CN104223125A - Method for preparing DF (Dietary Fiber) of potato pulp - Google Patents
Method for preparing DF (Dietary Fiber) of potato pulp Download PDFInfo
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- CN104223125A CN104223125A CN201410542983.1A CN201410542983A CN104223125A CN 104223125 A CN104223125 A CN 104223125A CN 201410542983 A CN201410542983 A CN 201410542983A CN 104223125 A CN104223125 A CN 104223125A
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- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 140
- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 140
- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 57
- 238000000034 method Methods 0.000 title claims abstract description 42
- 229920002472 Starch Polymers 0.000 claims abstract description 29
- 239000007787 solid Substances 0.000 claims abstract description 29
- 239000008107 starch Substances 0.000 claims abstract description 29
- 235000019698 starch Nutrition 0.000 claims abstract description 29
- 102000004190 Enzymes Human genes 0.000 claims abstract description 22
- 108090000790 Enzymes Proteins 0.000 claims abstract description 22
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 4
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 4
- 239000002893 slag Substances 0.000 claims description 78
- 239000002002 slurry Substances 0.000 claims description 29
- 239000000047 product Substances 0.000 claims description 27
- 108010059892 Cellulase Proteins 0.000 claims description 25
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 238000002156 mixing Methods 0.000 claims description 23
- 230000007062 hydrolysis Effects 0.000 claims description 22
- 238000006460 hydrolysis reaction Methods 0.000 claims description 22
- 229940088598 enzyme Drugs 0.000 claims description 21
- 238000000926 separation method Methods 0.000 claims description 20
- 230000008569 process Effects 0.000 claims description 19
- 239000006228 supernatant Substances 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 15
- 102000004139 alpha-Amylases Human genes 0.000 claims description 14
- 108090000637 alpha-Amylases Proteins 0.000 claims description 14
- 229940024171 alpha-amylase Drugs 0.000 claims description 14
- 239000008103 glucose Substances 0.000 claims description 14
- 238000001035 drying Methods 0.000 claims description 12
- 238000010298 pulverizing process Methods 0.000 claims description 12
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 8
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 8
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 8
- 150000003839 salts Chemical class 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 7
- 108090000145 Bacillolysin Proteins 0.000 claims description 5
- 102000035092 Neutral proteases Human genes 0.000 claims description 5
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- 238000001704 evaporation Methods 0.000 claims description 4
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- 239000000126 substance Substances 0.000 claims description 3
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- 230000007935 neutral effect Effects 0.000 claims description 2
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- 229920002678 cellulose Polymers 0.000 abstract description 12
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- 238000004519 manufacturing process Methods 0.000 abstract description 10
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- 102000013142 Amylases Human genes 0.000 abstract description 8
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- 239000002699 waste material Substances 0.000 abstract description 4
- 108010059820 Polygalacturonase Proteins 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract description 3
- 108010093305 exopolygalacturonase Proteins 0.000 abstract description 3
- 229920001592 potato starch Polymers 0.000 abstract 2
- 239000000835 fiber Substances 0.000 description 17
- 235000005911 diet Nutrition 0.000 description 16
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- 230000007613 environmental effect Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
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- 206010010774 Constipation Diseases 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 108010089934 carbohydrase Proteins 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/03—Products from fruits or vegetables; Preparation or treatment thereof consisting of whole pieces or fragments without mashing the original pieces
- A23L19/07—Fruit waste products, e.g. from citrus peel or seeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for preparing the DF (Dietary Fiber) of potato pulp, and belongs to the field of agriculture products processing waste comprehensive utilization technique. The method comprises the steps of tasking wet potato pulp as raw materials, adopting the structures of destroyed cellulose and pectin components processed by cellulose and pectinase, so as to release the covering and twisting function on starch by the cellulose and the pectin and improve the content of soluble dietary fibers; adding protease to remove protein; adding amylase and saccharifying enzyme to remove starch with high efficiency; preparing a product made from soluble dietary fibers with high added values; preparing a product made from dietary fibers by solid matters, therefore the efficient comprehensive utilization of potato pulp can be realized, the problems that waste residues can be difficultly processed during the production of the potato starch can be effectively solved, the production cost of a potato starch enterprise can be reduced, and an environmental pollution problem caused by a plurality of accumulated potato pulp can be alleviated.
