CN111387394A - Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof - Google Patents

Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof Download PDF

Info

Publication number
CN111387394A
CN111387394A CN201910004101.9A CN201910004101A CN111387394A CN 111387394 A CN111387394 A CN 111387394A CN 201910004101 A CN201910004101 A CN 201910004101A CN 111387394 A CN111387394 A CN 111387394A
Authority
CN
China
Prior art keywords
powder
parts
sea
solid beverage
buckthorn
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910004101.9A
Other languages
Chinese (zh)
Inventor
李志霞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanxi Xishengyuan Science And Trade Co ltd
Original Assignee
Shanxi Xishengyuan Science And Trade Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanxi Xishengyuan Science And Trade Co ltd filed Critical Shanxi Xishengyuan Science And Trade Co ltd
Priority to CN201910004101.9A priority Critical patent/CN111387394A/en
Publication of CN111387394A publication Critical patent/CN111387394A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/385—Concentrates of non-alcoholic beverages
    • A23L2/39—Dry compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12—Fatty acids or derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention provides a sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and a preparation method thereof, wherein the sea-buckthorn solid beverage comprises, by weight, 3000 parts of sea-buckthorn fruit powder, 1800 parts of maltodextrin 1200, 700 parts of pumpkin powder, 400 parts of soybean peptide powder, 400 parts of pineapple powder, 200 parts of yeast β -glucan 100, 300 parts of cranberry fruit powder 100, 300 parts of konjac powder 100, 60-180 parts of lutein ester microcapsule powder, 50-150 parts of Plantago ovata forsk shell powder, 20-40 parts of Momordica grosvenori powder, 300-15 parts of vitamin C5-15 parts, 10-50 parts of xylitol, 10-50 parts of citric acid and 2-8 parts of sucralose.

