CN106281659A - Method for extracting protein in a kind of seed of Flos Moutan dregs of rice - Google Patents
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice Download PDFInfo
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- CN106281659A CN106281659A CN201610782586.0A CN201610782586A CN106281659A CN 106281659 A CN106281659 A CN 106281659A CN 201610782586 A CN201610782586 A CN 201610782586A CN 106281659 A CN106281659 A CN 106281659A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
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- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
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- Food Science & Technology (AREA)
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- Medicines Containing Plant Substances (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention provides method for extracting protein in a kind of seed of Flos Moutan dregs of rice, comprise the following steps: the raw material seed of Flos Moutan dregs of rice are pulverized by (1), cross 60 80 mesh sieves, obtain seed of Flos Moutan dregs of rice powder；(2) take seed of Flos Moutan dregs of rice powder, be dissolved in water, be mixed into serosity stand-by；(3) the molten pH value of alkali of serosity described in regulating step (2), carries out aqueous slkali insulation extraction；(4) centrifugal, take supernatant, regulate supernatant pH value；(5) after standing, centrifugal, discard upper water solution, abandoning supernatant, with distilled water by washing of precipitate to neutrality, lyophilization, i.e. obtain seed of Flos Moutan protein.Method for extracting protein in the seed of Flos Moutan dregs of rice of the present invention, in the seed of Flos Moutan dregs of rice, the extraction ratio of protein is high, and have simple to operate, the suitability strong, is suitable for large-scale production, the advantage of low cost.
Description
Technical field
The invention belongs to technical field of food biotechnology, especially relate to method for extracting protein in a kind of seed of Flos Moutan dregs of rice.
Background technology
Paeonia suffruticosa is Mao Moke, Paeonia plant, has the title of " aromatic ".Seed of Flos Moutan rich in fatty acid, protein, 18 kinds
Aminoacid, various trace elements and multivitamin, the peony seed oil developed through seed of Flos Moutan integrates nutrition and health care
Natural nuisance-free is eaten.From peony seed oil since on March 22nd, 2011 obtains new resource food approval, the utilization of Peony Resource becomes
Focus for people's research.
Seed of Paeonia suffruticosa is dry, the ripe seed of ranunculaceae peony, and the annual production of seed of Flos Moutan is higher, but
Relatively low currently with rate ratio, only small part seed of Paeonia suffruticosa is used as seed propagation, does not play its distinctive economic worth.Paeonia suffruticosa
Seed oil content is high, and about 29%, and the mainly unsaturated fatty acid such as linolenic acid, linoleic acid, wherein linolenic acid content reaches
64.14%, linoleic acid content reaches 22.78%, has preferable alimentary health-care function.Additionally, the active substance in seed of Flos Moutan
Also having special efficacy, such as antioxidation, antibacterial, antiinflammatory, pain relieving etc., conventional seed of Paeonia suffruticosa the most among the people cures the diseases such as lumbago and skelalgia
Sick.
The seed of Flos Moutan dregs of rice are the solid waste that seed of Flos Moutan produces after physical squeezing carries oil, seldom have utilization, cause
The great wasting of resources and environmental pollution.In order to find the economic worth of protein, a lot of people is had to put into the seed of Flos Moutan dregs of rice
In the research of component analysis.Yi Junpeng etc. have delivered " chemical constitution study of seed of Flos Moutan " (" research and development of natural products "),
Found by research, in seed of Flos Moutan in addition to saccharide, fat, protein, possibly together with oleanolic acid, hederagenin, Rhizoma Kaempferiae
Phenol, luteolin, apigenin, chrysoeriol, root Portugal plain, cis of trans Portugal root element, trans-resveratrol, cupreol, bean steroid
Alcohol and β-daucosterol isoreactivity material.
In the seed of Flos Moutan dregs of rice, crude protein content reaches 19.03%, has high nutritive value, and has special fragrance
Abnormal smells from the patient.If can be developed as a kind of natural plant protein, the utilization rate of seed of Flos Moutan can not only be improved, and can
Solve by the discarded problem of environmental pollution caused of the seed of Flos Moutan dregs of rice.