Description
Technical field
The present invention relates to a kind of method preparing potato residues dietary fiber, especially a kind of efficiency utilization potato residues that wets transforms the method for production high added value diet fiber product, belongs to processing of farm products waste ' s reclamation technical field.
Background technology
China is potato planting state maximum in the world, along with the development of China's farina industry, has a large amount of potatos to be processed to starch every year, produces the fresh potato slag of up to a million tons simultaneously.Potato residues is a kind of mixture being rich in multiple nutrients thing, but in fresh slag, moisture is high and containing a large amount of microorganism, very easily putrid and deteriorated, causes environmental pollution.Therefore, develop a kind of effective comprehensive utilization mode of fresh potato slag, to the development of farina industry with solve increasingly serious environmental problem all there is important value.
In potato residues, dietary fiber content is up to 50-60%, and wherein soluble dietary fiber accounts for 14-15%, is a kind of dietary fibre materials of high-quality.The physiological function of dietary fiber comprises Constipation and colon cancer, adjusting blood lipid, adjustment blood sugar and fat-reducing etc.Along with the degree of concern of people to health is more and more higher, the demand of dietary fiber is also increasing.Therefore, with potato residues be raw material prepare dietary fiber will become potato residues develop a kind of effective means.
Existing take agricultural wastes as the technique that material, enzyme method prepares dietary fiber, mainly for raw material that content of starch is lower, moisture is lower such as bean dregs, wheat bran, maize peels, adopt amylase, Protease Treatment to remove impurity, then improve dietary fiber component by cellulase process or improve soluble dietary fibre content.But moisture in wet potato residues, content of starch are all very high, and starch is wrapped in pectin, cellulosic component closely, is difficult to effectively except this main interfering component of desizing.The dietary fiber preparation technology for potato residues developed at present, all dry and pulverization process are carried out to potato residues before treatment, the structure of this process to potato residues creates certain destruction, be conducive to starch release and by enzymolysis, but the retention ability of potato residues own is extremely strong, drying needs energy consumption and the time of at substantial, cannot adapt to the demand that farina processing enterprise produces wet potato residues continuously, in enormous quantities, and starch residual rate is still very high; For the dietary fiber preparation technology of wet potato residues, the removal efficiency of amylorrhexis is very low, too high starch residual amount have impact on the quality of potato residues dietary fiber, limit its application, price is lower, and lack the product type of this high added value of soluble dietary fiber, high-quality, also cause being that the comprehensive utilization technique that dietary fiber prepared by raw material cannot be promoted and extensive use with potato residues.
The present invention is according to the component structure of potato residues uniqueness and feature, targetedly by first adopting cellulase and pectase to degrade in advance, recycling amylase is except desizing, utilize the potato residues soluble dietary fiber that starch residual amount is low to prepare, quality is high and full diet fiber product, the fields such as varieties of food items, beverage, health products and animal feed components can be widely used in, to the development of farina industry with solve increasingly serious environmental problem all there is important value according to different user demand.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of method preparing potato residues dietary fiber, is a kind of method preparing high added value diet fiber product for potato residues component and architectural feature.By adopting cellulase and pectase Combined Processing both can Partial digestion cellulose, it is made to be converted into soluble dietary fiber, cellulose and pectin can be removed again to the parcel constraint effect of starch, improve the efficiency that amylase removes starch, prepare full diet fiber product.Realize the efficiency utilization of potato residues resource, solve the problem of potato residues process difficulty.
