CN116751235A - Method for producing xylo-oligosaccharide through aspartic acid-assisted steam explosion treatment - Google Patents
Method for producing xylo-oligosaccharide through aspartic acid-assisted steam explosion treatment Download PDFInfo
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- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 title claims abstract description 63
- 238000004880 explosion Methods 0.000 title claims abstract description 56
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 title claims abstract description 54
- 235000003704 aspartic acid Nutrition 0.000 title claims abstract description 54
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 title claims abstract description 54
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 34
- 239000007788 liquid Substances 0.000 claims abstract description 95
- 239000000203 mixture Substances 0.000 claims abstract description 30
- 238000000926 separation method Methods 0.000 claims abstract description 29
- 238000002156 mixing Methods 0.000 claims abstract description 28
- 239000000463 material Substances 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 20
- 229920001221 xylan Polymers 0.000 claims abstract description 18
- 150000004823 xylans Chemical class 0.000 claims abstract description 18
- 238000003756 stirring Methods 0.000 claims abstract description 14
- 238000010438 heat treatment Methods 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 239000007787 solid Substances 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 238000003825 pressing Methods 0.000 claims description 12
- 241000609240 Ambelania acida Species 0.000 claims description 10
- 239000010905 bagasse Substances 0.000 claims description 10
- 239000012153 distilled water Substances 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 238000001125 extrusion Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 2
- 230000035484 reaction time Effects 0.000 claims description 2
- 239000004065 semiconductor Substances 0.000 claims description 2
- 239000011343 solid material Substances 0.000 claims description 2
- 239000003054 catalyst Substances 0.000 abstract description 2
- JCSJTDYCNQHPRJ-UHFFFAOYSA-N 20-hydroxyecdysone 2,3-acetonide Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(OC2C(C(O)C(O)OC2)O)OC1 JCSJTDYCNQHPRJ-UHFFFAOYSA-N 0.000 description 11
- LGQKSQQRKHFMLI-UHFFFAOYSA-N 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(O)OC1 LGQKSQQRKHFMLI-UHFFFAOYSA-N 0.000 description 11
- SQNRKWHRVIAKLP-UHFFFAOYSA-N D-xylobiose Natural products O=CC(O)C(O)C(CO)OC1OCC(O)C(O)C1O SQNRKWHRVIAKLP-UHFFFAOYSA-N 0.000 description 11
- FTTUBRHJNAGMKL-UHFFFAOYSA-N Xylohexaose Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(OC4C(C(O)C(OC5C(C(O)C(O)OC5)O)OC4)O)OC3)O)OC2)O)OC1 FTTUBRHJNAGMKL-UHFFFAOYSA-N 0.000 description 11
- LFFQNKFIEIYIKL-UHFFFAOYSA-N Xylopentaose Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(OC4C(C(O)C(O)OC4)O)OC3)O)OC2)O)OC1 LFFQNKFIEIYIKL-UHFFFAOYSA-N 0.000 description 11
- JVZHSOSUTPAVII-UHFFFAOYSA-N Xylotetraose Natural products OCC(OC1OCC(OC2OCC(OC3OCC(O)C(O)C3O)C(O)C2O)C(O)C1O)C(O)C(O)C=O JVZHSOSUTPAVII-UHFFFAOYSA-N 0.000 description 11
- JCSJTDYCNQHPRJ-FDVJSPBESA-N beta-D-Xylp-(1->4)-beta-D-Xylp-(1->4)-D-Xylp Chemical group O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)C(O)OC2)O)OC1 JCSJTDYCNQHPRJ-FDVJSPBESA-N 0.000 description 11
- KPTPSLHFVHXOBZ-BIKCPUHGSA-N xylotetraose Chemical group O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O[C@H]3[C@@H]([C@@H](O)C(O)OC3)O)OC2)O)OC1 KPTPSLHFVHXOBZ-BIKCPUHGSA-N 0.000 description 11
- ABKNGTPZXRUSOI-UHFFFAOYSA-N xylotriose Natural products OCC(OC1OCC(OC2OCC(O)C(O)C2O)C(O)C1O)C(O)C(O)C=O ABKNGTPZXRUSOI-UHFFFAOYSA-N 0.000 description 11
- LGQKSQQRKHFMLI-SJYYZXOBSA-N (2s,3r,4s,5r)-2-[(3r,4r,5r,6r)-4,5,6-trihydroxyoxan-3-yl]oxyoxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)OC1 LGQKSQQRKHFMLI-SJYYZXOBSA-N 0.000 description 10
- 238000005571 anion exchange chromatography Methods 0.000 description 10
- 238000005422 blasting Methods 0.000 description 10
- 230000007062 hydrolysis Effects 0.000 description 4
- 238000006460 hydrolysis reaction Methods 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000000184 acid digestion Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 229920002522 Wood fibre Polymers 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 239000002025 wood fiber Substances 0.000 description 1
- 125000002258 xylobiose group Chemical group 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The application discloses a method for producing xylooligosaccharide by aspartic acid-assisted steam explosion treatment, which comprises the steps of crushing a raw material rich in xylan to prepare a crushed material; mixing the crushed material with an aspartic acid solution, and uniformly stirring to obtain an aspartic acid-containing material; heating the material containing aspartic acid in a steam explosion reactor by steam, and instantly releasing pressure to obtain a solid-liquid mixture; and carrying out solid-liquid separation on the solid-liquid mixture to obtain the xylooligosaccharide liquid. The application provides a method for producing xylo-oligosaccharide by using aspartic acid as a catalyst and combining steam explosion physical structure, wherein the method is used for producing xylo-oligosaccharide by using aspartic acid admitted by feed.
Description
Technical Field
The application belongs to the field of xylo-oligosaccharide production, and particularly relates to a method for producing xylo-oligosaccharide through steam explosion treatment assisted by aspartic acid.
Background
Xylo-oligosaccharide is also called xylo-oligosaccharide, and is a functional polymeric sugar formed by combining 2-7 xylose molecules through beta-1, 4 glycosidic bonds.
At present, the production method of xylo-oligosaccharide mainly comprises the steps of alkali extraction xylan combined enzyme hydrolysis, steam explosion pretreatment combined enzyme hydrolysis and acid digestion one-step hydrolysis. The product preparation in the production process of the combination method has higher requirements on xylanase preparations, and the process is relatively complex and has high cost; the acid digestion method mainly aims at solving the problems of large acid consumption and high product purification difficulty.
At present, the main production method of the xylo-oligosaccharide (polymerization degree is 2-10) is a set of alkaline pretreatment, xylan purification and biological enzyme hydrolysis, and the method has the problems of high production purity of the xylo-oligosaccharide, complex process, high cost and large pollution of high-salt wastewater.
Therefore, there is a need in the art for a method for preparing xylo-oligosaccharide with simple process and high yield.
Disclosure of Invention
This section is intended to outline some aspects of embodiments of the application and to briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section as well as in the description of the application and in the title of the application, which may not be used to limit the scope of the application.
The present application has been made in view of the above and/or problems occurring in the prior art.
Therefore, the application aims to overcome the defects in the prior art and provide a method for producing xylo-oligosaccharide by aspartic acid-assisted steam explosion treatment.
In order to solve the technical problems, the application provides the following technical scheme: a method for producing xylo-oligosaccharide by gas explosion treatment assisted by aspartic acid comprises,
crushing the raw material rich in xylan to obtain a crushed material;
mixing the crushed material with an aspartic acid solution, and uniformly stirring to obtain an aspartic acid-containing material;
heating the material containing aspartic acid in a steam explosion reactor by steam, and instantly releasing pressure to obtain a solid-liquid mixture;
and carrying out solid-liquid separation on the solid-liquid mixture to obtain the xylooligosaccharide liquid.
As a preferable scheme of the method for producing the xylo-oligosaccharide by the aspartic acid-assisted steam explosion treatment, the application comprises the following steps: also included is a method of manufacturing a semiconductor device,
and (3) adding distilled water into the solid obtained by solid-liquid separation of the solid-liquid mixture, uniformly stirring, and carrying out solid-liquid separation again to obtain the xylooligosaccharide liquid.
As a preferable scheme of the method for producing the xylo-oligosaccharide by the aspartic acid-assisted steam explosion treatment, the application comprises the following steps: the xylan-rich raw material comprises corncob and bagasse.
As a preferable scheme of the method for producing the xylo-oligosaccharide by the aspartic acid-assisted steam explosion treatment, the application comprises the following steps: the particle size of the crushed material is 0.1-10 mm.
As a preferable scheme of the method for producing the xylo-oligosaccharide by the aspartic acid-assisted steam explosion treatment, the application comprises the following steps: the mass fraction of aspartic acid in the aspartic acid solution is 0.1-2.0%.
As a preferable scheme of the method for producing the xylo-oligosaccharide by the aspartic acid-assisted steam explosion treatment, the application comprises the following steps: the mass ratio of the crushed material to the aspartic acid solution is 1:0.5-3.5.
As a preferable scheme of the method for producing the xylo-oligosaccharide by the aspartic acid-assisted steam explosion treatment, the application comprises the following steps: the reaction temperature of the steam explosion is 160-200 ℃, the reaction pressure is 0.6-2.0 MPa, and the reaction time is 3-20 min.
As a preferable scheme of the method for producing the xylo-oligosaccharide by the aspartic acid-assisted steam explosion treatment, the application comprises the following steps: the solid-liquid separation comprises plate-frame filter pressing and extrusion filtering.
As a preferable scheme of the method for producing the xylo-oligosaccharide by the aspartic acid-assisted steam explosion treatment, the application comprises the following steps: and adding distilled water into the solid obtained by solid-liquid separation of the solid-liquid mixture, wherein the mass of the distilled water is 3-10 times of that of the solid material.
As a preferable scheme of the method for producing the xylo-oligosaccharide by the aspartic acid-assisted steam explosion treatment, the application comprises the following steps: the solid-liquid separation comprises plate-frame filter pressing and extrusion filtering.
The application has the beneficial effects that:
(1) The application provides a method for producing xylo-oligosaccharide by using aspartic acid as a catalyst and combining steam explosion physical structure, wherein the method is used for producing xylo-oligosaccharide by using aspartic acid admitted by feed.
(2) The method has technical universality and can be used for extracting xylan, straw, corncob, bagasse and the like from various wood fiber raw materials; in addition, the acid dosage, the blasting time and the temperature in the application need to be strictly controlled to achieve the preset effect.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art. Wherein:
FIG. 1 is a flow chart of a process for producing xylo-oligosaccharide by aspartic acid-assisted steam explosion treatment in an embodiment of the application.
Fig. 2 is a detection chart of ion chromatography of main components of an xylooligosaccharide liquid produced by aspartic acid-assisted steam explosion, wherein X2 is xylobiose, X3 is xylotriose, X4 is xylotetraose, X5 is xylopentaose, X6 is xylohexaose, X7 is xyloheptaose, X8 is xylooctaose, X9 is xylononaose and X10 is xylodecaose.
Detailed Description
In order that the above-recited objects, features and advantages of the present application will become more apparent, a more particular description of the application will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application, but the present application may be practiced in other ways other than those described herein, and persons skilled in the art will readily appreciate that the present application is not limited to the specific embodiments disclosed below.
Further, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic can be included in at least one implementation of the application. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
Example 1
Crushing 10kg of corncob (30% of xylan content) to 0.5-3mm, then sending into a reactor of a steam explosion machine, introducing supersaturated steam, heating to 180 ℃, maintaining the pressure at 1.0MPa for 10min, instantaneously releasing the pressure, explosion the wet material into a receiver, and collecting a solid-liquid mixture subjected to steam explosion treatment;
separating the solid-liquid mixture by a plate-and-frame filter press, and respectively collecting liquid and solid parts;
the liquid part is xylooligosaccharide liquid, and the mass of the total xylooligosaccharide (xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose, xyloheptaose, xylooctaose, xylononaose and xylodecaose) is 0.3kg detected by a high-efficiency anion exchange chromatography, and the yield is 10%.
Example 2
Crushing 10kg of corncob (30% of xylan content) to 0.5-3mm, uniformly mixing and stirring with 0.5% of aspartic acid solution according to a mass ratio of 1:2, feeding into a steam explosion machine reactor, introducing supersaturated steam, heating to 180 ℃, maintaining the pressure at 1.0MPa for 10min, instantaneously releasing the pressure, blasting wet materials into a receiver, and collecting a solid-liquid mixture subjected to steam explosion treatment;
separating the solid-liquid mixture by a plate-and-frame filter press, and respectively collecting liquid and solid parts;
the liquid part is xylooligosaccharide liquid, and the mass of the total xylooligosaccharide (xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose, xyloheptaose, xylooctaose, xylononaose and xylodecaose) is 0.7kg detected by a high-efficiency anion exchange chromatography, and the yield is 23%.
Example 3
Crushing 10kg of corncob (30% of xylan content) to 0.5-3mm, uniformly mixing and stirring with 0.5% of aspartic acid solution according to a mass ratio of 1:2, feeding into a steam explosion machine reactor, introducing supersaturated steam, heating to 180 ℃, maintaining the pressure at 1.0MPa for 10min, instantaneously releasing the pressure, blasting wet materials into a receiver, and collecting a solid-liquid mixture subjected to steam explosion treatment;
separating the solid-liquid mixture by a plate-and-frame filter press, and respectively collecting liquid and solid parts;
and uniformly mixing the solid part with 50kg of water, then carrying out plate-frame filter pressing separation again, collecting liquid, and mixing the liquid obtained by the two solid-liquid separation into xylooligosaccharide liquid, wherein the specific flow is shown in figure 1.
The quality of the total xylo-oligosaccharide (xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose, xyloheptaose, xylooctaose, xylononaose and xylodecaose) is detected to be 1.3kg by a high-efficiency anion exchange chromatography, the yield is 43%, and the analysis chart of the product is shown in figure 2.
Example 4
Crushing 10kg of bagasse (with the xylan content of 24%) to a size of 0.5-3mm, uniformly mixing and stirring with an aspartic acid solution with the concentration of 0.5% according to a mass ratio of 1:2, feeding into a reactor of a steam explosion machine, introducing supersaturated steam, heating to 200 ℃, maintaining the pressure at 1.5MPa for 7min, instantaneously releasing the pressure, blasting wet materials into a receiver, and collecting a solid-liquid mixture subjected to steam explosion treatment;
separating the solid-liquid mixture by a plate-and-frame filter press, and respectively collecting liquid and solid parts;
mixing the solid part with 50kg of water uniformly, then carrying out plate-frame filter pressing separation again, collecting liquid, and mixing the liquid obtained by the two solid-liquid separation to obtain xylooligosaccharide liquid.
The quality of the total xylooligosaccharide (xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose, xyloheptaose, xylooctaose, xylononaose and xylodecaose) is 1.1kg detected by a high-efficiency anion exchange chromatography, and the yield is 46%.
Example 5
Crushing 10kg of bagasse (with the xylan content of 24%) to a size of 0.5-3mm, uniformly mixing and stirring with an aspartic acid solution with the concentration of 0.5% according to a mass ratio of 1:2, feeding into a reactor of a steam explosion machine, introducing supersaturated steam, heating to 200 ℃, maintaining the pressure at 1.5MPa for 7min, instantaneously releasing the pressure, blasting wet materials into a receiver, and collecting a solid-liquid mixture subjected to steam explosion treatment;
separating the solid-liquid mixture by a plate-and-frame filter press, and respectively collecting liquid and solid parts;
uniformly mixing the solid part with 30kg of water, then carrying out plate-frame filter pressing separation again, collecting liquid, and mixing the liquid obtained by the two solid-liquid separation to obtain xylooligosaccharide liquid.
The quality of the total xylooligosaccharide (xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose, xyloheptaose, xylooctaose, xylononaose and xylodecaose) is detected to be 0.98kg by a high-efficiency anion exchange chromatography, and the yield is 41%.
Example 6
Crushing 10kg of bagasse (with the xylan content of 24%) to a size of 0.5-3mm, uniformly mixing and stirring with an aspartic acid solution with the concentration of 0.75% according to a mass ratio of 1:2, feeding into a reactor of a steam explosion machine, introducing supersaturated steam, heating to 190 ℃, maintaining the pressure at 1.3MPa for 7min, instantaneously releasing the pressure, blasting wet materials into a receiver, and collecting a solid-liquid mixture subjected to steam explosion treatment;
separating the solid-liquid mixture by a plate-and-frame filter press, and respectively collecting liquid and solid parts;
mixing the solid part with 50kg of water uniformly, then carrying out plate-frame filter pressing separation again, collecting liquid, and mixing the liquid obtained by the two solid-liquid separation to obtain xylooligosaccharide liquid.
The quality of the total xylooligosaccharide (xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose, xyloheptaose, xylooctaose, xylononaose and xylodecaose) is 1.2kg detected by a high-efficiency anion exchange chromatography, and the yield is 50%.
Example 7
Crushing 10kg of bagasse (with the xylan content of 24%) to a size of 0.5-3mm, uniformly mixing and stirring with 0.8% glutamic acid solution according to a mass ratio of 1:2, then sending into a reactor of a steam explosion machine, introducing supersaturated steam, heating to 190 ℃, maintaining the pressure at 1.3MPa for 7min, instantaneously releasing the pressure, blasting wet materials into a receiver, and collecting a solid-liquid mixture subjected to steam explosion treatment;
separating the solid-liquid mixture by a plate-and-frame filter press, and respectively collecting liquid and solid parts;
mixing the solid part with 50kg of water uniformly, then carrying out plate-frame filter pressing separation again, collecting liquid, and mixing the liquid obtained by the two solid-liquid separation to obtain xylooligosaccharide liquid.
The quality of the total xylooligosaccharide (xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose, xyloheptaose, xylooctaose, xylononaose and xylodecaose) is detected to be 0.65kg by a high-efficiency anion exchange chromatography, and the yield is 27%.
Example 8
Crushing 10kg of bagasse (with the xylan content of 24%) to 0.5-3mm, uniformly mixing and stirring with 1.5% of aspartic acid solution according to the mass ratio of 1:2, then sending into a reactor of a steam explosion machine, introducing supersaturated steam, heating to 190 ℃, maintaining the pressure at 1.3MPa for 7min, instantaneously releasing the pressure, blasting wet materials into a receiver, and collecting a solid-liquid mixture subjected to steam explosion treatment;
separating the solid-liquid mixture by a plate-and-frame filter press, and respectively collecting liquid and solid parts; mixing the solid part with 50kg of water uniformly, then carrying out plate-frame filter pressing separation again, collecting liquid, and mixing the liquid obtained by the two solid-liquid separation to obtain xylooligosaccharide liquid.
The quality of the total xylooligosaccharide (xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose, xyloheptaose, xylooctaose, xylononaose and xylodecaose) is detected to be 0.77kg by a high-efficiency anion exchange chromatography, and the yield is 32%.
Example 9
Crushing 10kg of bagasse (with the xylan content of 24%) to a size of 0.5-3mm, uniformly mixing and stirring with an aspartic acid solution with the concentration of 0.5% according to a mass ratio of 1:2, then sending into a reactor of a steam explosion machine, introducing supersaturated steam, heating to 150 ℃, maintaining the pressure at 0.6MPa for 15min, instantaneously releasing the pressure, blasting wet materials into a receiver, and collecting a solid-liquid mixture subjected to steam explosion treatment;
separating the solid-liquid mixture by a plate-and-frame filter press, and respectively collecting liquid and solid parts;
mixing the solid part with 50kg of water uniformly, then carrying out plate-frame filter pressing separation again, collecting liquid, and mixing the liquid obtained by the two solid-liquid separation to obtain xylooligosaccharide liquid.
The quality of the total xylooligosaccharide (xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose, xyloheptaose, xylooctaose, xylononaose and xylodecaose) is detected to be 0.86kg by a high-efficiency anion exchange chromatography, and the yield is 36%.
Example 10
Crushing 10kg of bagasse (with the xylan content of 24%) to a size of 0.5-3mm, uniformly mixing and stirring with an aspartic acid solution with the concentration of 0.5% according to the mass ratio of 1:0.5, feeding into a reactor of a steam explosion machine, introducing supersaturated steam, heating to 200 ℃, maintaining the pressure for 7min, instantaneously releasing the pressure, blasting wet materials into a receiver, and collecting a solid-liquid mixture subjected to steam explosion treatment;
separating the solid-liquid mixture by a plate-and-frame filter press, and respectively collecting liquid and solid parts;
mixing the solid part with 50kg of water uniformly, then carrying out plate-frame filter pressing separation again, collecting liquid, and mixing the liquid obtained by the two solid-liquid separation to obtain xylooligosaccharide liquid.
The quality of the total xylooligosaccharide (xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose, xyloheptaose, xylooctaose, xylononaose and xylodecaose) is detected to be 0.7kg by a high-efficiency anion exchange chromatography, and the yield is 29%.
It should be noted that the above embodiments are only for illustrating the technical solution of the present application and not for limiting the same, and although the present application has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present application may be modified or substituted without departing from the spirit and scope of the technical solution of the present application, and it should be covered in the scope of the present application.
Claims (10)
1. A method for producing xylo-oligosaccharide by aspartic acid-assisted steam explosion treatment is characterized by comprising the following steps of: comprising the steps of (a) a step of,
crushing the raw material rich in xylan to obtain a crushed material;
mixing the crushed material with an aspartic acid solution, and uniformly stirring to obtain an aspartic acid-containing material;
heating the material containing aspartic acid in a steam explosion reactor by steam, and instantly releasing pressure to obtain a solid-liquid mixture;
and carrying out solid-liquid separation on the solid-liquid mixture to obtain the xylooligosaccharide liquid.
2. The method for producing xylo-oligosaccharide by aspartic acid-assisted steam explosion treatment according to claim 1, wherein the method comprises the following steps: also included is a method of manufacturing a semiconductor device,
and (3) adding distilled water into the solid obtained by solid-liquid separation of the solid-liquid mixture, uniformly stirring, and carrying out solid-liquid separation again to obtain the xylooligosaccharide liquid.
3. The method for producing xylo-oligosaccharide by aspartic acid-assisted steam explosion treatment according to claim 1 or 2, wherein the method comprises the following steps of: the xylan-rich raw material comprises corncob and bagasse.
4. The method for producing xylo-oligosaccharide by aspartic acid-assisted steam explosion treatment according to claim 3, wherein the method comprises the following steps of: the particle size of the crushed material is 0.1-10 mm.
5. The method for producing xylo-oligosaccharide by aspartic acid-assisted steam explosion treatment according to claim 4, wherein the method comprises the following steps of: the mass fraction of aspartic acid in the aspartic acid solution is 0.1-2.0%.
6. The method for producing xylo-oligosaccharide by aspartic acid-assisted steam explosion treatment according to any one of claims 1, 2, 4 or 5, wherein the method comprises the following steps: the mass ratio of the crushed material to the aspartic acid solution is 1:0.5-3.5.
7. The method for producing xylo-oligosaccharide by aspartic acid-assisted steam explosion treatment according to claim 6, wherein the method comprises the following steps: the reaction temperature of the steam explosion is 160-200 ℃, the reaction pressure is 0.6-2.0 MPa, and the reaction time is 3-20 min.
8. The method for producing xylo-oligosaccharide by aspartic acid-assisted steam explosion treatment according to claim 1, wherein the method comprises the following steps: the solid-liquid separation comprises plate-frame filter pressing and extrusion filtering.
9. The method for producing xylo-oligosaccharide by aspartic acid-assisted steam explosion treatment according to claim 2, wherein the method comprises the following steps: and adding distilled water into the solid obtained by solid-liquid separation of the solid-liquid mixture, wherein the mass of the distilled water is 3-10 times of that of the solid material.
10. The method for producing xylo-oligosaccharide by aspartic acid-assisted steam explosion treatment according to claim 2, wherein the method comprises the following steps: the solid-liquid separation comprises plate-frame filter pressing and extrusion filtering.
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| US20080248161A1 (en) * | 2005-05-16 | 2008-10-09 | Naoshi Inafuku | Method for Producing Blasting Fermentation-Treated Bagasse |
| CN110616237A (en) * | 2019-10-22 | 2019-12-27 | 天津科技大学 | Method for preparing xylo-oligosaccharide from steam-exploded plant fiber raw material |
| CN114836584A (en) * | 2022-05-23 | 2022-08-02 | 南京林业大学 | Method for producing xylooligosaccharide with assistance of amino acid |
| CN115232844A (en) * | 2022-07-08 | 2022-10-25 | 南京林业大学 | Method for preparing xylo-oligosaccharide from wood fiber raw material |
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|---|---|---|---|---|
| US20080248161A1 (en) * | 2005-05-16 | 2008-10-09 | Naoshi Inafuku | Method for Producing Blasting Fermentation-Treated Bagasse |
| CN110616237A (en) * | 2019-10-22 | 2019-12-27 | 天津科技大学 | Method for preparing xylo-oligosaccharide from steam-exploded plant fiber raw material |
| CN114836584A (en) * | 2022-05-23 | 2022-08-02 | 南京林业大学 | Method for producing xylooligosaccharide with assistance of amino acid |
| CN115232844A (en) * | 2022-07-08 | 2022-10-25 | 南京林业大学 | Method for preparing xylo-oligosaccharide from wood fiber raw material |
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