CN102605021A - Method for preparing xylo-oligosaccharide syrup and powdered sugar by using complex enzyme - Google Patents
Method for preparing xylo-oligosaccharide syrup and powdered sugar by using complex enzyme Download PDFInfo
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- CN102605021A CN102605021A CN2012100943726A CN201210094372A CN102605021A CN 102605021 A CN102605021 A CN 102605021A CN 2012100943726 A CN2012100943726 A CN 2012100943726A CN 201210094372 A CN201210094372 A CN 201210094372A CN 102605021 A CN102605021 A CN 102605021A
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Abstract
The invention relates to a method for preparing xylo-oligosaccharide syrup and powdered sugar by using a complex enzyme. The method comprises the following steps of: (1) crushing a cellulosic raw material and preparing the cellulosic raw material into a suspension; (2) adjusting the pH value of the suspension to be between 5.5 and 6.5, adding a high temperature-resistant alpha-amylase into the suspension, after raising the temperature to 85 to 95 DEG C, hydrolyzing the suspension, cooling the suspension, adjusting the pH value to be between 7.5 and 8.5, adding an alkali protease, stirring the suspension at a constant speed, after raising the temperature to 45 to 60 DEG C, hydrolyzing the suspension, and inactivating the enzyme; (3) centrifuging the suspension in which the enzyme is inactivated to precipitate, and washing and drying the deposit; (4) preparing the deposit into a dietary fiber suspension, adjusting the pH value to 5.6, and adding the liquid complex enzyme consisting of incision beta-1,4-xylanase, feruloyl esterase and fungal laccase into the suspension to obtain a xylo-oligosaccharide enzymatic hydrolysate; and (5) performing precise filtration, nano filtration, super filtration, desalination, decoloration and concentration on the xylo-oligosaccharide enzymatic hydrolysate to obtain the xylo-oligosaccharide syrup. The method is simple in process and wide in raw material source; the raw materials can be mixed; the production time is shortened; the production efficiency and the yield are improved.
Description
Technical field
The invention belongs to the xylooligosaccharides preparation field, especially a kind of method of utilizing prozyme to prepare xylooligosaccharides syrup, Icing Sugar.
Background technology
Xylooligosaccharides is claimed wood oligose again, be by 2-7 wood sugar molecule with β-1,4 glycosidic link be combined into, be the mixture of staple with xylo-bioses and xylotriose.Xylooligosaccharides is a kind of important function property food; Except having low-heat, good physicochemical characteristics such as stable, safe, nontoxic; Also have the unique physiological property that promotes beneficial flora breeding in the enteron aisle, suppresses the pernicious bacteria growth, can be widely used in fields such as food, beverage, health care.
The production of xylooligosaccharides at present mainly contains acid degradation and two kinds of methods of biological degradation.When adopting the acid degradation method, the composition of xylan degrading speed and product is difficult to control, is difficult to the preparation high-purity oligoxylose.Biological degradation method comprises microbe fermentation method and enzyme hydrolysis method.The very difficulty because the product of microbe fermentation method is purified, therefore the suitability for industrialized production xylooligosaccharides mainly adopts enzyme hydrolysis method at present, and existing enzyme hydrolysis method is mainly used single enzyme and is hydrolyzed; And corresponding raw material also is a single kind, such as single corn cob, bagasse, straw, Testa Tritici, cotton seed hulls or vegetable seed shell, and raw material type uses and is restricted during preparation; Moreover enzymolysis time is longer; Generally need 16-24 hour, the xylooligosaccharides productive rate is lower, generally has only 15-20%.
Summary of the invention
The objective of the invention is to overcome the deficiency of prior art, provide a kind of prozyme that utilizes to prepare the syrupy method of xylooligosaccharides, this method technology is simple, and raw material sources extensively also can mix use, have shortened the PT, have improved production efficiency and productive rate.
Another object of the present invention is to provide a kind of method of utilizing prozyme to prepare the xylooligosaccharides Icing Sugar.
