CN103772521A - Extracting method of dendrobium officinale polysaccharides - Google Patents
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 65
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 65
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 65
- 238000000034 method Methods 0.000 title claims abstract description 26
- 241001076416 Dendrobium tosaense Species 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 239000000284 extract Substances 0.000 claims abstract description 15
- 230000008014 freezing Effects 0.000 claims abstract description 8
- 238000007710 freezing Methods 0.000 claims abstract description 8
- 239000003513 alkali Substances 0.000 claims abstract description 6
- 239000003960 organic solvent Substances 0.000 claims abstract description 5
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 38
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 19
- 238000000605 extraction Methods 0.000 claims description 14
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 7
- 239000012670 alkaline solution Substances 0.000 claims description 7
- 230000001105 regulatory effect Effects 0.000 claims description 7
- 238000010257 thawing Methods 0.000 claims description 6
- 238000007654 immersion Methods 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 3
- 239000012467 final product Substances 0.000 claims description 2
- 238000002791 soaking Methods 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 241000411851 herbal medicine Species 0.000 abstract description 2
- 239000000706 filtrate Substances 0.000 abstract 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 2
- 238000001035 drying Methods 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 241001523681 Dendrobium Species 0.000 description 14
- 238000001514 detection method Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 230000001926 lymphatic effect Effects 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000003014 reinforcing effect Effects 0.000 description 2
- 206010010144 Completed suicide Diseases 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 241000594394 Hedyotis Species 0.000 description 1
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000003913 materials processing Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 206010036067 polydipsia Diseases 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000000434 stratum corneum Anatomy 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to an extracting method of dendrobium officinale polysaccharides, and belongs to the technical field of processing of Chinese herbal medicines. The extracting method comprises the following steps of: a, soaking, namely, cutting up fresh dendrobium officinale, freezing for 6 to 8 hours under a temperature of -25 to -20 DEG C, then unfreezing under normal temperature, soaking with an organic solvent which is 8 to 20 times by weight, and then removing the soaking liquid to obtain the residues; b, extracting with alkali liquor, namely, adding 0.5 to 1wt% alkali liquor to the residues based on the weight ratio of solid to liquor of 1: 10 to 40 for extracting to obtain extract liquor; c, concentrating, namely, concentrating the extract liquor to be 1/2 of the original volume, thus obtaining concentrated liquor; d, adjusting pH of the concentrated liquor to be 9 to 11, standing, and filtering to obtain the filtrate I; e, adding activated carbon to the filtrate I, filtering to obtain filtrate II, and then drying to obtain dendrobium officinale polysaccharides. The extracting method has the beneficial effects that the extracting rate of polysaccharides is obviously increased, and the obtained polysaccharides extract is high in polysaccharides content.
Description
Technical field
The present invention relates to Chinese medicinal materials processing technique field, be specifically related to a kind of extracting method of Dendrobium officinale polysaccharide.
Background technology
Herba Dendrobii is the stem of the orchid family Dendrobium Sw Herba Dendrobii, has another name called Herba hedyotis costatae.The medicinal beginning is loaded in Shennong's Herbal, is classified as top gradely, has tonifying the five internal organs consumptive disease and wins thinly, and reinforcing yin essence, takes for a long time thick stomach and make light of one's life by commiting suicide the effect such as prolong life.Afterwards, successive dynasties book on Chinese herbal medicine is all on the books.2010 the version Pharmacopoeia of the People's Republic of China (one) it is single-row, carry: taste is sweet, is slightly cold.Return stomach, kidney channel.There is reinforcing stomach reg fluid, nourishing Yin and clearing heat effect.Hinder body fluid deficiency for the moon, dry polydipsia, deficiency of stomach-Yin, food is few retches, and abnormal heat is not moved back etc. after being ill.
Find through modern study, Herba Dendrobii contains the effective active compositions such as dendrobium polysaccharide, alkaloid, amino acid, luxuriant and rich with fragrance compounds, have anti-oxidant, antitumor, reduce the effects such as blood sugar, strengthening immunity, alleviating physical fatigue and clearing.In many activeconstituentss of Herba Dendrobii, dendrobium polysaccharide has very strong active.Therefore, the number of dendrobium polysaccharide content has become one of standard judging Herba Dendrobii quality.Meanwhile, from Herba Dendrobii, extract polysaccharide component and research and develop, also become a study hotspot.
