CN105192244A - Method for preparing snakegourd fruit seed polypeptides - Google Patents
Method for preparing snakegourd fruit seed polypeptides Download PDFInfo
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- CN105192244A CN105192244A CN201510633883.4A CN201510633883A CN105192244A CN 105192244 A CN105192244 A CN 105192244A CN 201510633883 A CN201510633883 A CN 201510633883A CN 105192244 A CN105192244 A CN 105192244A
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Abstract
The invention discloses a method for preparing snakegourd fruit seed polypeptides, which comprises the steps: (1) degreasing: snakegourd fruit seeds are degreased by adopting a low temperature cold-pressed technique; (2) crushing and dissolving: a snakegourd fruit seed suspension is prepared; (3) homogeneous processing: the snakegourd fruit seed suspension is treated through double-stage homogenization; (4) enzymolysis: a snakegourd fruit seed mixing solution is obtained through adopting complex phosphoesterasum; (5) centrifugal filtration; (6) high temperature setrilization; (7) ultrafiltration refining; and (8) concentration and desiccation. The method for preparing the snakegourd fruit seed polypeptides has the beneficle effects: the snakegourd fruit seed polypeptides have the advantages that the snakegourd fruit seed polypeptide reduces cholesterol and blood, promotes fat metabolism, resists fatigue, enhances human immunity and adjusts human body physiology and the like, and has wide development and application prospect in the fields of food industry, health food and medicine and the like.
Description
Technical field
The present invention relates to a kind of health food manufacture field, specifically a kind of preparation method of Snakegourd Fruit seed polypeptide.
Background technology
Snakegourd Fruit, the perennial liana of Curcurbitaceae, effects such as being a kind of famous and precious Chinese medicine, recording according to TCM Literature: PERICARPIUM TRICHOSANTHIS, seed, root all can be used as medicine, there is moistening lung for removing phlegm, fall fiery cough-relieving, relieving chest stiffness to dissipate mass, detumescence detoxification, relax bowel.Snakegourd Fruit seed contains unrighted acid 16.8%, protein 5.46%, and containing 17 seed amino acids, three paste saponins, 16 kinds of trace elements such as multivitamin and calcium, iron, zinc, selenium.Especially content of vitamin E abundant (100 grams of Semen Trichosanthis are containing vitamin E 49 milligrams), can skin nutrition, and smooth away wrinkles, conditioning human body, delays senility.Edible Snakegourd Fruit seed, can also expand heart coronary artery, increases coronary flow effect.
Polypeptide has good nutritive peculiarity, absorption easy to digest, especially some micromolecular peptide class, can not only rapidly for body provides energy, and there is unique processing characteristics, simultaneously, Snakegourd Fruit seed polypeptide has and reduces cholesterol, hypotensive and promote fat metabolism, antifatigue, enhancing body immunity, regulate effect advantage such as human physiological functions, but, nutritional labeling in Snakegourd Fruit seed can not directly be absorbed by the body, this makes Snakegourd Fruit seed Middle nutrition composition energy directly absorbed by the body with regard to needing by suitable method, thus plays the nutritive value of Snakegourd Fruit seed.
Summary of the invention
The present invention aims to provide a kind of preparation method of Snakegourd Fruit seed polypeptide, solve the degradation problem of high-fat Snakegourd Fruit seed protein, can, by the various nutritional labeling degradeds in degreasing Snakegourd Fruit seed, it be made to generate that directly can absorb for human body, nutritious Snakegourd Fruit seed polypeptide powder.
The invention provides following technical scheme:
A preparation method for Snakegourd Fruit seed polypeptide, comprises the following steps:
(1) degreasing: adopt low-temperature cold-squeezing to carry out ungrease treatment to Snakegourd Fruit seed;
(2) pulverize dissolving: the degreased sunflower seeds obtained after pulverizer is pulverized by Snakegourd Fruit seed is obtained Snakegourd Fruit seed cake powder particle after 40 mesh sieves, then Snakegourd Fruit seed crushed material is mixed with the ratio uniform of water by weight 1:6, obtained Snakegourd Fruit seed suspension;
(3) homogeneous process: obtained Snakegourd Fruit seed suspension homogenizer is carried out double-stage homogenization process, obtain Snakegourd Fruit seed mixed liquor, one-level homogenization pressure 45-60MPa, double-stage homogenization pressure 15-25MPa, temperature 80 DEG C, emulsion vacuum outgas after homogeneous, vacuum 0.10-0.20MPa, degassed duration 4-8 minute;
(4) enzymolysis: the Snakegourd Fruit seed mixed liquor obtained with complex enzyme process, complex enzyme addition is the 0.05%-0.15% of Snakegourd Fruit seed mixing liquid measure, be temperature conditions is 50 DEG C, pH value 7.0 ~ 8.5, be hydrolyzed 4 hours at enzymatic hydrolysis condition, obtains Snakegourd Fruit seed enzymolysis liquid;
(5) centrifugal filtration: put centrifugal in centrifuges by the Snakegourd Fruit seed enzymolysis liquid obtained, rotating speed is 500r/min, and the time is 10min, then filters with flame filter press, obtains filtered fluid;
(6) high-temperature sterilization: be rapidly heated the temperature of Snakegourd Fruit seed seed filtered fluid to 50 ~ 55 DEG C, and be incubated 10min, then carries out solid-liquor separation removing insoluble solid, obtained Snakegourd Fruit seed polypeptide liquid;
(7) ultrafiltration refining: the Snakegourd Fruit seed liquid polypeptide milipore filter obtained after high-temperature sterilization is carried out classification separation by molecular weight, obtains different molecular weight Snakegourd Fruit seed polypeptide solution;
(8) concentrate drying: adopt Vacuum Concentration to obtain Snakegourd Fruit seed polypeptide concentrate, the moisture of Snakegourd Fruit seed polypeptide concentrate is 50 ~ 60%, then obtains Snakegourd Fruit seed polypeptide powder by spray-dired mode.
