CN104774887A - Preparation method of corncob xylooligosaccharide - Google Patents
Preparation method of corncob xylooligosaccharide Download PDFInfo
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- CN104774887A CN104774887A CN201510170829.0A CN201510170829A CN104774887A CN 104774887 A CN104774887 A CN 104774887A CN 201510170829 A CN201510170829 A CN 201510170829A CN 104774887 A CN104774887 A CN 104774887A
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- xylo
- corn cob
- oligosaccharide
- xylooligosaccharide
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Abstract
The invention relates to a preparation method of corncob xylooligosaccharide. The method comprises the following steps: firstly, crushing corncob employing a superfine grinding technology; carrying out pretreatment on the corncob employing a high-temperature and high-pressure cooking process; hydrolyzing the corncob employing ultrasonic-assisted complex enzyme to prepare xylooligosaccharide; and finally separating and purifying the prepared xylooligosaccharide employing the processes such as decolorizing, desalting, alcohol precipitation and vacuum drying, and further preparing the high-purity xylooligosaccharide. A certain technical support is provided for reduction of the production cost of the xylooligosaccharide and improvement of the industrial production process for preparing the xylooligosaccharide with the corncob.
Description
Technical field
The present invention relates to and belong to food technology field, be specifically related to a kind of preparation method of corn cob xylo-oligosaccharide.
Background technology
Xylo-oligosaccharide has good physiology characteristic, and as the multiplication capacity of bifidus bacillus, not digested characteristic, preventing dental caries, promotes that human calcium absorbs.It is a kind of important functional oligose being widely used in the industry such as food, medicine and makeup.
The preparation of xylo-oligosaccharide, primarily of the plant resources being rich in xylan, if corn cob, cotton seed hulls, Pericarppium arachidis hypogaeae, bagasse and rice husk etc. are raw material, is obtained by methods such as acid hydrolysis, basic hydrolysis, high temperature steaming or enzymic hydrolysiss.China is a large agricultural country, and corn is one of China's Three major grain crops, has abundant corn cob raw material resources.Current China is relatively less to the utilization of corn cob, is substantially in the low-level elementary process segment, and the overwhelming majority is burnt as farmers''s fuel.Be raw material with corn cob, prepare functional low polyxylose, not only can fully utilize agricultural resource, also can increase farmers' income simultaneously, good market outlook will be had.
Summary of the invention
The object of the present invention is to provide a kind of method preparing corn cob xylo-oligosaccharide.
The method that the present invention prepares corn cob xylo-oligosaccharide mainly comprises the techniques such as corn cob micronizing, High Temperature High Pressure pre-treatment, ultrasonic assistant combinative enzyme hydrolysis, decolouring, desalination, alcohol precipitation and drying.
A preparation method for corn cob xylo-oligosaccharide, comprises the following steps:
(1) micronizing
Select drying, be starting material without mouldy corn cob, after being pulverized with supper micron mill, for subsequent use;
(2) High Temperature High Pressure pre-treatment
Add the buffered soln of pH 5.0 ~ 6.0 according to the amount of solid-liquid ratio 1:15 ~ 1:30, be then placed in high-pressure sterilizing pot, at 105 DEG C ~ 120 DEG C temperature, High Temperature High Pressure pre-treatment 30min ~ 50min;
(3) ultrasonic assistant combinative enzyme hydrolysis
The prozyme be made up of according to 3:2 ratio zytase and cellulase of 0.8% ~ 1.0% is added in the pretreated corn cob feed liquid of High Temperature High Pressure, at ultrasonic power 250W ~ 300W, at ultrasonic temperature 50 DEG C ~ 60 DEG C, ultrasonic wave added enzymic hydrolysis 10min ~ 20min;
(4) go out enzyme
Corn cob solution after hydrolysis is incubated 15min at 80 DEG C, then centrifugation, collects supernatant liquor;
(5) decolour
With macroporous resin AB-8 according to 1.5% ~ 2.5% addition, under pH 6.0 and 40 DEG C ~ 45 DEG C temperature, decolouring 1.5h ~ 2h;
(6) desalination
Use D301 and 001 × 7 anion-cation exchange resin according to the addition of 8% ~ 10% respectively, under pH 6.0 and 40 DEG C ~ 50 DEG C temperature, desalination is carried out to xylo-oligosaccharide solution;
(7) alcohol precipitation
Xylo-oligosaccharide solution after desalination, after membrane separation concentration, adds the ethanol of 2 ~ 4 times of volumes, 4 DEG C of standing 12h, filtering solution, collecting precipitation;
(8) dry
Precipitation after filtering is put into vacuum drying oven, after drying, is xylo-oligosaccharide finished product.
