CN101715910A - Composition of banana oligosaccharide and dietary fibre, preparation method and application thereof - Google Patents

Composition of banana oligosaccharide and dietary fibre, preparation method and application thereof Download PDF

Info

Publication number
CN101715910A
CN101715910A CN200910194303A CN200910194303A CN101715910A CN 101715910 A CN101715910 A CN 101715910A CN 200910194303 A CN200910194303 A CN 200910194303A CN 200910194303 A CN200910194303 A CN 200910194303A CN 101715910 A CN101715910 A CN 101715910A
Authority
CN
China
Prior art keywords
banana
dietary fiber
drying
enzymolysis
pomace
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN200910194303A
Other languages
Chinese (zh)
Other versions
CN101715910B (en
Inventor
杨公明
王娟
程燕锋
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
South China Agricultural University
Original Assignee
South China Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by South China Agricultural University filed Critical South China Agricultural University
Priority to CN2009101943030A priority Critical patent/CN101715910B/en
Publication of CN101715910A publication Critical patent/CN101715910A/en
Application granted granted Critical
Publication of CN101715910B publication Critical patent/CN101715910B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention discloses a composition of banana oligosaccharide and dietary fibre, a preparation method and an application thereof. The composition comprises banana oligosaccharide, soluble dietary fibre and insoluble dietary fibre, wherein the proportion of the banana oligosaccharide to the soluble dietary fibre to the insoluble dietary fibre is 2.5:1:2 or 2:2:2.In the invention, the banana oligosaccharide is obtained by pulping the fruit flesh of bananas, leaching by water, precipitating by ethanol and freeze-drying in vacuum; the soluble and insoluble dietary fibre of the bananas is obtained by carrying out zymohydrolysis on the fruit residues of the bananas. The composition has the effect of relaxing bowels of a common purge, is mild, does not have stimulation property, overcomes side effects caused by common rhubarb and anthraquinone class purges, can exert the physiological function of preventing colonic and rectal cancers, cardiovascular diseases and the like of the dietary fibre and achieves the aims of retaining water, lubricating and keeping high penetrability; because the composition is natural, safe and effective, the composition is especially suitable for relieving the constipation symptoms of dry, dense and clotty stool and difficult defecation of infants because of eating powdered milk.