The key step of described method is after being mixed with suitable quantity of water by potato residues, and interpolation cellulase and pectase carry out Combined Processing, then use neutral proteinase, Thermostable α-Amylase and α-Isosorbide-5-Nitrae-glucose hydrolysis ferment treatment respectively; The separation of the potato slag obtained after process slurry is obtained supernatant and solid content, potato slag slurry is separated the solid content obtained and is potato residues dietary fiber, and the solid content that the supernatant that the separation of potato slag slurry obtains obtains after concentrated alcohol precipitation is potato residues soluble dietary fiber.
In one embodiment of the invention, described potato residues mixes with suitable quantity of water, and be 1:3 ~ 1:5 (g:mL) mixing by fresh potato slag and water by mass volume ratio, the speed of 100 ~ 200r/min stirs.
In one embodiment of the invention, when utilizing cellulase and pectase to carry out Combined Processing, enzymatic hydrolysis condition is: temperature 50 ~ 55 DEG C, pH4.5 ~ 5.0, time 1 ~ 3h, mixing speed 150 ~ 200r/min, cellulase addition 4 ~ 36U/g potato slag (butt), pectase addition 5 ~ 20U/g potato slag (butt), boil 10min after enzymolysis terminates, be cooled to 55 ~ 60 DEG C.
In one embodiment of the invention, neutral protease enzymolysis is added in the potato slag slurry after cellulase and pectase Combined Processing, enzymatic hydrolysis condition adopts: temperature 55 ~ 60 DEG C, pH5.0 ~ 7.0, time 0.5 ~ 1h, mixing speed 150 ~ 200r/min, addition 30 ~ 50U/g potato slag (butt) of described neutral proteinase.
In one embodiment of the invention, 10 ~ 30min is boiled in potato slag slurry heating after neutral protein ferment treatment and makes the abundant gelatinization of the starch contained by it, adjust ph is 5.5 ~ 6.0, add Thermostable α-Amylase, 90 ~ 95 DEG C, stir hydrolysis 10 ~ 40min under 150 ~ 200r/min, be cooled to 50 ~ 60 DEG C; Adjust ph is 4.0 ~ 4.5, add α-1,4-glucose hydrolysis enzyme, 50 ~ 65 DEG C, stir hydrolysis 1 ~ 2h under 150 ~ 200r/min condition, addition 100 ~ 150U/g potato slag (butt) of described Thermostable α-Amylase, addition 200 ~ 300U/g potato slag (butt) of α-Isosorbide-5-Nitrae-glucose hydrolysis enzyme.
In one embodiment of the invention, potato slag slurry separation after enzymolysis processing is obtained supernatant and solid content, supernatant adds the alcohol settling 4 ~ 24h of 95% of 2-6 times of volume after evaporation and concentration, again through Separation of Solid and Liquid, the solid content drying obtained, pulverizing, excessively 100 ~ 200 mesh sieves, namely obtain finished product potato residues soluble dietary fiber, the solid content drying that the separation of potato slag slurry obtains, pulverizing, excessively 100 ~ 200 mesh sieves, namely obtain finished product potato residues dietary fiber.