Conventional phytoprotein extracting method has: the molten method of (1) dilute salt, this method can weaken the hydrogen bond between protein, albumen
The dissolubility of matter is preferable, but the extraction ratio ratio of protein is relatively low；(2) organic solvent extractionprocess, can destroy protein surface aquation
Layer；(3) alkali extraction-acid precipitation, protein different solubility under different pH condition, have that technique is simple, the suitability strong, extraction ratio
High advantage；(4) enzymatic isolation method, protease cutting protein reduces protein molecular weight, increases hydrophilic group；(5) ultrasound wave is auxiliary
Help extraction, utilize ultrasound wave to accelerate molecular motion and reduce extraction time.
In prior art, the patent of Application No. 201310477491.4 discloses a kind of from seed of Flos Moutan or the seed of Flos Moutan dregs of rice
Extract protein and the method for dietary fiber, use enzymatic isolation method and alkali extraction-acid precipitation to extract albumen from seed of Flos Moutan or the seed of Flos Moutan dregs of rice
Matter and dietary fiber, the method complex process, with organic solvent, the remaining oils and fats in the seed dregs of rice first need to be got rid of, therefore reality should
By the middle problem that there is organic solvent pollution.
Summary of the invention
For above-mentioned technical problem, it is an object of the invention to provide method for extracting protein in a kind of seed of Flos Moutan dregs of rice,
Simplify the step removing oils and fats, decrease the pollution of organic solvent, simplify technological process, and by optimization experiment condition,
Improving the extraction ratio of protein in the seed of Flos Moutan dregs of rice, the further development and utilization for the seed of Flos Moutan dregs of rice provides theoretical foundation.
The technical solution used in the present invention is:
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, comprises the following steps:
(1) the raw material seed of Flos Moutan dregs of rice are pulverized, cross 60-80 mesh sieve, obtain seed of Flos Moutan dregs of rice powder；
(2) take seed of Flos Moutan dregs of rice powder, be dissolved in water, be mixed into serosity stand-by；
(3) the molten pH value of alkali of serosity described in regulating step (2), carries out aqueous slkali insulation extraction；
(4) centrifugal, take supernatant, regulate supernatant pH value, precipitating proteins；
(5) after standing, centrifugal, abandoning supernatant, with distilled water by washing of precipitate to neutral, lyophilization, i.e. obtain male
Red seed protein.
Method for extracting protein in the seed of Flos Moutan dregs of rice of the present invention, wherein, seed of Flos Moutan dregs of rice powder and water in step (2)
Solid-liquid ratio is 1:10～1:40.
Method for extracting protein in the seed of Flos Moutan dregs of rice of the present invention, wherein, in step (3) the molten pH value of alkali be 7.0～
11.0。
Method for extracting protein in the seed of Flos Moutan dregs of rice of the present invention, wherein, in step (3) alkali solubility temperature be 35 DEG C～
65℃。
Method for extracting protein in the seed of Flos Moutan dregs of rice of the present invention, wherein, in step (3), the alkali molten time is 30min
～180min.
Method for extracting protein in the seed of Flos Moutan dregs of rice of the present invention, wherein, in step (4), supernatant pH value is 2.0
～5.0.
Method for extracting protein in the seed of Flos Moutan dregs of rice of the present invention, wherein, in step (2), solid-liquid ratio is 1:35, step
Suddenly in (3), the molten pH value of alkali is 10, and alkali solubility temperature is 55 DEG C, and the alkali molten time is 150min.
The method have the benefit that
Method for extracting protein in the seed of Flos Moutan dregs of rice of the present invention, simplifies the step removing oils and fats, has decreased
The pollution of machine solvent, in the seed of Flos Moutan dregs of rice, the extraction ratio of protein is high, and have simple to operate, the suitability strong, it is extensive raw to be suitable for
Product, the advantage of low cost.
Accompanying drawing explanation
Fig. 1 be the present invention the seed of Flos Moutan dregs of rice in the flow chart of method for extracting protein；
Fig. 2 is that in the present invention, the molten pH value of Different Alkali affects schematic diagram to seed of Flos Moutan cake protein matter dissolution rate；
Fig. 3 be in the present invention different feed liquid comparison seed of Flos Moutan cake protein matter dissolution rate affect schematic diagram；
Fig. 4 is that in the present invention, Different Alkali solubility temperature affects schematic diagram to seed of Flos Moutan cake protein matter dissolution rate；
Fig. 5 be in the present invention Different Alkali molten time seed of Flos Moutan cake protein matter dissolution rate affected schematic diagram；
Fig. 6 is that in the present invention, different pH value affect schematic diagram to seed of Flos Moutan cake protein matter dissolution rate.
