CN109207550A - A kind of preparation method of peony seeds polypeptide - Google Patents
A kind of preparation method of peony seeds polypeptide Download PDFInfo
- Publication number
- CN109207550A CN109207550A CN201811398408.3A CN201811398408A CN109207550A CN 109207550 A CN109207550 A CN 109207550A CN 201811398408 A CN201811398408 A CN 201811398408A CN 109207550 A CN109207550 A CN 109207550A
- Authority
- CN
- China
- Prior art keywords
- peony seeds
- liquid
- preparation
- polypeptide
- solid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000736199 Paeonia Species 0.000 title claims abstract description 90
- 235000006484 Paeonia officinalis Nutrition 0.000 title claims abstract description 89
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 41
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 40
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 57
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 22
- 235000009566 rice Nutrition 0.000 claims abstract description 22
- 238000003756 stirring Methods 0.000 claims abstract description 19
- 238000000926 separation method Methods 0.000 claims abstract description 18
- 239000002253 acid Substances 0.000 claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 239000000843 powder Substances 0.000 claims abstract description 15
- 230000001954 sterilising effect Effects 0.000 claims abstract description 15
- 238000004090 dissolution Methods 0.000 claims abstract description 12
- 239000007791 liquid phase Substances 0.000 claims abstract description 6
- 239000007790 solid phase Substances 0.000 claims abstract description 5
- 240000007594 Oryza sativa Species 0.000 claims abstract 3
- 238000005119 centrifugation Methods 0.000 claims description 10
- 238000004108 freeze drying Methods 0.000 claims description 10
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 5
- 239000006071 cream Substances 0.000 claims description 5
- 235000013557 nattō Nutrition 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 4
- 230000009514 concussion Effects 0.000 claims description 4
- 244000057717 Streptococcus lactis Species 0.000 claims description 3
- 235000014897 Streptococcus lactis Nutrition 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 20
- 150000001413 amino acids Chemical class 0.000 abstract description 16
- 241000209094 Oryza Species 0.000 description 19
- 229940024606 amino acid Drugs 0.000 description 14
- 235000001014 amino acid Nutrition 0.000 description 14
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical group [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 239000006228 supernatant Substances 0.000 description 7
- 235000019750 Crude protein Nutrition 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 5
- 240000005001 Paeonia suffruticosa Species 0.000 description 5
- 235000003889 Paeonia suffruticosa Nutrition 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 235000015112 vegetable and seed oil Nutrition 0.000 description 4
- 230000036541 health Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 230000001376 precipitating effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 2
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 2
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 2
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 229960003767 alanine Drugs 0.000 description 2
- 235000004279 alanine Nutrition 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 229960003067 cystine Drugs 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 229960002989 glutamic acid Drugs 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 229960002449 glycine Drugs 0.000 description 2
- 239000011229 interlayer Substances 0.000 description 2
- 229960000310 isoleucine Drugs 0.000 description 2
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 229960003136 leucine Drugs 0.000 description 2
- 229930182817 methionine Natural products 0.000 description 2
- 229960004452 methionine Drugs 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229960001153 serine Drugs 0.000 description 2
- 229960004295 valine Drugs 0.000 description 2
- 239000004474 valine Substances 0.000 description 2
- VAJVDSVGBWFCLW-UHFFFAOYSA-N 3-Phenyl-1-propanol Chemical compound OCCCC1=CC=CC=C1 VAJVDSVGBWFCLW-UHFFFAOYSA-N 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- DYUQAZSOFZSPHD-UHFFFAOYSA-N Phenylpropyl alcohol Natural products CCC(O)C1=CC=CC=C1 DYUQAZSOFZSPHD-UHFFFAOYSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- 239000012445 acidic reagent Substances 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- 229960003646 lysine Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 229960005190 phenylalanine Drugs 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012414 sterilization procedure Methods 0.000 description 1
- 229960002898 threonine Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention provides a kind of preparation methods of peony seeds polypeptide, belong to polypeptide preparation technical field, the described method comprises the following steps: 1) by after peony seeds dregs of rice powder and water mixed dissolution, adjusting pH value is 8~10, and 40~80min of stirring obtains alkaline feed liquid；2) the alkaline feed liquid obtained in step 1) is separated by solid-liquid separation and obtains alkaline clear liquid, the pH value for adjusting the alkaline clear liquid is 2~4, stirs 15~25min, obtains acid feed liquid；3) after being separated by solid-liquid separation the acid feed liquid, solid phase components is collected and obtain peony seeds albumen；4) after the peony seeds albumen being mixed to sterilizing with water, 20~40 DEG C of 60~80h of fermentation of strain are accessed, are separated by solid-liquid separation, collection liquid phase component is peony seeds polypeptide.Peony seeds polypeptide good water solubility (solubility 50mg/mL) that the method for the invention prepares, amino acid content are more than or equal to 10%, and the type of contained common amino acid is at 15 kinds or more.
