CN108546620A - A kind of deer ginseng small-molecular peptides nourish wine and preparation method thereof - Google Patents
A kind of deer ginseng small-molecular peptides nourish wine and preparation method thereof Download PDFInfo
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Abstract
A kind of deer ginseng small-molecular peptides nourish wine and preparation method thereof, it is related to nourishing food field, the nourishing wine is using deer whip, deer blood, deer's tail as primary raw material, small-molecular peptides are prepared through complex enzyme hydrolysis technology, and combine traditional Chinese medicine compatibility theory, it with integration of drinking and medicinal herbs Chinese medicine reasonable formula, extracts through bioactive ingredients, is formulated with white spirit mixed, hook tune.The small-molecular peptides health liquor includes：Base liquor 1000mL；0.05~0.3g of small molecule Gly-His-Lys；3~10g of Chinese medical extract；Small molecule Gly-His-Lys include：50~70 parts of deer whip, 10~30 parts of deer blood, 10~30 parts of deer's tail；Chinese medical extract includes：1~5 part of ginseng, 0.1~1 part of eucommia Bark male flower, 10~30 parts of raspberry, 20~40 parts of the fruit of Chinese wolfberry, 10~20 parts of jujube, 5~10 parts of Radix Glycyrrhizae.The nourishing wine pure in mouth feel, do not precipitate, stability it is good.Used deer blood, deer whip, deer's tail micromolecule polypeptide are easily absorbed by the body, and nutritive value is high.
Description
Technical field
The present invention relates to nourishing food technical fields, and in particular to a kind of deer ginseng small-molecular peptides nourish wine and preparation method thereof.
Background technology
Absorption of human body protein is carried out by the form of peptide.Protein is digested by digestive ferment in enteron aisle, small point
Sub- peptide can completely be absorbed, and into blood circulation system, and entered in cell with original shape by osmosis and inhaled by cell
It receives and utilizes.Food-borne peptide is using edible protein as raw material, by novel proteolytic product made of enzymolysis, separation, purifying etc..
Peptide of the relative molecular weight between 50~1000Da is known as small-molecular peptides, peptide of the relative molecular weight between 10000~5000Da
Referred to as big peptide.In recent years, domestic and international researcher detaches from different food protein enzymolysis products and identifies with various
The peptide of bioactivity, such as anti-oxidation peptide, Antihypertensive Peptides, antithrombotic peptide, lipid-loweringing peptide, opioid peptides, immunomodulatory peptides, antibacterial peptide.With
Fresh and alive silkworm chrysalis, which is the total nutrient silkworm chrysalis peptide that Raw material processing obtains, to be had reducing blood lipid, prevents cardiovascular and cerebrovascular disease, improve immunity, is anti-
Fatigue, anti-aging and other effects.Using new fresh sea cucumber or dry product sea cucumber as raw material, the sea cucumber peptide processed has antitumor, drop
Blood pressure and other effects.There is anticancer from the active pentapeptide of the defatted rice bran of thermal stability, weight-reducing, prevent the works such as senile dementia
With.Food-borne peptide is safe, it is active it is strong, dissolubility is good, absorptions is fast, utilization rate is high, and with extensive physiological regulatory action,
It is the function factor of great development prospect.
The peptide wine being modulated by adding protein peptides plays preferably improving immunity of organisms, functions of intestines and stomach etc.
Health-care effect, drink in right amount for a long time can strengthen immunity, adjust endocrine, protection liver, improve memory, is anti-aging, disease-resistant
Poison infection.Peptide biological activity in peptide wine is high, has good dissolubility, absorbs fast, utilization rate height in vivo, gives full play to battalion
Support healthcare function.
Invention content
Nourish wine and preparation method thereof the object of the present invention is to provide a kind of deer ginseng small-molecular peptides, the nourishing wine with deer whip,
Deer blood, deer's tail are primary raw material, prepare small-molecular peptides through complex enzyme hydrolysis technology, and combine traditional Chinese medicine compatibility theory, same with medicine food
Source Chinese medicine reasonable formula, is extracted through bioactive ingredients, is formulated with white spirit mixed, hook tune.