Description

Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof
Technical Field
The invention relates to the technical field of pure natural plant sea-buckthorn solid beverages, in particular to a sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and a preparation method thereof.
Background
The immunity is a defense mechanism of a human body, is the capacity of the human body to recognize and eliminate any foreign matters (viruses, bacteria and the like) invaded from the outside, treat aged, damaged, dead and denatured self cells, recognize and treat in-vivo mutant cells and virus infected cells, and is a physiological response of the human body to recognize and eliminate 'isohexia'.
The fructus Hippophae powder contains natural vitamin C, protein, amino acids, vitamin E, vitamin K, fructus Hippophae polysaccharide, vitamin B1, vitamin B2, vitamin B6, fructus Hippophae flavone, vitamin P, carotenoid, phytosterol, phospholipid, unsaturated fatty acid, organic acid, carbohydrate and microelements. Sea-buckthorn is rich in vitamin A, B1, B2, C, D, E, folic acid, flavone and the like, and the Vc content of the sea-buckthorn is far higher than that of fresh jujube called Vc king and kiwi fruit called Vc king, so that the sea-buckthorn is known as natural vitamin treasury. The sea buckthorn has rich vitamin C, can resist fatigue, promote hemopoiesis, protect digestive tract, eliminate free radical, improve blood vessel system, strengthen immunity and prevent ailment. The sea buckthorn also has high medicinal value, can reduce cholesterol, relieve angina attack and has the effect of preventing and treating coronary atherosclerotic heart disease; has expectorant, antitussive, antiasthmatic, and chronic tracheitis treating effects; can be used for treating gastric ulcer, duodenal ulcer, dyspepsia, etc., and has remarkable therapeutic effect on chronic superficial gastritis, atrophic gastritis, colitis, etc.; has good therapeutic effect on burn, scald, incised wound and cold injury; has good therapeutic effect on cervical erosion. The fructus Hippophae powder can also protect our liver, prevent liver cirrhosis, prevent and treat cardiovascular and cerebrovascular diseases, and prevent tumor and cancer. The fructus Hippophae powder can prevent and treat atherosclerosis, reduce low density lipoprotein, increase high density lipoprotein, clean the deposit in blood vessel wall, and promote metabolism. The properties of seabuckthorn fruits recorded in the Chinese traditional medicine dictionary are as follows: warm in nature, sour and astringent in taste, enters the channels of lung, stomach, large intestine and small intestine, and has the effects of promoting blood circulation, removing blood stasis, eliminating phlegm, relieving chest stuffiness, invigorating spleen and stomach, promoting fluid production, quenching thirst, clearing heat, relieving diarrhea, etc. This is symptomatic for the physical discomfort caused by various viruses. The common traditional Chinese medicine has the advantages of little damage to human tissue cells due to virus resistance and small toxic and side effects.
The yeast glucan is a polysaccharide which exists in yeast cell walls and has the function of enhancing the immunity, β -glucan, β -glucan is a polysaccharide which exists in various fungi and plants, such as mushrooms, lucid ganoderma and oats, and is a main functional substance which plays a role in health care, the yeast glucan is a main component of the yeast cell walls, and is a natural and safe polysaccharide which is completely extracted from yeast.
The immunopotentiator is mainly used for enhancing the anti-tumor and anti-infection capability of the body and correcting immunodeficiency. The medicine can activate one or more immune active cells, enhance nonspecific and specific immune function of organism, restore low immune function to normal, or play adjuvant role to enhance immunogenicity of combined antigen, and accelerate induction of immune response reaction; or replace the lack of immune active components in vivo, play a role in immune replacement; or has bidirectional regulating effect on the immune function of the organism, so that the over-high or over-low immune function tends to be normal. The medicine is mainly used for immunodeficiency diseases, malignant tumors and refractory bacterial or viral infection in clinic.
The currently clinically commonly used immunopotentiator comprises chemical synthesis medicines such as levamisole, isoprinosine and the like, human or animal immune products such as thymosin, transfer factor, interferon, interleukin and the like, bacillus calmette-guerin from microorganisms and biological polysaccharide from other sources, traditional Chinese medicine active ingredients and the like, wherein the function mechanism of the medicines for regulating the immune function comprises ① enhancing the activity of macrophages and natural killer cells and improving the nonspecific immune function, ② promoting the division, proliferation, maturation and differentiation of T lymphocytes and enhancing the cellular immune function, ③ improving the humoral immune function, ④ inducing the generation of interferon and certain cell factors and activating immune cells to play a role.
At present, most of western medicines for enhancing immunity and inhibiting tumors on the market are poor in taste and high in price, and have adverse reactions after long-term use. Levamisole, for example, can cause gastrointestinal reactions such as nausea, vomiting, abdominal pain, and the like; BCG vaccine has more adverse reactions, and can cause skin redness, swelling, induration and ulcer at local part of injection, and occasional anaphylactic shock and even death. Interferon can cause flu-like symptoms, and some patients can also have symptoms such as bone marrow suppression, arrhythmia, confusion, hallucinations, and the like. The patients who take the medicine can have serious physical and psychological dependence on the medicine, and the medicine is not suitable for long-term taking.
The existing traditional Chinese medicine preparation can also treat the symptoms, but has the problems of large dosage, slow effect, low titer and the like. Some traditional Chinese medicine preparations have definite curative effect and good economic benefit, but if the traditional Chinese medicine preparations are taken too much carelessly, adverse reaction can be caused.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects in the prior art, so that the sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and the preparation method thereof are provided. In order to achieve the purpose, the invention adopts the following technical scheme:
a sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following raw materials in parts by weight:
3000 parts of sea buckthorn fruit powder, 1800 parts of maltodextrin 1200, 700 parts of pumpkin powder, 400 parts of soybean peptide powder, 400 parts of pineapple powder, 300 parts of yeast β -glucan 100, 300 parts of cranberry fruit powder, 300 parts of konjac powder, 60-180 parts of lutein ester microcapsule powder, 50-150 parts of Plantago ovata husk powder, 20-40 parts of Hance fruit powder, 5-15 parts of vitamin C, 10-50 parts of xylitol, 10-50 parts of citric acid and 2-8 parts of sucralose.
Preferably: a sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following raw materials in parts by weight:
2200-2800 parts of sea buckthorn fruit powder, 1300-1700 parts of maltodextrin, 320-600 parts of pumpkin powder, 220-380 parts of soybean peptide powder, 220-380 parts of pineapple powder, β -280 parts of glucan, 120-280 parts of cranberry powder, 120-280 parts of konjac powder, 80-160 parts of lutein ester microcapsule powder, 80-120 parts of Plantago ovata husk powder, 22-38 parts of Momordica grosvenori powder, 6.8-14.8 parts of vitamin C, 12-40 parts of xylitol, 12-40 parts of citric acid and 2.2-7.8 parts of sucralose.
Preferably, the sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following raw materials, by weight, 2500 parts of sea-buckthorn fruit powder, 1500 parts of maltodextrin, 500 parts of pumpkin powder, 300 parts of soybean peptide powder, 300 parts of pineapple powder, 200 parts of yeast β -glucan, 200 parts of cranberry fruit powder, 200 parts of konjac powder, 120 parts of lutein ester microcapsule powder, 100 parts of Plantago ovata husk powder, 30 parts of fructus momordicae powder, 10 parts of vitamin C, 30 parts of xylitol, 15 parts of citric acid and 5 parts of sucralose.
Preferably: a sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors further comprises 10 parts by weight of vitamin C.
A method for preparing the sea buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following steps:
(1) grinding and sieving the raw materials respectively to obtain fructus Hippophae powder, maltodextrin, fructus Cucurbitae Moschatae powder, soybean peptide powder, fructus Ananadis Comosi powder, yeast β -dextran, cranberry fruit powder, rhizoma Amorphophalli powder, lutein ester microcapsule powder, Plantago ovata seed husk powder, fructus Siraitiae Grosvenorii powder, vitamin C, xylitol, citric acid and sucralose powder for use;
(2) weighing the momordica grosvenori powder, the vitamin C, the xylitol and the citric acid in parts by weight, and mixing to obtain a first mixed material; mixing and stirring the first mixed material and the sucralose powder in an equivalent increasing mode to obtain a second mixed material;
(3) and uniformly mixing the second mixed material with the seabuckthorn fruit powder, maltodextrin, pumpkin powder, soybean peptide powder, pineapple powder, yeast β -glucan, cranberry fruit powder, konjak powder, lutein ester microcapsule powder and Plantago ovata seed husk powder to obtain the seabuckthorn solid beverage.
Preferably, the particle sizes of the seabuckthorn fruit powder, the maltodextrin, the pumpkin powder, the soybean peptide powder, the pineapple powder, the yeast β -glucan, the cranberry fruit powder, the konjac powder, the lutein ester microcapsule powder, the Plantago ovata seed husk powder, the Momordica grosvenori powder, the vitamin C, the xylitol, the citric acid and the sucralose powder in the step (1) are not more than 178 microns.
Preferably: the mixing time in the step (2) is 28-32 minutes, and the mixing and stirring time is 28-32 minutes.
Further: the mixing time in the step (2) is 30 minutes, and the mixing and stirring time is 30 minutes.
