CN102018726A - Method for preparing animal hoof nail extract - Google Patents
Method for preparing animal hoof nail extract Download PDFInfo
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- CN102018726A CN102018726A CN2009101924311A CN200910192431A CN102018726A CN 102018726 A CN102018726 A CN 102018726A CN 2009101924311 A CN2009101924311 A CN 2009101924311A CN 200910192431 A CN200910192431 A CN 200910192431A CN 102018726 A CN102018726 A CN 102018726A
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- bauhinia variegata
- variegata linn
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- extracting solution
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Abstract
The invention discloses a method for preparing animal hoof nail extract, in particular a process for preparing the hoof nail extract by an enzymolysis method. The roof nail polypeptide extract is prepared from enzymolysis solution obtained by enzymolysis by combining a spray drying method. The roof nail extract can be prepared into various formulations which can be conveniently taken orally and applied externally by the conventional method.
Description
Technical field
The present invention relates to a kind of Bauhinia variegata Linn preparation method of extract, a kind of specifically production technology that adopts enzyme solution to prepare the animal toenail extract.
Technical background
Peptide promptly is proteinic structure fragment, is amino acid whose organic synthesis thing, and aminoacid can be called peptide with the interconnective chemical compound of amido link (also claiming peptide bond) each other, and it also is proteinic hydrolyzate, and it is the ultimate constituent of body tissue cell.Two aminoacid connect into dipeptides by peptide bond, and three aminoacid connect into tripeptides by peptide bond, and generally the peptide that 2～10 amino acid residues are formed is oligopeptide, and the peptide that a plurality of aminoacid connects into by peptide bond is a polypeptide; Polypeptide is a kind of material that is between aminoacid and the protein, and it can deeply be failed nutrient to deep skin and organize, nutrition, moisten, repair, moist, compose living cells, environment in regulating strengthens body immunity, purifies the blood blood circulation promoting.
Unguis Sus domestica is used as medicine, and is promptly on the books in Shennong's Herbal; Compendium of Material Medica records " dewclaw first "; Other all on the books as " herbal classic ", " Mingyi Bielu " etc.Among the people have a Unguis Sus domestica satin charcoal treatment function row metrorrhagia, and Ungula Sus domestica is used for the use of lactogenic.
The Bauhinia variegata Linn extract is extracting bioactive substance in pig, sheep or the Unguis Bovis seu Bubali, adopts prepared such as modern biotechnology to become the polypeptide of biologically active; The hoof nail polypeptide extract has effects such as enhancing human body immunity power.
2005 the 26th volumes of China's biochemical drug magazine the 6th phase 369-371 page or leaf, the research overview of system summary hoof nail polypeptide has especially been introduced hoof nail polypeptide and tablet development process thereof, and this medicine has been upgraded to the national standard medicine at present.
" alkalescence is extracted " method is adopted in the preparation of hoof nail polypeptide raw material at present substantially, promptly adopts alkaline matter sodium hydroxide and Bauhinia variegata Linn raw material to boil and rises, adjusts pH value, filtration, concentrated, exsiccant program preparation.The extract quality control of gained is control criterion with the content of " total nitrogen content ".This production technology is simple, but the production cost height, and standards such as quality control and remove impurity have much room for improvement.
Spray drying is that fluidization technique is used for the exsiccant a kind of method preferably of liquid material, it is the spray liquid drop that nebulizes, and dependence hot-air and droplet uniform mixing, carry out the exchange of heat exchange and matter, make the moisture rapid exsiccant process of vaporizing, obtain powdery or graininess goods, its energy consumption is lower, and can not cause that porous layer subsides; Spray drying is carried out under airtight condition of negative pressure, and the product purity height does not emit powder, is suitable for serialization large-scale production; Can regulate and control quality index such as the fineness degree of product and water content as required, small product size is little, helps the product health.In conjunction with the spraying material properties, atomisation pressure, exsiccant temperature and speed are main control parameters in the spray process, and the temperature that spray drying adopted directly influences content of peptides, immunocompetence and water content in the Bauhinia variegata Linn extract; Some bio-activity protector can form the wall film in conjunction with polypeptides matter, and high temperature resistant, hygroscopicity is little, and is tasteless, meets the polypeptide class active substance that water can discharge institute's embedding.
