CN104292356A - Method for extracting peony polysaccharide from peony cakes with biological enzyme method - Google Patents
Method for extracting peony polysaccharide from peony cakes with biological enzyme method Download PDFInfo
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Abstract
The invention belongs to the technical field of extraction of polysaccharides and particularly relates to a method for extracting a peony polysaccharide from peony cakes with a biological enzyme method. The method for extracting the peony polysaccharide from the peony cakes with the biological enzyme method comprises the steps as follows: (1) pretreatment of raw materials; (2) enzymolysis; (3) water-insoluble polysaccharide conversion; (4) centrifugal separation; (5) soluble polysaccharide extraction. Adopted enzymes are mild in action condition, various enzymes are adopted for performing enzymolysis on fat and cellulose in the peony cakes to extract the polysaccharide in the fat and the cellulose, the utilization rate of the peony cakes is increased, and the peony polysaccharide obtained with the method is high in yield and purity.
Description
Technical field
The invention belongs to technical field of polysaccharide extraction, be specifically related to a kind of method that biological enzyme extracts tree peony polysaccharide from peony meal.
background technology
Tree peony belongs to Dicotyledoneae, and Saxifragales Paeoniaceae Paeonia, has the laudatory title of the king in spending, and can set up Botanical Garden in park and landscaped area.
SARKER SDW etc. are studied the chemical composition of tree peony and Paeonia lactiflora seed and pharmacologically active, therefrom be separated and obtain 9 kinds of stilbene constituents, wherein suffruticosolA, B and C be composition specific to Paeoniaceae, pharmacologically active shows, these 9 kinds of stilbene constituents have the effects such as anticancer, anti-oxidant, anti-inflammatory.KLM HJH etc. passes through chemical constitution study, find that Paeonia suffruticosa seed water-soluble portion contains higher monoterpene glycosides, particularly paeoniflorin content is higher, and a large amount of carbohydrates, higher at middle polarity part stilbene compound content, plum fine jades etc. find tree peony kind Renhe seed coat site analysis, and it contains a large amount of unsaturated fatty acidss, and particularly linoleic acid content is higher.Polysaccharide is at Organism immunoregulation, and there is stronger activity hypoglycemic, anti-ageing aspect, and Zhang Ming thinks polysaccharide as activeconstituents because its content is less, impurity is many, and therefore in leaching process, influence factor is complicated, as extraction time, solid-liquid ratio, extraction time, alcohol precipitation ratio etc.
CN103045372B discloses the method for comprehensive utilization of peony meal, and the method comprises following step a. peony meal pulverizer and is crushed to 40 orders, dress extraction kettle, and described peony meal is that peony seeds benevolence just squeezes rear remaining residual dregs of rice slag;
B. use food-class CO 2, its purity is 99.99%, and flow is 12L/ hour, and setting extracting pressure is 20MPa, temperature
Degree is 25 DEG C, and separation condition is: separator I pressure 10KPa, temperature 50 C, separator II pressure 4KPa, temperature 40 DEG C, extraction time 0.5 hour;
C. extract complete, collect extract, obtain peony seed oil;
D. getting residue processed remaining in step c is 20% to its moisture content, obtains peony meal;
The tree peony dregs of rice obtained in steps d extract polysaccharide, the peony meal of pulverizing is inserted in multi-function extractor, add the water of raw material 10 times amount, in extractor chuck, pass into saturated vapor, vapor pressure is 2-2.5kg, unlatching motor stirs, after seething with excitement, vapor pressure is dropped to 1.5-2kg, keep boiling state, and close agitator motor, carry out air distillation;
Collect distillate, will slip out liquid and flow in coarse crystallization tank by pipeline, open motor and stir cooling, coarse crystallization tank presss from both sides
Circulating brine temperature in cover is-2--5 DEG C, and till occurring without white opacity liquid in distillate, that now collects distillates
Liquid weight is about the 5-10 of grouts amount doubly; Can obtain Crude polysaccharides, crude product, after sugared content, determining the protein quantity, adds water again
Dissolve, the alcohol adding 0.5 times of volume shakes up, and leaves standstill in a moment, the centrifugal 15min of 6000r/min, removing protein impurities;
Add alcohol again in supernatant liquor, to volume fraction of ethanol about 60%, shake up rear leaving standstill, after to be precipitated, carefully by supernatant
Liquid is poured out, and adds appropriate 90% alcohol and dehydrated alcohol 2 dehydrations, cross filter cake and wash 2 times with dehydrated alcohol again in precipitation,
Spread out on plate, 50 DEG C of oven dry, obtain polysaccharide products.