Description
Technical field
The invention belongs to polypeptide preparation technical field more particularly to a kind of preparation methods of peony seeds polypeptide.
Background technique
Tree peony belongs to Paeoniaceae Paeonia, is perennial deciduous shrub, be a kind of economic value and nutritive value all very
High plant.Peony seeds can be used for extracting oil, and seed oil is rich in alpha-linolenic acid, have the plurality of health care functions such as lowering blood pressure and blood fat；
The root bark of tree peony rich in moutan root bark, can be used for producing Chinese medicine；Flower, flower bud, the bud of tree peony can be used for making health protection tea；Peony seeds
Grouts are rich in dietary fiber, can be used for fermenting and producing ferment, adjust gastrointestinal health.
Peony seed oil sharply expands as new resource food, cultivated area at present, and peony seeds yield improves year by year, to tree peony
Research it is also more and more, more and more deep.The peony seeds grouts that grease generates after extracting are mainly as feedstuff or eat
Bacterium culture medium base-material, comprehensive development and utilization level are low；Peony seeds cake protein content is about 30%, currently, to peony seeds albumen
The research extracted and utilized also lacks very much, still without a kind of effective method extracted and utilize peony seeds albumen.
Summary of the invention
In view of this, the contents of the present invention are to provide a kind of preparation method of peony seeds polypeptide, the method utilizes alkali
The molten heavy method of acid extracts peony seeds albumen, then accesses strain and ferments to the peony seeds albumen, peony seeds albumen is converted
For polypeptide form, it is more advantageous to the absorption of human body, reaches health-care effect, improves the nutritive value of the peony seeds dregs of rice.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical schemes:
A kind of preparation method of peony seeds polypeptide, comprising the following steps:
1) by after peony seeds dregs of rice powder and water mixed dissolution, adjusting pH value is 8~10, and 40~80min of stirring obtains alkalinity material
Liquid；
2) the alkaline feed liquid obtained in step 1) is separated by solid-liquid separation and obtains alkaline clear liquid, adjust the pH value of the alkaline clear liquid
It is 2~4, stirs 15~25min, obtains acid feed liquid；
3) after being separated by solid-liquid separation the acid feed liquid, solid phase components is collected and obtain peony seeds albumen；
4) after the peony seeds albumen being mixed to sterilizing with water, 20~40 DEG C of 60~80h of fermentation of strain, solid-liquid point are accessed
From collection liquid phase component is peony seeds polypeptide.
Preferably, the mass ratio of the powder of the peony seeds dregs of rice described in step 1) and water is 1:(10~40).
Preferably, the temperature of mixed dissolution described in step 1) is 45~65 DEG C.
Preferably, separation of solid and liquid described in step 2), step 3) and step 4) is centrifugation.
Preferably, the revolving speed of stirring described in step 1) is 50~200rpm.
Preferably, the mass ratio of peony seeds albumen described in step 4) and water is 1:2~8.
Preferably, strain described in step 4) is Lactococcus lactis cream subspecies, streptococcus thermophilus, bafillus natto and wine
One of brewer yeast.
Preferably, with concussion during fermenting described in step 4), the revolving speed of the concussion is 30~100rpm.
Preferably, step 4) further includes that the peony seeds polypeptide is dried to obtain after obtaining the peony seeds polypeptide
Obtain peony seeds polypeptide powder.
