CN110192583A - With the composition and application for promoting intestinal health, control weight and raising to be immunized - Google Patents
With the composition and application for promoting intestinal health, control weight and raising to be immunized Download PDFInfo
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Abstract
The present invention provides a kind of with the composition for promoting intestinal health, control weight and raising to be immunized and application, and the main component of the composition is circle luxuriant Cillium, Euglena, gumbo powder, loquat flower, oligofructose, matcha powder.Its raw material is prepared with parts by weight, circle bud 20-35 parts of Cillium, 2-10 parts of Euglena, 1-10 parts of gumbo powder, 1-10 parts of loquat flower, 40-55 parts of oligofructose, 10-20 parts of matcha powder.The present invention largely studies the type and proportion of each raw material, and finally obtained composition is conducive to improve constipation maintenance intestinal health；Increase satiety and controls weight；And immune disease-resistance ability is improved, it solves the problems, such as that existing replenishers improve intestinal health, control weight and improve and is immunized and can not get both.In addition, there is composition of the invention faint scent Matcha widely to receive, and natural without side-effects, using safe and effective, market application prospect is wide.
Description
Technical field
The invention belongs to food or health product technology fields, and in particular to one kind, which has, promotes intestinal health, control weight
Immune composition and its application with raising.
Background technique
Current quick rhythm of life, so that it is exceptionally prevailing to take out fast food culture, however to take out that there is: heats excessively high,
The problems such as the few, unbalanced nutrition of dietary fiber.
Firstly, the excessive food total amount of heat of dietary intake is excessively high, the metabolic diseases such as obesity is caused to get worse.From Britain
The researchers of London Imperial College School of Public Health deliver on " lancet " one studies have shown that human society
The problem of obesity faced is increasingly serious, estimates that the fat number in the whole world rises to 6.41 hundred million in 2014 from 1.05 hundred million in 1975
People.Therefore, caloric intake is reduced, weight is controlled, it is extremely urgent to reduce metabolic disease risk.
It is well known that by increasing satiety, inhibiting fat absorption that can effectively control weight.Circle bud isapgol husk has
Very strong water absorbing capacity, into stomach after water swelling, increase satiety, reduce dinner food-intake.The gumbo that gumbo powder contains
Polysaccharide can inhibit the absorption of food-borne fat, to realize the effect of control weight.
Secondly, dietary fiber intake is very few, intestines peristalsis slows down, enteric flora disturbance, will lead to constipation, diarrhea, belch
Etc. a series of intestines problems.Display in " 2012 national intestinal health white paper ", there are bowel problems by nearly 95% compatriots, therefore again
Intake depending on dietary fiber in diet and prebiotics is extremely important.About dietary fiber recommended intake standard, Chinese nutrition
In the DRIs that can be released, fix tentatively Chinese residents intake dietary fiber suitable recommended amounts are as follows: it is high, medium and low be respectively 35,30,
25g/d。
Circle bud isapgol husk dietary fiber content is up to 80%, is capable of increasing the volume of excreta, enhances intestines peristalsis, change
Kind constipation problem.In addition, oligofructose can effectively facilitate intestinal beneficial bacterium proliferation, inhibit to have as generally acknowledged " prebiotics "
Evil bacterium, balanced intestinal flora improve intestinal health.
Finally, unbalanced nutrition, influences body's immunity, disease resistance dies down.The immunity of human body depends on mostly
Gene, but the influence of environment is also very big, such as diet, sleep, movement, pressure.Wherein diet has conclusive shadow
Power is rung, because the ingredient of some foods can assist in stimulation immune system, enhances immunocompetence.Prebiotics balance intestinal flora,
Be conducive to be promoted immune.In addition, polysaccharide, Flavonoid substances are also adjustable immune, disease resistance is promoted.
As stated above, control volume can be helped by reducing the nutritious food of intake heat, increase dietary fiber, supplement
Weight improves intestinal health and promotes immunity.However product on the market can not meet above many requirements simultaneously at present, therefore open
To send out satiety a kind of strong, promote intestinal health, improve immunity and product that mouthfeel is good is highly desirable.
Summary of the invention
The present invention a kind of promotion intestinal health being free from side effects, control weight are provided and improve immune function composition and
Using the composition contains the ingredients such as the luxuriant Cillium of circle, Euglena, gumbo powder, loquat flower, oligofructose, matcha powder.Invention
Purpose is to solve the problems, such as that existing replenishers improvement intestinal health, control weight and raising immunity are undesirable.
