CN106636256A - Xylooligosaccharide with low DP (degree of polymerization) as well as preparation method and application of xylooligosaccharide - Google Patents
Xylooligosaccharide with low DP (degree of polymerization) as well as preparation method and application of xylooligosaccharide Download PDFInfo
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- CN106636256A CN106636256A CN201611237155.2A CN201611237155A CN106636256A CN 106636256 A CN106636256 A CN 106636256A CN 201611237155 A CN201611237155 A CN 201611237155A CN 106636256 A CN106636256 A CN 106636256A
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- solid phase
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- xylose
- oligomeric xylose
- xylooligosaccharide
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Links
- 238000006116 polymerization reaction Methods 0.000 title claims abstract description 59
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 title abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 44
- 239000007788 liquid Substances 0.000 claims abstract description 35
- 239000007790 solid phase Substances 0.000 claims abstract description 24
- 229920002522 Wood fibre Polymers 0.000 claims abstract description 19
- 239000002994 raw material Substances 0.000 claims abstract description 18
- 239000002028 Biomass Substances 0.000 claims abstract description 17
- 239000002025 wood fiber Substances 0.000 claims abstract description 16
- 238000004880 explosion Methods 0.000 claims abstract description 15
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims abstract description 13
- 230000008569 process Effects 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 claims abstract description 10
- 102000004190 Enzymes Human genes 0.000 claims abstract description 10
- 244000199866 Lactobacillus casei Species 0.000 claims abstract description 8
- 235000013958 Lactobacillus casei Nutrition 0.000 claims abstract description 8
- 229940017800 lactobacillus casei Drugs 0.000 claims abstract description 8
- 239000002253 acid Substances 0.000 claims abstract description 4
- 238000000746 purification Methods 0.000 claims abstract description 4
- 238000012360 testing method Methods 0.000 claims abstract description 3
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 160
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 82
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 81
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 32
- 239000013505 freshwater Substances 0.000 claims description 32
- 150000004823 xylans Chemical class 0.000 claims description 23
- 229920001221 xylan Polymers 0.000 claims description 22
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 21
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 230000000694 effects Effects 0.000 claims description 17
- 238000002156 mixing Methods 0.000 claims description 17
- 238000000909 electrodialysis Methods 0.000 claims description 14
- 239000003377 acid catalyst Substances 0.000 claims description 13
- 238000004061 bleaching Methods 0.000 claims description 10
- 238000010612 desalination reaction Methods 0.000 claims description 10
- 241001465754 Metazoa Species 0.000 claims description 9
- 230000015556 catabolic process Effects 0.000 claims description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- 238000006731 degradation reaction Methods 0.000 claims description 9
- 241000186000 Bifidobacterium Species 0.000 claims description 8
- 229920000742 Cotton Polymers 0.000 claims description 8
- 101100341609 Drosophila melanogaster jing gene Proteins 0.000 claims description 8
- 240000008042 Zea mays Species 0.000 claims description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 8
- 235000005822 corn Nutrition 0.000 claims description 8
- 239000002023 wood Substances 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 238000002203 pretreatment Methods 0.000 claims description 7
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- 238000004090 dissolution Methods 0.000 claims description 6
- 238000001976 enzyme digestion Methods 0.000 claims description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 6
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 239000003513 alkali Substances 0.000 claims description 5
- 239000003011 anion exchange membrane Substances 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 239000010902 straw Substances 0.000 claims description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 4
- 229920002488 Hemicellulose Polymers 0.000 claims description 4
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 238000010025 steaming Methods 0.000 claims description 4
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 3
- 235000011054 acetic acid Nutrition 0.000 claims description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 claims description 3
- 238000005341 cation exchange Methods 0.000 claims description 3
- 235000015165 citric acid Nutrition 0.000 claims description 3
- 235000019253 formic acid Nutrition 0.000 claims description 3
- 239000004310 lactic acid Substances 0.000 claims description 3
- 235000014655 lactic acid Nutrition 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 241000609240 Ambelania acida Species 0.000 claims description 2
- 229910021529 ammonia Inorganic materials 0.000 claims description 2
- 239000010905 bagasse Substances 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 238000000205 computational method Methods 0.000 claims description 2
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 2
- ODZPKZBBUMBTMG-UHFFFAOYSA-N sodium amide Chemical compound [NH2-].[Na+] ODZPKZBBUMBTMG-UHFFFAOYSA-N 0.000 claims description 2
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 2
- 239000012498 ultrapure water Substances 0.000 claims description 2
- 235000015099 wheat brans Nutrition 0.000 claims description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 claims 2