Described complex enzyme is papain and tryptic mixing composition, and papain and tryptic volume ratio are 1:1.
Described polypeptide is powder, can be processed into the forms such as particle, tablet, capsule, oral liquid further.
Compared with prior art, the invention has the beneficial effects as follows: polypeptide has good nutritive peculiarity, absorption easy to digest, especially some micromolecular peptide class, can not only rapidly for body provides energy, and there is unique processing characteristics, simultaneously, Snakegourd Fruit seed polypeptide has and reduces cholesterol, hypotensive and promote fat metabolism, antifatigue, enhancing body immunity, regulate effect advantage such as human physiological functions to have wide development prospect in food industry, health food and medicine and other fields.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
A preparation method for Snakegourd Fruit seed polypeptide, comprises the following steps:
(1) degreasing: adopt low-temperature cold-squeezing to carry out ungrease treatment to Snakegourd Fruit seed;
(2) pulverize dissolving: the degreased sunflower seeds obtained after pulverizer is pulverized by Snakegourd Fruit seed is obtained Snakegourd Fruit seed cake powder particle after 40 mesh sieves, then Snakegourd Fruit seed crushed material is mixed with the ratio uniform of water by weight 1:6, obtained Snakegourd Fruit seed suspension;
(3) homogeneous process: obtained Snakegourd Fruit seed suspension homogenizer is carried out double-stage homogenization process, obtain Snakegourd Fruit seed mixed liquor, one-level homogenization pressure 45-60MPa, double-stage homogenization pressure 15-25MPa, temperature 80 DEG C, emulsion vacuum outgas after homogeneous, vacuum 0.10-0.20MPa, degassed duration 4-8 minute;
(4) enzymolysis: the Snakegourd Fruit seed mixed liquor obtained with complex enzyme process, complex enzyme addition is the 0.05%-0.15% of Snakegourd Fruit seed mixing liquid measure, be temperature conditions is 50 DEG C, pH value 7.0 ~ 8.5, be hydrolyzed 4 hours at enzymatic hydrolysis condition, obtains Snakegourd Fruit seed enzymolysis liquid;
(5) centrifugal filtration: put centrifugal in centrifuges by the Snakegourd Fruit seed enzymolysis liquid obtained, rotating speed is 500r/min, and the time is 10min, then filters with flame filter press, obtains filtered fluid;
(6) high-temperature sterilization: be rapidly heated the temperature of Snakegourd Fruit seed seed filtered fluid to 50 ~ 55 DEG C, and be incubated 10min, then carries out solid-liquor separation removing insoluble solid, obtained Snakegourd Fruit seed polypeptide liquid;
(7) ultrafiltration refining: the Snakegourd Fruit seed liquid polypeptide milipore filter obtained after high-temperature sterilization is carried out classification separation by molecular weight, obtains different molecular weight Snakegourd Fruit seed polypeptide solution;
(8) concentrate drying: adopt Vacuum Concentration to obtain Snakegourd Fruit seed polypeptide concentrate, the moisture of Snakegourd Fruit seed polypeptide concentrate is 50 ~ 60%, then obtains Snakegourd Fruit seed polypeptide powder by spray-dired mode.
Described complex enzyme is papain and tryptic mixing composition, and papain and tryptic volume ratio are 1:1.
Described polypeptide is powder, can be processed into the forms such as particle, tablet, capsule, oral liquid further.
