CN106636256B - Xylo-oligosaccharide with low polymerization degree and preparation method and application thereof - Google Patents
Xylo-oligosaccharide with low polymerization degree and preparation method and application thereof Download PDFInfo
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- CN106636256B CN106636256B CN201611237155.2A CN201611237155A CN106636256B CN 106636256 B CN106636256 B CN 106636256B CN 201611237155 A CN201611237155 A CN 201611237155A CN 106636256 B CN106636256 B CN 106636256B
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- xylooligosaccharide
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- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 title claims abstract description 77
- 238000006116 polymerization reaction Methods 0.000 title claims abstract description 58
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 62
- 239000007788 liquid Substances 0.000 claims abstract description 42
- 230000008569 process Effects 0.000 claims abstract description 22
- 230000035755 proliferation Effects 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 230000000694 effects Effects 0.000 claims abstract description 19
- 239000002994 raw material Substances 0.000 claims abstract description 19
- 239000007790 solid phase Substances 0.000 claims abstract description 18
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims abstract description 17
- 238000004880 explosion Methods 0.000 claims abstract description 14
- 238000001914 filtration Methods 0.000 claims abstract description 14
- 239000003513 alkali Substances 0.000 claims abstract description 12
- 244000199866 Lactobacillus casei Species 0.000 claims abstract description 9
- 235000013958 Lactobacillus casei Nutrition 0.000 claims abstract description 9
- 229940017800 lactobacillus casei Drugs 0.000 claims abstract description 9
- 241001134770 Bifidobacterium animalis Species 0.000 claims abstract description 8
- 108090000790 Enzymes Proteins 0.000 claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims abstract description 8
- 229940118852 bifidobacterium animalis Drugs 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 8
- 230000009849 deactivation Effects 0.000 claims abstract description 6
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 238000000746 purification Methods 0.000 claims abstract description 4
- 238000001035 drying Methods 0.000 claims abstract description 3
- 230000002255 enzymatic effect Effects 0.000 claims abstract description 3
- 239000000413 hydrolysate Substances 0.000 claims abstract description 3
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 32
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 31
- 150000004823 xylans Chemical class 0.000 claims description 21
- 229920001221 xylan Polymers 0.000 claims description 20
- 238000000909 electrodialysis Methods 0.000 claims description 18
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 17
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 17
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 17
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 239000012528 membrane Substances 0.000 claims description 13
- 238000004042 decolorization Methods 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 12
- 239000002029 lignocellulosic biomass Substances 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 229920000742 Cotton Polymers 0.000 claims description 7
- 239000003011 anion exchange membrane Substances 0.000 claims description 5
- 238000005341 cation exchange Methods 0.000 claims description 5
- 238000010612 desalination reaction Methods 0.000 claims description 5
- 229920002488 Hemicellulose Polymers 0.000 claims description 4
- 230000000593 degrading effect Effects 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 238000004458 analytical method Methods 0.000 claims description 2
- 108010038658 exo-1,4-beta-D-xylosidase Proteins 0.000 claims description 2
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 2
- 239000012071 phase Substances 0.000 claims description 2
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 2
- 239000012498 ultrapure water Substances 0.000 claims description 2
- 239000000047 product Substances 0.000 abstract description 17
- 230000015556 catabolic process Effects 0.000 abstract description 8
- 238000006731 degradation reaction Methods 0.000 abstract description 8
- 238000002474 experimental method Methods 0.000 abstract description 6
- LGQKSQQRKHFMLI-SJYYZXOBSA-N (2s,3r,4s,5r)-2-[(3r,4r,5r,6r)-4,5,6-trihydroxyoxan-3-yl]oxyoxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)OC1 LGQKSQQRKHFMLI-SJYYZXOBSA-N 0.000 abstract description 4
- LGQKSQQRKHFMLI-UHFFFAOYSA-N 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(O)OC1 LGQKSQQRKHFMLI-UHFFFAOYSA-N 0.000 abstract description 4
- SQNRKWHRVIAKLP-UHFFFAOYSA-N D-xylobiose Natural products O=CC(O)C(O)C(CO)OC1OCC(O)C(O)C1O SQNRKWHRVIAKLP-UHFFFAOYSA-N 0.000 abstract description 4
- JCSJTDYCNQHPRJ-UHFFFAOYSA-N 20-hydroxyecdysone 2,3-acetonide Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(OC2C(C(O)C(O)OC2)O)OC1 JCSJTDYCNQHPRJ-UHFFFAOYSA-N 0.000 abstract description 3
