CN114836584B - Method for producing xylo-oligosaccharide with assistance of amino acid - Google Patents
Method for producing xylo-oligosaccharide with assistance of amino acid Download PDFInfo
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- CN114836584B CN114836584B CN202210567065.9A CN202210567065A CN114836584B CN 114836584 B CN114836584 B CN 114836584B CN 202210567065 A CN202210567065 A CN 202210567065A CN 114836584 B CN114836584 B CN 114836584B
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- amino acid
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- xylan
- oligosaccharide
- xylo
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- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 title claims abstract description 35
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 30
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 15
- 229920001221 xylan Polymers 0.000 claims abstract description 23
- 150000004823 xylans Chemical class 0.000 claims abstract description 23
- 230000007062 hydrolysis Effects 0.000 claims abstract description 21
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 21
- 239000002994 raw material Substances 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 230000002378 acidificating effect Effects 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 3
- 238000000926 separation method Methods 0.000 claims abstract description 3
- 238000006243 chemical reaction Methods 0.000 claims description 31
- 235000001014 amino acid Nutrition 0.000 claims description 24
- 239000000203 mixture Substances 0.000 claims description 16
- 238000003756 stirring Methods 0.000 claims description 13
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 9
- 238000001125 extrusion Methods 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 9
- 235000013922 glutamic acid Nutrition 0.000 claims description 9
- 239000004220 glutamic acid Substances 0.000 claims description 9
- 238000007789 sealing Methods 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 9
- 229910001220 stainless steel Inorganic materials 0.000 claims description 9
- 239000010935 stainless steel Substances 0.000 claims description 9
- 239000002028 Biomass Substances 0.000 claims description 8
- 238000010907 mechanical stirring Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 5
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 3
- 235000003704 aspartic acid Nutrition 0.000 claims description 3
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims 1
- 238000001816 cooling Methods 0.000 claims 1
- 238000011068 loading method Methods 0.000 claims 1
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 abstract description 23
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 abstract description 22
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 abstract description 14
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 abstract description 14
- 239000000047 product Substances 0.000 abstract description 12
- 239000006227 byproduct Substances 0.000 abstract description 5
- 239000003674 animal food additive Substances 0.000 abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 3
- 150000007522 mineralic acids Chemical class 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 15
- 239000000413 hydrolysate Substances 0.000 description 9
- 238000005571 anion exchange chromatography Methods 0.000 description 8
- 241000609240 Ambelania acida Species 0.000 description 7
- 229920002488 Hemicellulose Polymers 0.000 description 7
- 239000010905 bagasse Substances 0.000 description 7
- 125000000969 xylosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)CO1)* 0.000 description 5
- FTTUBRHJNAGMKL-UHFFFAOYSA-N Xylohexaose Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(OC4C(C(O)C(OC5C(C(O)C(O)OC5)O)OC4)O)OC3)O)OC2)O)OC1 FTTUBRHJNAGMKL-UHFFFAOYSA-N 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 239000003513 alkali Substances 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- LGQKSQQRKHFMLI-SJYYZXOBSA-N (2s,3r,4s,5r)-2-[(3r,4r,5r,6r)-4,5,6-trihydroxyoxan-3-yl]oxyoxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)OC1 LGQKSQQRKHFMLI-SJYYZXOBSA-N 0.000 description 3
- LGQKSQQRKHFMLI-UHFFFAOYSA-N 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(O)OC1 LGQKSQQRKHFMLI-UHFFFAOYSA-N 0.000 description 3
- 235000018185 Betula X alpestris Nutrition 0.000 description 3
- 235000018212 Betula X uliginosa Nutrition 0.000 description 3
