CN101715910B - Composition of banana oligosaccharide and dietary fibre, preparation method and application thereof - Google Patents
Composition of banana oligosaccharide and dietary fibre, preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a composition of banana oligosaccharide and dietary fibre, a preparation method and an application thereof. The composition comprises banana oligosaccharide, soluble dietary fibre and insoluble dietary fibre, wherein the proportion of the banana oligosaccharide to the soluble dietary fibre to the insoluble dietary fibre is 2.5:1:2 or 2:2:2.In the invention, the banana oligosaccharide is obtained by pulping the fruit flesh of bananas, leaching by water, precipitating by ethanol and freeze-drying in vacuum; the soluble and insoluble dietary fibre of the bananas is obtained by carrying out zymohydrolysis on the fruit residues of the bananas. The composition has the effect of relaxing bowels of a common purge, is mild, does not have stimulation property, overcomes side effects caused by common rhubarb and anthraquinone class purges, can exert the physiological function of preventing colonic and rectal cancers, cardiovascular diseases and the like of the dietary fibre and achieves the aims of retaining water, lubricating and keeping high penetrability; because the composition is natural, safe and effective, the composition is especially suitable for relieving the constipation symptoms of dry, dense and clotty stool and difficult defecation of infants because of eating powdered milk.
Description
Technical field
The invention belongs to food processing technology field, relate to a kind of functional food and preparation method thereof, be specifically related to a kind of banana oligosaccharide and diet fiber composition, its preparation method and application.
Background technology
Constipation is meant the ill state of defecation, comprises that the ight soil drying is discharged smooth and ight soil is not done two kinds of situation of also difficult discharge, refers generally to weekly defecation and is less than 2 ~ 3 times.Can be divided into organic constipation and functional consitipation according to the cause of disease, the former is caused by enteron aisle and systemic disease; The latter refers to that said clinically constipation mainly refers to functional consitipation because defecation reflex is not normal or block the colonic constipation that the proctogenous constipation cause and colonic peristalsis slowly cause.Rheum officinale, anthraquinone class cathartic are usually used in the treatment constipation; But this type of cathartic has side effect; Can make the patient that cathartic is produced dependence, the long-term use diminishes colon transfer function and enteron aisle nerve, can cause more serious functional, intractable constipation on the contrary; Produce the black change of colon, cause purgatives property colon.
Domestic the treatment constipation is mainly concentrated on zoopery and the clinical effectiveness and the mechanism research aspect of Chinese medicine compound prescription, the research of defaecation food rarely has report.
According to biochemistry noun validation board nomenclature, oligosaccharides is defined as the polymer that comprises 3～10 monose.To be monose couple together the low polymerization sugar with straight chain or branched chain that forms through glycosidic bond to oligosaccharides, therefore claims again " compound sugar ".Some can not be had following physiological function by the oligosaccharides that human consumption absorbs: the propagation of beneficial bacteria such as Bifidobacterium, Bacillus acidi lactici in (1) promotion human body, thus the growth of spoilage organisms in the inhibition enteron aisle improves the intestinal microecology environment; (2) in colon, produced SCFA,, reduce the pH value of enteron aisle like acetate, propionic acid, butyric acid etc. by fermentation; (3) improve immunity of organisms, reduce the concentration of poisonous tunning such as amine, phenol, the prevention colorectal cancer; (4) anti-treating constipation removed toxin; (5) increase the absorption that some must mineral element.