- 239000002028 Biomass Substances 0.000 abstract description 3
- JVZHSOSUTPAVII-UHFFFAOYSA-N Xylotetraose Natural products OCC(OC1OCC(OC2OCC(OC3OCC(O)C(O)C3O)C(O)C2O)C(O)C1O)C(O)C(O)C=O JVZHSOSUTPAVII-UHFFFAOYSA-N 0.000 abstract description 3
- JCSJTDYCNQHPRJ-FDVJSPBESA-N beta-D-Xylp-(1->4)-beta-D-Xylp-(1->4)-D-Xylp Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)C(O)OC2)O)OC1 JCSJTDYCNQHPRJ-FDVJSPBESA-N 0.000 abstract description 3
- KPTPSLHFVHXOBZ-BIKCPUHGSA-N xylotetraose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O[C@H]3[C@@H]([C@@H](O)C(O)OC3)O)OC2)O)OC1 KPTPSLHFVHXOBZ-BIKCPUHGSA-N 0.000 abstract description 3
- ABKNGTPZXRUSOI-UHFFFAOYSA-N xylotriose Natural products OCC(OC1OCC(OC2OCC(O)C(O)C2O)C(O)C1O)C(O)C(O)C=O ABKNGTPZXRUSOI-UHFFFAOYSA-N 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 2
- 239000002253 acid Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 description 12
- 239000000243 solution Substances 0.000 description 8
- 239000003377 acid catalyst Substances 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 7
- 238000000926 separation method Methods 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 229920001542 oligosaccharide Polymers 0.000 description 6
- 241000186000 Bifidobacterium Species 0.000 description 5
- 235000011054 acetic acid Nutrition 0.000 description 5
- 229910052799 carbon Inorganic materials 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 241000209140 Triticum Species 0.000 description 3
- 235000021307 Triticum Nutrition 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000010411 cooking Methods 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 238000010025 steaming Methods 0.000 description 3
- 239000010902 straw Substances 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 241000499912 Trichoderma reesei Species 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 238000013375 chromatographic separation Methods 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 238000006384 oligomerization reaction Methods 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 238000004879 turbidimetry Methods 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 238000005084 2D-nuclear magnetic resonance Methods 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 241000609240 Ambelania acida Species 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000186018 Bifidobacterium adolescentis Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108010001817 Endo-1,4-beta Xylanases Proteins 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- FTTUBRHJNAGMKL-UHFFFAOYSA-N Xylohexaose Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(OC4C(C(O)C(OC5C(C(O)C(O)OC5)O)OC4)O)OC3)O)OC2)O)OC1 FTTUBRHJNAGMKL-UHFFFAOYSA-N 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 125000000089 arabinosyl group Chemical group C1([C@@H](O)[C@H](O)[C@H](O)CO1)* 0.000 description 1
- 239000010905 bagasse Substances 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 108010089934 carbohydrase Proteins 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000012501 chromatography medium Substances 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 238000001728 nano-filtration Methods 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- ODZPKZBBUMBTMG-UHFFFAOYSA-N sodium amide Chemical compound [NH2-].[Na+] ODZPKZBBUMBTMG-UHFFFAOYSA-N 0.000 description 1
- 239000010907 stover Substances 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
The invention discloses a preparation method of xylo-oligosaccharide with low polymerization degree, which is characterized by comprising the following steps: (1) The method comprises the steps of taking lignocellulose biomass as a raw material, performing dilute acid/dilute alkali/hot water pretreatment, filtering liquid, and cooling a solid phase by adopting a high-temperature degradation or steam explosion pretreatment mode for standby; (2) Adding xylanase into the pretreatment liquid in the step (1) to carry out enzymolysis reaction; (3) The enzymatic hydrolysate is subjected to enzyme deactivation, purification, concentration and drying treatment to prepare the xylooligosaccharide with low polymerization degree. According to the invention, through reasonably optimizing each process step, the obtained xylooligosaccharide has low polymerization degree, and the xylobiose, the xylotriose and the xylotetraose are used as main substances, so that the functionality of the xylooligosaccharide serving as a bifidus factor is enhanced, and experiments prove that the prepared xylooligosaccharide has good proliferation effect on bifidobacterium animalis and lactobacillus casei. The invention simplifies the process, improves the product quality and has good application prospect.
Description
Technical Field
The invention belongs to the technical field of functional sugar alcohol production, and particularly relates to low-polymerization-degree xylooligosaccharide, a preparation method and application thereof.
