KR20130105003A - Cosmetic composition for improving atopic skin comprising herb extract fermented in bamboo cylinder and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving atopic dermatitis comprising as an active ingredient an extract of Zygomycin, Baekjiae, Baekji, Astragalus, Leguminosae, Lycopersicum, Compositions and methods for their preparation.
The present invention relates to a herbal composition comprising 5 to 22% by weight of a raw material for herbal medicine, 1 to 15% by weight of white paper, 1 to 15% by weight of herbaceous plant, 1 to 15% by weight of Puerariae, 5 to 22% By weight, from 5 to 40% by weight, from 1 to 15% by weight, from 1 to 15% by weight, from 1 to 15% by weight and from 5 to 40% by weight, After filtration through a filter paper, the concentrate was concentrated under reduced pressure, and the concentrate was sterilized at a high temperature in a bamboo container. Then, yeast ( Saccharomyces cerevisiae ) is added and fermented at 20 to 30 ° C for a predetermined period (1 to 7 days) to produce a fermented bamboo vinegar extract.
The present invention relates to a cosmetic (skin lotion), a nutrient lotion, a nutritional cream, a massage cream, a nutritional essence, a cleanser, a lotion or the like in an amount of 0.001% to 60%, preferably 1.0% And can be added to various cosmetics such as pharmaceutical formulations.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for improving atopic skin comprising a fermented extract of a bamboo plant herbarium as an active ingredient and a method for producing the same.

The present invention relates to a method for improving the effect of moisturizing and atopic skin, which comprises extracting a herbal medicine raw material composed of Zyseil, Baekji, Astragalus, Puerariae, Licorice, Licorice, Peony, The present invention relates to a cosmetic composition for improving atopic skin and having excellent effects in moisturizing effect, anti-inflammatory effect and free radical scavenging effect, which comprises a fermented bamboo oil fermented extract obtained by fermentation and aging for a long period of time using a bamboo barrel .

In general, atopy is a Greek word that means 'abnormal response', 'weird', or 'unknown', and as it is, atopic dermatitis is complicated by various causes and causes remission and recurrence. It is a difficult skin disease.

Atopy refers to a series of allergic symptoms that appear in the skin, respiratory mucosa, eye mucosa, and intestinal mucosa in individuals with atopic predisposition. These atopic predispositions (allergic constitutions) are inherited and appear to be family. , Allergic rhinitis, asthma, allergic conjunctivitis, atopic urticaria and the like, these diseases may be present alone or several diseases at the same time.

The mechanism of atopic allergy is that when allergens invade through the stratum corneum and reach the epidermal cells, macrophages (macrophages), which are a type of leukocytes, digest them and break them down. If the macrophage is unable to cope with a large amount of invaded allergen, information on the presence of allergens that have not yet been processed is sent to the same white blood cell T cell (responsible for the regulation of immune stimulation and inhibition) , T cells transmit the information to the eosinophil (eosinophilic leukocyte), a kind of white blood cell, and collect eosinophils, which are widely distributed in the blood, into the place where the allergen has invaded.

At this time, eosinophils accumulated in the lesion cause inflammation due to excessive release of the chemical transfer substance (active oxygen), which is the cause of the allergic reaction.

Symptoms of atopic dermatitis (severe fever) have severe itching. In acute phase, the skin becomes itchy, reddened, swollen, bloomed, blistered, blistered, damp, and itchy in the chronic phase and hard or thick Or scales such as rice flour are sitting, blackened, or whitened.

Atopic dermatitis usually begins with an infantile eczema, often called a fever.

Steroids (corticosteroids) among the currently used medicines have largely anti-inflammatory and immunosuppressive properties and are excellent in their effectiveness.

A caveat is the side effects of steroids.

If applied for a long time, hair on the right area may become hairy, the skin may be shrunk, the skin color becomes less and the bacteria become infected, the acne grows, the skin becomes thinner,

In severe cases, hormonal systemic symptoms may occur.

In addition, stopping the drug causes the symptoms to explode again, making it impossible to stop the drug.

Systemic administration of the adrenal cortex is not recommended because the age of onset of atopic dermatitis is common in infants before the age of five.

Therefore, when a steroid ointment with a very low hormone concentration is only needed for a short period of time, it should be applied in small quantities rather than in large quantities at once.

Of course, you should not use it arbitrarily, but you must be prescribed by a specialist.

Antihistamines help prevent the release of histamine in mast cells, thus alleviating the itchy symptoms, but they are used as a temporary measure. There are side effects such as burning, anxiety and loss of appetite when taken over a long period of time.

Atopic dermatitis is a more frightening disease with secondary bacterial infections that are more itchy than scratching itself.

Antibiotics are essential for the treatment of atopy.

The skin of atopic patients is accompanied by bacterial infection as a result of prolonged scraping and drying.