- JCSJTDYCNQHPRJ-UHFFFAOYSA-N 20-hydroxyecdysone 2,3-acetonide Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(OC2C(C(O)C(O)OC2)O)OC1 JCSJTDYCNQHPRJ-UHFFFAOYSA-N 0.000 abstract description 5
- JCSJTDYCNQHPRJ-FDVJSPBESA-N beta-D-Xylp-(1->4)-beta-D-Xylp-(1->4)-D-Xylp Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)C(O)OC2)O)OC1 JCSJTDYCNQHPRJ-FDVJSPBESA-N 0.000 abstract description 5
- ABKNGTPZXRUSOI-UHFFFAOYSA-N xylotriose Natural products OCC(OC1OCC(OC2OCC(O)C(O)C2O)C(O)C1O)C(O)C(O)C=O ABKNGTPZXRUSOI-UHFFFAOYSA-N 0.000 abstract description 5
- JVZHSOSUTPAVII-UHFFFAOYSA-N Xylotetraose Natural products OCC(OC1OCC(OC2OCC(OC3OCC(O)C(O)C3O)C(O)C2O)C(O)C1O)C(O)C(O)C=O JVZHSOSUTPAVII-UHFFFAOYSA-N 0.000 abstract description 4
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 2
- LGQKSQQRKHFMLI-SJYYZXOBSA-N (2s,3r,4s,5r)-2-[(3r,4r,5r,6r)-4,5,6-trihydroxyoxan-3-yl]oxyoxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)OC1 LGQKSQQRKHFMLI-SJYYZXOBSA-N 0.000 abstract 1
- LGQKSQQRKHFMLI-UHFFFAOYSA-N 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose Natural products OC1C(O)C(O)COC1OC1C(O)C(O)C(O)OC1 LGQKSQQRKHFMLI-UHFFFAOYSA-N 0.000 abstract 1
- 241001134770 Bifidobacterium animalis Species 0.000 abstract 1
- SQNRKWHRVIAKLP-UHFFFAOYSA-N D-xylobiose Natural products O=CC(O)C(O)C(CO)OC1OCC(O)C(O)C1O SQNRKWHRVIAKLP-UHFFFAOYSA-N 0.000 abstract 1
- 229940118852 bifidobacterium animalis Drugs 0.000 abstract 1
- 230000009849 deactivation Effects 0.000 abstract 1
- 230000000593 degrading effect Effects 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 1
- 230000002062 proliferating effect Effects 0.000 abstract 1
- KPTPSLHFVHXOBZ-BIKCPUHGSA-N xylotetraose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)CO[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O[C@H]3[C@@H]([C@@H](O)C(O)OC3)O)OC2)O)OC1 KPTPSLHFVHXOBZ-BIKCPUHGSA-N 0.000 abstract 1
- 239000000047 product Substances 0.000 description 16
- 239000001963 growth medium Substances 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 241000186016 Bifidobacterium bifidum Species 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 150000003742 xyloses Chemical class 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 239000000470 constituent Substances 0.000 description 4
- 238000010411 cooking Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 238000010792 warming Methods 0.000 description 4
- SQNRKWHRVIAKLP-RSZZQXBVSA-N (2r,3r,4r)-2,3,5-trihydroxy-4-[(2s,3r,4s,5r)-3,4,5-trihydroxyoxan-2-yl]oxypentanal Chemical compound O=C[C@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H](O)[C@H](O)[C@H]1O SQNRKWHRVIAKLP-RSZZQXBVSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 241000209140 Triticum Species 0.000 description 3
- 235000021307 Triticum Nutrition 0.000 description 3
- 239000007640 basal medium Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 238000009413 insulation Methods 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 239000011347 resin Substances 0.000 description 3
- 229920005989 resin Polymers 0.000 description 3
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 241000499912 Trichoderma reesei Species 0.000 description 2
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 2
- 238000005422 blasting Methods 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 108010089934 carbohydrase Proteins 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 150000002482 oligosaccharides Chemical class 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 238000004879 turbidimetry Methods 0.000 description 2
- -1 α-L-arabinose glycosides Chemical class 0.000 description 2
- 238000007445 Chromatographic isolation Methods 0.000 description 1
- 108010001817 Endo-1,4-beta Xylanases Proteins 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000012501 chromatography medium Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 238000001728 nano-filtration Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000005311 nuclear magnetism Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
The invention discloses a preparation method of xylooligosaccharide with low DP (degree of polymerization). The preparation method is characterized by comprising steps as follows: (1) wood fiber biomass is taken as a raw material and subjected to diluted acid/diluted base/hot water pretreatment, a liquid is filtered, a solid phase is treated in a high-temperature degrading or steam explosion pretreatment manner, and the wood fiber biomass is cooled for later use; (2) xylanase is added to a pretreatment solution in step (1) for an enzymatic hydrolysis reaction; (3) an enzymatic hydrolysis solution is treated through enzyme deactivation, purification, concentration and drying, and xylooligosaccharide with low DP is prepared. The DP of finally obtained xylooligosaccharide is low by reasonably optimizing each process step, and xylobiose, xylotriose and xylotetraose are taken as main components, so that the functionality of xylooligosaccharide as a bifidus factor is enhanced. Tests verify that prepared xylooligosaccharide has a good proliferative effect on bifidobacterium animalis and lactobacillus casei. The process is simplified, the product quality is improved, and xylooligosaccharide has good application prospect.
Description
Technical field
The invention belongs to technical field of functional sugar alcohol production, and in particular to a kind of low polymerization degree oligomeric xylose and its preparation side
Method and application.
Background technology
Oligomeric xylose is by β-Isosorbide-5-Nitrae endo-xylanase hydrolysis β-Isosorbide-5-Nitrae glucosides bond formed.Oligomeric xylose can be used as bifid
The factor makes the probioticss bacillus bifiduss in human body intestinal canal be doubled and redoubled, and improves organism (humans and animals) archenteric flora balance,
The growth of facilitating digestion road beneficial bacteria, suppresses harmful microbe breeding, promotes alimentation, improves immunity of organisms.
