CN109182405A - A method of γ-polyglutamic acid is produced using lichens bud pole bacterium solid state fermentation - Google Patents
A method of γ-polyglutamic acid is produced using lichens bud pole bacterium solid state fermentation Download PDFInfo
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Abstract
γ-polyglutamic acid method is produced using lichens bud pole bacterium solid state fermentation the present invention relates to a kind of, the steps include: that being inoculated with lichens bud pole bacterium after solid-state fermentation culture medium sterilizes stands solid state fermentation, produces γ-polyglutamic acid；Solid-state fermentation culture medium includes solid fermentation base-material and nutritional supplement, and solid fermentation base-material includes dregs of beans and natural porous carrier.The present invention is done using dregs of beans and natural minerals as solid state fermentation base-material, it is mixed into a certain proportion of nutrient solution, it is inoculated with lichens bud pole bacterium, γ-polyglutamic acid method is produced by solid state fermentation, the comprehensive exploitation of dregs of beans, higher value application is promoted and reduces production cost.
Description
Technical field
The invention belongs to technical field of biological fermentation, are related to a kind of poly- using lichens bud pole bacterium solid state fermentation production γ-
The method of glutamic acid.
Background technique
γ-polyglutamic acid, molecular weight have application in 10-1000kDa various fields, can use in terms of food
In antifreeze, thickener, except astringent etc., can be used for moisture saver mask etc. in cosmetic industry, in pharmaceuticals industry, can be used as medicine
Object carrier, adhesive etc. have preferable water-retaining property to edaphophyte, also can be used as fertilizer synergist etc., in industry in agricultural
On can be used for high-molecular biologic flocculant, heavy metal absorbent etc., it can thus be seen that γ-polyglutamic acid many fields all
It has a good application prospect.
γ-polyglutamic acid the production method studied at present is mostly liquid fermentation, not yet finds the side for using carrier adsorption
Formula produces γ-polyglutamic acid method by matrix solid state fermentation of dregs of beans.Solution fermentation is at high cost, and fermentation process is by dissolved oxygen
With the limitation of concentration of substrate, oxygen transfer low efficiency is passed, therefore solid state fermentation has very high application prospect.
Summary of the invention
It is poly- using lichens bud pole bacterium solid state fermentation production γ-that technical problem to be solved by the invention is to provide a kind of
The method of glutamic acid.The present invention is done using dregs of beans, natural minerals as solid state fermentation base-material, and a certain proportion of nutrient solution is mixed into,
It is inoculated with lichens bud pole bacterium, γ-polyglutamic acid method is produced by solid state fermentation, promotes comprehensive exploitation, the high level of dregs of beans
Change and utilizes and reduce production cost.
To solve the above problems, the present invention adopts the following technical scheme:
A method of γ-polyglutamic acid being produced using lichens bud pole bacterium solid state fermentation, after solid-state fermentation culture medium is sterilized
It is inoculated with lichens bud pole bacterium and stands solid state fermentation, produce γ-polyglutamic acid；The solid-state fermentation culture medium includes solid fermentation
Base-material and nutritional supplement, the solid fermentation base-material include dregs of beans and natural porous carrier.
Preferably, the natural porous carrier is the natural peral rock or vermiculite that aperture is 3-6mm.
Preferably, the nutritional supplement is glucose, sodium glutamate, K2SO4、(NH4)2SO4, anhydrous CaCl2、FeSO4、
MgSO4Formed with water, their thousand points of concentration of weight respectively are, 60-100 ‰, 40-80 ‰, 10-20 ‰, 5-10 ‰,
Remaining is water by 0.5-1 ‰, 0-0.1 ‰, 0-0.5 ‰, and pH value is 7.0-7.2.
Preferably, in the solid fermentation base-material, the mass ratio of natural porous carrier and dregs of beans is 1-3:7-9, described solid
The mass ratio of body fermentation base-material and nutritional supplement is 1:1.0-1.5.
Preferably, the preparation step of the solid-state fermentation culture medium are as follows: vermiculite will be sieved, and be mixed after dregs of beans grinding and sieving
It is even to be used as solid fermentation base-material；Nutritional supplement is added in solid fermentation base-material and is uniformly mixed, solid fermentation culture medium is obtained.
Preferably, the sterilizing methods of the solid-state fermentation culture medium are solid fermentation culture medium, are packed into polyethylene plastic bag
In, tighten sack, in 105 DEG C boiling 20 minutes, sterilizing.