Pertinent literature report, in Herba Dendrobii crude drug, the content of polysaccharide is generally 22%.In the method for existing extraction Dendrobium officinale polysaccharide, exist extraction yield not high, extract in polysaccharide the shortcomings such as foreign matter content height.The Chinese invention patent that is 201110365323.7 such as number of patent application " a kind of separation purification method of dendrobium polysaccharide ", adopt the chloroform of volume ratio 5:1: repeatedly deproteinated of propyl carbinol mixed solution repetitive operation, in deproteination, also polysaccharide has been removed, caused the reduction of polysaccharide extract rate.It also exists complex production process, high in cost of production shortcoming.
Summary of the invention
The object of this invention is to provide a kind of polysaccharide extract rate high, and the extracting method of the high Dendrobium officinale polysaccharide of polysaccharide content.
The technical solution used in the present invention is a kind of extracting method of Dendrobium officinale polysaccharide, comprises the following steps:
A, immersion: by fresh Herba Dendrobii, chopping, be placed in-25 ℃～-20 ℃ freezing 6～8 hours, after thawing under normal temperature, soak with the organic solvent of 8～20 times of weight, removal soak solution, obtains the dregs of a decoction;
B, alkali lye extract: be that to add weight content be that 0.5%～1% alkaline solution extracts in 1:10～40 by the described dregs of a decoction by solid-liquid weight ratio, obtain extracting solution;
C, concentrated: extracting solution is concentrated into 1/2 of original volume, obtains concentrated solution;
D, described concentrated solution is regulated to pH value to 9～11, leave standstill, filtration, obtains filtered liquid I;
E, described filtered liquid I is added to gac, filter, obtain filtered liquid II, dry, to obtain final product.
Preferably, in described step a, organic solvent is selected from dehydrated alcohol or mass concentration is the one in more than 75% ethanolic soln, for the lipophilic composition of non-polyose is extracted.
As preferably, in described step b, alkali is selected from the one in sodium hydroxide or potassium hydroxide.
As preferably, the mode of extracting in described step b adopts soaks or decocts.
As preferably, the number of times extracting in described step b is 1～3 time, and the time of extraction is 2～6 hours.
As preferably, in described steps d, the standing time is 24～48 hours.
As preferably, the weight that in described step e, gac adds is 0.5%～1% of described filtered liquid I weight, for removing better non-polyose composition.
In the present invention, adopt fresh Herba Dendrobii, after chopping, carry out freezingly, have following effect: owing to being rich in lymphatic temperament in Herba Dendrobii, adopt dry after Herba Dendrobii extract polysaccharide, can exist non-polysaccharide component to be difficult for being extracted out, cause the polysaccharide component after extracting to contain more impurity.Study discovery through contriver, the lymphatic temperament in Herba Dendrobii, in the time that Herba Dendrobii is dry, can wrap up the composition in Herba Dendrobii, makes it be not easy stripping, and Herba Dendrobii epidermis also can form stratum corneum, also can make the composition in Herba Dendrobii be not easy stripping.Therefore, the present invention directly adopts fresh Herba Dendrobii, and makes full use of the characteristic of Herba Dendrobii, take chopping and freezing technique, can allow the moisture in Herba Dendrobii cell form ice crystal, and sting broken cell, avoid lymphatic temperament parcel composition, be difficult for the phenomenon of stripping.
In the present invention, the alkaline solution that preferred weight content is 0.5%～1% extracts dendrobium polysaccharide, can effectively extract polysaccharide, and can also remove the impurity such as protein.Find after deliberation, when the weight content of alkaline solution is 0.5%～1%, can allow the extraction yield of dendrobium polysaccharide the highest.Alkaline solution lower than 0.5% or during higher than 1% alkaline solution, the extraction yield of polysaccharide is 25% left and right.While extraction with 0.5%～1% alkaline solution, the extraction yield of polysaccharide has remarkable rising, has obtained good technique effect.
In the present invention, extracting solution is concentrated, the concentrated normal pressure mode concentrated or concentrating under reduced pressure that can adopt is carried out.
In the present invention, described concentrated solution is regulated to pH value to 9～11, then leave standstill, for removing better the non-polyose compositions such as protein.
