CN103772521B - The extracting method of a kind of Dendrobium officinale polysaccharide - Google Patents
The extracting method of a kind of Dendrobium officinale polysaccharide Download PDFInfo
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 63
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 63
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 63
- 238000000034 method Methods 0.000 title claims abstract description 25
- 241001076416 Dendrobium tosaense Species 0.000 title claims abstract description 20
- 239000000243 solution Substances 0.000 claims abstract description 43
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 239000000284 extract Substances 0.000 claims abstract description 13
- 230000008014 freezing Effects 0.000 claims abstract description 9
- 238000007710 freezing Methods 0.000 claims abstract description 9
- 239000012670 alkaline solution Substances 0.000 claims abstract description 8
- 239000007787 solid Substances 0.000 claims abstract description 7
- 238000010257 thawing Methods 0.000 claims abstract description 7
- 239000003513 alkali Substances 0.000 claims abstract description 5
- 238000007654 immersion Methods 0.000 claims abstract description 5
- 239000003960 organic solvent Substances 0.000 claims abstract description 5
- 239000012467 final product Substances 0.000 claims abstract description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 19
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 7
- 238000000605 extraction Methods 0.000 abstract description 14
- 238000003913 materials processing Methods 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 241001523681 Dendrobium Species 0.000 description 14
- 238000001514 detection method Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 239000000203 mixture Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000001926 lymphatic effect Effects 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 229960000935 dehydrated alcohol Drugs 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 206010010144 Completed suicide Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 241000594394 Hedyotis Species 0.000 description 1
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
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- 235000006708 antioxidants Nutrition 0.000 description 1
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- 235000009508 confectionery Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
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- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 206010036067 polydipsia Diseases 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
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- 230000000630 rising effect Effects 0.000 description 1
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- 210000000434 stratum corneum Anatomy 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Abstract
The present invention relates to the extracting method of a kind of Dendrobium officinale polysaccharide, belong to Chinese medicinal materials processing technique field. It comprises the following steps: a, immersion: by fresh Herba Dendrobii, and chopping, is placed in-25 DEG C��-20 DEG C freezing 6��8 hours, after thawing, soaks with the organic solvent of 8��20 times of weight under normal temperature, removes soak solution, obtains the dregs of a decoction; B, alkali lye extract: by the described dregs of a decoction by solid liquid weight ratio be 1:10��40 add weight content be 0.5%��1% alkaline solution extract, obtain extracting solution; C, concentrated: extracting solution to be concentrated 1/2 to original volume, obtains concentrated solution; D, by described concentrated solution adjust ph to 9��11, leave standstill, filter, obtain filtered liquid I; E, described filtered liquid I is added gac, filter, obtain filtered liquid II, dry, to obtain final product. The invention has the beneficial effects as follows: the extraction yield that polysaccharide can be significantly improved, and polysaccharide content height in gained polyoses extract.
Description
Technical field
The present invention relates to Chinese medicinal materials processing technique field, it is specifically related to the extracting method of a kind of Dendrobium officinale polysaccharide.
Background technology
Herba Dendrobii is the stem of the orchid family Dendrobium plant Herba Dendrobii, has another name called Herba hedyotis costatae. The medicinal beginning is loaded in " Shennong Bencaojing ", is classified as top grade, has the empty labor of tonifying the five internal organs and wins thin, Qiang Yin, takes thick stomach for a long time and effect such as prolong life of making light of one's life by commiting suicide. Afterwards, history tree all has record. 2010 the version Pharmacopoeia of the People's Republic of China (one) it is single-row, load: taste is sweet, micro-cold. Return stomach, kidney channel. There is reinforcing stomach reg fluid, nourishing Yin and clearing heat effect. Hindering body fluid deficiency for the moon, dry polydipsia, deficiency of stomach-Yin, food is few retches, and abnormal heat is not moved back after being ill.
Finding through modern study, Herba Dendrobii contains the effective active compositions such as dendrobium polysaccharide, alkaloid, amino acid, luxuriant and rich with fragrance compounds, has the effects such as anti-oxidant, antitumor, reduction blood sugar, strengthening immunity, alleviating physical fatigue and clearing. In many activeconstituentss of Herba Dendrobii, dendrobium polysaccharide has very strong active. Therefore, the number of dendrobium polysaccharide content has become one of standard of judging Herba Dendrobii quality. Meanwhile, from Herba Dendrobii, extract polysaccharide component research and develop, also become a research focus.
