CN106674364A - Extracting method for dendrobium polysaccharide - Google Patents
Extracting method for dendrobium polysaccharide Download PDFInfo
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- CN106674364A CN106674364A CN201510747301.5A CN201510747301A CN106674364A CN 106674364 A CN106674364 A CN 106674364A CN 201510747301 A CN201510747301 A CN 201510747301A CN 106674364 A CN106674364 A CN 106674364A
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Abstract
The invention relates to an extracting method for dendrobium polysaccharide, and belongs to the technical field of traditional Chinese medicine processing. The extracting method comprises the following steps: (a), first, soaking with a 70-80% ethanol aqueous solution, regulating the pH to be 9-11 with sodium hydroxide, filtering, and taking a medicinal residue; (b), pouring boiling water into the medicinal residue so that the ethanol which is soaked in a medicinal material brings polysaccharide quickly; (c) regulating the pH, removing impurities, and removing the impurities with activated carbon to obtain the polysaccharide finally. The extracting method for the dendrobium polysaccharide has the beneficial effects that the extracting rate of the polysaccharide can be remarkably improved; the polysaccharide content in the obtained polysaccharide extract is high.
Description
Technical field
The present invention relates to Chinese medicine processing technique field, and in particular to a kind of extracting method of Dendrobium officinale polysaccharide.
Background technology
Dendrobium candidum for orchid family Dendrobium Sw dendrobium candidum stem, also known as ribbed hedyotis herb.It is medicinal to be recorded in《Legendary god of farming's book on Chinese herbal medicine
Jing》, be classified as top grade, win thin, Qiang Yin with tonifying five zang organs consumptive disease, long term usage thickness stomach and make light of one's life by commiting suicide macrobiosis the effects such as.Afterwards, the successive dynasties
Book on Chinese herbal medicine is on the books.Version in 2010《Pharmacopoeia of People's Republic of China》（One）Its is single-row, carry：It is sweet, it is slightly cold.Return stomach, kidney
Jing.With reinforcing stomach reg fluid, the effect of nourishing Yin and clearing heat.For impairment of yin body fluid deficiency, dry polydipsia, deficiency of stomach-Yin, deficiency of food is retched, empty after being ill
Heat is not moved back.
Jing modern studies find that it is effectively living that dendrobium candidum contains dendrobium polysaccharide, alkaloid, amino acid, fragrant class compound etc.
Property composition, with it is anti-oxidant, antitumor, reduce blood sugar, strengthen immunity, alleviate physical fatigue and the effects such as clearing.In iron sheet
In many active components of the stem of noble dendrobium, dendrobium polysaccharide has very strongly active.Therefore, the number of dendrobium polysaccharide content has become judgement
One of standard of dendrobium candidum quality.Meanwhile, extract polysaccharide component from dendrobium candidum and researched and developed, have become one
Individual study hotspot.
Pertinent literature reports that in stem of noble dendrobium crude drug, the content of polysaccharide is generally 22%.Existing extraction Dendrobium officinale polysaccharide
In method, the shortcomings of recovery rate is not high, and impurity content is high in extraction polysaccharide is there is.Such as number of patent application is
201110365323.7 Chinese invention patent " a kind of isolation and purification method of dendrobium polysaccharide ", using volume ratio 5:1 chloroform：
N-butanol mixed liquor repeats repeatedly de- albumen, while deproteination, is also stripped of polysaccharide, causes polysaccharide extract rate
Reduction.The shortcomings of it also has loaded down with trivial details technique, high cost.
The content of the invention
It is an object of the invention to provide a kind of polysaccharide extract rate is high, and the extraction side of the high Dendrobium officinale polysaccharide of polyoses content
Method.
The technical solution used in the present invention is a kind of extracting method of Dendrobium officinale polysaccharide, is comprised the following steps：
（1）Dendrobium candidum is ground into meal, immersion 0.5-1 hours, hydrogen-oxygen in the 4-8 times of 70-80% ethanol water measured is placed in
Change sodium and adjust pH to 9-11, take out medicinal material, filter, slag of getting it filled；
（2）The boiling water that pH is 9-11 is measured by 4-8 times to be poured into the dregs of a decoction, filtered when temperature is down to 70-80 DEG C, obtain extract；
（3）By the extract of gained salt acid for adjusting pH to neutrality, keeping temperature is concentrated into original volume 1/2nd, filters, and goes out
Precipitation is gone, 1/2nd are further concentrated to, volume weight 0.5-1% activated carbons are added, is kept for 40 DEG C, filtered, concentration is drying to obtain.