The present invention is that technical scheme applied to solve the technical problem is as follows：
A kind of deer ginseng small-molecular peptides of the present invention nourish wine, including：
Base liquor 1000mL；
0.05~0.3g of small molecule Gly-His-Lys；
3~10g of Chinese medical extract；
0.1~0.3g of white sugar；
0.1~0.3g of honey；
The small molecule Gly-His-Lys include according to parts by weight：50~70 parts of deer whip, 10~30 parts of deer blood, deer's tail 10~30
Part；
The Chinese medical extract includes according to parts by weight：1~5 part of ginseng, 0.1~1 part of eucommia Bark male flower, raspberry 10~
30 parts, 20~40 parts of the fruit of Chinese wolfberry, 10~20 parts of jujube, 5~10 parts of Radix Glycyrrhizae.
The present invention also provides the preparation methods that above-mentioned deer ginseng small-molecular peptides nourish wine, include the following steps：
Step 1: enzyme process prepares small molecule Gly-His-Lys
By deer whip, deer blood, deer's tail respectively after crushing, being sieved, the distilled water mixing of 10~20 times of weight is added, in gained
Papain, compound protease, flavor protease are sequentially added in mixture and carries out enzyme digestion reaction, obtain complex enzyme hydrolysis liquid,
Small-molecular peptides concentrate is obtained after coarse filtration, ultrafiltration, then obtains small molecule Gly-His-Lys after nanofiltration desalination, drying；
Step 2: preparing Chinese medical extract
Ginseng, eucommia Bark male flower, raspberry, the fruit of Chinese wolfberry, jujube, Radix Glycyrrhizae are uniformly mixed after crushing, using high hydrostatic pressure extraction,
Ethyl alcohol is recovered under reduced pressure, Chinese medical extract is obtained after freeze-dried；
Step 3: allotment
Chinese medical extract obtained by small molecule Gly-His-Lys and step 2 obtained by step 1 is added in base liquor, adds water decreasing concentration, mixing
White sugar and honey are added afterwards, nourish wine after filtering up to deer ginseng small-molecular peptides.
It is further comprising the steps of before carrying out step 1 as preferred embodiment：Deer whip, deer blood, deer's tail is former
Material is ground into the coarse powder of 100~300 mesh after drying.
As preferred embodiment, in step 1, the additive amount of the papain is the 0.1 of mixture gross mass
~0.3%；The additive amount of the compound protease is the 0.1~0.3% of mixture gross mass；The addition of the flavor protease
Amount is the 0.1~0.3% of mixture gross mass.
As preferred embodiment, in step 1, the papain enzymolysis reaction condition is：50~70 DEG C of enzymolysis
6~8 hours, 100 DEG C of enzyme deactivation 5min；The compound protease reaction conditions are：50~70 DEG C digest 6~8 hours, 100
DEG C enzyme deactivation 5min；The flavor protease reaction conditions are：50~70 DEG C digest 6~8 hours, 100 DEG C of enzyme deactivation 5min.
As preferred embodiment, in step 2, extraction conditions is：Solid-to-liquid ratio is 1:(5~10), temperature are 20~40
DEG C, ultra high pressure is 200~500MPa, and extraction time is 25~50min, and extraction time is 1~2 time.
As preferred embodiment, in step 2, extractant uses the aqueous solution of ethyl alcohol, the volume fraction of ethyl alcohol to be
40~70%.
As preferred embodiment, in step 3, when allotment, per in 1000mL base liquors, the small molecule Gly-His-Lys plus
It is 0.05~0.3g to enter amount, and the addition of the Chinese medical extract is 3~10g, and the addition of the white sugar is 0.1~0.3g,
The addition of the honey is 0.1~0.3g.
As preferred embodiment, in step 3, the volume fraction that distilled water decreasing concentration is added to ethyl alcohol is 30~50%.
The beneficial effects of the invention are as follows：
(1) deer blood, deer whip, the deer's tail micromolecule polypeptide added in the present invention is made by digesting ultrafiltration, is easily inhaled by human body
It receives, nutritive value higher.
(2) deer blood of the invention, deer whip, deer's tail deer ginseng small-molecular peptides nourishing wine have pure in mouth feel, do not precipitate, stability
Good feature can give full play to plurality of health care functions that deer blood, deer whip, deer's tail active constituent have and other Chinese medicines
Reasonable compatibility complements each other, and improves functional.