The components are mixed by adopting an equivalent increasing method according to the method, the mixing effect is optimal when the mixing time and the mixing and stirring time are controlled to be 30 minutes, and the efficiency is highest.
Preferably: the time for uniform mixing in the step (3) is 110-130 minutes.
Further: the time for uniformly mixing in the step (3) is 2 hours.
Maltodextrins are also known as water soluble dextrins or enzymatic dextrins. The maltodextrin series products produced in various places of China are produced by using corn, rice and the like as direct raw materials and adopting an enzyme method process. The maltodextrin is white powder or granule, slightly absorbs water, has no sweet taste or slight sweet taste, and has nutritive value; is easily soluble in water or easily dispersible in water, and can be clear to turbid water solution. The maltodextrin has the characteristics of strong thickening property, good carrier property, small fermentability, good filling effect, no moisture absorption, no peculiar smell, easy digestion, low heat, low sweetness and the like. The maltodextrin contains abundant polysaccharides, trace elements and minerals such as calcium and iron beneficial to human body, and can promote normal metabolism of human body. Is widely applied to the industries of beverages, frozen foods, candies, oatmeal, dairy products, health care products and the like, and can also be applied to the production of textiles, daily chemicals and medicines.
The pumpkin powder is prepared from pumpkin, namely rice melon, pumpkin, and the like, and belongs to cucurbitaceae annual herbaceous plants, and contains abundant carbohydrates, proteins, dietary fibers, vitamins, carotene, pectin, abundant trace elements such as potassium, iron, magnesium and the like, in addition, citrulline, arginine and the like, and enzymes are very beneficial to a human body, and the fat content is very low. Experts believe that pumpkins span six steps across the food service: staple food-vegetables and fruits-foreign food-beverage health food-pharmacological food. People pay attention to their pumpkin diet with new feelings and sights. The pumpkin powder is golden yellow in color, has fresh pumpkin feeling in the mouth, is easy to dissolve in water, has the mesh number adjustable between 100 meshes and 120 meshes, does not contain any additive, preservative and pigment, and does not contain substances similar or similar in color and taste, thereby being a pure natural green food which ensures 100 percent. But also has the functions of relaxing muscles and tendons, promoting blood circulation, simulating skin chapping, preventing cancer and the like, is beneficial to human body development, enhances the immunity in human body and the like.
The soybean peptide powder is a mixed peptide product consisting of 2-6 amino acids. The soybean peptide powder has balanced and comprehensive amino acid composition and contains 9 essential amino acids. Compared with the original protein and amino acid preparation with the same composition, the soybean peptide powder has good water solubility, water retention and foamability, can be completely dissolved under any acid-base condition with acidity (pH) of 2-10, can be completely dissolved with other food ingredients, and keeps the respective original physicochemical and nutritional characteristics. It is easy to be absorbed by human body, and can be directly absorbed by small intestine without gastrointestinal digestion, and is not affected by physical condition. Besides rich nutrition and easy absorption, the health-care food has the effects of resisting hypertension, reducing cholesterol, resisting thrombosis, improving lipid metabolism, preventing arteriosclerosis, enhancing physical strength and muscle strength of a human body, promoting recovery of myoerythrocytes, eliminating fatigue, promoting absorption of calcium, phosphorus and other trace elements, can form soybean peptide complex calcium which directly enters blood and is deposited in bones without participation of vitamin D, and achieves the effect of improving the strength of bones; the peptide can also promote brain development, improve memory, enhance the activity of phagocyte and B cell, enhance immunologic function, resist cancer and aging, protect epidermal cell, prevent melanin pigmentation, eliminate oxidation free radical, and play a role of blood scavenger; can promote cell reproduction, protect liver, prevent liver cirrhosis, and promote liver function recovery, and has medical and prophylactic effects for diabetes patients; and can be used for preventing senile dementia and cholelithiasis. As a novel multifunctional nutritional ingredient, the soybean peptide powder can be widely used in health food, infant food, sports food, fermented products, clinical nutritional preparations and the like.
The yeast β -glucan is a new resource food, takes saccharomyces cerevisiae as a raw material, and is produced by the steps of extraction, acid-base treatment, spray drying and the like, β -glucan has an active structure which is polysaccharide consisting of glucose units, most of the polysaccharide is combined through β -1, 3, which is a mode of glucose chain connection, and can activate macrophages, neutrophilic leukocytes and the like, so that the content of leukocytins, cytokinins and special antibodies can be improved, and the glucan is widely present in various fungi and plants, such as mushrooms, lucid ganoderma and oats, and is a main functional substance which can play a health care role.
Cranberries are also known as cranberries. The cranberry juice can effectively inhibit helicobacter pylori, resist bacterial gastric ulcer, has strong antioxidation, can reduce low-density cholesterol and triglyceride, can inhibit growth and reproduction of various pathogenic bacteria, prevent the pathogenic bacteria from adhering to in-vivo cells (such as epithelial cells of the urinary tract), prevent and control urinary tract infection and inhibit helicobacter pylori infection. The cranberry contains vitamin C, flavonoid and other antioxidant substances and abundant pectin, and has effects of caring skin, improving constipation, and promoting discharge of toxin and excessive fat.
Konjak is a perennial herb rhizome plant of the family arisaematis. Konjak is a family of people, has about hundreds of species all over the world, has more than 30 species in China, and has a wide planting area in China. The konjak has wide application, and the konjak is prepared into konjak fine powder through research for over ten years. There are hundreds of food products made from konjak. Konjak is the only commercial crop which is found at present and can provide a large amount of glucan, and the use value of konjak is constantly developed and displayed in the field of food and medicine. The edible value of konjak makes it popular with people, the main component of konjak is glucomannan, which not only contains more than 10 amino acids and several trace elements necessary for human body, but also has the characteristics of low protein, low fat and high fiber, good water absorption and high expansion rate, etc., and its medicinal effect has the several effects of reducing blood fat, reducing blood sugar and blood pressure, reducing weight, beautifying face, protecting health and relaxing bowels. The unique syneresis hydrogel fiber of konjak can promote the rapid elimination of heavy metal elements and food toxic substances in the intestinal tract, reduce the contact time of harmful substances with the intestinal wall, prevent the absorption of the substances by the intestinal tract, clear the retentates in the stomach, the intestine and the gall bladder, effectively reduce the probability of harmful bacterial infection in the colon and the rectum, and is ideal cancer prevention health food.
Seabuckthorn is a deciduous shrub which is drought-resistant and sand-resistant and can survive in saline-alkali lands, so that seabuckthorn is widely used for water and soil conservation. Sea buckthorn is planted in great amount in northwest China for desert greening. Seabuckthorn is a medicinal food containing various vitamins, various trace elements, various amino acids and other bioactive substances. The sea buckthorn fruit has high vitamin C content, and the vitamin is the name of vitamin C king. Sea buckthorn is a generic term for plants and their fruits. The plant Hippophae rhamnoides is Hippophae of Elaeagnaceae, is deciduous shrub, and is distributed in North China, northwest China, southwest China, etc. The fructus Hippophae is a medicinal and edible plant. The root, stem, leaf, flower and fruit of the sea buckthorn, especially the sea buckthorn fruit, contain rich nutrient substances and bioactive substances, and can be widely applied to many fields of food, medicine, light industry, spaceflight, farming and animal husbandry fish industry and other national economy. The fructus Hippophae has effects of relieving cough, eliminating phlegm, invigorating stomach, resolving food stagnation, promoting blood circulation and removing blood stasis. Modern medical research shows that the seabuckthorn fruit powder can protect the liver of people, prevent liver cirrhosis, prevent and treat cardiovascular and cerebrovascular diseases and prevent tumors and cancers; can also clean the sediments in the vessel wall, promote metabolism, improve the immunity of the human body, dredge the vessel and get through microcirculation.
The pineapple is neutral in nature, sweet in taste, slightly sour, slightly astringent and slightly cold in nature, has the effects of clearing summer heat, quenching thirst, helping digestion, stopping diarrhea, tonifying spleen and stomach, strengthening vitality, tonifying qi and blood, helping digestion, eliminating dampness, beautifying and slimming, and enhancing immunity, is rich in vitamin C, and also contains a large amount of nutritional ingredients such as fructose, glucose, citric acid, protein and the like, so that the pineapple is prevented from chapping skin, moisturizes the hair to be bright, and can eliminate the body tension and enhance the immunity of the body. The pineapple peel can be eaten for a long time, the incidence of diabetes mellitus is obviously reduced, and the pineapple peel has certain tumor inhibiting and anticancer effects.