One embodiment of the present invention mainly comprise the steps:
(1) by fresh potato slag and pure water mixing, wherein the mass volume ratio of fresh potato slag and water is 1:3 ~ 1:5 (g:mL), and the speed of 100 ~ 200r/min stirs;
(2) cellulase and pectase is utilized to carry out Combined Processing, enzymatic hydrolysis condition is: temperature 50 ~ 55 DEG C, pH4.5 ~ 5.0, time 1 ~ 3h, mixing speed 150 ~ 200r/min, cellulase addition 4 ~ 36U/g potato slag (butt), pectase addition 5 ~ 20U/g potato slag (butt), boil 10min after enzymolysis terminates, be cooled to 55 ~ 60 DEG C;
(3) in the potato slag slurry after step (2) process, neutral protease enzymolysis is added, enzymatic hydrolysis condition: temperature 55 ~ 60 DEG C, pH5.0 ~ 7.0, time 0.5 ~ 1h, mixing speed 150 ~ 200r/min, addition 30 ~ 50U/g potato slag (butt) of described neutral proteinase;
(4) 10 ~ 30min is boiled in the potato slag slurry heating after step (3) process and make the abundant gelatinization of the starch contained by it, be 5.5 ~ 6.0 by NaOH adjust ph, add Thermostable α-Amylase, 90 ~ 95 DEG C, stir hydrolysis 10 ~ 40min under 150 ~ 200r/min, be cooled to 50 ~ 60 DEG C; Be 4.0 ~ 4.5 with salt acid for adjusting pH value, add α-1,4-glucose hydrolysis enzyme, 50 ~ 65 DEG C, stir hydrolysis 1 ~ 2h under 150 ~ 200r/min condition, addition 100 ~ 150U/g potato slag (butt) of described Thermostable α-Amylase, addition 200 ~ 300U/g potato slag (butt) of α-Isosorbide-5-Nitrae-glucose hydrolysis enzyme;
(5) the potato slag slurry separation after step (4) process is obtained supernatant and solid content, supernatant adds the alcohol settling 4 ~ 24h of 95% of 2-6 times of volume after evaporation and concentration, again through Separation of Solid and Liquid, the solid content drying obtained, pulverizing, excessively 100 ~ 200 mesh sieves, namely finished product potato residues soluble dietary fiber is obtained, the solid content drying that the separation of potato slag slurry obtains, pulverizing, excessively 100 ~ 200 mesh sieves, namely obtain finished product potato residues dietary fiber.
In one embodiment of the invention, described raw material potato residues is the direct accessory substance of farina processing, and water content is 80 ~ 95%.
Described cellulase is made up of circumscribed 1,4 beta-glucanase, Endo-β-glucanase and beta-glucosidase.
The separation method of described enzymolysis product can be centrifugal or isolated by filtration.
Compared with prior art, tool has the following advantages in the present invention:
1, the present invention directly processes for the direct accessory substance potato residues that wets of farina processing, without the need to carrying out drying and crushing operation in advance, has greatly saved energy consumption and time, can realize continuous, large batch of Efficient Conversion and utilize.
2, present invention finds and utilizing potato residues to prepare in the process of dietary fiber, starch ingredients is comparatively closely wrapped in cellulose and pectin fraction, is difficult to by amylase directly effectively removing thus reduce the problem of dietary fiber quality.The present invention destroys the structural property of cellulose and pectin to a certain extent by first adding cellulase and pectase, then adds amylase and remove starch, can improve starch clearance largely, obtain the low-down dietary fiber of starch residual amount.
3, the present invention adopts cellulase and pectinase treatment, degraded cellulose and pectin to a certain extent, not only can remove cellulose and pectin to the parcel of starch, insoluble diedairy fiber can also be changed into soluble dietary fiber, increase the content of soluble dietary fiber, improve the quality of dietary fiber.
4, the present invention finds that cellulosic component in potato residues is for other agricultural product castoffs, and structure is more loose, and more easily by cellulose hydrolyzation, and pectin fraction content is higher, is a kind of ideal raw material preparing soluble dietary fiber.The present invention has prepared potato residues soluble dietary fiber and full diet fiber two kinds of products simultaneously, can meet different field, the different application needs required, achieve the high-efficiency comprehensive utilization to potato residues.
