CN102640935A - Method for extracting dietary fiber in banana skin by adopting enzymic method - Google Patents

Method for extracting dietary fiber in banana skin by adopting enzymic method Download PDF

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Publication number
CN102640935A
CN102640935A CN2012101628413A CN201210162841A CN102640935A CN 102640935 A CN102640935 A CN 102640935A CN 2012101628413 A CN2012101628413 A CN 2012101628413A CN 201210162841 A CN201210162841 A CN 201210162841A CN 102640935 A CN102640935 A CN 102640935A
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China
Prior art keywords
dietary fiber
enzymolysis
banana skin
alcohol
washing
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Pending
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CN2012101628413A
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Chinese (zh)
Inventor
王白鸥
葛邦国
马超
崔春红
朱风涛
和法涛
赵岩
初乐
吕绪强
马寅斐
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JINAN FRUITS RESEARCH INSTITUTE ALL CHINA FEDERATION OF SUPPLY AND MARKETING COOPERATIVES
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JINAN FRUITS RESEARCH INSTITUTE ALL CHINA FEDERATION OF SUPPLY AND MARKETING COOPERATIVES
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Priority to CN2012101628413A priority Critical patent/CN102640935A/en
Publication of CN102640935A publication Critical patent/CN102640935A/en
Pending legal-status Critical Current

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Abstract

The invention belongs to the field of agricultural product processing, and discloses a method for extracting dietary fiber in banana skin by adopting an enzymic method. The method comprises the operation steps of pretreatment, smashing, enzymolysis, ethonal precipitation, filtrate collection and drying. The extraction of the current dietary fiber generally comprises a chemical method, a fermentation method, a biological enzyme treatment method and the like; because strong acid, strong alkali and high-temperature treatment are adopted for the chemical method, the corrosion to an extracting container, a pipeline and a material pump are quite serious, the acid degree is required to be regulated repeatedly in the extraction process, a great amount of cation and anion are brought in, and the prepared dietary fiber is low in content and poor in color; the dietary fiber prepared by adopting the fermentation method has high content of effective components, is less in pollution, has a long production cycle, is high in cost, and is not beneficial to scale production; and because the catalysis ratio of enzyme is high, the action condition is mild, the enzymic method technology does not require high-temperature high-pressure condition, the equipment requirement is simple, and the effective components have less damage.