- SQNRKWHRVIAKLP-UHFFFAOYSA-N D-xylobiose Natural products O=CC(O)C(O)C(CO)OC1OCC(O)C(O)C1O SQNRKWHRVIAKLP-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000010902 straw Substances 0.000 description 3
- JCSJTDYCNQHPRJ-UHFFFAOYSA-N 20-hydroxyecdysone 2,3-acetonide Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(OC2C(C(O)C(O)OC2)O)OC1 JCSJTDYCNQHPRJ-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 229920002522 Wood fibre Polymers 0.000 description 2
- JVZHSOSUTPAVII-UHFFFAOYSA-N Xylotetraose Natural products OCC(OC1OCC(OC2OCC(OC3OCC(O)C(O)C3O)C(O)C2O)C(O)C1O)C(O)C(O)C=O JVZHSOSUTPAVII-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- JCSJTDYCNQHPRJ-FDVJSPBESA-N beta-D-Xylp-(1->4)-beta-D-Xylp-(1->4)-D-Xylp Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)C(O)OC2)O)OC1 JCSJTDYCNQHPRJ-FDVJSPBESA-N 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000002025 wood fiber Substances 0.000 description 2
- KPTPSLHFVHXOBZ-BIKCPUHGSA-N xylotetraose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O[C@H]3[C@@H]([C@@H](O)C(O)OC3)O)OC2)O)OC1 KPTPSLHFVHXOBZ-BIKCPUHGSA-N 0.000 description 2
- ABKNGTPZXRUSOI-UHFFFAOYSA-N xylotriose Natural products OCC(OC1OCC(OC2OCC(O)C(O)C2O)C(O)C1O)C(O)C(O)C=O ABKNGTPZXRUSOI-UHFFFAOYSA-N 0.000 description 2
- 108010001817 Endo-1,4-beta Xylanases Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- LFFQNKFIEIYIKL-UHFFFAOYSA-N Xylopentaose Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(OC4C(C(O)C(O)OC4)O)OC3)O)OC2)O)OC1 LFFQNKFIEIYIKL-UHFFFAOYSA-N 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 150000008064 anhydrides Chemical class 0.000 description 1
- 238000003975 animal breeding Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K13/00—Sugars not otherwise provided for in this class
- C13K13/002—Xylose
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0057—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Xylans, i.e. xylosaccharide, e.g. arabinoxylan, arabinofuronan, pentosans; (beta-1,3)(beta-1,4)-D-Xylans, e.g. rhodymenans; Hemicellulose; Derivatives thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Sustainable Development (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The application discloses a method for producing xylooligosaccharide by amino acid assistance, which comprises the steps of raw material hydrolysis, solid-liquid separation and product drying preparation, wherein acidic amino acid is added in the raw material hydrolysis step. According to the application, the xylan hydrolysis is promoted by adding acidic amino acid, and compared with inorganic acid, the produced xylan is not easy to excessively degrade, the yield is high, and byproducts of xylose and furfural are less. The application selects amino acid as the auxiliary hydrolysis reagent of xylan, and can be directly and jointly mixed with xylo-oligosaccharide to prepare the feed additive containing amino acid type xylo-oligosaccharide. The application has simple production process and green product, and can be used for various xylan raw materials.
Description
Technical Field
The application belongs to the technical field of comprehensive utilization of agriculture and forestry biomass, relates to a preparation technology of xylo-oligosaccharide, and in particular relates to a method for producing xylo-oligosaccharide by amino acid assistance.
Background
The xylooligosaccharide is used as a non-digestible oligosaccharide and a functional food or feed additive, is a super-strong prebiotic, and can activate various immune cell activities through probiotics in intestinal tracts; under the traction and drive of the high-speed development of industries such as green animal breeding and health care, ecological agriculture and the like, the development prospect of xylooligosaccharide products derived from agriculture and forestry biomass is wide. At present, the main production method of xylooligosaccharide is that endo xylanase is adopted for hydrolysis after xylan is dissolved by alkali, and the method has the advantages of more waste water, large pollution and complex process; in addition, the ordinary strong acid hydrolysis method can be adopted to prepare the xylo-oligosaccharide, the xylo-oligosaccharide yield is low, byproducts are more, and the product quality is not high.
Disclosure of Invention
Aiming at the defects existing in the prior art, the technical problem to be solved by the application is to provide the method for producing the xylo-oligosaccharide by amino acid assistance, which has the technical advantages of high xylo-oligosaccharide yield, less byproduct xylose and furfural and the like.
In order to solve the technical problems, the application adopts the following technical scheme:
a method for producing xylo-oligosaccharide with the assistance of amino acid, which comprises the steps of raw material hydrolysis, solid-liquid separation, product drying and the like; acidic amino acid is added in the step of preparing sugar solution rich in xylooligosaccharide by hydrolyzing the agriculture and forestry biomass raw material or the xylan raw material.
The amino acid is glutamic acid and/or aspartic acid.
The method for producing the xylo-oligosaccharide by the aid of the amino acid is characterized in that heating and stirring are carried out in the hydrolysis preparation, the stirring speed is 30-100 rmp, the temperature is 140-170 ℃, and the time is 0.15-2.0 h.
In the method for producing the xylooligosaccharide by the aid of the amino acid, the mass fraction of the added amino acid is 1-5%.
In the method for producing the xylooligosaccharide by the aid of the amino acid, a xylan raw material and an acidic glutamic acid solution are added into a mechanically stirred stainless steel high-pressure reaction tank, and after sealing, stirring is started at 120rpm, and the mixture is heated to 150 ℃ and kept for 50min; after the reaction is finished, the reactor tank is cooled to room temperature, the reacted solid-liquid mixture is filled into a vacuum pulp washer, and the solid matters which are not hydrolyzed and hydrolyzed sugar liquid are separated through extrusion and filtration, and the hydrolyzed sugar liquid is the sugar liquid which is rich in xylooligosaccharide and is prepared.
The raw material is selected from an agricultural and forestry biomass raw material rich in xylan or a xylan raw material. Such as agricultural and forestry biomass, lignocellulosic feedstocks including alkali extracted xylan, straw, corn cob, bagasse, and the like.
The beneficial effects are that: compared with the prior art, the application has the advantages that:
1) According to the application, the xylan hydrolysis is promoted by adding acidic amino acid, and compared with inorganic acid, the produced xylan is not easy to excessively degrade, the yield is high, and byproducts of xylose and furfural are less.
2) The application selects amino acid as the auxiliary hydrolysis reagent of xylan, and can be directly and jointly mixed with xylo-oligosaccharide to prepare the feed additive containing amino acid type xylo-oligosaccharide.
3) The application has simple production process and green products, and can be used for various xylan raw materials (agriculture and forestry biomass, wood fiber raw materials, including alkali extraction xylan, straw, corncob, bagasse and the like).
Drawings
FIG. 1 is a chart of analysis of the product of example 4, in which Xylose: xylose; x2: xylobiose; x3: xylotriose; x4: xylotetraose; x5: wood five ponds; x6: a xylohexose; GA: glutamic acid.
Detailed Description
The application will be further illustrated with reference to specific examples.
The following examples analyze the sugar components in the product using high performance anion exchange chromatography, chromatographic conditions: siemens Fei ICS5000 ion chromatography, configuration of CarboPac TM PA200 (3 mm. Times.250 mm) chromatographic column, PAD integral amperometric detector, column temperature 30 ℃, sample injection volume 10. Mu.L; binary gradient leaching is carried out by taking 100mmol/L sodium hydroxide and 500mmol/L sodium acetate as mobile phases, and the flow rate is 0.3mL/min.
Example 1
1000g of crushed corncob and 0.75 mass percent of sulfuric acid solution 5L are added into a 10L mechanical stirring type stainless steel high-pressure reaction tank, stirring (60 rpm) is started after sealing, and the mixture is heated to 160 ℃ and kept for 25min; after the reaction is finished, the solid-liquid mixture after the reaction is cooled to room temperature in a reaction tank is filled into a vacuum pulp washer, and the solid which is not hydrolyzed and the corn cob hemicellulose hydrolysis sugar solution are separated through extrusion and filtration. The sugar component of the obtained hydrolysate sample (product 1) is analyzed by adopting high-efficiency anion exchange chromatography, and the result is shown in table 1, wherein the main components of the hydrolysate sample are Xylose to xylohexaose (Xylose), xylobiose (X2), xylotriose (X3), xylotetraose (X4), xylopentaose (X5) and xylohexaose (X6)), and the total amount of the xylooligosaccharide (total amount of xylobiose to xylohexaose) is 81.2 percent; in addition, the yield of furfural is 2.1%.
Example 2
Adding 1000g of crushed corncob and 5L of pure water into a 10L mechanical stirring type stainless steel high-pressure reaction tank, sealing, starting stirring (60 rpm), heating to 180 ℃ and preserving heat for 50min; after the reaction is finished, the solid-liquid mixture after the reaction is cooled to room temperature in a reaction tank is filled into a vacuum pulp washer, and the solid which is not hydrolyzed and the corn cob hemicellulose hydrolysis sugar solution are separated through extrusion and filtration. The obtained hydrolysate sample adopts high-efficiency anion exchange chromatography to analyze the sugar component, the result is shown in table 1, the main component is xylose to xylohexase, the total is 35.9%, and the total amount of xylooligosaccharide is 24.8%; in addition, the yield of the furfural is 0.8%.
Example 3
1000g of crushed corncob and 2.5 mass percent of glutamic acid solution 5L are added into a 10L mechanical stirring type stainless steel high-pressure reaction tank, stirring (60 rpm) is started after sealing, and the mixture is heated to 160 ℃ and kept for 45min; after the reaction is finished, the solid-liquid mixture after the reaction is cooled to room temperature in a reaction tank is filled into a vacuum pulp washer, and the solid which is not hydrolyzed and the corn cob hemicellulose hydrolysis sugar solution are separated through extrusion and filtration. The obtained hydrolysate sample adopts high-efficiency anion exchange chromatography to analyze the sugar component, the result is shown in table 1, the main component is xylose to xylohexase, the total is 79.5%, and the total amount of xylooligosaccharide is 50.7%; in addition, the yield of the furfural is 0.4%.
Example 4
Adding 1000g of betulinic anhydride and 5% (mass fraction) of glutamic acid solution 5L into a 10L mechanical stirring stainless steel high-pressure reaction tank, sealing, stirring (120 rpm), heating to 150 ℃, and preserving heat for 50min; after the reaction is finished, the solid-liquid mixture after the reaction is cooled to room temperature in a reaction tank is filled into a vacuum pulp washer, and the solid which is not hydrolyzed and the birch hemicellulose hydrolysis sugar solution are separated through extrusion and filtration. The obtained hydrolysate sample adopts high-efficiency anion exchange chromatography to analyze the sugar component, the analysis chart is shown in figure 1, the result is shown in table 1, the main component is xylose to xylohexaose, the total is 85.1%, and the total amount of xylooligosaccharide is 53.4%; in addition, the yield of the furfural is 0.5%.
Example 5
Adding 1000g of birch xylan and 5L of 6% (mass fraction) aspartic acid solution into a 10L mechanically-stirred stainless steel high-pressure reaction tank, sealing, stirring (100 rpm) and heating to 170 ℃ for 25min; after the reaction is finished, the solid-liquid mixture after the reaction is cooled to room temperature in a reaction tank is filled into a vacuum pulp washer, and the solid which is not hydrolyzed and the birch hemicellulose hydrolysis sugar solution are separated through extrusion and filtration. The obtained hydrolysate sample adopts high-efficiency anion exchange chromatography to analyze the sugar component, the result is shown in table 1, the main component is xylose to xylohexase, the total is 83.5%, and the total amount of xylooligosaccharide is 50.4%; in addition, the yield of the furfural is 0.8%.
Example 6
1000g of crushed bagasse and 5 percent (mass fraction) of glutamic acid solution 5L are added into a 10L mechanical stirring type stainless steel high-pressure reaction tank, stirring (60 rpm) is started after sealing, and the mixture is heated to 110 ℃ and kept for 70 minutes; after the reaction is finished, the solid-liquid mixture after the reaction is cooled to room temperature in a reactor tank is filled into a vacuum pulp washer, and the solid which is not hydrolyzed and bagasse hemicellulose hydrolysis sugar solution are separated through extrusion and filtration. The obtained hydrolysate sample adopts high-efficiency anion exchange chromatography to analyze the sugar component, the result is shown in table 1, the main component is xylose to xylohexase, the total is 38.1%, and the total amount of xylooligosaccharide is 24.5%; in addition, the yield of the furfural is 0.6%.