1,4-.beta.-Xylobiose in oligomeric xylose is can not to digest and assimilate for human body but fermentable glucide, and tool bacillus bifiduss increase
Efficiency is grown, xylotriose is that three xylose molecules are formed so that β-Isosorbide-5-Nitrae glycosidic bond is connected, is equally not digest and assimilate for human body but can send out
The glucide of ferment, it may have bacillus bifiduss breed efficiency, Xylotetrose. and xylotriose functional similarity.Bu Xiaoli, remaining generation Yuan etc. are (low
The chromatography of xylan each component and the propagation to bacillus bifiduss) research discovery：The degree of polymerization is the oligomeric xylose of 2-5 to the youth
The propagation efficiency of bacillus bifiduss is significantly stronger than the degree of polymerization for 6-8 oligomeric xyloses.It can be seen that, low polymerization degree XOS2-4It is that oligomeric xylose is produced
Principle active component in product, how many pairs of its content its bifidus factor proliferation functions play pivotal role.In addition, being lifted oligomeric
The proportional amount of right oligomeric xylose, can reach indirectly by the method for the oligomeric xylose for removing high polymerization degree.
At present oligomeric xylose product is mainly made up of the xylan of degree of polymerization 2-9, and the xylan of degree of polymerization 7-9 is accounted in product
There is larger proportional amount, 1,4-.beta.-Xylobiose, xylotriose, Xylotetrose. purity only have 60% or so in product, make the bifidus factor of product
Propagation efficiency is had a greatly reduced quality.In current patent and documents and materials, prepare low polymerization degree oligomeric xylose and mainly adopt polyacrylamide
The media such as amine gel, activated carbon separate eluting and enzymes biocatalysis and the technology preparation such as are combined with membrance separation：Patent CN
1847254A adopts polyacrylamide gel for chromatography media eluting, separation, prepares the oligomeric xylose of the various degree of polymerization；Patent
One kind is described in CN101632877A to be isolated and purified using each one-component of Xylo-oligosaccharide on Active Carbon, is prepared various
Degree of polymerization oligomeric xylose；The technology that patent 102796783A is combined using multi-functional carbohydrase living things catalysis with membrance separation, prepares poly-
The right function glucan product for 3-8.Separation differentiation step is comparatively laborious in these preparation methoies, be not also suitable for industrialization
Production application.Patent CN1364911A produces high vigor by control trichoderma reesei producing enzyme early stage and the reaction condition in later stage
Xylanase, using the xylanase enzymolysis xylan oligomeric xylose of the degree of polymerization for 2-5 is prepared.But, in the preparation method
Initial feed is xylan, is obtained through Methods For Purifications such as ethanol precipitation, ultrafiltration, nanofiltration column chromatographies, limits answering for the technology
Use scope.
The content of the invention
To make up the defect of prior art, the present invention provides a kind of preparation method of low polymerization degree oligomeric xylose.The present invention
Using pre-treating technology and zymolysis technique, with reference to electrodialysis methods to oligomeric xylose liquid purified treatment, can reduce resin processed
Soda acid loss in journey, while can recycle to the residual liquid after electrodialysis, prepares to the beneficial propagation of the functional bifidus factor, life
The low polymerization degree oligomeric xylose that production. art is simple, production efficiency is high, not only can improve oligosaccharide quality, and can improve life
Produce efficiency.
For achieving the above object, the present invention adopts following technical proposals：
A kind of a first aspect of the present invention, there is provided preparation method of low polymerization degree oligomeric xylose, comprises the steps：
(1) with wood fiber biomass as raw material, through diluted alkaline/hot water pre-treatment, filter liquid adopts high temperature to solid phase
Degraded or steam explosion pretreatment mode, by the long chain degradation of hemicellulose in wood fibre matter raw material so as in xylan
Dissolution, the Xylose Content after pretreatment in feed liquid is controlled in 5-20%；Cooling is stand-by；
(2) pretreatment fluid in step (1) adds the xylanase of wood fiber biomass dry weight 0.01-0.03% to carry out
Enzyme digestion reaction；
(3) enzymolysis solution Jing enzyme denaturing, purification, concentration, dried prepare low polymerization degree oligomeric xylose.