The invention has the advantages that: first adopt superfine communication technique to be pulverized by corn cob, in conjunction with the pre-treatment of High Temperature High Pressure, be more conducive to the stripping of xylo-oligosaccharide in corn cob, improve the output that combinative enzyme hydrolysis prepares xylo-oligosaccharide; Ultrasonic assistant combinative enzyme hydrolysis corn cob is adopted to prepare xylo-oligosaccharide, have that extraction efficiency is high, hydrolysis time is short, the gentle advantage such as controlled and pollution-free of reaction conditions, this invention provides a kind of new production technique for preparing functional low polyxylose suitability for industrialized production with corn cob.
Embodiment
Embodiment 1: the preparation method of a kind of corn cob xylo-oligosaccharide of the present invention, prepare according to following steps:
(1) micronizing
Select drying, be starting material without mouldy corn cob, after being pulverized with supper micron mill, for subsequent use;
(2) High Temperature High Pressure pre-treatment
Add the buffered soln of pH 5.0 according to the amount of solid-liquid ratio 1:15, be then placed in high-pressure sterilizing pot, at 110 DEG C of temperature, High Temperature High Pressure pre-treatment 40min;
(3) ultrasonic assistant combinative enzyme hydrolysis
The prozyme be made up of according to 3:2 ratio zytase and cellulase of 0.8% is added in the pretreated corn cob feed liquid of High Temperature High Pressure, at ultrasonic power 250W, at ultrasonic temperature 50 DEG C, ultrasonic wave added enzymic hydrolysis 20min;
(4) go out enzyme
Corn cob solution after hydrolysis is incubated 15min at 80 DEG C, then centrifugation, collects supernatant liquor;
(5) decolour
With macroporous resin AB-8 according to 1.5% addition, under pH 6.0 and 40 DEG C of temperature, decolouring 2h;
(6) desalination
Use D301 and 001 × 7 anion-cation exchange resin according to the addition of 8% respectively, under pH 6.0 and 45 DEG C of temperature, desalination is carried out to xylo-oligosaccharide solution;
(7) alcohol precipitation
Xylo-oligosaccharide solution after desalination, after membrane separation concentration, adds the ethanol of 3 times of volumes, 4 DEG C of standing 12h, filtering solution, collecting precipitation;
(8) dry
Precipitation after filtering is put into vacuum drying oven, after drying, is xylo-oligosaccharide finished product.
Embodiment 2: the preparation method of a kind of corn cob xylo-oligosaccharide of the present invention, prepare according to following steps:
(1) micronizing
Select drying, be starting material without mouldy corn cob, after being pulverized with supper micron mill, for subsequent use;
(2) High Temperature High Pressure pre-treatment
Add the buffered soln of pH 6.0 according to the amount of solid-liquid ratio 1:30, be then placed in high-pressure sterilizing pot, at 120 DEG C of temperature, High Temperature High Pressure pre-treatment 30min;
(3) ultrasonic assistant combinative enzyme hydrolysis
The prozyme be made up of according to 3:2 ratio zytase and cellulase of 1.0% is added in the pretreated corn cob feed liquid of High Temperature High Pressure, at ultrasonic power 300W, at ultrasonic temperature 60 DEG C, ultrasonic wave added enzymic hydrolysis 10min;
(4) go out enzyme
Corn cob solution after hydrolysis is incubated 15min at 80 DEG C, then centrifugation, collects supernatant liquor;
(5) decolour
With macroporous resin AB-8 according to 2.0% addition, under pH 6.0 and 45 DEG C of temperature, decolouring 1.5h;
(6) desalination
Use D301 and 001 × 7 anion-cation exchange resin according to the addition of 10% respectively, under pH 6.0 and 50 DEG C of temperature, desalination is carried out to xylo-oligosaccharide solution;
(7) alcohol precipitation
Xylo-oligosaccharide solution after desalination, after membrane separation concentration, adds the ethanol of 4 times of volumes, 4 DEG C of standing 12h, filtering solution, collecting precipitation;
(8) dry
Precipitation after filtering is put into vacuum drying oven, after drying, is xylo-oligosaccharide finished product.