Preferably, the inoculum concentration of the lichens bud pole bacterium is 5% -10%, cold after the sterilizing of solid-state fermentation culture medium
It is inoculated with when but to 50 DEG C.
Preferably, the standing solid state fermentation are as follows: after the sterilizing of solid-state fermentation culture medium, when being cooled to 50 DEG C by
5-10% (V/W) is inoculated with lichens bud pole bacterium in solid-state fermentation culture medium, is sufficiently stirred, strain is made to be uniformly distributed in culture medium
Surface, the incubator after inoculation keep 35-37 DEG C of constant temperature, and humidity is strictly maintained at 50% or more in incubator, and ferment 3-6
It.
Preferably, stand solid state fermentation after the completion of, extract γ-polyglutamic acid the step of are as follows: be added solid fermentation object 5-
The water of 10 times of volumes stirs 1-2 hours, extracts the γ-polyglutamic acid of lichens bud pole bacterium secretion in the fermentation medium, warp
After crossing 100-200 mesh net filtrations, filtrate is collected, filtrate concentrated by rotary evaporation to 20-60g/L of concentration adds 1-4 times of volumes
Ethyl alcohol, stand 6-48 hours, precipitating be collected by centrifugation, then with the ethanol washing of 1-4 times of volumes, vacuum freeze drying is obtained
To purification γ-polyglutamic acid.
Principle: 1, solid absorption fermentation method is fermented using good adsorbent carrier, compared to traditional solid state fermentation side
Method can ratio in more specific control reactant between each component, preferably obtain higher yield, what the present invention used
Perlite vermiculite is the water absorbing properties that there is natural minerals itself same quasi-mineral not have, it is different from dregs of beans and ingredient
Nutrient solution combination can preferably improve dissolved oxygen content, propose high heat and mass transfer efficiency, easy to operate, cost is very low.
γ-polyglutamic acid molecular weight of this method production can be applied to agriculture field between 1000-5000kDa,
Such as fertilizer synergistic, soil water-retaining moisturizing, balanced soil pH value.
Beneficial effect
1. the present invention is fermentation base-material with dregs of beans, natural carrier, addition-certainty ratio nutrient solution are carried, solid state fermentation γ-gathers
Glutamic acid provides-kind efficient mass production γ-polyglutamic acid method, while promoting the comprehensive exploitation of dregs of beans, high level
Change and utilizes and reduce production cost.
2. being equipped with natural carrier, compared to chemically synthesized carrier is utilized, it is applied in agriculture field in following process,
With natural free of contamination effect, it may be directly applied to farmland production, there is the effect of moisturizing synergy to soil.
Detailed description of the invention
Fig. 1 is the process flow that the present invention produces γ-polyglutamic acid method using lichens bud pole bacterium solid state fermentation
Figure.
Specific embodiment
The invention will be further elucidated with reference to specific embodiments.It should be understood that these embodiments are merely to illustrate this hair
It is bright rather than limit the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, art technology
Personnel can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Fixed range.
Embodiment 1
A method of γ-polyglutamic acid being produced using lichens bud pole bacterium solid state fermentation, as shown in Figure 1, the steps include:
The activation and culture of bacillus licheniformis.Primary inclined plane uses the inclined-plane LB, for 24 hours in 37 DEG C of cultures, is inoculated into seed culture
In base, 37 DEG C of shaking flask cultures, 12-18 hours extremely growth logarithmic phases are spare as solid fermentation strain
Using 800g dregs of beans and 200g perlite as solid fermentation base-material；Separately by glucose 90g, sodium glutamate 80g, K2SO4
15g 、(NH4)2SO4 10g, anhydrous CaCl2 0.5g FeSO4 0.1g 、MgSO4 0.5g, with HCl or NaOH aqueous solution tune pH
Value is 7.0；Perlite is sieved, the aperture of perlite porous carrier is 3-6mm；It is mixed after dregs of beans grinding and sieving and is used as solid
Ferment base-material；Above-mentioned solid fermentation base-material is mixed into nutritional supplement, the two is mixed into solid according to the weight ratio of 1:1
Fermentation medium is fitted into polyethylene plastic bag, tightens sack, in 105 DEG C boiling 20 minutes, 50 DEG C are cooled to, according to 10%
Ratio is seeded in above-mentioned solid fermentation culture medium, after mixing evenly, is laid in pallet by the thickness of 3cm, is covered with gauze,
In 37 DEG C, solid fermentation 3 days, ambient humidity turned daily 50% or more, was uniformly mixed culture
Fermentation 4 days after, in culture medium occur can wire drawing stick substance, take out 10g substance, be dissolved in shaking flask with the distilled water of 10 times of weight
In, 150 turns, 1h, 200 mesh net filtrations, collecting filtrate, " filtrate rotates to γ-polyglutamic acid concentration in 40-60g/L, stops
The ethyl alcohol of 4 times of volumes is added into concentrate, is sufficiently stirred for spin-ended steaming, stands 6-48 hours and precipitating is collected by centrifugation, 4800
Rev/min centrifugation 10 minutes, what is obtained is precipitated as γ-polyglutamic acid semifinished product.
