CN107960665A - A kind of method using potato starchy residue extraction dietary fiber - Google Patents
A kind of method using potato starchy residue extraction dietary fiber Download PDFInfo
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- CN107960665A CN107960665A CN201711444167.7A CN201711444167A CN107960665A CN 107960665 A CN107960665 A CN 107960665A CN 201711444167 A CN201711444167 A CN 201711444167A CN 107960665 A CN107960665 A CN 107960665A
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- China
- Prior art keywords
- dietary fiber
- potato
- mixed liquor
- method described
- enzymolysis product
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- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 32
- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 32
- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000000605 extraction Methods 0.000 title claims abstract description 12
- 239000000047 product Substances 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 230000001954 sterilising effect Effects 0.000 claims abstract description 17
- 238000006243 chemical reaction Methods 0.000 claims abstract description 11
- 239000000413 hydrolysate Substances 0.000 claims abstract description 11
- 238000001976 enzyme digestion Methods 0.000 claims abstract description 10
- 108010059892 Cellulase Proteins 0.000 claims abstract description 8
- 229940106157 cellulase Drugs 0.000 claims abstract description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 8
- 230000007062 hydrolysis Effects 0.000 claims abstract description 6
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 6
- 102000004139 alpha-Amylases Human genes 0.000 claims abstract description 4
- 108090000637 alpha-Amylases Proteins 0.000 claims abstract description 4
- 108090000790 Enzymes Proteins 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 229940088598 enzyme Drugs 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 5
- 229940111205 diastase Drugs 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 3
- 235000012054 meals Nutrition 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 230000035484 reaction time Effects 0.000 claims 1
- 229940024171 alpha-amylase Drugs 0.000 abstract description 2
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- 239000000835 fiber Substances 0.000 description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 229920002472 Starch Polymers 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 235000019698 starch Nutrition 0.000 description 6
- 239000008107 starch Substances 0.000 description 6
- 230000014759 maintenance of location Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000008901 benefit Effects 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000002893 slag Substances 0.000 description 3
- 235000011121 sodium hydroxide Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- 238000003912 environmental pollution Methods 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011953 bioanalysis Methods 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000009144 enzymatic modification Effects 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
- A23L33/22—Comminuted fibrous parts of plants, e.g. bagasse or pulp
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention discloses a kind of method using potato starchy residue extraction dietary fiber, includes the following steps 1) to mix potato starchy residue and water, obtains mixed liquor；2) pH of the mixed liquor is adjusted to 4.5 4.8, then by the mixing of mixed liquor, pectase and cellulase, and enzyme digestion reaction is carried out in water bath with thermostatic control, obtain enzymolysis product；3) pH of the enzymolysis product is adjusted to 5.0 6.0, then by enzymolysis product and the mixing of medium temperature alpha amylase, and carries out hydrolysis, obtain hydrolysate；4) hydrolysate is subjected to sterilization treatment, is concentrated, centrifuged after sterilizing, washed and dry dietary fiber.Examined by GB5009 and GB4789 methods using the sanitary index of the method for the present invention extraction dietary fiber, met the requirements.Simple process and low cost at the same time, makes potato residue be fully used, reduces the pollution to environment.It is easy to large-scale production.
Description
Technical field
The invention belongs to biological technical field.Potato starchy residue extraction dietary fiber is utilized more particularly, to one kind
Method.
Background technology
Potato starchy residue is a kind of accessory substance of the remaining starch particle produced in farina production process.Its egg
Bai Hanliang is low, and crude fiber content is high, and since production season concentrates on summer and autumn, temperature is high, therefore should not store, transport.Largely
Potato slag muck product, if handle not in time within these few days will corruption produce stench, cause environmental pollution.If drying and processing, energy consumption
It is very high.Therefore, how using most economical method processing potato slag, environmental pollution is eliminated, while be allowed to produce certain economy
Benefit.
The effective way for solving potato residues bulk deposition is exactly it is converted into a kind of value product.With potato
Slag is raw material, and soluble dietary fiber of the production with physiological function, so can significantly improve its utilization rate, widen horse
Bell potato secondary industry chain, is greatly improved the economic benefit of China's Potato Industry.