Claims (3)
1. a preparation method for Snakegourd Fruit seed polypeptide, is characterized in that, comprises the following steps:
(1) degreasing: adopt low-temperature cold-squeezing to carry out ungrease treatment to Snakegourd Fruit seed;
(2) pulverize dissolving: the degreased sunflower seeds obtained after pulverizer is pulverized by Snakegourd Fruit seed is obtained Snakegourd Fruit seed cake powder particle after 40 mesh sieves, then Snakegourd Fruit seed crushed material is mixed with the ratio uniform of water by weight 1:6, obtained Snakegourd Fruit seed suspension;
(3) homogeneous process: obtained Snakegourd Fruit seed suspension homogenizer is carried out double-stage homogenization process, obtain Snakegourd Fruit seed mixed liquor, one-level homogenization pressure 45-60MPa, double-stage homogenization pressure 15-25MPa, temperature 80 DEG C, emulsion vacuum outgas after homogeneous, vacuum 0.10-0.20MPa, degassed duration 4-8 minute;
(4) enzymolysis: the Snakegourd Fruit seed mixed liquor obtained with complex enzyme process, complex enzyme addition is the 0.05%-0.15% of Snakegourd Fruit seed mixing liquid measure, be temperature conditions is 50 DEG C, pH value 7.0 ~ 8.5, be hydrolyzed 4 hours at enzymatic hydrolysis condition, obtains Snakegourd Fruit seed enzymolysis liquid;
(5) centrifugal filtration: put centrifugal in centrifuges by the Snakegourd Fruit seed enzymolysis liquid obtained, rotating speed is 500r/min, and the time is 10min, then filters with flame filter press, obtains filtered fluid;
(6) high-temperature sterilization: be rapidly heated the temperature of Snakegourd Fruit seed seed filtered fluid to 50 ~ 55 DEG C, and be incubated 10min, then carries out solid-liquor separation removing insoluble solid, obtained Snakegourd Fruit seed polypeptide liquid;
(7) ultrafiltration refining: the Snakegourd Fruit seed liquid polypeptide milipore filter obtained after high-temperature sterilization is carried out classification separation by molecular weight, obtains different molecular weight Snakegourd Fruit seed polypeptide solution;
(8) concentrate drying: adopt Vacuum Concentration to obtain Snakegourd Fruit seed polypeptide concentrate, the moisture of Snakegourd Fruit seed polypeptide concentrate is 50 ~ 60%, then obtains Snakegourd Fruit seed polypeptide powder by spray-dired mode.
2. the preparation method of a kind of Snakegourd Fruit seed polypeptide according to claim 1, is characterized in that, described complex enzyme is papain and tryptic mixing composition, and papain and tryptic volume ratio are 1:1.
3. the preparation method of a kind of Snakegourd Fruit seed polypeptide according to claim 1, it is characterized in that, described polypeptide is powder, can be processed into the forms such as particle, tablet, capsule, oral liquid further.
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| CN201510633883.4A CN105192244A (en) | 2015-09-29 | 2015-09-29 | Method for preparing snakegourd fruit seed polypeptides |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105995269A (en) * | 2016-05-24 | 2016-10-12 | 潜山县有余瓜蒌开发有限责任公司 | Degreased trichosanthes kirilowii maxim composite protein beverage and preparation process thereof |
| CN112708651A (en) * | 2020-12-30 | 2021-04-27 | 杭州娃哈哈科技有限公司 | Snakegourd fruit protein powder and polypeptide powder as well as preparation methods and applications thereof |
| CN116671638A (en) * | 2023-06-15 | 2023-09-01 | 江西维莱营健高科有限公司 | Casein calcium tablet and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101589761A (en) * | 2008-05-29 | 2009-12-02 | 中国人民解放军总后勤部军需装备研究所 | A kind of preparation method of industrial hemp seed antioxidant peptide and application |
| CN102630804A (en) * | 2012-04-27 | 2012-08-15 | 湖北智慧果林业科技有限公司 | Production process of walnut peptide powder |
| CN103250865A (en) * | 2013-03-11 | 2013-08-21 | 武汉工业学院 | Preparation method of peanut polypeptide |
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2015
- 2015-09-29 CN CN201510633883.4A patent/CN105192244A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101589761A (en) * | 2008-05-29 | 2009-12-02 | 中国人民解放军总后勤部军需装备研究所 | A kind of preparation method of industrial hemp seed antioxidant peptide and application |
| CN102630804A (en) * | 2012-04-27 | 2012-08-15 | 湖北智慧果林业科技有限公司 | Production process of walnut peptide powder |
| CN103250865A (en) * | 2013-03-11 | 2013-08-21 | 武汉工业学院 | Preparation method of peanut polypeptide |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105995269A (en) * | 2016-05-24 | 2016-10-12 | 潜山县有余瓜蒌开发有限责任公司 | Degreased trichosanthes kirilowii maxim composite protein beverage and preparation process thereof |
| CN112708651A (en) * | 2020-12-30 | 2021-04-27 | 杭州娃哈哈科技有限公司 | Snakegourd fruit protein powder and polypeptide powder as well as preparation methods and applications thereof |
| CN116671638A (en) * | 2023-06-15 | 2023-09-01 | 江西维莱营健高科有限公司 | Casein calcium tablet and preparation method thereof |
| CN116671638B (en) * | 2023-06-15 | 2024-03-22 | 江西维莱营健高科有限公司 | Casein calcium tablet and preparation method thereof |
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