The present invention solves its technical problem and realizes through following technical scheme:
A kind of prozyme that utilizes prepares the syrupy method of xylooligosaccharides, and the step of its method is:
(1), cellulosic material is pulverized, pulverizing the back crosses to add after the 40-60 mesh sieve cleaning removal of impurities and is configured to cellulosic material suspension-s in the entry;
(2), adopt Sodium phosphate, dibasic-citric acid solution to regulate cellulosic material pH of suspension value to 5.5-6.5; Add the high temperature resistant AMS of cellulosic material suspension-s weight 0.5-1.5%, at the uniform velocity be warming up to 85-95 ℃ of posthydrolysis 30-45min under the agitation condition at stirrer; Be cooled to 40-45 ℃; Adopt Sodium phosphate, dibasic-citric acid solution to regulate cellulosic material pH of suspension value to 7.5-8.5; Add the Sumizyme MP of cellulosic material suspension-s weight 2.5-3.5%, at the uniform velocity be warming up to 45-60 ℃ of posthydrolysis 100-150min under the agitation condition at stirrer; After reaction finishes, under 100 ℃, boil the 10-15min enzyme that goes out, enzyme suspension-s obtains going out;
(3), the enzyme suspension-s centrifugation of will going out, abandoning supernatant is washed 3-5 time with zero(ppm) water, obtain throw out and after drying treatment, obtain dietary fibre materials, with dietary fibre materials at 30-40 ℃ of following dry 24-30h, to its moisture content be 10-12%;
(4), dried dietary fibre materials is mixed with 60g/L food fibre suspension-s; Adopt Sodium phosphate, dibasic-citric acid solution to regulate food fibre pH of suspension value to 5.6; Add inscribe β-1, the prozyme liquid that 4-zytase, feruloyl esterase and fungal laccase constitute reacts 6-12h down at 55 ℃; Reaction finishes after-filtration, gets the xylooligosaccharides enzymolysis solution;
(5), the xylooligosaccharides enzymolysis solution is held back the nanofiltration solution that relative molecular mass is 100-800 through smart filter back through nf membrane; Hold back the ultrafiltration solution that relative molecular mass is 1500-20000 through ultra-filtration membrane again, obtain xylooligosaccharides syrup finished product with the desalination of ultrafiltration solution, decolouring, after concentrating.
And described cellulosic material is corn cob, bagasse, straw, Testa Tritici, cotton seed hulls and vegetable seed shell.
And the weight ratio of described cellulosic material and water is 1: 5-1: 15.
And; Described prozyme liquid is for adding the inscribe β-1 of food fibre suspension-s weight 0.6%-0.7%, the mixed solution of 4-zytase, food fibre suspension-s weight 0.2%-0.4% feruloyl esterase and food fibre suspension-s weight 0.15%-0.25% fungal laccase.
A kind of method of utilizing prozyme to prepare the xylooligosaccharides Icing Sugar, it will utilize prozyme to prepare the xylooligosaccharides syrup process spraying drying that the syrupy method of xylooligosaccharides obtains, and promptly obtain xylooligosaccharides Icing Sugar finished product.
Advantage of the present invention and beneficial effect are:
1, this utilizes prozyme to prepare the method for xylooligosaccharides syrup, Icing Sugar; Can be applied to different cellulosic materials such as corn cob, bagasse and straw simultaneously, enzymolysis time is short, and the traditional technology enzymolysis time is 16-24h; This technology enzymolysis time is 6-12h; The high traditional technology enzymolysis of xylooligosaccharides enzymolysis productive rate productive rate is generally about 15-20%, and this technology enzymolysis productive rate can reach about 25-30%, has the favorable industrial application prospect.
2, technology of the present invention is simple, and raw material sources extensively also can mix use, have shortened the PT, have improved production efficiency and productive rate, are that a kind of safe and reliable prozyme that utilizes prepares the method for xylooligosaccharides syrup, Icing Sugar.
Embodiment
Through specific embodiment the present invention is made further detailed description below, following examples are descriptive, are not determinate, can not limit protection scope of the present invention with this.
Corn cob, bagasse, straw, Testa Tritici, cotton seed hulls and vegetable seed shell among the present invention; Sodium phosphate, dibasic-citric acid solution, high temperature resistant AMS, Sumizyme MP, inscribe β-1,4-zytase, feruloyl esterase and fungal laccase all can buy on market and obtain.