Description

The method of dietary fiber in a kind of Enzymatic Extraction banana skin
Technical field:
The invention belongs to the processing of farm products field, the method for dietary fiber in a kind of Enzymatic Extraction banana skin.
Background technology:
(Dietary Fiber DF) is meant the summation of edibility plant cell, polysaccharide, lignin and the related substances that can not be digested and assimilated by the human body endogenous enzymes to dietary fiber, is called as mankind's " the seventh-largest nutrient ".Its character can be divided into water-soluble dietary fiber (SDF) and water insoluble dietary fiber (IDF) by dissolubility.
Modern study shows: dietary fiber has different physiological roles; Keeping normal blood sugar, blood fat and protein level; The control body weight; Prevention diabetes, coronary heart disease suppress body to absorption of cholesterol and the drainage that increases cholic acid, and preventing and treating aspects such as intestinal cancer and other intestines problems has significant effect.Multi-form dietary fiber with the source has different physiological functions; The function that water insoluble dietary fiber has mainly is to act on enteron aisle; Make the wriggling effect that the excreta retentiveness improves, volume increases, generation is mechanical in the enteron aisle, improve the constipation of human body etc.; The multiple metabolism of human body can participated in or influence to water soluble ingredient, like fat metabolism, carbohydrate metabolism etc.
Banana skin is one of main accessory substance of banana processing enterprise, and tare weight accounts for about 35% of fruit weight.The main component of banana skin is a dietary fiber, also contains more than ten kind of inorganic elements such as crude protein, fat, carbohydrate and potassium, calcium, iron in addition.Yet the industrial utilization to banana skin does not appear in the newspapers as yet, and is very little to the application study of banana skin yet, only limits to extract pectin and is used as the chicken feed, and a large amount of banana skins is used as rubbish and abandons, and causes environmental pollution, also is the waste of resource simultaneously.
Summary of the invention:
The present invention is to be raw material with the banana skin, adopts enzyme process from banana skin, to extract dietary fiber.
(1) the banana skin preliminary treatment is boiled 3min to banana skin with hot water.
(2) pulverize a banana skin that boiled and be cut into segment, pulverize with pulverizer.
(3) AMS constant temperature enzymolysis adds a certain amount of AMS, stirs, and places water bath with thermostatic control to be hydrolyzed while stirring.
(4) the papain enzymolysis water bath with thermostatic control cools to the papain enzymolysis temperature, adds a certain amount of papain, stir, and hydrolysis while stirring.
(5) banana skin behind the enzymolysis is filtered in filtration.
(6) IDF prepares the gained filter residue and uses washing with alcohol, and the filter residue after the washing is water insoluble dietary fiber IDF, and is dry with the electric heating constant temperature air dry oven, obtains the IDF product.
(7) SDF preparation filtrating use precipitation with alcohol 1h, filter then, the gained filter residue with 95% washing with alcohol after, with electric heating constant temperature air dry oven drying, obtain the SDF product.
(8) ethanol reclaims to filtrate and distills recovery ethanol.
Advantage of the present invention:
The extraction of current dietary fiber generally has chemical method; Fermentation method, biology enzyme facture etc. are because chemical method adopts strong acid, highly basic, high-temperature process, quite serious to the corrosion of extraction vessel, pipeline, product pump; In leaching process, need adjust acidity repeatedly; Bring a large amount of zwitterions into, the dietary fiber content of producing is lower, shade deviation; The dietary fiber active constituent content that fermentation method is produced is high, and is of reduced contamination, but the production cycle is long, and cost is high, is unfavorable for the big production of scale; Enzyme method technique is because the catalytic rate of enzyme is high, action condition is gentle, generally do not need high-temperature and high-pressure conditions, so equipment requirements is simple, active ingredient is destroyed few.
The specific embodiment
Extract instance below in conjunction with the banana skin dietary fiber the present invention is done explanation at length, but be not limited only to this.Method described in the embodiment if no special instructions all by conventional method.
(1) the banana skin preliminary treatment is boiled 3min to banana skin with hot water.
(2) pulverize a banana skin that boiled and be cut into segment, pulverize with pulverizer.
(3) AMS constant temperature enzymolysis hydrolysis temperature is that 70 ℃, enzymolysis time 40min, addition are 0.3%.
(4) the papain enzymolysis hydrolysis temperature is that 45 ℃, enzymolysis time are that 40min, addition are 0.2%.
(5) banana skin behind the enzymolysis is filtered in filtration.
(6) IDF prepares the gained filter residue and uses washing with alcohol, and the filter residue after the washing is water insoluble dietary fiber IDF, and is dry with the electric heating constant temperature air dry oven, obtains the IDF product.
(7) SDF preparation filtrating use precipitation with alcohol 1h, filter then, the gained filter residue with 95% washing with alcohol after, with electric heating constant temperature air dry oven drying, obtain the SDF product.
(8) ethanol reclaims to filtrate and distills recovery ethanol.Enzyme solution extracts the Quality Detection of banana skin dietary fiber
The banana skin dietary fiber quality result who adopts the inventive method to make is as shown in table 1 below.
Table 1
Project Dietary fiber content (%, wet basis) SDF∶IDF
The banana skin dietary fiber 9.2 1∶4

Claims (5)

1. the method for dietary fiber in the Enzymatic Extraction banana skin, this method step is following:
(1) the banana skin preliminary treatment is boiled 3min to banana skin with hot water.
(2) pulverize a banana skin that boiled and be cut into segment, pulverize with pulverizer.
(3) AMS constant temperature enzymolysis adds a certain amount of AMS, stirs, and places water bath with thermostatic control to be hydrolyzed while stirring.
(4) the papain enzymolysis water bath with thermostatic control cools to the papain enzymolysis temperature, adds a certain amount of papain, stir, and hydrolysis while stirring.
(5) banana skin behind the enzymolysis is filtered in filtration.
(6) IDF prepares the gained filter residue and uses washing with alcohol, and the filter residue after the washing is water insoluble dietary fiber IDF, and is dry with the electric heating constant temperature air dry oven, obtains the IDF product.
(7) SDF preparation filtrating use precipitation with alcohol 1h, filter then, the gained filter residue with 95% washing with alcohol after, with electric heating constant temperature air dry oven drying, obtain the SDF product.
(8) ethanol reclaims to filtrate and distills recovery ethanol.
2. enzymolysis method of work according to claim 1 is characterized in that: wherein step (3) hydrolysis temperature is that 70 ℃, enzymolysis time 40min, addition are 0.3%.
3. enzymolysis method of work according to claim 1 is characterized in that: wherein step (4) hydrolysis temperature is that 45 ℃, enzymolysis time are that 40min, addition are 0.2%.
4. IDF preparation work method according to claim 1 is characterized in that: wherein step (6) gained filter residue is used washing with alcohol, and the filter residue after the washing is water insoluble dietary fiber IDF, and is dry with the electric heating constant temperature air dry oven.
5. SDF preparation work method according to claim 1 is characterized in that: wherein step (7) filtrating is used precipitation with alcohol 1h, filters then, and the gained filter residue is used 95% washing with alcohol.
CN2012101628413A 2012-05-24 2012-05-24 Method for extracting dietary fiber in banana skin by adopting enzymic method Pending CN102640935A (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103039880A (en) * 2012-12-29 2013-04-17 黄家芬 Method for preparing multi-functional dietary fiber additive by using banana skins
CN103859422A (en) * 2014-03-18 2014-06-18 中国热带农业科学院海口实验站 Production method of banana flower soluble dietary fiber
CN104939092A (en) * 2015-05-30 2015-09-30 福建万亿店中店电子商务有限责任公司 Composite pericarp extract product having heat clearing and lung moistening efficacy
CN105011291A (en) * 2015-07-28 2015-11-04 渠县金穗农业科技有限公司 Radix paeoniae alba-red date-lemon dietary fiber functional beverage
CN105495314A (en) * 2015-12-18 2016-04-20 湖北工业大学 Porous coarse cereal flour including dietary fibers
CN106174461A (en) * 2016-04-17 2016-12-07 马鞍山市志诚科技有限公司 A kind of nutrient formulation nano-particle preventing and treating Sheehan syndrome and preparation processing method
CN107019227A (en) * 2017-04-27 2017-08-08 河南科技大学 A kind of Banana Peel Dietary Fiber, low sugar yoghurt and preparation method thereof
CN107722300A (en) * 2017-09-30 2018-02-23 铜陵家宴生态农业发展有限公司 A kind of hydrogen peroxide is modified the preparation method of banana tegument extract composite aquogel

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CN1911333A (en) * 2006-08-24 2007-02-14 中国药科大学制药有限公司 Process for extraction of peel of banana
CN101715910A (en) * 2009-12-02 2010-06-02 华南农业大学 Composition of banana oligosaccharide and dietary fibre, preparation method and application thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1911333A (en) * 2006-08-24 2007-02-14 中国药科大学制药有限公司 Process for extraction of peel of banana
CN101715910A (en) * 2009-12-02 2010-06-02 华南农业大学 Composition of banana oligosaccharide and dietary fibre, preparation method and application thereof

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103039880A (en) * 2012-12-29 2013-04-17 黄家芬 Method for preparing multi-functional dietary fiber additive by using banana skins
CN103859422A (en) * 2014-03-18 2014-06-18 中国热带农业科学院海口实验站 Production method of banana flower soluble dietary fiber
CN104939092A (en) * 2015-05-30 2015-09-30 福建万亿店中店电子商务有限责任公司 Composite pericarp extract product having heat clearing and lung moistening efficacy
CN105011291A (en) * 2015-07-28 2015-11-04 渠县金穗农业科技有限公司 Radix paeoniae alba-red date-lemon dietary fiber functional beverage
CN105495314A (en) * 2015-12-18 2016-04-20 湖北工业大学 Porous coarse cereal flour including dietary fibers
CN105495314B (en) * 2015-12-18 2019-09-24 湖北工业大学 A kind of porous coarse cereal powder of dietary fiber
CN106174461A (en) * 2016-04-17 2016-12-07 马鞍山市志诚科技有限公司 A kind of nutrient formulation nano-particle preventing and treating Sheehan syndrome and preparation processing method
CN107019227A (en) * 2017-04-27 2017-08-08 河南科技大学 A kind of Banana Peel Dietary Fiber, low sugar yoghurt and preparation method thereof
CN107722300A (en) * 2017-09-30 2018-02-23 铜陵家宴生态农业发展有限公司 A kind of hydrogen peroxide is modified the preparation method of banana tegument extract composite aquogel

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Application publication date: 20120822