Detailed description of the invention
Embodiment 1
Take fresh potato slag 100g, be 1:5 (g:mL) by the mass volume ratio of fresh potato slag and water, add water and stir, salt acid for adjusting pH value is 4.8, add the cellulase (Sigma production code member 22178) of 12U/g potato slag (butt) and the pectase (Sigma production code member P5400) of 8U/g potato slag (butt), in 50 DEG C, react 3h under the condition of mixing speed 160r/min, boil, be cooled to 60 DEG C; NaOH adjust ph is 6.0, adds neutral proteinase (Sigma production code member C2139) enzymolysis, enzyme concentration 35U/g potato slag (butt), in 60 DEG C, react 0.5h under the condition of mixing speed 160r/min; By the potato slag slurry after above-mentioned process, 15min is boiled in heating makes the abundant gelatinization of starch, be 5.5 by NaOH adjust ph, add Thermostable α-Amylase (Novi letter Fungamyl800L), enzyme concentration 100U/g potato slag (butt), in 95 DEG C, the Water Under solution 0.5h of mixing speed 160r/min, be cooled to 60 DEG C, be 4.0 with salt acid for adjusting pH value, add α-1,4-glucose hydrolysis enzyme (Novi letter AmylaseAG300L), enzyme concentration 200U/g potato slag (butt), in 60 DEG C, the Water Under solution 1.5h of mixing speed 160r/min.By the potato slag slurry after above-mentioned process with the centrifugation 20min of 4500r/min, be separated and obtain supernatant and solid content.Supernatant, after rotary evaporation is concentrated, adds the alcohol settling 12h of 95% of 3.5 times of volumes, then is separated with the centrifugation 20min of 4500r/min, and the solid content drying obtained, pulverizing, excessively 100 mesh sieves, namely obtain finished product soluble potato dregs diet fibre.Full diet fiber product is obtained after the solid content drying that the separation of potato slag slurry obtains, pulverizing, excessively 100 mesh sieves.
Obtained soluble dietary fiber yield is 41.7%, and full diet fiber yield is 16.4%, and adopting GB5009.9-85 to measure starch residual amount in product normal potato dregs diet fibre is 3.37%.
Embodiment 2
Dietary fiber preparation method 1: take fresh potato slag 100g, be 1:5 by the mass volume ratio of fresh potato slag and water, add water and stir, salt acid for adjusting pH value is 5.0, add the cellulase (Novi letter model C elluzyme) of 25U/g potato slag (butt) and the pectase (Sigma production code member P5400) of 5U/g potato slag (butt), in 50 DEG C, react 2h under the condition of mixing speed 180r/min, boil, be cooled to 60 DEG C; NaOH adjust ph is 6.0, adds neutral proteinase (Sigma production code member C2139) enzymolysis, enzyme concentration 35U/g potato slag (butt), in 60 DEG C, react 0.5h under the condition of mixing speed 200r/min; By the potato slag slurry after above-mentioned process, 20min is boiled in heating makes the abundant gelatinization of starch, add Thermostable α-Amylase (Novi letter FungamYl800L), enzyme concentration 130U/g potato slag (butt), in 95 DEG C, the Water Under solution 0.5h of mixing speed 160r/min, be cooled to 60 DEG C, be 4.0 with salt acid for adjusting pH value, add α-1,4-glucose hydrolysis enzyme (Novi letter AmylaseAG300L), enzyme concentration 200U/g potato slag (butt), in 60 DEG C, the Water Under solution 1.5h of mixing speed 160r/min.All the other steps are with embodiment 1.Obtained soluble dietary fiber yield is 44.3%, and full diet fiber yield is 14.1%.
Dietary fiber preparation method 2: take fresh potato slag 100g, be 1:5 by the mass volume ratio of fresh potato slag and water, add water and stir, 20min is boiled in heating makes the abundant gelatinization of starch, salt acid for adjusting pH value is 6.0, add Thermostable α-Amylase (Novi letter FungamYl800L), enzyme concentration 220U/g potato slag (butt), in 95 DEG C, the Water Under solution 3.5h of mixing speed 160r/min, be cooled to 60 DEG C, be 4.0 with salt acid for adjusting pH value, add α-1, 4-glucose hydrolysis enzyme, enzyme concentration 3000U/g potato slag (butt), in 60 DEG C, the Water Under solution 0.5h of mixing speed 160r/min, add neutral protease enzymolysis, enzyme concentration 50U/g potato slag (butt), in 60 DEG C, react 0.5h under the condition of mixing speed 200r/min, potato residues diet fiber product is obtained by after centrifugal, dry for the potato slag of gained slurry, pulverizing, excessively 100 mesh sieves.The full diet fiber yield of gained is 55.4%
In the present embodiment, dietary fiber preparation method 1,2 products obtained therefrom character is more as shown in table 1:
Table 1 potato residues diet fiber product compares
Compared with preparation method 2, preparation method 1, namely the inventive method is by first cellulase, pectinase treatment, rear protease, amylase process, prepare soluble dietary fiber and full diet fiber simultaneously, further, in diet fiber product, starch residual amount is reduced to 2.09% by 20.63%, holds rate and OH free radical scavenging activity also significantly improves.
In sum, the present invention with wet potato residues for raw material, first cellulase and the destruction cellulose of pectase Combined Processing and the structure of pectin fraction is adopted, both cellulose and pectin had been removed to the parcel of starch, winding effect, the content of soluble dietary fiber can be improved again, then add protease and remove protein, add amylase again and carbohydrase removes starch expeditiously, supernatant after separation can prepare the soluble dietary fiber product of high added value, solid content can prepare the diet fiber product of low content of starch, achieve the high-efficiency comprehensive utilization to potato residues, effectively can solve the reluctant problem of waste residue in farina production, reduce the production cost of farina enterprise, alleviate the problem of environmental pollution that potato slag bulk deposition causes.
Though the present invention with preferred embodiment openly as above; but it is also not used to limit the present invention, any person skilled in the art, not departing from spirit and scope of the invention; all can do various changes and modification, what therefore protection scope of the present invention should define with claims is as the criterion.
Claims (10)
1. prepare a method for potato residues dietary fiber, after being mixed with suitable quantity of water by potato residues, interpolation cellulase and pectase carry out Combined Processing, then use neutral proteinase, Thermostable α-Amylase and α-Isosorbide-5-Nitrae-glucose hydrolysis ferment treatment respectively; The potato slag slurry obtained after process is separated the solid content obtained and is potato residues dietary fiber, and the solid content that the supernatant that the separation of potato slag slurry obtains obtains after concentrated alcohol precipitation is potato residues soluble dietary fiber.
2. method according to claim 1, is characterized in that, described potato residues mixes with suitable quantity of water, is be 1:3 ~ 1:5kg/L mixing by fresh potato slag and water by mass volume ratio, stirs.
3. method according to claim 1, it is characterized in that, cellulase and pectase is utilized to carry out Combined Processing, enzymatic hydrolysis condition is: temperature 50 ~ 55 DEG C, pH4.5 ~ 5.0, time 1 ~ 3h, mixing speed 150 ~ 200r/min, cellulase addition 4 ~ 36U/g potato slag butt, pectase addition 5 ~ 20U/g potato slag butt, boils 10min after enzymolysis terminates, is cooled to 55 ~ 60 DEG C.
4. according to the arbitrary described method of claim 1-3, it is characterized in that, neutral protease enzymolysis is added in the potato slag slurry after cellulase and pectase Combined Processing, enzymatic hydrolysis condition adopts: temperature 55 ~ 60 DEG C, pH5.0 ~ 7.0, time 0.5 ~ 1h, mixing speed 150 ~ 200r/min, addition 30 ~ 50U/g potato slag of described neutral proteinase.
5. method according to claim 4, it is characterized in that, 10 ~ 30min is boiled in potato slag slurry heating after cellulase, pectase, neutral protein ferment treatment and makes the abundant gelatinization of the starch contained by it, adjust ph is 5.5 ~ 6.0, add Thermostable α-Amylase, 90 ~ 95 DEG C, stir hydrolysis 10 ~ 40min under 150 ~ 200r/min, be cooled to 50 ~ 60 DEG C; Adjust ph is 4.0 ~ 4.5, add α-1,4-glucose hydrolysis enzyme, 50 ~ 65 DEG C, stir hydrolysis 1 ~ 2h under 150 ~ 200r/min condition, addition 100 ~ 150U/g potato slag butt of described Thermostable α-Amylase, addition 200 ~ 300U/g potato slag butt of α-Isosorbide-5-Nitrae-glucose hydrolysis enzyme.