Summary of the invention
The object of the invention provides a kind of Bauhinia variegata Linn preparation method of extract, and it is many that this method making can be chosen raw material, is not limited only to simple Unguis Sus domestica, also can select sheep, Unguis Bovis seu Bubali for use.Preparation technology is simple, and production cost is low, and the cycle is short, and gained Bauhinia variegata Linn extract powder water content is few, and dissolubility is strong, is convenient to store and transportation, and industrial scale production is feasible.
For finishing the foregoing invention purpose, technical matters provided by the present invention is a kind ofly to prepare the Bauhinia variegata Linn extract by enzyme solution, and its special character is that its production technology is as follows:
(1) pretreatment of raw material
After the animal toenail raw material that health is fresh washed, oven dry is ground into fine grained, and was standby.
(2) preparation technology
The animal toenail granule of preparation adopts the method for enzymolysis to handle, and collects enzymolysis solution, centrifugally dispels assortedly, collects supernatant; Supernatant carries out distilling under reduced pressure and concentrates under 50-70 ℃; Concentrated solution adds the bio-activity protector spray drying, and inlet temperature 120-130 ℃, leaving air temp 50-60 ℃, be cooled to room temperature, get solid-state Bauhinia variegata Linn extract powder.
The preparation method of above-mentioned enzymolysis solution: the Bauhinia variegata Linn fine grained, add pure water in 1: 1 ratio, stir fully, add protease by gross weight 0.1-5%, heating, enzymolysis is after 0.5-1.5 hour in the time of 40-70 ℃, intensification 70-90 ℃, be incubated 3-30 minute, enzyme is lost activity.
Above-mentioned bio-activity protector adding proportion: add bio-activity protector in concentrated solution weight 0.1-3% ratio.The present invention has following advantage compared to existing technology:
1. utilize enzyme solution, can fully conveniently extract the polypeptide composition in the Bauhinia variegata Linn; Reduce the alkaline matter contact raw material or residual of traditional method.
2. when enzymolysis solution prepares the Bauhinia variegata Linn extract powder and can guarantee Bauhinia variegata Linn extract active component content of peptides in conjunction with spray drying process; Bauhinia variegata Linn extract powder yield is higher than alkali liquor extracting method commonly used at present; technology is simple; convenient for production; cycle is short; energy consumption cost is low, and can guarantee its biological activity, is fit to the scale industry and produces.
3. enzymolysis solution can make the product water content low in conjunction with utilizing spray drying process to prepare the Bauhinia variegata Linn extract powder, and the quality short texture is convenient to store and transportation.
The invention will be further elaborated below in conjunction with embodiment, but can not therefore constitute limiting the scope of the invention.
Embodiment 1:
Method of the present invention is implemented: after 100 kilograms of Unguis Sus domestica raw materials are washed, oven dry, pulverize fine grained; Fine grained adds pure water in 1: 1 ratio, stirs fully, adds protease by gross weight 0.1-5%, heating, and enzymolysis is 0.5-1.5 hour in the time of 40-70 ℃, and intensification 70-90 ℃, be incubated 3-30 minute, enzyme is lost activity.After putting cold room temperature, filter enzymolysis solution, get supernatant; Supernatant carries out distilling under reduced pressure and is concentrated into density 1.05 under 55 ℃; Concentrated solution pumps into spray drying tower after adding bio-activity protector by 1%, 130 ℃ of inlet temperature, and 60 ℃ of leaving air temps are cooled to room temperature, get solid-state Bauhinia variegata Linn extract powder, sterilization, packing, check, warehouse-in.