Embodiment 1
A kind of prozyme that utilizes prepares the syrupy method of xylooligosaccharides, and the step of its method is:
(1), cellulosic material is pulverized, the cellulosic material that present embodiment adopts is the mixture of corn cob, bagasse, straw, pulverizes the back and crosses the water that adds 5 times of weight of mixture after the 40 mesh sieves cleaning removal of impurities, is configured to cellulosic material suspension-s; Cellulosic material also can be Testa Tritici, cotton seed hulls and vegetable seed shell;
(2), adopt Sodium phosphate, dibasic-citric acid solution to regulate cellulosic material pH of suspension value to 5.5, add the high temperature resistant AMS of cellulosic material suspension-s weight 0.5%, at the uniform velocity be warming up to 85 ℃ of posthydrolysis 30min under the agitation condition at stirrer; Be cooled to 40 ℃, adopt Sodium phosphate, dibasic-citric acid solution to regulate cellulosic material pH of suspension value to 7.5, add the Sumizyme MP of cellulosic material suspension-s weight 2.5%, at the uniform velocity be warming up to 45 ℃ of posthydrolysis 100min under the agitation condition at stirrer; After reaction finishes, under 100 ℃, boil the 10min enzyme that goes out, enzyme suspension-s obtains going out;
(3), the enzyme suspension-s centrifugation of will going out, abandoning supernatant is washed 3 times with zero(ppm) water, obtain throw out and after drying treatment, obtain dietary fibre materials, with dietary fibre materials at 30 ℃ of following dry 24h, to its moisture content be 10%;
(4), dried dietary fibre materials is mixed with 60g/L food fibre suspension-s; Adopt Sodium phosphate, dibasic-citric acid solution to regulate food fibre pH of suspension value to 5.6; The inscribe β-1 that adds food fibre suspension-s weight 0.6%, the prozyme liquid that 4-zytase, food fibre suspension-s weight 0.2% feruloyl esterase and food fibre suspension-s weight 0.15% fungal laccase constitute reacts 6h down at 55 ℃; Reaction finishes after-filtration, gets the xylooligosaccharides enzymolysis solution;
(5), the xylooligosaccharides enzymolysis solution being held back relative molecular mass through smart filter back through nf membrane is 100 nanofiltration solution; Holding back relative molecular mass through ultra-filtration membrane again is 1500 ultrafiltration solution, obtains xylooligosaccharides syrup finished product with the desalination of ultrafiltration solution, decolouring, after concentrating.
A kind of method of utilizing prozyme to prepare the xylooligosaccharides Icing Sugar, it utilizes prozyme to prepare the xylooligosaccharides syrup process spraying drying that the syrupy method of xylooligosaccharides obtains, and promptly obtains xylooligosaccharides Icing Sugar finished product.
Embodiment 2
A kind of prozyme that utilizes prepares the syrupy method of xylooligosaccharides, and the step of its method is:
(1), cellulosic material is pulverized, this cellulosic material is the mixture of Testa Tritici, cotton seed hulls and vegetable seed shell, pulverizes the back and crosses the water that adds 15 times of mixture weight after the 60 mesh sieves cleaning removal of impurities, is configured to cellulosic material suspension-s;
(2), adopt Sodium phosphate, dibasic-citric acid solution to regulate cellulosic material pH of suspension value to 6.5, add the high temperature resistant AMS of cellulosic material suspension-s weight 1.5%, at the uniform velocity be warming up to 95 ℃ of posthydrolysis 45min under the agitation condition at stirrer; Be cooled to 45 ℃, adopt Sodium phosphate, dibasic-citric acid solution to regulate cellulosic material pH of suspension value to 8.5, add the Sumizyme MP of cellulosic material suspension-s weight 3.5%, at the uniform velocity be warming up to 60 ℃ of posthydrolysis 150min under the agitation condition at stirrer; After reaction finishes, under 100 ℃, boil the 15min enzyme that goes out, enzyme suspension-s obtains going out;
(3), the enzyme suspension-s centrifugation of will going out, abandoning supernatant is washed 5 times with zero(ppm) water, obtain throw out and after drying treatment, obtain dietary fibre materials, with dietary fibre materials at 40 ℃ of following dry 30h, to its moisture content be 12%;
(4), dried dietary fibre materials is mixed with 60g/L food fibre suspension-s; Adopt Sodium phosphate, dibasic-citric acid solution to regulate food fibre pH of suspension value to 5.6; The inscribe β-1 that adds food fibre suspension-s weight 0.7%, the prozyme liquid that 4-zytase, food fibre suspension-s weight 0.4% feruloyl esterase and food fibre suspension-s weight 0.25% fungal laccase constitute reacts 12h down at 55 ℃; Reaction finishes after-filtration, gets the xylooligosaccharides enzymolysis solution;
(5), the xylooligosaccharides enzymolysis solution being held back relative molecular mass through smart filter back through nf membrane is 800 nanofiltration solution; Holding back relative molecular mass through ultra-filtration membrane again is 20000 ultrafiltration solution, obtains xylooligosaccharides syrup finished product with the desalination of ultrafiltration solution, decolouring, after concentrating.