At present, being mainly modified potato insoluble diedairy fiber by chemical method, Physical, bioanalysis etc. allows it to become
Soluble dietary fiber, it is therefore an objective to allow it to become dietary fiber of high-quality, strengthening either to increase newly original not or has but very
Faint functional characteristic, such as:Expansive force, retention ability etc..Alkaline process, the loss of acid system modified dietary fiber in chemical method is big, and
The physiological property of soluble dietary fiber is poor；The soluble dietary fiber of enzyme modification, its physiological property is preferable, but technique is unstable
It is fixed；The method of Physical modified dietary fiber is newer, has the advantages that recovery rate is high, product impurity is few.
The content of the invention
It is an object of the present invention to provide a kind of method using potato starchy residue extraction dietary fiber, including such as
Lower step:
1) potato starchy residue and water are mixed, obtains mixed liquor；
2) pH of the mixed liquor is adjusted to 4.5-4.8, then mixes mixed liquor, pectase and cellulase, and
Enzyme digestion reaction is carried out in water bath with thermostatic control, obtains enzymolysis product；
3) pH of the enzymolysis product is adjusted to 5.0-6.0, then mixes enzymolysis product and mesophilicα-diastase, and
Reaction is hydrolyzed, obtains hydrolysate；
4) hydrolysate is subjected to sterilization treatment, is concentrated, centrifuged after sterilizing, washed and dry meals
Fiber.
Further, the potato starchy residue extracts the by-product after starch for letter Feng Nongye Co., Ltds of Jilin Province potato
Thing；
Further, the mass ratio of the potato starchy residue and water is 1:3-15.5；Preferably, potato starchy residue and water
Mass ratio be 1:5.
Further, it is put into after potato starchy residue and water being mixed in thermostatic control oscillator vibration.
Preferably, the thermostatic control oscillator vibration is SHZ-Type B, purchased from Shanghai Bo Tai experimental facilities Co., Ltd；
Further, the temperature of the enzyme digestion reaction is 40-60 DEG C, and the time of enzyme digestion reaction is 3-5h.
Further, the hydrolysising reacting temperature is 50-60 DEG C, hydrolysis time 1-2h.
Further, the sterilization treatment refers to hydrolysate carrying out sterilizing 15-30min by boiling water bath, is dropped after sterilizing
Warm to room temperature, then concentrated using Rotary Evaporators.
Preferably, the Rotary Evaporators are RE-2000A types, purchased from Shanghai Yarong Biochemical Instrument Plant.
Further, the centrifugation refers to centrifuge 3-10min under conditions of rotating speed is 3500-4600r/min；It is preferred that
Ground, centrifugation time 5min.
Further, it is IT-09A types to centrifuge centrifuge used, purchased from the good limited public affairs of scientific instrument in section in Anhui
Department.
Further, the enzyme activity of the pectase is 40U g, and pectase is purchased from Xia Sheng Industry Group Co., Ltd.
Further, the enzyme activity of the cellulase for 50U g, cellulase is purchased from Sigma-Aldrich groups.
The alpha-amylase is purchased from the extensive and profound in meaning star biotechnology Co., Ltd in Beijing；The enzyme of 3700U/g ＜ alpha-amylases
Vigor ＜ 4800U/g.
Preferably, the drying is that 8-15h is dried under 40 DEG C -60 using electric drying oven with forced convection.
Beneficial effects of the present invention are as follows：
1st, the sanitary index of potato starchy residue extraction dietary fiber is examined by GB5009 and GB4789 methods, is conformed to
Ask；Simple process and low cost at the same time, is easy to large-scale production.
2nd, method of the invention makes potato residue be fully used, and reduces the pollution to environment.
3rd, reduced using the remaining quantity of starch in the dietary fiber of the method for the present invention extraction, retention ability 800%；Work as temperature
For 20 DEG C when, dietary fiber expansive force increase, water-soluble fibre more than 10%, total fiber more than 70%.
Embodiment
In order to illustrate more clearly of the present invention, with reference to preferred embodiment, the present invention is described further.Ability
Field technique personnel should be appreciated that following specifically described content is illustrative and be not restrictive, this should not be limited with this
The protection domain of invention.
