CN101595991B - Preparation method of canna edulis ker polysaccharide polyphenol complex - Google Patents
Preparation method of canna edulis ker polysaccharide polyphenol complex Download PDFInfo
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- CN101595991B CN101595991B CN2009100543187A CN200910054318A CN101595991B CN 101595991 B CN101595991 B CN 101595991B CN 2009100543187 A CN2009100543187 A CN 2009100543187A CN 200910054318 A CN200910054318 A CN 200910054318A CN 101595991 B CN101595991 B CN 101595991B
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Abstract
The invention relates to a preparation method of canna edulis ker polysaccharide polyphenol complex, comprising the following steps: the waste residue of canna edulis ker is prepared, water is added and mixed with the waste residue of canna edulis ker to obtain solution; the pH value of the solution is adjusted to 7.5-8.5; thermo-stabilization alpha-amylase is added to ensure that the mass fraction of the amylase is 0.05-0.1%, and enzymolysis is carried out at the temperature of 95-100 DEG C for 30-60 minutes; when solution obtained in the second step is cooled to the temperature of 50-60 DEG C, alkalescent papain is added, the mass fraction of the alkalescent papain is 0.1-0.2%, and enzymolysis is carried out for 30-60 minutes; then, lipase is added, and the mass fraction of the lipase reaches 0.1-0.2%, and enzymolysis is carried out for 30-60 minutes; the pH value of the solution is adjusted to 9-11, and hydrogen peroxide with the volume ratio being 4-7% is added to stir for 30-60 minutes at the temperature of 20-30 DEG C; filter residue is obtained after filtration, is washed to be neutral, is dried and is pulverized to obtain the canna edulis ker polysaccharide polyphenol complex. The invention has large raw material output, low price, simple technology and lower cost, recycles waste residues and reduces environment pollution.
Description
Technical field
The present invention relates to a kind of preparation method of food technology field, specifically is a kind of preparation method of canna edulis ker polysaccharide polyphenol complex.
Background technology
Functional bajiao banana taro dietary fiber is a kind of polysaccharide polyphenol complex.Polysaccharide in the bajiao banana taro dietary fiber is to be the natural macromolecular material of main body with the cellulose in the bajiao banana taro.Polysaccharide is the important component part of all living organisms, and required different physiological roles is relevant with earning a bare living.Polysaccharide be proved to be have the enhancing body immunologic function, antiviral, prevent and treat important physical functions such as diabetes, antitumor, anti-inflammatory, anticoagulation, radioresistance, reducing blood lipid.Polyphenol is the general name of polyhydric phenols in the bajiao banana taro.Plant polyphenol be proved to be have anti-oxidant, hemostasis and anti-inflammation, the disease-resistant bacterium of detoxifcation, anti-carious tooth, antiallergy, anti-sudden change, antitumor, anticancer, anti-nicotine, treatment cataract and angiocardiopathy, promotion immunity, delay senility, important physical functions such as sun-proof, skin whitening, moisturizing, crease-resistant and maintenance skin elasticity, be mainly used in health products such as antineoplastic, cardiovascular health medicine, anti-virus formulation, anti-diabetic and obesity, non-oxidizability food additives, antistaling agent, color preserving agent, deodorant etc.At present, along with the rise of the heat that goes back to nature, the plant polyose polyphenol is used by extensive studies.
Bajiao banana taro (Canna edulis Ker) main product is in provinces and regions, the west and south such as China Guizhou, Yunnan and Guangxi, its adaptability is strong, impoverishment tolerant, be easy to plantation, output is very big.At present, the bajiao banana taro mainly is used to produce starch and bean vermicelli.Canna edulis ker waste residue extracts remaining residue behind the starch for the bajiao banana taro, and these waste residue water content are high, are easy to fermentation; Except that being processed on a small quantity the feed; The overwhelming majority is taken as refuse and throws away, and this has not only caused the huge waste of resource, and severe contamination local environment.Therefore recycle the added value that waste residue must improve canna edulis ker waste residue greatly, have good economic benefit, and alleviated environmental pollution, have the important social meaning.
