CN109054992A - A method of extracting Seabuckthorm Seed Oil - Google Patents
A method of extracting Seabuckthorm Seed Oil Download PDFInfo
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- CN109054992A CN109054992A CN201811026971.8A CN201811026971A CN109054992A CN 109054992 A CN109054992 A CN 109054992A CN 201811026971 A CN201811026971 A CN 201811026971A CN 109054992 A CN109054992 A CN 109054992A
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- Prior art keywords
- seed oil
- seabuckthorm seed
- extracting
- extraction
- seabuckthorm
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
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- Extraction Or Liquid Replacement (AREA)
Abstract
The present invention provides a kind of method for extracting Seabuckthorm Seed Oil, comprising the following steps: and it is step 1, seabuckthorn seeds are dry, it crushed, fermented, obtain seabuckthorn seeds, obtained Seabuckthorm Seed Oil and extract raw material；Step 2, Seabuckthorm Seed Oil is extracted into raw material and extractant is put into the extraction kettle of subcritical fluid extraction device, extracted, obtain extract；Step 3, extract is separated, obtains Seabuckthorm Seed Oil.The present invention has carried out pre-processing to seabuckthorn seeds using fermentation method, fibr tissue and cell wall are destroyed by fermentation, to make the Seabuckthorm Seed Oil in seabuckthorn seeds be more easier to be extracted, so that the recovery rate of Seabuckthorm Seed Oil increases substantially, the yield and activity substance content of Seabuckthorm Seed Oil are improved.
Description
Technical field
The invention belongs to vegetable fat extractive technique fields, are related to a kind of method for extracting Seabuckthorm Seed Oil.
Background technique
Seabuckthorn seeds are mature kind of the dry behaviour of Elaeangnaceae hippophae plant sea-buckthorn (Hippophae rhamnoides L.)
Son.Qinghai-Tibet Platean of the sea-buckthorn originating from China, is mainly distributed on northwest China and the Northeast, is resistant to early anti-blown sand, and can be
It survives on salt-soda soil.Currently, domestic sea-buckthorn pulp and hippophae rhamnoides fruit oil have been fully utilized, a large amount of sea-buckthorn is left in processing
Seed.Seabuckthorn seeds are rich in various bioactivators, such as vitamin E, carotenoid, unsaturated fatty acid, yellow positive class, amino acid
And microelement etc., are penetrated by damage, cardio-cerebrovascular, immunity of organism etc. and is all had for anti-aging, anti-middle of the month tumor, anti-year richness
Apparent pharmacodynamics effect is one of modern medicine natural and precious yellow medicinal oil, and is widely used in food, changes
Cosmetic and medicines and health protection field.
The extraction of Seabuckthorm Seed Oil mainly uses Mechanical Method, organic solvent method and supercritical fluid extraction, Asia to face at present
Boundary's extraction.The mechanical pressing method squeezing time is long, oil yield is low, impurity content is high, oil quality is poor, and color is deeper；It is organic molten
Agent extraction is long, inefficient in the presence of asking when extracting, and solvent-oil ratio is big, and easily causes dissolvent residual etc. in product a series of
Drawback；And supercritical fluid extraction sets each requirement height to instrument, process flow is complicated, cost height, high production cost.It is subcritical
Fluid extraction technology (Subcritica1 fluid extraction techno1ogy) is to develop in recent years more quickly
Novel extractive technique, and a field technology revolution of extraction industry, have the advantages that product no solvent residue, while having again normal
Advise solvent extraction equipment low cost, the features such as process flow is simple, production cost is low, and entire extraction process it is closed absolutely
It is carried out under conditions of oxygen, low-temp low-pressure, the industrialization continuity particularly suitable for grease produces.But current sub-critical extraction
Rate and the content of active material are all lower.
Summary of the invention
Aiming at the problems existing in the prior art, the present invention provides a kind of method for extracting Seabuckthorm Seed Oil, Seabuckthorm Seed Oil
Yield and activity substance content improve.