Above-mentioned method is mainly raw material with peony meal, further supercritical carbon dioxide extraction, extract peony seed oil, and then to residue processed, with the peony meal obtained for raw material extracts polysaccharide, its extracting method is hot water extraction.
Need to improve for above-mentioned method, design a kind of method that extraction yield is high.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of method that yield and the higher biological enzyme of purity extract tree peony polysaccharide from peony meal.
The method that biological enzyme of the present invention extracts tree peony polysaccharide from peony meal is realized by following technical scheme:
Biological enzyme extracts a method for tree peony polysaccharide from peony meal, comprises following step:
(1) raw materials pretreatment
Peony meal drying is 2-4% to its moisture content, then is crushed to 80-200 order;
(2) enzymolysis
By the peony meal after pulverizing, add water mixing, the part by weight of peony meal and water is 1:6-12, and adds lipase, the addition 0.3-0.5% of lipase, hydrolysis temperature 45 DEG C, adjust pH 5.5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, the power of microwave is 400-600W, frequency is 800MHZ, enzymolysis 0.1-0.5h;
Add cellulase again, the addition of cellulase is 0.4-0.6%, hydrolysis temperature 40 DEG C, pH value 5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, and the power of microwave is 400-600W, and frequency is 800MHZ, enzymolysis 0.1-0.5h;
Add Sumizyme MP again, the addition of Sumizyme MP is 0.4-1.0%, hydrolysis temperature is 45 DEG C, pH value 8.2, above-mentioned raw material is placed in Microwave Extraction Equipment extract, the power of microwave is 400-600W, and frequency is 800MHZ, enzymolysis 0.1-0.5h, go out after enzymolysis enzyme 5-10min at 100-105 DEG C;
(3) water-insoluble polysaccharide conversion
Diastatic addition is 1.0-1.2%, hydrolysis temperature 40 DEG C, pH value 5, after enzymolysis 1.5-2.0h, and the 100-105 DEG C of enzyme 5-10min that goes out;
(4) centrifugation
By the peony meal after enzymolysis, centrifugal 10-20 min, centrifugal rotational speed is 4500 rpm, supernatant liquor and precipitation is collected respectively;
(5) extraction of soluble polysaccharide
The supernatant liquid filtering got in step (4) removes slag, the filtrate obtained after filtration is concentrated at 60-70 DEG C, being concentrated into its concentration is 60-70%, concentrated solution adds in 1:4 ratio that 95% alcohol settling leaves standstill 10-20 hour, centrifugal 10-20 min, centrifugal rotational speed is 4500 rpm, and collecting precipitation also cleans 2-3 time repeatedly with ethanol, and lyophilize to moisture content is after 4-6%, pulverize at-1-4 DEG C again, obtain soluble polysaccharide;
Above-mentioned enzyme concentration is the weight percent that enzyme accounts for the peony meal after micronizing.
Preferably, the consumption of lipase is 0.4%.
Preferably, the consumption of cellulase is 0.5%.
Preferably, diastatic addition is 1.0%.