(3) in preparation method of the invention, traditional Chinese medicine extraction uses normal-temperature superhigh pressure abstraction technique, and skill is extracted instead of tradition
Simply immersing in art or the method for directly adding traditional Chinese medicine powder, can fully ensure that the activity of raw material active ingredient, and significantly
Improve recovery rate.
Specific implementation mode
A kind of deer ginseng small-molecular peptides of the present invention nourish wine, including：
Base liquor 1000mL；
0.05~0.3g of small molecule Gly-His-Lys；
3~10g of Chinese medical extract；
0.1~0.3g of white sugar；
0.1~0.3g of honey；
Small molecule Gly-His-Lys include according to parts by weight：50~70 parts of deer whip, 10~30 parts of deer blood, 10~30 parts of deer's tail；
Chinese medical extract includes according to parts by weight：1~5 part of ginseng, 0.1~1 part of eucommia Bark male flower, raspberry 10~30
Part, 20~40 parts of the fruit of Chinese wolfberry, 10~20 parts of jujube, 5~10 parts of Radix Glycyrrhizae.
The present invention also provides the preparation methods that above-mentioned deer ginseng small-molecular peptides nourish wine, wherein involved each raw material adds
Dosage includes the following steps according to above-mentioned progress, the preparation method：
Step 1: enzyme process prepares small molecule Gly-His-Lys
According to ratio requirement, deer whip, deer blood, deer's tail raw material are ground into 100~300 mesh after drying first and (specifically may be used
For the mesh of 100 mesh~200,100 mesh or 200 mesh) coarse powder, by deer whip, deer blood, deer's tail respectively after crushing, being sieved, addition 10~
The distilled water mixing of 20 times of weight, in the resulting mixture, sequentially adds the Papain of mixture gross mass 0.1~0.3%
Enzyme is digested：50~70 DEG C digest 6~8 hours, 100 DEG C of enzyme deactivation 5min；Add mixture gross mass 0.1~0.3%
Compound protease is digested：50~70 DEG C digest 6~8 hours, 100 DEG C of enzyme deactivation 5min；It is eventually adding mixture gross mass 0.1
~0.3% flavor protease is digested：50~70 DEG C digest 6~8 hours, 100 DEG C of enzyme deactivation 5min, obtain complex enzyme hydrolysis
Liquid obtains small-molecular peptides concentrate after coarse filtration, ultrafiltration, then is obtained after nanofiltration desalination, vacuum freeze drying or spray drying
Obtain small molecule Gly-His-Lys.
In above-mentioned zymolysis technique, select papain, compound protease, flavor protease to deer whip, deer blood, deer's tail into
Row enzymolysis, using biological enzyme high molecular weight protein resolve into small-molecular peptides aminoacid ingredient, have reaction condition mild, anti-
Answer the advantages that efficient.High molecular weight protein is more easy to be absorbed by the body after being hydrolyzed into small-molecular peptides, nutritive value higher.
Step 2: preparing Chinese medical extract
According to proportioning, by ginseng (5 years or 4 years artificial growth sun-dried ginsengs), eucommia Bark male flower, raspberry, the fruit of Chinese wolfberry, big
Jujube, Radix Glycyrrhizae are uniformly mixed after crushing, and using high hydrostatic pressure extraction, extractant uses the aqueous solution of ethyl alcohol, the aqueous solution of the ethyl alcohol
Can be edible alcohol, the volume fraction of ethyl alcohol is 40~70% in the edible alcohol, and extraction conditions is：Solid-to-liquid ratio is 1:(5~
10), temperature is 20~40 DEG C, and ultra high pressure is 200~500MPa, and extraction time is 25~50min, and extraction time is 1~2
It is secondary；Ethyl alcohol is recovered under reduced pressure, Chinese medical extract is obtained after freeze-dried.
Using high hydrostatic pressure extraction technology extract extraction Chinese medicine, have extraction time is short, solvent consumption is few, extraction efficiency is high and
The advantages such as Extracting temperature is low, can effectively extract the active ingredient in Chinese medicine, while can also fully ensure that the work of material effective component
Property.