The present invention is to be achieved through the following technical solutions:
A method of extracting Seabuckthorm Seed Oil, comprising the following steps:
Step 1, seabuckthorn seeds are dry, it crushed, fermented, obtain seabuckthorn seeds, obtained Seabuckthorm Seed Oil and extract raw material；
Step 2, Seabuckthorm Seed Oil is extracted into raw material and extractant is put into the extraction kettle of subcritical fluid extraction device, into
Row extraction, obtains extract；
Step 3, extract is separated, obtains Seabuckthorm Seed Oil.
Preferably, in step 1, the fermenting enzyme used that ferments is one of cellulase and pectase or two kinds of group
It closes.
Further, the dosage of fermenting enzyme is the 0.02~0.04% of seabuckthorn seeds quality.
Preferably, 40-55 DEG C of fermentation temperature, fermentation time 3-8h.
Preferably, in step 1, after fermentation, further include the steps that high pressure broken wall, high pressure 20Mp-60Mp.
Preferably, extractant is the mixture of propane or propane and dimethyl ether.
Preferably, in step 2, it is 1g:(1~15 that Seabuckthorm Seed Oil, which extracts raw material and the amount ratio of extractant) mL.
Preferably, in step 2, extraction temperature is 50~85 DEG C, and extracting pressure is 0.15~3MPa.
Preferably, in step 2, continuous extraction 1~20 time, 10~60min every time.
Preferably, in step 3, using decompression temperature-raising method, separate extract under the conditions of separation temperature is 10~60 DEG C
Out, Seabuckthorm Seed Oil is obtained.
Compared with prior art, the invention has the following beneficial technical effects:
The present invention has carried out pre-processing to seabuckthorn seeds using fermentation method, destroys fibr tissue and cell wall by fermentation,
To make the Seabuckthorm Seed Oil in seabuckthorn seeds be more easier to be extracted, so that the recovery rate of Seabuckthorm Seed Oil increases substantially, obtain
Oil cut rate reaches 11-14%；And the color of gained oil is brownish red, has the distinctive fragrance of seabuckthorn seeds, wherein carotenoid content
It is much higher than the resulting Seabuckthorm Seed Oil of other methods (respectively reaching 190mg/100g, 270mg/100g) with the content of vitamin E.This
Invention improves the yield of Seabuckthorm Seed Oil and the content of active material, is suitable for industrialized production.
Further, the present invention also optimizes Extraction solvent composition, is extracted using propane and dimethyl ether, dimethyl ether
Addition can increase permeability, effectively be extracted so that solvent is more easier to penetrate into seabuckthorn seeds, and dimethyl ether can change
The polarity of solvent is close to the polarity of Seabuckthorm Seed Oil, it is easier to the extraction of Seabuckthorm Seed Oil.
Further, it ferments and then is handled using 20Mp-60Mp high-pressure machinery broken wall method, further destroy cell
Wall promotes the extraction of Seabuckthorm Seed Oil.
Specific embodiment
Below with reference to specific embodiment, the present invention is described in further detail, it is described be explanation of the invention and
It is not to limit.
The method of the present invention for extracting Seabuckthorm Seed Oil is using subcritical fluid extraction method, comprising the following steps:
(1) resulting seed dries in the shade at room temperature after fresh, clean sea buckthorn fruit being squeezed the juice, after being crushed, being fermented
To treated seabuckthorn seeds, obtains seabuckthorn seeds and extract raw material；
(2) by treated the sea-buckthorn seed raw material with extractant by 1g:(1~15) material of mL is molten more subcritical than putting into
Continuous extraction 1~20 time in the extraction kettle of fluid extraction device, 10~60min, obtains extract every time；
(3) it using decompression temperature-raising method, separates the extract under the conditions of separation temperature is 10~60 DEG C, obtains seabuckthorn seeds
Oil.
The fermenting enzyme that fermentation in the step (1) uses is one of cellulase and pectase or two kinds of group
It closes；The dosage of fermenting enzyme is the 0.02~0.04% of seabuckthorn seeds quality；40-55 DEG C of fermentation temperature, fermentation time 3-8h.