More than 90% of atopic patients are infected with Staphylococcus aureus, which is caused by scratching the patient's irritated itch, but recent reports suggest that the bacteria 's exotoxin stimulates the body' s immune system and causes allergic chemicals And atopic dermatitis.

In other words, the bacteria themselves act as allergens.

Therefore, the use of appropriate antibiotics is essential for the treatment of atopy.

Other nonsteroidal ointment is a kind of moisturizer. It is good to continue use even after getting good skin.

In addition, sedatives and calming agents are used to soothe and at night, ointments are applied for moisturizing, followed by wrapping with a lap.

In recent years, research on natural product preparations is underway.

(KISTI report 2003, Sohn Eun Soo) It is known that a preparation made from a natural product has a low side effect, its action is generally mild and persistent, and an insoluble component as an auxiliary component is used as a usable component. .

As an example of such a natural product preparation, Korean Patent Registration No. 0431076 discloses a cosmetic composition for improving healing of atopic skin comprising a white lotus, a potato, an echinacea and a fermented soybean extract. In Korean Patent Application Publication No. 2004-0065126, And herbal botanical extracts obtained from Ganoderma Lucidum, Ganoderma Lucidum, Lycopersicon esculentum, Lycopersicon esculentum, Bacillus sp.

In Korea registered patent No. 1081913, a herbal medicine extract composed of Gakyung, Dum, Yeonjong, Gijja, Huangbai, Golden, Huangli, Gianfu, Peony, And the resulting filtrate is used as an effective ingredient for fermentation which has an excellent effect on skin improvement such as moisturizing, skin irritation alleviation, atopic improvement, anti-inflammatory effect, etc. An extracellular solvent composition containing herbal extracts is disclosed.

In the above-mentioned patent, there is a problem in that a fermented herbal extract must be prepared by a complicated process in which a mushroom is cultured and cultured in a synthetic medium in which a herbal extract and a sugar are mixed, and there is a fear of secondary contamination such as heavy metals there was.

However, a natural preparation satisfactory for improving atopic skin diseases without side effects has not yet been developed, while still providing moisturizing effect, anti-inflammation, free radical scavenging effect and the like, which are indispensable components of the atopic treatment agent.

Therefore, it is necessary to develop a substance that satisfies the moisturizing effect, anti-inflammatory effect, free radical scavenging effect, and the like, which are essential ingredients of the atopic treatment agent and has no side effects.

In response to the above-mentioned demand of the present invention, it has been found that a substance having excellent efficacy such as excellent moisturizing effect, anti-inflammatory effect, free radical scavenging effect and safety is extracted from a natural product for improvement of atopic skin, The present invention provides a fermented extract obtained by the above method.

Another object of the present invention is to provide a cosmetic composition comprising a fermented extract having such an effect.

In order to accomplish the above object, the present invention provides a method for treating atopic diseases, comprising the steps of: (a) It is very effective in moisturizing effect, anti-inflammatory effect, free radical scavenging effect by fermenting microbes in a bamboo tube after fermentation. And bamboo fruit juice extracts were used as the herb extracts of Bacillus spp., Bacillus sp., Bacillus thuringiensis, Puerariae radix, Bacillus thuringiensis, Licorice, By using this, it is possible to solve the safety problem at the same time by generating a natural fermentation extract without contamination.

In addition, it was confirmed that the skin moisturizing and soothing effect is exhibited even when the above ingredients are mixed with the cosmetic composition at a certain concentration or more as an active ingredient and applied directly to human skin.

Hereinafter, the materials used in the present invention will be described.

Poncirus trifoliata Rafinesqul is a dried young fruit of the tangerine tree. It contains flavonoid glycosides, vitamin C, and acetic acid. It eliminates the debris, removes gas, heals the gastrointestinal tract and anal escape , Is effective in treating uterine escape.

In addition, it has been effective in the treatment of dermatosis, pharmacological studies have revealed that there are contractions of the uterus, gastrointestinal motility, hyperactivity, arteriosclerosis and diuretic effect.

It also contains ingredients such as tangeretin, nobiletin, cinephrine, and hesperidin, which inhibit histamine release, inhibit appetite and inhibit cholesterol.

Angelica Dahuricae Radix is a perennial herb that grows in grassy bushes, bushes or riversides, and is harvested in the sun and dried in the sun.

According to conventional identification studies, materials such as Cumarins, Byak-angelicin, Oscine, and Xangthotoxin are known.

White paper is traditionally known to treat fever with heat or wind, and it is also made by taking pills to make things worse by the wind.

In one room, it is used as analgesic, hemostasis, and sedative.

