CN110195090A - The preparation method and gained sea cucumber intestine ovum polypeptide powder of sea cucumber intestine ovum polypeptide powder - Google Patents
The preparation method and gained sea cucumber intestine ovum polypeptide powder of sea cucumber intestine ovum polypeptide powder Download PDFInfo
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- CN110195090A CN110195090A CN201910407097.0A CN201910407097A CN110195090A CN 110195090 A CN110195090 A CN 110195090A CN 201910407097 A CN201910407097 A CN 201910407097A CN 110195090 A CN110195090 A CN 110195090A
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- 210000000936 intestine Anatomy 0.000 title claims abstract description 61
- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 60
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 60
- 241000251511 Holothuroidea Species 0.000 title claims abstract description 60
- 210000004681 ovum Anatomy 0.000 title claims abstract description 59
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 52
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 37
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 36
- 239000000843 powder Substances 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 102000007079 Peptide Fragments Human genes 0.000 claims abstract description 47
- 108010033276 Peptide Fragments Proteins 0.000 claims abstract description 47
- 102000004190 Enzymes Human genes 0.000 claims abstract description 26
- 108090000790 Enzymes Proteins 0.000 claims abstract description 26
- 230000002779 inactivation Effects 0.000 claims abstract description 7
- 230000007071 enzymatic hydrolysis Effects 0.000 claims abstract description 6
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract description 6
- 238000005374 membrane filtration Methods 0.000 claims abstract description 3
- 238000005238 degreasing Methods 0.000 claims description 20
- 239000000654 additive Substances 0.000 claims description 15
- 230000000996 additive effect Effects 0.000 claims description 15
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 9
- 239000000796 flavoring agent Substances 0.000 claims description 9
- 235000019634 flavors Nutrition 0.000 claims description 9
- 235000013305 food Nutrition 0.000 claims description 9
- 108091005804 Peptidases Proteins 0.000 claims description 8
- 239000004365 Protease Substances 0.000 claims description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 8
- 239000003513 alkali Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 235000019419 proteases Nutrition 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 4
- 230000009849 deactivation Effects 0.000 claims description 3
- 230000014759 maintenance of location Effects 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 235000013361 beverage Nutrition 0.000 claims description 2
- 239000002537 cosmetic Substances 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 230000036541 health Effects 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 claims 2
- 108010038983 glycyl-histidyl-lysine Proteins 0.000 claims 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims 1
- 235000014103 egg white Nutrition 0.000 claims 1
- 210000000969 egg white Anatomy 0.000 claims 1
- 235000013601 eggs Nutrition 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 12
- 230000007062 hydrolysis Effects 0.000 abstract description 7
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 7
- 238000009776 industrial production Methods 0.000 abstract description 3
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 235000019640 taste Nutrition 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
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- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 235000019636 bitter flavor Nutrition 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
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- 210000002249 digestive system Anatomy 0.000 description 1
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- 230000008092 positive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The present invention proposes the preparation method and gained sea cucumber intestine ovum polypeptide powder of a kind of sea cucumber intestine ovum polypeptide powder, belong to polypeptide and enzyme processing technique field, nutriment can be discharged into solution by the preparation method by the method for biological enzymolysis, obtain the peptide solution of degree of hydrolysis height, active peptide rich content, rational technology, strong operability, are easy to realize industrial production.The technical solution includes obtaining the sea cucumber intestine ovum polypeptide powder of different peptide fragments by fermentation enzymatic hydrolysis, high-temperature inactivation, classification membrane filtration pattern.During the deep development that the present invention can be applied to sea cucumber intestine ovum utilizes.
Description
Technical field
The invention belongs to the preparation method of polypeptide and enzyme processing technique field more particularly to a kind of sea cucumber intestine ovum polypeptide powder and
Gained sea cucumber intestine ovum polypeptide powder.
Background technique
Sea cucumber intestine ovum high-quality protein rich in and 8 kinds of amino acid needed by human and various trace elements, have compared with
Strong nourishing and strengthening Yin and Yang, improvement digestive system microcirculation, enhancing myocardial nutrition blood circulation flow, inhibition growth of tumour cell and expansion
Scattered effect.