Lutein ester is an important carotenoid fatty acid ester, and the characters are as follows: dark reddish brown fine particles. The high-purity lutein ester (with the content of 60-95%) is golden yellow ester orange red powder, is easily soluble in chlorocarbons such as chloroform, dichloromethane, carbon dichloride and the like, is soluble in solvents such as normal hexane, acetone, ethyl acetate, ethanol and the like, has stronger stability than lutein, and has larger damage to the lutein ester only under the factors of high temperature, strong acid, iron ions, oxygen and the like. The importance of lutein is well established in the world of nutrition; the yellow color of the macula of the human eye is due to its filling with lutein and its congeners. Lutein filters blue light and has antioxidant effect, and is a key nutrient element for promoting eye development. Therefore, some have also compared lutein to "invisible sunglasses". Recent studies have found that supplementation of lutein in vivo at the overall level of animals can promote antigen-stimulated lymphocyte proliferation responses and can affect functional expression of cell surface molecules. The regulation effect of lutein on cellular immunity is mainly reflected in the relationship with apoptosis and gene regulation.
Plantago ovata forsk is the shell of artificially planted plantago ovata forsk seeds of Plantaginaceae, and is widely applied to medicines, health products, foods, beverages and feed products due to the rich soluble dietary fiber. The seed shell of Plantago ovata forsk is also called fiber king, and the fiber in Plantago ovata forsk is a pure natural plant fiber source, can absorb water and expand by about 50 times to form jelly-like sticky material, so it is also called land jelly king. Plantago ovata forsk husk is rich in colloid, and comprises arabinose, xylose, galacturonic acid, semi-dried fatty oil and a small amount of Coralline glycoside. Plantago ovata husk is rich in dietary fiber, and the content of dietary fiber is as high as 80%, and the ratio of soluble dietary fiber to insoluble dietary fiber is as high as 7: 3. Other nutritional components mainly include glucoside, protein, polysaccharide, vitamin B1 and choline. It has been shown by conventional therapy that psyllium seed is used to treat constipation, diarrhea, hemorrhoids, ulcers, bladder disorders, and hypertension. Plantago ovata forsk is also commonly used to combat skin irritation, including poison reactions of ivy and insect bites. In 2014, Plantago ovata forsk was approved by the national Wei Ji Commission as a new food material.
The momordica grosvenori is also known as the fruits of Laohidra, false balsam pear, momordica glabra, Stephania delavayi, Japanese momordica, and nude tortoise, is known as the fruit of Hydnocarpus, is sweet, sour and cool in nature, has the effects of clearing heat and cooling blood, promoting the production of body fluid and relieving cough, smoothing intestine and expelling toxin, tendering skin and benefiting face, moistening lung and reducing phlegm, and can be used for prolonging life, retaining youthful looks and pleasing colors, and treating phlegm-heat cough, sore throat, constipation, and diabetes and dysphoria. The momordica grosvenori is a famous and precious traditional Chinese medicine for both medicine and food, which is published by Ministry of health, contains momordica grosvenori glycoside which is 300 times sweeter than sucrose, does not generate heat, is a famous and precious raw material in beverage and candy industries, and is an optimal substitute for sucrose. It has obvious curative effect on bronchitis, hypertension and other diseases, and also has the functions of preventing and treating coronary heart disease, angiosclerosis and obesity.
Vitamin C, also called L-ascorbic acid, is a water-soluble vitamin, the main function of vitamin C is to improve immunity, such as 1, the white blood cells contain abundant VC, when the organism is infected, the VC in the white blood cells is sharply reduced, the VC can enhance the chemotaxis and deformability of the neutrophilic granulocyte, improve the bactericidal ability 2, promote the generation of lymphoblast, improve the recognition and killing of the organism to the external and malignant cells 3, participate in the synthesis of immunoglobulin 4, promote the generation of interferon, interfere the transcription of virus mRNA, and inhibit the proliferation of virus.
Xylitol is a natural sweetener extracted from plant materials such as white birch, oak, corncob, bagasse and the like, commercial xylitol is prepared by deep processing agricultural crops such as corncob, bagasse and the like, is a natural and healthy sweetener, is named as xylose in Chinese, and is also called as 1, 2, 3, 4, 5-pentanol, and can be used as a sweetener and a wetting agent.
Citric acid is an important organic acid, also known as citric acid, a colorless crystal or white powder having a palatable sour taste; widely found in plants (such as lemon fruit), extracted from lemon and lime juice, or fermented from sugar, and used as flavoring agent for food, carbonated alcoholic beverage, and medicine.
Sucralose is also known as sucralose, saccharin, sucrose crystals, sucralose or 4, 1, 6, -trichloro-4, 1, 6, -trideoxy semimilk sucrose. Sucralose is prepared by chlorination of sucrose as a raw material. Sucralose has the characteristics of no energy, high sweetness, pure sweetness, high safety and the like.
The invention has the beneficial effects that:
(1) the sea-buckthorn solid beverage is used for enhancing immunity and inhibiting tumors, and has the pharmacology that firstly, sea-buckthorn fruit powder, yeast β -glucan, pumpkin powder, soybean peptide powder, pineapple powder, lutein ester microcapsule powder and cranberry fruit powder in the formula have the effects of enhancing immunity and inhibiting tumors, secondly, vitamin C is added to achieve a more remarkable effect of enhancing immunity, the momordica grosvenori powder clears the throat and relieves bronchus pharyngolaryngitis, the sea-buckthorn solid beverage can effectively prevent drug resistance of the product and enhance the immune function by being matched with precious nutritional ingredients such as cranberry fruit powder and Plantago ovata seed husk powder, and the like, and the sea-buckthorn solid beverage can improve the immunity and repair capability of an organism and also has a remarkable curative effect on tumor inhibition by being matched with konjac powder, maltodextrin, citric acid, xylitol and sucralose.
(2) The powder drink product obtained by the invention has delicious and pure taste, natural nutrition, suitability for long-term use, simple preparation method, uniform mixing by adopting an equivalent increasing method in the preparation process, and the specific operation method is as follows: taking a small amount of components and an equal amount of components with large amount, simultaneously placing the components in a mixing machine for uniformly mixing, adding the components with large amount and equal amount to the mixture for uniformly mixing, and increasing the times till the components with large amount are completely added. The invention adopts an equivalent incremental method for mixing, so that the components with very different proportions in the formula can be mixed more uniformly, and the mixing time is saved.
Drawings
FIG. 1 is a schematic process flow diagram of the present invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. Unless otherwise specified, 1 part in the present invention means 1 g.
Example 1
A sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following raw materials in parts by weight:
2000 parts of seabuckthorn fruit powder, 1200 parts of maltodextrin, 300 parts of pumpkin powder, 200 parts of soybean peptide powder, 200 parts of pineapple powder, 100 parts of yeast β -glucan, 100 parts of cranberry fruit powder, 100 parts of konjac powder, 60 parts of lutein ester microcapsule powder, 50 parts of Plantago ovata husk powder, 20 parts of fructus momordicae powder, 5 parts of vitamin C, 10 parts of xylitol, 10 parts of citric acid and 2 parts of sucralose.
As shown in fig. 1, a preparation method of a sea buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following steps:
(1) grinding and sieving the raw materials respectively to obtain fructus Hippophae powder with particle size of 178 μm, maltodextrin, fructus Cucurbitae Moschatae powder, semen glycines peptide powder, fructus Ananadis Comosi powder, yeast β -dextran, cranberry fruit powder, rhizoma Amorphophalli powder, lutein ester microcapsule powder, Plantago ovata seed husk powder, fructus Siraitiae Grosvenorii powder, vitamin C, xylitol, citric acid, and sucralose for use;
(2) weighing the momordica grosvenori powder, the vitamin C, the xylitol and the citric acid according to the weight parts, and mixing for 28 minutes to obtain a first mixed material; mixing and stirring the first mixed material and sucralose powder in an equivalent increasing mode for 28 minutes to obtain a second mixed material;
(3) and mixing the second mixed material with the seabuckthorn fruit powder, maltodextrin, pumpkin powder, soybean peptide powder, pineapple powder, yeast β -glucan, cranberry fruit powder, konjak powder, lutein ester microcapsule powder and Plantago ovata seed husk powder in parts by weight for 110 minutes to obtain the seabuckthorn solid beverage.
Example 2
A sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following raw materials in parts by weight:
2200 parts of seabuckthorn fruit powder, 1600 parts of maltodextrin, 400 parts of pumpkin powder, 280 parts of soybean peptide powder, 280 parts of pineapple powder, 160 parts of yeast β -glucan, 220 parts of cranberry fruit powder, 150 parts of konjac powder, 80 parts of lutein ester microcapsule powder, 80 parts of psyllium husk powder, 40 parts of momordica grosvenori powder, 8 parts of vitamin C, 15 parts of xylitol, 20 parts of citric acid and 3 parts of sucralose.
A preparation method of sea buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following steps:
(1) grinding and sieving the raw materials respectively to obtain fructus Hippophae powder, maltodextrin, fructus Cucurbitae Moschatae powder, semen glycines peptide powder, fructus Ananadis Comosi powder, yeast β -dextran, cranberry fruit powder, rhizoma Amorphophalli powder, lutein ester microcapsule powder, Plantago ovata seed husk powder, fructus Siraitiae Grosvenorii powder, vitamin C, xylitol, citric acid, and sucralose with particle size of 150 μm for use;