Preferably, a kind of biological enzyme extracts the method for tree peony polysaccharide from peony meal, comprises following step:
(1) raw materials pretreatment
Peony meal drying to its moisture content is 3%, then is crushed to 120 orders;
(2) enzymolysis
By the peony meal after pulverizing, add water mixing, the part by weight of peony meal and water is 1:8, and adds lipase, the addition 0.4% of lipase, hydrolysis temperature 45 DEG C, adjust pH 5.5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, the power of microwave is 500W, frequency is 800MHZ, enzymolysis 0.2h;
Add cellulase again, the addition of cellulase be 0.5%, hydrolysis temperature 40 DEG C, pH value 5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, the power of microwave is 500W, and frequency is 800MHZ, enzymolysis 0.2h;
Add aspartic protease and papoid again, the addition of aspartic protease is 0.8%, the addition of papoid is 0.5%, hydrolysis temperature is 45 DEG C, and above-mentioned raw material is placed in Microwave Extraction Equipment and extracts by pH value 5.0, the power of microwave is 500W, frequency is 800MHZ, enzymolysis 0.2h, and go out after enzymolysis enzyme 8min at 100 DEG C;
(3) water-insoluble polysaccharide conversion
Diastatic addition is 1%, hydrolysis temperature 40 DEG C, pH value 5, after enzymolysis 1.8h, and 100 DEG C of enzyme 6min that go out;
(4) centrifugation
By the peony meal after enzymolysis, centrifugal 15 min, centrifugal rotational speed is 4500 rpm, supernatant liquor and precipitation is collected respectively;
(5) extraction of soluble polysaccharide
The supernatant liquid filtering got in step (4) removes slag, the filtrate obtained after filtration is concentrated at 65 DEG C, being concentrated into its concentration is 65%, concentrated solution adds 95% alcohol settling in 1:4 ratio and leaves standstill 15 hours, centrifugal 15 min, centrifugal rotational speed is 4500 rpm, and collecting precipitation also cleans 2-3 time repeatedly with ethanol, and lyophilize to moisture content is after 5%, pulverize at-1-4 DEG C again, obtain soluble polysaccharide;
Above-mentioned enzyme concentration is the weight percent that enzyme accounts for the peony meal after micronizing.
Enzymolysis fat, fiber order are also innovations of the present invention, and due to by after fatty enzymolysis, grease can not be wrapped in outside peony meal, thus are conducive to various composition and contact with enzyme, thus the efficiency of raising enzymolysis; Therefore, the fatty enzymolysis Mierocrystalline cellulose again of first enzymolysis is conducive to the removal of fat and fiber;
Generally adopt the mode of organic solvent of adding to the treatment process of fat in usual way, although organic solvent is also comparatively thorough to the removal of grease, but the condition of its effect is stronger, and follow-up removal process is also more complicated, also easily causes the environmentally hazardous problem of organic solvent in addition; Organic solvent is adopted to remove its cost of grease in addition also higher;
The present invention adopts enzyme to act on to remain on the grease in peony meal, and action condition is gentle, and also comparatively thorough to the removal of grease, and there is not the problem that follow-up organic solvent reclaims and bring environmental pollution;
Add cellulase again and the cellulose degradation in peony meal is generated water-soluble glucose;
After the polysaccharide enzymolysis of non-solubility is soluble polysaccharide, carbohydrate is soluble in water, then gets supernatant liquor, filters, concentrated, alcohol precipitation, by Polyose extraction wherein out.
The selection of the kind of enzyme of the present invention and the selection of consumption and enzymolysis order is not accidental, but contriver has paid performing creative labour obtains, the adjustment of enzyme and various ratio all can affect the extraction effect of final polysaccharide, only have adopt the present invention to enzyme and the corresponding proportion of enzyme peony meal is processed, and carry out according to order of the present invention, just can obtain result of the present invention, enzyme be carried out replace or the replacement of enzyme-added order, all can not get maximum polysaccharide extract rate.
Therefore, adopt the method for freezing and pulverizing to extracting the polysaccharide obtained in the present invention, the quality maintaining polysaccharide is unaffected.
Beneficial effect of the present invention is, adopt enzyme action condition gentle, adopt various different enzyme by the fat in peony meal, cellulase hydrolysis, from peony meal, extract polysaccharide simultaneously, utilize peony meal to greatest extent, and the polysaccharide adopting method of the present invention to obtain, its yield and purity high.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described, so that those skilled in the art more understands the present invention, but does not therefore limit the present invention.
Following detection method, if no special instructions, adopts phend-sulphuric acid to survey the content of polysaccharide.