Preferably, the drying is freeze-drying, and the temperature of the freeze-drying is -80~-60 DEG C；The freeze-drying
Time be 75~85h.
Beneficial effects of the present invention: the preparation method of peony seeds polypeptide provided by the invention, first with alkali extraction-acid precipitation
The peony seeds albumen in the peony seeds dregs of rice is extracted, strain is then accessed and ferments to the peony seeds albumen, by peony seeds albumen
It is converted into polypeptide form, is more advantageous to the absorption of human body, it is without any side effects, reach health-care effect, improves the peony seeds dregs of rice
Nutritive value.Peony seeds polypeptide good water solubility (solubility 50mg/mL), the cost that the method for the invention prepares
It is low, amino acid content is high, type is abundant, and the type of contained common amino acid is at 15 kinds or more.
For the residue for extracting peony seed oil, utilization is carried out to it to be had the peony seeds dregs of rice that institute's book method of the present invention uses
Effect improves productional surplus price value；The method of the invention is easy to operate, it is low in cost, be easy to industrialization production；Institute of the present invention
It states method and provides technical foundation for the exploitation of the national peony seeds dregs of rice and higher value application.
Specific embodiment
The present invention provides a kind of preparation methods of peony seeds polypeptide, comprising the following steps: 1) by peony seeds dregs of rice powder and water
After mixed dissolution, adjusting pH value is 8~10, and 40~80min of stirring obtains alkaline feed liquid；2) alkalinity obtained in step 1) is expected
Liquid, which is separated by solid-liquid separation, obtains alkaline clear liquid, and the pH value for adjusting the alkaline clear liquid is 2~4, stirs 15~25min, obtains acid material
Liquid；3) after being separated by solid-liquid separation the acid feed liquid, solid phase components is collected and obtain peony seeds albumen；4) by the peony seeds albumen with
After water mixing sterilizing, 20~40 DEG C of 60~80h of fermentation of strain are accessed, are separated by solid-liquid separation, collection liquid phase component is peony seeds polypeptide.
The present invention is by peony seeds dregs of rice powder and water mixed dissolution.The granularity of the peony seeds dregs of rice powder is preferably in the present invention
0.5~2mm, more preferably 1~1.5mm.Heretofore described peony seeds dregs of rice powder is to be crushed to obtain by the peony seeds dregs of rice, the present invention couple
The method of the crushing is not particularly limited, if can be realized crush after peony seeds dregs of rice powder granularity within above range i.e.
It can；In specific implementation process of the present invention, described crush is carried out using pulverizer.In the present invention, the peony seeds dregs of rice are tree peony
Seed extracts the residue after peony seed oil, from a wealth of sources, at low cost.The present invention by the peony seeds dregs of rice powder and water mixed dissolution,
The mass ratio of the peony seeds dregs of rice powder and water is preferably 1:(10~40), more preferably 1:(15~35), most preferably 1:(20~
30)；The temperature of heretofore described mixed dissolution is preferably 45~65 DEG C, more preferably 50~60 DEG C, most preferably 52~58
℃；The time of heretofore described mixed dissolution is preferably 3~8min, more preferably 5min；Heretofore described mixed dissolution
During it is preferred with stirring, the revolving speed of the stirring is preferably 80~120rpm, more preferably 100rpm.
For the present invention after the mixed dissolution, adjusting pH value is 8~10, and 40~80min of stirring obtains alkaline feed liquid.At this
It is preferably alkaline reagent that the reagent of pH value is adjusted described in invention, more preferably highly basic, in specific implementation process of the present invention,
The reagent for adjusting pH value is NaOH solution, and the concentration of the NaOH solution is preferably 0.5~1.5mol/L, more preferably
1.0mol/L.In the present invention, the pH value is preferably 8.5~9.5.In the present invention, the time of the stirring is preferably 50~
70min, more preferably 55~65min.
