CN101595991A - The preparation method of canna edulis ker polysaccharide polyphenol complex - Google Patents
The preparation method of canna edulis ker polysaccharide polyphenol complex Download PDFInfo
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- CN101595991A CN101595991A CNA2009100543187A CN200910054318A CN101595991A CN 101595991 A CN101595991 A CN 101595991A CN A2009100543187 A CNA2009100543187 A CN A2009100543187A CN 200910054318 A CN200910054318 A CN 200910054318A CN 101595991 A CN101595991 A CN 101595991A
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Abstract
A kind of preparation method of canna edulis ker polysaccharide polyphenol complex of food technology field comprises the steps: to prepare canna edulis ker waste residue, adds the water mixing and obtains solution; The pH value of regulator solution is 7.5～8.5, adds thermally-stabilised AMS, makes diastatic mass fraction reach 0.5～1 ‰, 95～100 ℃ of following enzymolysis 30～60 minutes; The solution to 50 that cooling step two finally obtains～60 ℃ add alkaline papain, make the mass fraction of protease reach 1～2 ‰, enzymolysis 30～60 minutes; Add lipase afterwards, make its mass fraction reach 1～2 ‰, enzymolysis 30～60 minutes; The pH value of solution is adjusted to 9～11, and the adding volume fraction is 4～7% hydrogen peroxide, and 20～30 ℃ were stirred 30～60 minutes; Filter filter residue, washing is to neutral, drying is pulverized, and obtains canna edulis ker polysaccharide polyphenol complex.Raw material output of the present invention is big, and is cheap, and technology is simple, and cost is lower, and waste residue is recycled, and reduced environmental pollution.
Description
Technical field
The present invention relates to a kind of preparation method of food technology field, specifically is a kind of preparation method of canna edulis ker polysaccharide polyphenol complex.
Background technology
Functional bajiao banana taro dietary fiber is a kind of polysaccharide polyphenol complex.Polysaccharide in the bajiao banana taro dietary fiber is based on the natural macromolecular material of cellulose in the bajiao banana taro.Polysaccharide is the important component part of all living organisms, and required different physiological roles is relevant with earning a bare living.Polysaccharide be proved to be have strengthen body's immunity, antiviral, prevent and treat important physical functions such as diabetes, antitumor, anti-inflammatory, anticoagulation, radioresistance, reducing blood lipid.Polyphenol is the general name of polyhydric phenols in the bajiao banana taro.Plant polyphenol be proved to be have anti-oxidant, hemostasis and anti-inflammation, the disease-resistant bacterium of detoxifcation, anti-carious tooth, antiallergy, anti-sudden change, antitumor, anticancer, anti-nicotine, treatment cataract and angiocardiopathy, promotion immunity, delay senility, important physical functions such as sun-proof, skin whitening, moisturizing, crease-resistant and maintenance skin elasticity, be mainly used in health products such as antineoplastic, cardiovascular health medicine, anti-virus formulation, anti-diabetic and obesity, non-oxidizability food additives, antistaling agent, color preserving agent, deodorant etc.At present, along with the rise of the heat that goes back to nature, the plant polyose polyphenol is used by extensive studies.
Bajiao banana taro (Canna edulis Ker) main product is in provinces and regions, the west and south such as China Guizhou, Yunnan and Guangxi, its adaptability is strong, impoverishment tolerant, be easy to plantation, output is very big.At present, the bajiao banana taro mainly is used to produce starch and bean vermicelli.Canna edulis ker waste residue extracts remaining residue behind the starch for the bajiao banana taro, and these waste residue water content height are easy to fermentation, except that being processed on a small quantity the feed, the overwhelming majority is taken as refuse and throws away, and this has not only caused the huge waste of resource, and severe contamination local environment.Therefore recycle the added value that waste residue must improve canna edulis ker waste residue greatly, have good economic benefit, and alleviated environmental pollution, have the important social meaning.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of preparation method of canna edulis ker polysaccharide polyphenol complex is provided.Raw material output of the present invention is big, and is cheap, and technology is simple, and cost is lower, and waste residue is recycled, and reduced environmental pollution.