Step 3: allotment
According to proportioning, Chinese medical extract obtained by small molecule Gly-His-Lys and step 2 obtained by step 1 is added in base liquor, often
In 1000mL base liquors, the addition of small molecule Gly-His-Lys is 0.05~0.3g, and the addition of Chinese medical extract is 3~10g；Add distillation
The volume fraction of water decreasing concentration to ethyl alcohol is 30~50%, and 0.1~0.3g white sugar and 0.1~0.3g honey are added after mixing well,
After filtering wine is nourished up to deer ginseng small-molecular peptides.
A kind of deer ginseng small-molecular peptides of the present invention nourish the preparation method of wine, and deer whip deer blood deer's tail tail is made by enzymatic isolation method
Micromolecule polypeptide ensures after being added to base liquor, pure in mouth feel, do not precipitate, stability it is good.
Prepare embodiment 1
The preparation process that the deer ginseng small-molecular peptides of the present invention nourish wine is as follows：
(1) enzyme process prepares small molecule Gly-His-Lys
60 parts of deer whips, 20 parts of deer blood, 20 parts of deer's tails are added into 15 times of weights through crushing, after 100 mesh fine powder standard screens excessively respectively
The distilled water of amount mixes, and in the resulting mixture, the papain for sequentially adding mixture gross mass 0.3% is digested：
60 DEG C digest 7 hours, 100 DEG C of enzyme deactivation 5min；The compound protease for adding mixture gross mass 0.3% is digested：60℃
Enzymolysis 7 hours, 100 DEG C of enzyme deactivation 5min；The flavor protease for being eventually adding mixture gross mass 0.3% is digested：60 DEG C of enzymes
Solution 7 hours, 100 DEG C of enzyme deactivation 5min obtains complex enzyme hydrolysis liquid, is obtained after coarse filtration, the ultrafiltration membrane ultrafiltration of molecular cut off 1KDa
Small-molecular peptides concentrate is obtained, then small molecule Gly-His-Lys are obtained after nanofiltration desalination, vacuum freeze drying or spray drying.
(2) Chinese medical extract is prepared
By 2 parts of ginsengs (5 years artificial growth sun-dried ginsengs), 1 part of eucommia Bark male flower, 30 portions of raspberries, 40 portions of fruits of Chinese wolfberry, 20 parts
Jujube, 7 portions of Radix Glycyrrhizaes are uniformly mixed after crushing, and using high hydrostatic pressure extraction, extraction conditions is：The ethyl alcohol that extractant is 50% is water-soluble
Liquid, solid-to-liquid ratio 1:10, temperature is 30 DEG C, ultra high pressure 300MPa, extracts 40min, is extracted 2 times；Ethyl alcohol is recovered under reduced pressure,
Chinese medical extract is obtained after freeze-dried.
(3) it allocates
Chinese medical extract obtained by small molecule Gly-His-Lys and step 2 obtained by step 1 is added in base liquor, per 1000mL base liquors
In, the addition of small molecule Gly-His-Lys is 0.08g, and the addition of Chinese medical extract is 7g；Add distilled water decreasing concentration to the volume of ethyl alcohol
Score is 35~42%, and white sugar 1g and honey 1g is added after mixing well, and nourishes wine after filtering up to deer ginseng small-molecular peptides.
Prepare embodiment 2
The preparation process that the deer ginseng small-molecular peptides of the present invention nourish wine is as follows：
(1) enzyme process prepares small molecule Gly-His-Lys
50 parts of deer whips, 10 parts of deer blood, 30 parts of deer's tails are added into 20 times of weights through crushing, after 200 mesh fine powder standard screens excessively respectively
The distilled water of amount mixes, and in the resulting mixture, the papain for sequentially adding mixture gross mass 0.2% is digested：
50 DEG C digest 8 hours, 100 DEG C of enzyme deactivation 5min；The compound protease for adding mixture gross mass 0.2% is digested：50℃
Enzymolysis 6 hours, 100 DEG C of enzyme deactivation 5min；The flavor protease for being eventually adding mixture gross mass 0.2% is digested：70 DEG C of enzymes
Solution 6 hours, 100 DEG C of enzyme deactivation 5min obtains complex enzyme hydrolysis liquid, is obtained after coarse filtration, the ultrafiltration membrane ultrafiltration of molecular cut off 1KDa
Small-molecular peptides concentrate is obtained, then small molecule Gly-His-Lys are obtained after nanofiltration desalination, vacuum freeze drying or spray drying.