Embodiment 1
Biological enzyme extracts a method for tree peony polysaccharide from peony meal, comprises following step:
(1) raw materials pretreatment
Peony meal drying to its moisture content is 3%, then is crushed to 120 orders;
(2) enzymolysis
By the peony meal after pulverizing, add water mixing, the part by weight of peony meal and water is 1:8, and adds lipase, the addition 0.4% of lipase, hydrolysis temperature 45 DEG C, adjust pH 5.5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, the power of microwave is 500W, frequency is 800MHZ, enzymolysis 0.2h;
Add cellulase again, the addition of cellulase be 0.5%, hydrolysis temperature 40 DEG C, pH value 5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, the power of microwave is 500W, and frequency is 800MHZ, enzymolysis 0.2h;
Add Sumizyme MP again, the addition of Sumizyme MP is 0.8%, and hydrolysis temperature is 45 DEG C, pH value 8.2, is placed in Microwave Extraction Equipment by above-mentioned raw material and extracts, and the power of microwave is 500W, frequency is 800MHZ, enzymolysis 0.3h, and go out after enzymolysis enzyme 8min at 100 DEG C;
(3) water-insoluble polysaccharide conversion
Diastatic addition is 1%, hydrolysis temperature 40 DEG C, pH value 5, after enzymolysis 1.8h, and 100 DEG C of enzyme 6min that go out;
(4) centrifugation
By the peony meal after enzymolysis, centrifugal 15 min, centrifugal rotational speed is 4500 rpm, supernatant liquor and precipitation is collected respectively;
(5) extraction of soluble polysaccharide
The supernatant liquid filtering got in step (5) removes slag, the filtrate obtained after filtration is concentrated at 65 DEG C, being concentrated into its concentration is 65%, concentrated solution adds 95% alcohol settling in 1:4 ratio and leaves standstill 15 hours, centrifugal 15 min, centrifugal rotational speed is 4500 rpm, and collecting precipitation also cleans 2-3 time repeatedly with ethanol, and lyophilize to moisture content is after 5%, pulverize at-1-4 DEG C again, obtain soluble polysaccharide;
Above-mentioned enzyme concentration is the weight percent that enzyme accounts for the peony meal after micronizing.
The content adopting phend-sulphuric acid to record sugar in the polysaccharide of gained is 94.5%;
The extraction yield of polysaccharide is: 4.67%.
Embodiment 2
Biological enzyme extracts a method for tree peony polysaccharide from peony meal, comprises following step:
(1) raw materials pretreatment
Peony meal drying to its moisture content is 2%, then is crushed to 80 orders;
(2) enzymolysis
By the peony meal after pulverizing, add water mixing, the part by weight of peony meal and water is 1:6, and adds lipase, the addition 0.3% of lipase, hydrolysis temperature 45 DEG C, adjust pH 5.5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, the power of microwave is 400W, frequency is 800MHZ, enzymolysis 0.1h;
Add cellulase again, the addition of cellulase be 0.4%, hydrolysis temperature 40 DEG C, pH value 5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, the power of microwave is 400W, and frequency is 800MHZ, enzymolysis 0.1-0.5h;
Add Sumizyme MP again, the addition of Sumizyme MP is 0.4%, and hydrolysis temperature is 45 DEG C, pH value 8.2, is placed in Microwave Extraction Equipment by above-mentioned raw material and extracts, and the power of microwave is 400W, frequency is 800MHZ, enzymolysis 0.1h, and go out after enzymolysis enzyme 5min at 100 DEG C;
(3) water-insoluble polysaccharide conversion
Diastatic addition is 0.8%, hydrolysis temperature 40 DEG C, pH value 5, after enzymolysis 1.8h, and 100 DEG C of enzyme 6min that go out;
(4) centrifugation
By the peony meal after enzymolysis, centrifugal 15 min, centrifugal rotational speed is 4500 rpm, supernatant liquor and precipitation is collected respectively;
(5) extraction of soluble polysaccharide
The supernatant liquid filtering got in step (5) removes slag, the filtrate obtained after filtration is concentrated at 65 DEG C, being concentrated into its concentration is 65%, concentrated solution adds 95% alcohol settling in 1:4 ratio and leaves standstill 15 hours, centrifugal 15 min, centrifugal rotational speed is 4500 rpm, and collecting precipitation also cleans 2-3 time repeatedly with ethanol, and lyophilize to moisture content is after 5%, pulverize at-1-4 DEG C again, obtain soluble polysaccharide;
Above-mentioned enzyme concentration is the weight percent that enzyme accounts for the peony meal after micronizing.