The ethanol washing of 1-2 times of volume of obtained γ-polyglutamic acid semifinished product is precipitated, filters and recycles, at liquid nitrogen
Reason grinding, freeze-drying obtain γ-polyglutamic acid highly finished product, γ-polyglutamic acid yield of per kilogram raw material dry matter is
186g。
Embodiment 2
A method of γ-polyglutamic acid being produced using lichens bud pole bacterium solid state fermentation, the steps include: bacillus licheniformis
Activation and culture with embodiment one.
Using 800g dregs of beans and 200g vermiculite as solid fermentation base-material；Separately by glucose 135g, sodium glutamate 120g,
K2SO422.5g 、(NH4)2SO4 15g, anhydrous CaCl2 0.75g FeSO4 0.15g 、MgSO4 0.75g, preparation become
1500mL nutritional supplement is 7.0 with HCl or NaOH aqueous solution tune pH value；Vermiculite is sieved, the aperture of vermiculite porous carrier is
3-5mm；It is mixed after dregs of beans grinding and sieving and is used as solid fermentation base-material；Above-mentioned solid fermentation base-material is mixed into nutritional supplement,
The two is mixed into solid fermentation culture medium according to the weight ratio of 1:1.5, is fitted into polyethylene plastic bag, tightens sack,
In 105 DEG C boiling 20 minutes, be cooled to 50 DEG C, be seeded according to 7.5% ratio in above-mentioned solid fermentation culture medium, stir
After uniformly, be laid in pallet by the thickness of 3cm, covered with gauze, in 37 DEG C, solid fermentation 6 days, ambient humidity 50% or more,
It turns daily, is uniformly mixed culture.
Fermentation 6 days after, in culture medium occur can wire drawing stick substance, take out 10g substance, be dissolved in the distilled water of 10 times of weight
In shaking flask, 150 turns, 1h, 200 mesh net filtrations collect filtrate, and filtrate rotates to γ-polyglutamic acid concentration in 40-60g/L
When, stop revolving, the ethyl alcohol of 4 times of volumes be added into concentrate, is sufficiently stirred, stands 6-48 hours and precipitating is collected by centrifugation,
4800 revs/min are centrifuged 10 minutes, and what is obtained is precipitated as γ-polyglutamic acid semifinished product.
The ethanol washing of 2 times of volumes of obtained γ-polyglutamic acid semifinished product is precipitated, recycling, liquid nitrogen processing are filtered
Grinding, freeze-drying, obtains γ-polyglutamic acid highly finished product, γ-polyglutamic acid yield of per kilogram raw material dry matter is
232g。
Embodiment 3
A method of γ-polyglutamic acid being produced using lichens bud pole bacterium solid state fermentation, the steps include: bacillus licheniformis
Activation and culture with embodiment 1.
Using 900g dregs of beans and 100g vermiculite as solid fermentation base-material；Separately by glucose 150g, sodium glutamate 120g,
Yeast extract 40g, K2SO422.5g 、(NH4)2SO4 15g, anhydrous CaCl2 0.75g 、FeSO4 0.15g 、MgSO4 0.75g,
Preparing becomes 1500mL nutritional supplement, is 7.0 with HCl or NaOH aqueous solution tune pH value；Vermiculite is sieved, vermiculite porous carrier
Aperture be 4-6mm；It is mixed after dregs of beans grinding and sieving and is used as solid fermentation base-material；Above-mentioned solid fermentation base-material is mixed into nutrition
Replenishers, the two are mixed into solid fermentation culture medium according to the weight ratio of 1:1.5, are fitted into polyethylene plastic bag, prick
Tight sack, in 105 DEG C boiling 20 minutes, be cooled to 50 DEG C, be seeded according to 5% ratio in above-mentioned solid fermentation culture medium,
After mixing evenly, it is laid in pallet by the thickness of 3cm, is covered with gauze, in 37 DEG C, solid fermentation 4 days, ambient humidity was 50%
More than, it turns daily, is uniformly mixed culture
Fermentation 3 days after, in culture medium occur can wire drawing stick substance, take out 10g substance, be dissolved in shaking flask with the distilled water of 10 times of weight
In, 150 turns, 1h, 200 mesh net filtrations collect filtrate, and filtrate rotates to γ-polyglutamic acid concentration in 40-60g/L, stops
The ethyl alcohol of 4 times of volumes is added into concentrate, is sufficiently stirred for spin-ended steaming, stands 6-48 hours and precipitating is collected by centrifugation, 4800 turns/
Minute centrifugation 10 minutes, what is obtained is precipitated as γ-polyglutamic acid semifinished product.