Description

Banana oligosaccharide and diet fiber composition and its production and application
Technical field
The invention belongs to food processing technology field, relate to a kind of functional food and preparation method thereof, be specifically related to a kind of banana oligosaccharide and diet fiber composition, its preparation method and application.
Background technology
Constipation is meant the ill state of defecation, comprises that the ight soil drying is discharged smooth and ight soil is not done two kinds of situations of also difficult discharge, refers generally to weekly defecation and is less than 2 ~ 3 times.Can be divided into organic constipation and functional consitipation according to the cause of disease, the former is caused by enteron aisle and systemic disease; The latter refers to that said clinically constipation mainly refers to functional consitipation because defecation reflex is not normal or block the colonic constipation that the proctogenous constipation cause and colonic peristalsis slowly cause.Rheum officinale, anthraquinone class cathartic are usually used in the treatment constipation, but this type of cathartic has side effect, can make the patient that cathartic is produced dependence, the long-term use diminishes colon transfer function and enteron aisle nerve, can cause more serious functional, intractable constipation on the contrary, produce the black change of colon, cause the purgatives colon.
Domestic the treatment constipation is mainly concentrated on zoopery and the clinical effectiveness and the mechanism research aspect of Chinese medicine compound prescription, the research of defaecation food rarely has report.
According to biochemistry noun validation board nomenclature, oligosaccharides is defined as comprising the polymer of 3~10 monose.To be monose couple together the low polymerization sugar with straight chain or branched chain that forms by glycosidic bond to oligosaccharides, therefore claims again " compound sugar ".Some can not be had following physiological function by the oligosaccharides that human consumption absorbs: the propagation of beneficial bacteria such as Bifidobacterium, Bacillus acidi lactici in (1) promotion human body, thus the growth of spoilage organisms in the inhibition enteron aisle improves the intestinal microecology environment; (2) in colon, produced SCFA,, reduce the pH value of enteron aisle as acetate, propionic acid, butyric acid etc. by fermentation; (3) improve immunity of organisms, reduce the concentration of poisonous tunning such as amine, phenol, the prevention colorectal cancer; (4) anti-treating constipation removed toxin; (5) increase the absorption that some must mineral element.
Dietary fiber mainly comprises cellulose, hemicellulose, pectin and hydrophilic colloid material, lignin and the material that is not decomposed by the human consumption enzyme.Different dietary fibers can be divided into soluble dietary fiber and insoluble diedairy fiber two big classes by dissolution characteristics, and the two merging is called total dietary fiber.Soluble dietary fiber is meant not by the human body alimentary canal enzymic digestion, but dissolving in warm water or hot water and its aqueous solution again can be by 4 times of reppd that part of dietary fiber of volume ethanol, it mainly refers to stored substance and secretion in the plant cell, also comprises part microbial polysaccharide and synthetic class polysaccharide such as pectin, melon bean gum, alginate, glucan and fungi polysaccharide; The insolubility dietary fiber is meant not by human body alimentary canal enzymic digestion and water-fast dietary fiber, comprises cellulose, hemicellulose, lignin etc.Dietary fiber has anti-treating constipation and hemorrhoid, colorectal cancer, angiocardiopathy, and the diabetes-alleviating symptom is regulated the enteron aisle effect and alleviated physiological function such as obesity.
How obtaining natural, effective oligosaccharides and dietary fiber is the focus of studying at present, but compound sugar defaecation Research on effect is mainly concentrated on FOS and application study in Animal nutrition thereof both at home and abroad, and it is used as feed addictive.Research to dietary fiber mainly contains extraction preparation from pomace, orange peel, bean dregs, the root of kudzu vine, marine alga etc., and the report of specializing in the edible fiber cathartic function is few.
Banana is the tropical and subtropical zone characteristic fruit, is the fourth-largest fruit after output is only second to apple, oranges and tangerines, pears in China, and it is nutritious, smell delicate fragrance fragrance is distinguished the flavor of sweet and tasty mouthful, and meat is soft lubricious, old children is all suitable, and banana promotes enterogastric peristalsis, and defecation, cosmetology function common people generally acknowledge.Chinese scholars is discovered the greater functionality of banana in recent years, as reducing blood lipid, hypotensive, anti-oxidant, improve immunity and alleviate anxiety etc.
Banana is cited as " king of new fruit " in world's fruits and vegetables conference in 2005.The world all is rapid ascendant trend with the banana total output of China, and China's banana gross output had reached 6,900,000 tons in 2006.Banana is to breathe modification fruit more, and storage tolerance is not perishable, and it is very serious to adopt the back loss, and process technology falls behind simultaneously, promptly wastes and contaminated environment.