Simultaneously by 2005 the 26th volumes of document China biochemical drug magazine the 6th phase 369-371 page or leaf and Chinese Pharmacopoeia Commission. " alkaline extraction method " preparation sample among National Drug Administration's national drug standards chemical drugs provincial standard rising national standard (the 9th) [S] 2002.324-326 is compared as follows:
Method of the present invention and alkaline extracting method get product relatively:
Embodiment 2:
Method of the present invention is implemented: after 100 kilograms of Bauhinia purpurea raw materials are washed, oven dry, pulverize fine grained; Fine grained adds pure water in 1: 1 ratio, stirs fully, adds protease by gross weight 0.1-5%, heating, and enzymolysis is 0.5-1.5 hour in the time of 40-70 ℃, and intensification 70-90 ℃, be incubated 3-30 minute, enzyme is lost activity.After putting cold room temperature, filter enzymolysis solution, get supernatant; Supernatant carries out distilling under reduced pressure and is concentrated into density 1.05 under 55 ℃; Concentrated solution pumps into spray drying tower after adding bio-activity protector by 1%, 130 ℃ of inlet temperature, and 60 ℃ of leaving air temps are cooled to room temperature, get solid-state Bauhinia variegata Linn extract powder, sterilization, packing, check, warehouse-in.
Simultaneously by 2005 the 26th volumes of document China biochemical drug magazine the 6th phase 369-371 page or leaf and Chinese Pharmacopoeia Commission. " alkaline extraction method " preparation sample among National Drug Administration's national drug standards chemical drugs provincial standard rising national standard (the 9th) [S] 2002.324-326 is compared as follows:
Method of the present invention and alkaline extracting method get product and are compared as follows:
Embodiment 3:
With the method for the foregoing description 1 handle supernatant, prepare the Bauhinia variegata Linn extract powder directly into spray dryer, the bio-activity protector comparative result is as follows with adding:
The present invention of this presentation of results adds bio-activity protector in drying process have the extract biological action of the present invention of raising.
Embodiment 4:
Get Bauhinia variegata Linn extract powder 100 grams, prepare 1000 by following composition of raw materials by embodiment 1 or 2 preparations:
Get glucose powder 800 grams, dextrin 60 gram mixings, spray adds an amount of distilled water system soft material, 80 ℃ of oven dry, the granulate that sieves gets granule A; Get Bauhinia variegata Linn extract and magnesium stearate mixing, preparation granule B; Mixing granule A and B heavily are 1000 in 1 tablet that restrains with single punch tablet machine or rotary tablet machine tablet forming.
Embodiment 5:
Get Bauhinia variegata Linn extract powder 100 grams, restrain emollient cream by following composition of raw materials preparation 1000 by embodiment 1 or 2 preparations:
Bauhinia variegata Linn extract 100 grams
Glycerol 890 grams
Natural perfume material 10 grams
Get glycerol and be warmed up to 45 ℃, add the Bauhinia variegata Linn extract and stir fully no obvious lumps, add natural perfume material after being cooled to room temperature, make 1000 and restrain emollient cream, airtight preservation.
Claims (6)
1. Bauhinia variegata Linn preparation method of extract, it is characterized in that with the Bauhinia variegata Linn of pig, sheep or cattle clean, after the remove impurity, enzymolysis, the refining polypeptide extracting solution of being processed into, add bio-activity protector after spray drying make Bauhinia variegata Linn extract dry powder.
2. according to the described Bauhinia variegata Linn preparation method of extract of claim 1, the peptide content that it is characterized in that Bauhinia variegata Linn extract dry powder detects according to a conventional method should be greater than 10% (meter by weight).
3. according to the described Bauhinia variegata Linn preparation method of extract of claim 1, it is characterized in that the method for the refining processing and preparing polypeptide extracting solution after the homogenate is an enzymatic isolation method, comprise following processing step:
(1) animal toenail is pulverized the back fine grained and is added water in 1: 1 ratio, and the protease by adding gross weight adding 0.1～5% parts by weight behind the water stirs;
(2) be incubated enzymolysis 0.5～1.5 hour at 40～70 ℃;
(3) after enzyme digestion reaction finishes, be warming up to 70～90 ℃, be incubated 3～30 minutes;
(4) insulation finishes feed liquid is reduced to room temperature, removes solid residue in the feed liquid with centrifuging or conventional filtration method, and the supernatant liquid that obtains is the polypeptide extracting solution.