(2) Chinese medical extract is prepared
By 5 parts of ginsengs (5 years artificial growth sun-dried ginsengs), 0.1 part of eucommia Bark male flower, 10 portions of raspberries, 20 parts of fruits of Chinese wolfberry, 10
Part jujube, 10 portions of Radix Glycyrrhizaes are uniformly mixed after crushing, and using high hydrostatic pressure extraction, extraction conditions is：The ethyl alcohol that extractant is 60%
Aqueous solution, solid-to-liquid ratio 1:5, temperature is 40 DEG C, ultra high pressure 500MPa, extracts 50min, is extracted 1 time；Second is recovered under reduced pressure
Alcohol obtains Chinese medical extract after freeze-dried.
(3) it allocates
Chinese medical extract obtained by small molecule Gly-His-Lys and step 2 obtained by step 1 is added in base liquor, per 1000mL base liquors
In, the addition of small molecule Gly-His-Lys is 0.05g, and the addition of Chinese medical extract is 10g；Add distilled water decreasing concentration to the volume of ethyl alcohol
Score is 35~42%, and white sugar 2g and honey 1g is added after mixing well, and nourishes wine after filtering up to deer ginseng small-molecular peptides.
Prepare embodiment 3
The preparation process that the deer ginseng small-molecular peptides of the present invention nourish wine is as follows：
(1) enzyme process prepares small molecule Gly-His-Lys
70 parts of deer whips, 30 parts of deer blood, 10 parts of deer's tails are added into 10 times of weights through crushing, after 300 mesh fine powder standard screens excessively respectively
The distilled water of amount mixes, and in the resulting mixture, the papain for sequentially adding mixture gross mass 0.1% is digested：
70 DEG C digest 6 hours, 100 DEG C of enzyme deactivation 5min；The compound protease for adding mixture gross mass 0.1% is digested：70℃
Enzymolysis 8 hours, 100 DEG C of enzyme deactivation 5min；The flavor protease for being eventually adding mixture gross mass 0.1% is digested：50 DEG C of enzymes
Solution 8 hours, 100 DEG C of enzyme deactivation 5min obtains complex enzyme hydrolysis liquid, is obtained after coarse filtration, the ultrafiltration membrane ultrafiltration of molecular cut off 1KDa
Small-molecular peptides concentrate is obtained, then small molecule Gly-His-Lys are obtained after nanofiltration desalination, vacuum freeze drying or spray drying.
(2) Chinese medical extract is prepared
By 1 part of ginseng (5 years artificial growth sun-dried ginsengs), 0.5 part of eucommia Bark male flower, 20 portions of raspberries, 30 parts of fruits of Chinese wolfberry, 15
Part jujube, 5 portions of Radix Glycyrrhizaes are uniformly mixed after crushing, and using high hydrostatic pressure extraction, extraction conditions is：The ethanol water that extractant is 70%
Solution, solid-to-liquid ratio 1:7, temperature is 20 DEG C, ultra high pressure 200MPa, extracts 25min, is extracted 2 times；Second is recovered under reduced pressure
Alcohol obtains Chinese medical extract after freeze-dried.
(3) it allocates
Chinese medical extract obtained by small molecule Gly-His-Lys and step 2 obtained by step 1 is added in base liquor, per 1000mL base liquors
In, the addition of small molecule Gly-His-Lys is 0.3g, and the addition of Chinese medical extract is 3g；Add distilled water decreasing concentration to the volume point of ethyl alcohol
Number is 35~42%, and white sugar 3g and honey 1g is added after mixing well, and nourishes wine after filtering up to deer ginseng small-molecular peptides.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of deer ginseng small-molecular peptides nourish wine, which is characterized in that including：
Base liquor 1000mL；
0.05~0.3g of small molecule Gly-His-Lys；
3~10g of Chinese medical extract；
0.1~0.3g of white sugar；
0.1~0.3g of honey；
The small molecule Gly-His-Lys include according to parts by weight：50~70 parts of deer whip, 10~30 parts of deer blood, 10~30 parts of deer's tail；
The Chinese medical extract includes according to parts by weight：1~5 part of ginseng, 0.1~1 part of eucommia Bark male flower, raspberry 10~30
Part, 20~40 parts of the fruit of Chinese wolfberry, 10~20 parts of jujube, 5~10 parts of Radix Glycyrrhizae.