Claims (1)
1. a preparation method for corn cob xylo-oligosaccharide, is characterized in that comprising the following steps:
(1) micronizing
Select drying, be starting material without mouldy corn cob, after being pulverized with supper micron mill, for subsequent use;
(2) High Temperature High Pressure pre-treatment
Add the buffered soln of pH5.0 ~ 6.0 according to the amount of solid-liquid ratio 1:15 ~ 1:30, be then placed in high-pressure sterilizing pot, at 105 DEG C ~ 120 DEG C temperature, High Temperature High Pressure pre-treatment 30min ~ 50min;
(3) ultrasonic assistant combinative enzyme hydrolysis
The prozyme be made up of according to 3:2 ratio zytase and cellulase of 0.8% ~ 1.0% is added in the pretreated corn cob feed liquid of High Temperature High Pressure, at ultrasonic power 250W ~ 300W, at ultrasonic temperature 50 DEG C ~ 60 DEG C, ultrasonic wave added enzymic hydrolysis 10min ~ 20min;
(4) go out enzyme
Corn cob solution after hydrolysis is incubated 15min at 80 DEG C, then centrifugation, collects supernatant liquor;
(5) decolour
With macroporous resin AB-8 according to 1.5% ~ 2.5% addition, at pH6.0 and 40 DEG C ~ 45 DEG C temperature, decolouring 1.5h ~ 2h;
(6) desalination
Use D301 and 001 × 7 anion-cation exchange resin according to the addition of 8% ~ 10% respectively, at pH6.0 and 40 DEG C ~ 50 DEG C temperature, desalination is carried out to xylo-oligosaccharide solution;
(7) alcohol precipitation
Xylo-oligosaccharide solution after desalination, after membrane separation concentration, adds the ethanol of 2 ~ 4 times of volumes, 4 DEG C of standing 12h, filtering solution, collecting precipitation;
(8) dry
Precipitation after filtering is put into vacuum drying oven, after drying, is xylo-oligosaccharide finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510170829.0A CN104774887A (en) | 2015-04-13 | 2015-04-13 | Preparation method of corncob xylooligosaccharide |
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| CN201510170829.0A CN104774887A (en) | 2015-04-13 | 2015-04-13 | Preparation method of corncob xylooligosaccharide |
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| CN201510170829.0A Pending CN104774887A (en) | 2015-04-13 | 2015-04-13 | Preparation method of corncob xylooligosaccharide |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105440165A (en) * | 2015-10-20 | 2016-03-30 | 石河子大学 | Method for extracting xylan from cottonseed hulls by microwave-assisted alkali liquor |
| CN105603046A (en) * | 2016-01-22 | 2016-05-25 | 山东大学 | Method for analyzing and identifying functions of different kinds of xylanase in corn cob xylan enzymatic hydrolysate through fluorophore-assisted carbohydrate electrophoresis |
| CN106636480A (en) * | 2016-11-09 | 2017-05-10 | 河南坤之元生物科技有限公司 | Preparation method of xylooligosaccharide by corn |
| CN107557406A (en) * | 2017-09-26 | 2018-01-09 | 东北农业大学 | A kind of method that xylo-oligosaccharide is extracted from corncob |
| CN107868806A (en) * | 2017-11-27 | 2018-04-03 | 许昌学院 | A kind of method that xylo-oligosaccharide is prepared by lignocellulosic |
| CN110616237A (en) * | 2019-10-22 | 2019-12-27 | 天津科技大学 | Method for preparing xylo-oligosaccharide from steam-exploded plant fiber raw material |
| CN110628846A (en) * | 2019-07-04 | 2019-12-31 | 山东百龙创园生物科技股份有限公司 | Method for preparing xylooligosaccharide by high-temperature high-pressure treatment |
| WO2021000963A1 (en) * | 2019-07-04 | 2021-01-07 | 山东百龙创园生物科技股份有限公司 | Trichoderma reesei strain, culture method therefor, and application thereof |
| CN112301075A (en) * | 2020-11-03 | 2021-02-02 | 江苏康维生物有限公司 | Method for preparing feed-grade xylo-oligosaccharide from corncobs |
Citations (2)
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| CN102605021A (en) * | 2012-03-31 | 2012-07-25 | 天津现代职业技术学院 | Method for preparing xylo-oligosaccharide syrup and powdered sugar by using complex enzyme |
| CN103243138A (en) * | 2013-05-09 | 2013-08-14 | 许昌学院 | Method for preparing corncob xylooligosaccharide by a complex enzyme method |
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2015
- 2015-04-13 CN CN201510170829.0A patent/CN104774887A/en active Pending
Patent Citations (2)
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| CN102605021A (en) * | 2012-03-31 | 2012-07-25 | 天津现代职业技术学院 | Method for preparing xylo-oligosaccharide syrup and powdered sugar by using complex enzyme |
| CN103243138A (en) * | 2013-05-09 | 2013-08-14 | 许昌学院 | Method for preparing corncob xylooligosaccharide by a complex enzyme method |
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| 孙军涛等: "复合酶法制备玉米芯低聚木糖的研究", 《食品工业》 * |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105440165A (en) * | 2015-10-20 | 2016-03-30 | 石河子大学 | Method for extracting xylan from cottonseed hulls by microwave-assisted alkali liquor |
| CN105603046A (en) * | 2016-01-22 | 2016-05-25 | 山东大学 | Method for analyzing and identifying functions of different kinds of xylanase in corn cob xylan enzymatic hydrolysate through fluorophore-assisted carbohydrate electrophoresis |
| CN106636480A (en) * | 2016-11-09 | 2017-05-10 | 河南坤之元生物科技有限公司 | Preparation method of xylooligosaccharide by corn |
| CN107557406A (en) * | 2017-09-26 | 2018-01-09 | 东北农业大学 | A kind of method that xylo-oligosaccharide is extracted from corncob |
| CN107868806A (en) * | 2017-11-27 | 2018-04-03 | 许昌学院 | A kind of method that xylo-oligosaccharide is prepared by lignocellulosic |
| CN110628846A (en) * | 2019-07-04 | 2019-12-31 | 山东百龙创园生物科技股份有限公司 | Method for preparing xylooligosaccharide by high-temperature high-pressure treatment |
| CN110628846B (en) * | 2019-07-04 | 2020-07-03 | 山东百龙创园生物科技股份有限公司 | Method for preparing xylooligosaccharide by high-temperature high-pressure treatment |
| WO2021000963A1 (en) * | 2019-07-04 | 2021-01-07 | 山东百龙创园生物科技股份有限公司 | Trichoderma reesei strain, culture method therefor, and application thereof |
| CN112930391A (en) * | 2019-07-04 | 2021-06-08 | 山东百龙创园生物科技股份有限公司 | Trichoderma reesei and culture method and application thereof |
| CN110616237A (en) * | 2019-10-22 | 2019-12-27 | 天津科技大学 | Method for preparing xylo-oligosaccharide from steam-exploded plant fiber raw material |
| CN112301075A (en) * | 2020-11-03 | 2021-02-02 | 江苏康维生物有限公司 | Method for preparing feed-grade xylo-oligosaccharide from corncobs |
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Application publication date: 20150715 |