A kind of method of utilizing prozyme to prepare the xylooligosaccharides Icing Sugar, it utilizes prozyme to prepare the xylooligosaccharides syrup process spraying drying that the syrupy method of xylooligosaccharides obtains, and promptly obtains xylooligosaccharides Icing Sugar finished product.
Embodiment 3
A kind of prozyme that utilizes prepares the syrupy method of xylooligosaccharides, and the step of its method is:
(1), cellulosic material is pulverized, the present embodiment cellulosic material is a corn cob, pulverizes the back and crosses the water that adds 7 times of corn cob weight after the 50 mesh sieves cleaning removal of impurities, is configured to cellulosic material suspension-s;
(2), adopt Sodium phosphate, dibasic-citric acid solution to regulate cellulosic material pH of suspension value to 6, add the high temperature resistant AMS of cellulosic material suspension-s weight 1%, at the uniform velocity be warming up to 90 ℃ of posthydrolysis 38min under the agitation condition at stirrer; Be cooled to 43 ℃, adopt Sodium phosphate, dibasic-citric acid solution to regulate cellulosic material pH of suspension value to 8, add the Sumizyme MP of cellulosic material suspension-s weight 3%, at the uniform velocity be warming up to 53 ℃ of posthydrolysis 125min under the agitation condition at stirrer; After reaction finishes, under 100 ℃, boil the 13min enzyme that goes out, enzyme suspension-s obtains going out;
(3), the enzyme suspension-s centrifugation of will going out, abandoning supernatant is washed 4 times with zero(ppm) water, obtain throw out and after drying treatment, obtain dietary fibre materials, with dietary fibre materials at 35 ℃ of following dry 27h, to its moisture content be 11%;
(4), dried dietary fibre materials is mixed with 60g/L food fibre suspension-s; Adopt Sodium phosphate, dibasic-citric acid solution to regulate food fibre pH of suspension value to 5.6; The inscribe β-1 that adds food fibre suspension-s weight 0.65%, the prozyme liquid that 4-zytase, food fibre suspension-s weight 0.3% feruloyl esterase and food fibre suspension-s weight 0.2% fungal laccase constitute reacts 9h down at 55 ℃; Reaction finishes after-filtration, gets the xylooligosaccharides enzymolysis solution;
(5), the xylooligosaccharides enzymolysis solution being held back relative molecular mass through smart filter back through nf membrane is 450 nanofiltration solution; Holding back relative molecular mass through ultra-filtration membrane again is 11000 ultrafiltration solution, obtains xylooligosaccharides syrup finished product with the desalination of ultrafiltration solution, decolouring, after concentrating.
A kind of method of utilizing prozyme to prepare the xylooligosaccharides Icing Sugar, it utilizes prozyme to prepare the xylooligosaccharides syrup process spraying drying that the syrupy method of xylooligosaccharides obtains, and promptly obtains xylooligosaccharides Icing Sugar finished product.
Embodiment 4
Use corn cob, straw and Testa Tritici composition mixing raw material that the present invention gets equivalent, add water and stir, cross colloidal mill.Regulate amount of water, the formulation liquor ratio is 1: 12 a mixing raw material suspension-s, and regulating pH is 5.6, and controlled temperature adds 1.5% fire resistant alpha-diastase between 95～100 ℃, constantly stirs hydrolysis 30min down.Suspension temperature drops to 50 ℃, and regulating pH is 9.0, adds 3% Sumizyme MP, enzymolysis 120min.Centrifugal 20min under the 5000rmin-1, abandoning supernatant is washed throw out 3 times with hot distilled water, and throw out is placed in the baking oven, dries by the fire 10h down at 60 ℃, obtains removing starch and proteic food fibre.Get the food fibre after drying is weighed, be mixed with 60g.L
-1Suspension-s, regulating pH is 5.6, adds the prozyme that feruloyl esterase, zytase and laccase are formed; Wherein the ferulic acid ester enzyme dosage 0.20%, zytase consumption 0.64%, laccase consumption 0.16%; Reaction 6h under 55 ℃, reaction finishes after-filtration, gets the xylooligosaccharides enzymolysis solution; Record xylan enzymolysis productive rate under this condition and reach 27.2%.