The present invention is achieved by the following technical solutions, the present invention relates to a kind of preparation method of canna edulis ker polysaccharide polyphenol complex, comprises the steps:
Step 1, the preparation canna edulis ker waste residue adds the water mixing and obtains solution;
Step 2, the pH value of regulator solution is 7.5～8.5, adds thermally-stabilised AMS, makes diastatic mass fraction reach 0.5～1 ‰, 95～100 ℃ of following enzymolysis 30～60 minutes;
Step 3, cooling solution to 50～60 ℃ add alkaline papain, make the mass fraction of protease reach 1～2 ‰, enzymolysis 30～60 minutes; Add lipase afterwards, make its mass fraction reach 1～2 ‰, enzymolysis 30～60 minutes;
Step 4 is adjusted to 9～11 with the pH value of solution, adds volume fraction and be 4～7% hydrogen peroxide, and 20～30 ℃ were stirred 30～60 minutes;
Step 5, filtering solution gets filter residue, and washing is to neutral, and drying under reduced pressure or vacuum freeze drying are pulverized, and obtain canna edulis ker polysaccharide polyphenol complex.
In the step 1, described preparation is specially: canna edulis ker waste residue in 50～60 ℃ of dryings, was pulverized 40 mesh sieves, crossed 160 mesh sieves afterwards, washed filtration with water under 25～40 ℃, filter residue.
In the step 1, describedly be mixed into ultrasonic processing 10～30 minutes.
In the step 1, described solution, the mass ratio of water and filter residue is (5～10) in the solution: 1.
In the step 5, described drying under reduced pressure is to carry out under 40～50 ℃.
The present invention has following beneficial effect: the present invention has carried out effective removal to starch, protein and fat, has improved extraction efficiency, has reduced the probability that chemical method destroys polyphenol; The present invention adopts hydrogen peroxide that dietary fiber is decoloured at normal temperatures, and employing low-temperature vacuum drying or freeze drying have reduced the destruction to polyphenol.Raw material output of the present invention is big, and is cheap, and technology is simple, and cost is lower, and waste residue is recycled, and reduced environmental pollution.The canna edulis ker polysaccharide polyphenol complex that the present invention obtains can be widely used in can be used as a kind of functional food additives simultaneously and being used for food in the health products such as fat-reducing, defaecation, toxin expelling, improves nutritive value, can also be used for cosmetics.
Description of drawings
Fig. 1 is preparation technology's flow chart of the present invention;
Fig. 2 is the solid of polysaccharide polyphenol complex
13The C-NMR wave spectrogram.
The specific embodiment
Following example will the invention will be further described in conjunction with the accompanying drawings.Present embodiment has provided detailed embodiment and process being to implement under the prerequisite with the technical solution of the present invention, but protection scope of the present invention is not limited to following embodiment.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.
As shown in Figure 1, the technology that the preparation technology's flow process of the embodiment in the specific embodiment shows in is with reference to the accompanying drawings implemented, and is specific as follows:
Embodiment 1
Step 1, canna edulis ker waste residue comes from Guizhou Zi Yunjia and Chemical Industry Science Co., Ltd, with canna edulis ker waste residue in 50 ℃ of dryings, pulverize, cross 40 mesh sieves, cross 160 mesh sieves afterwards to remove partial starch, residue in the collection screen, the water that adds 8 times of volumes, in 30 ℃ with filter residue agitator treating 20 minutes, cross the leaching filter residue with double gauze; Add the water of 8 times of filter residue volumes in the filter residue, ultrasonic processing 20 minutes obtains solution;
Step 2, the pH value of regulator solution is 8.2, adds thermally-stabilised AMS, makes diastatic mass fraction reach 0.6 ‰, 95 ℃ of following 800rpm stirred enzymolysis 60 minutes;
Step 3, the solution to 60 that cooling step two finally obtains ℃ adds alkaline papain, makes the mass fraction of protease reach 1 ‰, and 800rpm stirred enzymolysis 60 minutes; Add lipase afterwards, make its mass fraction reach 1 ‰, 800rpm stirs enzymolysis 60 minutes;
Step 4, the pH value of the solution that step 3 is finally obtained is adjusted to 11, adds volume fraction and be 4% hydrogen peroxide, and 25 ℃ were stirred 60 minutes;
Step 5, the solution that filtration step four finally obtains gets filter residue, and washing is to neutral, 40 ℃ of following drying under reduced pressure 24 hours are pulverized, and the filter residue that drying is good is pulverized 80 orders with common pulverizer, pulverize 300 orders with micronizer then, obtain canna edulis ker polysaccharide polyphenol complex.