For the present invention after obtaining the alkaline feed liquid, the alkaline feed liquid of acquisition, which is separated by solid-liquid separation, obtains alkaline clear liquid, adjusts institute
The pH value for stating alkaline clear liquid is 2~4, stirs 15~25min, obtains acid feed liquid.In the present invention, the separation of solid and liquid is preferably
Centrifugation, the revolving speed of heretofore described centrifugation is preferably 3000~8000rpm, more preferably 4000~7000rpm；The centrifugation
Time be preferably 10~40min, more preferably 15~35min；The heretofore described supernatant that is collected after centrifugation is alkaline clear
Liquid.The reagent that the pH value of the alkaline clear liquid is adjusted in the present invention is preferably acid reagent, more preferably hydrochloric acid solution, the salt
The concentration of acid solution is preferably 0.5~1.5mol/L, more preferably 0.8~1.2mol/L, and in the present invention, the pH value is preferred
Be adjusted to 2.5~3.5, be more preferably adjusted to 2.8~3.3.The present invention is after the pH value for adjusting the alkaline clear liquid, stirring 15
~25min obtains acid feed liquid；The time of the stirring is preferably 18~22min, more preferably 20min.
The acid feed liquid is separated by solid-liquid separation by the present invention after obtaining the acid feed liquid, and it is male to collect solid phase components acquisition
Red seed albumen.In the present invention, the separation of solid and liquid is preferably centrifuged, and the revolving speed of heretofore described centrifugation is preferably 3000~
8000rpm, more preferably 4000~7000rpm；The time of the centrifugation is preferably 10~40min, more preferably 15~
35min。
The peony seeds albumen will be mixed sterilizing with water after obtaining the peony seeds albumen by the present invention.In the present invention
In, the mass ratio of the peony seeds albumen and water is preferably 1:2~8, more preferably 1:(3~7) it is most preferably 1:4.The present invention
The method of the sterilizing is not particularly limited, using this field conventional sterilization procedures.In specific implementation process of the present invention
In, using high-temperature heat sterilization, ultraviolet sterilization and film filtration sterilization.The present invention after the sterilizing, access strain 20~
40 DEG C of 60~80h of fermentation are separated by solid-liquid separation, and collection liquid phase component is peony seeds polypeptide.In the present invention, the strain is lactic acid cream
One of coccus cream subspecies, streptococcus thermophilus, bafillus natto and saccharomyces cerevisiae, the present invention does not have the source of the strain
There is particular determination, using the conventional above-mentioned strain in this field, in specific implementation process of the present invention, the strain is purchased from Beijing
The big Bioisystech Co., Ltd of hundred Ou Bo.In the present invention, the temperature of the fermentation is depending on the optimum temperature of specific strain, choosing
It is 25~35 DEG C, 28~32 DEG C or 30 DEG C.The time of heretofore described fermentation is preferably 65~75h, more preferably 72h.This
Strain described in invention is the strain after activation, and the present invention is not particularly limited the activation method of the strain, using routine
Actication of culture method.In the present invention, the mass ratio of the quality that accesses strain and peony seeds albumen be preferably 1:(2000~
3000), more preferably 1:(2200~2800), most preferably 1:2500.The present invention it is described after fermentation, carry out solid-liquid point
From collection liquid phase component is peony seeds polypeptide.In the present invention, the separation of solid and liquid is preferably centrifuged, heretofore described centrifugation
Revolving speed be preferably 3000~8000rpm, more preferably 4000~7000rpm；The time of the centrifugation is preferably 10~
40min, more preferably 15~35min.The present invention after obtaining the peony seeds polypeptide, further include to the peony seeds polypeptide into
Row is dry to obtain peony seeds polypeptide powder, and in the present invention, the drying is preferably freeze-dried, and the temperature of the freeze-drying is excellent
It is selected as -80~-60 DEG C, more preferably -75~-65 DEG C, most preferably -70 DEG C；The time of the freeze-drying is preferably 75~
85h, more preferably 78~82h, most preferably 70h.
The peony seeds polypeptide that the method for the invention prepares, good water solubility (solubility 50mg/mL), it is at low cost,
Amino acid content is high, is more than or equal to that 10%, amino acid contained type is abundant, the type of contained common amino acid 15 kinds with
On, it is more advantageous to the absorption of human body.