The ethanol washing of 2 times of volumes of obtained γ-polyglutamic acid semifinished product is precipitated, recycling, liquid nitrogen processing are filtered
Grinding, freeze-drying, obtains γ-polyglutamic acid highly finished product, γ-polyglutamic acid yield of per kilogram raw material dry matter is
196g。
Embodiment 4
A method of γ-polyglutamic acid being produced using lichens bud pole bacterium solid state fermentation, the steps include: bacillus licheniformis
Activation and culture with embodiment 1.
Using 700g dregs of beans and 300g vermiculite as solid fermentation base-material；Separately by glucose 60g, sodium glutamate 120g, ferment
Female cream 40g, K2SO422.5g 、(NH4)2SO4 15g, anhydrous CaCl2 0.75g 、FeSO4 0.15g 、MgSO4 0.75g matches
It is made for 1500mL nutritional supplement, is 7.0 with HCl or NaOH aqueous solution tune pH value；Vermiculite is sieved, vermiculite porous carrier
Aperture is 4-6mm；It is mixed after dregs of beans grinding and sieving and is used as solid fermentation base-material；Above-mentioned solid fermentation base-material is mixed into nutrition to mend
Agent is filled, the two is mixed into solid fermentation culture medium according to the weight ratio of 1:1.5, is fitted into polyethylene plastic bag, tightens
Sack, in 105 DEG C boiling 20 minutes, be cooled to 50 DEG C, be seeded according to 10% ratio in above-mentioned solid fermentation culture medium,
After mixing evenly, it is laid in pallet by the thickness of 3cm, is covered with gauze, in 37 DEG C, solid fermentation 4 days, ambient humidity was 50%
More than, it turns daily, is uniformly mixed culture
Fermentation 3 days after, in culture medium occur can wire drawing stick substance, take out 10g substance, be dissolved in shaking flask with the distilled water of 10 times of weight
In, 150 turns, 1h, 200 mesh net filtrations collect filtrate, and filtrate rotates to γ-polyglutamic acid concentration in 40-60g/L, stops
The ethyl alcohol of 4 times of volumes is added into concentrate, is sufficiently stirred for spin-ended steaming, stands 6-48 hours and precipitating is collected by centrifugation, 4800 turns/
Minute centrifugation 10 minutes, what is obtained is precipitated as γ-polyglutamic acid semifinished product.
The ethanol washing of 2 times of volumes of obtained γ-polyglutamic acid semifinished product is precipitated, recycling, liquid nitrogen processing are filtered
Grinding, freeze-drying, obtains γ-polyglutamic acid highly finished product, γ-polyglutamic acid yield of per kilogram raw material dry matter is
140g。
Embodiment 5
A method of γ-polyglutamic acid being produced using lichens bud pole bacterium solid state fermentation, the steps include: bacillus licheniformis
Activation and culture with embodiment 1.