Preferably, wood fiber biomass includes in the step (1)：Cotton seed hullss, corn cob, corn straw, bagasse
Or one or more in wheat bran；Further preferably the wood fiber biomass is corn cob；
Preferably, wood fiber biomass raw material pre-treating method is specially in the step (1)：
Hot-water process：The wood fiber biomass raw material and fresh water (FW) are pressed into 1:(6-12) mass ratio mixing, 50-100
DEG C hot water treatment 30min-90min, filter liquid；
Diluted alkaline method：The wood fiber biomass raw material and fresh water (FW) are pressed into 1:(6-12) mass ratio mixing, adds institute
State the aqueous slkali of wood fiber biomass raw material dry weight percentage 0.01-0.5%, 50-100 DEG C of hot water treatment 30min-90min,
Filter liquid, and it is washed to solid phase close pH to 7；Wherein alkali for sodium hydroxide, potassium hydroxide, ammonia or Sodamide. one kind or
It is various；
The pretreatment mode also includes other with pre-treating technology of the present invention with effects equivalent；
Preferably, carry out high temperature degradation method to solid phase in the step (1) to be specially：Solid phase presses 1 with fresh water (FW):(6-
12) mass ratio mixing adds the weak acid catalyst that solid phase dry weight percentage is 0.3-1.5%, in 160-170 DEG C of steaming and decocting
30min-90min；
Preferably, carry out steam explosion method to solid phase in the step (1) to be specially：Solid phase presses 1 with fresh water (FW):(1-
5) mass ratio mixing, the weak acid catalyst of addition solid phase dry weight percentage 0.3-1.2%, processing pressure 1.5-2.5MPa, when
Between 30s-300s；
Weak acid catalyst is acetic acid, formic acid, citric acid, lactic acid, Asia in above-mentioned high temperature degradation method and steam blasting method
One or more in sulphuric acid or carbonic acid；
The pretreatment mode also includes other with pretreatment biodegrading process of the present invention with effects equivalent；
It is further preferred that Xylose Content computational methods are in the feed liquid：HPLC detects that the peak area of xylose component is accounted for
The percentage ratio of total sugar peak area；Testing conditions are：Chromatographic column is Shodex sugar KS-802, or other have equal analysis
The chromatographic column of effect；Mobile phase is ultra-pure water, 80 DEG C of column temperature, flow velocity 0.5-0.7ml/min；
Preferably, concretely comprising the following steps in the step (1)：
With cotton seed hullss as raw material, Jing diluted alkalines method carries out pre-treatment, and filter liquid adopts steam explosion pretreatment side to solid phase
Formula, by the long chain degradation of hemicellulose in wood fibre matter raw material so as in xylan dissolution, the wood after pretreatment in feed liquid
Sugared content control is lowered the temperature stand-by in 5-20%；
Wherein, the diluted alkaline method is by 1 by cotton seed hullss and fresh water (FW):(6-12) mass ratio mixing, adds the Semen Gossypii
The aqueous slkali of husk as raw material dry weight percentage 0.01-0.5%, 50-100 DEG C of hot water treatment 30min-90min, filter liquid, and water
It is washed till solid phase close pH to 7；Wherein described alkali is sodium hydroxide；
The steam explosion pretreatment concrete grammar is：Solid phase presses 1 with fresh water (FW):(1-5) mass ratio mixing, adds solid
The weak acid catalyst of phase dry weight percentage 0.3-1.2%, processing pressure 1.5-2.5MPa, treatment temperature is 150-190 DEG C, the time
30s-300s；Wherein described weak acid catalyst is acetic acid；
The present invention is adopted and carries out pre-treatment to oligomeric xylose with reference to steam explosion with diluted alkaline method, and inventor is in practical studies
Middle discovery, diluted alkaline method effectively can remove the acetyl group in the xylan backbone in raw material, glucosyl group, aralino,
So that product contents of monosaccharides is greatly lowered, be conducive to improving product low polymerization degree xylan proportion；Meanwhile, by above-mentioned
Adding proportion so that add the pH value of solution after alkali to maintain between 8-10, xylan is caused so as to be prevented effectively from because alkalescence is too high
Structural deterioration reduces oligomeric xylose yield；Also avoid leading to not because alkalescence is too low simultaneously playing removal miscellaneous sugar chain, acetyl group and
The action effect of a small amount of lignin and colloid；Then further machinery is carried out to plant cell wall by steam explosion broken
It is bad, so that xylan is further dissolved in extracting solution, simultaneously as early stage is through the process of diluted alkaline method and in steam explosion
Weak acid catalyst is added in method, so as to effectively reduce steam explosion reaction temperature and pressure, it is to avoid extracting solution color is deeper；
Meanwhile, inventor is had been surprisingly found that through this index of pretreated Xylose Content for control wood in practical studies
Polyose degradation degree and result of extraction are extremely important；When Xylose Content is too high (＞ 20%), although the degree of dissociation of xylan is carried
Height, but by-product showed increased, cause the oligomeric xylose content ratio that follow-up enzymolysis process is obtained to reduce, and pretreated
During Xylose Content relatively low (＜ 5%), xylan degree of dissociation is low, is unfavorable for the zymolysis of follow-up xylanase, again results in
Follow-up oligomeric xylose yield is reduced；And, after pre-treatment and pretreating process, follow-up enzymolysis time is reduced, and, wood is poly-
Carbohydrase usage amount is reduced, and is conducive to industrialized production.
Preferably, xylanase is that trichoderma reesei, green whip be mould or other bacterium with effects equivalent in the step (2)
Strain carries out fermentable and processes the β-Isosorbide-5-Nitrae-internal cutting type xylanase for preparing, and separately has xylobiase, α-L-arabinose glycosides
The enzyme system assosting effect with degradation of hemicellulose such as enzyme；
Preferably, purifying step includes activated carbon decolorizing and electrodialysis desalination in the step (3), wherein the activated carbon
For 50-90 DEG C, activated carbon addition is 0.1-2% to the bleaching temperature that decolouring is adopted, and bleaching time is 20-90min, decolourizes to complete
Plate-and-frame filtration afterwards；Feed liquid electrical conductivity≤800 μ s/cm up to standard after electrodialysis desalination, pH=4-8,15-42 DEG C of electric osmose eutectoid temperature, its
The mould difference of cationic exchange membrane and anion exchange membrane is 0.2-0.8MPa, and anode chamber and cathode chamber residual liquid carry out reclaiming profit
With.