Meanwhile, the clear and definite material base of banana treatment constipation does not appear in the newspapers, and the mechanism of action it be unclear that, and does not see the research report with banana oligosaccharide and dietary fiber combined preparation bowel relaxing functions food.
Summary of the invention
An object of the present invention is to overcome the deficiencies in the prior art, a kind of banana oligosaccharide and diet fiber composition are provided.
Another object of the present invention provides described preparation of compositions method.
A further object of the invention provides the application of described composition.
Purpose of the present invention is achieved by the following technical programs:
A kind of banana oligosaccharide and diet fiber composition are provided, comprise banana oligosaccharide, soluble dietary fiber and insolubility dietary fiber, the mass ratio of described banana oligosaccharide, soluble dietary fiber and insolubility dietary fiber is 2.5: 1: 2 or 2: 2: 2.
The present invention provides the preparation method of above-mentioned banana oligosaccharide and diet fiber composition simultaneously, may further comprise the steps:
(1) banana peeling, with the pulp making beating, the pulp after the flooding making beating, pomace is put in addition, absolute ethyl alcohol precipitation extract, centrifugation, collecting precipitation, the vacuum freeze-drying precipitation prepares banana oligosaccharide;
(2) add enzyme in the pomace of putting in addition after step (1) lixiviate and carry out enzymolysis, centrifugation behind the enzymolysis is isolated the precipitation vacuum freeze-drying and is got banana insolubility dietary fiber; Supernatant precipitates through absolute ethyl alcohol, vacuum freeze-drying precipitate soluble dietary fiber;
(3) proportionally mix banana oligosaccharide, soluble dietary fiber and insolubility dietary fiber and get described composition.
The described making beating of above-mentioned steps (1) can be adopted common fruit and vegetable pulping machine, and beating time is 2~4min; The feed liquid w/v of described flooding is 1: 1, extraction temperature is 50~60 ℃, extraction time is 50~60min, extract 4 times, merge No. 4 times extract, with the absolute ethyl alcohol precipitation extract of 4 times of extracting liquid volumes, described centrifugation is with the centrifugal 12~14min of 3500~4500 rev/mins rotating speed, and described vacuum freeze-drying is that freeze-drying 4~5h gets banana oligosaccharide under 50Pa~150Pa;
The described enzymolysis of above-mentioned steps (2) comprises two steps:
Steps A: in pomace, add amylase, diastatic consumption is 3.6~4.0% of a pomace weight, 95~100 ℃ of operative temperatures, adding calcium chloride adjusting calcium ion concentration is 15~20mmol/L, regulating the pH value with hydrochloric acid or sodium hydroxide solution is 4~4.5,95~100 ℃ of water-bath vibration enzymolysis 8~10h.
Step B: add protease in the mixture behind the steps A enzymolysis, the consumption of protease is 0.20~0.25% of a pomace weight, and 50~55 ℃ of operative temperatures, pH value are 7~7.5, water-bath vibration enzymolysis 2~2.5h.
Centrifugal 12~the 14min of rotating speed that the rotating speed of the described centrifugation of step (2) and time are respectively 3500~4500 rev/mins; The consumption of described absolute ethyl alcohol is 4 times of supernatant volumes; Described vacuum freeze-drying is freeze-drying 6~7h under 80Pa~200Pa condition.
One of preferably preparation scheme of the present invention is that banana is removed the peel, banana pulp making beating 2.5min, flooding, feed liquid w/v are 1g: 1ml, and extraction temperature is 60 ℃, extraction time is 50min, extract 4 times, merge No. 4 extracts (pomace is put in addition), concentrate through suction filtration and vacuum, with the absolute ethyl alcohol of 4 times of extracting liquid volumes precipitation, 3500 rev/mins of centrifugal 14min, collecting precipitation, 50Pa~80Pa vacuum freeze-drying 4h obtains the banana oligosaccharide product;
Residual pomace is used for enzymolysis and prepares the solvable and soluble dietary fiber of banana after the lixiviate banana liquid glucose, and wherein the amylase consumption is 3.6% of a pomace weight, 100 ℃ of operative temperatures, and calcium ion concentration 15mmol/L, the pH value is 4,95 ℃ of water-bath vibration enzymolysis 10h; The protease consumption is 0.2% of a pomace weight, and the pH value is 7,50 ℃ of water-bath vibration enzymolysis 2.5h, water-bath vibration enzymolysis 2.5h, centrifugation 14min under 3500 rev/mins of rotating speeds behind the enzymolysis,, the isolated 100Pa of being deposited in~200Pa vacuum freeze-drying 7h gets banana insolubility dietary fiber; Supernatant adds 4 times of volume absolute ethyl alcohols and must precipitate, and is deposited in 100Pa~200Pa vacuum freeze-drying 7h and gets banana soluble dietary fiber finished product;
Compound sugar product and solvable, insolubility dietary fiber finished product are to mix at 2.5: 1: 2 to obtain described composition according to mass ratio.