2. preparing the method that a kind of deer ginseng small-molecular peptides described in claim 1 nourish wine, which is characterized in that including following step
Suddenly：
Step 1: enzyme process prepares small molecule Gly-His-Lys
By deer whip, deer blood, deer's tail respectively after crushing, being sieved, the distilled water mixing of 10~20 times of weight is added, is mixed in gained
Papain, compound protease, flavor protease are sequentially added in object and carries out enzyme digestion reaction, complex enzyme hydrolysis liquid are obtained, through thick
Small-molecular peptides concentrate is obtained after filtering, ultrafiltration, then obtains small molecule Gly-His-Lys after nanofiltration desalination, drying；
Step 2: preparing Chinese medical extract
It is uniformly mixed after ginseng, eucommia Bark male flower, raspberry, the fruit of Chinese wolfberry, jujube, Radix Glycyrrhizae are crushed, using high hydrostatic pressure extraction, decompression
Ethyl alcohol is recycled, Chinese medical extract is obtained after freeze-dried；
Step 3: allotment
Chinese medical extract obtained by small molecule Gly-His-Lys and step 2 obtained by step 1 is added in base liquor, adds water decreasing concentration, adds after mixing
Add white sugar and honey, nourishes wine after filtering up to deer ginseng small-molecular peptides.
3. preparation method according to claim 2, which is characterized in that further comprising the steps of before carrying out step 1：
Deer whip, deer blood, deer's tail raw material are ground into the coarse powder of 100~300 mesh after drying.
4. preparation method according to claim 2, which is characterized in that in step 1, the additive amount of the papain
It is the 0.1~0.3% of mixture gross mass；The additive amount of the compound protease is the 0.1~0.3% of mixture gross mass；
The additive amount of the flavor protease is the 0.1~0.3% of mixture gross mass.
5. preparation method according to claim 2, which is characterized in that in step 1, the papain enzymolysis reaction
Condition is：50~70 DEG C digest 6~8 hours, 100 DEG C of enzyme deactivation 5min；The compound protease reaction conditions are：50~70
DEG C enzymolysis 6~8 hours, 100 DEG C of enzyme deactivation 5min；The flavor protease reaction conditions are：50~70 DEG C of enzymolysis 6~8 are small
When, 100 DEG C of enzyme deactivation 5min.
6. preparation method according to claim 2, which is characterized in that in step 2, extraction conditions is：Solid-to-liquid ratio is 1:(5
~10), temperature is 20~40 DEG C, and ultra high pressure is 200~500MPa, and extraction time is 25~50min, extraction time 1
~2 times.
7. preparation method according to claim 2, which is characterized in that in step 2, extractant is water-soluble using ethyl alcohol
The volume fraction of liquid, ethyl alcohol is 40~70%.
8. preparation method according to claim 2, which is characterized in that in step 3, when allotment, per in 1000mL base liquors,
The addition of the small molecule Gly-His-Lys is 0.05~0.3g, and the addition of the Chinese medical extract is 3~10g, the white sugar
Addition is 0.1~0.3g, and the addition of the honey is 0.1~0.3g.
9. preparation method according to claim 2, which is characterized in that in step 3, distilled water decreasing concentration is added to ethyl alcohol
Volume fraction is 30~50%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388477A (en) * | 2021-05-26 | 2021-09-14 | 杭州医学院 | Sheep penis polypeptide wine and its production method |
| CN113462508A (en) * | 2021-06-10 | 2021-10-01 | 佳木斯大学 | Double-antler medicinal liquor and preparation method thereof |
-
2018
- 2018-04-19 CN CN201810354093.6A patent/CN108546620A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388477A (en) * | 2021-05-26 | 2021-09-14 | 杭州医学院 | Sheep penis polypeptide wine and its production method |
| CN113462508A (en) * | 2021-06-10 | 2021-10-01 | 佳木斯大学 | Double-antler medicinal liquor and preparation method thereof |
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