The present invention adopts the following technical scheme that achieve the goals above
One kind, which has, to be promoted intestinal health, control weight and improves immune function composition, and the composition includes following parts by weight
Raw material components: circle bud 20-35 parts of Cillium, 2-10 parts of Euglena, 1-10 parts of gumbo powder, 1-10 parts of loquat flower, oligomeric fruit
It is 40-55 parts sugared, 10-20 parts of matcha powder.
Further, the composition also contains additive, the additive selection sweetener, anticaking agent one kind or
It is a variety of.
Further, the sweetener is in steviol glycoside, Aspartame, Sucralose, acesulfame potassium, Fructus Momordicae fructoside
One or more；Selected anticaking agent is selected from one of tricalcium phosphate, silica, microcrystalline cellulose, potassium ferrocyanide
Or it is a variety of.
Further, the composition are as follows: circle bud 20-35 parts of Cillium, 2-10 parts of Euglena, 1-10 parts of gumbo powder,
1-10 parts of loquat flower, 40-55 parts of oligofructose, 10-20 parts of matcha powder, 0.1-0.5 parts of acesulfame potassium, 0.1-0.5 parts of Sucralose,
- 5 parts of silica 1.
Further, the composition are as follows: circle bud 25-30 parts of Cillium, 3-8 parts of Euglena, 1-5 parts of gumbo powder, Pi
Rake spends 1-5 parts, 45-55 parts of oligofructose, 10-17 parts of matcha powder, 0.1-0.3 parts of acesulfame potassium, 0.1-0.3 parts of Sucralose, two
1-3 parts of silica.
The composition is to improve constipation to safeguard intestinal health；Increase satiety and controls weight；And improve immune disease-resistance ability
Preparation.
Beneficial effects of the present invention
In the present invention, circle bud Cillium, oligofructose improve intestinal health；Gumbo powder inhibits absorption of dietary fat；Euglena,
Loquat flower promotes immune function.Each component mutually cooperates with, unbalanced so as to improve fast food race diet nutritional, resistance decline to ask
Topic.Matcha powder neutralizes Euglena fishy smell well, receives mouthfeel widely.
The effect of each raw material in the present invention is described below:
Gumbo powder: extracting from okra, belongs to Malvaceae Abelmoschus annual herb plant, rich in viscous in tender okra pod
Liquid substance, mainly by the mixture of araban, galactan, Pectic polysaccharides and a small amount of glycoprotein.This is also the yellow autumn
The more prominent feature of certain herbaceous plants with big flowers, mucus can help digest, and prevent constipation, protect stomach, and can enhance physical endurance.Pectin class is more
Sugar is Soluble Fiber, and water swelling increases satiety, reduces intake heat.In addition to this, gumbo polysaccharide can effectively inhibit
The absorption of food fat, cardinal principle be polysaccharide retention stomach in dietary fat or in another manner with the dietary fat knot in stomach
It closes, forms indigestible polysaccharide-fat compound, be discharged as waste from body, to reach control weight, toxin expelling is supported
The effect of face.
Loquat flower: loquat flower is rosaceae Eriobotrya loquat Eriobotrya japonica (Thunb) Lindl.
Dry flower.Loquat is one of China specialty fruit tree, is recorded in Western Han Dynastry's Si Maxiangru works " upper woods is assigned " earliest, has 2000
The cultivation history in year.Loquat medicinal part has loquat flower, loquat, loquat seed, loquat phloem, loguat leaf, loquat root.Pi
Rake flower effective component is mainly triterpene and flavone compound, has very high medicinal, edible value, can use drug, food, guarantor
The exploitation in strong, daily-use chemical industry field.The triterpenes main component ursolic acid and oleanolic acid that loquat flower contains have significant
Cough and asthma relieving efficacy.In addition to this, the Flavonoid substances that loquat flower contains have good immunological regulation and anti-oxidant anti-ageing
Effect.Loquat flower (defends the new Shen word (2017) the 0004th of food) in publicity at present, without edible taboo.
Circle bud Cillium: being the luxuriant Chinese herbaceous peony (Classification system: Plantago of Plantaginaceae Plantago circle of artificial growth
Ovata) the shell of seed, China defend planning commission's bulletin (2014 No. 10) and ratify it as new resource food raw material.Circle bud Chinese herbaceous peony
It is rich in dietary fiber in sub- shell, up to 80% or more, and wherein the ratio of soluble dietary fiber and insoluble diedairy fiber is high
Up to 7:3.High-content dietary fiber can increase satiety and reduce food intake, and help controls weight.In addition, circle bud plantasan
Water swelling can increase faecal volume, promote defecation.FDA is mentioned in allowing to state: (the circle bud Chinese herbaceous peony of daily 7 g or more
Sub- shell source) soluble dietary fiber, cholesterol can be reduced, risk of cardiovascular diseases is reduced.