6. according to the arbitrary described method of claim 1-3, it is characterized in that, potato slag slurry separation after enzymolysis processing being terminated obtains supernatant and solid content, supernatant adds the alcohol settling 4 ~ 24h of 95% of 2-6 times of volume after evaporation and concentration, again through Separation of Solid and Liquid, the solid content drying obtained, pulverizing, excessively 100 ~ 200 mesh sieves, namely obtain finished product potato residues soluble dietary fiber; The solid content drying that the separation of potato slag slurry obtains, pulverizing, excessively 100 ~ 200 mesh sieves, namely obtain finished product potato residues dietary fiber.
7., according to the arbitrary described method of claim 1-3, it is characterized in that, concrete steps are:
(1) by fresh potato slag and pure water mixing, wherein the mass volume ratio of fresh potato slag and water is that the speed of 1:3 ~ 1:5kg/L, 100 ~ 200r/min stirs;
(2) cellulase and pectase is utilized to carry out Combined Processing, enzymatic hydrolysis condition is: temperature 50 ~ 55 DEG C, pH4.5 ~ 5.0, time 1 ~ 3h, mixing speed 150 ~ 200r/min, cellulase addition 4 ~ 36U/g potato slag, pectase addition 5 ~ 20U/g potato slag, boil 10min after enzymolysis terminates, be cooled to 55 ~ 60 DEG C;
(3) in the potato slag slurry after step (2) process, neutral protease enzymolysis is added, enzymatic hydrolysis condition: temperature 55 ~ 60 DEG C, pH5.0 ~ 7.0, time 0.5 ~ 1h, mixing speed 150 ~ 200r/min, addition 30 ~ 50U/g potato slag of described neutral proteinase;
(4) 10 ~ 30min is boiled in the potato slag slurry heating after step (3) process and make the abundant gelatinization of the starch contained by it, be 5.5 ~ 6.0 by NaOH adjust ph, add Thermostable α-Amylase, 90 ~ 95 DEG C, stir hydrolysis 10 ~ 40min under 150 ~ 200r/min, be cooled to 50 ~ 60 DEG C; Be 4.0 ~ 4.5 with salt acid for adjusting pH value, add α-Isosorbide-5-Nitrae-glucose hydrolysis enzyme, 50 ~ 65 DEG C, stir hydrolysis 1 ~ 2h under 150 ~ 200r/min condition, addition 100 ~ 150U/g potato slag of described Thermostable α-Amylase, addition 200 ~ 300U/g potato slag of α-Isosorbide-5-Nitrae-glucose hydrolysis enzyme;
(5) the potato slag slurry separation after step (4) process is obtained supernatant and solid content, supernatant adds the alcohol settling 4 ~ 24h of 95% of 2-6 times of volume after evaporation and concentration, again through Separation of Solid and Liquid, the solid content drying obtained, pulverizing, excessively 100 ~ 200 mesh sieves, namely finished product potato residues soluble dietary fiber is obtained, the solid content drying that the separation of potato slag slurry obtains, pulverizing, excessively 100 ~ 200 mesh sieves, namely obtain finished product potato residues dietary fiber.
8. method according to claim 1, is characterized in that, described raw material potato residues is the direct accessory substance of farina processing, and water content is 80 ~ 95%.
9. method according to claim 1, is characterized in that, described cellulase is made up of circumscribed 1,4 beta-glucanase, Endo-β-glucanase and beta-glucosidase.
10. recycling a method for potato residues, is after being mixed with suitable quantity of water by potato residues, and interpolation cellulase and pectase carry out Combined Processing, then use neutral proteinase, Thermostable α-Amylase and α-Isosorbide-5-Nitrae-glucose hydrolysis ferment treatment respectively; The separation of the potato slag obtained after process slurry is obtained supernatant and solid content, potato slag slurry is separated the solid content obtained and is potato residues dietary fiber, and the solid content that the supernatant that the separation of potato slag slurry obtains obtains after concentrated alcohol precipitation is potato residues soluble dietary fiber.
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