2007-06-21 Fuji Sangyo Kk Immunoameliorator and method for producing the same
CN101191139A (en) * 2007-04-28 2008-06-04 付学军 Integrated extraction technique for sea cucumber polypeptide and polysaccharide
CN102605031A (en) * 2012-04-10 2012-07-25 武汉普赛特膜技术循环利用有限公司 Method for comprehensively using viscera of sea cucumber
CN104356201A (en) * 2014-11-05 2015-02-18 福州大学 Holothurian antioxidative peptide
CN104356200A (en) * 2014-11-05 2015-02-18 福州大学 Anti-oxidative peptide and preparation method thereof
CN104402972A (en) * 2014-11-05 2015-03-11 福州大学 Sea cucumber antioxidant polypeptide and preparation method thereof
CN106497741A (en) * 2016-09-23 2017-03-15 山东省海洋资源与环境研究院 A kind of processing method of sea cucumber different tissues low molecular weight polypeptide wine
CN106889295A (en) * 2017-02-09 2017-06-27 赵德润 A kind of preparation method of live body sea stichopus japonicus active peptide
CN107853620A (en) * 2017-09-28 2018-03-30 大连豪翔生物酶工程有限公司 The technique of the integrated extraction of bioactive ingredients in a kind of sea cucumber intestine ovum

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
赵晓玥: "海参肠、卵酶解物的制备工艺及性质研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111592582A (en) * 2020-06-29 2020-08-28 中食都庆(山东)生物技术有限公司 Sea cucumber intestinal peptide and preparation method and application thereof
CN112176017A (en) * 2020-10-27 2021-01-05 威海市宇王集团海洋生物工程有限公司 Method for efficiently extracting protein peptide from sea cucumber intestines
CN113142591A (en) * 2021-04-20 2021-07-23 烟台大学 Iceland red ginseng egg polypeptide and application thereof
CN113080467A (en) * 2021-05-14 2021-07-09 烟台大学 Oral liquid of red Jishen egg in icelandic
CN113349356A (en) * 2021-06-17 2021-09-07 烟台大学 Iceland red-pole ginseng intestine egg nutritional jelly and preparation method thereof
CN113430047A (en) * 2021-06-24 2021-09-24 大连平岛天然产物科技有限公司 Method for extracting grease and peptide from sea cucumber eggs
CN114128880A (en) * 2021-11-08 2022-03-04 大连海宝生物技术有限公司 Female sea cucumber egg nutrient solution and preparation method thereof

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