Euglena: also known as green worm algae.China defends planning commission's bulletin (2013 No. 10) and ratifies it as new resource food raw material.It is green
Worm algae was just lived on earth before 500,000,000 years, and body is about the fine algae of 0.05mm.Because being in the bottom of food chain, make
It is obtained to accumulate entirely without toxin.Green worm algae possesses up to 59 kinds of nutritional ingredients, and digestibility is up to 93.1%, amino acid score (AAS)
100) up to 83(full marks are that exclusive ingredient Paramylon (Euglena polysaccharide), which has, to be adjusted immunity, adjusts blood glucose, conditioning
Stomach improves constipation, inhibits hepar damnification, alleviates the effects of gout.
Oligofructose (FOS): also referred to as fructo-oligosaccharide is to be present in water fruits and vegetables, the natural work in honey and other substances
Sexual element.The oligofructose made of modern biotechnology transfructosylase converts and refines, is concentrated, it is excellent with its
Physiological function more becomes international food pandemic functional food ingredient in the market nearly ten years.As generally acknowledged " benefit
Raw member ", oligofructose can effectively play the physiological activity of probiotics, selectively increase Microflora, be the growth of probiotics
Promotive factor.In addition, oligofructose can promote intestinal flora balance as a kind of dietary fiber, improves gastrointestinal function, have
Relax bowel and defecation, adjusts the different physiological roles such as blood glucose at reducing blood lipid.
Matcha powder: matcha powder has long edible history in China, is to be milled into micro mist shape, covering with natural stone mill
, steam green green tea.General green tea tea tree contacts illumination in growth period whole process, and the characteristic for grinding tea is before picking at least
Steam after 20 days 90% shade cultures and picking removes chlorophyll.Treated in this way, and tealeaves color is green, and bitter taste is light, also slightly green
Careless gas, wherein protein, amino acid and leaf green content are very high.Smearing tea not only has good mouthfeel, is chiefly used in sweets tea ceremony,
In addition to this, some researches show that harmful free radicals excessive in the tea polyphenols energy removing machine body in matcha powder, to protect and repair
Multiple antioxidant system has remarkable result to enhancing immunity of organism, anti-resisting cancer and senility.
The embodiment of the present invention has the advantages that
1. the luxuriant Cillium of circle disclosed by the invention has very strong water imbibition, enable excrement soft or hard suitable by the effect of osmotic pressure
Degree, is smoothly discharged, and improves constipation.Grain of the oligofructose as intestine beneficial bacteria colony, balance intestinal flora.The two synergistic effect
Adjust intestinal health significant effect.
2. composition disclosed by the invention has stronger satiety, and gumbo powder inhibits fat absorption, helps control volume
Weight, reduces the generation of metabolic disease.
3. Euglena polysaccharide and loquat flower Flavonoid substances adjust immune, enhancing disease resistance.
4. composition disclosed by the invention is the Matcha of faint scent, widely big well-established.
Specific embodiment
In order to better illustrate the present invention, technical solution in the embodiment of the present invention is clearly and completely retouched below
It states, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the present invention
In embodiment, every other implementation obtained by those of ordinary skill in the art without making creative efforts
Example, shall fall within the protection scope of the present invention.
Embodiment 1
A kind of composition being immunized with promotion intestinal health, control weight and raising, the composition is by following components by weight percent group
At: circle bud 35 parts of Cillium, 10 parts of Euglena, 10 parts of gumbo powder, 10 parts of loquat flower, 55 parts of oligofructose, 20 parts of matcha powder.
In the present embodiment, circle bud Cillium (Ministry of Public Health 2014 No. 10) dietary fiber content is up to 80% or more,
Satiety and promotion intestines peristalsis effect are obvious.
In the present embodiment, the beta glucan content that Euglena (Ministry of Public Health 2013 No. 10) contains is up to 58%, and on the market
Most of beta-dextran content can only achieve 25%, therefore it is significant to promote immune function.
Embodiment 2
A kind of composition being immunized with promotion intestinal health, control weight and raising, the composition is by following components by weight percent group
At: circle bud 30 parts of Cillium, 5 parts of Euglena, 2 parts of gumbo powder, 2 parts of loquat flower, 50 parts of oligofructose, 15 parts of matcha powder.Its
Remaining same embodiment 1.
Embodiment 3
A kind of composition being immunized with promotion intestinal health, control weight and raising, the composition is by following components by weight percent group
At: circle bud 25 parts of Cillium, 4 parts of Euglena, 1 part of gumbo powder, 1 part of loquat flower, 45 parts of oligofructose, 10 parts of matcha powder.Its
Remaining same embodiment 1.