(2) weighing the momordica grosvenori powder, the vitamin C, the xylitol and the citric acid according to the weight parts, and mixing for 29 minutes to obtain a first mixed material; mixing and stirring the first mixed material and sucralose powder for 29 minutes in an equivalent increasing mode to obtain a second mixed material;
(3) and mixing the second mixed material with the seabuckthorn fruit powder, maltodextrin, pumpkin powder, soybean peptide powder, pineapple powder, yeast β -glucan, cranberry fruit powder, konjak powder, lutein ester microcapsule powder and Plantago ovata seed husk powder in parts by weight for 115 minutes to obtain the seabuckthorn solid beverage.
Example 3
A sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following raw materials in parts by weight:
2300 parts of seabuckthorn fruit powder, 1700 parts of maltodextrin, 600 parts of pumpkin powder, 330 parts of soybean peptide powder, 330 parts of pineapple powder, 180 parts of yeast β -glucan, 240 parts of cranberry fruit powder, 180 parts of konjac powder, 100 parts of lutein ester microcapsule powder, 100 parts of psyllium husk powder, 25 parts of momordica grosvenori powder, 12 parts of vitamin C, 20 parts of xylitol, 25 parts of citric acid and 4 parts of sucralose.
A preparation method of sea buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following steps:
(1) grinding, grinding and sieving the raw materials respectively to obtain fructus Hippophae powder with particle size of 104 μm, maltodextrin, fructus Cucurbitae Moschatae powder, semen glycines peptide powder, fructus Ananadis Comosi powder, yeast β -dextran, cranberry fruit powder, rhizoma Amorphophalli powder, lutein ester microcapsule powder, Plantago ovata seed husk powder, fructus Siraitiae Grosvenorii powder, vitamin C, xylitol, citric acid, and sucralose for use;
(2) weighing the momordica grosvenori powder, the vitamin C, the xylitol and the citric acid according to the weight parts, and mixing for 30 minutes to obtain a first mixed material; mixing and stirring the first mixed material and sucralose powder in an equivalent increasing mode for 30 minutes to obtain a second mixed material;
(3) and mixing the second mixed material with the seabuckthorn fruit powder, maltodextrin, pumpkin powder, soybean peptide powder, pineapple powder, yeast β -glucan, cranberry fruit powder, konjak powder, lutein ester microcapsule powder and Plantago ovata seed husk powder in parts by weight for 120 minutes to obtain the seabuckthorn solid beverage.
Example 4
A sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following raw materials in parts by weight:
2400 parts of sea buckthorn fruit powder, 1400 parts of maltodextrin, 550 parts of pumpkin powder, 350 parts of soybean peptide powder, 350 parts of pineapple powder, 220 parts of yeast β -glucan, 280 parts of cranberry fruit powder, 200 parts of konjac powder, 110 parts of lutein ester microcapsule powder, 100 parts of psyllium husk powder, 30 parts of momordica grosvenori powder, 15 parts of vitamin C, 25 parts of xylitol, 18 parts of citric acid and 4.5 parts of sucralose.
A preparation method of sea buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following steps:
(1) grinding, grinding and sieving the raw materials respectively to obtain fructus Hippophae powder with particle size of 74 μm, maltodextrin, fructus Cucurbitae Moschatae powder, semen glycines peptide powder, fructus Ananadis Comosi powder, yeast β -dextran, cranberry fruit powder, rhizoma Amorphophalli powder, lutein ester microcapsule powder, Plantago ovata seed husk powder, fructus Siraitiae Grosvenorii powder, vitamin C, xylitol, citric acid, and sucralose for use;
(2) weighing the momordica grosvenori powder, the vitamin C, the xylitol and the citric acid according to the weight parts, and mixing for 31 minutes to obtain a first mixed material; mixing and stirring the first mixed material and sucralose powder in an equivalent increasing mode for 31 minutes to obtain a second mixed material;
(3) and mixing the second mixed material with the seabuckthorn fruit powder, maltodextrin, pumpkin powder, soybean peptide powder, pineapple powder, yeast β -glucan, cranberry fruit powder, konjak powder, lutein ester microcapsule powder and Plantago ovata seed husk powder in parts by weight for 125 minutes to obtain the seabuckthorn solid beverage.
Example 5
A sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following raw materials in parts by weight:
2500 parts of sea buckthorn fruit powder, 1500 parts of maltodextrin, 500 parts of pumpkin powder, 300 parts of soybean peptide powder, 300 parts of pineapple powder, 200 parts of yeast β -glucan, 200 parts of cranberry fruit powder, 200 parts of konjac powder, 120 parts of lutein ester microcapsule powder, 100 parts of psyllium husk powder, 30 parts of momordica grosvenori powder, 10 parts of vitamin C, 30 parts of xylitol, 15 parts of citric acid and 5 parts of sucralose.
A preparation method of sea buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following steps:
(1) grinding, grinding and sieving the raw materials respectively to obtain fructus Hippophae powder with particle size of 74 μm, maltodextrin, fructus Cucurbitae Moschatae powder, semen glycines peptide powder, fructus Ananadis Comosi powder, yeast β -dextran, cranberry fruit powder, rhizoma Amorphophalli powder, lutein ester microcapsule powder, Plantago ovata seed husk powder, fructus Siraitiae Grosvenorii powder, vitamin C, xylitol, citric acid, and sucralose for use;
(2) weighing the momordica grosvenori powder, the vitamin C, the xylitol and the citric acid according to the weight parts, and mixing for 32 minutes to obtain a first mixed material; mixing and stirring the first mixed material and sucralose powder in an equivalent increasing mode for 32 minutes to obtain a second mixed material;
(3) and mixing the second mixed material with the seabuckthorn fruit powder, maltodextrin, pumpkin powder, soybean peptide powder, pineapple powder, yeast β -glucan, cranberry fruit powder, konjak powder, lutein ester microcapsule powder and Plantago ovata seed husk powder in parts by weight for 120 minutes to obtain the seabuckthorn solid beverage.
Example 6
A sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following raw materials in parts by weight:
2500 parts of sea buckthorn fruit powder, 1500 parts of maltodextrin, 500 parts of pumpkin powder, 300 parts of soybean peptide powder, 300 parts of pineapple powder, 200 parts of yeast β -glucan, 200 parts of cranberry fruit powder, 200 parts of konjac powder, 120 parts of lutein ester microcapsule powder, 100 parts of psyllium husk powder, 30 parts of momordica grosvenori powder, 10 parts of vitamin C, 30 parts of xylitol, 15 parts of citric acid and 5 parts of sucralose.
A preparation method of sea buckthorn solid beverage for enhancing immunity and inhibiting tumors comprises the following steps:
(1) grinding, grinding and sieving the raw materials respectively to obtain cranberry sea buckthorn fruit powder with the particle size of 178 microns, maltodextrin, pumpkin powder, soybean peptide powder, pineapple powder, yeast β -glucan, cranberry fruit powder, konjac powder, lutein ester microcapsule powder, psyllium husk powder, momordica grosvenori powder, vitamin C, xylitol, citric acid and sucralose for later use;
(2) weighing the momordica grosvenori powder, the vitamin C, the xylitol and the citric acid according to the weight parts, and mixing for 30 minutes to obtain a first mixed material; mixing and stirring the first mixed material and sucralose powder in an equivalent increasing mode for 30 minutes to obtain a second mixed material;
(3) and mixing the second mixed material with the seabuckthorn fruit powder, maltodextrin, pumpkin powder, soybean peptide powder, pineapple powder, yeast β -glucan, cranberry fruit powder, konjak powder, lutein ester microcapsule powder and Plantago ovata seed husk powder in parts by weight for 120 minutes to obtain the seabuckthorn solid beverage.
Animal experiments
1. Materials and methods
1.1, animal:
selecting female Kunming mice, wherein the physical mass of each mouse is (18-22) g, and the mice are purchased from Wuhan biological products institute, and the qualification number is as follows: SCXK 2017-.
1.2, medicine:
weighing 6g of the sea-buckthorn solid beverage obtained in the embodiment 6 of the invention, dissolving and diluting the sea-buckthorn solid beverage with 200m of purified water L, filtering the sea-buckthorn solid beverage by using a 80-mesh screen, and concentrating the obtained filtrate to 0.03g/ml for later use, which is hereinafter referred to as a product;
1.3 dose grouping
Setting low, medium and high 3 dose groups (1, 2 and 3g/kg BW) according to the 10, 20 and 30 times of the recommended daily intake of human body, wherein the BW is enlarged, dividing the groups into 4 experimental groups, each experimental group comprises a negative control group, a solvent control group, a low, medium and high dose group, and the number of experimental animals in each dose group is 10. Immunization experiment a group of experiments of phagocytosis of chicken red blood cells by mouse abdominal cavity macrophages; immune experiment two groups carry out the determination of the ratio of lymph organs to body weight and the carbon clearance experiment; three groups of immunization experiments were performed for measurement of serum hemolysin and detection of antibody producing cells. The test is started after the mice are continuously gazed for 30 days, each experimental group is given by oral gavage according to the volume of 10ml/10gBW, the negative control group is given distilled water, and the solvent control group is given soybean oil.
1.4 test methods
1.4.1 experiment of phagocytosis of chicken red blood cells by abdominal macrophages
The method comprises the following steps of preparing 20% chicken erythrocyte suspension by an in-vivo method, injecting 1m L suspension into the abdominal cavity of each mouse, killing the animal after 30 minutes, fixing the mouse erythrocyte suspension on a mouse plate in an inverted position, opening the abdomen, injecting 2m L physiological saline into the abdominal cavity, rotating the mouse plate for 1 minute, sucking 1m L abdominal washing liquid, evenly dripping the abdominal washing liquid on 2 glass slides, incubating for 30 minutes at 37 ℃, rinsing with the physiological saline after finishing the incubation, drying in the air, fixing the mouse erythrocyte suspension by using 1: 1 acetone-methanol solution, staining for 3 minutes by using 4% Giemsa-phosphoric acid buffer solution, rinsing with distilled water, drying in the air, counting 100 macrophages under a microscope, and calculating the phagocytosis rate and the phagocytosis index according to the following formula:
phagocytosis ratio (%) — number of macrophages that phagocytose chicken red blood cells/number of macrophages counted × 100
Phagocytosis index-total number of phagocytosed chicken red blood cells/number of macrophages counted
1.4.2 mouse carbon clearance test
The method comprises injecting diluted India ink (10m L/kg) into mouse tail vein according to body weight, and immediately timing the injection of the ink, respectively, collecting blood 20 μ L from angular venous plexus at 2 and 10 min after the injection of the ink, and immediately adding the obtained blood to 2m L0.1.1% Na2CO3In solution, 0.1m L of each was pipetted into a 96-well microplate, and the Optical Density (OD) was measured at 600nm wavelength with a microplate reader and Na2CO3The solution was used as a negative control. The mice were sacrificed. The liver and spleen were taken, and blood stain on the surface of the viscera was blotted with filter paper and weighed. The ability of the mice to clear carbon is expressed as a phagocytic index. The phagocytic index a was calculated as follows. The phagocytosis index of the test sample group is obviously higher than that of the control group, and the positive result of the experiment can be judged.
K＝(LgOD1-lgOD2)/(t2-t1)
Phagocytosis index a ═ body weight/(liver weight + spleen weight) × K1/3
1.4.3 measurement of serum hemolysin
The method comprises the steps of hemagglutination, sheep blood taking, washing for 3 times by using normal saline, centrifuging for 10 minutes (2000 r/min) each time, preparing 2% (v/v) cell suspension by using packed SRBC by using the normal saline, injecting 0.2m L into the abdominal cavity of each mouse for immunization, removing eyeballs, taking blood from the eyeballs, placing the blood in a centrifugal tube for about 1 hour, stripping coagulated blood from the tube wall, fully separating out serum, centrifuging for 10 minutes at 2000 r/min, collecting the serum, carrying out agglutination reaction, diluting the serum by using the normal saline in a multiple ratio, respectively placing the serum with different dilution degrees in a trace hemagglutination experimental plate with 100 mu L per hole, adding 100 mu L0.5% (v/v) SRBC suspension into the sample, mixing the mixture, placing the sample in a wet flat disc, covering the flat disc, incubating the sample in a 37 ℃ incubator for 3 hours, observing the hemagglutination degree, and calculating the number of antibodies according to the level of the hemaggl.
1.4.4 detection of antibody-producing cells
Washing defibered sheep blood with physiological saline for 3 times, centrifuging (2000 r/min) for 10 minutes each time, injecting 2% (v/v) SRBC 0.2m L into the abdominal cavity of each mouse, dislocating and killing the cervical vertebra of the mouse immunized by the SRBC for 4-5 days, taking out the spleen, placing the spleen in a small plate containing hank's solution, slightly grinding the spleen to prepare cell suspension, filtering the cell suspension by a 200-mesh screen, centrifuging (1000/min) for 10 minutes, washing the cell suspension for 2 times by the hank's solution, finally suspending the cell in 5m L RPMI1640 culture solution, counting the cells, and adjusting the cell concentration to 5 × 106/m L.
The plaque is determined by heating and dissolving surface layer culture medium (1g agarose added with double distilled water to 100m L), placing in 45-50 deg.C water bath, keeping temperature, mixing with equal amount of hank's solution with pH of 7.2-7.4 and 2 times concentration, subpackaging into small tubes with 0.5m L per tube, adding 50 μ L10% SRBC (v/v, prepared with SA buffer solution), and 20 μ L spleen cell suspension (5 × 10)6Pieces/m L), quickly mixing, pouring onto a slide with a brushed agarose thin layer, making parallel pieces, after the agar is solidified, horizontally buckling the slide on a slide rack, putting the slide rack into a carbon dioxide incubator to incubate for 1.5h, adding a complement (1: 8) diluted by SA buffer solution into a groove of the slide rack, continuously incubating for 1.5h, and counting the number of hemolytic plaques.
2. Results
2.1 Effect of this product on mouse body weight
The weight of the mice in each experimental group in the table 1, the table 2 and the table 3 before and after the test is compared with the weight of the solvent control group and the weight of the negative control group, and the difference is not significant, which indicates that the sea buckthorn solid beverage has no obvious influence on the weight of the mice.
TABLE 1 animal body weight records before and after experiment set (x + -s)
Figure BSA0000176954620000131
TABLE 2 animal body weight records before and after two experimental groups (x + -s)
Figure BSA0000176954620000141
TABLE 3 animal body weight records before and after three experimental groups (x + -s)
Figure BSA0000176954620000142
2.2 Effect of solid Hippophae rhamnoides drink on animal lymph organ/body weight ratio
Table 4 the thymus/body weight ratio and spleen/body weight ratio of each group of animals were compared with the solvent control group and the negative control group, and the differences were not significant (p > 0.05).
TABLE 4 Effect on animal lymphatic organ/body weight ratio (x. + -. s)
Figure BSA0000176954620000143
Note: the (solvent) indicates the comparison to the solvent control group, and the (negative) indicates the comparison to the negative control group.
2.3 influence of solid beverage of Hippophae rhamnoides on carbon clearing function
As can be seen from Table 5, the high-dose group of fructus Hippophae solid beverage significantly increased the phagocytic index of mouse carbon clearance (P < 0.05) compared with the solvent control group and the negative control group.
TABLE 5 Effect on mouse carbon clearance function (x. + -.s)
Figure BSA0000176954620000151
Note: the (solvent) indicates the comparison to the solvent control group, and the (negative) indicates the comparison to the negative control group.
2.4 influence of solid beverage of seabuckthorn on phagocytosis of chicken red blood cells by macrophages in abdominal cavity
As can be seen from Table 6, the high dose group of the solid beverage of seabuckthorn significantly improved the phagocytosis rate and phagocytosis index (P < 0.05) compared with the solvent control group and the negative control group.
TABLE 6 Effect on phagocytic function of mouse peritoneal macrophages (x. + -. s)
Figure BSA0000176954620000152
Note: the (solvent) indicates the comparison to the solvent control group, and the (negative) indicates the comparison to the negative control group.
2.5 Effect of sea-buckthorn solid beverage on mouse serum hemolysin titer level
As can be seen from Table 7, the content of serum hemolysin (P < 0.05) in the high dose group of seabuckthorn solid beverage can be significantly increased compared with the solvent control group and the negative control group.
TABLE 7 Effect on mouse hemolysin titer levels (x. + -.s)
Figure BSA0000176954620000153
Note: the (solvent) indicates the comparison to the solvent control group, and the (negative) indicates the comparison to the negative control group.
2.6 Effect of solid beverage of Hippophae rhamnoides on antibody-producing cell function
As can be seen from Table 8, the high dose group of solid beverage of Hippophae rhamnoides significantly enhanced the antibody-producing cell capacity (P < 0.01, P < 0.05) compared to the solvent control group and the negative control group.
TABLE 8 Effect on antibody-producing cell function (x. + -.s)
Figure BSA0000176954620000161
Note: the (solvent) indicates the comparison to the solvent control group, and the (negative) indicates the comparison to the negative control group.
3. Discussion of the related Art
(1) The sea-buckthorn solid beverage can obviously increase the content of the mouse serum hemolysin;
(2) the sea-buckthorn solid beverage can obviously enhance the function of macrophages in abdominal cavity of a mouse for phagocytosing chicken red blood cells;
(3) the sea-buckthorn solid beverage can obviously enhance the carbon clearance capability of mice;
(4) the sea-buckthorn solid beverage can obviously enhance the antibody cell-forming capacity;
(5) the sea-buckthorn solid beverage has no negative effect on improving the immunity of mice.
And (3) clinical trials:
1) general data: the inventor carries out clinical observation on 90 cases, wherein 47 cases are male, and 43 cases are female; age 18 years minimum, 60 years maximum; 37 cases of skin diseases, 30 cases of hepatitis, 23 cases of qi-invigorating in intestines and stomach, and other 4 cases (including qi-kick, physical weakness, aversion to cold, chronic pharyngitis, tonsillitis, common cold and the like).
2) The treatment method comprises the following steps: the product is taken once a day by 6g each time.
3) The evaluation standard of curative effect is as follows:
and (3) curing: clinical symptoms disappear completely, pain and discomfort disappear completely, and normal life is restored.
The method has the following advantages: all or major symptoms are eliminated significantly, pain and discomfort are eliminated significantly, but mild symptoms appear, and normal life is only partially restored.
And (4) invalidation: no obvious change in symptoms and signs.
4) The treatment results are as follows: as shown in table 4
TABLE 4
General case Cure of disease Is effective Invalidation High efficiency
90 62 24 4 96％
5) Typical clinical cases are:
1. zhangzhi, male, 28 years old, and 2017 year old herpes zoster, and the symptoms disappear after taking the product for 5 days.
2. When you make you, women, age 49, 2016 have diarrhea, and after taking this product for 3 days, the symptoms disappear.
3. Yuanzhi, male, 56 years old, and 2017 years old have sore throat and cough, and the patient can be cured after taking the product for 3 days.
4. The tonsillitis of women aged 52 years old in 2017 is cured after taking the product for 3 days.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims (10)