Dietary fiber mainly comprises cellulose, hemicellulose, pectin and hydrophilic colloid material, lignin and the material that is not decomposed by the human consumption enzyme.Different dietary fibers can be divided into soluble dietary fiber and insoluble diedairy fiber two big classes by dissolution characteristics, and the two merging is called total dietary fiber.Soluble dietary fiber is meant not by the human body alimentary canal enzymic digestion; But dissolving in warm water or hot water and its aqueous solution again can be by 4 times of reppd that part of dietary fiber of volume ethanol; It mainly refers to stored substance and secretion in the plant cell, also comprises part microbial polysaccharide and synthetic type polysaccharide such as pectin, melon bean gum, alginate, glucan and fungi polysaccharide; The insolubility dietary fiber is meant not by human body alimentary canal enzymic digestion and water-fast dietary fiber, comprises cellulose, hemicellulose, lignin etc.Dietary fiber has anti-treating constipation and hemorrhoid, colorectal cancer, angiocardiopathy, and the diabetes-alleviating symptom is regulated the enteron aisle effect and alleviated physiological function such as obesity.
How obtaining natural, effective oligosaccharides and dietary fiber is the focus of studying at present, but compound sugar defaecation Research on effect is mainly concentrated on FOS and application study in Animal nutrition thereof both at home and abroad, and it is used as feed addictive.Research to dietary fiber mainly contains extraction preparation from pomace, orange peel, bean dregs, the root of kudzu vine, marine alga etc., and the report of specializing in the edible fiber cathartic function is few.
Banana is the tropical and subtropical zone characteristic fruit, is the fourth-largest fruit after output is only second to apple, oranges and tangerines, pears in China, and it is nutritious; Smell delicate fragrance fragrance is distinguished the flavor of sweet and tasty mouthful, and meat is soft lubricious; Old children is all suitable, and banana promotes enterogastric peristalsis, and defecation, cosmetology function common people generally acknowledge.Chinese scholars is discovered the greater functionality of banana in recent years, as reducing blood lipid, hypotensive, anti-oxidant, improve immunity and alleviate anxiety etc.
Banana is cited as " king of new fruit " in world's fruits and vegetables conference in 2005.The world all is rapid ascendant trend with the banana total output of China, and China's banana gross output had reached 6,900,000 tons in 2006.Banana is to breathe modification fruit more, and storage tolerance is not perishable, and it is serious to adopt back loss ten minutes, and process technology falls behind simultaneously, promptly wastes and contaminated environment.
Meanwhile, the clear and definite material base of banana treatment constipation does not appear in the newspapers, and the mechanism of action it be unclear that, and does not see the research report with banana oligosaccharide and dietary fiber combined preparation bowel relaxing functions food.
Summary of the invention
An object of the present invention is to overcome the deficiency of prior art, a kind of banana oligosaccharide and diet fiber composition are provided.
Another object of the present invention provides said preparation of compositions method.
A further object of the invention provides the application of said composition.
The object of the invention is achieved through following technical scheme:
A kind of banana oligosaccharide and diet fiber composition are provided; Comprise banana oligosaccharide, soluble dietary fiber and insolubility dietary fiber, the mass ratio of said banana oligosaccharide, soluble dietary fiber and insolubility dietary fiber is 2.5: 1: 2 or 2: 2: 2.
The present invention provides the preparation method of above-mentioned banana oligosaccharide and diet fiber composition simultaneously, may further comprise the steps:
(1) banana peeling, with the pulp making beating, the pulp after the flooding making beating, pomace is put in addition, absolute ethyl alcohol deposition extract, centrifugation, collecting precipitation, the vacuum freeze-drying deposition prepares banana oligosaccharide;
Add enzyme in the pomace of (2) putting in addition after step (1) lixiviate and carry out enzymolysis, centrifugalize behind the enzymolysis, isolate the deposition vacuum freeze-drying and get banana insolubility dietary fiber; Supernatant precipitates through absolute ethyl alcohol, vacuum freeze-drying precipitate soluble dietary fiber;
(3) proportionally mix banana oligosaccharide, soluble dietary fiber and insolubility dietary fiber and get said composition.