However, it is improper due to traditional processing mode, so that sea cucumber intestine ovum is removed frequently as leftover bits and pieces, cause sea
Join the waste of resource.Sea cucumber intestine ovum polypeptide refers to the small-molecular peptides obtained after protease hydrolytic and separating-purifying, has a variety of function
Effect, such as lowering blood pressure and blood fat, antiatherosclerosis, antifatigue, antibacterial, improve immunity, anti-aging and anti-oxidant all
More physiological functions have other nutriment irreplaceable roles to human physiological functions.Since sea cucumber intestine ovum polypeptide has
The special physicochemical properties such as good dissolubility, stability, low viscosity, absorption easy to digest, no antigen, edible safety,
Therefore, sea cucumber intestine ovum polypeptide has higher biological value than other common cucumber products.
Modern biotechnology metabolism research discovery: the protein of human consumption is after gastral a variety of enzyme hydrolysis, more
It is directly to be absorbed in the form of low peptide, there is higher nutritive value and biological value.Enzyme hydrolysis method has obtained biologically active peptide
Through the main means for becoming industrialized production biologically active peptide.However, at present using enzymolysis process to sea cucumber intestine ovum at
When reason, not already available degree of hydrolysis is high, therefore how active peptide rich content biologically active peptide improves existing enzymatic hydrolysis work
Skill is particularly important the exploitation of sea cucumber intestine ovum polypeptide.
Summary of the invention
The present invention proposes the preparation method and gained sea cucumber intestine ovum polypeptide powder of a kind of sea cucumber intestine ovum polypeptide powder, the preparation method
Nutriment can be discharged into solution by the method for biological enzymolysis, it is molten to obtain degree of hydrolysis height, the peptide of active peptide rich content
Liquid, rational technology, strong operability, is easy to realize industrial production.
In order to achieve the above object, the present invention provides a kind of preparation methods of sea cucumber intestine ovum polypeptide powder, including following step
It is rapid:
After the degreasing sea cucumber intestine egg powder that pre-sifted is chosen is broken, enzyme preparation is added, persistently stirring is digested in fermentor；
Enzymatic hydrolysis makes enzyme complete deactivation using high-temperature inactivation, is then centrifuged, is centrifuged to enzymolysis liquid in terminating
Liquid；
The peptide fragment III of the peptide fragment I of molecular cut off > 5000Da, the peptide fragment II of 2500-5000Da, 1000-2500Da respectively
With the peptide fragment IV of < 1000Da；
Each peptide fragment of retention is freeze-dried, the sea cucumber intestine ovum polypeptide powder of different peptide fragments is obtained.
Preferably, the enzyme preparation being added is made of neutral proteinase, alkali protease and de- bitter food flavor enzyme.
In above-mentioned technical proposal, compatibility is carried out using above-mentioned enzyme, reason is that sea cucumber intestine ovum liquid pH7.2-8.3 omits alkali
Property, neutral proteinase and alkali protease combination are selected, enzymolysis efficiency and enzymatic activity can be effectively improved；And add de- bitter food flavor enzyme
It is then and to easily cause taste variation since low molecular weight peptide fragment content accounting is larger in product, and de- bitter food flavor enzyme can then have
The bitter taste of effect control peptide, to obtain that properties of product are higher, the better product of taste.
Preferably, the additive amount for the neutral proteinase being added is the 1-4% of degreasing sea cucumber intestine ovum, the basic protein of addition
The additive amount of enzyme is the 2-6% of degreasing sea cucumber intestine ovum, and the additive amount of the de- bitter food flavor enzyme of addition is the 0.5- of degreasing sea cucumber intestine ovum
1.5%.
Preferably, the temperature of fermentor is 50-60 DEG C, enzymolysis time 2-7h.
Preferably, the temperature of high-temperature inactivation is 90-95 DEG C, duration 15-25min.
The present invention provides a kind of, and the preparation method of sea cucumber intestine ovum polypeptide powder as described in any of the above-described technical solution obtains
Different peptide fragments sea cucumber intestine ovum polypeptide powder.
Preferably, including the peptide fragment I of molecular weight > 5000Da, the peptide fragment II of 2500-5000Da, 1000-2500Da
The sea cucumber intestine ovum polypeptide powder of IV 4 peptide fragments of peptide fragment of peptide fragment III and molecular weight < 1000Da.
Preferably, the peptide fragment IV of < 1000Da accounts for 92% of total weight or more.
The present invention also provides a kind of health care product or cosmetics, < 1000Da peptide fragment described in techniques described above scheme
Sea cucumber intestine ovum polypeptide powder is main component.