1. A sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors is characterized by comprising the following raw materials in parts by weight:
3000 parts of sea buckthorn fruit powder, 1800 parts of maltodextrin 1200, 700 parts of pumpkin powder, 400 parts of soybean peptide powder, 400 parts of pineapple powder, 300 parts of yeast β -glucan 100, 300 parts of cranberry fruit powder, 300 parts of konjac powder, 60-180 parts of lutein ester microcapsule powder, 50-150 parts of Plantago ovata husk powder, 20-40 parts of Hance fruit powder, 5-15 parts of vitamin C, 10-50 parts of xylitol, 10-50 parts of citric acid and 2-8 parts of sucralose.
2. The sea-buckthorn solid beverage according to claim 1, comprising the following raw materials in parts by weight:
2200-2800 parts of sea buckthorn fruit powder, 1300-1700 parts of maltodextrin, 320-600 parts of pumpkin powder, 220-380 parts of soybean peptide powder, 220-380 parts of pineapple powder, β -280 parts of glucan, 120-280 parts of cranberry powder, 120-280 parts of konjac powder, 80-160 parts of lutein ester microcapsule powder, 80-120 parts of Plantago ovata husk powder, 22-38 parts of Momordica grosvenori powder, 6.8-14.8 parts of vitamin C, 12-40 parts of xylitol, 12-40 parts of citric acid and 2.2-7.8 parts of sucralose.
3. The sea-buckthorn solid beverage according to claim 1, comprising the following raw materials in parts by weight:
2500 parts of sea buckthorn fruit powder, 1500 parts of maltodextrin, 500 parts of pumpkin powder, 300 parts of soybean peptide powder, 300 parts of pineapple powder, 200 parts of yeast β -glucan, 200 parts of cranberry fruit powder, 200 parts of konjac powder, 120 parts of lutein ester microcapsule powder, 100 parts of psyllium husk powder, 30 parts of momordica grosvenori powder, 10 parts of vitamin C, 30 parts of xylitol, 15 parts of citric acid and 5 parts of sucralose.
4. The sea buckthorn solid beverage according to claim 1, further comprising 10 parts by weight of vitamin C.
5. A method for preparing the solid beverage of seabuckthorn fruit with immunity enhancing and tumor suppressing effects as claimed in any one of claims 1 to 3, comprising the steps of:
(1) grinding and sieving the raw materials respectively to obtain fructus Hippophae powder, maltodextrin, fructus Cucurbitae Moschatae powder, soybean peptide powder, fructus Ananadis Comosi powder, yeast β -dextran, cranberry fruit powder, rhizoma Amorphophalli powder, lutein ester microcapsule powder, Plantago ovata seed husk powder, fructus Siraitiae Grosvenorii powder, vitamin C, xylitol, citric acid and sucralose powder for use;
(2) weighing the momordica grosvenori powder, the vitamin C, the xylitol and the citric acid in parts by weight, and mixing to obtain a first mixed material; mixing and stirring the first mixed material and the sucralose powder in an equivalent increasing mode to obtain a second mixed material;
(3) and uniformly mixing the second mixed material with the seabuckthorn fruit powder, maltodextrin, pumpkin powder, soybean peptide powder, pineapple powder, yeast β -glucan, cranberry fruit powder, konjak powder, lutein ester microcapsule powder and Plantago ovata seed husk powder to obtain the seabuckthorn solid beverage.
6. The sea buckthorn solid beverage according to claim 5, wherein the particle size of the sea buckthorn fruit powder, maltodextrin, pumpkin powder, soybean peptide powder, pineapple powder, yeast β -glucan, cranberry fruit powder, konjac powder, lutein ester microcapsule powder, psyllium husk powder, momordica grosvenori powder, vitamin C, xylitol, citric acid and sucralose powder in step (1) is not more than 178 microns.
7. The sea-buckthorn solid beverage according to claim 5, wherein the mixing time in the step (2) is 28-32 minutes, and the mixing and stirring time is 28-32 minutes.
8. The sea-buckthorn solid beverage according to claim 7, wherein the mixing time in the step (2) is 30 minutes, and the mixing and stirring time is 30 minutes.
9. The seabuckthorn solid beverage as claimed in claim 5, wherein the time for mixing in step (3) is 110-130 minutes.
10. The hippophae rhamnoides solid beverage according to claim 9, wherein the time for mixing uniformly in step (3) is 2 hours.
CN201910004101.9A 2019-01-03 2019-01-03 Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof Pending CN111387394A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910004101.9A CN111387394A (en) 2019-01-03 2019-01-03 Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910004101.9A CN111387394A (en) 2019-01-03 2019-01-03 Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof

Publications (1)

Publication Number Publication Date
CN111387394A true CN111387394A (en) 2020-07-10

Family

ID=71410703

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910004101.9A Pending CN111387394A (en) 2019-01-03 2019-01-03 Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof

Country Status (1)

Country Link
CN (1) CN111387394A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112042846A (en) * 2020-09-16 2020-12-08 深圳鼎盛和兴科技有限公司 Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof
CN112401099A (en) * 2020-11-21 2021-02-26 深圳福源堂生物科技有限公司 Preparation method of sea-buckthorn prebiotics solid beverage

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112042846A (en) * 2020-09-16 2020-12-08 深圳鼎盛和兴科技有限公司 Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof
CN112401099A (en) * 2020-11-21 2021-02-26 深圳福源堂生物科技有限公司 Preparation method of sea-buckthorn prebiotics solid beverage

Similar Documents

Publication Publication Date Title
CN101695376B (en) Health-care food for preventing diabetes
CN103054113B (en) Composite fruit-vegetable juice beverage with blood sugar decreasing effect
CN106942542A (en) A kind of cubilose beverage of the grain of suspension containing nostoc and preparation method thereof
CN101023802A (en) Series products of all-nutrient green health-care bean vermicellic and instant bean vermicellic and preparing method
CN103463457B (en) Composite chewable tablet of Herba Dendrobii Hericium erinaceus (Bull. Ex Fr.) Pers. and preparation method thereof
CN103416534B (en) Selenium-rich stomach-nourishing tea and production method thereof
CN105410346A (en) Cultured penaeus vannamei survival rate improving traditional Chinese medicine preparation and preparation method thereof
CN107156828A (en) A kind of full nutrition formula food suitable for patients with inflammatory bowel
CN108014150B (en) Application of natural medicine composition in preparing anti-hypoxia and anti-radiation medicine or food
CN110585302A (en) Traditional Chinese medicine combined granular tea for treating chronic pharyngitis and preparation method thereof
Yampolsky et al. Apricot (lat. Prunus armeniaca Lin.)
CN110192583A (en) With the composition and application for promoting intestinal health, control weight and raising to be immunized
KR100770775B1 (en) Functional drink for immune- enhancing and Manufacturing method thereof
CN108813501B (en) Health-preserving honey paste with functions of clearing heat, moistening lung, relieving cough, reducing phlegm, relieving asthma and regulating human body functions
CN111387394A (en) Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof
KR101122586B1 (en) Hang over beverage containing freeze-dring Abalone flesh and It&#39;s manufacturing method
CN107660671A (en) A kind of water chestnut skin drinks
CN106722258A (en) A kind of astaxanthin colla corii asini cake and preparation method thereof
CN109527577A (en) A kind of composition with sobering up and liver protecting functions
CN112042846A (en) Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof
CN114831304A (en) Hericium erinaceus-gordon euryale bird&#39;s nest porridge and preparation method thereof
CN113841884A (en) Formula of instant meal replacement powder for improving nutrient metabolism and preparation method thereof
CN107183597A (en) A kind of indigo fruit functional food
CN108813500B (en) Health-preserving honey paste with effects of tonifying middle-jiao and Qi, nourishing blood and soothing nerves and regulating human body functions
KR20000037815A (en) Healthy food contained natural medicine materials

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20200710