It is with banana peeling that the present invention preferably prepares two of scheme, with the banana pulp 3min that pulls an oar, and flooding, the feed liquid w/v is 1g: 1ml, and extraction temperature is 50 ℃, and extraction time is 60min, extracts 4 times; Merge No. 4 extracts (pomace is put in addition), concentrate through suction filtration and vacuum, with the absolute ethyl alcohol of 4 times of extracting liquid volumes precipitate, 4500 rev/mins of centrifugal 12min, collecting precipitation, 60Pa~100Pa vacuum freeze-drying 5h obtains the banana oligosaccharide product;
Residual pomace is used for enzymolysis and prepares the solvable and soluble dietary fiber of banana after the lixiviate banana liquid glucose, wherein the amylase consumption is 4.0% of a pomace weight, 95 ℃ of operative temperatures, calcium ion concentration 20mmol/L, the pH value is 4.5,95~100 ℃ of water-bath vibration enzymolysis 8h; The protease consumption is 0.25% of a pomace weight, 55 ℃ of temperature, pH value are 7.5,55 ℃ of water-bath vibration enzymolysis 2h, centrifugation 12min under 4500 rev/mins of rotating speeds behind the enzymolysis, freeze-drying 6h gets banana insolubility dietary fiber under the isolated 80Pa of being deposited in~150Pa vacuum; Adding 4 times of volume absolute ethyl alcohols in the supernatant must precipitate, and is deposited in that freeze-drying 6h gets banana soluble dietary fiber finished product under 80Pa~150Pa vacuum;
Compound sugar product and solvable, insolubility dietary fiber finished product are to mix at 2: 2: 2 to obtain described composition according to portfolio ratio.
Composition of the present invention can be applicable to prepare bowel relaxing functions food, beverage or is used to prepare the medicine for the treatment of constipation.
The invention has the beneficial effects as follows:
(1) the present invention is the defaecation effect that the banana oligosaccharide that obtains of feedstock production and soluble dietary fiber, insolubility diet fiber composition not only possess common cathartic with the banana, and gentle, do not stimulate, common rheum officinale, the caused side effect of anthraquinone class cathartic have been overcome, and can bring into play prevention colorectal cancer, the angiocardiopathy of dietary fiber, the diabetes-alleviating symptom is regulated physiological functions such as enteron aisle effect and fat-reducing.Because dietary fiber of the present invention has stronger retention ability, in conjunction with waterpower, can promote moisture to shift to enteron aisle and intestinal contents, make ight soil have bigger volume and higher wettability, reach the purpose that water holding is lubricated, keep height to ooze, dietary fiber of the present invention is from banana, thing experimental result combined according to the invention, described composition is safe and effective, especially is fit to alleviate the infant because the constipation symptom that the close knot of being hard and dry that edible milk powder causes is difficult to resolve.
(2) the present invention provides technical support for the clear and definite material base and the mechanism of action of research banana treatment constipation, and can effectively solve the pressure of banana fresh-keeping, processing.
(3) the present invention all effectively utilizes banana pulp, kept the nutritional labeling of banana, and the preparation method is simple, and preparation process is simple and easy, and cost is lower.
The specific embodiment
Further describe the present invention below in conjunction with specific embodiment.
Embodiment 1 prepares composition according to following method
(1) banana peeling; Pulp making beating 2.5min, flooding, the feed liquid w/v is 1g: 1ml, and temperature is 60 ℃, and extraction time is 50min, extracts 4 times; Merge No. 4 times extract, pomace is put in addition, and extract concentrates through suction filtration and vacuum, with the absolute ethyl alcohol of 4 times of extracting liquid volumes precipitate, 3500 rev/mins of centrifugal 14min, collecting precipitation, 50Pa~80Pa vacuum freeze-drying 4h obtains the banana oligosaccharide product;
(2) residual pomace is used for enzymolysis and prepares the solvable and soluble dietary fiber of banana after the lixiviate banana liquid glucose:
Steps A: in pomace, add amylase, diastatic consumption is 3.6% of a pomace weight, 100 ℃ of operative temperatures, and it is 15mmol/L that adding calcium chloride makes calcium ion concentration, by adding hydrochloric acid and sodium hydroxide solution adjusting pH value is 4,95~100 ℃ of water-baths vibration enzymolysis 10h;
Step B: add protease in the steps A enzymolysis liquid, the consumption of protease is 0.2% of a pomace weight, 50 ℃ of temperature, and adding hydrochloric acid and sodium hydroxide solution adjusting pH value is 7,50 ℃ of water-baths vibration enzymolysis 2.5h;
At the centrifugal 12~14min of 3500~4500 rev/mins rotating speed, freeze-drying 7h gets banana insolubility dietary fiber under the isolated 100Pa of being deposited in~200Pa vacuum behind the enzymolysis; The absolute ethyl alcohol that adds 4 times of supernatant volumes in the supernatant must precipitate, and is deposited in that freeze-drying 7h gets banana soluble dietary fiber finished product under 100Pa~200Pa vacuum;