Studies related to white paper have shown that treatment of dermatitis by promotion of sebum secretion (Japanese Patent Laid-Open No. 9-176030), hair growth inhibitor having excellent human stability (Japanese Patent Laid-Open Publication No. 10-139639) And it is said that the above blank paper is made into the plaster and the minus in the Dongbuibogam and the sacred Maternity manure, and when it is applied to the face, the warm aura is sent to the face skin to promote the circulation of the blood to make the complexion fertile, and to remove the spots, freckles and scars on the face .

Cnidium officinale Makino is a perennial herb that is native to China and cultivated as a medicinal crop. Its height is 30 to 60 cm and its components are cnidilide, ligustilide, It is known to contain neocnidilide, butylphthalide, and sedanonic acid, and it has a characteristic of smelling plants.

The root of the cystic mantle is an irregular nodule mass with a diameter of 2 to 7 cm. The surface is yellowish brown with several ridges rising in parallel with rough wrinkles, with concave circular stem marks on the top, It is yellow but yellowish brown is scattered. Formation layer is wavy.

The rootstock was dried and used in oriental medicine, and it was used for gynecological diseases such as uterus sedation, blood pressure control, antimicrobial action, inhibition against dermatophytes, headache, anemia, infertility, menstrual irregularity and poor circulation. I put them in the wardrobe for some prevention in the private sector (Ibsub, Shin Min-gyo, Ambassador's Dictionary).

Pueraria lobata Ohwi is a dried perennial herbaceous plant root of the legume plant, and it grows in mountains of Korea, China and Japan.

In Chinese medicine, the various parts of the 칡 are classified, and the dried root of 칡 is called root, the flower is brown, the fruit is brown or brown, the leaves are brown, and the vine is brown, and it has been used differently depending on the symptoms and treatment of the disease. .

It is used for relieving cold body aches, digestive diseases, and intestinal tract. It helps the spleen and stomach to rise, so it can help to relieve thirst and make a branch. do.

The major components are deoxymyestrol (deoxymyestrol) and puerarin, which are isoflavonoids.

Antidipsotropic, hepatoprotective, hangover inhibitory, and antioxidant activities are known as pharmacological actions related to Puerariae.

Forsythia koreana Nakai is a fruit of the forsythia belonging to the Oleaceae family.

The forsythia is a deciduous shrub with a height of 2 ~ 3m and its branches are thin and long, spread or fallen downward and hollow. Flowers bloom earlier than leaves, and 1 to 4 flowers blossom on leaves.

The fruit is a narrow oval shape with a short beak at the end. When fully ripe, it is split into two pieces. It grows in the wilderness of Sanya, and when the fruit is ripe or fully ripened, it is picked and dried in the sun.

Actinomycetes are known as lignans such as actigenein, lignan glycosides actin and caffeic acid glycosides.

It is used as an anti-inflammatory agent for skin diseases such as inflammation and drainage, and is naturally grown in Korea and China.

Glycyrrhizae Radix is a perennial plant belonging to the soybean family, and is the root of the European licorice (Glycyrrhiza glabraL.), Manchuria licorice (Glycyrrhiza uralensis F.) or other herbaceous plants.

The pharmacological effects of licorice include adrenocorticotropic action, antiinflammatory activity and antiallergic action, action on digestive system, detoxification action, action on lipid metabolism, effect on experimental jaundice, vasopressin action, analgesic and antiepileptic action, And the effects on the reproductive system, antitumor action, other body weight gain, muscle strength, hypertension, hemolysis, insecticidal action, antimicrobial action, antipyretic action,

Medicinally, mainly roots and apricot are used. The main ingredient used is triterpenoid, glycyrrhizic acid (saponin) in the range of 3.63 to 13.06%, and various kinds of flavone compounds (Genetic Medicine Manual, Genetic Medicine, Revised 3rd Edition (2002), Tongmyung).

Paeoniae (Paeoniae Radix) is one of the most important medicinal herbs as a root of the medicinal herb and its plants.

Leaves are alternate phyllotaxis, petiole is long, 2 times exudate leaf, small leaf is oval, no sawteeth, flower blooms one at the end of stem, white flowers bloom in 5th and 6th month.

Herbal medicine is dried root, and the plant grows in the shade of a mountain tree.

It is distributed all over Korea. In one branch, it is collected in March and August and dried in the sun to be used for alveolar, hematocrit, blood, ejaculation, and till. In one room, it has been used as convergence relief, epidermis, and analgesic.

Morus alba, Linus, Morus alba for Peldulls DIPPEL, Morus bombycis, Koidzumi, Morus bombycis and Ramulus Mori are the dried branches of the mulberry plants. ), Morus bombycis var. Caudatifolia, Morus bombycis var. Maritima KOIDZ, Morus bombycis for Kase VYKEI, Mongolian mulberry, Royal mulberry, Morus tiliaefolia, Makino , These are deciduous broad - leaved arboreous trees distributed horizontally at altitudes of 500 - 1,400 m in most parts of Korea, and they grow in the vicinity of mountain villages or at the foot of mountains.