The second aspect of the invention, discloses the low polymerization degree oligomeric xylose that above-mentioned preparation method is prepared.On Jing
State the oligomeric xylose that preparation method is obtained, the degree of polymerization (Degree of Polymerization, abbreviation DP) is between 2-6, low
Xylan purity >=75%, wherein degree of polymerization 2-4 oligomeric xylose purity >=70%, while study finding, the oligomeric xylose is not
Containing arabinose, acetyl base side chain, structural formula is as follows：Wherein n=0～4, A, B, C and D correspond respectively to two-dimentional nuclear-magnetism and are total to
4 end groups shaken in wave spectrum analysis；
The third aspect of the invention, discloses above-mentioned low polymerization degree oligomeric xylose in animal bifidobacteria and cheese breast bar
Application in bacterium propagation.Due to preparation method difference, the purity of oligomeric xylose, each degree of polymerization in resulting oligomeric xylose product
Oligomeric xylose ratio, the component ratio of impurity are simultaneously differed, prior art do not provide structure belonging to the present invention and/composition it is low
Xylan, and the oligomeric xylose product that the present invention is prepared, experiment proves that, animal bifidobacteria and lactobacillus casei are increased
Grow with good effect.
Beneficial effects of the present invention are：
The present invention can effectively improve xylose dissolution and degraded effect by pre-treatment and the synergism of pretreating process
Really, while effectively reducing extracting solution colourity and by-product generation, and xylanase addition can be significantly reduced, shortens enzyme digestion reaction
Time, improve product purity；
The present invention, using the application of electrodialysis methods substitutional ion exchanger resin, reduces soda acid consumption and resin in preparation
Regeneration, saves production cost；The preparation of one-step method low polymerization degree oligomeric xylose, reduces polyacrylamide in traditional preparation methods and coagulates
The eluting such as glue, activated carbon, membrance separation, chromatographic isolation, separating step, Simplified flowsheet.
In a word, the present invention is by each processing step of reasonably optimizing, and it is low to finally give the oligomeric xylose degree of polymerization, with 1,4-.beta.-Xylobiose,
Based on xylotriose and Xylotetrose., so as to strengthen it as the feature of bifidus factor, experiment proves that, it is preparation-obtained oligomeric
Xylose is good to animal bifidobacteria and lactobacillus casei cultivation effect.In a word, this invention simplifies technique, improving product quality,
Have a good application prospect.
Specific embodiment
Further detailed description, but embodiments of the present invention not limited to this are done to the present invention with reference to embodiment.
Embodiment 1
Cotton seed hullss 450g is taken, with the sodium hydroxide solution that concentration is 0.05% by 1:7 mass ratio mixing, is warming up to 60 and takes the photograph
Family name's degree is incubated 30min, filters liquid；One time is washed to pH value neutrality；1 is pressed with fresh water (FW):3 mass ratio mixing, adds Semen Gossypii
The weak acid catalyst (acetic acid) of shell dry weight percentage 0.7%, Steam explosion treatment, processing pressure 1.5-2.5MPa, time 120s；
Xylose Content in air blasting liquid is 5.85%, is lowered the temperature stand-by.0.8g xylanase is added to cooking liquor, enzyme digestion reaction temperature is 60
DEG C, enzymolysis time is 8h, and enzymolysis solution Jing enzyme denaturing, activated carbon decolorizing, electrodialysis desalination, concentration prepare low polymerization degree oligomeric xylose.
For 50-90 DEG C, activated carbon addition is 0.1-2% to the bleaching temperature that the activated carbon decolorizing is adopted, and bleaching time is 20-
90min, plate-and-frame filtration after the completion of decolouring；Feed liquid electrical conductivity≤800 μ s/cm up to standard, pH=4-8, electrodialysis after electrodialysis desalination
The mould difference of temperature 15-42 DEG C, wherein cation exchange membrane and anion exchange membrane is 0.2-0.8MPa, anode chamber and cathode chamber
Residual liquid is recycled.
Low polymerization degree oligomeric xylose constituent content table in the embodiment 1 of table 1
As shown in Table 1：In the product of embodiment 1, low polymerization degree 2-6 oligomeric xyloses purity is 87.21%, without wooden seven sugar etc.
High polymerization degree oligomeric xylose, and degree of polymerization 2-4 xylan purity is 83.04%.