(3) compound sugar product and solvable, insolubility dietary fiber finished product are to mix at 2.5: 1: 2 to obtain described composition 1 according to mass ratio.
Embodiment 2
(1) banana peeling; Pulp making beating 3min, flooding, the feed liquid w/v is 1g: 1ml, and temperature is 50 ℃, and extraction time is 60min, extracts 4 times; Merge No. 4 times extract, pomace is put in addition, extract through conventional suction filtration and vacuum concentrate the back with the absolute ethyl alcohol of 4 times of extracting liquid volumes precipitate, 4500 rev/mins of centrifugal 12min, collecting precipitation, 60Pa~100Pa vacuum freeze-drying 5h obtains the banana oligosaccharide product;
(2) residual pomace is used for enzymolysis and prepares the solvable and soluble dietary fiber of banana after the lixiviate banana liquid glucose:
Steps A: in pomace, add amylase, diastatic consumption is 4.0% of a pomace weight, 95 ℃ of operative temperatures, and it is 20mmol/L that adding calcium chloride makes calcium ion concentration, by adding hydrochloric acid and sodium hydroxide solution adjusting pH value is 4.5,100 ℃ of water-baths vibration enzymolysis 8h;
Step B: add protease in the steps A enzymolysis liquid, the consumption of protease is 0.25% of a pomace weight, and 55 ℃ of temperature are 7.5,50 ℃ of water-baths vibration enzymolysis 2.2h by adding hydrochloric acid and sodium hydroxide solution adjusting pH value;
At the centrifugal 14min of 3500 rev/mins rotating speed, freeze-drying 6h gets banana insolubility dietary fiber under the isolated 80Pa of being deposited in~150Pa vacuum behind the enzymolysis; The absolute ethyl alcohol that adds 4 times of supernatant volumes in the supernatant must precipitate, and is deposited in that freeze-drying 6h gets banana soluble dietary fiber finished product under 80Pa~150Pa vacuum;
(3) compound sugar product and solvable, insolubility dietary fiber finished product are to mix at 2: 2: 2 to obtain described composition 2 according to portfolio ratio.
Embodiment 3
(1) banana peeling; Pulp making beating 2min, flooding, the feed liquid w/v is 1g: 1ml, and temperature is 55 ℃, and extraction time is 55min, extracts 5 times; Merge No. 5 times extract, pomace is put in addition, and extract concentrates through suction filtration and vacuum, with the absolute ethyl alcohol of 4 times of extracting liquid volumes precipitate, 4000 rev/mins of centrifugal 13min, collecting precipitation, 100Pa~150Pa vacuum freeze-drying 4h obtains the banana oligosaccharide product;
(2) residual pomace is used for enzymolysis and prepares the solvable and soluble dietary fiber of banana after the lixiviate banana liquid glucose:
Steps A: in pomace, add amylase, diastatic consumption is 3.8% of a pomace weight, 98 ℃ of operative temperatures, and it is 18mmol/L that adding calcium chloride makes calcium ion concentration, by adding hydrochloric acid and sodium hydroxide solution adjusting pH value is 4.2,100 ℃ of water-baths vibration enzymolysis 9h;
Step B: add protease in the steps A enzymolysis liquid, the consumption of protease is 0.22%, and 53 ℃ of temperature are 7.2,55 ℃ of water-baths vibration enzymolysis 2h by adding hydrochloric acid and sodium hydroxide solution adjusting pH value;
At the centrifugal 12min of 4500 rev/mins rotating speed, freeze-drying 6h gets banana insolubility dietary fiber under the isolated 80Pa of being deposited in~150Pa vacuum behind the enzymolysis; The absolute ethyl alcohol that adds 4 times of supernatant volumes in the supernatant must precipitate, and is deposited in that freeze-drying 6.5h gets banana soluble dietary fiber finished product under 90Pa~120Pa vacuum;
(3) compound sugar product and solvable, insolubility dietary fiber finished product are 2: 2: 2 according to mass ratio, mix obtaining described composition 3.
Embodiment 4 zooperies
Experimental technique is with reference to " health food check and assessment technique enforcement of regulations handbook (publishing in 2003), " the bowel relaxing functions method of inspection ".Animal: SPF level Kunming mouse, male, purchase in Guangdong Medical Lab Animal Center animal credit number SCXK 2003-0002,2007A006.
Experimental result is shown in Table 1:
The influence of table 1 pair small intestine prepared Chinese ink propelling rate A)
Group Dosage g/ (kgBWd) Prepared Chinese ink propelling rate P (%)
The blank group ??0 ??76.6±5.8 *
Compound diphenoxylate constipation model control group ??0.005 ??44.7±5.4
Simple banana oligosaccharide product ??2.5 ??70.8±8.0 *
Simple banana soluble dietary fiber finished product ??1.0 ??50.9±12.0
Simple banana soluble dietary fiber finished product ??2.0 ??62.6±11.8 *
Simple banana insolubility dietary fiber finished product ??2.0 ??54.7±15.2
Composition 1 Compound sugar 2.5 solvable dietary fiber 1 insolubility dietary fibers 2 ??60.2±4.4 *
Group Dosage g/ (kgBWd) Prepared Chinese ink propelling rate P (%)
Composition 2 Compound sugar 2 ??59.2±7.3 *
Solvable dietary fiber 2 insolubility dietary fibers 2
A) compare with the constipation model control group *P<0.05.
Zoopery shows, under present composition effect, can make mouse small intestine prepared Chinese ink propelling rate reach 60.2%~66.5%, is significantly higher than the propelling rate 44.7% of compound diphenoxylate constipation model control group.