Pertinent literature is reported, in Herba Dendrobii crude drug, the content of polysaccharide is generally 22%. In the method for existing extraction Dendrobium officinale polysaccharide, there is extraction yield not high, extract in polysaccharide the shortcomings such as foreign matter content height. Such as number of patent application is the Chinese invention patent " separation purification method of a kind of dendrobium polysaccharide " of 201110365323.7, adopt the chloroform of volume ratio 5:1: propyl carbinol mixed solution repetitive operation repeatedly deproteinated, while deproteination, also polysaccharide is stripped of, causes the reduction of polysaccharide extract rate. It also also exists the shortcoming such as complex production process, cost height.
Summary of the invention
It is an object of the invention to provide a kind of polysaccharide extract rate height, and the extracting method of the high Dendrobium officinale polysaccharide of polysaccharide content.
The technical solution used in the present invention is the extracting method of a kind of Dendrobium officinale polysaccharide, comprises the following steps:
A, immersion: by fresh Herba Dendrobii, chopping, is placed in-25 DEG C��-20 DEG C freezing 6��8 hours, after thawing, soaks with the organic solvent of 8��20 times of weight under normal temperature, removes soak solution, obtains the dregs of a decoction;
B, alkali lye extract: by the described dregs of a decoction by solid liquid weight ratio be 1:10��40 add weight content be 0.5%��1% alkaline solution extract, obtain extracting solution;
C, concentrated: extracting solution to be concentrated 1/2 to original volume, obtains concentrated solution;
D, by described concentrated solution adjust ph to 9��11, leave standstill, filter, obtain filtered liquid I;
E, described filtered liquid I is added gac, filter, obtain filtered liquid II, dry, to obtain final product.
Preferably, in described step a organic solvent be selected from dehydrated alcohol or mass concentration be more than 75% ethanolic soln in one, for the lipophilic composition of non-polyose is extracted.
As preferably, the one that in described step b, alkali is selected from sodium hydroxide or potassium hydroxide.
As preferably, the mode extracted in described step b adopts to soak or decoct and boils.
As preferably, the number of times extracted in described step b is 1��3 time, and the time of extraction is 2��6 hours.
As preferably, the time left standstill in described steps d is 24��48 hours.
As preferably, the weight that in described step e, gac adds is the 0.5%��1% of described filtered liquid I weight, for removing non-multi carbohydrate content better.
The present invention adopts fresh Herba Dendrobii, carry out freezing after chopping, have following effect: owing to Herba Dendrobii being rich in lymphatic temperament, adopt Herba Dendrobii after drying to extract polysaccharide, can there is non-polysaccharide component to be not easily extracted, cause the polysaccharide component after extracting to contain more impurity. Studying discovery through contriver, the lymphatic temperament in Herba Dendrobii, when Herba Dendrobii is dry, can wrap up the composition in Herba Dendrobii so that it is be not easy stripping, and Herba Dendrobii epidermis also can form stratum corneum, and the composition in Herba Dendrobii also can be made to be not easy stripping. Therefore, the present invention directly adopts fresh Herba Dendrobii, and makes full use of the characteristic of Herba Dendrobii, take to shred and freezing technique, it is possible to allow the moisture in Herba Dendrobii cell form ice crystal, and puncture cell, avoid lymphatic temperament parcel composition, the not easily phenomenon of stripping.
In the present invention, it is preferable that weight content is the alkaline solution extraction dendrobium polysaccharide of 0.5%��1%, can effectively extract polysaccharide, and can also remove the impurity such as protein. Find after deliberation, when the weight content of alkaline solution is 0.5%��1%, the extraction yield of dendrobium polysaccharide can be allowed the highest. When the alkaline solution lower than 0.5% or the alkaline solution higher than 1%, the extraction yield of polysaccharide is about 25%. When extracting with the alkaline solution of 0.5%��1%, the extraction yield of polysaccharide has remarkable rising, achieves good technique effect.
Extracting solution is concentrated by the present invention, concentrates and normal pressure mode that is concentrated or concentrating under reduced pressure can be adopted to carry out.
In the present invention, by described concentrated solution adjust ph to 9��11, then leave standstill, for removing the non-multi carbohydrate contents such as protein better.
The useful effect of the present invention is:
(1) extracting method of the present invention, can effectively improve the extraction yield of dendrobium polysaccharide, can reach more than 36%. After testing, in gained dendrobium polysaccharide, the content of dendrobium polysaccharide is more than 96%, and non-polysaccharide component content is few. Compare the method that existing water extract-alcohol precipitation extracts Dendrobium officinale polysaccharide, the extraction yield of polysaccharide increases by more than 5%, non-polysaccharide constituent reduction about 30% in gained Dendrobium officinale polysaccharide.
(2) extracting method of the present invention, scientific and reasonable, easy and simple to handle, is easy to the big production of mass-producing.