Embodiment 4
A kind of composition being immunized with promotion intestinal health, control weight and raising, the composition is by following components by weight percent group
At: circle bud 20 parts of Cillium, 2 parts of Euglena, 1 part of gumbo powder, 1 part of loquat flower, 40 parts of oligofructose, 10 parts of matcha powder.Its
Remaining same embodiment 1.
Embodiment 5
A kind of composition being immunized with promotion intestinal health, control weight and raising, the composition is by following components by weight percent group
At: circle bud 35 parts of Cillium, 10 parts of Euglena, 10 parts of gumbo powder, 10 parts of loquat flower, 55 parts of oligofructose, 20 parts of matcha powder,
0.5 part of acesulfame potassium, 0.5 part of Sucralose, 5 parts of silica.Remaining is the same as embodiment 1.
Embodiment 6
A kind of composition being immunized with promotion intestinal health, control weight and raising, the composition is by following components by weight percent group
At: 30 parts of Cillium of circle bud, 5 parts of Euglena, 2 parts of gumbo powder, 2 parts of loquat flower, 50 parts of oligofructose, 15 parts of matcha powder, peace
Match sweet 0.1 part, 0.1 part of Sucralose, silica 1 part.Remaining is the same as embodiment 1.
Embodiment 7
A kind of composition being immunized with promotion intestinal health, control weight and raising, the composition is by following components by weight percent group
At: 20 parts of Cillium of circle bud, 2 parts of Euglena, 1 part of gumbo powder, 1 part of loquat flower, 40 parts of oligofructose, 10 parts of matcha powder, peace
Match sweet 0.1 part, 0.1 part of Sucralose, silica 1 part.Remaining is the same as embodiment 1.
Embodiment 8
A kind of composition being immunized with promotion intestinal health, control weight and raising, the composition is by following components by weight percent group
At: 30 parts of Cillium of circle bud, 8 parts of Euglena, 5 parts of gumbo powder, 5 parts of loquat flower, 55 parts of oligofructose, 17 parts of matcha powder, peace
Match sweet 0.3 part, 0.3 part of Sucralose, 3 parts of silica.Remaining is the same as embodiment 1.
Embodiment 9
A kind of composition being immunized with promotion intestinal health, control weight and raising, the composition is by following components by weight percent group
At: 30 parts of Cillium of circle bud, 5 parts of Euglena, 2 parts of gumbo powder, 2 parts of loquat flower, 50 parts of oligofructose, 15 parts of matcha powder, peace
Match sweet 0.1 part, 0.1 part of Sucralose, silica 1 part.Remaining is the same as embodiment 1.
Embodiment 10
A kind of composition being immunized with promotion intestinal health, control weight and raising, the composition is by following components by weight percent group
At: 25 parts of Cillium of circle bud, 3 parts of Euglena, 1 part of gumbo powder, 1 part of loquat flower, 45 parts of oligofructose, 10 parts of matcha powder, peace
Match sweet 0.1 part, 0.1 part of Sucralose, silica 1 part.Remaining is the same as embodiment 1.
Embodiment 11
A kind of application with the composition for promoting intestinal health, control weight and raising to be immunized, the composition are to improve constipation
Safeguard intestinal health；Increase satiety and controls weight；And improve the preparation of immune disease-resistance ability.
Below by pharmacodynamic study result, the invention will be further described.
First part's intestinal health experimental study
Experiment one: Small intestine advance activity experiment
1. experimental principle
The function of mouse small intestine is detected, the method generallyd use is the ink progradation for measuring regular hour small intestine.This experiment
The method of use is first to be established mouse wriggling with compound diphenoxylate modeling and inhibit model, then surveyed mouse small intestine after intervening
Ink progradation.This index can measure the case where mouse intestinal function.
2. experimental animal
SPF grades of Kunming kind healthy male mices 60 are selected, animal is randomly divided into 6 groups by weight, every group 10, divides
Harmonious inspection carried out to weight when group, each group is respectively model control group, blank control group, solid made from embodiment 1-4
Body beverage intervention group.It weighs and records after grouping.
3. laboratory sample
The composition matched according to embodiment, compound diphenoxylate suspension, prepared Chinese ink etc..
4. experimental method
Each group gives basal feed.Model control group, blank control group give physiological saline stomach-filling.Intervention group is given accordingly respectively
Solid beverage by test product stomach-filling, dosage is 15 times of the human body amount of being recommended to use, i.e. 2 .5g/kgBW, 60Kg adult push away daily
Recommending usage amount is 10g, is configured to aqueous solution by test product before stomach-filling.Ad lib during test, record each group mouse weight become
Change.
After continuous feeding 15d, 16h fasting (period free water) before testing.Model control group and 4 intervention groups are small
Mouse is given 5 mg/kg BW compound diphenoxylates in a manner of stomach-filling and makes enterocinesia inhibition model, and blank control group is given
Water replaces.
After 30 min of modeling, 4 intervention group stomach-fillings give containing tested material prepared Chinese ink suspension (containing 5% active carbon, 10%
Arabia gum), blank control group and model control group fill give blank prepared Chinese ink respectively, 20 ml/kgBW of stomach-filling amount.25
Cervical dislocation puts to death animal after min, opens abdominal cavity and separates mesenterium, clip upper end from the intestinal tube of pylorus, lower end to ileocecus,
On tray, small intestine is gently pulled into line measurement.Length of intestine is " total small intestinal length ", and pylorus to prepared Chinese ink forward position is " ink
Juice promotes length ", ink progradation calculation method: ink progradation=prepared Chinese ink promotes length/total small intestinal length × 100%.
5. experimental result
Experimental data is indicated with mean ± standard deviation, and with SPSS17 .0 software statistics, comparison in difference is examined using t between each group
Method, 0 .05 of P < are that difference has statistical significance.
The experiment of 5.1 Small intestine advance activities
Table 1
Note: compared with blank control group, △ △ P < 0.01;Compared with model control group, * * P < 0.01.
As shown in Table 1, model group ink progradation is significantly lower than blank group (P < 0.01), illustrates that prepared Chinese ink Promoting Experiment is small
Mouse Constipation Model modeling success.After stomach-filling 15d, compared with model group, the Intestinal propulsive rate of embodiment intervention group dramatically increases (P
< 0.01), illustrate that intervention group has and promote intestines peristalsis, relief of constipation improves the effect of intestinal health.
Experiment two: the present composition promotes immune function experiment
1. experimental principle
Mouse immune ability is detected, from cellular immune function, humoral immune function, monocytes/macrophages function, NK cell activity
Four aspects are verified respectively.
2. experimental animal
SPF grades of Kunming kind healthy male mices 150 are selected, animal is randomly divided into 3 big groups by weight, and every group 50,
3 big groups are respectively to be immunized 1 group, are immunized 2 groups, are immunized 3 groups.
3. laboratory sample
Sheep red blood cell (SRBC) (SRBC), Hank ' s liquid (pH value 7.2~7.4), RPMI1640 culture solution, calf serum are green
Streptomysin, concanavalin A (ConA), MTT, complement (guinea pig serum), SA buffer, india ink, Dou Shi reagent, YAC-1
Cell etc..
4. experimental method
Mouse is divided into 3 big groups, and 1 group of measurement delayed allergy (DTH) and antibody-producting cell number is immunized；It is 2 groups immune
Carry out mouse lymphocyte transformation experiment, the NK cytoactive detection of ConA induction；Immune 3 groups of carry out carbonic clearances are real
It tests.Adaptable fed 3 days.
5 groups are randomly divided into per 50 mouse of group greatly, is blank control group, model control group and the embodiment of the present invention respectively
5-7 intervention group.Blank control group, model control group give 1mL/10g distilled water；Intervention group gives same volume embodiment 5-7 group
Close object.Continuous gavage 15d.Experiment terminates preceding 10 d, and intervention group, model group mouse peritoneal inject 80 mg of cyclophosphamide Injection
/ kg, continuous 3 d of sensitization, causes immunologic hypofunction mouse.Isometric physiological saline (0.1 mL/ is injected intraperitoneally in blank group
10g)。
4.1 cellular immune function
4.1.1 delayed allergy (DTH) (vola pedis thickens method): mouse peritoneal is given to inject 2% hematocrit SRBC (0.2ml/
Every mouse) after sensitization 4 days, left back vola pedis thickness is measured, 20% (v/v) SRBC (20 μ then are subcutaneously injected in measuring point
The every mouse of l/), measure left back vola pedis thickness for 24 hours after injection, same position measurement three times, is averaged, to attack front and back
Vola pedis thickness difference (swelling degree of the paw) indicates the degree of DTH.
4.1.2 the mouse lymphocyte conversion test (MTT method) of ConA induction: it is sterile to take spleen, equipped with sterile
In Hank ' s liquid plate, spleen is ground with tweezers, cell suspension is made, 200 mesh screens are washed 2 times with Hank ' s liquid,
Centrifugation 10min (1000r/min) every time, then takes precipitating to be suspended in the complete culture solution of l mL, platform phenol orchid dyeing counting
(should be 95% or more).Cell concentration is adjusted to 3 × 10^6/ml, is then divided to two holes to be added 24 cell suspension
In well culture plate, every hole 1m1 a, hole adds 75 μ l ConA liquid, and another hole compares, and sets 5% CO2 37 DEG C of incubator cultures
72h.Culture terminates preceding 4h, and every hole gently sucks supernatant 0.7ml, and the RPMI1640 that 0.7ml is free of calf serum is added
Culture solution, while 50 hole μ l/ MTT (5mg/ml) is added and continues to cultivate 4h, after culture, it is different that 1ml acidity is added in every hole
Propyl alcohol is completely dissolved purple crystal, is then dispensed into 96 well culture plates, and 3 parallel holes are done in every hole, with 2010 type microplate reader,
OD value is measured with 570nm wavelength.The proliferative capacity of lymphocyte with plus the A value in the hole ConA subtract ConA be not added
The A value in hole indicates.
4.2 humoral immune function
4.2.1 antibody-producting cell detection (Jerne improves slide method): every mouse peritoneal injects 2% hematocrit SRBC
0.2ml, the mouse cervical dislocation after being immunized 5 days are put to death, and are taken out spleen and are placed in the plate for filling Hank ' s liquid, grind
Cell suspension is made in spleen, through 200 mesh net filtrations, is centrifuged (1000r/min) 10min, washes 2 with Hank ' s liquid
Time, finally cell is suspended in 5ml RPMI1640 culture solution, platform phenol orchid dyeing counting (should be 95% or more), and
Cell concentration is adjusted to 5 × 106/ml, splenocyte suspension is made.Agarose is configured to 1% aqueous solution, water-bath is boiled
30min is mixed with double concentration Hank ' the s liquid of equivalent, dispenses small test tube, every pipe 0.5ml, then add 10% (to use into pipe
SA buffer) hematocrit SRBC 50 μ l, each 20 μ l of splenocyte suspension do two Duplicate Samples, after mixing rapidly, topple over
In on agarose thin layer slide, after agar solidification, slide level is buckled and is placed on horse, is put into carbon dioxide incubator and incubates
1.5h is educated, then complement is added in glass frame groove, continues to incubate 1.5h, counts hemolysis plaque number.It is prepared by complement
Guinea pig blood is acquired, serum (pooled serum of at least 5 cavys) is isolated, 1ml hematocrit SRBC is added to 5ml
In guinea pig serum, 4 DEG C of refrigerators place 30min, often vibrate, centrifuging and taking supernatant, packing, -70 DEG C of preservations.Used time is buffered with SA
Liquid is by 1: 15 dilution.
4.3 monokaryons-macrophage phagocytic function
Detection mouse monokaryon-macrophage phagocytic function is tested using mouse carbonic clearance.With the g dosage of 0.1 mL/10 warp
25% india ink of mouse tail vein injection takes blood from mouse orbit veniplex respectively in the 5th min, 20 min after injection
25 μ l are dissolved in the Na of 2 mL 0. 1%2CO3It is shaken up in solution, with 0.1% Na2CO3As blank control, spectrophotometric is used
Count 600 nm measurement absorbance.Dissection mouse weighs liver, spleen weight amount, and index K is cleaned up in calculating and index α is cleaned up in correction.In formula
K = ( lgA1- lgA2) /( t2- t1) ;The weight of α=K1/3/(liver weight+spleen weight).Wherein A1, A2
Represent the absorbance successively surveyed twice, t2- t1Represent the time interval taken a blood sample twice.
4.4 NK cytoactive detections
NK cell activity is detected using lactic dehydrogenase (LDH) measuring method.YAC-1 cell is passed for 24 hours before experiment
It is commissioned to train feeding, is 4 × 10^5 mL -1 with RPMI1640 complete culture solution adjustment cell concentration.Animal gives nothing after medicine
Bacterium takes spleen, is placed in the small plate for filling appropriate sterile Hank ' s liquid, is gently ground spleen with tweezers, is made unicellular outstanding
Liquid.Through sieve pore internal diameter (75.0 ± 4.1) μm (200 mesh) the screen to filtrate, 0.5mL aqua sterilisa 20s splitting erythrocyte is added,
It is 2 × 107 mL -1 with RPMI1640 complete culture solution adjustment cell concentration.Take YAC-1 cell and splenocyte each
100 μ L are added in 96 well culture plates；Target cell Spontaneous release hole adds target cell and each 100 μ L of culture solution, and target cell is most
Big relief hole adds target cell and each 100 μ L of 2.5%Triton；Above-mentioned items are all provided with 3 multiple holes, in 37 DEG C, 5%CO2Training
It supports in case and cultivates 4h, 96 well culture plates are then centrifuged 5min, every 100 μ L of hole Aspirate supernatant with 1500rmin-1
In 96 well culture plate of horizontalization bottom, while 100 μ L of LDH matrix liquid is added, reacts 3min, 1molL-is added in every hole
1 hydrochloric acid solution, 30 μ L measures A value at microplate reader 490nm.