The said making beating of above-mentioned steps (1) can be adopted common fruit and vegetable pulping machine, and beating time is 2～4min; The feed liquid w/v of said flooding is 1: 1; Extraction temperature is 50～60 ℃, and extraction time is 50～60min, extracts 4 times; Merge No. 4 times extract; With the absolute ethyl alcohol deposition extract of 4 times of extracting liquid volumes, said centrifugation is with the centrifugal 12～14min of 3500～4500 rev/mins rotating speed, and said vacuum freeze-drying is that freeze-drying 4～5h gets banana oligosaccharide under 50Pa～150Pa;
The said enzymolysis of above-mentioned steps (2) comprises two steps:
Steps A: in pomace, add amylase; Diastatic consumption is 3.6～4.0% of a pomace weight; 95～100 ℃ of operative temperatures; Adding calcium chloride adjusting calcium ion concentration is 15～20mmol/L, and using hydrochloric acid or sodium hydroxide solution to regulate the pH value is 4～4.5,95～100 ℃ of water-baths vibration enzymolysis 8～10h.
Step B: in the mixture behind the steps A enzymolysis, add protease, the consumption of protease is 0.20～0.25% of a pomace weight, and 50～55 ℃ of operative temperatures, pH value are 7～7.5, water-bath vibration enzymolysis 2～2.5h.
Centrifugal 12～the 14min of rotating speed that the rotating speed of the said centrifugation of step (2) and time are respectively 3500～4500 rev/mins; The consumption of said absolute ethyl alcohol is 4 times of supernatant volumes; Said vacuum freeze-drying is freeze-drying 6～7h under 80Pa～200Pa condition.
One of preferably preparation scheme of the present invention is with the banana peeling, banana pulp making beating 2.5min, flooding; The feed liquid w/v is 1g: 1ml, and extraction temperature is 60 ℃, and extraction time is 50min; Extract 4 times, merge No. 4 extracts (pomace is put in addition), concentrate through suction filtration and vacuum; With the absolute ethyl alcohol of 4 times of extracting liquid volumes deposition, 3500 rev/mins of centrifugal 14min, collecting precipitation, 50Pa～80Pa vacuum freeze-drying 4h obtains the banana oligosaccharide product;
Residual pomace is used for enzymolysis and prepares the solvable and soluble dietary fiber of banana after the lixiviate banana liquid glucose, and wherein the amylase consumption is 3.6% of a pomace weight, 100 ℃ of operative temperatures, and calcium ion concentration 15mmol/L, the pH value is 4,95 ℃ of water-bath vibration enzymolysis 10h; The protease consumption is 0.2% of a pomace weight, and the pH value is 7,50 ℃ of water-bath vibration enzymolysis 2.5h; Water-bath vibration enzymolysis 2.5h; 3500 rev/mins of rotating speeds centrifugalize 14min down behind the enzymolysis,, the isolated 100Pa of being deposited in～200Pa vacuum freeze-drying 7h gets banana insolubility dietary fiber; Supernatant adds 4 times of volume absolute ethyl alcohols and must precipitate, and is deposited in 100Pa～200Pa vacuum freeze-drying 7h and gets banana soluble dietary fiber finished product;
Compound sugar product and solvable, insolubility dietary fiber finished product are to mix at 2.5: 1: 2 to obtain said composition according to mass ratio.