The content adopting phend-sulphuric acid to record sugar in the polysaccharide of gained is 94.5%;
The extraction yield of polysaccharide is: 4.09%.
Embodiment 3
Biological enzyme extracts a method for tree peony polysaccharide from peony meal, comprises following step:
(1) raw materials pretreatment
Peony meal drying to its moisture content is 4%, then micronizing to 200 order;
(2) enzymolysis
By the peony meal after pulverizing, add water mixing, the part by weight of peony meal and water is 1:12, and adds lipase, the addition 0.5% of lipase, hydrolysis temperature 45 DEG C, adjust pH 5.5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, the power of microwave is 600W, frequency is 800MHZ, enzymolysis 0.5h;
Add cellulase again, the addition of cellulase be 0.6%, hydrolysis temperature 40 DEG C, pH value 5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, the power of microwave is 600W, and frequency is 800MHZ, enzymolysis 0.5h;
Add Sumizyme MP again, the addition of Sumizyme MP is 1.0%, and hydrolysis temperature is 45 DEG C, pH value 8.2, is placed in Microwave Extraction Equipment by above-mentioned raw material and extracts, and the power of microwave is 600W, frequency is 800MHZ, enzymolysis 0.5h, and go out after enzymolysis enzyme 10min at 100 DEG C;
(3) water-insoluble polysaccharide conversion
Diastatic addition is 1.2%, hydrolysis temperature 40 DEG C, pH value 5, after enzymolysis 1.8h, and 100 DEG C of enzyme 6min that go out;
(4) centrifugation
By the peony meal after enzymolysis, centrifugal 15 min, centrifugal rotational speed is 4500 rpm, supernatant liquor and precipitation is collected respectively;
(5) extraction of soluble polysaccharide
The supernatant liquid filtering got in step (5) removes slag, the filtrate obtained after filtration is concentrated at 65 DEG C, being concentrated into its concentration is 65%, concentrated solution adds 95% alcohol settling in 1:4 ratio and leaves standstill 15 hours, centrifugal 15 min, centrifugal rotational speed is 4500 rpm, and collecting precipitation also cleans 2-3 time repeatedly with ethanol, and lyophilize to moisture content is after 5%, pulverize at-1-4 DEG C again, obtain soluble polysaccharide;
Above-mentioned enzyme concentration is the weight percent that enzyme accounts for the peony meal after micronizing.
The content adopting phend-sulphuric acid to record sugar in the polysaccharide of gained is 94.5%;
The extraction yield of polysaccharide is: 4.54%.
Claims (5)
1. biological enzyme extracts a method for tree peony polysaccharide from peony meal, comprises following step:
(1) raw materials pretreatment
Peony meal drying is 2-4% to its moisture content, then is crushed to 80-200 order;
(2) enzymolysis
By the peony meal after pulverizing, add water mixing, the part by weight of peony meal and water is 1:6-12, and adds lipase, the addition 0.3-0.5% of lipase, hydrolysis temperature 45 DEG C, adjust pH 5.5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, the power of microwave is 400-600W, frequency is 800MHZ, enzymolysis 0.1-0.5h;
Add cellulase again, the addition of cellulase is 0.4-0.6%, hydrolysis temperature 40 DEG C, pH value 5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, and the power of microwave is 400-600W, and frequency is 800MHZ, enzymolysis 0.1-0.5h;
Add Sumizyme MP again, the addition of Sumizyme MP is 0.4-1.0%, hydrolysis temperature is 45 DEG C, pH value 8.2, above-mentioned raw material is placed in Microwave Extraction Equipment extract, the power of microwave is 400-600W, and frequency is 800MHZ, enzymolysis 0.1-0.5h, go out after enzymolysis enzyme 5-10min at 100-105 DEG C;
(3) water-insoluble polysaccharide conversion
Diastatic addition is 0.8-1.2%, hydrolysis temperature 40 DEG C, pH value 5, after enzymolysis 1.5-2.0h, and the 100-105 DEG C of enzyme 5-10min that goes out; Above-mentioned raw material is placed in Microwave Extraction Equipment extract, the power of microwave is 400-600W, and frequency is 800MHZ, enzymolysis 0.1-0.5h, and go out after enzymolysis enzyme 5-10min at 100-105 DEG C;
(4) centrifugation
By the peony meal after enzymolysis, centrifugal 10-20 min, centrifugal rotational speed is 4500 rpm, supernatant liquor and precipitation is collected respectively;
(5) extraction of soluble polysaccharide
The supernatant liquid filtering got in step (4) removes slag, the filtrate obtained after filtration is concentrated at 60-70 DEG C, being concentrated into its concentration is 60-70%, concentrated solution adds in 1:4 ratio that 95% alcohol settling leaves standstill 10-20 hour, centrifugal 10-20 min, centrifugal rotational speed is 4500 rpm, and collecting precipitation also cleans 2-3 time repeatedly with ethanol, and lyophilize to moisture content is after 4-6%, pulverize at-1-4 DEG C again, obtain soluble polysaccharide;
Above-mentioned enzyme concentration is the weight percent that enzyme accounts for the peony meal after micronizing.