Background
Xylo-oligosaccharide is formed by hydrolyzing beta-1, 4 glycosidic bonds by beta-1, 4 endoxylanase. The xylooligosaccharide can be used as a bifidus factor to multiply and increase beneficial bacteria bifidobacteria in human intestinal tracts, improve the balance of digestive tract flora of organisms (human and animals), promote the growth of beneficial bacteria in the digestive tract, inhibit the reproduction of harmful microorganisms, promote nutrient absorption and improve the immunity of organisms.
The xylobiose in the xylooligosaccharide is a carbohydrate which can not be digested and absorbed by human bodies but can be fermented, has the proliferation effect of bifidobacteria, is formed by connecting three xylose molecules through beta-1, 4 glycosidic bonds, is a carbohydrate which can not be digested and absorbed by human bodies but can be fermented, has the proliferation effect of bifidobacteria, and has similar functions as xylobiose. Bo Xiaoli, yu Shiyuan, etc. (chromatographic separation of the components of xylo-oligosaccharides and proliferation of bifidobacteria) found: the proliferation efficiency of the xylo-oligosaccharide with the polymerization degree of 2-5 on bifidobacterium adolescentis is obviously stronger than that of the xylo-oligosaccharide with the polymerization degree of 6-8. It can be seen that the low degree of polymerization XOS 2-4 Is the main active ingredient in the xylooligosaccharide product, and the content of the active ingredient plays a key role in the proliferation of bifidus factors. In addition, the proportion content of the low-polymerization-degree xylo-oligosaccharide is improved, and the method can be achieved indirectly through removing the high-polymerization-degree xylo-oligosaccharide.
The existing xylooligosaccharide products mainly comprise xylan with the polymerization degree of 2-9, the xylan with the polymerization degree of 7-9 in the products has larger proportion content, and the purity of xylobiose, xylotriose and xylotetraose in the products is only about 60%, so that the proliferation efficiency of bifidus factors of the products is greatly reduced. In the current patent and literature data, the preparation of the xylo-oligosaccharide with low polymerization degree is mainly prepared by adopting techniques of medium separation elution such as polyacrylamide gel, active carbon and the like, combination of enzyme biocatalysis and membrane separation and the like: the patent CN 1847254A adopts polyacrylamide gel as a chromatographic medium for elution and separation to prepare the xylo-oligosaccharide with various polymerization degrees; patent CN101632877A describes a method for preparing various degrees of polymerization of xylo-oligosaccharide by separating and purifying each single component of xylo-oligosaccharide by using activated carbon; the 102796783A patent adopts the technology of combining the biocatalysis of multifunctional carbohydrase and membrane separation to prepare the functional glucan product with the polymerization degree of 3-8. The preparation methods have complicated separation and differentiation steps and are not suitable for industrial production and application. The patent CN1364911A generates xylanase with high activity by controlling the reaction conditions of the pre-enzyme production stage and the post-enzyme production stage of Trichoderma reesei, and utilizes the xylanase to hydrolyze xylan to prepare xylooligosaccharide with the polymerization degree of 2-5. However, the initial raw material in the preparation method is xylan, and the xylan is purified by methods of ethanol precipitation, ultrafiltration, nanofiltration column chromatography and the like, so that the application range of the technology is limited.
Disclosure of Invention
In order to make up the defects of the prior art, the invention provides a preparation method of xylo-oligosaccharide with low polymerization degree. The invention adopts pretreatment technology and enzymolysis technology, combines electrodialysis method to purify xylooligosaccharide liquid, can reduce acid-base loss in the resin treatment process, and can recycle residual liquid after electrodialysis to prepare xylooligosaccharide with low polymerization degree, which has beneficial proliferation to functional bifidus factor, simple production technology and high production efficiency, thereby not only improving the quality of the xylooligosaccharide, but also improving the production efficiency.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
in a first aspect of the invention, a method for preparing xylo-oligosaccharide with low polymerization degree is provided, comprising the following steps:
(1) The method comprises the steps of taking lignocellulose biomass as a raw material, performing dilute alkali/hot water pretreatment, filtering liquid, degrading hemicellulose long chains in the lignocellulose raw material by adopting a high-temperature degradation or steam explosion pretreatment mode on a solid phase, dissolving out xylan in the lignocellulose raw material, and controlling the xylose content in the pretreated feed liquid to be 5-20%; cooling for standby;
(2) Adding xylanase accounting for 0.01-0.03% of the dry weight of the lignocellulose biomass into the pretreatment liquid in the step (1) for enzymolysis reaction;
(3) The enzymatic hydrolysate is subjected to enzyme deactivation, purification, concentration and drying treatment to prepare the xylooligosaccharide with low polymerization degree.
Preferably, the lignocellulosic biomass in step (1) comprises: one or more of cotton seed hulls, corn cobs, corn stover, bagasse, or wheat bran; further preferably the lignocellulosic biomass is corncob;
preferably, the pretreatment method of the lignocellulosic biomass raw material in the step (1) specifically comprises the following steps:
hot water method: mixing the lignocellulosic biomass raw material and process water according to the mass ratio of 1 (6-12), performing hot water treatment at 50-100 ℃ for 30-90 min, and filtering liquid;
dilute alkaline process: mixing the lignocellulosic biomass raw material with process water according to the mass ratio of 1 (6-12), adding an alkali solution with the dry weight percentage of 0.01-0.5% of the lignocellulosic biomass raw material, carrying out hot water treatment for 30-90 min at 50-100 ℃, filtering the liquid, and washing until the solid phase is close to pH 7; wherein the alkali is one or more of sodium hydroxide, potassium hydroxide, ammonia water or sodium amide;
the pretreatment mode also comprises other pretreatment processes with the same effect as the pretreatment mode;
preferably, the method for performing high-temperature degradation on the solid phase in the step (1) specifically comprises the following steps: mixing the solid phase and the process water according to the mass ratio of 1 (6-12), adding a weak acid catalyst with the solid phase dry weight percentage of 0.3-1.5%, and steaming at 160-170 ℃ for 30-90 min;
preferably, in the step (1), the steam explosion method for the solid phase specifically includes: mixing the solid phase and the process water according to the mass ratio of 1 (1-5), adding a weak acid catalyst with the solid phase dry weight percentage of 0.3-1.2%, and treating the mixture under the pressure of 1.5-2.5MPa for 30s-300s;
the weak acid catalyst in the high-temperature degradation method and the steam explosion method is one or more of acetic acid, formic acid, citric acid, lactic acid, sulfurous acid or carbonic acid;
the pretreatment mode also comprises other pretreatment degradation methods with the same effect as the pretreatment degradation method;
further preferably, the method for calculating the xylose content in the feed liquid comprises the following steps: HPLC detection, wherein the peak area of the xylose component accounts for the percentage of the total sugar peak area; the detection conditions are as follows: the chromatographic column is Shodex sugamer KS-802 or other chromatographic columns with the same analysis effect; the mobile phase is ultrapure water, the column temperature is 80 ℃, and the flow rate is 0.5-0.7ml/min;
preferably, the specific steps in the step (1) are as follows:
the method comprises the steps of taking cotton seed hulls as raw materials, performing pretreatment by a dilute alkali method, filtering liquid, degrading hemicellulose long chains in a lignocellulose raw material by adopting a steam explosion pretreatment mode on a solid phase, dissolving out xylan in the lignocellulose raw material, controlling the xylose content in the pretreated feed liquid to be 5-20%, and cooling for later use;
wherein, the dilute alkali method is to mix cotton seed hulls with process water according to the mass ratio of 1 (6-12), add alkaline solution with the dry weight percentage of 0.01-0.5% of the cotton seed hulls raw materials, heat water at 50-100 ℃ for 30min-90min, filter the liquid, and wash the liquid until the solid phase approaches pH to 7; wherein the base is sodium hydroxide;
the specific method for the steam explosion pretreatment comprises the following steps: mixing the solid phase and the process water according to the mass ratio of 1 (1-5), adding a weak acid catalyst with the solid phase dry weight percentage of 0.3-1.2%, and treating at the treatment pressure of 1.5-2.5MPa and the treatment temperature of 150-190 ℃ for 30s-300s; wherein the weak acid catalyst is acetic acid;
the invention adopts the dilute alkali method combined with the steam explosion method to pretreat the xylooligosaccharide, and the inventor finds that the dilute alkali method can effectively remove acetyl, glucosyl and arabinosyl on a xylan main chain in the raw material, so that the monosaccharide content of the product is greatly reduced, and the invention is beneficial to improving the proportion of xylan with low polymerization degree in the product; meanwhile, the pH value of the solution after adding alkali is maintained between 8 and 10 by the adding proportion, so that the reduction of the yield of xylooligosaccharide caused by the destruction of a xylan structure due to the over high alkalinity is effectively avoided; meanwhile, the effect that the mixed sugar chain, acetyl and a small amount of lignin and colloid cannot be removed due to too low alkalinity is avoided; and then further mechanical destruction is carried out on plant cell walls by a steam explosion method, so that xylan is further dissolved in the extracting solution, and meanwhile, as the dilute alkali method treatment is carried out in the early stage and the weak acid catalyst is added in the steam explosion method, the reaction temperature and pressure of the steam explosion method are effectively reduced, and the color of the extracting solution is prevented from being darker;
meanwhile, the inventor unexpectedly discovers that the index of the xylose content after pretreatment is very important for controlling the degradation degree and the dissolution effect of xylan in practical research; when the xylose content is too high (more than 20%), although the dissociation degree of xylan is improved, the byproducts are obviously increased, so that the content proportion of xylooligosaccharide obtained in the subsequent enzymolysis process is reduced, and when the pretreated xylose content is lower (less than 5%), the dissociation degree of xylan is lower, the enzymolysis of the subsequent xylanase is not facilitated, and the subsequent xylooligosaccharide yield is also reduced; and after pretreatment and pretreatment processes, the subsequent enzymolysis time is reduced, and the xylanase consumption is reduced, thereby being beneficial to industrial production.
Preferably, the xylanase in the step (2) is beta-1, 4-endo xylanase prepared by fermenting Trichoderma reesei, aspergillus viride or other strains with equivalent effects by microorganisms, and the xylanase has the auxiliary effect of degrading hemicellulose, such as beta-xylosidase, alpha-L-arabinosidase and the like;
preferably, the purifying step in the step (3) comprises activated carbon decolorization and electrodialysis desalination, wherein the decolorization temperature of the activated carbon is 50-90 ℃, the addition amount of the activated carbon is 0.1-2%, the decolorization time is 20-90min, and the plate frame filtration is carried out after the decolorization is completed; the conductivity of the electrodialysis desalted standard feed liquid is less than or equal to 800 mu s/cm, the pH value is 4-8, the electrodialysis temperature is 15-42 ℃, the membrane pressure difference of the cation exchange membrane and the anion exchange membrane is 0.2-0.8MPa, and the residual liquid in the anode chamber and the residual liquid in the cathode chamber are recycled.
In a second aspect of the invention, the xylo-oligosaccharide with low polymerization degree prepared by the preparation method is disclosed. The xylo-oligosaccharide obtained by the preparation method has the polymerization degree (Degree of Polymerization, DP for short) of 2-6, the purity of the xylo-oligosaccharide is more than or equal to 75 percent, wherein the purity of the xylo-oligosaccharide with the polymerization degree of 2-4 is more than or equal to 70 percent, and meanwhile, the xylo-oligosaccharide does not contain arabinose and acetyl side chains and has the structural formula shown as follows: wherein n=0 to 4, a, B, C and D correspond to the 4 end groups in two-dimensional nuclear magnetic resonance spectroscopy, respectively;
in a third aspect of the invention, the application of the xylooligosaccharide with low polymerization degree in proliferation of bifidobacterium animalis and lactobacillus casei is disclosed. Because the preparation methods are different, the purity of the xylo-oligosaccharide, the ratio of the xylo-oligosaccharide with various polymerization degrees and the ratio of the components of impurities in the obtained xylo-oligosaccharide product are different, the xylo-oligosaccharide with the structure and/or composition of the invention is not provided in the prior art, and the xylo-oligosaccharide product prepared by the invention has good effect on the proliferation of bifidobacterium animalis and lactobacillus casei through test verification.
The beneficial effects of the invention are as follows:
through the synergistic effect of the pretreatment and the pretreatment process, the xylose dissolution and degradation effects can be effectively improved, meanwhile, the chromaticity of the extracting solution and the production of byproducts can be effectively reduced, the xylanase addition amount can be obviously reduced, the enzymolysis reaction time is shortened, and the product purity is improved;
the invention adopts an electrodialysis method to replace the application of ion exchange resin in the preparation, reduces the acid-base consumption and resin regeneration, and saves the production cost; the preparation of the xylooligosaccharide with low polymerization degree by a one-step method reduces elution and separation steps such as polyacrylamide gel, active carbon, membrane separation, chromatographic separation and the like in the traditional preparation method, and simplifies the process.
In a word, the method provided by the invention has the advantages that through reasonably optimizing each process step, the polymerization degree of the obtained xylooligosaccharide is low, and the xylooligosaccharide, xylotriose and xylotetraose are used as main substances, so that the functionality of the xylooligosaccharide serving as a bifidus factor is enhanced, and the experiment proves that the prepared xylooligosaccharide has good proliferation effect on bifidobacterium animalis and lactobacillus casei. In a word, the invention simplifies the process, improves the product quality and has good application prospect.
Detailed Description
The present invention will be described in further detail with reference to examples, but embodiments of the present invention are not limited thereto.
Example 1
Mixing 450g of cotton seed hulls with 0.05% sodium hydroxide solution according to a mass ratio of 1:7, heating to 60 ℃, preserving heat for 30min, and filtering out liquid; washing with water for one time until the pH value is neutral; mixing the mixture with process water according to the mass ratio of 1:3, adding a weak acid catalyst (acetic acid) with the dry weight percentage of 0.7 percent of cotton seed hulls, performing steam explosion treatment, wherein the treatment pressure is 1.5-2.5MPa, and the treatment time is 120s; xylose content in the gas explosion liquid is 5.85%, and the temperature is reduced for standby. Adding 0.8g xylanase into the cooking liquor, wherein the enzymolysis reaction temperature is 60 ℃, the enzymolysis time is 8 hours, and the low polymerization degree xylo-oligosaccharide is prepared by enzyme deactivation, active carbon decolorization, electrodialysis desalination and concentration of the enzymolysis liquor. The decolorizing temperature of the activated carbon is 50-90 ℃, the adding amount of the activated carbon is 0.1-2%, the decolorizing time is 20-90min, and the plate and frame filtration is carried out after the decolorizing is completed; the conductivity of the electrodialysis desalted standard feed liquid is less than or equal to 800 mu s/cm, the pH value is 4-8, the electrodialysis temperature is 15-42 ℃, the membrane pressure difference of the cation exchange membrane and the anion exchange membrane is 0.2-0.8MPa, and the residual liquid in the anode chamber and the residual liquid in the cathode chamber are recycled.
TABLE 1 content of xylo-oligosaccharide component having low polymerization degree in example 1
As can be seen from table 1: in the product of example 1, the purity of the xylooligosaccharide with the oligomerization degree of 2-6 is 87.21%, the xylooligosaccharide with the high polymerization degree of no xyloheptaose and the like is not contained, and the purity of the xylan with the polymerization degree of 2-4 is 83.04%.
Example 2
Taking 450g of corncob, mixing with process water according to the mass ratio of 1:6, heating to 80 ℃, preserving heat for 40min, and filtering out liquid; adding acetic acid with the dry weight percentage of 0.8% into the corncob, and steaming at high temperature, wherein the treatment conditions are as follows: the stewing temperature is 165 ℃ and the stewing time is 50min, the xylan in the corncob is dissolved out, the xylose content in the stewing liquid is 15.75%, and the temperature is reduced for standby. Adding 0.1g xylanase into the cooking liquor, wherein the enzymolysis reaction temperature is 80 ℃, the enzymolysis time is 8 hours, and the low polymerization degree xylo-oligosaccharide is prepared by enzyme deactivation, active carbon decolorization, electrodialysis desalination and concentration of the enzymolysis liquor. The decolorizing temperature of the activated carbon is 50-90 ℃, the adding amount of the activated carbon is 0.1-2%, the decolorizing time is 20-90min, and the plate and frame filtration is carried out after the decolorizing is completed; the conductivity of the electrodialysis desalted standard feed liquid is less than or equal to 800 mu s/cm, the pH value is 4-8, the electrodialysis temperature is 15-42 ℃, the membrane pressure difference of the cation exchange membrane and the anion exchange membrane is 0.2-0.8MPa, and the residual liquid in the anode chamber and the residual liquid in the cathode chamber are recycled.
TABLE 2 content of xylo-oligosaccharide component having low polymerization degree in example 2
As can be seen from table 2: in the product of example 2, the purity of the low polymerization degree xylooligosaccharide is 79.16%, the high polymerization degree xylooligosaccharide such as xyloheptase and the like is not contained, and the purity of the polymerization degree 2-4 xylan is 71.18%.
Example 3
450g of wheat straw is taken and mixed with process water according to the mass ratio of 1:10, the temperature is raised to 90 ℃, the heat is preserved for 60min, and liquid is filtered; mixing the mixture with process water according to the mass ratio of 1:8, adding acetic acid with the dry weight percentage of 1.2% of wheat straw, and carrying out high-temperature steaming treatment under the following conditions: the stewing temperature is 165 ℃, the stewing time is 60 minutes, the xylan in the wheat straw is dissolved out, the xylose content in the stewing liquid is 19.96%, and the temperature is reduced for standby. Adding 0.4g xylanase into the cooking liquor, wherein the enzymolysis reaction temperature is 80 ℃, the enzymolysis time is 8 hours, and the low polymerization degree xylo-oligosaccharide is prepared by enzyme deactivation, active carbon decolorization, electrodialysis desalination and concentration of the enzymolysis liquor. The decolorizing temperature of the activated carbon is 50-90 ℃, the adding amount of the activated carbon is 0.1-2%, the decolorizing time is 20-90min, and the plate and frame filtration is carried out after the decolorizing is completed; the conductivity of the electrodialysis desalted standard feed liquid is less than or equal to 800 mu s/cm, the pH value is 4-8, the electrodialysis temperature is 15-42 ℃, the membrane pressure difference of the cation exchange membrane and the anion exchange membrane is 0.2-0.8MPa, and the residual liquid in the anode chamber and the residual liquid in the cathode chamber are recycled.
TABLE 3 content of xylo-oligosaccharide component having low polymerization degree in example 3
As can be seen from table 3: in the product of example 3, the purity of the xylooligosaccharide with low polymerization degree is 75.09%, the xylooligosaccharide with high polymerization degree such as xyloheptaose, xylohexaose and the like is not contained, and the purity of the xylan with polymerization degree of 2-4 is 72.11%.
Comparative example 1
The method is the same as in example 1, the only difference is that the treatment step of the dilute alkali method is omitted, namely, the mixing of sodium hydroxide solution with the concentration of 0.05% according to the mass ratio of 1:7 is omitted, the temperature is raised to 60 ℃, the temperature is kept for 30min, and the liquid is filtered; washing with water once until the pH value is neutral.
TABLE 4 content of xylo-oligosaccharide component having low polymerization degree in comparative example 1
As can be seen from table 4: in the product of comparative example 1, the purity of xylooligosaccharide with the oligomerization degree of 2-6 is 77.79 percent, xyloheptasaccharide is contained, and the purity of xylan with the polymerization degree of 2-4 is 73.51 percent.
Example 4
In vitro anaerobic culture experiments for Lactobacillus casei proliferation were performed on the low polymerization degree xylo-oligosaccharides obtained in examples 1-3 and comparative example 1: taking MRS culture medium with glucose removed as basic culture medium, adding different xylo-oligosaccharides to replace glucose as xylo-oligosaccharide culture medium. The basic culture medium is used as a control, the turbidimetry is adopted to measure the growth quantity, the OD600nm is measured at different culture time, and a growth curve is drawn.
Activating strains: inoculating lactobacillus glycerinum strain preserved at-85deg.C into MRS liquid culture medium with an inoculum size of 1%, and culturing at 36deg.C for 36 hr.
Lactobacillus casei proliferation: adding sterilized syrup and glucose into corresponding liquid culture mediums, inoculating activated lactobacillus casei into each culture medium, and culturing at 36 deg.C for 36 hr.
As can be seen from in vitro proliferation experiments, the proliferation effect of the xylo-oligosaccharide with low polymerization degree on lactobacillus casei is better than that of the conventional xylo-oligosaccharide, and the proliferation effect of the examples 1-3 is better than that of the comparative example 1.
Example 5
In vitro anaerobic culture experiments for proliferation of bifidobacterium animalis were performed on the low degree of polymerization xylo-oligosaccharides obtained in examples 1-3 and comparative example 1: taking MRS culture medium with glucose removed as basic culture medium, adding different xylo-oligosaccharides to replace glucose as xylo-oligosaccharide culture medium. The basic culture medium is used as a control, the turbidimetry is adopted to measure the growth quantity, the OD600nm is measured at different culture time, and a growth curve is drawn.
Activating strains: inoculating glycerol freezing tube strain preserved at-85deg.C into basic culture medium with an inoculum size of 1%, and culturing at 36deg.C for 36 hr.
Proliferation of bifidobacterium animalis: adding sterilized oligosaccharide and glucose into corresponding liquid culture mediums, inoculating activated animal bifidobacterium into each culture medium, and culturing at 36 ℃ for 48h with an inoculum size of 1%.
As can be seen from in vitro proliferation experiments, the proliferation effect of the xylooligosaccharide with low polymerization degree on bifidobacterium animalis is better than that of the conventional xylooligosaccharide, and the proliferation effect of the examples 1-3 is better than that of the comparative example 1.
The foregoing examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the foregoing examples, and it should be understood that, based on the technical solution of the present invention, various modifications or changes may be made by those skilled in the art without any inventive effort, and still remain within the scope of the present invention.
Claims (6)
1. The preparation method of the xylo-oligosaccharide with low polymerization degree is characterized by comprising the following steps:
(1) The method comprises the steps of taking cotton seed hulls as raw materials, performing pretreatment by a dilute alkali method, filtering liquid, degrading hemicellulose long chains in a lignocellulose raw material by adopting a steam explosion pretreatment mode on a solid phase, dissolving out xylan in the lignocellulose raw material, controlling the xylose content in the pretreated feed liquid to be 5-20%, and cooling for later use;
(2) Adding xylanase into the pretreatment liquid in the step (1) to carry out enzymolysis reaction;
(3) The enzymatic hydrolysate is subjected to enzyme deactivation, purification, concentration and drying treatment to prepare the xylooligosaccharide with low polymerization degree;
dilute alkaline process: mixing the lignocellulosic biomass raw material with process water according to the mass ratio of 1 (6-12), adding sodium hydroxide with the dry weight percentage of 0.05% of the lignocellulosic biomass raw material, treating with hot water at 50-100 ℃ for 30-90 min, filtering liquid, and washing until the solid phase is close to pH 7;
the method for performing steam explosion on the solid phase in the step (1) specifically comprises the following steps: mixing the solid phase and the process water according to the mass ratio of 1 (1-5), adding acetic acid with the solid phase dry weight percentage of 0.7%, and treating at the pressure of 1.5-2.5MPa and the treatment temperature of 150-190 ℃ for 30s-300s.
2. The method for producing an oligoxylose of low degree of polymerization according to claim 1, wherein the xylose content in the feed liquid after pretreatment in the step (1) is 5 to 20%;
the method for calculating the xylose content in the feed liquid comprises the following steps: HPLC detection, wherein the peak area of the xylose component accounts for the percentage of the total sugar peak area; the detection conditions are as follows: the chromatographic column is Shodex sugamer KS-802 or other chromatographic columns with the same analysis effect; the mobile phase is ultrapure water, the column temperature is 80 ℃, and the flow rate is 0.5-0.7ml/min.
3. The method for preparing xylooligosaccharide with low degree of polymerization according to claim 1, wherein the xylanase in the step (2) comprises beta-1, 4-endo xylanase, beta-xylosidase and alpha-L-arabinosidase, and the addition amount of the xylanase is 0.01-0.03% of the dry weight of the lignocellulosic biomass.
4. The method for preparing xylooligosaccharide with low degree of polymerization according to claim 1, wherein the purification step in the step (3) comprises activated carbon decolorization and electrodialysis desalination, wherein the decolorization temperature adopted by the activated carbon decolorization is 50-90 ℃, the addition amount of the activated carbon is 0.1-2%, the decolorization time is 20-90min, and the plate and frame filtration is carried out after the decolorization is completed; the conductivity of the electrodialysis desalted standard feed liquid is less than or equal to 800 mu s/cm, the pH value is 4-8, the electrodialysis temperature is 15-42 ℃, the membrane pressure difference of the cation exchange membrane and the anion exchange membrane is 0.2-0.8MPa, and the residual liquid in the anode chamber and the residual liquid in the cathode chamber are recycled.
5. The xylo-oligosaccharide with low polymerization degree prepared by the preparation method of any one of claims 1 to 4, wherein the polymerization degree of the xylo-oligosaccharide with low polymerization degree is between 2 and 6, the purity of the xylo-oligosaccharide is more than or equal to 75 percent, and the purity of the xylo-oligosaccharide with the polymerization degree of 2 to 4 is more than or equal to 70 percent.
6. The use of the xylooligosaccharide of low degree of polymerization according to claim 5 for the proliferation of bifidobacterium animalis and lactobacillus casei.
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| CN109055227B (en) * | 2018-09-27 | 2022-06-10 | 广州市金因源生物技术有限公司 | Protective agent for freeze-drying preservation of genetically engineered strain and preservation method thereof |
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| CN110004194B (en) * | 2019-04-18 | 2021-11-02 | 四川金象赛瑞化工股份有限公司 | Method for producing xylose and furfural by utilizing bagasse enzymolysis |
| CN109957982B (en) * | 2019-04-24 | 2020-11-17 | 吴学军 | Programmed explosion pulping method for crop straws |
| CN110256510B (en) * | 2019-06-06 | 2023-03-07 | 南京林业大学 | Method for preparing multi-component xylo-oligosaccharide |
| CN110973421A (en) * | 2019-11-29 | 2020-04-10 | 中国农业科学院农产品加工研究所 | High-content xylo-oligosaccharide corn bamboo shoot beverage and preparation method thereof |
| CN111004827B (en) * | 2019-12-27 | 2021-09-03 | 山东百龙创园生物科技股份有限公司 | Preparation method of xylo-oligosaccharide |
| CN111575328A (en) * | 2020-05-22 | 2020-08-25 | 南京林业大学 | Method for preparing xylo-oligosaccharide by coupling acid hydrolysis and enzyme hydrolysis |
| CN113151614A (en) * | 2021-03-17 | 2021-07-23 | 南京林业大学 | Method for preparing xylooligosaccharide from agricultural and forestry waste through two-step acetic acid hydrolysis |
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