Using 700g dregs of beans and 300g vermiculite as solid fermentation base-material；Separately by glucose 60g, sodium glutamate 120g, ferment
Female cream 40g, K2SO422.5g 、(NH4)2SO4 15g, anhydrous CaCl2 0.75g 、FeSO4 0.15g 、MgSO4 0.75g matches
It is made for 1000mL nutritional supplement, is 7.0 with HCl or NaOH aqueous solution tune pH value；Vermiculite is sieved, vermiculite porous carrier
Aperture is 4-6mm；It is mixed after dregs of beans grinding and sieving and is used as solid fermentation base-material；Above-mentioned solid fermentation base-material is mixed into nutrition to mend
Agent is filled, the two is mixed into solid fermentation culture medium according to the weight ratio of 1:1, is fitted into polyethylene plastic bag, tightens bag
Mouthful, in 105 DEG C boiling 20 minutes, be cooled to 50 DEG C, be seeded in above-mentioned solid fermentation culture medium, stir according to 10% ratio
After mixing uniformly, be laid in pallet by the thickness of 3cm, covered with gauze, in 37 DEG C, solid fermentation 5 days, ambient humidity 50% with
On, it turns daily, is uniformly mixed culture
Fermentation 4 days after, in culture medium occur can wire drawing stick substance, take out 10g substance, be dissolved in shaking flask with the distilled water of 10 times of weight
In, 150 turns, 1h, 200 mesh net filtrations collect filtrate, and filtrate rotates to γ-polyglutamic acid concentration in 40-60g/L, stops
The ethyl alcohol of 4 times of volumes is added into concentrate, is sufficiently stirred for spin-ended steaming, stands 6-48 hours and precipitating is collected by centrifugation, 4800 turns/
Minute centrifugation 10 minutes, what is obtained is precipitated as γ-polyglutamic acid semifinished product.
The ethanol washing of 2 times of volumes of obtained γ-polyglutamic acid semifinished product is precipitated, recycling, liquid nitrogen processing are filtered
Grinding, freeze-drying, obtains γ-polyglutamic acid highly finished product, γ-polyglutamic acid yield of per kilogram raw material dry matter is
140g。
Comparative example 1
A method of γ-polyglutamic acid being produced using lichens bud pole bacterium solid state fermentation, the steps include: bacillus licheniformis
Activation and culture with embodiment one.
Using 1000g dregs of beans as solid fermentation base-material；Separately by glucose 135g, sodium glutamate 120g, yeast extract
40g、K2SO422.5g 、(NH4)2SO4 15g, anhydrous CaCl2 0.75g FeSO4 0.15g 、MgSO4 0.75g, preparation become
1500mL nutritional supplement is 7.0 with HCl or NaOH aqueous solution tune pH value；Solid fermentation substrate is used as after dregs of beans grinding and sieving
Material；Above-mentioned solid fermentation base-material is mixed into nutritional supplement, the two is mixed into solid fermentation according to the weight ratio of 1:1.5
Culture medium is fitted into polyethylene plastic bag, tightens sack, in 105 DEG C boiling 20 minutes, 50 DEG C are cooled to, according to 10% ratio
It is seeded in above-mentioned solid fermentation culture medium, after mixing evenly, is laid in pallet by the thickness of 3cm, is covered with gauze, in 37
DEG C, solid fermentation 6 days, ambient humidity turned daily 50% or more, was uniformly mixed culture
Fermentation 6 days after, in culture medium occur can wire drawing stick substance, take out 10g substance, be dissolved in shaking flask with the distilled water of 10 times of weight
In, 150 turns, 1h, 200 mesh net filtrations collect filtrate, and filtrate rotates to γ-polyglutamic acid concentration in 40-60g/L, stops
The ethyl alcohol of 4 times of volumes is added into concentrate, is sufficiently stirred for spin-ended steaming, stands 6-48 hours and precipitating is collected by centrifugation, 4800 turns/
Minute centrifugation 10 minutes, what is obtained is precipitated as γ-polyglutamic acid semifinished product.
The ethanol washing of 2 times of volumes of obtained γ-polyglutamic acid semifinished product is precipitated, recycling, liquid nitrogen processing are filtered
Grinding, freeze-drying, obtains γ-polyglutamic acid highly finished product, γ-polyglutamic acid yield of per kilogram raw material dry matter is
124g。
The embodiment of the present invention 1-5 illustrates the yield of the solid state fermentation base-material containing natural carrier more compared with comparative example 1
It is good.This is because the perlite vermiculite that the present invention uses is the water imbibition that there is natural minerals itself same quasi-mineral not have
Can, when individually dregs of beans being fermented, bulk is condensed into since the process dregs of beans of boiling can soften, thallus is inside it at this time
It is difficult to obtain sufficient oxygen, can preferably improve dissolved oxygen when combining its nutrient solution different with dregs of beans and ingredient and contain
Amount, mentions high heat and mass transfer efficiency, output increased.
Claims (9)
1. a kind of produce γ-polyglutamic acid method using lichens bud pole bacterium solid state fermentation, it is characterized in that: by solid state fermentation
It is inoculated with lichens bud pole bacterium after medium sterilization and stands solid state fermentation, produces γ-polyglutamic acid；The solid-state fermentation culture medium
Including solid fermentation base-material and nutritional supplement, the solid fermentation base-material includes dregs of beans and natural porous carrier.