Below in conjunction with specific embodiments and the drawings, the invention will be further described.
Detailed description of the invention
The present embodiment seed of Flos Moutan dregs of rice are with Tongling, Anhui Province Ya Tong agro-ecology Science and Technology Ltd. Paeonia suffruticosa after squeezing carries oil
The seed dregs of rice are subjects, and it is as follows that this seed of Flos Moutan dregs of rice basis analyzes method: use GB 5009.3-2010 (105 DEG C of constant weights
Method), GB/T 14489.2-2008 (N takes 5.71) Kjeldahl's method, GB/T 5009.8-2008 Soxhlet extraction method, GB/T
5515-2008 and GB/T 5009.4-2010 measure respectively the moisture of the seed of Flos Moutan dregs of rice, crude protein content, crude fat content,
Total sugar content, crude fiber content and content of ashes, replication 3 times, the basis content analysis result such as table 1 of the seed of Flos Moutan dregs of rice
Shown in, in following example, protein content * in protein (g)/seed of Flos Moutan dregs of rice in dissolution rate (the %)=supernatant of protein
100%.
Embodiment 1
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, comprises the following steps:
(1) the raw material seed of Flos Moutan dregs of rice are pulverized, cross 60-80 mesh sieve, obtain seed of Flos Moutan dregs of rice powder；
(2) take 5g seed of Flos Moutan dregs of rice powder, add 100mL water, i.e. solid-liquid ratio 1:20 (g/mL), be mixed into serosity stand-by；
(3) the molten pH value of alkali with serosity described in 0.5mol/LNaOH regulating step (2) is 10, and alkali solubility temperature 50 DEG C
The lower molten 90min of alkali carries out insulation extraction；
(4) being centrifuged 20min with 3000r/min, take supernatant, adding dilute hydrochloric acid regulation supernatant pH value is 5；
(5) stand after 4h, be centrifuged 20min with 3000r/min, abandoning supernatant, with distilled water by washing of precipitate to neutral,
Lyophilization, i.e. obtains seed of Flos Moutan protein, and replication is averaged for 3 times.
Embodiment 2
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), the molten pH value of alkali is in addition to 7.0, and other is with implementing
Example 1.
Embodiment 3
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), the molten pH value of alkali is in addition to 8.0, and other is with implementing
Example 1.
Embodiment 4
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), the molten pH value of alkali is in addition to 9.0, and other is with implementing
Example 1.
Embodiment 5
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), the molten pH value of alkali is in addition to 9.5, and other is with implementing
Example 1.
Embodiment 6
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), the molten pH value of alkali is in addition to 11.0, and other is with implementing
Example 5.
Embodiment 7
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (2), solid-liquid ratio is in addition to 1:1, other same embodiment
5。
Embodiment 8
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (2), solid-liquid ratio is in addition to 1:15, other same embodiment
5。
Embodiment 9
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (2), solid-liquid ratio is in addition to 1:25, other same embodiment
5。
Embodiment 10
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (2), solid-liquid ratio is in addition to 1:30, other same embodiment
5。
Embodiment 11
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (2), solid-liquid ratio is in addition to 1:35, other same embodiment
5。
Embodiment 12
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (2), solid-liquid ratio is in addition to 1:40, other same embodiment
5。
Embodiment 13
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), alkali solubility temperature is in addition to 35 DEG C, and other is with implementing
Example 5.
Embodiment 14
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), alkali solubility temperature is in addition to 45 DEG C, and other is with implementing
Example 5.
Embodiment 15
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), alkali solubility temperature is in addition to 55 DEG C, and other is with implementing
Example 5.
Embodiment 16
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (4), alkali solubility temperature is in addition to 60 DEG C, and other is with implementing
Example 5.
Embodiment 17
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), alkali solubility temperature is in addition to 65 DEG C, and other is with implementing
Example 5.
Embodiment 18
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), the alkali molten time is in addition to 30min, and other is with real
Execute example 5.
Embodiment 19
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), the alkali molten time is in addition to 60min, and other is with real
Execute example 5.
Embodiment 20
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), the alkali molten time is in addition to 120min, and other is with real
Execute example 5.