Embodiment 2
Corn cob 450g is taken, with fresh water (FW) 1 is pressed:6 mass ratio mixing is warming up to 80 degrees Celsius of insulation 40min, filters liquid
Body；The acetic acid that corn cob dry weight percentage is 0.8%, high temperature steaming is added to process, treatment conditions are：165 DEG C of boiling temperature, steams
Time 50min is boiled, by the xylan dissolution in corn cob, the Xylose Content in cooking liquor is 15.75%, is lowered the temperature stand-by.Xiang Zheng
Boil liquid adds 0.1g xylanase, and enzyme digestion reaction temperature is 80 DEG C, and enzymolysis time is 8h, enzymolysis solution Jing enzyme denaturing, activated carbon decolorizing,
Electrodialysis desalination, concentration prepare low polymerization degree oligomeric xylose.The bleaching temperature that the activated carbon decolorizing is adopted is living for 50-90 DEG C
Property charcoal addition be 0.1-2%, bleaching time is 20-90min, plate-and-frame filtration after the completion of decolouring；Material up to standard after electrodialysis desalination
Liquid electrical conductivity≤800 μ s/cm, pH=4-8,15-42 DEG C of electric osmose eutectoid temperature, wherein cation exchange membrane and anion exchange membrane
Mould difference is 0.2-0.8MPa, and anode chamber and cathode chamber residual liquid are recycled.
Low polymerization degree oligomeric xylose constituent content table in the embodiment 2 of table 2
As shown in Table 2：In the product of embodiment 2, low polymerization degree oligomeric xylose purity is 79.16%, contour without the sugar of wood seven
Degree of polymerization oligomeric xylose, and degree of polymerization 2-4 xylan purity is 71.18%.
Embodiment 3
Wheat straw 450g is taken, with fresh water (FW) 1 is pressed:10 mass ratio mixing is warming up to 90 degrees Celsius of insulation 60min, filters liquid
Body；1 is pressed with fresh water (FW):8 mass ratio mixing, adds the acetic acid that wheat straw dry weight percentage is 1.2%, high temperature steaming process, place
Manage bar part is：165 DEG C of boiling temperature, digestion time 60min, by the xylan dissolution in wheat straw, the Xylose Content in cooking liquor
For 19.96%, lower the temperature stand-by.0.4g xylanase is added to cooking liquor, enzyme digestion reaction temperature is 80 DEG C, and enzymolysis time is 8h,
Enzymolysis solution Jing enzyme denaturing, activated carbon decolorizing, electrodialysis desalination, concentration prepare low polymerization degree oligomeric xylose.The activated carbon decolorizing is adopted
Bleaching temperature is 50-90 DEG C, and activated carbon addition is 0.1-2%, and bleaching time is 20-90min, sheet frame after the completion of decolouring
Filter；Feed liquid electrical conductivity≤800 μ s/cm up to standard after electrodialysis desalination, pH=4-8,15-42 DEG C of electric osmose eutectoid temperature, its middle-jiao yang, function of the spleen and stomach from
The mould difference of proton exchange and anion exchange membrane is 0.2-0.8MPa, and anode chamber and cathode chamber residual liquid are recycled.
Low polymerization degree oligomeric xylose constituent content table in the embodiment 3 of table 3
As shown in Table 3：In the product of embodiment 3, low polymerization degree oligomeric xylose purity is 75.09%, without the sugar of wood seven, wood six
The high polymerization degree oligomeric xylose such as sugar, and degree of polymerization 2-4 xylan purity is 72.11%.
Comparative example 1
Method only difference is that and save diluted alkaline method this process step with embodiment 1, that is, save and " be with concentration
0.05% sodium hydroxide solution presses 1:7 mass ratio mixing, is warming up to 60 degrees Celsius of insulation 30min, filters liquid；Washing one
All over neutral to pH value " step.
Low polymerization degree oligomeric xylose constituent content table in the comparative example 1 of table 4
As shown in Table 4：In the product of comparative example 1, low polymerization degree 2-6 oligomeric xyloses purity is 77.79%, sugared containing wood seven, is gathered
Right 2-4 xylan purity is 73.51%.
Embodiment 4
Low polymerization degree oligomeric xylose to obtaining in embodiment 1-3 and comparative example 1 carries out the external of lactobacillus casei propagation
Anaerobic culturel is tested：By culture medium based on the MRS culture medium for removing glucose, different oligomeric xyloses are added to replace in culture medium
For glucose as oligomeric xylose culture medium.With basal medium as control, using turbidimetry for Determination increment, in different cultures
Time surveys its OD600nm, draws growth curve.
Activated spawn：The Freezing Glycerine pipe lactobacillus strain of -85 DEG C of preservations is taken, in being inoculated into MRS fluid mediums, inoculation
Amount 1%, 36 DEG C of culture 36h.
Lactobacillus casei is bred：The syrup for having sterilized and glucose are added in corresponding fluid medium, Ran Houxiang
The lactobacillus casei that each inoculation of medium is activated, inoculum concentration 1%, 36 DEG C of culture 36h.
Can be seen that by in-vitro multiplication experiment, low polymerization degree oligomeric xylose is more oligomeric than common to the cultivation effect of lactobacillus casei
Xylose effect is good, and embodiment 1-3 cultivation effect is better than comparative example 1.
Embodiment 5
Low polymerization degree oligomeric xylose to obtaining in embodiment 1-3 and comparative example 1 carries out the body of animal bifidobacteria propagation
Outer Anaerobic culturel experiment：By culture medium based on the MRS culture medium for removing glucose, different oligomeric xyloses are added in culture medium
Glucose is substituted as oligomeric xylose culture medium.With basal medium as control, using turbidimetry for Determination increment, in different trainings
The foster time surveys its OD600nm, draws growth curve.