Beneficial effect of the present invention is:
(1) extracting method of the present invention, can effectively improve the extraction yield of dendrobium polysaccharide, can reach more than 36%.After testing, in gained dendrobium polysaccharide, the content of dendrobium polysaccharide is more than 96%, and non-polysaccharide component content is few.Compare the method that existing water extract-alcohol precipitation extracts Dendrobium officinale polysaccharide, the extraction yield of polysaccharide increases more than 5%, and in gained Dendrobium officinale polysaccharide, non-polysaccharide component has reduced by 30% left and right.
(2) extracting method of the present invention, scientific and reasonable, easy and simple to handle, is easy to the large production of mass-producing.
Embodiment
For making those skilled in the art understand in detail production technique of the present invention and technique effect, further introduce application of the present invention and technique effect with concrete production instance below.
Embodiment mono-:
Get fresh Herba Dendrobii, chopping, be placed in-25 ℃ freezing 7 hours, after thawing under normal temperature, soak with the dehydrated alcohol of 10 times of weight, removal soak solution, obtains the dregs of a decoction; By the dregs of a decoction by solid-liquid weight ratio be 1:20 to add weight content be that 0.5% sodium hydroxide solution decocts, the number of times of decoction is 3 times, each time decocting is 4 hours, merges extracting solution each time, and extracting solution normal pressure is concentrated into 1/2 of original volume, obtains concentrated solution; Described concentrated solution is regulated to pH value to 10, leave standstill 48 hours, filter, obtain filtered liquid I; Described filtered liquid I is added to gac, filter, obtain filtered liquid II, dry, obtain Dendrobium officinale polysaccharide.Wherein, the weight that gac adds is 1% of described filtered liquid I weight.
After testing, the extraction yield of dendrobium polysaccharide is 37%, and the content of its polysaccharide is 97%.The detection of its polysaccharide content is carried out according to polysaccharide content detection method under version " Chinese Pharmacopoeia " First Herba Dendrobii item in 2010.
Embodiment bis-:
Get fresh Herba Dendrobii, chopping, be placed in-20 ℃ freezing 8 hours, after thawing under normal temperature, soak with the ethanolic soln that the mass concentration of 20 times of weight is 75%, removal soak solution, obtains the dregs of a decoction; By the dregs of a decoction by solid-liquid weight ratio be 1:40 to add weight content be that 0.5% sodium hydroxide solution decocts, the number of times of decoction is 1 time, each time decocting is 6 hours, obtains extracting solution, and extracting solution is evaporated to 1/2 of original volume, obtains concentrated solution; Concentrated solution is regulated to pH value to 11, leave standstill 24 hours, filter, obtain filtered liquid I; Filtered liquid I is added to gac, filter, obtain filtered liquid II, dry, obtain Dendrobium officinale polysaccharide.Wherein, the weight that gac adds is 0.5% of described filtered liquid I weight.
After testing, the extraction yield of dendrobium polysaccharide is 37%, and the content of its polysaccharide is 96%.The detection of its polysaccharide content is carried out according to polysaccharide content detection method under version " Chinese Pharmacopoeia " First Herba Dendrobii item in 2010.
Embodiment tri-:
Get fresh Herba Dendrobii, chopping, be placed in-22 ℃ freezing 6 hours, after thawing under normal temperature, soak with the ethanolic soln that the mass concentration of 8 times of weight is 95%, removal soak solution, obtains the dregs of a decoction; By the dregs of a decoction by solid-liquid weight ratio be 1:10 to add weight content be that 0.5% potassium hydroxide solution soaks, the number of times of immersion is 3 times, each time of soaking is 2 hours, merge soak solution each time, obtain extracting solution, extracting solution is evaporated to 1/2 of original volume, obtain concentrated solution; Concentrated solution is regulated to pH value to 9, leave standstill 36 hours, filter, obtain filtered liquid I; Filtered liquid I is added to gac, filter, obtain filtered liquid II, dry, obtain Dendrobium officinale polysaccharide.Wherein, the weight that gac adds is 0.8% of described filtered liquid I weight.
After testing, the extraction yield of dendrobium polysaccharide is 36%, and the content of its polysaccharide is 97%.The detection of its polysaccharide content is carried out according to polysaccharide content detection method under version " Chinese Pharmacopoeia " First Herba Dendrobii item in 2010.
Embodiment tetra-:
Get fresh Herba Dendrobii, chopping, be placed in-24 ℃ freezing 8 hours, after thawing under normal temperature, soak with the ethanolic soln that the mass concentration of 8 times of weight is 85%, removal soak solution, obtains the dregs of a decoction; By the dregs of a decoction by solid-liquid weight ratio be 1:30 to add weight content be that 0.5% sodium hydroxide solution soaks, the number of times of immersion is 3 times, each time of soaking is 4 hours, merge soak solution each time, obtain extracting solution, extracting solution is evaporated to 1/2 of original volume, obtain concentrated solution; Concentrated solution is regulated to pH value to 10, leave standstill 36 hours, filter, obtain filtered liquid I; Filtered liquid I is added to gac, filter, obtain filtered liquid II, dry, obtain Dendrobium officinale polysaccharide.Wherein, the weight that gac adds is 0.8% of described filtered liquid I weight.
After testing, the extraction yield of dendrobium polysaccharide is 38%, and the content of its polysaccharide is 97%.The detection of its polysaccharide content is carried out according to polysaccharide content detection method under version " Chinese Pharmacopoeia " First Herba Dendrobii item in 2010.
Finally it should be noted that, above embodiment is the unrestricted technical scheme of the present invention in order to explanation only, although the present invention is had been described in detail with reference to above-described embodiment, those skilled in the art are to be understood that, still can modify or be equal to replacement the present invention, and not departing from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in claim scope of the present invention.
Claims (7)
1. an extracting method for Dendrobium officinale polysaccharide, is characterized in that: comprise the following steps:
A, immersion: by fresh Herba Dendrobii, chopping, be placed in-25 ℃～-20 ℃ freezing 6～8 hours, after thawing under normal temperature, soak with the organic solvent of 8～20 times of weight, removal soak solution, obtains the dregs of a decoction;
B, alkali lye extract: be that to add weight content be that 0.5%～1% alkaline solution extracts in 1:10～40 by the described dregs of a decoction by solid-liquid weight ratio, obtain extracting solution;
C, concentrated: extracting solution is concentrated into 1/2 of original volume, obtains concentrated solution;
D, described concentrated solution is regulated to pH value to 9～11, leave standstill, filtration, obtains filtered liquid I;
E, described filtered liquid I is added to gac, filter, obtain filtered liquid II, dry, to obtain final product.
2. the extracting method of a kind of Dendrobium officinale polysaccharide according to claim 1, is characterized in that: in described step a, organic solvent is selected from dehydrated alcohol or mass concentration is the one in more than 75% ethanolic soln.
3. the extracting method of a kind of Dendrobium officinale polysaccharide according to claim 1 and 2, is characterized in that: in described step b, alkali is selected from the one in sodium hydroxide or potassium hydroxide.
4. the extracting method of a kind of Dendrobium officinale polysaccharide according to claim 3, is characterized in that: the mode of extracting in described step b adopts soaks or decocts.
5. the extracting method of a kind of Dendrobium officinale polysaccharide according to claim 4, is characterized in that: the number of times extracting in described step b is 1～3 time, and the time of extraction is 2～6 hours.
6. the extracting method of a kind of Dendrobium officinale polysaccharide according to claim 5, is characterized in that: in described steps d, the standing time is 24～48 hours.
7. the extracting method of a kind of Dendrobium officinale polysaccharide according to claim 6, is characterized in that: the weight that in described step e, gac adds is 0.5%～1% of described filtered liquid I weight.