Embodiment 21
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), the alkali molten time is in addition to 150min, and other is with real
Execute example 5.
Embodiment 22
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (3), the alkali molten time is in addition to 180min, and other is with real
Execute example 5.
Embodiment 23
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (4), supernatant pH value is in addition to 2.0, and other is with real
Execute example 5.
Embodiment 24
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (4), supernatant pH value is in addition to 3.0, and other is with real
Execute example 5.
Embodiment 25
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (4), supernatant pH value is in addition to 3.5, and other is with real
Execute example 5.
Embodiment 26
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (4), supernatant pH value is in addition to 4.0, and other is with real
Execute example 5.
Embodiment 27
Method for extracting protein in a kind of seed of Flos Moutan dregs of rice, in step (4), supernatant pH value is in addition to 6.0, and other is with real
Execute example 5.
According to the single factor test result of seed of Flos Moutan cake protein matter extraction ratio in above-described embodiment 1-27, by seed of Flos Moutan cake protein matter
Alkali solubility temperature, the molten pH value of alkali, alkali molten time and solid-liquid ratio as index, carry out four factor three horizontal quadratures test, orthogonal reality
The factor design tested is shown in Table 6.
Table 1 seed of Flos Moutan dregs of rice basis analysis result
The molten pH value of table 2 Different Alkali affects result to seed of Flos Moutan cake protein matter dissolution
In embodiment 1-6, solid-liquid ratio (m:v) is 1:20, alkali solubility temperature 50 DEG C, alkali molten time 90min, the molten pH value of Different Alkali
Be 7.0,8.0,9.0,9.5,10.0 and 11.0 on the impact of seed of Flos Moutan cake protein matter dissolution rate as shown in table 2 and Fig. 2, result table
Bright selection pH value is 9.5 for the suitableeest.
The impact of table 3 different feed liquid comparison seed of Flos Moutan cake protein matter dissolution
In embodiment 5 and embodiment 7-12, the molten pH value of alkali is 9.5, alkali solubility temperature 50 DEG C, alkali molten time 90min, different material
Liquor ratio (m:v) 1:10,1:15,1:20,1:25,1:30,1:35 and 1:40 affect result such as to seed of Flos Moutan cake protein matter dissolution
Shown in table 3 and Fig. 3, result shows that selecting 1:30 is the suitableeest solid-liquid ratio.
The impact on seed of Flos Moutan cake protein matter dissolution of the table 4 Different Alkali solubility temperature
In embodiment 5 and embodiment 13-17, the molten pH value of alkali is 9.5, and solid-liquid ratio (m:v) is 1:20, alkali molten time 90min,
Different Alkali solubility temperature 35 DEG C, 45 DEG C, 50 DEG C, 55 DEG C, 60 DEG C and the 65 DEG C impact such as tables 4 on seed of Flos Moutan cake protein matter dissolution and figure
Shown in 4, result shows to select 50 DEG C for the suitableeest alkali solubility temperature.
The impact on seed of Flos Moutan cake protein matter dissolution of the table 5 Different Alkali molten time
In embodiment 5 and embodiment 18-22, the molten pH value of alkali is 9.5, and solid-liquid ratio (m:v) is 1:20, alkali solubility temperature 50 DEG C,
Different Alkali molten time 30min, 60min, 90min, 120min, 150min and 180min impact on seed of Flos Moutan cake protein matter dissolution
Result is as shown in table 5 and Fig. 5, and selecting 120min is the suitableeest alkali molten time.
In embodiment 5 and embodiment 23-27, in step (4), supernatant pH value is 2.0,3.0,3.5,4.0,5.0 and 6.0
Affecting result as shown in Figure 6 to what seed of Flos Moutan cake protein matter precipitated, result shows, when pH value is 3.5, seed of Flos Moutan cake protein matter is sunk
Shallow lake rate reaches maximum 97.46%, and therefore selecting pH value is 3.5 to be optimum pH.
Table 6 orthogonal test factor table
| Level | The A/ time (min) | B/ temperature (DEG C) | C/pH value | D/ solid-liquid ratio (m:v) |
| 1 | 90 | 45 | 9.0 | 1:25 |
| 2 | 120 | 50 | 9.5 | 1:30 |
| 3 | 150 | 55 | 10.0 | 1:35 |
As table 6 understands, draw according to orthogonal experiment: pH value solid-liquid ratio > temperature > time, the suitableeest process combination is: pH
Value is 10, and solid-liquid ratio is 1:35, and alkali solubility temperature is 55 DEG C, and the alkali molten time is 150min.