Claims (9)

1. banana oligosaccharide and diet fiber composition, it is characterized in that comprising banana oligosaccharide, soluble dietary fiber and insolubility dietary fiber, described banana oligosaccharide, soluble dietary fiber and insolubility dietary fiber quality ratio are 2.5: 1: 2 or 2: 2: 2.
2. described preparation of compositions method of claim 1 is characterized in that may further comprise the steps:
(1) banana pulp making beating, flooding, pomace is put in addition, precipitates extract with absolute ethyl alcohol, collecting precipitation, the vacuum freeze-drying precipitation prepares banana oligosaccharide;
(2) add enzyme in the pomace of putting in addition after step (1) lixiviate and carry out enzymolysis, centrifugation behind the enzymolysis, precipitation gets banana insolubility dietary fiber through vacuum freeze-drying; Supernatant precipitates through absolute ethyl alcohol, vacuum freeze-drying precipitate soluble dietary fiber;
(3) proportionally mix banana oligosaccharide, soluble dietary fiber and insolubility dietary fiber and get described composition.
3. preparation method according to claim 2 is characterized in that the described beating time of step (1) is 2~4min; The feed liquid w/v of described flooding is 1g: 1ml, and extraction temperature is 50~60 ℃, and extraction time is 50~60min; The consumption of absolute ethyl alcohol is 4 times of extracting liquid volumes; Described vacuum freeze-drying is freeze-drying 4~5h under 50Pa~150Pa.
4. preparation method according to claim 2 is characterized in that the described enzymolysis of step (2) comprises two steps:
Steps A: add amylase in pomace, diastatic consumption is 3.6~4.0% of a pomace weight, and 95~100 ℃ of operative temperatures are regulated calcium ion concentration 15~20mmol/L, and the pH value is 4~4.5, water-bath vibration enzymolysis 8~10h;
Step B: add protease in the mixture behind the steps A enzymolysis, the consumption of protease is 0.20~0.25% of a pomace weight, and 50~55 ℃ of operative temperatures, pH value are 7~7.5, water-bath vibration enzymolysis 2~2.5h.
5. preparation method according to claim 4 is characterized in that step (A) adds calcium chloride and regulates calcium ion concentration.
6. preparation method according to claim 2, the consumption that it is characterized in that the described absolute ethyl alcohol of step (2) is 4 times of supernatant volumes; Described vacuum freeze-drying is freeze-drying 6~7h under 80Pa~200Pa condition.
7. preparation method according to claim 2, it is characterized in that it being with the banana pulp 2.5min that pulls an oar, according to the feed liquid w/v is to add 60 ℃ of following lixiviate 50min of entry at 1: 1, extract 4 times, merge No. 4 times extract, concentrate the back through suction filtration and vacuum and precipitate with the absolute ethyl alcohol of 4 times of extracting liquid volumes, centrifugal collecting precipitation afterwards, 50Pa~80Pa vacuum freeze-drying 4h obtains the banana oligosaccharide product;
3.6% amylase, the calcium ion of 15mmol/L, adjust pH that pomace after the lixiviate adds pomace weight are 4,95 ℃ of water-baths vibration enzymolysis 10h, 0.2% protease, the adjust pH that add pomace weight then are 7,50 ℃ of water-bath vibration enzymolysis 2.5h, centrifugation behind the enzymolysis is deposited in 100Pa~200Pa vacuum freeze-drying 7h and gets banana insolubility dietary fiber; Supernatant adds 4 times of volume absolute ethyl alcohols and must precipitate, and is deposited in 100Pa~200Pa vacuum freeze-drying 7h and gets banana soluble dietary fiber finished product;
Is to mix at 2.5: 1: 2 to obtain described composition with compound sugar and solvable, insolubility dietary fiber according to portfolio ratio.
8. preparation method according to claim 2 is characterized in that it being with the banana pulp 3min that pulls an oar, and is to add entry 60 ℃ following lixiviate 50mins at 1: 1 according to the feed liquid w/v, extracts 4 times; Merge No. 4 times extract, concentrate the back through suction filtration and vacuum and precipitate with the absolute ethyl alcohol of 4 times of extracting liquid volumes, centrifugal collecting precipitation afterwards, 50Pa~80Pa vacuum freeze-drying 4h obtains the banana oligosaccharide product;
4.0% amylase, the calcium ion of 20mmol/L, adjust pH that pomace after the lixiviate adds pomace weight are 4.5,100 ℃ of water-baths vibration enzymolysis 8h, 0.25% protease, the adjust pH that add pomace weight then are 7.5,55 ℃ of water-bath vibration enzymolysis 2h, centrifugation behind the enzymolysis is deposited in 80Pa~150Pa vacuum freeze-drying 6h and gets banana insolubility dietary fiber; Supernatant adds 4 times of volume absolute ethyl alcohols and must precipitate, and is deposited in 1080Pa~150Pa vacuum freeze-drying 6h and gets banana soluble dietary fiber finished product;
Is to mix at 2: 2: 2 to obtain described composition with compound sugar and solvable, insolubility dietary fiber according to portfolio ratio.
9. the application of the described composition of claim 1 is characterized in that being applied to prepare bowel relaxing functions food, beverage or be used to prepare the medicine aspect for the treatment of constipation.
CN2009101943030A 2009-12-02 2009-12-02 Composition of banana oligosaccharide and dietary fibre, preparation method and application thereof Expired - Fee Related CN101715910B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2009101943030A CN101715910B (en) 2009-12-02 2009-12-02 Composition of banana oligosaccharide and dietary fibre, preparation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2009101943030A CN101715910B (en) 2009-12-02 2009-12-02 Composition of banana oligosaccharide and dietary fibre, preparation method and application thereof