Summary of the invention
The objective of the invention is to overcome the deficiency of prior art, a kind of preparation method of canna edulis ker polysaccharide polyphenol complex is provided.Raw material output of the present invention is big, and is cheap, and technology is simple, and cost is lower, and waste residue is recycled, and reduced environmental pollution.
The present invention realizes through following technical scheme, the present invention relates to a kind of preparation method of canna edulis ker polysaccharide polyphenol complex, comprises the steps:
Step 1, the preparation canna edulis ker waste residue adds the water mixing and obtains solution;
Step 2, the pH value of regulator solution is 7.5～8.5, adds thermally-stabilised AMS, makes diastatic mass fraction reach 0.5～1 ‰, 95～100 ℃ of following enzymolysis 30～60 minutes;
Step 3, cooling solution to 50～60 ℃ add alkaline papain, make the mass fraction of protease reach 1～2 ‰, enzymolysis 30～60 minutes; Add lipase afterwards, make its mass fraction reach 1～2 ‰, enzymolysis 30～60 minutes;
Step 4 is adjusted to 9～11 with the pH value of solution, adds volume fraction and be 4～7% hydrogen peroxide solution, and 20～30 ℃ were stirred 30～60 minutes;
Step 5, filtering solution gets filter residue, and washing is to neutral, and drying under reduced pressure or vacuum freeze drying are pulverized, and obtain canna edulis ker polysaccharide polyphenol complex.
In the step 1, said preparation is specially: canna edulis ker waste residue in 50～60 ℃ of dryings, was pulverized 40 mesh sieves, cross 160 mesh sieves afterwards, 25～40 ℃ of following water washing and filterings get filter residue.
In the step 1, saidly be mixed into sonicated 10～30 minutes.
In the step 1, said solution, the mass ratio of water and filter residue is (5～10) in the solution: 1.
In the step 5, said drying under reduced pressure is to carry out under 40～50 ℃.
The present invention has following beneficial effect: the present invention has carried out effective removal to starch, protein and fat, has improved extraction efficiency, has reduced the probability that chemical method destroys polyphenol; The present invention adopts hydrogen peroxide solution that dietary fiber is decoloured at normal temperatures, adopts low-temperature vacuum drying or freeze drying to reduce the destruction to polyphenol.Raw material output of the present invention is big, and is cheap, and technology is simple, and cost is lower, and waste residue is recycled, and reduced environmental pollution.The canna edulis ker polysaccharide polyphenol complex that the present invention obtains can be widely used in can be used as a kind of functional food additives simultaneously and being used for food in the health products such as fat-reducing, defaecation, toxin expelling, improves nutritive value, can also be used for cosmetics.
Description of drawings
Fig. 1 is preparation technology's flow chart of the present invention;
Fig. 2 is the solid of polysaccharide polyphenol complex
13The C-NMR wave spectrogram.
The specific embodiment
Following instance will combine accompanying drawing that the present invention is described further.Present embodiment provided detailed embodiment and process, but protection scope of the present invention is not limited to following embodiment being to implement under the prerequisite with technical scheme of the present invention.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.
As shown in Figure 1, preparation technology's flow process of the embodiment in the specific embodiment is implemented according to the technology that shows in the accompanying drawing, and is specific as follows:
Embodiment 1
Step 1, canna edulis ker waste residue come from Guizhou Zi Yunjia and Chemical Industry Science Co., Ltd, with canna edulis ker waste residue in 50 ℃ of dryings; Pulverize, cross 40 mesh sieves, cross 160 mesh sieves afterwards to remove partial starch; Residue in the collection screen; The water that adds 8 times of volumes, in 30 ℃ with filter residue agitator treating 20 minutes, cross the leaching filter residue with double gauze; Add the water of 8 times of filter residue volumes in the filter residue, sonicated 20 minutes obtains solution;
Step 2, the pH value of regulator solution is 8.2, adds thermally-stabilised AMS, makes diastatic mass fraction reach 0.6 ‰, 95 ℃ of following 800rpm stirred enzymolysis 60 minutes;
Step 3, the solution to 60 that cooling step two finally obtains ℃ adds alkaline papain, makes the mass fraction of protease reach 1 ‰, and 800rpm stirred enzymolysis 60 minutes; Add lipase afterwards, make its mass fraction reach 1 ‰, 800rpm stirs enzymolysis 60 minutes;
Step 4, the pH value of the solution that step 3 is finally obtained is adjusted to 11, adds volume fraction and be 4% hydrogen peroxide solution, and 25 ℃ were stirred 60 minutes;
Step 5, the solution that filtration step four finally obtains gets filter residue, and washing is to neutral; 40 ℃ of following drying under reduced pressure 24 hours are pulverized, and the filter residue that drying is good is pulverized 80 orders with common pulverizer; Pulverize 300 orders with micronizer then, obtain canna edulis ker polysaccharide polyphenol complex.
The utilization solid
13C-NMR identifies that to the polysaccharide polyphenol complex that obtains the result sees Fig. 2.Wherein, chemical shift is that the signal of 172～174ppm is a carbonyl absorption peak, and chemical shift is that the multiplet of 127～155ppm is a double bond absorption peak, and this has shown the existence of polyphenol compound.Chemical shift is that 105ppm place sharp-pointed unimodal is the absworption peak of polysaccharide end group carbon, and chemical shift is that the multiplet of 54～72ppm is the absworption peak of all the other carbon of polysaccharide, and these peak-to-peak signals are stronger, shown the existence of a large amount of polysaccharide.
Embodiment 2
Step 1, canna edulis ker waste residue come from Guizhou Zi Yunjia and Chemical Industry Science Co., Ltd, with canna edulis ker waste residue in 60 ℃ of dryings; Pulverize, cross 40 mesh sieves, cross 160 mesh sieves afterwards to remove partial starch; Residue in the collection screen; The water that adds 10 times of volumes, in 40 ℃ with filter residue agitator treating 10 minutes, cross the leaching filter residue with double gauze; Add the water of 10 times of filter residue volumes in the filter residue, sonicated 30 minutes obtains solution;
Step 2, the pH value of regulator solution is 8.0, adds thermally-stabilised alpha amylase, makes diastatic mass fraction reach 1 ‰, 100 ℃ of following 1200rpm stirred enzymolysis 30 minutes;
Step 3, the solution to 55 that cooling step two finally obtains ℃ adds alkaline papain, makes the mass fraction of protease reach 2 ‰, and 1200rpm stirred enzymolysis 30 minutes; Add lipase afterwards, make its mass fraction reach 2 ‰, 1200rpm stirred enzymolysis 30 minutes;
Step 4, the pH value of the solution that step 3 is finally obtained is adjusted to 9, adds volume fraction and be 7% hydrogen peroxide solution, and 30 ℃ were stirred 30 minutes;
Step 5, the solution that filtration step four finally obtains gets filter residue, and washing is to neutral, and vacuum freeze drying is pulverized, and the filter residue that drying is good is pulverized 100 orders with common pulverizer, pulverizes 400 orders with micronizer then, obtains canna edulis ker polysaccharide polyphenol complex.
Embodiment 3
Step 1, canna edulis ker waste residue come from Guizhou Zi Yunjia and Chemical Industry Science Co., Ltd, with canna edulis ker waste residue in 55 ℃ of dryings; Pulverize, cross 40 mesh sieves, cross 160 mesh sieves afterwards to remove partial starch; Residue in the collection screen; The water that adds 8 times of volumes, in 25 ℃ with filter residue agitator treating 10 minutes, cross the leaching filter residue with double gauze; Add the water of 5 times of filter residue volumes in the filter residue, sonicated 15 minutes obtains solution;
Step 2, the pH value of regulator solution is 7.5, adds thermally-stabilised AMS, makes diastatic mass fraction reach 0.7 ‰, 96 ℃ of following enzymolysis 45 minutes;
Step 3, the solution to 50 that cooling step two finally obtains ℃ adds alkaline papain, makes the mass fraction of protease reach 1.5 ‰, enzymolysis 45 minutes; Add lipase afterwards, make its mass fraction reach 1.5 ‰, enzymolysis 45 minutes;
Step 4, the pH value of the solution that step 3 is finally obtained is adjusted to 10, adds volume fraction and be 6% hydrogen peroxide solution, and 20 ℃ were stirred 45 minutes;
Step 5, the solution that filtration step four finally obtains gets filter residue, and washing is to neutral, and 50 ℃ of drying under reduced pressure 20 hours are pulverized, and obtain canna edulis ker polysaccharide polyphenol complex.