The present invention also provides a kind of liquid or solid beverage, < 1000Da peptide fragment described in techniques described above scheme
Sea cucumber intestine ovum polypeptide powder is primary raw material.
Compared with prior art, the advantages and positive effects of the present invention are:
The preparation method of sea cucumber intestine ovum polypeptide powder provided by the invention can be released nutriment by the method for biological enzymolysis
It is put into solution, obtains the peptide solution of degree of hydrolysis height, active peptide rich content, wherein can direct absorbed < 1000Da peptide fragment
Content is up to 92% or more.The preparation method rational technology, strong operability, are easy to realize industrial production.In addition, institute of the present invention
In the method final step of offer when obtaining the peptide solution of each peptide fragment by membrane filtration technique, sea cucumber intestine can be also effectively overcome
The high problem of ash content in ovum polypeptide.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Embodiment 1
Degreasing sea cucumber intestine ovum raw material is subjected to prescreening, it is desirable that raw material free from extraneous odour, impurity etc. crush it, it is desirable that
Then degreasing sea cucumber intestine ovum is put into fermentor, and adds corresponding enzyme according to the content of degreasing sea cucumber intestine ovum by partial size >=60 mesh
Preparation, persistently stirring carries out enzymatic hydrolysis 7h in 50 DEG C of fermentor；Wherein, the enzyme preparation being added includes neutral proteinase, alkali
Property protease, de- bitter flavor enzyme preparation, the additive amount for the neutral proteinase being added is the 1% of degreasing sea cucumber intestine ovum, addition
The additive amount of alkali protease is the 2% of degreasing sea cucumber intestine ovum, and the additive amount of the de- bitter food flavor enzyme of addition is degreasing sea cucumber intestine ovum
0.5%；
Enzymatic hydrolysis, in 90 DEG C of high-temperature inactivation 25min, makes enzyme complete deactivation, then enzymolysis liquid is centrifuged in terminating,
Obtain centrifugate；
By ceramic membrane equipment to centrifugate carry out classified filtering, respectively molecular cut off > 5000Da peptide fragment I,
The peptide fragment IV of the peptide fragment II of 2500-5000Da, the peptide fragment III of 1000-2500Da and < 1000Da；
Each peptide fragment of retention is freeze-dried in -40 DEG C, obtains the sea cucumber intestine ovum polypeptide powder of different peptide fragments.
Embodiment 2
Identical as 1 step of embodiment, difference place is: the temperature of fermentor is 55 DEG C, enzymolysis time 4.5h；High temperature
The temperature of inactivation is 92 DEG C, time 20min；The additive amount for the neutral proteinase being added is the 2% of degreasing sea cucumber intestine ovum, is added
The additive amount of the alkali protease entered is the 4% of degreasing sea cucumber intestine ovum, and the additive amount of the de- bitter food flavor enzyme of addition is degreasing sea cucumber
The 1.0% of intestines ovum.
Embodiment 3
Identical as 1 step of embodiment, difference place is: the temperature of fermentor is 60 DEG C, enzymolysis time 2h；High temperature goes out
Temperature living is 90 DEG C, time 15min；The additive amount for the neutral proteinase being added is the 4% of degreasing sea cucumber intestine ovum, is added
The additive amount of alkali protease be the 6% of degreasing sea cucumber intestine ovum, the additive amount of the de- bitter food flavor enzyme of addition is degreasing sea cucumber intestine
The 1.5% of ovum.
The sea cucumber intestine ovum polypeptide molecular weight testing result of different peptide fragments prepared by embodiment 1-3 is as shown in table 1.
1 sea cucumber intestine ovum polypeptide molecular weight testing result of table
From the data in table 1, it can be seen that preparation method provided in an embodiment of the present invention can digest to obtain degree of hydrolysis height, active peptide content
The peptide solution of multiple peptide fragments abundant, wherein divided by molecular weight, the peptide fragment content of 1000 molecular weight of < up to 92% or more,
And there is absolute predominance in the peptide fragment being distributed at four.The peptide fragment of 1000 molecular weight of < digested in above-described embodiment 1-3
After tested, up to 98%, absorptivity can directly be absorbed by the body dissolubility up to 99.6% or more, have higher
Nutritive value and biological value.