The optimum process condition combination drawn according to orthogonal experiment, amplifies test, observes and amplify for protein dissolution
The impact of rate, in the seed of Flos Moutan dregs of rice, the dissolution rate of protein is respectively 89.2%, 89.3% and 88.9%, and meansigma methods is 89.1%,
Therefore, it can show that this prioritization scheme feasibility is higher.
Embodiment described above is only to be described the preferred embodiment of the present invention, the not model to the present invention
Enclose and be defined, on the premise of designing spirit without departing from the present invention, the those of ordinary skill in the art technical side to the present invention
Various deformation that case is made and improvement, all should fall in the protection domain that claims of the present invention determines.
Claims (7)
1. method for extracting protein in seed of Flos Moutan dregs of rice, it is characterised in that: comprise the following steps:
(1) the raw material seed of Flos Moutan dregs of rice are pulverized, cross 60-80 mesh sieve, obtain seed of Flos Moutan dregs of rice powder；
(2) take seed of Flos Moutan dregs of rice powder, be dissolved in water, be mixed into serosity stand-by；
(3) the molten pH value of alkali of serosity described in regulating step (2), carries out aqueous slkali insulation extraction；
(4) centrifugal, take supernatant, regulate supernatant pH value, precipitating proteins；
(5) after standing, centrifugal, abandoning supernatant, with distilled water by washing of precipitate to neutral, lyophilization, i.e. obtain seed of Flos Moutan
Protein.
Method for extracting protein in the seed of Flos Moutan dregs of rice the most according to claim 1, it is characterised in that: Paeonia suffruticosa in step (2)
The solid-liquid ratio of seed dregs of rice powder and water is 1:10～1:40.
Method for extracting protein in the seed of Flos Moutan dregs of rice the most according to claim 1, it is characterised in that: in step (3), alkali is molten
PH value is 7.0～11.0.
Method for extracting protein in the seed of Flos Moutan dregs of rice the most according to claim 1, it is characterised in that: in step (3), alkali is molten
Temperature is 35 DEG C～65 DEG C.
Method for extracting protein in the seed of Flos Moutan dregs of rice the most according to claim 1, it is characterised in that: in step (3), alkali is molten
Time is 30min～180min.
6. according to method for extracting protein in the seed of Flos Moutan dregs of rice described in claim 1-5 any one, it is characterised in that: step
(4) in, supernatant pH value is 2.0～5.0.
7. according to method for extracting protein in the seed of Flos Moutan dregs of rice described in claim 1 any one, it is characterised in that: step
(2) in, solid-liquid ratio is 1:35, and in step (3), the molten pH value of alkali is 10, and alkali solubility temperature is 55 DEG C, and the alkali molten time is 150min.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108887461A (en) * | 2018-05-25 | 2018-11-27 | 常州大学 | Peony seeds albumen and its extracting method |
| CN108998491A (en) * | 2018-08-29 | 2018-12-14 | 西南大学 | A method of extracting protein in the peony seeds dregs of rice |
| CN109207550A (en) * | 2018-11-22 | 2019-01-15 | 哈尔滨氧态健康科技股份有限公司 | A kind of preparation method of peony seeds polypeptide |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104322857A (en) * | 2014-10-14 | 2015-02-04 | 江苏科技大学 | Modified peony seed meal protein preparation method |
-
2016
- 2016-08-30 CN CN201610782586.0A patent/CN106281659A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104322857A (en) * | 2014-10-14 | 2015-02-04 | 江苏科技大学 | Modified peony seed meal protein preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 李加兴: "食品与机械", 《食品与机械》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108887461A (en) * | 2018-05-25 | 2018-11-27 | 常州大学 | Peony seeds albumen and its extracting method |
| CN108998491A (en) * | 2018-08-29 | 2018-12-14 | 西南大学 | A method of extracting protein in the peony seeds dregs of rice |
| CN109207550A (en) * | 2018-11-22 | 2019-01-15 | 哈尔滨氧态健康科技股份有限公司 | A kind of preparation method of peony seeds polypeptide |
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Application publication date: 20170104 |