2. a kind of utilization lichens bud pole bacterium solid state fermentation according to claim 1 produces γ-polyglutamic acid method,
It is characterized in that, the natural porous carrier is the natural peral rock or vermiculite that aperture is 3-6mm.
3. a kind of utilization lichens bud pole bacterium solid state fermentation according to claim 1 produces γ-polyglutamic acid method,
It is characterized in that, the nutritional supplement is glucose, sodium glutamate, K2SO4、(NH4)2SO4, anhydrous CaCl2、FeSO4、MgSO4
It is formed with water, their thousand points of concentration of weight respectively are, 60-100 ‰, 40-80 ‰, 10-20 ‰, 5-10 ‰, 0.5-
1 ‰, 0-0.1 ‰, 0-0.5 ‰ remaining be water, pH value be 7.0-7.2.
4. a kind of utilization lichens bud pole bacterium solid state fermentation according to claim 1 produces γ-polyglutamic acid method,
It is characterized in that, the mass ratio of natural porous carrier and dregs of beans is 1-3:7-9 in the solid fermentation base-material, the solid hair
The mass ratio of ferment base-material and nutritional supplement is 1:1.0-1.5.
5. a kind of utilization lichens bud pole bacterium solid state fermentation according to claim 4 produces γ-polyglutamic acid method,
It is characterized in that, the preparation step of the solid-state fermentation culture medium are as follows: vermiculite will be sieved, mix and make after dregs of beans grinding and sieving
For solid fermentation base-material；Nutritional supplement is added in solid fermentation base-material and is uniformly mixed, solid fermentation culture medium is obtained.
6. a kind of utilization lichens bud pole bacterium solid state fermentation according to claim 1 produces γ-polyglutamic acid method,
It is characterized in that, the sterilizing methods of the solid-state fermentation culture medium are solid fermentation culture medium, it is fitted into polyethylene plastic bag, pricks
Tight sack, in 105 DEG C boiling 20 minutes, sterilizing.
7. a kind of utilization lichens bud pole bacterium solid state fermentation according to claim 1 produces γ-polyglutamic acid method,
It is characterized in that, the inoculum concentration of the lichens bud pole bacterium is 5% -10%, after the sterilizing of solid-state fermentation culture medium, it is cooled to
It is inoculated at 50 DEG C.
8. a kind of utilization lichens bud pole bacterium solid state fermentation according to claim 1 produces γ-polyglutamic acid method,
It is characterized in that, the standing solid state fermentation are as follows: after the sterilizing of solid-state fermentation culture medium, by 5-10% when being cooled to 50 DEG C
(V/W) inoculation lichens bud pole bacterium is sufficiently stirred in solid-state fermentation culture medium, strain is made to be uniformly distributed in media surface,
Incubator after inoculation keeps 35-37 DEG C of constant temperature, and humidity is strictly maintained at 50% or more in incubator, ferments 3-6 days.
9. a kind of utilization lichens bud pole bacterium solid state fermentation according to claim 1 produces γ-polyglutamic acid method,
It is characterized in that, stand solid state fermentation after the completion of, extract γ-polyglutamic acid the step of are as follows: be added 5-10 times of solid fermentation object
The water of volume stirs 1-2 hours, extracts the γ-polyglutamic acid of lichens bud pole bacterium secretion in the fermentation medium, passes through
After 100-200 mesh net filtrations, filtrate is collected, filtrate concentrated by rotary evaporation to 20-60g/L of concentration adds 1-4 times of volumes
Ethyl alcohol stands 6-48 hours, precipitating is collected by centrifugation, and then with the ethanol washing of 1-4 times of volumes, vacuum freeze drying is obtained
Refine γ-polyglutamic acid.
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| CN110195086A (en) * | 2019-06-11 | 2019-09-03 | 天津科技大学 | A method of gamma-polyglutamic acid is produced using soy sauce residues solid state fermentation |
| CN112480394A (en) * | 2020-12-01 | 2021-03-12 | 广西大学 | Method for separating and purifying ultra-high molecular weight poly-gamma-glutamic acid from high-viscosity fermentation liquor |
| CN112813114A (en) * | 2021-04-01 | 2021-05-18 | 上海应用技术大学 | Method for producing gamma-polyglutamic acid by solid-state fermentation of soybean curd residue |
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