Claims (10)
1. the preparation method of sea cucumber intestine ovum polypeptide powder, which comprises the following steps:
After the degreasing sea cucumber intestine egg powder that pre-sifted is chosen is broken, enzyme preparation is added, persistently stirring is digested in fermentor；
Enzymatic hydrolysis makes enzyme complete deactivation using high-temperature inactivation, is then centrifuged to enzymolysis liquid, obtains centrifugate in terminating；
Centrifugate is subjected to classified filtering using membrane filtration pattern, respectively peptide fragment I, the 2500- of molecular cut off > 5000Da
The peptide fragment IV of the peptide fragment II of 5000Da, the peptide fragment III of 1000-2500Da and < 1000Da；
Each peptide fragment of retention is freeze-dried, the sea cucumber intestine ovum polypeptide powder of different peptide fragments is obtained.
2. preparation method according to claim 1, which is characterized in that the enzyme preparation of addition is by neutral proteinase, alkaline egg
White enzyme and de- bitter food flavor enzyme composition.
3. preparation method according to claim 2, which is characterized in that the additive amount of the neutral proteinase of addition is degreasing sea
Join the 1-4% of intestines ovum, the additive amount of the alkali protease of addition is the 2-6% of degreasing sea cucumber intestine ovum, the de- bitter food flavor enzyme of addition
Additive amount be degreasing sea cucumber intestine ovum 0.5-1.5%.
4. preparation method according to claim 1, which is characterized in that the temperature of fermentor is 50-60 DEG C, and enzymolysis time is
2-7h。
5. preparation method according to claim 1, which is characterized in that the temperature of high-temperature inactivation is 90-95 DEG C, the duration
For 15-25min.
6. the sea cucumber intestine ovum for the different peptide fragments that the preparation method of sea cucumber intestine ovum polypeptide powder obtains described in -5 according to claim 1 is more
Gly-His-Lys.
7. the sea cucumber intestine ovum polypeptide powder of difference peptide fragment according to claim 6, which is characterized in that including molecular weight >
The peptide fragment I of 5000Da, the peptide fragment II of 2500-5000Da, the peptide fragment III of 1000-2500Da and molecular weight < 1000Da peptide fragment IV
The sea cucumber intestine ovum polypeptide powder of four peptide fragments.
8. the sea cucumber intestine ovum polypeptide powder of difference peptide fragment according to claim 7, which is characterized in that the peptide fragment IV of < 1000Da
Account for 92% or more of total weight.
9. health care product or cosmetics, which is characterized in that more with the sea cucumber intestine ovum of < 1000Da peptide fragment according to claim 7
Gly-His-Lys are main component.
10. liquid or solid beverage, which is characterized in that with the sea cucumber intestine ovum of < 1000Da peptide fragment according to claim 7
Polypeptide powder is primary raw material.
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| CN111592582A (en) * | 2020-06-29 | 2020-08-28 | 中食都庆（山东）生物技术有限公司 | Sea cucumber intestinal peptide and preparation method and application thereof |
| CN112176017A (en) * | 2020-10-27 | 2021-01-05 | 威海市宇王集团海洋生物工程有限公司 | Method for efficiently extracting protein peptide from sea cucumber intestines |
| CN113080467A (en) * | 2021-05-14 | 2021-07-09 | 烟台大学 | Oral liquid of red Jishen egg in icelandic |
| CN113142591A (en) * | 2021-04-20 | 2021-07-23 | 烟台大学 | Iceland red ginseng egg polypeptide and application thereof |
| CN113349356A (en) * | 2021-06-17 | 2021-09-07 | 烟台大学 | Iceland red-pole ginseng intestine egg nutritional jelly and preparation method thereof |
| CN113430047A (en) * | 2021-06-24 | 2021-09-24 | 大连平岛天然产物科技有限公司 | Method for extracting grease and peptide from sea cucumber eggs |
| CN114128880A (en) * | 2021-11-08 | 2022-03-04 | 大连海宝生物技术有限公司 | Female sea cucumber egg nutrient solution and preparation method thereof |
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| CN113080467A (en) * | 2021-05-14 | 2021-07-09 | 烟台大学 | Oral liquid of red Jishen egg in icelandic |
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| CN113430047A (en) * | 2021-06-24 | 2021-09-24 | 大连平岛天然产物科技有限公司 | Method for extracting grease and peptide from sea cucumber eggs |
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