Claims (5)
1. the preparation method of a canna edulis ker polysaccharide polyphenol complex is characterized in that, comprises the steps:
Step 1, the preparation canna edulis ker waste residue adds the water mixing and obtains solution;
Step 2, the pH value of regulator solution is 7.5-8.5, adds thermally-stabilised AMS, makes diastatic mass fraction reach 0.5-1 ‰, 95-100 ℃ following enzymolysis 30-60 minute;
Step 3, cooling solution add alkaline papain to 50-60 ℃, make the mass fraction of protease reach 1-2 ‰, enzymolysis 30-60 minute; Add lipase afterwards, make its mass fraction reach 1-2 ‰, enzymolysis 30-60 minute;
Step 4 is adjusted to 9-11 with the pH value of solution, and adding volume parts is the hydrogen peroxide solution of 4-7%, and 20-30 ℃ was stirred 30-60 minute;
Step 5, filtering solution gets filter residue, and washing is to neutral, and drying under reduced pressure or vacuum freeze drying are pulverized, and obtain canna edulis ker polysaccharide polyphenol complex.
2. the preparation method of canna edulis ker polysaccharide polyphenol complex according to claim 1 is characterized in that, in the step 1; Said preparation is specially: canna edulis ker waste residue in 50-60 ℃ of drying, was pulverized 40 mesh sieves, cross 160 mesh sieves afterwards; 25-40 ℃ of following water washing and filtering gets filter residue.
3. the preparation method of canna edulis ker polysaccharide polyphenol complex according to claim 1 is characterized in that, in the step 1, and said being mixed into sonicated 10-30 minute.
4. the preparation method of canna edulis ker polysaccharide polyphenol complex according to claim 1 is characterized in that, in the step 1, and said solution, the mass ratio of water and filter residue is (5-10) in the solution: 1.
5. the preparation method of canna edulis ker polysaccharide polyphenol complex according to claim 1 is characterized in that, in the step 5, said drying under reduced pressure is to carry out under 40-50 ℃.
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| CN102161706B (en) * | 2011-03-01 | 2013-04-10 | 中国科学院过程工程研究所 | Comprehensive utilization method of Canna edulis Ker tuber |
| CN104292356A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Method for extracting peony polysaccharide from peony cakes with biological enzyme method |
| CN106723040A (en) * | 2016-12-27 | 2017-05-31 | 武汉轻工大学 | A kind of lotus root polyphenol polysaccharide-liposome complex liquid and preparation method thereof |
| CN109380711A (en) * | 2017-08-08 | 2019-02-26 | 贵州美人芋农业发展有限公司 | A kind of method that banana dasheen bar extracts plant sugar |
| CN108619001B (en) * | 2018-06-25 | 2021-09-07 | 上海交通大学医学院 | Red ginseng mask without preservative and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1661028A (en) * | 2004-12-22 | 2005-08-31 | 华南理工大学 | Method for preparing porous starch through enzyme method in high temperature |
| CN1696295A (en) * | 2005-05-26 | 2005-11-16 | 贵州大学 | Method for producing alcohol by using dry plate of banana dasheen as raw material |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1661028A (en) * | 2004-12-22 | 2005-08-31 | 华南理工大学 | Method for preparing porous starch through enzyme method in high temperature |
| CN1696295A (en) * | 2005-05-26 | 2005-11-16 | 贵州大学 | Method for producing alcohol by using dry plate of banana dasheen as raw material |
Non-Patent Citations (1)
| Title |
|---|
| 刘桂香等.双酶法芭蕉芋糖化工艺研究.《食品与工业发酵》.2003,第29卷(第9期),第104-105和110页. * |
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