Preferably：
（1）Dendrobium candidum is ground into meal, is placed in 75% ethanol water of 6 times of amounts and is soaked 0.5 hour, NaOH is adjusted
Section pH to 10, takes out medicinal material, filters, slag of getting it filled；
（2）The boiling water that pH is 10 is measured by 6 times to be poured into the dregs of a decoction, filtered when temperature is down to 75 DEG C, obtain extract；
（3）By the extract of gained salt acid for adjusting pH to neutrality, keeping temperature is concentrated into original volume 1/2nd, filters, and goes out
Precipitation is gone, 1/2nd are further concentrated to, the activated carbon of volume weight 0.7% is added, is kept for 40 DEG C, filtered, concentration is drying to obtain.
Jing inventor's research finds that lymphatic temperament can wrap up dendrobium candidum in dendrobium candidum traditional extraction in dendrobium candidum
In composition so as to be not easy dissolution, dendrobium candidum epidermis can also form cuticula, and the composition in dendrobium candidum can also do not allowed
Easily dissolution.Therefore, the present invention is using after lower boiling alcohol solution dipping dendrobium candidum, then with high boiling solvent soaking medicine
Material so that solute is simultaneously taken in boiling in the low boiling point solvent short time of immersion medicinal material out of, it is to avoid lymphatic temperament parcel composition, it is not easy to
The phenomenon of dissolution.
It is the alcohol solution dipping medicinal material of 9-11 using pH in the present invention, can effectively improves and extract polysaccharide, and can also removes
The impurity such as deproteinized matter.It has been investigated that, when pH is 10, the recovery rate highest of dendrobium polysaccharide, during less than 9, Polyose extraction can be allowed
Rate is decreased obviously.
Concentrated using substep in the present invention, be segmented the mode of cooling, can effectively remove impurity, and keep polysaccharide yield.
The beneficial effects of the present invention is：
（1）Extracting method of the present invention, can effectively improve the recovery rate of dendrobium polysaccharide, up to 38%.After testing, gained dendrobium polysaccharide
The content of middle dendrobium polysaccharide is more than 97%, and non-polysaccharide component content is few.Compare the side that existing water extract-alcohol precipitation extracts dendrobium polysaccharide
Method, the recovery rate of polysaccharide increases by more than 5%, non-polysaccharide constituent reduction 30% or so in gained Dendrobium officinale polysaccharide.
（2）Extracting method of the present invention, it is scientific and reasonable, it is simple to operate, it is easy to the big production of scale.
Specific embodiment
Embodiment 1
By 1kg dendrobium candidum pulverizing medicinal materials into meal, it is placed in the ethanol waters of 4L 70% and soaks 1 hour, NaOH is adjusted
PH to 11, takes out medicinal material, filters, slag of getting it filled；
The boiling water that 4L pH are 10 is poured into the dregs of a decoction, is filtered when temperature is down to 80 DEG C, obtain extract；
The extract of gained salt acid for adjusting pH to neutrality, keeping temperature are concentrated into 2L, are filtered, precipitation of going out is further concentrated to
1L, adds the activated carbon of volume weight 0.5%, is kept for 40 DEG C, filters, and concentration is drying to obtain.
After testing, the recovery rate of dendrobium polysaccharide is 36%, and wherein polyoses content is 97.5%.The detection foundation of its polyoses content
Version in 2010《Chinese Pharmacopoeia》Polyoses content detection method is carried out under first dendrobium candidum item.
Embodiment 2
By 1kg dendrobium candidum pulverizing medicinal materials into meal, it is placed in the ethanol waters of 8L 80% and soaks 0.5 hour, NaOH is adjusted
Section pH to 9, takes out medicinal material, filters, slag of getting it filled；
The boiling water that 8L pH are 10 is poured into the dregs of a decoction, is filtered when temperature is down to 70 DEG C, obtain extract；
The extract of gained salt acid for adjusting pH to neutrality, keeping temperature are concentrated into 4L, are filtered, precipitation of going out is further concentrated to
2L, adds the activated carbon of volume weight 1%, is kept for 40 DEG C, filters, and concentration is drying to obtain.
After testing, the recovery rate of dendrobium polysaccharide is 36%, and wherein polyoses content is 96%.The detection foundation of its polyoses content
Version in 2010《Chinese Pharmacopoeia》Polyoses content detection method is carried out under first dendrobium candidum item.
Embodiment 3
By 1kg dendrobium candidum pulverizing medicinal materials into meal, it is placed in the ethanol waters of 6L 75% and soaks 0.5 hour, NaOH is adjusted
Section pH to 10, takes out medicinal material, filters, slag of getting it filled；
The boiling water that 6L pH are 10 is poured into the dregs of a decoction, is filtered when temperature is down to 75 DEG C, obtain extract；
The extract of gained salt acid for adjusting pH to neutrality, keeping temperature are concentrated into 3L, are filtered, precipitation of going out is further concentrated to
1.5L, adds the activated carbon of volume weight 0.7%, is kept for 40 DEG C, filters, and concentration is drying to obtain.
After testing, the recovery rate of dendrobium polysaccharide is 38%, and wherein polyoses content is 97.2%.The detection foundation of its polyoses content
Version in 2010《Chinese Pharmacopoeia》Polyoses content detection method is carried out under first dendrobium candidum item.
Claims (2)
1. a kind of extracting method of Dendrobium officinale polysaccharide, it is characterised in that comprise the steps：
（1）Dendrobium candidum is ground into meal, immersion 0.5-1 hours, hydrogen-oxygen in the 4-8 times of 70-80% ethanol water measured is placed in
Change sodium and adjust pH to 9-11, take out medicinal material, filter, slag of getting it filled；
（2）The boiling water that pH is 9-11 is measured by 4-8 times to be poured into the dregs of a decoction, filtered when temperature is down to 70-80 DEG C, obtain extract；
（3）By the extract of gained salt acid for adjusting pH to neutrality, keeping temperature is concentrated into original volume 1/2nd, filters, and goes out
Precipitation is gone, 1/2nd are further concentrated to, volume weight 0.5-1% activated carbons are added, is kept for 40 DEG C, filtered, concentration is drying to obtain.
2. a kind of Dendrobium officinale polysaccharide extracting method according to claim 1, it is characterised in that following steps：
（1）Dendrobium candidum is ground into meal, is placed in 75% ethanol water of 6 times of amounts and is soaked 0.5 hour, NaOH is adjusted
Section pH to 10, takes out medicinal material, filters, slag of getting it filled；
（2）The boiling water that pH is 10 is measured by 6 times to be poured into the dregs of a decoction, filtered when temperature is down to 75 DEG C, obtain extract；
（3）By the extract of gained salt acid for adjusting pH to neutrality, keeping temperature is concentrated into original volume 1/2nd, filters, and goes out
Precipitation is gone, 1/2nd are further concentrated to, the activated carbon of volume weight 0.7% is added, is kept for 40 DEG C, filtered, concentration is drying to obtain.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108623705A (en) * | 2018-06-06 | 2018-10-09 | 完美（中国）有限公司 | A kind of preparation method of dendrobium candidum polysaccharide extractive |
| CN114351498A (en) * | 2021-06-08 | 2022-04-15 | 成都中医药大学 | Process for preparing paper pulp by utilizing edible native angelica sinensis dregs |
| CN114848566A (en) * | 2022-05-16 | 2022-08-05 | 江南大学 | Preparation method and application of dendrobium officinale extract for resisting skin photoaging |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102504041A (en) * | 2011-11-17 | 2012-06-20 | 云南金九地生物科技有限公司 | Separation and purification method for dendrobium polysaccharide |
| CN103772521A (en) * | 2014-01-09 | 2014-05-07 | 云南云尚生物技术有限公司 | Extracting method of dendrobium officinale polysaccharides |
| CN103910810A (en) * | 2014-04-16 | 2014-07-09 | 北京林业大学 | Method for simultaneously preparing dendrobium nobile pigments and dendrobium nobile polysaccharides by using dendrobium nobile raw materials |
| CN104926956A (en) * | 2015-06-26 | 2015-09-23 | 广西健宝石斛有限责任公司 | Method of extracting Dendrobium candidum polysaccharides via ultrasonic optimization |
-
2015
- 2015-11-06 CN CN201510747301.5A patent/CN106674364A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102504041A (en) * | 2011-11-17 | 2012-06-20 | 云南金九地生物科技有限公司 | Separation and purification method for dendrobium polysaccharide |
| CN103772521A (en) * | 2014-01-09 | 2014-05-07 | 云南云尚生物技术有限公司 | Extracting method of dendrobium officinale polysaccharides |
| CN103910810A (en) * | 2014-04-16 | 2014-07-09 | 北京林业大学 | Method for simultaneously preparing dendrobium nobile pigments and dendrobium nobile polysaccharides by using dendrobium nobile raw materials |
| CN104926956A (en) * | 2015-06-26 | 2015-09-23 | 广西健宝石斛有限责任公司 | Method of extracting Dendrobium candidum polysaccharides via ultrasonic optimization |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108623705A (en) * | 2018-06-06 | 2018-10-09 | 完美（中国）有限公司 | A kind of preparation method of dendrobium candidum polysaccharide extractive |
| CN114351498A (en) * | 2021-06-08 | 2022-04-15 | 成都中医药大学 | Process for preparing paper pulp by utilizing edible native angelica sinensis dregs |
| CN114848566A (en) * | 2022-05-16 | 2022-08-05 | 江南大学 | Preparation method and application of dendrobium officinale extract for resisting skin photoaging |
| CN114848566B (en) * | 2022-05-16 | 2023-03-28 | 江南大学 | Preparation method and application of dendrobium officinale extract for resisting skin photoaging |
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Application publication date: 20170517 |