Embodiment 1
A kind of method using potato starchy residue extraction dietary fiber, includes the following steps:
1) by potato starchy residue and water in mass ratio 1:5 are uniformly mixed, and are then placed in thermostatic control oscillator vibration, obtain mixed
Close liquid；
2) added into the mixed liquor 1mol L HCL aqueous solutions, its pH is adjusted to 4.5-4.8, then will mixing
Liquid, 40U g pectase and 50U g cellulase mixing, and in 50 DEG C of waters bath with thermostatic control carry out enzyme digestion reaction 4h, must digest
Product；
3) added into the enzymolysis product 1mol L sodium hydrate aqueous solution, its pH is adjusted to 5.0-6.0, then
The mesophilicα-diastase of enzymolysis product and enzyme activity more than 3700U/g is mixed, and in 55 DEG C of hydrolysis 1h, production must be hydrolyzed
Thing；
4) hydrolysate is subjected to sterilization treatment 20min in boiling water bath, room temperature is cooled to after sterilizing, is then used
Rotary Evaporators are concentrated, and use IT-09A types centrifuge that 5min is centrifuged with the rotating speed of 4500r/min, and use matter
Measure the ethanol that fraction is 95% to wash 3 times, then the dry 12h under the conditions of 50 DEG C, obtains dietary fiber.
The remaining quantity of starch is reduced to 1.28% by original 20.63% in the dietary fiber, retention ability 800%；
When temperature is 20 DEG C, dietary fiber expansive force by 5 milliliters of starting through 24 it is small when reach 13 milliliters, water-soluble fibre 15%, always
Fiber 76.4%.
Embodiment 2
A kind of method using potato starchy residue extraction dietary fiber, includes the following steps:
1) by potato starchy residue and water in mass ratio 1:6 are uniformly mixed, and are then placed in thermostatic control oscillator vibration, obtain mixed
Close liquid；
2) added into the mixed liquor 1mol L HCL aqueous solutions, its pH is adjusted to 4.5-4.8, then will mixing
Liquid, 40U g pectase and 50U g cellulase mixing, and in 50 DEG C of waters bath with thermostatic control carry out enzyme digestion reaction 4h, must digest
Product；
3) added into the enzymolysis product 1mol L sodium hydrate aqueous solution, its pH is adjusted to 5.0-6.0, so
The mesophilicα-diastase of enzymolysis product and enzyme activity more than 3700U/g is mixed afterwards, and in 55 DEG C of hydrolysis 1h, production must be hydrolyzed
Thing；
4) hydrolysate is subjected to sterilization treatment 20min in boiling water bath, room temperature is cooled to after sterilizing, is then used
Rotary Evaporators are concentrated, and use IT-09A types centrifuge that 5min is centrifuged with the rotating speed of 4600r/min, and use matter
Measure the ethanol that fraction is 95% to wash 3 times, then the dry 12h under the conditions of 50 DEG C, obtains dietary fiber.
The remaining quantity of starch is reduced to 1.28% by original 20.63% in the dietary fiber, retention ability 800%；
When temperature is 20 DEG C, dietary fiber expansive force by 5 milliliters of starting through 24 it is small when reach 12 milliliters, water-soluble fibre 16.0%,
Total fiber 76.4%.
Embodiment 3
A kind of method using potato starchy residue extraction dietary fiber, includes the following steps:
1) by potato starchy residue and water in mass ratio 1:5 are uniformly mixed, and are then placed in thermostatic control oscillator vibration, obtain mixed
Close liquid；
2) added into the mixed liquor 1mol L HCL aqueous solutions, its pH is adjusted to 4.5-4.8, then will mixing
Liquid, 40U g pectase and 50U g cellulase mixing, and in 50 DEG C of waters bath with thermostatic control carry out enzyme digestion reaction 4h, must digest
Product；
3) added into the enzymolysis product 1mol L sodium hydrate aqueous solution, its pH is adjusted to 5.0-6.0, so
The mesophilicα-diastase of enzymolysis product and enzyme activity more than 3700U/g is mixed afterwards, and reaction 1h is hydrolyzed at 55 DEG C, obtains water
Solve product；
4) hydrolysate is subjected to sterilization treatment 20min in boiling water bath, room temperature is cooled to after sterilizing, is then used
Rotary Evaporators are concentrated, and use IT-09A types centrifuge that 5min is centrifuged with the rotating speed of 4500r/min, and use matter
Measure the ethanol that fraction is 95% to wash 3 times, then the dry 12h under the conditions of 50 DEG C, obtains dietary fiber.
The remaining quantity of starch is reduced to 1.28% by original 20.63% in the dietary fiber, retention ability 800%；
When temperature is 20 DEG C, dietary fiber expansive force by 5 milliliters of starting through 24 it is small when reach 12 milliliters, water-soluble fibre 15.6%,
Total fiber 76.8%.