In step (1), after fermentation, further include the steps that high pressure broken wall, high pressure 20Mp-60Mp.
It is 50~85 DEG C that extraction conditions, which is extraction temperature, in the step (2), and extracting pressure is 0.15~3MPa, is extracted molten
Agent ratio is 1:(1~15).
Specific embodiment is as follows.
Example 1
Seabuckthorn seeds after 100 kilograms are dried in the shade at room temperature spray the fruit of seabuckthorn seeds weight 0.01% through crushing while stirring
Glue enzyme and 0.01% cellulase, ferment at 40 DEG C 8h after mixing evenly, drying-granulating；Treated, and sea-buckthorn seed is pressed with propane
The material solute amount volume ratio (g/mL) of 1:5 is put into the extraction kettle of subcritical fluid extraction device, extraction temperature be 50 DEG C,
Extracting pressure is continuous extraction 5 times under conditions of 1Mpa, and each 30min obtains extract, and decompression separation obtains seabuckthorn seeds at 60 DEG C
11.6 kilograms of oil, oil yield 11.60%.
Example 2
Seabuckthorn seeds after 100 kilograms are dried in the shade at room temperature spray the fruit of seabuckthorn seeds weight 0.02% through crushing while stirring
Glue enzyme and 0.02% cellulase, ferment at 42 DEG C 7h after mixing evenly, drying-granulating；Treated sea-buckthorn seed and extractant
It is put into the extraction kettle of subcritical fluid extraction device by the material solute amount volume ratio (g/mL) of 1:10, extractant is by 1:1's
Propane and dimethyl ether composition, the continuous extraction 5 times under conditions of extraction temperature is 60 DEG C, extracting pressure is 1Mpa, every time
30min obtains extract, and decompression separation obtains 13.51 kilograms of Seabuckthorm Seed Oil at 60 DEG C, oil yield 13.51%.
Example 3
Seabuckthorn seeds after 100 kilograms are dried in the shade at room temperature spray the fruit of seabuckthorn seeds weight 0.01% through crushing while stirring
Glue enzyme and 0.01% cellulase, ferment at 45 DEG C 6h after mixing evenly, drying-granulating；Treated, and sea-buckthorn seed is pressed with propane
The material solute amount volume ratio (g/mL) of 1:10 is put into the extraction kettle of subcritical fluid extraction device, is 60 in extraction temperature
DEG C, extracting pressure be 1Mpa under conditions of continuous extraction 5 times, each 30min obtains extract, and decompression separation obtains husky at 40 DEG C
12.43 kilograms of oil of spine seed, oil yield 12.43%.
Example 4
Seabuckthorn seeds after 100 kilograms are dried in the shade at room temperature spray the fruit of seabuckthorn seeds weight 0.01% through crushing while stirring
Glue enzyme and 0.01% cellulase, ferment at 48 DEG C 5h, drying-granulating, then 20MPa high pressure broken wall after mixing evenly；Treated
Sea-buckthorn seed and extractant put into subcritical fluids chapter by the material solute amount volume ratio (g/mL) of 1:8 and the extraction of device are taken to take kettle
In, extractant is made of the propane and dimethyl ether of 1:1, continuous under conditions of extraction temperature is 60 DEG C, extracting pressure is 1Mpa
Extraction 5 times, each 30min, obtains extract, decompression separation obtains 14.01 kilograms of Seabuckthorm Seed Oil at 30 DEG C, oil yield 14.01%.
Example 5
Seabuckthorn seeds after 100 kilograms are dried in the shade at room temperature spray the fruit of seabuckthorn seeds weight 0.02% through crushing while stirring
Glue enzyme, ferment at 50 DEG C 4h, drying-granulating, then 30MPa high pressure broken wall after mixing evenly；Treated sea-buckthorn seed and extractant
Putting into subcritical fluids chapter by the material solute amount volume ratio (g/mL) of 1:1 takes the extraction of device to take in kettle, and extractant is by 1:1's
Propane and dimethyl ether composition, the continuous extraction 20 times under conditions of extraction temperature is 70 DEG C, extracting pressure is 0.15Mpa, every time
20min obtains extract, and decompression separation obtains 12.05 kilograms of Seabuckthorm Seed Oil at 20 DEG C, oil yield 14.05%.