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
Peony seeds dregs of rice 200g is weighed, is crushed (0.5~2mm of granularity) with pulverizer, is then added in 5000g water 53 DEG C
Water bath with thermostatic control stirring, mixing time 5min, speed of agitator 100rpm, then pH to 9.25 is adjusted with 1mol/LNaOH solution,
68min is stirred, it is rear to be centrifuged (5000rpm, 20min), pH to 3.5 is adjusted 1mol/L HCl solution is added to its supernatant, is stirred
It is centrifuged (5000rpm, 20min) after mixing 20min, obtaining precipitating is crude protein 50g, and the mixing of 200g water is added to the crude protein
It uniformly sterilizes spare.Bafillus natto is activated into 72h in 30 DEG C of incubator.The thick egg that above-mentioned sterilizing is dissolved in water again
The bacillus natto to ferment 72h that the white 20mg of access in superclean bench has been activated, fermentation temperature is 30 DEG C, and shaking table speed is
50rpm.It is centrifuged (5000rpm, 20min) collection supernatant after fermentation to be lyophilized, freeze temperature is -70 DEG C, time 80h.
Finally obtain 38g peony seeds polypeptide.
Obtained peony seeds polypeptide good water solubility (solubility 50mg/mL), at low cost, total amino acid content are
12.36%, 20 kinds of common amino acid types are not less than 15 kinds.It include: asparatate, Soviet Union in the peony seeds polypeptide obtained
Propylhomoserin, serine, glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, phenylpropyl alcohol ammonia
Acid, lysine, arginine and proline.
Embodiment 2
Peony seeds dregs of rice 10kg is weighed, is crushed (granularity 1mm) with pulverizer, 53 DEG C of constant temperature in 250kg water are then added
In water-bath interlayer agitator tank, mixing time 5min, speed of agitator 100rpm, then adjust pH with 1mol/L NaOH solution and arrive
9.25,68min is stirred, it is rear to be centrifuged (5000rpm, 20min), 1mol/L HCl solution is added to its supernatant and adjusts pH to 3.5,
It is centrifuged (5000rpm, 20min) after stirring 20min, obtaining precipitating is crude protein 2.5kg, and 10kg water is added and is uniformly mixed sterilizing
It is spare.Bafillus natto is activated into 72h in 30 DEG C of incubator.In the crude protein that above-mentioned sterilizing is dissolved in water ultra-clean
The bacillus natto to ferment 72h that 1g has been activated is accessed in workbench, fermentation temperature is 30 DEG C, revolving speed 50rpm.After fermentation from
The freeze-drying of the heart (5000rpm, 20min) supernatant, freeze temperature are -70 DEG C, time 80h.It is more to finally obtain 1.9kg peony seeds
Peptide.Obtained peony seeds polypeptide good water solubility (solubility 50g/mL), at low cost, total amino acid content 11.71%, 20 kind
Common amino acid type is not less than 15 kinds, include: in the peony seeds polypeptide of acquisition asparatate, threonine, serine,
Glutamic acid, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, phenylalanine, lysine, essence
Propylhomoserin, proline).
Embodiment 3
Peony seeds dregs of rice 500g is weighed, is crushed (granularity 1mm) with pulverizer, 53 DEG C of constant temperature in 12500g water are then added
In water-bath interlayer agitator tank, mixing time 5min, speed of agitator 100rpm, then adjust pH with 1mol/LNaOH solution and arrive
9.25,68min is stirred, it is rear to be centrifuged (5000rpm, 20min), 1mol/L HCl solution is added to its supernatant and adjusts pH to 3.5,
It is centrifuged (5000rpm, 20min) after stirring 20min, obtaining precipitating is crude protein 120kg, and 480g water is added and is uniformly mixed sterilizing
It is spare.Saccharomyces cerevisiae is activated into 72h in 30 DEG C of incubator.In the crude protein that above-mentioned sterilizing is dissolved in water in ultra-clean work
The fermentation by saccharomyces cerevisiae 72h that 0.05g has been activated is accessed in platform, fermentation temperature is 30 DEG C, revolving speed 50rpm.It is centrifuged after fermentation
The freeze-drying of (5000rpm, 20min) supernatant, freeze temperature are -70 DEG C, time 80h.Finally obtain 80g peony seeds polypeptide.Ammonia
Base acid total content is 5.63%.By the same method as above: used streptococcus thermophilus ferments, and finally obtains 71g peony seeds
Polypeptide, total amino acid content 7.39%.Used Lactococcus lactis cream subspecies are fermented, and 74g peony seeds polypeptide is finally obtained,
Total amino acid content is 7.86%.