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104844723A (en) * | 2015-06-05 | 2015-08-19 | 云南金九地生物科技有限公司 | Preparation method and application of dendrobium officinale extract |
| CN104892783A (en) * | 2015-05-11 | 2015-09-09 | 南昌大学 | Purification method of dendrobium officinale polysaccharide |
| CN105963314A (en) * | 2016-06-28 | 2016-09-28 | 华侨大学 | Application of dendrobium officinale polysaccharides to preparation of antidepressant medicines and antidepressant healthcare products |
| CN106117385A (en) * | 2016-06-28 | 2016-11-16 | 桐城市雨润生物科技有限公司 | A kind of extraction process of Herba Dendrobii tissue cultured seedling polysaccharide |
| CN106317244A (en) * | 2016-08-29 | 2017-01-11 | 江西九草铁皮石斛科技协同创新有限公司 | Dendrobium officinale polysaccharide as well as preparation method and application thereof |
| CN106674364A (en) * | 2015-11-06 | 2017-05-17 | 广西南宁市邕王医药科技有限公司 | Extracting method for dendrobium polysaccharide |
| CN106692692A (en) * | 2015-08-21 | 2017-05-24 | 中国科学院昆明植物研究所 | Dendrobium officinale extract and preparation method thereof |
| CN107286269A (en) * | 2017-08-11 | 2017-10-24 | 上海海洋大学 | Two kinds of preparation method and applications with immunoregulatory dendrobium candidum leaf polyose |
| CN107474151A (en) * | 2017-08-29 | 2017-12-15 | 霍山县长冲中药材开发有限公司 | A kind of processing method for obtaining dendrobium polysaccharide from the stem of noble dendrobium with alcohol deposition method |
| CN107686526A (en) * | 2017-09-29 | 2018-02-13 | 南京仙草堂生物科技有限公司 | A kind of extraction of dendrobium candidum root dendrobium polysaccharide and purifying process |
| CN107880149A (en) * | 2017-12-29 | 2018-04-06 | 贺州市星辉科技有限公司 | A kind of extraction process of Dendrobium officinale polysaccharide |
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Cited By (17)
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| CN104892783A (en) * | 2015-05-11 | 2015-09-09 | 南昌大学 | Purification method of dendrobium officinale polysaccharide |
| CN104844723A (en) * | 2015-06-05 | 2015-08-19 | 云南金九地生物科技有限公司 | Preparation method and application of dendrobium officinale extract |
| CN106692692A (en) * | 2015-08-21 | 2017-05-24 | 中国科学院昆明植物研究所 | Dendrobium officinale extract and preparation method thereof |
| CN106674364A (en) * | 2015-11-06 | 2017-05-17 | 广西南宁市邕王医药科技有限公司 | Extracting method for dendrobium polysaccharide |
| CN105963314B (en) * | 2016-06-28 | 2021-07-02 | 华侨大学 | Application of dendrobium officinale polysaccharide in preparation of antidepressant and antidepressant health-care product |
| CN105963314A (en) * | 2016-06-28 | 2016-09-28 | 华侨大学 | Application of dendrobium officinale polysaccharides to preparation of antidepressant medicines and antidepressant healthcare products |
| CN106117385A (en) * | 2016-06-28 | 2016-11-16 | 桐城市雨润生物科技有限公司 | A kind of extraction process of Herba Dendrobii tissue cultured seedling polysaccharide |
| CN106317244A (en) * | 2016-08-29 | 2017-01-11 | 江西九草铁皮石斛科技协同创新有限公司 | Dendrobium officinale polysaccharide as well as preparation method and application thereof |
| CN107286269A (en) * | 2017-08-11 | 2017-10-24 | 上海海洋大学 | Two kinds of preparation method and applications with immunoregulatory dendrobium candidum leaf polyose |
| CN107474151A (en) * | 2017-08-29 | 2017-12-15 | 霍山县长冲中药材开发有限公司 | A kind of processing method for obtaining dendrobium polysaccharide from the stem of noble dendrobium with alcohol deposition method |
| CN107686526A (en) * | 2017-09-29 | 2018-02-13 | 南京仙草堂生物科技有限公司 | A kind of extraction of dendrobium candidum root dendrobium polysaccharide and purifying process |
| CN107880149A (en) * | 2017-12-29 | 2018-04-06 | 贺州市星辉科技有限公司 | A kind of extraction process of Dendrobium officinale polysaccharide |
| CN107936131A (en) * | 2017-12-29 | 2018-04-20 | 贺州市星辉科技有限公司 | A kind of technique of acid-base method extraction Dendrobium officinale polysaccharide |
| CN107974475A (en) * | 2017-12-29 | 2018-05-01 | 贺州市星辉科技有限公司 | A kind of technique of enzyme edman degradation Edman from dendrobium candidum leaf extraction polysaccharide |
| CN108794650A (en) * | 2018-08-20 | 2018-11-13 | 云南和瑾科技有限公司 | A kind of dendrobium polysaccharide extracting method |
| CN110478436A (en) * | 2019-08-21 | 2019-11-22 | 四川省峰上生物科技有限公司 | A kind of preparation method of dendrobium candidum extract |
| CN111171173A (en) * | 2020-02-19 | 2020-05-19 | 杭州师范大学 | Method for quickly and efficiently extracting dendrobium officinale polysaccharide |
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