The utilization solid
13C-NMR identifies the polysaccharide polyphenol complex that obtains, the results are shown in Figure 2.Wherein, chemical shift is that the signal of 172～174ppm is a carbonyl absorption peak, and chemical shift is that the multiplet of 127～155ppm is a double bond absorption peak, and this has shown the existence of polyphenol compound.Chemical shift is that 105ppm place sharp-pointed unimodal is the absworption peak of polysaccharide end group carbon, and chemical shift is that the multiplet of 54～72ppm is the absworption peak of all the other carbon of polysaccharide, and these peak-to-peak signals are stronger, shown the existence of a large amount of polysaccharide.
Embodiment 2
Step 1, canna edulis ker waste residue comes from Guizhou Zi Yunjia and Chemical Industry Science Co., Ltd, with canna edulis ker waste residue in 60 ℃ of dryings, pulverize, cross 40 mesh sieves, cross 160 mesh sieves afterwards to remove partial starch, residue in the collection screen, the water that adds 10 times of volumes, in 40 ℃ with filter residue agitator treating 10 minutes, cross the leaching filter residue with double gauze; Add the water of 10 times of filter residue volumes in the filter residue, ultrasonic processing 30 minutes obtains solution;
Step 2, the pH value of regulator solution is 8.0, adds thermally-stabilised alpha amylase, makes diastatic mass fraction reach 1 ‰, 100 ℃ of following 1200rpm stirred enzymolysis 30 minutes;
Step 3, the solution to 55 that cooling step two finally obtains ℃ adds alkaline papain, makes the mass fraction of protease reach 2 ‰, and 1200rpm stirred enzymolysis 30 minutes; Add lipase afterwards, make its mass fraction reach 2 ‰, 1200rpm stirred enzymolysis 30 minutes;
Step 4, the pH value of the solution that step 3 is finally obtained is adjusted to 9, adds volume fraction and be 7% hydrogen peroxide, and 30 ℃ were stirred 30 minutes;
Step 5, the solution that filtration step four finally obtains gets filter residue, and washing is to neutral, and vacuum freeze drying is pulverized, and the filter residue that drying is good is pulverized 100 orders with common pulverizer, pulverizes 400 orders with micronizer then, obtains canna edulis ker polysaccharide polyphenol complex.
Embodiment 3
Step 1, canna edulis ker waste residue comes from Guizhou Zi Yunjia and Chemical Industry Science Co., Ltd, with canna edulis ker waste residue in 55 ℃ of dryings, pulverize, cross 40 mesh sieves, cross 160 mesh sieves afterwards to remove partial starch, residue in the collection screen, the water that adds 8 times of volumes, in 25 ℃ with filter residue agitator treating 10 minutes, cross the leaching filter residue with double gauze; Add the water of 5 times of filter residue volumes in the filter residue, ultrasonic processing 15 minutes obtains solution;
Step 2, the pH value of regulator solution is 7.5, adds thermally-stabilised AMS, makes diastatic mass fraction reach 0.7 ‰, 96 ℃ of following enzymolysis 45 minutes;
Step 3, the solution to 50 that cooling step two finally obtains ℃ adds alkaline papain, makes the mass fraction of protease reach 1.5 ‰, enzymolysis 45 minutes; Add lipase afterwards, make its mass fraction reach 1.5 ‰, enzymolysis 45 minutes;
Step 4, the pH value of the solution that step 3 is finally obtained is adjusted to 10, adds volume fraction and be 6% hydrogen peroxide, and 20 ℃ were stirred 45 minutes;
Step 5, the solution that filtration step four finally obtains gets filter residue, and washing is to neutral, and 50 ℃ of drying under reduced pressure 20 hours are pulverized, and obtain canna edulis ker polysaccharide polyphenol complex.