As can be seen from the above embodiments, the peony seeds polypeptide that the method for the invention prepares, (solubility is good water solubility
50mg/mL), at low cost, amino acid content is high, be more than or equal to that 10%, amino acid contained type is abundant, contained common amino acid
Type at 15 kinds or more, be more advantageous to the absorption of human body.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of preparation method of peony seeds polypeptide, comprising the following steps:
1) by after peony seeds dregs of rice powder and water mixed dissolution, adjusting pH value is 8~10, and 40~80min of stirring obtains alkaline feed liquid；
2) the alkaline feed liquid obtained in step 1) is separated by solid-liquid separation and obtains alkaline clear liquid, the pH value for adjusting the alkaline clear liquid is 2
~4,15~25min is stirred, acid feed liquid is obtained；
3) after being separated by solid-liquid separation the acid feed liquid, solid phase components is collected and obtain peony seeds albumen；
4) after the peony seeds albumen being mixed to sterilizing with water, 20~40 DEG C of 60~80h of fermentation of strain are accessed, are separated by solid-liquid separation, are received
Integrate liquid phase component as peony seeds polypeptide.
2. preparation method according to claim 1, which is characterized in that the quality of the powder of the peony seeds dregs of rice described in step 1) and water
Than for 1:(10~40).
3. preparation method according to claim 1 or 2, which is characterized in that the temperature of mixed dissolution described in step 1) is
45~65 DEG C.
4. preparation method according to claim 1, which is characterized in that solid-liquid described in step 2), step 3) and step 4)
It is separated into centrifugation.
5. preparation method according to claim 1 or 2, which is characterized in that the revolving speed of stirring described in step 1) be 50~
200rpm。
6. preparation method according to claim 1, which is characterized in that the quality of peony seeds albumen described in step 4) and water
Than for 1:2~8.
7. preparation method according to claim 1, which is characterized in that strain described in step 4) is that Lactococcus lactis cream is sub-
One of kind, streptococcus thermophilus, bafillus natto and saccharomyces cerevisiae.
8. preparation method according to claim 1 or claim 7, which is characterized in that adjoint during fermenting described in step 4)
Concussion, the revolving speed of the concussion are 30~100rpm.
9. preparation method according to claim 1, which is characterized in that step 4) is after obtaining the peony seeds polypeptide, also
Peony seeds polypeptide powder is obtained including the peony seeds polypeptide is dried.
10. preparation method according to claim 9, which is characterized in that the drying is freeze-drying, the freeze-drying
Temperature be -80~-60 DEG C；The time of the freeze-drying is 75~85h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811398408.3A CN109207550A (en) | 2018-11-22 | 2018-11-22 | A kind of preparation method of peony seeds polypeptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811398408.3A CN109207550A (en) | 2018-11-22 | 2018-11-22 | A kind of preparation method of peony seeds polypeptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109207550A true CN109207550A (en) | 2019-01-15 |
Family
ID=64994180
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811398408.3A Pending CN109207550A (en) | 2018-11-22 | 2018-11-22 | A kind of preparation method of peony seeds polypeptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109207550A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114767566A (en) * | 2022-04-12 | 2022-07-22 | 广州优理氏生物科技有限公司 | Peony seed protein powder, preparation method, yeast repair essence cream and application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130105003A (en) * | 2012-03-16 | 2013-09-25 | 코스맥스 주식회사 | Cosmetic composition for improving atopic skin comprising herb extract fermented in bamboo cylinder and manufacturing method thereof |
| CN103937865A (en) * | 2014-04-28 | 2014-07-23 | 李�杰 | Peony polypeptide as well as preparation method and application thereof |
| CN106281659A (en) * | 2016-08-30 | 2017-01-04 | 铜陵亚通牡丹产业发展有限公司 | Method for extracting protein in a kind of seed of Flos Moutan dregs of rice |