Example 6
Seabuckthorn seeds after 100 kilograms are dried in the shade at room temperature spray 0.03% fiber of seabuckthorn seeds weight through crushing while stirring
Plain enzyme, ferment at 52 DEG C 4h, drying-granulating, then 40MPa high pressure broken wall after mixing evenly；Treated sea-buckthorn seed and extractant
Putting into subcritical fluids chapter by the material solute amount volume ratio (g/mL) of 1:12 takes the extraction of device to take in kettle, and extractant is by 1:1
Propane and dimethyl ether composition, the continuous extraction 10 times under conditions of extraction temperature is 80 DEG C, extracting pressure is 2Mpa, every time
10min obtains extract, and decompression separation obtains 13.22 kilograms of Seabuckthorm Seed Oil at 10 DEG C, oil yield 14.22%.
Example 7
Seabuckthorn seeds after 100 kilograms are dried in the shade at room temperature spray the fruit of seabuckthorn seeds weight 0.01% through crushing while stirring
Glue enzyme and 0.02% cellulase, ferment at 55 DEG C 3h, drying-granulating, then 60MPa high pressure broken wall after mixing evenly；Treated
Sea-buckthorn seed puts into subcritical fluids chapter by the material solute amount volume ratio (g/mL) of 1:15 with extractant and the extraction of device is taken to take
In kettle, extractant is made of the propane and dimethyl ether of 1:1, is connected under conditions of extraction temperature is 85 DEG C, extracting pressure is 3Mpa
Continuous extraction 1 time, time 60min, obtains extract, decompression separation obtains 13.68 kilograms of Seabuckthorm Seed Oil at 50 DEG C, oil yield
14.68%.
Using the content of carotenoid content and vitamin E in Seabuckthorm Seed Oil is obtained in standard method practical measuring examples 4, tie
Fruit shows that carotene carotene content reaches 190mg/100g, and the content of vitamin E reaches 270mg/100g, is much higher than other method institutes
The content of carrotene and vitamin E in the Seabuckthorm Seed Oil obtained.
The prior art is mainly adjusted Extracting temperature, time, pressure, solvent burden ratio, but effect promoting is unobvious,
The invention is characterized in that new material processing, improves the yield of sea-buckthorn and the content of active material.
Claims (10)
1. a kind of method for extracting Seabuckthorm Seed Oil, which comprises the following steps:
Step 1, seabuckthorn seeds are dry, it crushed, fermented, obtain seabuckthorn seeds, obtained Seabuckthorm Seed Oil and extract raw material；
Step 2, Seabuckthorm Seed Oil is extracted into raw material and extractant is put into the extraction kettle of subcritical fluid extraction device, extracted
It takes, obtains extract；
Step 3, extract is separated, obtains Seabuckthorm Seed Oil.
2. the method according to claim 1 for extracting Seabuckthorm Seed Oil, which is characterized in that in step 1, the fermentation for use of fermenting
Enzyme is one of cellulase and pectase or two kinds of combination.
3. the method according to claim 2 for extracting Seabuckthorm Seed Oil, which is characterized in that the dosage of fermenting enzyme is seabuckthorn seeds matter
The 0.02~0.04% of amount.
4. the method according to claim 1 for extracting Seabuckthorm Seed Oil, which is characterized in that 40-55 DEG C of fermentation temperature, when fermentation
Between be 3-8h.
5. the method according to claim 1 for extracting Seabuckthorm Seed Oil, which is characterized in that in step 1, after fermentation, also wrap
The step of including high pressure broken wall, high pressure 20Mp-60Mp.
6. the method according to claim 1 for extracting Seabuckthorm Seed Oil, which is characterized in that extractant be propane or propane with
The mixture of dimethyl ether.
7. the method according to claim 1 for extracting Seabuckthorm Seed Oil, which is characterized in that in step 2, Seabuckthorm Seed Oil extracts former
The amount ratio of material and extractant is 1g:(1~15) mL.
8. it is according to claim 1 extract Seabuckthorm Seed Oil method, which is characterized in that in step 2, extraction temperature be 50~
85 DEG C, extracting pressure is 0.15~3MPa.
9. the method according to claim 1 for extracting Seabuckthorm Seed Oil, which is characterized in that in step 2, continuous extraction 1~20
It is secondary, 10~60min every time.
10. the method according to claim 1 for extracting Seabuckthorm Seed Oil, which is characterized in that in step 3, heated up using decompression
Method separates extract under the conditions of separation temperature is 10~60 DEG C, obtains Seabuckthorm Seed Oil.
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| CN201811026971.8A CN109054992A (en) | 2018-09-04 | 2018-09-04 | A method of extracting Seabuckthorm Seed Oil |
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| CN201811026971.8A CN109054992A (en) | 2018-09-04 | 2018-09-04 | A method of extracting Seabuckthorm Seed Oil |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112029576A (en) * | 2020-08-26 | 2020-12-04 | 芜湖美町宝植物科技有限公司 | Supercritical carbon dioxide extraction method for sea buckthorn seed oil |
| CN112251284A (en) * | 2020-10-11 | 2021-01-22 | 甘肃省科学院生物研究所 | Method for extracting and refining sea-buckthorn whole fruit oil |
| CN112655776A (en) * | 2020-12-10 | 2021-04-16 | 四川饭扫光食品集团股份有限公司 | Preparation method and application of compound zanthoxylum oil |
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| CN102504939A (en) * | 2011-11-22 | 2012-06-20 | 中国科学院西北高原生物研究所 | Method for extracting sea buckthorn seed oil through subcritical fluid method |
| CN103242951A (en) * | 2013-05-06 | 2013-08-14 | 广西工学院 | Method for improving oil extraction pre-treatment technology |
| CN103981018A (en) * | 2014-04-23 | 2014-08-13 | 珀莱雅化妆品股份有限公司 | Preparation method of ganoderma lucidum spores oil |
| CN104178353A (en) * | 2014-09-04 | 2014-12-03 | 南京标科生物科技有限公司 | Process for extracting semen descurainiae volatile oil by adopting supercritical fluid method |
| CN106635400A (en) * | 2016-11-08 | 2017-05-10 | 新疆科宇科技有限公司 | Production method for extracting sea-buckthorn seed crude oil by adopting subcritical technology |
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2018
- 2018-09-04 CN CN201811026971.8A patent/CN109054992A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102504939A (en) * | 2011-11-22 | 2012-06-20 | 中国科学院西北高原生物研究所 | Method for extracting sea buckthorn seed oil through subcritical fluid method |
| CN103242951A (en) * | 2013-05-06 | 2013-08-14 | 广西工学院 | Method for improving oil extraction pre-treatment technology |
| CN103981018A (en) * | 2014-04-23 | 2014-08-13 | 珀莱雅化妆品股份有限公司 | Preparation method of ganoderma lucidum spores oil |
| CN104178353A (en) * | 2014-09-04 | 2014-12-03 | 南京标科生物科技有限公司 | Process for extracting semen descurainiae volatile oil by adopting supercritical fluid method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112029576A (en) * | 2020-08-26 | 2020-12-04 | 芜湖美町宝植物科技有限公司 | Supercritical carbon dioxide extraction method for sea buckthorn seed oil |
| CN112251284A (en) * | 2020-10-11 | 2021-01-22 | 甘肃省科学院生物研究所 | Method for extracting and refining sea-buckthorn whole fruit oil |
| CN112655776A (en) * | 2020-12-10 | 2021-04-16 | 四川饭扫光食品集团股份有限公司 | Preparation method and application of compound zanthoxylum oil |
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