Activated spawn：The Freezing Glycerine pipe strain of -85 DEG C of preservations is taken, in being inoculated into basal medium, inoculum concentration 1%, 36
DEG C culture 36h.
Animal bifidobacteria is bred：The oligosaccharide for having sterilized and glucose are added in corresponding fluid medium, so
The animal bifidobacteria that backward each inoculation of medium is activated, inoculum concentration 1%, 36 DEG C of culture 48h.
Can be seen that by in-vitro multiplication experiment, low polymerization degree oligomeric xylose is lower than common to the cultivation effect of animal bifidobacteria
Xylan effect is good, and embodiment 1-3 cultivation effect is better than comparative example 1.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention not by above-described embodiment
Limit, one of ordinary skill in the art should be understood that on the basis of technical scheme, those skilled in the art need not
The various modifications made by paying creative work or deformation are still in protection scope of the present invention.
Claims (10)
1. a kind of preparation method of low polymerization degree oligomeric xylose, it is characterised in that comprise the steps：
(1) with wood fiber biomass as raw material, through diluted acid/diluted alkaline/hot water pre-treatment, filter liquid, to solid phase using high
Temperature drop solution or steam explosion pretreatment mode, lower the temperature stand-by；
(2) pretreatment fluid in step (1) adds xylanase to carry out enzyme digestion reaction；
(3) enzymolysis solution Jing enzyme denaturing, purification, concentration, dried prepare low polymerization degree oligomeric xylose.
2. a kind of preparation method of low polymerization degree oligomeric xylose as claimed in claim 1, it is characterised in that the step (1)
Middle wood fiber biomass is one or more in cotton seed hullss, corn cob, corn straw, bagasse or wheat bran.
3. a kind of preparation method of low polymerization degree oligomeric xylose as claimed in claim 1, it is characterised in that the step (1)
Middle wood fiber biomass raw material pre-treating method is specially：
Hot-water process：The wood fiber biomass raw material and fresh water (FW) are pressed into 1:(6-12) mass ratio mixing, 50-100 DEG C of heat
Water process 30min-90min, filter liquid；
Diluted alkaline method：The wood fiber biomass raw material and fresh water (FW) are pressed into 1:(6-12) mass ratio mixing, adds the wood
The aqueous slkali of matter fibrous biomass raw material dry weight percentage 0.01-0.5%, 50-100 DEG C of hot water treatment 30min-90min is filtered
Liquid, and it is washed to solid phase close pH to 7；Wherein alkali is one kind or many of sodium hydroxide, potassium hydroxide, ammonia or Sodamide.
Kind.
4. a kind of preparation method of low polymerization degree oligomeric xylose as claimed in claim 1, it is characterised in that the step (1)
In high temperature degradation method carried out to solid phase be specially：Solid phase presses 1 with fresh water (FW):(6-12) mass ratio mixing adds solid phase dry weight
Percentage ratio is the weak acid catalyst of 0.3-1.5%, in 160-170 DEG C of steaming and decocting 30min-90min；
Steam explosion method is carried out in the step (1) to solid phase to be specially：Solid phase presses 1 with fresh water (FW):(1-5) mass ratio is mixed
Close, add the weak acid catalyst of solid phase dry weight percentage 0.3-1.2%, processing pressure 1.5-2.5MPa, time 30s-300s；
Weak acid catalyst in the high temperature degradation method is in acetic acid, formic acid, citric acid, lactic acid, sulfurous acid or carbonic acid
Plant or various；
Weak acid catalyst in the steam explosion method is in acetic acid, formic acid, citric acid, lactic acid, sulfurous acid or carbonic acid
Plant or various.
5. a kind of preparation method of low polymerization degree oligomeric xylose as claimed in claim 1, it is characterised in that the step (1)
Xylose Content after middle pretreatment in feed liquid is 5-20%；
Xylose Content computational methods are in the feed liquid：HPLC detects that the peak area of xylose component accounts for the percentage of total sugar peak area
Than.Testing conditions are：Chromatographic column is Shodex sugar KS-802, or other have the chromatographic column of equal analytical effect；Flowing
It is mutually ultra-pure water, 80 DEG C of column temperature, flow velocity 0.5-0.7ml/min.
6. a kind of preparation method as claimed in claim 1, it is characterised in that the step (1) concretely comprises the following steps：With cotton seed hullss
For raw material, Jing diluted alkalines method carries out pre-treatment, and filter liquid adopts steam explosion pretreatment mode, by wood fibre matter to solid phase
The long chain degradation of hemicellulose in raw material so as in xylan dissolution, Xylose Content in feed liquid is controlled in 5- after pretreatment
20%, lower the temperature stand-by；
Wherein, the diluted alkaline method is by 1 by cotton seed hullss and fresh water (FW):(6-12) mass ratio mixing, adds the cotton seed hullss former
The aqueous slkali of material dry weight percentage 0.01-0.5%, 50-100 DEG C of hot water treatment 30min-90min, filter liquid, and be washed to
Solid phase close pH to 7；Wherein described alkali is sodium hydroxide；
The steam explosion pretreatment concrete grammar is：Solid phase presses 1 with fresh water (FW):(1-5) mass ratio mixing, adds solid phase to do
The weak acid catalyst of weight percentage ratio 0.3-1.2%, processing pressure 1.5-2.5MPa, treatment temperature is 150-190 DEG C, time 30s-
300s；Wherein described weak acid catalyst is acetic acid.