Generally, the upper limbs are slightly bitter, and they have been used as herbal medicines for a long time because of their efficacy such as anti-inflammation, diuretic, tonic, sweating and removing the wind (Korean Patent No. 230130).

Plum tree (Prunus mume Sieb.Et Zucc) is a small leafy tree (small tree) with oval leaves staggered, and before the early spring leaves, white or red The color blossoms.

 The fruit of the plum tree is called the plum, and the fruit is round and yellow when worn.

 There are lemon, malic acid, vinegar, succinic acid, sitosterol, oleic acid, cetyl alcohol and seed amidgalin in the fruit flesh and also used as a raw material of lemon acid. Dried fruit contains 19% of lemon acid and 1.5% of malic acid. You can make lemon acid with a 10% buildup rate.

In migraine treatment, it is used as a calming concentrate, diarrhea, fever, cough medicine, and phlegm (herbal ingredients and use, January, 1999, 358).

Togi (peach branch) is a peach tree branch. Peach tree is a small tall tree with leaves.

Flowers contain chemperol, its glycosides, tribasic acid and dioxybasic acid, leaves contain nitrile glycosides, and when steam is distilled, benzaldehyde and hydrogen cyanide are formed and 0.07-0.2% tannins are contained.

(C ₁₅ H ₁₂ O ₅ ), triacontane, pentatriacontane, etc., and the oil content of oil 40 to 50% ( The main components are olein, palmitin, stearin).

Flowers are used as a diuretic or synergistic therapy in constipation therapy. In the private sector, the leaves are put into a kiln, and the bath is bathed in raisins and eczema.

Jiji (槐 枝) is a branch of a pictorial tree. Sophora Japonica L belongs to the legume, and it is distributed in Korea, Japan, and China, about 25m high.

The branch is spread, the small branch is green, and it is characterized by the smell when it is cut. The leaf is alternate and it is once folded compound leaf.

It is said that it is effective to wash the branches and branches of the painting with water when it is damp and the under side of the scrotum and the scrotum are itchy.

The flower bud of a painting tree is called a weeping or gnawing, and the fruit is called a gnarrow or a sacrum.

Fruits are rounded or rosary in shape and fruits are used in the treatment of hemorrhoids along with branches and bark, and garden or wood is also used as furniture.

On the other hand, a known use of beak is to treat or prevent arteriosclerosis and hypertension, and may be used to make beer and paper yellow (Korean Patent Registration No. 2011-0004603).

Salix koreensis Andersson is also known as willow or weeping will, and the shell contains 3 to 10% of tannin, flavon, ascorbic acid, glycoside salicin (C ₁₃ H ₁₈ O ₇ , Point 201.5 ° C, unrolled in water, slightly loosened in alcohol, not dissolved in ether).

Salicin is absent or contains up to 5% depending on the area and type of plant.

Salicin is degraded by enzymes to nin and glucose.

Willow has a component (salicyl) that is similar to the main active ingredient of aspirin, so it acts to relieve fever and relieve inflammation and pain.

Therefore, it has a good effect to alleviate the mild symptoms accompanied by fever like cold body aches and pain caused by acute chronic rheumatism and mild symptoms of headache and inflammation, and when it is best when it is harvested between February and March It uses bark.

Examples of technologies utilizing the efficacy of such willow are Korean Patent Application No. 10-2008-7000798, "Willow tree extract, its use, and formulation containing it", Korean patent application No. 10-2001-0081882 "Willow tree extract And a hair cosmetic composition for preventing dandruff.

Bamboo is a generic term for perennial plants belonging to the bamboo family (Bambusoideae) belonging to the monocotyledonous plant (Poaceae). It grows mainly in the tropics, especially in the seasonal area of Asia. In Korea, It is widely distributed.

Bamboo is widely used as a medicinal product from root to leaf. It is known that the gibbons taste in the ancient literature <Shin Bong Intersection> is effective for the above-mentioned, tumor, fever and confusion. Especially, bamboo oil (bamboo oil) , Acne, eczema, athlete's foot, stinging, itching of the scalp, hair loss, psoriasis, and the like.

In addition, the contents recorded in medical books are known to have a mysterious effect on kwakran, gyeolgi, diabetes, headache, hypertension, dizziness, nervous breakdown, pregnancy anemia, epilepsy, insomnia, excessive drinking and fatigue recovery.

In order to utilize the good efficacy of bamboo, various foods using bamboo barrels have been developed. However, there is only bamboo tea using bamboo leaves.

Examples of techniques using the bamboo effect include 'whitening cosmetic ingredient containing bamboo extract' (Korean Patent Application No. 2000-0007943) and 'cosmetic composition for improving acne skin' (Korean Patent Application No. 10-2011-0068203) , A novel therapeutic composition containing bamboo extract, and a therapeutic agent containing the composition (Korean Patent Application No. 10-2000-0052649).