It is with banana peeling that the present invention preferably prepares two of scheme, with the banana pulp 3min that pulls an oar, and flooding, the feed liquid w/v is 1g: 1ml, and extraction temperature is 50 ℃, and extraction time is 60min, extracts 4 times; Merge No. 4 extracts (pomace is put in addition), concentrate through suction filtration and vacuum, with the absolute ethyl alcohol of 4 times of extracting liquid volumes precipitate, 4500 rev/mins of centrifugal 12min, collecting precipitation, 60Pa～100Pa vacuum freeze-drying 5h obtains the banana oligosaccharide product;
Residual pomace is used for enzymolysis and prepares the solvable and soluble dietary fiber of banana after the lixiviate banana liquid glucose; Wherein the amylase consumption is 4.0% of a pomace weight, 95 ℃ of operative temperatures, calcium ion concentration 20mmol/L; The pH value is 4.5,95～100 ℃ of water-bath vibration enzymolysis 8h; The protease consumption is 0.25% of a pomace weight; 55 ℃ of temperature, pH value are 7.5,55 ℃ of water-bath vibration enzymolysis 2h; 4500 rev/mins of rotating speeds centrifugalize 12min down behind the enzymolysis, and freeze-drying 6h gets banana insolubility dietary fiber under the isolated 80Pa of being deposited in～150Pa vacuum; Adding 4 times of volume absolute ethyl alcohols in the supernatant must precipitate, and is deposited in that freeze-drying 6h gets banana soluble dietary fiber finished product under 80Pa～150Pa vacuum;
Compound sugar product and solvable, insolubility dietary fiber finished product are to mix at 2: 2: 2 to obtain said composition according to portfolio ratio.
Composition according to the invention can be applicable to prepare bowel relaxing functions property food, beverage or is used to prepare the medicine of treating constipation.
The invention has the beneficial effects as follows:
(1) the present invention is the defaecation effect that the banana oligosaccharide that obtains of feedstock production and soluble dietary fiber, insolubility diet fiber composition not only possess common cathartic with the banana; And gentle, do not stimulate; Common rheum officinale, the caused side effect of anthraquinone class cathartic have been overcome; And can bring into play prevention colorectal cancer, the angiocardiopathy of dietary fiber, the diabetes-alleviating symptom is regulated physiological functions such as enteron aisle effect and fat-reducing.Because dietary fiber of the present invention has stronger retention ability, combines waterpower; Can promote moisture to shift, make ight soil have bigger volume and higher wettability, reach the purpose that water holding is lubricated, keep height to ooze to enteron aisle and intestinal contents; Dietary fiber of the present invention is from banana; Thing experimental result combined according to the invention, said composition is safe and effective, especially is fit to alleviate the infant because the constipation symptom that the close knot of being hard and dry that edible milk powder causes is difficult to resolve.
(2) the present invention provides technical support for the clear and definite material base and the mechanism of action of research banana treatment constipation, and can effectively solve the pressure of banana fresh-keeping, processing.
(3) the present invention all effectively utilizes banana pulp, kept the nutritional labeling of banana, and the preparation method is simple, and the preparation process is simple and easy, and cost is lower.
The specific embodiment
Come further explain the present invention below in conjunction with specific embodiment.
Embodiment 1 prepares composition according to following method
(1) banana peeling; Pulp making beating 2.5min, flooding, the feed liquid w/v is 1g: 1ml, and temperature is 60 ℃, and extraction time is 50min, extracts 4 times; Merge No. 4 times extract, pomace is put in addition, and extract concentrates through suction filtration and vacuum, with the absolute ethyl alcohol of 4 times of extracting liquid volumes precipitate, 3500 rev/mins of centrifugal 14min, collecting precipitation, 50Pa～80Pa vacuum freeze-drying 4h obtains the banana oligosaccharide product;
(2) residual pomace is used for enzymolysis and prepares the solvable and soluble dietary fiber of banana after the lixiviate banana liquid glucose:
Steps A: in pomace, add amylase; Diastatic consumption is 3.6% of a pomace weight, 100 ℃ of operative temperatures, and it is 15mmol/L that adding calcium chloride makes calcium ion concentration; Through adding hydrochloric acid and sodium hydroxide solution adjusting pH value is 4,95～100 ℃ of water-baths vibration enzymolysis 10h;
Step B: in the steps A enzymolysis liquid, add protease, the consumption of protease is 0.2% of a pomace weight, 50 ℃ of temperature, and adding hydrochloric acid and sodium hydroxide solution adjusting pH value is 7,50 ℃ of water-baths vibration enzymolysis 2.5h;
At the centrifugal 12～14min of 3500～4500 rev/mins rotating speed, freeze-drying 7h gets banana insolubility dietary fiber under the isolated 100Pa of being deposited in～200Pa vacuum behind the enzymolysis; The absolute ethyl alcohol that adds 4 times of supernatant volumes in the supernatant must precipitate, and is deposited in that freeze-drying 7h gets banana soluble dietary fiber finished product under 100Pa～200Pa vacuum;
(3) compound sugar product and solvable, insolubility dietary fiber finished product are to mix at 2.5: 1: 2 to obtain said composition 1 according to mass ratio.