Claims (5)
1. one kind is utilized prozyme to prepare the syrupy method of xylooligosaccharides, it is characterized in that: steps of the method are:
(1), cellulosic material is pulverized, pulverizing the back crosses to add after the 40-60 mesh sieve cleaning removal of impurities and is configured to cellulosic material suspension-s in the entry;
(2), adopt Sodium phosphate, dibasic-citric acid solution to regulate cellulosic material pH of suspension value to 5.5-6.5; Add the high temperature resistant AMS of cellulosic material suspension-s weight 0.5-1.5%, at the uniform velocity be warming up to 85-95 ℃ of posthydrolysis 30-45min under the agitation condition at stirrer; Be cooled to 40-45 ℃; Adopt Sodium phosphate, dibasic-citric acid solution to regulate cellulosic material pH of suspension value to 7.5-8.5; Add the Sumizyme MP of cellulosic material suspension-s weight 2.5-3.5%, at the uniform velocity be warming up to 45-60 ℃ of posthydrolysis 100-150min under the agitation condition at stirrer; After reaction finishes, under 100 ℃, boil the 10-15min enzyme that goes out, enzyme suspension-s obtains going out;
(3), the enzyme suspension-s centrifugation of will going out, abandoning supernatant is washed 3-5 time with zero(ppm) water, obtain throw out and after drying treatment, obtain dietary fibre materials, with dietary fibre materials at 30-40 ℃ of following dry 24-30h, to its moisture content be 10-12%;
(4), dried dietary fibre materials is mixed with 60g/L food fibre suspension-s; Adopt Sodium phosphate, dibasic-citric acid solution to regulate food fibre pH of suspension value to 5.6; Add inscribe β-1, the prozyme liquid that 4-zytase, feruloyl esterase and fungal laccase constitute reacts 6-12h down at 55 ℃; Reaction finishes after-filtration, gets the xylooligosaccharides enzymolysis solution;
(5), the xylooligosaccharides enzymolysis solution is held back the nanofiltration solution that relative molecular mass is 100-800 through smart filter back through nf membrane; Hold back the ultrafiltration solution that relative molecular mass is 1500-20000 through ultra-filtration membrane again, obtain xylooligosaccharides syrup finished product with the desalination of ultrafiltration solution, decolouring, after concentrating.
2. the prozyme that utilizes according to claim 1 prepares the syrupy method of xylooligosaccharides, it is characterized in that: described cellulosic material is corn cob, bagasse, straw, Testa Tritici, cotton seed hulls and vegetable seed shell.
3. the prozyme that utilizes according to claim 1 prepares the syrupy method of xylooligosaccharides, and it is characterized in that: the weight ratio of described cellulosic material and water is 1: 5-1: 15.
4. the prozyme that utilizes according to claim 1 prepares the syrupy method of xylooligosaccharides; It is characterized in that: described prozyme liquid is for adding the inscribe β-1 of food fibre suspension-s weight 0.6%-0.7%, the mixed solution of 4-zytase, food fibre suspension-s weight 0.2%-0.4% feruloyl esterase and food fibre suspension-s weight 0.15%-0.25% fungal laccase.
