CN102086209A

CN102086209A - Method for extracting sesamin from sesame cake meal

Info

Publication number: CN102086209A
Application number: CN2010105861371A
Authority: CN
Inventors: 刘勇; 汪维云
Original assignee: Individual
Current assignee: Individual
Priority date: 2010-12-14
Filing date: 2010-12-14
Publication date: 2011-06-08

Abstract

The invention discloses a method for extracting sesamin from sesame cake meal, wherein the sesamin is extracted from the raw material of waste sesame cake meal of sesame oil plants. The method comprises the steps of: removing residual oil by utilizing ethanol and ethyl acetate, hydrolyzing protein by utilizing protease, extracting the protein in the hydrolysate by utilizing alkali liquor, and subjecting the oil and protein removed sesame cake meal to supercritical CO2 extraction to obtain the sesamin. The method for extracting the sesamin from the sesame cake meal has the advantages that: physiological active materials such as the sesamin and the like with high additional value are extracted from the waste cake meal left after the sesame oil is extracted from the sesame, therefore, the environmental pollution caused by the waste sesame residue can be reduced and the good economic benefit can be produced so as to drive the further processing study of our country on the sesame, push forward the planting of a characteristic oil crop which is the sesame of our country, promote the structure adjustment of agriculture and increase the income of peasant.

Description

A kind of method of utilizing sesame cake meal to extract sesamin

Technical field:

The invention belongs to and relate to the method for extracting sesamin, especially a kind of method of from sesame cake meal, extracting sesamin.

Background technology:

Sesamin (sesamin) has another name called sesamin, sesamin, sesamin, is a kind of natural component in the til seed, and its content is about about 0.5%.122 ℃-123 ℃ of sesamin fusing point and solubleness, sesamin is dissolved in chloroform, ethanol and acetone arbitrarily.Pharmacological action is through the experiment invention of animal and human's body, effects such as sesamin has hypertension and cardiovascular hypertrophy, protects the liver, anti-oxidant, reducing cholesterol, inhibition skin carcinoma, protection liver.

Sesamin is because its distinctive physicochemical property and physiological function, in agricultural, food service industry and pharmaceutical industries great prospect, highly purified sesamin is applied in gradually and ensures and pharmaceutically, the price of highly purified sesamin is very expensive, this mainly is because sesamin presents stronger antioxygenation in vivo, have various physiological active functions, occurred adding the healthcare product of sesamin at present on the Japanese market.

China is sesame big producing country, and is general although sesame is had many uses, and is mainly used in the production of sesame oil.The production of domestic sesame oil generally employing machine is pressed method and water for method, the tankage that machine presses method are sesame cake meal, a domestic large-scale sesame oil factory can produce 1400 tons to 1500 tons in sesame oil every year, needs to consume 4000 tons sesame, and wherein the tankage of Chan Shenging are that grouts just have 2500 tons.At present, normally use as feed or fertilizer for these byproducts of grouts, utility value is very low, have in addition cause environmental pollution.Studies show that, produce the lignan compounds that still contains a great deal of in the sesame cake meal that produces behind the sesame oil, be i.e. the content of sesamin.If it is carried out deep processing Application and Development in addition, not only can realize the comprehensive utilization of waste material, and can also make additional value of farm products be ten times, tens times increase, produce considerable economic benefit.

The method of extracting sesamin at present from sesame cake meal has extracting method such as solvent-extraction process, solvent-recrystallization method, solvent-chromatography, but the problem that these methods exist is: the sesamin extraction yield is lower, and contain more grease, protein in the sesame cake meal, also extract and brought very big difficulty to sesamin, moreover because the chemical pollution in the solvent, cause quality low to product, shortcoming such as chemicals are residual.

Summary of the invention:

From sesame cake meal, extract the technological deficiency that sesamin exists at present in order to overcome, the present invention discloses a kind of method of utilizing sesame cake meal to extract sesamin, take supercritical extraction, whole process is not with an organic solvent, therefore there is not residual solvent in the extract sesamin, prevented that leaching process is to the murder by poisoning of human body with to the pollution of environment simultaneously yet.

The present invention is achieved through the following technical solutions its goal of the invention.

A kind of method of utilizing sesame cake meal to extract sesamin is a raw material with sesame oil factory waste grouts, extracts sesamin from sesame cake meal.At first utilize ethanol and ethyl acetate to slough remaining grease, use proteolytic enzyme protolysate again,, remove grease and proteinic sesame cake meal and pass through supercritical CO again with the protein in the alkali lye extraction hydrolyzed solution ₂Extraction promptly gets sesamin.

Its concrete steps are:

1, sesame cake meal degreasing: sesame cake meal is at first dry under 60-70 ℃ condition, be ground into 40 orders, carry out skimming treatment with 3 times of volume of ethanol and ethyl acetate again, degreasing 2 times, adopt 3000r/min centrifuging to carry out solid-liquid separation, remove the remaining grease in the sesame cake meal, drying and crushing gets sesame seed meal powder after the degreasing.