NK cell activity (%)=(reaction A value-Spontaneous release hole A value)/(maximum relief hole A value-Spontaneous release hole A value) ×
100%
5. experimental result
Data conversion and statistical analysis are carried out with Excel, SPSS10.0 software.After homogeneity test of variance is qualified, use
Dunnet single factor test Multiple range test statistical analysis.
5.1 cellular immune function
It in mouse delayed hair allergy experiment, is compared with blank control group, model group delayed allergy is significant
It reduces (P < 0.01).It compares and finds with model group, embodiment intervention group significantly improves mouse delayed allergy intensity
(P < 0. 01) illustrates that the present composition can increase mouse delayed allergy.It the results are shown in Table 2.
To the mouse spleen lymphocyte transformation experiment of ConA induction, intervention group A value difference value is higher than model control group, poor
It is different to have conspicuousness, show the ability that the present composition can improve mouse spleen lymphocyte conversion.It the results are shown in Table 2.
Table 2
Note: compared with blank control group, △ △ P < 0.01;Compared with model control group, * * P < 0.01；* P <
0.05。
5.2 humoral immune function
In the experiment of mouse antibodies cellulation, intervention group and model control group hemolysis plaque number difference have conspicuousness (P <
0.05), show that the present composition can be improved the antibody-producting cell number of mouse, the present composition is prompted to have enhancing small
The function of mouse humoral immunity.It the results are shown in Table 3.
Table 3
Note: compared with blank control group, △ △ P < 0.01;Compared with model control group, * * P < 0.01；* P <
0.05。
5.3 monokaryons-macrophage phagocytic function
In the experiment of mouse carbonic clearance, compared with blank control group, model control group immunologic hypofunction mouse carbon particle clearance function
It can significantly reduce (P < 0.01).Compared with model control group, intervention group mouse carbon particle clearance function significantly improves (P <
0.05) .Show that the present composition has the function of enhancing mouse monokaryon-macrophage phagocytic function, the results are shown in Table 4.
Table 4
Note: compared with blank control group, △ △ P < 0.01;Compared with model control group, * P < 0.05.
5.4 NK cytoactive detections
In the detection NK cell activity experiment of lactic dehydrogenase (LDH) measuring method, compared with blank control group, model control group
NK cell activity significantly reduces (P < 0.01).Compared with model control group, intervention group NK cells in mice activity is significantly improved
(P < 0.05).Show that the present composition has the function of enhancing mouse NK cell activity, the results are shown in Table 5.
Table 5
Note: compared with blank control group, △ △ P < 0.01;Compared with model control group, * * P < 0.01；* P <
0.05。
The present composition can significantly improve mouse delayed allergy intensity；The mice spleen of ConA induction can be enhanced
Lymphocyte proliferation ability has enhancing cellular immune function effect；The antibody-producting cell number for improving mouse, there is reinforcement
Liquid immunity function；There are enhancing mouse monokaryon-macrophage phagocytic function and enhancing mouse NK cell activity.
Show that the present composition has the function of certain strengthen immunity function.
Test the control weight experiment of three present compositions
1. experimental principle
The composition control weight and fat reducing effect are measured using prevention obese model method test mouse weight, body fat rate.
2. experimental animal
Select SPF grades of Kunming kind healthy male mices several (being greater than 50) only, rat feeding maintains feed under barrier system
Observation 7 days, is randomly divided into 2 groups by weight after the laundering period, maintains feed as blank control group wherein 10 rats are given,
Remaining mouse gives high-calorie feed as high lipid food group.It records weekly to appetite, berley amount, surplus appetite, weighs in 1
It is secondary.After feeding 2 weeks, high lipid food group rat is sorted by weight gain, eliminates the lower Diet resistant rat of weight gain.It will screening
The fat Sensitive Rats of 40 out are randomly divided into 4 groups by weight, every group each 10, respectively model control group and three embodiments
Intervention group.
3. laboratory sample
Maintain feed, high lipid food, suspension, the yellow Jackets of embodiment 8-10 preparation etc..