Embodiment 2
(1) banana peeling; Pulp making beating 3min, flooding, the feed liquid w/v is 1g: 1ml, and temperature is 50 ℃, and extraction time is 60min, extracts 4 times; Merge No. 4 times extract, pomace is put in addition, extract through conventional suction filtration and vacuum concentrate the back with the absolute ethyl alcohol of 4 times of extracting liquid volumes precipitate, 4500 rev/mins of centrifugal 12min, collecting precipitation, 60Pa～100Pa vacuum freeze-drying 5h obtains the banana oligosaccharide product;
(2) residual pomace is used for enzymolysis and prepares the solvable and soluble dietary fiber of banana after the lixiviate banana liquid glucose:
Steps A: in pomace, add amylase; Diastatic consumption is 4.0% of a pomace weight, 95 ℃ of operative temperatures, and it is 20mmol/L that adding calcium chloride makes calcium ion concentration; Through adding hydrochloric acid and sodium hydroxide solution adjusting pH value is 4.5,100 ℃ of water-baths vibration enzymolysis 8h;
Step B: in the steps A enzymolysis liquid, add protease, the consumption of protease is 0.25% of a pomace weight, and 55 ℃ of temperature are 7.5,50 ℃ of water-baths vibration enzymolysis 2.2h through adding hydrochloric acid and sodium hydroxide solution adjusting pH value;
At the centrifugal 14min of 3500 rev/mins rotating speed, freeze-drying 6h gets banana insolubility dietary fiber under the isolated 80Pa of being deposited in～150Pa vacuum behind the enzymolysis; The absolute ethyl alcohol that adds 4 times of supernatant volumes in the supernatant must precipitate, and is deposited in that freeze-drying 6h gets banana soluble dietary fiber finished product under 80Pa～150Pa vacuum;
(3) compound sugar product and solvable, insolubility dietary fiber finished product are to mix at 2: 2: 2 to obtain said composition 2 according to portfolio ratio.
Embodiment 3
(1) banana peeling; Pulp making beating 2min, flooding, the feed liquid w/v is 1g: 1ml, and temperature is 55 ℃, and extraction time is 55min, extracts 5 times; Merge No. 5 times extract, pomace is put in addition, and extract concentrates through suction filtration and vacuum, with the absolute ethyl alcohol of 4 times of extracting liquid volumes precipitate, 4000 rev/mins of centrifugal 13min, collecting precipitation, 100Pa～150Pa vacuum freeze-drying 4h obtains the banana oligosaccharide product;
(2) residual pomace is used for enzymolysis and prepares the solvable and soluble dietary fiber of banana after the lixiviate banana liquid glucose:
Steps A: in pomace, add amylase; Diastatic consumption is 3.8% of a pomace weight, 98 ℃ of operative temperatures, and it is 18mmol/L that adding calcium chloride makes calcium ion concentration; Through adding hydrochloric acid and sodium hydroxide solution adjusting pH value is 4.2,100 ℃ of water-baths vibration enzymolysis 9h;
Step B: in the steps A enzymolysis liquid, add protease, the consumption of protease is 0.22%, and 53 ℃ of temperature are 7.2,55 ℃ of water-baths vibration enzymolysis 2h through adding hydrochloric acid and sodium hydroxide solution adjusting pH value;
At the centrifugal 12min of 4500 rev/mins rotating speed, freeze-drying 6h gets banana insolubility dietary fiber under the isolated 80Pa of being deposited in～150Pa vacuum behind the enzymolysis; The absolute ethyl alcohol that adds 4 times of supernatant volumes in the supernatant must precipitate, and is deposited in that freeze-drying 6.5h gets banana soluble dietary fiber finished product under 90Pa～120Pa vacuum;
(3) compound sugar product and solvable, insolubility dietary fiber finished product are 2: 2: 2 according to mass ratio, mix obtaining said composition 3.