| CN107523599A (en) * | 2017-09-01 | 2017-12-29 | 兰溪市沉默生物科技有限公司 | The method that fermentation method prepares peony pollen polypeptide |
| CN108823273A (en) * | 2018-07-09 | 2018-11-16 | 齐鲁工业大学 | A kind of peony seeds dregs of rice polypeptide and its preparation method and application with antioxidant activity |
-
2018
- 2018-11-22 CN CN201811398408.3A patent/CN109207550A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130105003A (en) * | 2012-03-16 | 2013-09-25 | 코스맥스 주식회사 | Cosmetic composition for improving atopic skin comprising herb extract fermented in bamboo cylinder and manufacturing method thereof |
| CN103937865A (en) * | 2014-04-28 | 2014-07-23 | 李�杰 | Peony polypeptide as well as preparation method and application thereof |
| CN106281659A (en) * | 2016-08-30 | 2017-01-04 | 铜陵亚通牡丹产业发展有限公司 | Method for extracting protein in a kind of seed of Flos Moutan dregs of rice |
| CN107523599A (en) * | 2017-09-01 | 2017-12-29 | 兰溪市沉默生物科技有限公司 | The method that fermentation method prepares peony pollen polypeptide |
| CN108823273A (en) * | 2018-07-09 | 2018-11-16 | 齐鲁工业大学 | A kind of peony seeds dregs of rice polypeptide and its preparation method and application with antioxidant activity |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114767566A (en) * | 2022-04-12 | 2022-07-22 | 广州优理氏生物科技有限公司 | Peony seed protein powder, preparation method, yeast repair essence cream and application |
| CN114767566B (en) * | 2022-04-12 | 2022-11-29 | 广州优理氏生物科技有限公司 | Peony seed protein powder, preparation method, yeast repair essence cream and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103815141B (en) | Folium Eucommiae fodder additives and its preparation method | |
| CN101731444B (en) | Pea peptide nutriment and preparation method thereof | |
| CN104187947A (en) | Hericium erinaceus liquid fermented beverage and preparation method thereof | |
| CN102660436A (en) | Oyster yellow wine | |
| CN103907987A (en) | Method for preparing hawthorn fruit vinegar beverage by continuously fermenting various mixed strains | |
| CN101914472A (en) | Eucommia leaf extract and preparation method and application thereof | |
| CN111657053A (en) | Simulated wild high-quality high-yield standardized cultivation method for cordyceps militaris | |
| CN104726285A (en) | Proanthocyanidins-rich health liquor and making method thereof | |
| CN102351580A (en) | Method for preparing black fungus nutrient solution by utilizing food processing residues | |
| CN104388507B (en) | A kind of strange sub- seed polypeptide and preparation method thereof | |
| CN110616122A (en) | Cardamine hupingshanensis selenium-rich yellow wine and production method thereof | |
| CN102907568B (en) | Cold-region fermented soybean meal industrialized production method | |
| CN101999673B (en) | Process for extracting dietary fibers from peony leaves by fermentation method | |
| CN109006179A (en) | A kind of production method of mushroom cultivation substrate | |
| CN103783263A (en) | Folium cortex eucommiae and sweet potato mixed fermentation product and preparation method thereof | |
| CN109207550A (en) | A kind of preparation method of peony seeds polypeptide | |
| CN109182405A (en) | A method of γ-polyglutamic acid is produced using lichens bud pole bacterium solid state fermentation | |
| CN102860412A (en) | Co-production method for distilled spirit and livestock total nutrient feed through alfalfa fermentation | |
| CN102018726A (en) | Method for preparing animal hoof nail extract | |
| CN105111003A (en) | Liquid organic fertilizer and preparing method thereof | |
| CN107568159A (en) | A kind of cultural method for promoting Shelled Turtle Trionyx Sinensis growth | |
| CN104402618A (en) | Pleurotus cornucopiae cultivation material utilizing garlic straws and method for producing pleurotus cornucopiae by utilizing garlic straws as cultivation materials | |
| CN1586300A (en) | Method for preparing health food for improving immunological function | |
| CN111587976B (en) | Method for extracting bioactive functional components from selenium-rich tea and application thereof | |
| CN110195086A (en) | A method of gamma-polyglutamic acid is produced using soy sauce residues solid state fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |