CN112301075A - Method for preparing feed-grade xylo-oligosaccharide from corncobs - Google Patents
Method for preparing feed-grade xylo-oligosaccharide from corncobs Download PDFInfo
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- CN112301075A CN112301075A CN202011210287.2A CN202011210287A CN112301075A CN 112301075 A CN112301075 A CN 112301075A CN 202011210287 A CN202011210287 A CN 202011210287A CN 112301075 A CN112301075 A CN 112301075A
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C08B37/0057—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Xylans, i.e. xylosaccharide, e.g. arabinoxylan, arabinofuronan, pentosans; (beta-1,3)(beta-1,4)-D-Xylans, e.g. rhodymenans; Hemicellulose; Derivatives thereof
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Abstract
The invention discloses a method for preparing feed-grade xylo-oligosaccharide by using corncobs, which relates to the technical field of preparation of xylo-oligosaccharide and comprises the following steps: (1) pretreating the corncobs; (2) pulping the corncobs; (3) carrying out enzymolysis treatment; (4) separating and purifying; (5) concentrating and purifying; (6) and (5) crystallizing to prepare. This method for preparing fodder level xylo-oligosaccharide with corncob, the washing to the corncob, the drying, crushing and grinding treatment are convenient for obtain the corncob powder, be convenient for obtain the thick liquid through corncob slurrying process, the compound enzyme that adopts xylanase and cellulase mixture to make is convenient for carry out abundant enzymolysis to the thick liquid, it carries out the superfine grinding to assist the ultrasonic wave at the enzymolysis in-process, can fully smash and degrade fibre and lignin in the rice core powder, so that generate xylo-oligosaccharide through hydrolysis, through the process of separation purification, concentration purification and crystallization preparation, be favorable to filtering impurity and obtain the higher xylo-oligosaccharide of purity.
Description
Technical Field
The invention relates to the technical field of xylo-oligosaccharide preparation, in particular to a method for preparing feed-grade xylo-oligosaccharide by using corncobs.
Background
The xylo-oligosaccharide is also called xylo-oligosaccharide, and is a functional polysaccharide formed by combining 2-7 xylose molecules by beta-1, 4 glycosidic bonds. The bifidus factor function is 10-20 times of other polymeric saccharides. Compared with the soybean oligosaccharide, fructo-oligosaccharide, isomaltose oligosaccharide and the like which are commonly used by people, the xylo-oligosaccharide has unique advantages and can selectively promote the proliferation activity of intestinal bifidobacteria.
The existing method for preparing feed-grade xylo-oligosaccharide by using corncobs is complex in process, low in extraction efficiency of the xylo-oligosaccharide, inconvenient for degradation of fibers and lignin inside the corncobs, and possibly has the condition of insufficient purity of the obtained xylo-oligosaccharide, so that a method for preparing the feed-grade xylo-oligosaccharide by using the corncobs is provided.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for preparing feed-grade xylooligosaccharide by using corncobs, which solves the problems that the existing method for preparing feed-grade xylooligosaccharide by using corncobs in the background art is complex in process, low in extraction efficiency of xylooligosaccharide, inconvenient for degradation of fibers and lignin in the corncobs and possibly has the condition of insufficient purity of the obtained xylooligosaccharide.
In order to achieve the purpose, the invention is realized by the following technical scheme, and the method for preparing the feed-grade xylo-oligosaccharide by using the corncobs comprises the following steps:
(1) pretreating the corncobs;
(2) pulping the corncobs;
(3) carrying out enzymolysis treatment;
(4) separating and purifying;
(5) concentrating and purifying;
(6) and (5) crystallizing to prepare.
Optionally, the method for preparing feed-grade xylooligosaccharide from corncobs comprises the following specific steps:
(1) pretreatment of corncob
Selecting corncobs, cleaning, drying, crushing and grinding the corncobs, wherein the corncobs can be ground by adopting coarse grinding equipment and fine grinding equipment during grinding, and then corncob powder is obtained;
(2) corncob pulping
Adding the ground corncob powder into a reaction kettle, adding distilled water, fully stirring and mixing the corncob powder and the distilled water by adopting stirring equipment, and cooking the slurry of the corncob powder to form slurry;
(3) and performing enzymolysis treatment
Adding a complex enzyme into the slurry for enzymolysis, continuously stirring at a constant speed during the enzymolysis, assisting with ultrasonic wave for superfine grinding to promote the enzymolysis, taking water as a reaction solvent to dissolve out hemicellulose in the corncobs and perform hydrolysis reaction to generate xylo-oligosaccharide, cooling the xylo-oligosaccharide after the enzymolysis reaction, and then obtaining a hydrolysis mixed solution;
(4) and separating and purifying
Performing solid-liquid classification on the hydrolysis mixed solution to obtain a filtrate which is the xylo-oligosaccharide hydrolysis sugar solution, and then filtering the part of the xylo-oligosaccharide hydrolysis sugar solution by adopting an ultrafiltration membrane to obtain the filtrate of the xylo-oligosaccharide hydrolysis sugar solution;
(5) concentrating and purifying
Concentrating the filtrate of the xylo-oligosaccharide hydrolyzed sugar solution by using an evaporator, and purifying to obtain high-purity xylo-oligosaccharide syrup liquid after concentration;
(6) and crystal preparation
And finally, carrying out vacuum drying on the high-purity xylo-oligosaccharide syrup liquid concentrated by the vacuum dryer to obtain crystalline xylose, thus obtaining the finished feed-grade xylo-oligosaccharide.
Optionally, in the corn cob pulping process in the step (2), the ratio of the corn cob powder to the distilled water is 1: 10.
Optionally, in the corn cob pulping process in the step (2), the stirring speed of the stirring equipment is 60-80 r/m.
Optionally, in the corn cob pulping process in the step (2), the cooking temperature of the corn cob powder slurry is 90-100 ℃, and the cooking time is 1-2 h.
Optionally, the complex enzyme in the enzymolysis treatment in the step (3) is prepared by mixing xylanase and cellulase, and the ratio of the xylanase to the cellulase is 2: 1.
Optionally, the enzymolysis temperature of the slurry in the enzymolysis treatment process in the step (3) is 50-60 ℃, and the enzymolysis time is 1-1.5 h.
The invention provides a method for preparing feed-grade xylo-oligosaccharide by using corncobs, which has the following beneficial effects: the method for preparing feed-grade xylo-oligosaccharide from corncobs is convenient for obtaining corncob powder through cleaning, drying, crushing and grinding the corncobs, the corn cob pulping process is convenient for obtaining the pulp, the complex enzyme prepared by mixing the xylanase and the cellulase is convenient for carrying out full enzymolysis on the pulp, in the enzymolysis process, ultrasonic waves are used for carrying out superfine grinding, so that the fiber and the lignin in the rice core powder can be fully ground and degraded, thereby facilitating the generation of xylo-oligosaccharide through hydrolysis reaction, through the processes of separation, purification, concentration and purification and crystallization preparation, the method is beneficial to filtering impurities to obtain high-purity xylo-oligosaccharide, the prepared xylo-oligosaccharide is rich in active ingredients such as polysaccharide, active probiotics, cellulase and the like, promotes the utilization of animals to feed, can be used as feed-grade xylo-oligosaccharide, and is convenient to use in the field of livestock and poultry feed.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to specific embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
A method for preparing feed-grade xylo-oligosaccharide from corncobs comprises the following steps:
(1) pretreating the corncobs;
(2) pulping the corncobs;
(3) carrying out enzymolysis treatment;
(4) separating and purifying;
(5) concentrating and purifying;
(6) and (5) crystallizing to prepare.
A method for preparing feed-grade xylo-oligosaccharide by using corncobs comprises the following specific steps:
(1) pretreatment of corncob
Selecting corncobs, cleaning, drying, crushing and grinding the corncobs, wherein the corncobs can be ground by adopting coarse grinding equipment and fine grinding equipment during grinding, and then corncob powder is obtained;
(2) corncob pulping
Adding the ground corncob powder into a reaction kettle, adding distilled water, fully stirring and mixing the corncob powder and the distilled water by adopting stirring equipment, and cooking the slurry of the corncob powder to form slurry;
in the corn cob pulping process, the ratio of the corn cob powder to the distilled water is 1: 10, so that the pulp is convenient to prepare, in the corn cob pulping process, the stirring speed of the stirring equipment is 60-80r/m, the corn cob powder can be fully stirred, in the corn cob pulping process, the pulp cooking temperature of the corn cob powder is 90-100 ℃, the cooking time is 1-2 hours, and the subsequent hydrolysis of the corn cob powder pulp is convenient;
(3) and performing enzymolysis treatment
Adding a complex enzyme into the slurry for enzymolysis, continuously stirring at a constant speed during the enzymolysis, assisting with ultrasonic wave for superfine grinding to promote the enzymolysis, taking water as a reaction solvent to dissolve out hemicellulose in the corncobs and perform hydrolysis reaction to generate xylo-oligosaccharide, cooling the xylo-oligosaccharide after the enzymolysis reaction, and then obtaining a hydrolysis mixed solution;
the complex enzyme in the enzymolysis treatment process is prepared by mixing xylanase and cellulase, the ratio of the xylanase to the cellulase is 2: 1, the complex enzyme is convenient for carrying out full enzymolysis on the slurry, the enzymolysis temperature of the slurry in the enzymolysis treatment process is 50-60 ℃, the enzymolysis time is 1-1.5h, and the enzymolysis is facilitated;
(4) and separating and purifying
Performing solid-liquid classification on the hydrolysis mixed solution to obtain a filtrate which is the xylo-oligosaccharide hydrolysis sugar solution, and then filtering the part of the xylo-oligosaccharide hydrolysis sugar solution by adopting an ultrafiltration membrane to obtain the filtrate of the xylo-oligosaccharide hydrolysis sugar solution;
(5) concentrating and purifying
Concentrating the filtrate of the xylo-oligosaccharide hydrolyzed sugar solution by using an evaporator, and purifying to obtain high-purity xylo-oligosaccharide syrup liquid after concentration;
(6) and crystal preparation
And finally, carrying out vacuum drying on the high-purity xylo-oligosaccharide syrup liquid concentrated by the vacuum dryer to obtain crystalline xylose, thus obtaining the finished feed-grade xylo-oligosaccharide.
In summary, the method for preparing the feed-grade xylo-oligosaccharide by using the corncob comprises the following specific steps:
(1) pretreatment of the corncobs: selecting corncobs, cleaning, drying, crushing and grinding the corncobs, wherein the corncobs can be ground by adopting coarse grinding equipment and fine grinding equipment during grinding, and then corncob powder is obtained;
(2) corncob pulping: adding the ground corncob powder into a reaction kettle, adding distilled water, wherein the ratio of the corncob powder to the distilled water is 1: 10, fully stirring and mixing the corncob powder and the distilled water by adopting stirring equipment, wherein the stirring speed of the stirring equipment is 60-80r/m, and simultaneously cooking the serous fluid of the corncob powder, the cooking temperature of the serous fluid of the corncob powder is 90-100 ℃, and the cooking time is 1-2h, so that serous fluid is formed;
(3) and (3) enzymolysis treatment: then adding a complex enzyme into the slurry for enzymolysis, wherein the complex enzyme is prepared by mixing xylanase and cellulase at a ratio of 2: 1, stirring at a constant speed continuously during the enzymolysis, carrying out superfine grinding by using ultrasonic waves to promote the enzymolysis, taking water as a reaction solvent to dissolve out hemicellulose in the corncobs and carrying out hydrolysis reaction to generate xylo-oligosaccharide, the enzymolysis temperature of the slurry is 50-60 ℃, the enzymolysis time is 1-1.5h, cooling the slurry after the enzymolysis reaction, and then obtaining a hydrolysis mixed solution;
(4) and separation and purification: performing solid-liquid classification on the hydrolysis mixed solution to obtain a filtrate which is the xylo-oligosaccharide hydrolysis sugar solution, and then filtering the part of the xylo-oligosaccharide hydrolysis sugar solution by adopting an ultrafiltration membrane to obtain the filtrate of the xylo-oligosaccharide hydrolysis sugar solution;
(5) and concentrating and purifying: concentrating the filtrate of the xylo-oligosaccharide hydrolyzed sugar solution by using an evaporator, and purifying to obtain high-purity xylo-oligosaccharide syrup liquid after concentration;
(6) and (3) crystal preparation: and (3) carrying out vacuum drying on the high-purity xylo-oligosaccharide syrup liquid concentrated by the vacuum dryer to obtain crystalline xylose, thus obtaining the finished feed-grade xylo-oligosaccharide.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (7)
1. A method for preparing feed-grade xylo-oligosaccharide by using corncobs is characterized by comprising the following steps:
(1) pretreating the corncobs;
(2) pulping the corncobs;
(3) carrying out enzymolysis treatment;
(4) separating and purifying;
(5) concentrating and purifying;
(6) and (5) crystallizing to prepare.
2. The method for preparing feed-grade xylo-oligosaccharide from corncobs as claimed in claim 1, wherein the method for preparing feed-grade xylo-oligosaccharide from corncobs comprises the following specific steps:
(1) pretreatment of corncob
Selecting corncobs, cleaning, drying, crushing and grinding the corncobs, wherein the corncobs can be ground by adopting coarse grinding equipment and fine grinding equipment during grinding, and then corncob powder is obtained;
(2) corncob pulping
Adding the ground corncob powder into a reaction kettle, adding distilled water, fully stirring and mixing the corncob powder and the distilled water by adopting stirring equipment, and cooking the slurry of the corncob powder to form slurry;
(3) and performing enzymolysis treatment
Adding a complex enzyme into the slurry for enzymolysis, continuously stirring at a constant speed during the enzymolysis, assisting with ultrasonic wave for superfine grinding to promote the enzymolysis, taking water as a reaction solvent to dissolve out hemicellulose in the corncobs and perform hydrolysis reaction to generate xylo-oligosaccharide, cooling the xylo-oligosaccharide after the enzymolysis reaction, and then obtaining a hydrolysis mixed solution;
(4) and separating and purifying
Performing solid-liquid classification on the hydrolysis mixed solution to obtain a filtrate which is the xylo-oligosaccharide hydrolysis sugar solution, and then filtering the part of the xylo-oligosaccharide hydrolysis sugar solution by adopting an ultrafiltration membrane to obtain the filtrate of the xylo-oligosaccharide hydrolysis sugar solution;
(5) concentrating and purifying
Concentrating the filtrate of the xylo-oligosaccharide hydrolyzed sugar solution by using an evaporator, and purifying to obtain high-purity xylo-oligosaccharide syrup liquid after concentration;
(6) and crystal preparation
And finally, carrying out vacuum drying on the high-purity xylo-oligosaccharide syrup liquid concentrated by the vacuum dryer to obtain crystalline xylose, thus obtaining the finished feed-grade xylo-oligosaccharide.
3. The method for preparing feed-grade xylo-oligosaccharide from corncobs as claimed in claim 2, wherein the method comprises the following steps: in the corn cob pulping process in the step (2), the ratio of the corn cob powder to the distilled water is 1: 10.
4. The method for preparing feed-grade xylo-oligosaccharide from corncobs as claimed in claim 2, wherein the method comprises the following steps: and (3) in the corncob pulping process in the step (2), the stirring speed of the stirring equipment is 60-80 r/m.
5. The method for preparing feed-grade xylo-oligosaccharide from corncobs as claimed in claim 2, wherein the method comprises the following steps: in the corn cob pulping process in the step (2), the cooking temperature of the corn cob powder slurry is 90-100 ℃, and the cooking time is 1-2 h.
6. The method for preparing feed-grade xylo-oligosaccharide from corncobs as claimed in claim 2, wherein the method comprises the following steps: the complex enzyme in the enzymolysis treatment process in the step (3) is prepared by mixing xylanase and cellulase, and the ratio of the xylanase to the cellulase is 2: 1.
7. The method for preparing feed-grade xylo-oligosaccharide from corncobs as claimed in claim 2, wherein the method comprises the following steps: the enzymolysis temperature of the slurry in the enzymolysis treatment process in the step (3) is 50-60 ℃, and the enzymolysis time is 1-1.5 h.
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Cited By (2)
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| CN114539332A (en) * | 2022-01-10 | 2022-05-27 | 长沙绿叶生物科技有限公司 | Method for preparing xylooligosaccharide by using biomass raw material and application of xylooligosaccharide in preparation of feed additive |
| CN115044629A (en) * | 2022-05-25 | 2022-09-13 | 南京林业大学 | Method for producing corncob feed raw material containing xylo-oligosaccharide |
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Cited By (3)
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| CN115044629A (en) * | 2022-05-25 | 2022-09-13 | 南京林业大学 | Method for producing corncob feed raw material containing xylo-oligosaccharide |
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