Embodiment 4 zooperies
Experimental technique is with reference to " health food check and assessment technique enforcement of regulations handbook (publishing in 2003), " the bowel relaxing functions method of inspection ".Animal: SPF level Kunming mouse, male, purchase in Guangdong Medical Lab Animal Center animal credit number SCXK 2003-0002,2007A006.
Experimental result is seen shown in the table 1:
The influence of table 1 pair small intestine prepared Chinese ink propelling rate
A)
| Group | Dosage g/ (kgBWd) | Prepared Chinese ink propelling rate P (%) |
| The blank group | 0 | 76.6±5.8 * |
| Compound diphenoxylate constipation model control group | 0.005 | 44.7±5.4 |
| Simple banana oligosaccharide product | 2.5 | 70.8±8.0 * |
| Simple banana soluble dietary fiber finished product | 1.0 | 50.9±12.0 |
| Simple banana soluble dietary fiber finished product | 2.0 | 62.6±11.8 * |
| Simple banana insolubility dietary fiber finished product | 2.0 | 54.7±15.2 |
| Composition 1 | Compound sugar 2.5 solvable dietary fiber 1 insolubility dietary fibers 2 | 60.2±4.4 * |
| Composition 2 | Compound sugar 2 | 59.2±7.3 * |
| Solvable dietary fiber 2 insolubility dietary fibers 2 |
A) compare with the constipation model control group
*P＜0.05.
Zoopery shows, under present composition effect, can make mouse small intestine prepared Chinese ink propelling rate reach 60.2%～66.5%, is significantly higher than the propelling rate 44.7% of compound diphenoxylate constipation model control group.
Claims (6)
1. banana oligosaccharide and diet fiber composition; It is characterized in that comprising banana oligosaccharide, soluble dietary fiber and insolubility dietary fiber, said banana oligosaccharide, soluble dietary fiber and insolubility dietary fiber quality ratio are 2.5: 1: 2 or 2: 2: 2;
Said preparation of compositions method may further comprise the steps:
(1) banana pulp making beating, flooding, pomace is put in addition, precipitates extract with absolute ethyl alcohol, collecting precipitation, the vacuum freeze-drying deposition prepares banana oligosaccharide;
Add enzyme in the pomace of (2) putting in addition after step (1) lixiviate and carry out enzymolysis, centrifugalize behind the enzymolysis, deposition gets banana insolubility dietary fiber through vacuum freeze-drying; Supernatant precipitates through absolute ethyl alcohol, vacuum freeze-drying precipitate soluble dietary fiber;
(3) proportionally mix banana oligosaccharide, soluble dietary fiber and insolubility dietary fiber and get said composition;
Wherein, the said beating time of step (1) is 2～4min; The feed liquid w/v of said flooding is 1g: 1ml, and extraction temperature is 50～60 ℃, and extraction time is 50～60min; The consumption of absolute ethyl alcohol is 4 times of extracting liquid volumes; Said vacuum freeze-drying is freeze-drying 4～5h under 50Pa～150Pa;
The said enzymolysis of step (2) comprises two steps:
Steps A: in pomace, add amylase, diastatic consumption is 3.6～4.0% of a pomace weight, and 95～100 ℃ of operative temperatures are regulated calcium ion concentration 15～20mmol/L, and the pH value is 4～4.5, water-bath vibration enzymolysis 8～10h;
Step B: in the mixture behind the steps A enzymolysis, add protease, the consumption of protease is 0.20～0.25% of a pomace weight, and 50～55 ℃ of operative temperatures, pH value are 7～7.5, water-bath vibration enzymolysis 2～2.5h.