7. a kind of preparation method as claimed in claim 1, it is characterised in that xylanase addition is in the step (2)
Wood fiber biomass dry weight 0.01-0.03%.
8. a kind of preparation method as claimed in claim 1, it is characterised in that purifying step includes activity in the step (3)
Carbon decoloring and electrodialysis desalination, wherein the bleaching temperature that the activated carbon decolorizing is adopted is for 50-90 DEG C, activated carbon addition is
0.1-2%, bleaching time is 20-90min, plate-and-frame filtration after the completion of decolouring；Feed liquid electrical conductivity≤800 up to standard after electrodialysis desalination
μ s/cm, pH=4-8, the mould difference of 15-42 DEG C of electric osmose eutectoid temperature, wherein cation exchange membrane and anion exchange membrane is 0.2-
0.8MPa, anode chamber and cathode chamber residual liquid are recycled.
9. the low polymerization degree oligomeric xylose that the preparation method described in any one of claim 1-8 is prepared, the low polymerization degree
The degree of polymerization of oligomeric xylose be 2-6 between, oligomeric xylose purity >=75%, the wherein degree of polymerization for 2-4 oligomeric xylose purity >=
70%.
10. application of the low polymerization degree oligomeric xylose described in claim 9 in animal bifidobacteria and lactobacillus casei propagation.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000236899A (en) * | 1998-12-24 | 2000-09-05 | Asahi Chem Ind Co Ltd | Production of xylose and xylooligosaccharide |
| CN101434977A (en) * | 2008-12-23 | 2009-05-20 | 中国石油化工股份有限公司 | Novel method for saccharification of ligno-cellulose |
| CN103333932A (en) * | 2012-12-29 | 2013-10-02 | 青岛蔚蓝生物集团有限公司 | A method for preparing xylooligosaccharide |
| CN104593446A (en) * | 2014-12-25 | 2015-05-06 | 山东龙力生物科技股份有限公司 | Preparation method of pure xylooligosaccharide powder |
-
2016
- 2016-12-28 CN CN201611237155.2A patent/CN106636256B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000236899A (en) * | 1998-12-24 | 2000-09-05 | Asahi Chem Ind Co Ltd | Production of xylose and xylooligosaccharide |
| CN101434977A (en) * | 2008-12-23 | 2009-05-20 | 中国石油化工股份有限公司 | Novel method for saccharification of ligno-cellulose |
| CN103333932A (en) * | 2012-12-29 | 2013-10-02 | 青岛蔚蓝生物集团有限公司 | A method for preparing xylooligosaccharide |
| CN104593446A (en) * | 2014-12-25 | 2015-05-06 | 山东龙力生物科技股份有限公司 | Preparation method of pure xylooligosaccharide powder |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439695A (en) * | 2018-08-31 | 2019-03-08 | 山东龙力生物科技股份有限公司 | A kind of method of industrial waste coproduction xylo-oligosaccharide and xylose, xylitol |
| CN109439695B (en) * | 2018-08-31 | 2021-08-17 | 山东龙力生物科技股份有限公司 | Method for co-producing xylooligosaccharide, xylose and xylitol by industrial waste |
| CN109055227A (en) * | 2018-09-27 | 2018-12-21 | 广州市金因源生物技术有限公司 | The protective agent and its method for preserving of genetic engineering bacterium strain freeze-drying preservation |
| CN109055227B (en) * | 2018-09-27 | 2022-06-10 | 广州市金因源生物技术有限公司 | Protective agent for freeze-drying preservation of genetically engineered strain and preservation method thereof |
| CN109845958A (en) * | 2019-04-03 | 2019-06-07 | 沈阳师范大学 | A kind of wheat bran stabilizes and the method for quality improving |
| CN110004194A (en) * | 2019-04-18 | 2019-07-12 | 四川金象赛瑞化工股份有限公司 | A method of production xylose and furfural are digested using bagasse |
| CN109957982B (en) * | 2019-04-24 | 2020-11-17 | 吴学军 | Programmed explosion pulping method for crop straws |
| CN109957982A (en) * | 2019-04-24 | 2019-07-02 | 吴学军 | A kind of procedural blast pulping method of agricultural crop straw |
| CN110256510A (en) * | 2019-06-06 | 2019-09-20 | 南京林业大学 | A method of producing multicomponent xylo-oligosaccharide |
| CN110973421A (en) * | 2019-11-29 | 2020-04-10 | 中国农业科学院农产品加工研究所 | High-content xylo-oligosaccharide corn bamboo shoot beverage and preparation method thereof |
| CN111004827A (en) * | 2019-12-27 | 2020-04-14 | 山东百龙创园生物科技股份有限公司 | Preparation method of xylo-oligosaccharide |
| CN111575328A (en) * | 2020-05-22 | 2020-08-25 | 南京林业大学 | Method for preparing xylo-oligosaccharide by coupling acid hydrolysis and enzyme hydrolysis |
| CN113151614A (en) * | 2021-03-17 | 2021-07-23 | 南京林业大学 | Method for preparing xylooligosaccharide from agricultural and forestry waste through two-step acetic acid hydrolysis |
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