In the present invention, the above-mentioned ingredients are used in an amount of 5 to 22% by weight, 1 to 15% by weight of white paper, 1 to 15% by weight of citron, 1 to 15% by weight of Puerariae, 5 to 22% 1 to 15% by weight of the top layer, 5 to 40% by weight of the top layer, 1 to 15% by weight of the magazine, 1 to 15% by weight of the topical composition, 1 to 15% To produce a fermented extract.

The present invention relates to a cosmetic composition for improving atopic skin comprising a bamboo fermentation extract prepared by the above method in an amount of 0.001 to 20% by weight, preferably 0.1 to 10% by weight, as an active ingredient in cosmetics.

The present invention is a natural fermented herbal medicine extract obtained by fermenting a natural herbal medicine extract in a bamboo barrel and having excellent moisturizing effect, anti-inflammatory effect and free radical scavenging effect, and is excellent in improvement of atopic skin.

The present invention relates to a bamboo plant which is capable of improving atopic skin due to its moisturizing effect, anti-inflammatory effect and free radical scavenging effect, and which can improve atopic skin, bamboo plant, And to provide a cosmetic composition containing a herbal fermented extract.

Specifically, the method for preparing the fermented extracts of the present invention is as follows.

1 to 22% by weight of ground wax, 1 to 15% by weight of white wax, 1 to 15% by weight of royal wax, 1 to 15% by weight of Puerariae, 1 to 22% 1 to 40% by weight of maggie, 1 to 15% by weight of maggie, 1 to 15% by weight of doze, 1 to 15% by weight of starch and 1 to 40% by weight of oil.

The components were extracted with a solvent, filtered through a filter paper having a pore size of 0.45 mu m, and then concentrated under reduced pressure at a temperature of about 60 DEG C before and after the filtration. The filtrate was added to a bamboo container and sterilized at about 110 to 130 DEG C, (BD Difco) for 18 hours at 26 ° C. Saccharomyces cerevisiae culture medium, and fermented at 20 to 30 DEG C for 1 to 7 days.

As the solvent, one or two or more solvents selected from the group consisting of purified water, ethanol, butylene glycol, propylene glycol, glycerin and methanol are used.

As the extraction method, extraction is carried out by heating at 50 to 95 ° C for 4 to 20 hours in a state where a cooling condenser is installed to prevent the solvent from being evaporated, or it is immersed in a solvent at 5 to 37 ° C for 1 to 15 days to extract an active ingredient Method can be used.

The bamboo fermentation extract prepared in the above manner is filtered through a filter paper having a pore size of 0.45 m and then added to the cosmetic in a liquid form in an amount of 0.001 to 20% by weight, preferably 0.1 to 10% by weight to prepare the cosmetic composition of the present invention .

If the content is out of the lower limit of the above range, the effect is remarkably reduced. If the content is out of the upper limit, it is difficult to prepare cosmetic formulations, or is not preferable from the economical point of view.

The cosmetic of the present invention may be used as formulations such as lotion (skin lotion), nutrition lotion, nutritional cream, massage cream, nutritional essence, cleanser and the like, and is not limited thereto and may be used for other hair or skin.

Hereinafter, the present invention will be described in more detail with reference to the following examples and experimental examples, but the present invention is not limited thereto.

Example  1-4. Purified water  Used plant extract filtration Concentrate  Produce

The mixture is mixed as shown in the following table and then aged at 25 ° C for 7 days using an extractor using purified water.

After aging, the mixture was filtered through a 400 mesh filter cloth, filtered through a filter paper having a pore size of 0.45 mu m, and concentrated under reduced pressure at 60 DEG C, and the results are shown in Table 1 below.

[Table 1]

Example  5. 70% functional ethanol extract Concentrate  Produce

The mixture was mixed with 14 kg of 70% aqueous ethanol at 25 ° C, and the mixture was stirred at 25 ° C for 10 minutes. Mature for 7 days.

After aging, the mixture was filtered through a filter paper having a pore size of 0.45 mu m and concentrated under reduced pressure at 60 DEG C, and the results are shown in Table 2 below.

[Table 2]

Example  6, 7 and Comparative Example  Using bamboo containers from 1 to 3 Fermentation product  Produce

The concentrates of Examples 1 to 5 were added to a bamboo container and sterilized at 121 ° C for 30 minutes. After cooling to 25 ° C, yeast strain (Sabouraud dextrose broth (BD Difco)) cultured for 18 hours at 26 ° C Saccharomyces cerevisiae were inoculated in 1/100 volume of the concentrated liquid. The fermented broth was fermented at 26 캜 for 3 days, and the fermented broth was prepared.

[Table 3]

Comparative Example  4. Using a glass incubator Fermentation product  Produce

500 g of the concentrate of Example 1 was added to a 2 L Erlenmeyer flask and sterilized at 121 占 폚 for 30 minutes. After cooling to 25 占 폚, yeast strain Saccharomyces cultured for 18 hours at 26 占 폚 using abouraud dextrose broth (BD Difco) cerevisiae culture medium was inoculated at a weight of 1/100 of the concentrated liquid amount. The fermented broth was fermented at 26 캜 for 3 days after aging, and the results are shown in Table 4.

[Table 4]

Experimental Example  One. Free radical  Erasure efficacy experiment

Embodiment, the free radical scavenging activity was measured are shown, based on the solids content of the SC ₅₀ having an example value of each of the embodiments shown in Table 4.

Experimental Method

The free radical scavenging activity was determined by the method of Fujita et al. Using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH).

2 ml of each sample was added to 1 ml of a solution of 0.2 mM of 1,1-diphenyl-2-picrylhydrazyl (DPPH, Sigma, USA), and the reaction was carried out at room temperature for 10 minutes. Then, the absorbance was measured at 525 nm And the free radical scavenging effect (%) was obtained.

Blank used ethanol instead of DPPH, and 2 mL of ascorbic acid (L-ascorbic acid) was added to the control group.

The free radical scavenging activity SC ₅₀ of each of the Examples and Comparative Examples was determined and shown in Table 5.

The free radical scavenging activity SC ₅₀ represents the concentration (쨉 g / ml) of the extract required for 50% free radical scavenging, and the results are shown in Table 4.

[Table 5]

In Table 5, the purified water extracted bamboo tuber fermented extract of the present invention (Example 6) contained free radical scavenging activity SC ₅₀ (7.5 ㎍ / ㎖) and 70% ethanol extract of bamboo tuber fermentation extract (Example 7) showed similar results as 7.3 ㎍ / ㎖. This shows that the free radical scavenging effect is excellent even in a small amount as compared with the ascorbic acid used as a control.

In addition, the free radical scavenging effect is superior to Comparative Examples 1 to 3 outside the composition range of the present invention, and the fermentation extract using the bamboo barrel (Example 6, Example 7) is superior to the general container fermentation extract (Comparative Example 4) Indicated.

Therefore, it can be seen that the bamboo fermented extract of the present invention is excellent in free radical scavenging effect.

Experimental Example  2. Anti-inflammatory experiment

In order to investigate the anti-inflammatory effect of the above examples, a method using an arachidonic acid (A. Crummey, GP Harper, EA Boyle and FR Mangan, Inhibition of arachidonic acid-induced ear edema as a model for assessing topical anti-inflammatory compound. Agents and Actions, 1987, 20: 69-76).

 Experimental Method

The left ear of the mouse was used as a control site and the right ear was used as a test site. The ear was cleanly washed with ethanol before the sample was applied, and 20 μl of the sample of each example was continuously applied once a day for 4 days and 1 hour after the last application The left ear was coated with ethanol and the right ear was coated with arachidonic acid (2 mg / ear). After 1 hour, ear edema was repeatedly measured three times with micrometer in each ear.

The same method was repeatedly measured with the extracts of Examples 1 to 7 and Comparative Examples 1 to 4.

The anti-inflammatory effect was judged to be suppression of edema based on arachidonic acid treatment group. The results are shown in Table 6.

 ※ Inhibition rate (%) = (A - B) / A × 100

A: Mean thickness of control ear (thickness of ear treated with arachidonic acid - thickness of untreated ear of arachidonic acid)

B: Thickness of the coated ear of the embodiment (thickness of the sample treated ear - thickness of the untreated sample)

[ Table 6]

As shown in Table 6, the 1.0% sample of the bamboo fermented product of the present invention exhibited an inhibition rate of inflammation of 32.1%, and the 1.0% sample of the 70% ethanol extract of the bamboo fermented product had an inflammation inhibition rate of 34.1% These results are remarkably improved compared to the comparative examples which are outside the scope of the present invention. These values are superior to those of indomethacin, which is an excellent anti-inflammatory substance as a pharmaceutical ingredient. .

Therefore, it can be seen that the bamboo fermented extract of the present invention is excellent in anti-inflammatory effect.

Experimental Example  3. Moisture Experiment

In order to confirm the moisturizing effect of the examples of the present invention, the moisturizing effect was measured using a Corneometer CM 825 (Courage + Khazaka, Cologne, Germany).

For each of the examples, the solid content was fixed at 1%, and the sample was diluted with the extraction solvent. The moisture content was measured at 40 minutes after application of the sample.

Experimental Method

Twenty women in their twenties underwent 2 × 2 ㎠, 8 compartments on the upper abdomen and washed several times with water, and then the water was removed without stimulation.

After 5 minutes, 10 μl of the measurement sample is applied to each of the two compartments, and uniformly applied.

After 10 minutes, measure 5 times per compartment using a corneometer.

One compartment was measured 6 times at intervals of 5 minutes, and the room temperature was 20 ° C and the relative humidity was 20%.

Water contents (%) = Corneometer value after application of sample - Corneometer value before application of sample, data value is average value of 20 persons.

[Table 7]

As a result, the moisturizing effect of the non-fermented extracts was also shown, but it was found that the fermented bamboo fermentation extract of the present invention exhibited a better moisturizing effect than the fermented extract of general containers and the fermented non-fermented extracts.

The purified water extracted bamboo fermented product of Example 6 and the 70% ethanol extracted bamboo fermented product of Example 7 showed similar effects.

Prescription example  Criteria for dispensing

On the basis of the results measured in Comparative Examples 1 to 4, the bamboo fermented products of Examples 6 and 7 exhibited comparatively similar results and showed high overall effectiveness. Example 7. The content of each component is% by weight.

Prescription example  One.

Examples of prescription of cosmetic lotion (skin lotion) among cosmetic compositions containing Example 7 are as follows.

<Manufacturing Method>

Formulation example 1: In step 11, 2, 3, 4, and 8 are added in this order and dissolved by stirring. After 5 times dissolving by heating at 60 ° C, 10 times are added and stirred at 11 times .

Finally, 1, 6, 7, and 9 are added, agitated sufficiently after stirring.

Comparative Formulation Example 1: Prepared in the same manner as in Formulation Example 1 except that Formulation Example 1 was omitted.

Prescription example  2.

Examples of prescription of nutritional lotion among cosmetics containing Example 7 are as follows.

<Manufacturing Method>

Formulation Example 2: 10, 11, 13 and 16 were mixed and stirred while being heated to 80 to 85 ° C, charged into the production section, and then emulsified with 2, 3, 4, 5, 6, 7, , 12 is heated to between 80 and 85 캜 and then emulsified.

After the emulsification is completed, the mixture is cooled to 50 캜 with stirring using an agitator, and then the mixture is cooled to 45 캜 and then added to 35 캜. The mixture is cooled to 25 캜 and aged.

Comparative Formulation Example 2: Prepared in the same manner as in Formulation Example 2, except that Formulation Example 2 is omitted.

Prescription example  3

Examples of prescription examples of the nutritional cream among the cosmetics containing Example 7 are as follows.

<Manufacturing Method>

Formulation Example 3: 10, 12, and 15 were heated to between 80 and 85 캜 with stirring and mixing, and then put into a manufacturing part, and then an emulsifying agent was allowed to react and 2,3,3,4,5,6,7,8,9,11 Is heated to 80 to 85 DEG C and then emulsified.

After the emulsification is completed, the mixture is cooled to 50 ° C with stirring using an agitator, and then the mixture is cooled to 45 ° C and then cooled to 45 ° C. After cooling to 35 ° C, the mixture is cooled to 25 ° C It ages.

Comparative Formulation Example 3: Formulation Example 3 is prepared in the same manner as in Formulation Example 3 except that Example 7 is omitted.

Prescription example  4.

An example of the formulation of the massage cream in the cosmetic containing Example 7 is as follows.

<Manufacturing Method>

Formulation Example 4: 8, 9, 10, 12, and 15 were mixed and stirred while heating to 80 to 85 ° C, Is heated to 80 to 85 DEG C and then emulsified.

After completion of the emulsification, the mixture was cooled to 50 캜 with stirring using an agitator, and then the mixture was cooled to 45 캜 and then cooled to 45 캜. After cooling to 35 캜, the mixture was cooled to 25 캜 It ages.

Comparative Formulation Example 4: Formulation Example 4 is prepared in the same manner as in Formulation Example 4 except that Example 7 is omitted.

Prescription example  5.

Examples of prescription of nutritional essence among cosmetics containing Example 7 are as follows.

<Manufacturing Method>

Formulation Example 5: Raw materials 2, 3, 4, 5 and 6 are homogenized at a constant temperature to form nonionic amphipathic lipids.

The non-ionic amphiphilic lipids and the raw materials 1, 7, 8 and 14 were mixed, homogenized at a constant temperature, passed through a microfluidizer, and then the raw material 9 was gradually added at a constant temperature to homogenize After passing again through a microfluidizer, 10, 11, 12 and 13 are added, dispersed, stabilized and aged.

Comparative Formulation Example 5: Prepared in the same manner as in Formulation Example 5 except that Formulation Example 5 was omitted.

Prescription example  6

An example of the formulation of the emulsion type foundation among the cosmetics containing Example 7 is as follows.

<Manufacturing Method>

Formulation Example 6: 9, 10, 11, 12, 13, 14, 15, and 17 were heated to 80 to 85 ° C while stirring and mixing, , 6, 7, and 8 are heated to between 80 and 85 캜 and then emulsified.

After the emulsification is completed, the mixture is cooled to 45 ° C with stirring using an agitator, and then 16 times. After cooling to 35 ° C, the mixture is cooled to 25 ° C and aged.

Comparative Formulation Example 6: The same procedure as in Formulation Example 6 was carried out except that in Formulation Example 6, Example 7 was omitted.

Test Example  1. Atopy test

The following clinical confirmation tests were carried out to confirm the effect of atopic dermatitis on the nutritional cream of Formulation Example 3 and Comparative Formulation Example 3 when applied to the skin.

Table 8 shows the atopy improvement effect of about 20 out of the patients suffering from atopic dermatitis for 2 years or more as a 5 to 30 year old atopic patient.

To the same person, the nutritional cream of Formulation Example 3 was applied to the left hand, and the nutritional cream of Comparative Formulation Example 3 was applied to the right hand of the abdomen every evening, and the skin was applied to the skin once a day for 60 days to confirm improvement of atopic condition The degree of atopic condition improvement was measured.

[Table 8]

It can be seen that Formulation Example 3 prepared by adding Example 7, as in the above-mentioned investigation, is superior to Comparative Formulation Example 3 in which Example 7 is not added.

These results indicate that the selection of medicinal herbs useful for atopic skin and the fermentation of bamboo containers in the present invention enable extraction of the medicinal ingredients contained in bamboo and the use of natural bamboo containers as fermentation containers, Therefore, it is possible to ferment without separate nutrient medium, and there is no fear of secondary contamination because no separate incubator is used.

The bamboo fermented extract of the present invention is an extremely useful invention as a raw material for cosmetics since it has an excellent atopic skin improving effect and is harmless to human body.

The detailed description of the invention is merely exemplary of the invention, which is used for the purpose of describing the invention only and is not used to limit the scope of the invention as defined in the claims or the claims.

Therefore, those skilled in the art will appreciate that various modifications and equivalent embodiments are possible without departing from the scope of the present invention.

Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

Claims (7)

5 to 22% by weight of fruit fat with anti-inflammatory, moisturizing and free radical scavenging effect, 1 to 15% by weight of white paper, 1 to 15% by weight of celestial root, 1 to 15% by weight of brown root, 5 to 22% by weight of bridge, 5 to 22% of licorice Medicinal raw material consisting of%, 1 to 15% by weight, 5 to 40% by weight, 1 to 15% by weight, 1 to 15% by weight of paper, 1 to 15% by weight of raw paper, 5 to 40% by weight of fat and oil as solvent Extraction, cosmetic composition for atopic skin improvement comprising the fermented extract fermented in a bamboo barrel as an active ingredient. The cosmetic composition for improving atopic skin according to claim 1, wherein the solvent is at least one solvent selected from the group consisting of purified water, ethanol, butylene glycol, propylene glycol, glycerin, and methanol. The extraction method according to claim 1, wherein the extraction method comprises heating at 50 to 95 ° C for 4 to 20 hours in a state where a cooling condenser is installed to prevent evaporation of the solvent, or the extraction is carried out at 5 to 37 ° C for 1 to 15 days A cosmetic composition for improving atopic skin characterized by extracting an active ingredient. According to claim 1, wherein the fermentation method extracts yeast ( Saccharomyces) cerevisiae ) using a bamboo barrel container aged 1 to 7 days of fermentation, characterized in that the cosmetic composition for skin improvement. The cosmetic composition for improving atopic skin according to claim 1, wherein the bamboo tubular fermented extract is contained in an amount of 0.001 to 20 wt% based on the weight of the liquid phase. The cosmetic composition for improving atopic skin according to claim 1, wherein the cosmetic composition is a lotion (skin lotion), a nutrient lotion, a nutritional cream, a massage cream, a nutritional essence, or a cleanser formulation. 5 to 22% by weight of fibrils having anti-inflammatory, moisturizing and free radical scavenging effects, 1 to 15% by weight of white paper, 1 to 15% by weight of cilantro, 1 to 15% by weight of Puerariae, 5 to 22% By weight, 1 to 15% by weight of peanuts, 5 to 40% by weight of topsoil, 1 to 15% by weight of magazine, 1 to 15% by weight of doze, 1 to 15%
The mixed components are extracted with a solvent, filtered through a filter paper having a pore size of 0.45 mu m, and concentrated under reduced pressure at a temperature of about 60 DEG C before and after the filtration.
The extract was added to a bamboo vial, sterilized at about 110 to 130 DEG C, cooled, and the yeast ( Saccharomyces cerevisiae ), aging fermentation at 20 to 30 DEG C for 1 to 7 days,
Wherein the fermented bamboo extract is filtered through a filter paper having a pore size of 0.45 μm and then added to the cosmetic composition in a liquid form in an amount of 0.1 to 10% by weight.