4. enzymatic isolation method according to claim 3 prepares the process of polypeptide extracting solution, it is characterized in that the protease that adds comprises animal protease, plant rennet and microbial protease.
5. according to the described Bauhinia variegata Linn preparation method of extract of claim 1, it is characterized in that the processing step that spray drying prepares Bauhinia variegata Linn extract dry powder comprises:
(1) in the polypeptide extracting solution that has prepared, add 0.1～3% bio-activity protector by weight, stir;
(2) with peristaltic pump (1) is pumped in the concentrator, 50-70 ℃ of following concentrated polypeptide extracting solution is to density range 1.01-1.15;
(3) with peristaltic pump (2) are pumped into spray drying tower, 120-130 ℃ of control inlet temperature, leaving air temp 50-60 ℃;
(4) collect spray drying institute fine powder and cross the 80-120 mesh sieve, get Bauhinia variegata Linn extract dry powder.
6. according to the purposes of the Bauhinia variegata Linn extract of the described method of claim 1 preparation, it is characterized in that to be processed into according to a conventional method and can also can be made into outer milky lotion, emollient cream, the lotion of putting use on the skin for tablet, capsule, soft capsule, pill, granule, oral liquid, Emulsion, the syrup that uses for oral administration.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101924311A CN102018726A (en) | 2009-09-18 | 2009-09-18 | Method for preparing animal hoof nail extract |
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| CN2009101924311A CN102018726A (en) | 2009-09-18 | 2009-09-18 | Method for preparing animal hoof nail extract |
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| CN102018726A true CN102018726A (en) | 2011-04-20 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104432077A (en) * | 2014-12-29 | 2015-03-25 | 唐华 | Health care preparation for preventing and treating human calcium deficiency and manufacturing method of health care preparation |
| CN104531813A (en) * | 2014-11-23 | 2015-04-22 | 内蒙古天奇生物科技有限公司 | Preparation method of cornu gorais active polypeptide |
| CN105011175A (en) * | 2015-06-09 | 2015-11-04 | 安徽汇佳生物科技有限公司 | Pig foot albumen powder and preparation method thereof |
| CN105949275A (en) * | 2016-06-21 | 2016-09-21 | 赤峰学院 | Simple method for extracting animal toenail polypeptide |
| CN111000816A (en) * | 2019-12-30 | 2020-04-14 | 重庆天致药业股份有限公司 | Hoof nail polypeptide tablet and preparation process thereof |
-
2009
- 2009-09-18 CN CN2009101924311A patent/CN102018726A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531813A (en) * | 2014-11-23 | 2015-04-22 | 内蒙古天奇生物科技有限公司 | Preparation method of cornu gorais active polypeptide |
| CN104531813B (en) * | 2014-11-23 | 2018-05-11 | 内蒙古天奇生物科技有限公司 | A kind of preparation method of cornu caprae hircus active peptides |
| CN104432077A (en) * | 2014-12-29 | 2015-03-25 | 唐华 | Health care preparation for preventing and treating human calcium deficiency and manufacturing method of health care preparation |
| CN105011175A (en) * | 2015-06-09 | 2015-11-04 | 安徽汇佳生物科技有限公司 | Pig foot albumen powder and preparation method thereof |
| CN105949275A (en) * | 2016-06-21 | 2016-09-21 | 赤峰学院 | Simple method for extracting animal toenail polypeptide |
| CN111000816A (en) * | 2019-12-30 | 2020-04-14 | 重庆天致药业股份有限公司 | Hoof nail polypeptide tablet and preparation process thereof |
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Open date: 20110420 |