Claims (5)
1, a kind of preparation method of canna edulis ker polysaccharide polyphenol complex is characterized in that, comprises the steps:
Step 1, the preparation canna edulis ker waste residue adds the water mixing and obtains solution;
Step 2, the pH value of regulator solution is 7.5～8.5, adds thermally-stabilised AMS, makes diastatic mass fraction reach 0.5～1 ‰, 95～100 ℃ of following enzymolysis 30～60 minutes;
Step 3, cooling solution to 50～60 ℃ add alkaline papain, make the mass fraction of protease reach 1～2 ‰, enzymolysis 30～60 minutes; Add lipase afterwards, make its mass fraction reach 1～2 ‰, enzymolysis 30～60 minutes;
Step 4 is adjusted to 9～11 with the pH value of solution, adds volume fraction and be 4～7% hydrogen peroxide, and 20～30 ℃ were stirred 30～60 minutes;
Step 5, filtering solution gets filter residue, and washing is to neutral, and drying under reduced pressure or vacuum freeze drying are pulverized, and obtain canna edulis ker polysaccharide polyphenol complex.
2, the preparation method of canna edulis ker polysaccharide polyphenol complex according to claim 1 is characterized in that, in the step 1, described preparation is specially: canna edulis ker waste residue in 50～60 ℃ of dryings, was pulverized 40 mesh sieves, cross 160 mesh sieves afterwards, wash filtration with water under 25～40 ℃, get filter residue.
3, the preparation method of canna edulis ker polysaccharide polyphenol complex according to claim 1 is characterized in that, in the step 1, describedly is mixed into ultrasonic processing 10～30 minutes.
4, the preparation method of canna edulis ker polysaccharide polyphenol complex according to claim 1 is characterized in that, in the step 1, and described solution, the mass ratio of water and filter residue is (5～10) in the solution: 1.
5, the preparation method of canna edulis ker polysaccharide polyphenol complex according to claim 1 is characterized in that, in the step 5, described drying under reduced pressure is to carry out under 40～50 ℃.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102161706A (en) * | 2011-03-01 | 2011-08-24 | 中国科学院过程工程研究所 | Comprehensive utilization method of Canna edulis Ker tuber |
| CN104292356A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Method for extracting peony polysaccharide from peony cakes with biological enzyme method |
| CN106723040A (en) * | 2016-12-27 | 2017-05-31 | 武汉轻工大学 | A kind of lotus root polyphenol polysaccharide-liposome complex liquid and preparation method thereof |
| CN108619001A (en) * | 2018-06-25 | 2018-10-09 | 上海交通大学医学院 | A kind of red ginseng facial mask and preparation method thereof of preservative free addition |
| CN109380711A (en) * | 2017-08-08 | 2019-02-26 | 贵州美人芋农业发展有限公司 | A kind of method that banana dasheen bar extracts plant sugar |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1318602C (en) * | 2004-12-22 | 2007-05-30 | 华南理工大学 | Method for preparing porous starch through enzyme method in high temperature |
| CN1300322C (en) * | 2005-05-26 | 2007-02-14 | 贵州大学 | Method for producing alcohol by using dry plate of banana dasheen as raw material |
-
2009
- 2009-07-02 CN CN2009100543187A patent/CN101595991B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102161706A (en) * | 2011-03-01 | 2011-08-24 | 中国科学院过程工程研究所 | Comprehensive utilization method of Canna edulis Ker tuber |
| CN102161706B (en) * | 2011-03-01 | 2013-04-10 | 中国科学院过程工程研究所 | Comprehensive utilization method of Canna edulis Ker tuber |
| CN104292356A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Method for extracting peony polysaccharide from peony cakes with biological enzyme method |
| CN106723040A (en) * | 2016-12-27 | 2017-05-31 | 武汉轻工大学 | A kind of lotus root polyphenol polysaccharide-liposome complex liquid and preparation method thereof |
| CN109380711A (en) * | 2017-08-08 | 2019-02-26 | 贵州美人芋农业发展有限公司 | A kind of method that banana dasheen bar extracts plant sugar |
| CN108619001A (en) * | 2018-06-25 | 2018-10-09 | 上海交通大学医学院 | A kind of red ginseng facial mask and preparation method thereof of preservative free addition |
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