4. experimental method
After grouping, blank control group continues to give maintenance feed, and model control group and three intervention groups give the feeding of high heat model
Material, each intervention group gives the embodiment 8-10 composition suspension of preparation respectively, according to the volume stomach-filling of 1 .0mL/100g BW,
It feeds 6 weeks.Record is weighed in 1 time weekly.After the test, it weighs, 1% yellow Jackets (0 .5mL/100g BW) fiber crops
Liquor-saturated, solution takes perinephric fat, testis peripheral adipose, and weighs, and calculates rouge/body ratio.
5. experimental result
Experimental data is indicated with mean ± standard deviation, and with SPSS17 .0 software statistics, comparison in difference is examined using t between each group
Method, 0 .05 of P < are that difference has statistical significance.
Influence of 5.1 present compositions to mouse weight
By experimental data as can be seen that compared to the blank group, model group body weight obviously increases, there is significant difference (P <
0.01).Compared with model group, mouse weight starts the decline for statistical significance occur after present composition intervention 2 weeks, and 6 weeks
Intervention group weight is remarkably decreased afterwards, illustrates that the composition has the function of controlling weight.It the results are shown in Table 6.
Table 6
Note: compared with blank control group, △ △ P < 0.01;Compared with model control group, * * P < 0.01；* P <
0.05。
Influence of 5.2 present compositions to mouse body fat weight
By experimental data as can be seen that compared to the blank group, model group fat ratio obviously increases, there is significant difference (P
< 0.01).Compared with model group, mouse adipose is said than there is the reduction of statistical significance after present composition intervention 6 weeks
Bright the composition has the function of reducing fat.It the results are shown in Table 7.
Table 7
Note: compared with blank control group, △ △ P < 0.01;Compared with model control group, * P < 0.05.
Conclusion:
The embodiment of the present invention largely studies the type and proportion of each raw material of composition, finally obtained composition pair
In improving, bowel problems, control weight and raising immune effect are significant.Composition of the invention is naturally without side-effects, and use is safe
Effectively, market application prospect is wide.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention are fallen within the scope of the claimed invention.
Claims (6)
1. a kind of with promoting intestinal health, control weight and improve immune composition, which is characterized in that the composition includes
The raw material components of following parts by weight:
Circle bud 20-35 parts of Cillium, 2-10 parts of Euglena, 1-10 parts of gumbo powder, 1-10 parts of loquat flower, oligofructose 40-55
Part, 10-20 parts of matcha powder.
2. according to claim 1 have the composition for promoting intestinal health, control weight and raising to be immunized, feature exists
In the composition also contains additive, the one or more of the additive selection sweetener, anticaking agent.
3. according to claim 2 have the composition for promoting intestinal health, control weight and raising immune function, spy
Sign is, the sweetener be selected from one of steviol glycoside, Aspartame, Sucralose, acesulfame potassium, Fructus Momordicae fructoside or
It is a variety of；
Selected anticaking agent is selected from one or more of tricalcium phosphate, silica, microcrystalline cellulose, potassium ferrocyanide.
4. according to claim 1 have the composition for promoting intestinal health, control weight and raising to be immunized, feature exists
In the composition are as follows:
Circle bud 20-35 parts of Cillium, 2-10 parts of Euglena, 1-10 parts of gumbo powder, 1-10 parts of loquat flower, oligofructose 40-55
Part, 10-20 parts of matcha powder, 0.1-0.5 parts of acesulfame potassium, 0.1-0.5 parts, -5 parts of silica 1 of Sucralose.
5. according to claim 4 have the composition for promoting intestinal health, control weight and raising to be immunized, feature exists
In the composition are as follows:
Circle bud 25-30 parts of Cillium, 3-8 parts of Euglena, 1-5 parts of gumbo powder, 1-5 parts of loquat flower, 45-55 parts of oligofructose,
10-17 parts of matcha powder, 0.1-0.3 parts of acesulfame potassium, 0.1-0.3 parts, -3 parts of silica 1 of Sucralose.
6. according to claim 1 in 5 it is any it is described have promote intestinal health, control weight and improve immune composition
Using, it is characterised in that: the composition is to improve constipation to safeguard intestinal health；Increase satiety and controls weight；And it improves immune
The preparation of disease resistance.
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| CN110710660A (en) * | 2019-09-16 | 2020-01-21 | 湖南科尔生物技术有限公司 | Blood sugar lowering composition and dietary supplement containing bitter gourd powder |
| CN114451560A (en) * | 2022-02-21 | 2022-05-10 | 江中药业股份有限公司 | Dietary composition for diabetic patients and application thereof |
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Application publication date: 20190903 |