Example 7
1000g of crushed bagasse and 5 percent (mass fraction) of glutamic acid solution 5L are added into a 10L mechanical stirring type stainless steel high-pressure reaction tank, stirring (60 rpm) is started after sealing, and the mixture is heated to 170 ℃ and kept for 40 minutes; after the reaction is finished, the solid-liquid mixture after the reaction is cooled to room temperature in a reactor tank is filled into a vacuum pulp washer, and the solid which is not hydrolyzed and bagasse hemicellulose hydrolysis sugar solution are separated through extrusion and filtration. The sugar component of the obtained hydrolysate sample (product 7) is analyzed by adopting high-efficiency anion exchange chromatography, the result is shown in table 1, the main component of the hydrolysate sample is 82.1 percent of xylose to xylohexasaccharide, and the total amount of xylooligosaccharide is 51.3 percent; in addition, the yield of the furfural is 0.6%.
TABLE 1 results Table of the product compositions
As shown in the results of Table 1, the method helps to promote the hydrolysis of xylan by adding acidic amino acid, and compared with inorganic acid, the produced xylan is not easy to excessively degrade, the yield is high, and byproducts, namely xylose and furfural are few; the method has technical universality and can be used for various xylan raw materials (agriculture and forestry biomass, wood fiber raw materials, including alkali extraction xylan, straw, corncob, bagasse and the like); the type, amount, time and temperature of the amino acid in the application need to be strictly controlled. The treatment time of the amino acid concentration is too low, and the energy consumption is high; excessive degradation yield reduction easily occurs to products with excessively high temperature and time; suitable conditions and proportions are required.
The application selects amino acid as the auxiliary hydrolysis reagent of xylan, and can be directly and jointly mixed with xylo-oligosaccharide to prepare the feed additive containing amino acid type xylo-oligosaccharide.
Claims (3)
1. The method for producing the xylo-oligosaccharide by amino acid assistance comprises the steps of raw material hydrolysis, solid-liquid separation and product drying, and is characterized in that: an acidic amino acid is added in the raw material hydrolysis step; the amino acid is glutamic acid and/or aspartic acid; heating and stirring in the hydrolysis step, wherein the stirring speed is 30-100 rpm, the temperature is 140-170 ℃ and the time is 0.15-2.0 h; the mass fraction of the added amino acid is 1-5%.
2. The method for producing xylooligosaccharide by amino acid assistance according to claim 1, wherein the method comprises the following steps: the raw material is selected from an agricultural and forestry biomass raw material rich in xylan or a xylan raw material.
3. A method for producing xylo-oligosaccharide with the assistance of amino acid is characterized in that: 1000g of raw material and 5L of 5% acid glutamic acid solution by mass fraction are added into a 10L mechanical stirring type stainless steel high-pressure reaction tank, stirring is started after sealing, and the mixture is heated to 150 ℃ and kept for 50min; after the reaction is finished, cooling the reaction tank to room temperature, loading the reacted solid-liquid mixture into a vacuum pulp washer, and separating unhydrolyzed solid and hydrolyzed sugar liquid by extrusion and filtration, wherein the hydrolyzed sugar liquid is the prepared sugar liquid rich in xylooligosaccharide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210567065.9A CN114836584B (en) | 2022-05-23 | 2022-05-23 | Method for producing xylo-oligosaccharide with assistance of amino acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210567065.9A CN114836584B (en) | 2022-05-23 | 2022-05-23 | Method for producing xylo-oligosaccharide with assistance of amino acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114836584A CN114836584A (en) | 2022-08-02 |
| CN114836584B true CN114836584B (en) | 2023-12-01 |
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