Obviously, the above embodiment of the present invention is only intended to clearly illustrate example of the present invention, and is not pair
The restriction of embodiments of the present invention, for those of ordinary skill in the field, may be used also on the basis of the above description
To make other variations or changes in different ways, all embodiments can not be exhaustive here, it is every to belong to this hair
Row of the obvious changes or variations that bright technical solution is extended out still in protection scope of the present invention.
Claims (7)
- A kind of 1. method using potato starchy residue extraction dietary fiber, it is characterised in that include the following steps：1) potato starchy residue and water are mixed, obtains mixed liquor；2) pH of the mixed liquor is adjusted to 4.5-4.8, then mixes mixed liquor, pectase and cellulase, and in perseverance Enzyme digestion reaction is carried out in tepidarium, obtains enzymolysis product；3) pH of the enzymolysis product is adjusted to 5.0-6.0, then mixed enzymolysis product and mesophilicα-diastase, and carry out Hydrolysis, obtains hydrolysate；4) hydrolysate is subjected to sterilization treatment, is concentrated, centrifuged after sterilizing, washed and is dry that meals are fine Dimension.
- 2. according to the method described in claim 1, it is characterized in that, the mass ratio of the potato starchy residue and water is 1:3- 15.5。
- 3. according to the method described in claim 1, it is characterized in that, the temperature of the enzyme digestion reaction be 40-60 DEG C, enzyme digestion reaction Time be 3-5h.
- 4. according to the method described in claim 1, it is characterized in that, the hydrolysising reacting temperature is 50-60 DEG C, during hydrolysis Between be 1-2h.
- 5. according to the method described in claim 1, it is characterized in that, the sterilization treatment refers to hydrolysate passing through boiling water bath Carry out sterilizing 15-30min.
- 6. according to the method described in claim 1, it is characterized in that, the centrifugation refers to that in rotating speed be 3500-4600r/ 3-10min is centrifuged under conditions of min.
- 7. according to the method described in claim 1, it is characterized in that, the enzyme activity of the pectase for 40U g, the cellulose The enzyme activity of enzyme for 50U g, the enzyme activity ＜ 4800U/g of the 3700U/g ＜ alpha-amylases.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201711444167.7A CN107960665A (en) | 2017-12-27 | 2017-12-27 | A kind of method using potato starchy residue extraction dietary fiber |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711444167.7A CN107960665A (en) | 2017-12-27 | 2017-12-27 | A kind of method using potato starchy residue extraction dietary fiber |
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| Publication Number | Publication Date |
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| CN107960665A true CN107960665A (en) | 2018-04-27 |
Family
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116023515A (en) * | 2023-02-01 | 2023-04-28 | 集美大学 | Preparation method and application of corn starch sugar residue resistant starch |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101715946A (en) * | 2009-10-31 | 2010-06-02 | 华南理工大学 | Method for extracting dietary fiber from potato residues |
| CN104223125A (en) * | 2014-10-14 | 2014-12-24 | 江南大学 | Method for preparing DF (Dietary Fiber) of potato pulp |
| CN104341532A (en) * | 2013-08-07 | 2015-02-11 | 中国食品发酵工业研究院 | Potato slag comprehensive utilization processing method for combined production of starch and meal fiber |
| CN104365732A (en) * | 2014-11-24 | 2015-02-25 | 中国农业科学院农产品加工研究所 | Bread rich in sweet potato dietary fiber and manufacturing method thereof |
-
2017
- 2017-12-27 CN CN201711444167.7A patent/CN107960665A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101715946A (en) * | 2009-10-31 | 2010-06-02 | 华南理工大学 | Method for extracting dietary fiber from potato residues |
| CN104341532A (en) * | 2013-08-07 | 2015-02-11 | 中国食品发酵工业研究院 | Potato slag comprehensive utilization processing method for combined production of starch and meal fiber |
| CN104223125A (en) * | 2014-10-14 | 2014-12-24 | 江南大学 | Method for preparing DF (Dietary Fiber) of potato pulp |
| CN104365732A (en) * | 2014-11-24 | 2015-02-25 | 中国农业科学院农产品加工研究所 | Bread rich in sweet potato dietary fiber and manufacturing method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116023515A (en) * | 2023-02-01 | 2023-04-28 | 集美大学 | Preparation method and application of corn starch sugar residue resistant starch |
| CN116023515B (en) * | 2023-02-01 | 2024-02-20 | 集美大学 | Preparation method and application of corn starch sugar residue resistant starch |
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Application publication date: 20180427 |