2. composition according to claim 1 is characterized in that step (A) adds calcium chloride and regulates calcium ion concentration.
3. composition according to claim 1, the consumption that it is characterized in that the said absolute ethyl alcohol of step (2) is 4 times of supernatant volumes; Said vacuum freeze-drying is freeze-drying 6～7h under 80Pa～200Pa condition.
4. composition according to claim 1; It is characterized in that it being with the banana pulp 2.5min that pulls an oar, is that 1g: 1ml adds 60 ℃ of following lixiviate 50min of entry according to the feed liquid w/v, extracts 4 times; Merge No. 4 times extract; Concentrate the back through suction filtration and vacuum and precipitate with the absolute ethyl alcohol of 4 times of extracting liquid volumes, centrifugal collecting precipitation afterwards, 50Pa～80Pa vacuum freeze-drying 4h obtains the banana oligosaccharide product;
3.6% amylase, the calcium ion of 15mmol/L, adjust pH that pomace after the lixiviate adds pomace weight are 4,95 ℃ of water-baths vibration enzymolysis 10h; 0.2% protease, the adjust pH that add pomace weight then are 7; 50 ℃ of water-bath vibration enzymolysis 2.5h; Centrifugalize behind the enzymolysis, be deposited in 100Pa～200Pa vacuum freeze-drying 7h and get banana insolubility dietary fiber; Supernatant adds 4 times of volume absolute ethyl alcohols and must precipitate, and is deposited in 100Pa～200Pa vacuum freeze-drying 7h and gets banana soluble dietary fiber finished product;
Is to mix at 2.5: 1: 2 to obtain said composition with compound sugar and solvable, insolubility dietary fiber according to portfolio ratio.
5. composition according to claim 1 is characterized in that it being with the banana pulp 3min that pulls an oar, and is that 1g: 1ml adds 60 ℃ of following lixiviate 50min of entry according to the feed liquid w/v, extracts 4 times; Merge No. 4 times extract, concentrate the back through suction filtration and vacuum and precipitate with the absolute ethyl alcohol of 4 times of extracting liquid volumes, centrifugal collecting precipitation afterwards, 50Pa～80Pa vacuum freeze-drying 4h obtains the banana oligosaccharide product;
4.0% amylase, the calcium ion of 20mmol/L, adjust pH that pomace after the lixiviate adds pomace weight are 4.5,100 ℃ of water-baths vibration enzymolysis 8h; 0.25% protease, the adjust pH that add pomace weight then are 7.5; 55 ℃ of water-bath vibration enzymolysis 2h; Centrifugalize behind the enzymolysis, be deposited in 80Pa～150Pa vacuum freeze-drying 6h and get banana insolubility dietary fiber; Supernatant adds 4 times of volume absolute ethyl alcohols and must precipitate, and is deposited in 80Pa～150Pa vacuum freeze-drying 6h and gets banana soluble dietary fiber finished product;
Is to mix at 2: 2: 2 to obtain said composition with compound sugar and solvable, insolubility dietary fiber according to portfolio ratio.
6. the application of the said composition of claim 1 is characterized in that being applied to prepare bowel relaxing functions property food, beverage or be used to prepare the medicine aspect of treating constipation.
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| CN102640935A (en) * | 2012-05-24 | 2012-08-22 | 中华全国供销合作总社济南果品研究院 | Method for extracting dietary fiber in banana skin by adopting enzymic method |
| CN103355612B (en) * | 2013-07-18 | 2014-09-10 | 华南理工大学 | Making method of banana oligosaccharide-resistant starch combined food |
| CN105146271B (en) * | 2015-09-22 | 2018-10-16 | 天蕉健康食品（广东）有限公司 | A kind of production method of banana resistant starch and dietary fiber composition food with weight losing function |
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