Embodiment
For making those skilled in the art understand production technique and the technique effect of the present invention in detail, introduce application and the technique effect of the present invention below further with concrete production instance.
Embodiment one:
Getting fresh Herba Dendrobii, chopping, is placed in-25 DEG C freezing 7 hours, after thawing, soaks with the dehydrated alcohol of 10 times of weight under normal temperature, removes soak solution, obtains the dregs of a decoction; By the dregs of a decoction by solid liquid weight ratio be 1:20 add weight content be 0.5% sodium hydroxide solution carry out decocting and boil, decocting the number of times that boils is 3 times, and decoct the time boiled is 4 hours every time, merges each extracting solution, extracting solution normal pressure is concentrated 1/2 to original volume, obtains concentrated solution; By described concentrated solution adjust ph to 10, leave standstill 48 hours, filter, obtain filtered liquid I; Described filtered liquid I is added gac, filters, obtain filtered liquid II, dry, obtain Dendrobium officinale polysaccharide. Wherein, the weight that gac adds is the 1% of described filtered liquid I weight.
After testing, the extraction yield of dendrobium polysaccharide is 37%, and the content of its polysaccharide is 97%. The detection of its polysaccharide content carries out according to polysaccharide content detection method under version " Chinese Pharmacopoeia " first Herba Dendrobii item in 2010.
Embodiment two:
Getting fresh Herba Dendrobii, chopping, is placed in-20 DEG C freezing 8 hours, and after thawing under normal temperature, the ethanolic soln being 75% by the mass concentration of 20 times of weight soaks, and removes soak solution, obtains the dregs of a decoction; By the dregs of a decoction by solid liquid weight ratio be 1:40 add weight content be 0.5% sodium hydroxide solution carry out decocting and boil, decocting the number of times that boils is 1 time, and decoct the time boiled is 6 hours every time, obtains extracting solution, extracting solution is evaporated to the 1/2 of original volume, obtains concentrated solution; By concentrated solution adjust ph to 11, leave standstill 24 hours, filter, obtain filtered liquid I; Filtered liquid I is added gac, filters, obtain filtered liquid II, dry, obtain Dendrobium officinale polysaccharide. Wherein, the weight that gac adds is the 0.5% of described filtered liquid I weight.
After testing, the extraction yield of dendrobium polysaccharide is 37%, and the content of its polysaccharide is 96%. The detection of its polysaccharide content carries out according to polysaccharide content detection method under version " Chinese Pharmacopoeia " first Herba Dendrobii item in 2010.
Embodiment three:
Getting fresh Herba Dendrobii, chopping, is placed in-22 DEG C freezing 6 hours, and after thawing under normal temperature, the ethanolic soln being 95% by the mass concentration of 8 times of weight soaks, and removes soak solution, obtains the dregs of a decoction; By the dregs of a decoction by solid liquid weight ratio be 1:10 add weight content be 0.5% potassium hydroxide solution soak, the number of times of immersion is 3 times, and the time every time soaked is 2 hours, merge each soak solution, obtain extracting solution, extracting solution is evaporated to the 1/2 of original volume, obtains concentrated solution; By concentrated solution adjust ph to 9, leave standstill 36 hours, filter, obtain filtered liquid I; Filtered liquid I is added gac, filters, obtain filtered liquid II, dry, obtain Dendrobium officinale polysaccharide. Wherein, the weight that gac adds is the 0.8% of described filtered liquid I weight.
After testing, the extraction yield of dendrobium polysaccharide is 36%, and the content of its polysaccharide is 97%. The detection of its polysaccharide content carries out according to polysaccharide content detection method under version " Chinese Pharmacopoeia " first Herba Dendrobii item in 2010.
Embodiment four:
Getting fresh Herba Dendrobii, chopping, is placed in-24 DEG C freezing 8 hours, and after thawing under normal temperature, the ethanolic soln being 85% by the mass concentration of 8 times of weight soaks, and removes soak solution, obtains the dregs of a decoction; By the dregs of a decoction by solid liquid weight ratio be 1:30 add weight content be 0.5% sodium hydroxide solution soak, the number of times of immersion is 3 times, and the time every time soaked is 4 hours, merge each soak solution, obtain extracting solution, extracting solution is evaporated to the 1/2 of original volume, obtains concentrated solution; By concentrated solution adjust ph to 10, leave standstill 36 hours, filter, obtain filtered liquid I; Filtered liquid I is added gac, filters, obtain filtered liquid II, dry, obtain Dendrobium officinale polysaccharide. Wherein, the weight that gac adds is the 0.8% of described filtered liquid I weight.