2, after degreasing, extract albumen the sesame seed meal powder: at first use trypsin hydrolyzing, handle 3h under 45-55 ℃, PH7.5, plum enzyme dosage 0.05% carries out alkali lye again and extracts, condition is that PH13, liquid material ratio are 12: 1, temperature is 75-85 ℃, and the time is 2H, adopts the centrifugal solid-liquid separation of carrying out of 3000r/min, solid phase is carried out drying and crushing, sesame seed meal behind the deproteinated, the liquid phase part concentrate drying gets albumen, can be used as fodder additives.

3, utilize the sesame seed meal powder behind the deproteinated to carry out supercritical CO ₂The extraction sesamin, the extraction process condition is: pressure 50Mpa, extraction temperature 50-55 ℃, extraction time 2h, CO ₂Flow 2L/min, entrainment agent are 85% ethanol, and flow is 0.2L/min.

Concrete technical process is as follows:

Sesame seed meal → drying → pulverizing → degreasing → solid-liquid separation → residue grease → other purposes

↓

Sesame seed meal after protease hydrolysis → pulverizing → drying → degreasing →

↓

Alkali lye separation → remaining protein → fodder additives

↓

Sesame seed meal entrainment agent behind the deproteinated

↓ ↓

Drying → pulverizing → supercritical extraction → sesamin

The invention has the beneficial effects as follows: utilize sesame to extract the physiologically active substance that waste grouts behind the sesame oil extract high added value such as sesamin and not only can reduce the environmental pollution that discarded sesame oil residue causes, can also produce good economic benefit, can drive the deep processing research of China's sesame, promote China characteristic oil crops---the plantation of sesame, promote agricultural restructuring, increase peasant's income.

Embodiment:

The invention will be further described below in conjunction with specific embodiment.

With 100 tons of sesame cake meals is raw material:

Carry out the sesame cake meal degreasing earlier: sesame cake meal is at first dry under 65 ℃ of conditions, be ground into 40 orders, carry out skimming treatment with 3 times of volume of ethanol and ethyl acetate again, degreasing 2 times, adopt 3000r/min centrifuging to carry out solid-liquid separation, remove the remaining grease in the sesame cake meal, drying and crushing gets sesame seed meal powder after the degreasing.

After degreasing, extract albumen again the sesame seed meal powder: at first use trypsin hydrolyzing, handle 3h under 50 ℃, PH7.5, plum enzyme dosage 0.05% carries out alkali lye again and extracts, condition is that PH13, liquid material ratio are 12: 1, temperature is 80 ℃, and the time is 2H, adopts the centrifugal solid-liquid separation of carrying out of 3000r/min, solid phase is carried out drying and crushing, sesame seed meal behind the deproteinated, the liquid phase part concentrate drying gets albumen, as fodder additives.

Utilize the sesame seed meal powder behind the deproteinated to carry out supercritical CO 2 extraction sesamin, extraction process at last: pressure 50Mpa, 50 ℃ of extraction temperature, extraction time 2h, CO ₂Flow 2L/min, entrainment agent are 85% ethanol, and flow is 0.2L/min.

Claims

1. method of utilizing sesame cake meal to extract sesamin, it is characterized in that: with sesame oil factory waste grouts is raw material, from sesame cake meal, extract sesamin, at first utilize ethanol and ethyl acetate to slough remaining grease, use proteolytic enzyme protolysate again, with the protein in the alkali lye extraction hydrolyzed solution, remove grease and proteinic sesame cake meal and pass through supercritical CO again ₂Extraction promptly gets sesamin.

2. a kind of method of utilizing sesame cake meal to extract sesamin according to claim 1, it is characterized in that: concrete steps are as follows:

(1) sesame cake meal degreasing: sesame cake meal is at first dry under 60-70 ℃ condition, be ground into 40 orders, carry out skimming treatment with 3 times of volume of ethanol and ethyl acetate again, degreasing 2 times, adopt 3000r/min centrifuging to carry out solid-liquid separation, remove the remaining grease in the sesame cake meal, drying and crushing gets sesame seed meal powder after the degreasing;

(2) after degreasing, extract albumen the sesame seed meal powder: at first use trypsin hydrolyzing, handle 3h under 45-55 ℃, PH7.5, plum enzyme dosage 0.05% carries out alkali lye again and extracts, condition is that PH13, liquid material ratio are 12: 1, temperature is 75-85 ℃, and the time is 2H, adopts the centrifugal solid-liquid separation of carrying out of 3000r/min, solid phase is carried out drying and crushing, sesame seed meal behind the deproteinated, the liquid phase part concentrate drying gets albumen, can be used as fodder additives;

(3) utilize the sesame seed meal powder behind the deproteinated to carry out supercritical CO ₂The extraction sesamin, the extraction process condition is: pressure 50Mpa, extraction temperature 50-55 ℃, extraction time 2h, CO ₂Flow 2L/min, entrainment agent are 85% ethanol, and flow is 0.2L/min.