Publications (2)

Publication Number Publication Date
CN101715910A true CN101715910A (en) 2010-06-02
CN101715910B CN101715910B (en) 2012-11-07

Family

ID=42430613

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2009101943030A Expired - Fee Related CN101715910B (en) 2009-12-02 2009-12-02 Composition of banana oligosaccharide and dietary fibre, preparation method and application thereof

Country Status (1)

Country Link
CN (1) CN101715910B (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102524701A (en) * 2012-02-17 2012-07-04 中国农业大学 Fruit residue dietary fiber and preparation method thereof
CN102630885A (en) * 2011-02-09 2012-08-15 张天平 Processing technology of fruit and vegetable dietary fiber
CN102640935A (en) * 2012-05-24 2012-08-22 中华全国供销合作总社济南果品研究院 Method for extracting dietary fiber in banana skin by adopting enzymic method
CN103355612A (en) * 2013-07-18 2013-10-23 华南理工大学 Making method of banana oligosaccharide-resistant starch combined food
CN105146271A (en) * 2015-09-22 2015-12-16 华南理工大学 Making method of banana resistant starch and dietary fiber fabricated food with weight-reducing function
CN108142825A (en) * 2017-12-22 2018-06-12 广西兴业百谷米业有限公司 A kind of dietary fiber rice of high-content and preparation method thereof
CN108514090A (en) * 2018-03-09 2018-09-11 华南理工大学 It is a kind of using banana as weight losing meal-replacing stick of primary raw material and preparation method thereof
CN115281341A (en) * 2022-08-12 2022-11-04 广西香蕉谷科技有限公司 Banana pill with intestine moistening function and preparation method thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1615745A (en) * 2003-11-14 2005-05-18 哈高科大豆食品有限责任公司 Recipe of soybean dietary fiber tablet
CN1864553A (en) * 2006-06-26 2006-11-22 杨公明 A method for preparing whole powder of banana
CN101456919A (en) * 2008-12-02 2009-06-17 王永昌 Method for preparing fast soluble brown alga dietary fiber oligosaccharide at high temperature