After testing, the extraction yield of dendrobium polysaccharide is 38%, and the content of its polysaccharide is 97%. The detection of its polysaccharide content carries out according to polysaccharide content detection method under version " Chinese Pharmacopoeia " first Herba Dendrobii item in 2010.
Finally it should be noted that, the technical scheme of above embodiment only unrestricted the present invention in order to explanation, although with reference to above-described embodiment to invention has been detailed explanation, those skilled in the art are to be understood that, still the present invention can be modified or equivalent replacement, and not departing from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in the right of the present invention.
Claims (7)
1. the extracting method of a Dendrobium officinale polysaccharide, it is characterised in that: comprise the following steps:
A, immersion: by fresh Herba Dendrobii, chopping, is placed in-25 DEG C��-20 DEG C freezing 6��8 hours, after thawing, soaks with the organic solvent of 8��20 times of weight under normal temperature, removes soak solution, obtains the dregs of a decoction;
B, alkali lye extract: by the described dregs of a decoction by solid liquid weight ratio be 1:10��40 add weight content be 0.5%��1% alkaline solution extract, obtain extracting solution;
C, concentrated: extracting solution to be concentrated 1/2 to original volume, obtains concentrated solution;
D, by described concentrated solution adjust ph to 9��11, leave standstill, filter, obtain filtered liquid I;
E, described filtered liquid I is added gac, filter, obtain filtered liquid II, dry, to obtain final product.
2. the extracting method of a kind of Dendrobium officinale polysaccharide according to claim 1, it is characterised in that: in described step a, organic solvent is selected from the ethanolic soln that mass concentration is more than 75%.
3. the extracting method of a kind of Dendrobium officinale polysaccharide according to claim 1 and 2, it is characterised in that: the one that in described step b, alkali is selected from sodium hydroxide or potassium hydroxide.
4. the extracting method of a kind of Dendrobium officinale polysaccharide according to claim 3, it is characterised in that: the mode extracted in described step b adopts to soak or decoct and boils.
5. the extracting method of a kind of Dendrobium officinale polysaccharide according to claim 4, it is characterised in that: the number of times extracted in described step b is 1��3 time, and the time every time extracted is 2��6 hours.
6. the extracting method of a kind of Dendrobium officinale polysaccharide according to claim 5, it is characterised in that: the time left standstill in described steps d is 24��48 hours.
7. the extracting method of a kind of Dendrobium officinale polysaccharide according to claim 6, it is characterised in that: the weight that in described step e, gac adds is the 0.5%��1% of described filtered liquid I weight.
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| CN104892783A (en) * | 2015-05-11 | 2015-09-09 | 南昌大学 | Purification method of dendrobium officinale polysaccharide |
| CN104844723B (en) * | 2015-06-05 | 2016-10-26 | 云南金九地生物科技有限公司 | A kind of preparation method and application of Herba Dendrobii extract |
| CN106692692A (en) * | 2015-08-21 | 2017-05-24 | 中国科学院昆明植物研究所 | Dendrobium officinale extract and preparation method thereof |
| CN106674364A (en) * | 2015-11-06 | 2017-05-17 | 广西南宁市邕王医药科技有限公司 | Extracting method for dendrobium polysaccharide |
| CN106117385A (en) * | 2016-06-28 | 2016-11-16 | 桐城市雨润生物科技有限公司 | A kind of extraction process of Herba Dendrobii tissue cultured seedling polysaccharide |
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| CN107686526A (en) * | 2017-09-29 | 2018-02-13 | 南京仙草堂生物科技有限公司 | A kind of extraction of dendrobium candidum root dendrobium polysaccharide and purifying process |
| CN107974475A (en) * | 2017-12-29 | 2018-05-01 | 贺州市星辉科技有限公司 | A kind of technique of enzyme edman degradation Edman from dendrobium candidum leaf extraction polysaccharide |
| CN107936131A (en) * | 2017-12-29 | 2018-04-20 | 贺州市星辉科技有限公司 | A kind of technique of acid-base method extraction Dendrobium officinale polysaccharide |
| CN107880149A (en) * | 2017-12-29 | 2018-04-06 | 贺州市星辉科技有限公司 | A kind of extraction process of Dendrobium officinale polysaccharide |
| CN108794650A (en) * | 2018-08-20 | 2018-11-13 | 云南和瑾科技有限公司 | A kind of dendrobium polysaccharide extracting method |
| CN110478436A (en) * | 2019-08-21 | 2019-11-22 | 四川省峰上生物科技有限公司 | A kind of preparation method of dendrobium candidum extract |
| CN111171173A (en) * | 2020-02-19 | 2020-05-19 | 杭州师范大学 | Method for quickly and efficiently extracting dendrobium officinale polysaccharide |
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