5. method of utilizing prozyme to prepare the xylooligosaccharides Icing Sugar; It is characterized in that: will prepare the xylooligosaccharides syrup process spraying drying that the syrupy method of xylooligosaccharides obtains like claim 1 or 2 or the 3 or 4 described prozymes that utilize, and promptly obtain xylooligosaccharides Icing Sugar finished product.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104357512A (en) * | 2014-11-07 | 2015-02-18 | 宜宾雅泰生物科技有限公司 | Membrane concentration process for producing food-grade xylo-oligosaccharide from viscose fiber squeezed liquor |
| CN104357590A (en) * | 2014-11-07 | 2015-02-18 | 宜宾雅泰生物科技有限公司 | Membrane concentration process for preparing food-grade xylo-oligosaccharide from squeezed liquor for viscose fiber production |
| CN104774887A (en) * | 2015-04-13 | 2015-07-15 | 许昌学院 | Preparation method of corncob xylooligosaccharide |
| CN105779530A (en) * | 2016-05-09 | 2016-07-20 | 内蒙古科技大学 | Waste-fermented-grain based microcrystalline cellulose and preparation method thereof |
| CN106173740A (en) * | 2016-07-19 | 2016-12-07 | 唐春艳 | A kind of Testa Tritici water soluble dietary fiber beverage and preparation method thereof |
| CN106923310A (en) * | 2017-04-12 | 2017-07-07 | 盐城宇峰食品科技有限公司 | A kind of syrup dedicated production technology of drink |
| CN110042133A (en) * | 2019-04-22 | 2019-07-23 | 齐鲁工业大学 | A kind of method that biological enzyme collaboration physical-chemical process purifying prepares poplar xylo-oligosaccharide |
| CN110973421A (en) * | 2019-11-29 | 2020-04-10 | 中国农业科学院农产品加工研究所 | High-content xylo-oligosaccharide corn bamboo shoot beverage and preparation method thereof |
| CN111109445A (en) * | 2019-12-16 | 2020-05-08 | 厦门大学 | Comprehensive processing method of bamboo shoot processing residue fine particles |
| CN108354061B (en) * | 2018-02-28 | 2021-07-16 | 苏州昆蓝生物科技有限公司 | Production method of intestinal regulator |
| CN114350723A (en) * | 2021-12-27 | 2022-04-15 | 海南拉风农业科技有限公司 | Method for preparing xylooligosaccharide by using black corncobs |
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| CN104357590A (en) * | 2014-11-07 | 2015-02-18 | 宜宾雅泰生物科技有限公司 | Membrane concentration process for preparing food-grade xylo-oligosaccharide from squeezed liquor for viscose fiber production |
| CN104357512A (en) * | 2014-11-07 | 2015-02-18 | 宜宾雅泰生物科技有限公司 | Membrane concentration process for producing food-grade xylo-oligosaccharide from viscose fiber squeezed liquor |
| CN104774887A (en) * | 2015-04-13 | 2015-07-15 | 许昌学院 | Preparation method of corncob xylooligosaccharide |
| CN105779530A (en) * | 2016-05-09 | 2016-07-20 | 内蒙古科技大学 | Waste-fermented-grain based microcrystalline cellulose and preparation method thereof |
| CN105779530B (en) * | 2016-05-09 | 2019-11-08 | 内蒙古科技大学 | A kind of microcrystalline cellulose and preparation method thereof based on spirit distiller grain |
| CN106173740A (en) * | 2016-07-19 | 2016-12-07 | 唐春艳 | A kind of Testa Tritici water soluble dietary fiber beverage and preparation method thereof |
| CN106923310A (en) * | 2017-04-12 | 2017-07-07 | 盐城宇峰食品科技有限公司 | A kind of syrup dedicated production technology of drink |
| CN108354061B (en) * | 2018-02-28 | 2021-07-16 | 苏州昆蓝生物科技有限公司 | Production method of intestinal regulator |
| CN110042133A (en) * | 2019-04-22 | 2019-07-23 | 齐鲁工业大学 | A kind of method that biological enzyme collaboration physical-chemical process purifying prepares poplar xylo-oligosaccharide |
| CN110042133B (en) * | 2019-04-22 | 2021-06-04 | 齐鲁工业大学 | Method for purifying and preparing poplar xylo-oligosaccharide by biological enzyme synergistic physical and chemical method |
| CN110973421A (en) * | 2019-11-29 | 2020-04-10 | 中国农业科学院农产品加工研究所 | High-content xylo-oligosaccharide corn bamboo shoot beverage and preparation method thereof |
| CN111109445A (en) * | 2019-12-16 | 2020-05-08 | 厦门大学 | Comprehensive processing method of bamboo shoot processing residue fine particles |
| CN114350723A (en) * | 2021-12-27 | 2022-04-15 | 海南拉风农业科技有限公司 | Method for preparing xylooligosaccharide by using black corncobs |
| CN114350723B (en) * | 2021-12-27 | 2024-03-26 | 海南拉风农业科技有限公司 | Method for preparing xylo-oligosaccharide by using black corncob |
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Application publication date: 20120725 |