2. a kind of biological enzyme extracts the method for tree peony polysaccharide from peony meal as claimed in claim 1, it is characterized in that, the consumption of described lipase is 0.4%.
3. a kind of biological enzyme as claimed in claim 1 extracts the method for tree peony polysaccharide from peony meal, it is characterized in that, the consumption of described cellulase is 0.5%.
4. a kind of biological enzyme as claimed in claim 1 extracts the method for tree peony polysaccharide from peony meal, it is characterized in that, described diastatic addition is 1.0%.
5. a kind of biological enzyme according to any one of claim 1-4 extracts the method for tree peony polysaccharide from peony meal, it is characterized in that, described method comprises following step:
(1) raw materials pretreatment
Peony meal drying to its moisture content is 3%, then is crushed to 120 orders;
(2) enzymolysis
By the peony meal after pulverizing, add water mixing, the part by weight of peony meal and water is 1:8, and adds lipase, the addition 0.4% of lipase, hydrolysis temperature 45 DEG C, adjust pH 5.5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, the power of microwave is 500W, frequency is 800MHZ, enzymolysis 0.2h;
Add cellulase again, the addition of cellulase be 0.5%, hydrolysis temperature 40 DEG C, pH value 5, above-mentioned raw material is placed in Microwave Extraction Equipment and extracts, the power of microwave is 500W, and frequency is 800MHZ, enzymolysis 0.2h;
Add Sumizyme MP again, the addition of Sumizyme MP is 0.8%, and hydrolysis temperature is 45 DEG C, pH value 8.2, is placed in Microwave Extraction Equipment by above-mentioned raw material and extracts, and the power of microwave is 500W, frequency is 800MHZ, enzymolysis 0.3h, and go out after enzymolysis enzyme 8min at 100 DEG C;
(3) water-insoluble polysaccharide conversion
Diastatic addition is 1%, hydrolysis temperature 40 DEG C, pH value 5, after enzymolysis 1.8h, and 100 DEG C of enzyme 6min that go out;
(4) centrifugation
By the peony meal after enzymolysis, centrifugal 15 min, centrifugal rotational speed is 4500 rpm, supernatant liquor and precipitation is collected respectively;
(5) extraction of soluble polysaccharide
The supernatant liquid filtering got in step (4) removes slag, the filtrate obtained after filtration is concentrated at 65 DEG C, being concentrated into its concentration is 65%, concentrated solution adds 95% alcohol settling in 1:4 ratio and leaves standstill 15 hours, centrifugal 15 min, centrifugal rotational speed is 4500 rpm, and collecting precipitation also cleans 2-3 time repeatedly with ethanol, and lyophilize to moisture content is after 5%, pulverize at-1-4 DEG C again, obtain soluble polysaccharide;
Above-mentioned enzyme concentration is the weight percent that enzyme accounts for the peony meal after micronizing.
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| CN105294881A (en) * | 2015-12-11 | 2016-02-03 | 苏州泽达兴邦医药科技有限公司 | Method for extracting crude peony pod polysaccharide of from peony pods |
| CN106749740A (en) * | 2016-12-31 | 2017-05-31 | 新昌县派特普科技有限公司 | A kind of method that polysaccharide is extracted in the dregs of rice from tree peony |
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