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102630885A (en) * 2011-02-09 2012-08-15 张天平 Processing technology of fruit and vegetable dietary fiber
CN102524701A (en) * 2012-02-17 2012-07-04 中国农业大学 Fruit residue dietary fiber and preparation method thereof
CN102524701B (en) * 2012-02-17 2013-09-18 中国农业大学 Fruit residue dietary fiber and preparation method thereof
CN102640935A (en) * 2012-05-24 2012-08-22 中华全国供销合作总社济南果品研究院 Method for extracting dietary fiber in banana skin by adopting enzymic method
CN103355612A (en) * 2013-07-18 2013-10-23 华南理工大学 Making method of banana oligosaccharide-resistant starch combined food
CN105146271A (en) * 2015-09-22 2015-12-16 华南理工大学 Making method of banana resistant starch and dietary fiber fabricated food with weight-reducing function
CN105146271B (en) * 2015-09-22 2018-10-16 天蕉健康食品(广东)有限公司 A kind of production method of banana resistant starch and dietary fiber composition food with weight losing function
CN108142825A (en) * 2017-12-22 2018-06-12 广西兴业百谷米业有限公司 A kind of dietary fiber rice of high-content and preparation method thereof
CN108514090A (en) * 2018-03-09 2018-09-11 华南理工大学 It is a kind of using banana as weight losing meal-replacing stick of primary raw material and preparation method thereof
CN115281341A (en) * 2022-08-12 2022-11-04 广西香蕉谷科技有限公司 Banana pill with intestine moistening function and preparation method thereof

Also Published As

Publication number Publication date
CN101715910B (en) 2012-11-07

Similar Documents

Publication Publication Date Title
CN101715910B (en) Composition of banana oligosaccharide and dietary fibre, preparation method and application thereof
CN101606704B (en) Dietary fiber food with various functional actions
KR101738912B1 (en) Composition For Anti-obesity And Diet
CN106605923A (en) Compound dietary fiber and preparation method thereof
CN111387381A (en) Lonicera edulis compound fruit juice beverage and preparation method thereof
CN105558737A (en) Sugar-free edible fungus solid drink and preparation method thereof
CN101238893B (en) High edible fibre chickpea nutritive powder and preparation and application thereof
CN107593925A (en) A kind of black soya bean black fungus soymilk and preparation method thereof
CN110192583A (en) With the composition and application for promoting intestinal health, control weight and raising to be immunized
CN109699745A (en) A kind of fucosylated oligosaccharide baby formula milk powder and preparation method thereof
CN104872775B (en) A kind of compound fat-reducing solid beverage of green tea tea polysaccharide and application
CN101709095B (en) Banana ligoglucoside and preparation method and application thereof
CN106805253A (en) A kind of banana extract for relief of constipation and preparation method thereof
CN108782770A (en) A kind of Siraitia grosvenorii almond milk and preparation method thereof
CN103504070A (en) Arctium lappa kidney nourishing tea and preparation method thereof
CN112120137A (en) Production method of novel dietary fiber solid beverage
CN101972019B (en) Facial beautification and health care function beverage made of radix polygonati officinalis and production method thereof
KR100994196B1 (en) Method of preparing fermented liquid of garlic and chonggugjang
CN102599512B (en) Sea buckthorn nutrition powder and preparation method thereof
CN104366356A (en) Method for preparing functional nourishment by using deep-processed sweet potatoes
CN101569645A (en) Smallanthus sonchifolius buccal tablet and preparation method thereof
CN108741046B (en) Tamarind crystal beer cake and preparation method thereof
JP2009034017A (en) Diet food
CN101797049A (en) Nutrient eight-treasure soup formula and preparation process thereof
KR20210041387A (en) A natural tea removing the effect of hangover contained the extract material in arrowroot blossoms and Plantago ovata

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20121107

Termination date: 20181202

CF01 Termination of patent right due to non-payment of annual fee