CN104814975A - Anti-fatigue uses of rubus idaeus polysaccharides - Google Patents
Anti-fatigue uses of rubus idaeus polysaccharides Download PDFInfo
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- CN104814975A CN104814975A CN201510164559.2A CN201510164559A CN104814975A CN 104814975 A CN104814975 A CN 104814975A CN 201510164559 A CN201510164559 A CN 201510164559A CN 104814975 A CN104814975 A CN 104814975A
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Abstract
The present invention provides uses of rubus idaeus polysaccharides in preparation of anti-fatigue drugs, health products or food. Study results show that the rubus idaeus polysaccharides have characteristics of good anti-fatigue function, significant social values, and significant market values.
Description
Technical field
The invention belongs to field of health care products, be specifically related to the novelty teabag of Fructus Rubi polysaccharide.
Technical background
When people for a long time, the physical exertion campaign of large intensity time, create a large amount of metabolite in body, as lactic acid, carbon dioxide, serum urea nitrogen etc., this kind of material gathers in vivo, and stimulation human tissue cell and nervous system, will make people produce feeling of fatigue.This fatigue is called physical fatigue.Along with the quickening of modern life rhythm, the aggravation of social competition, the even comprehensive fatigue of mental fatigue, psychological fatigue, pathology fatigue produces one after another, and becomes the health problem perplexing a lot of people.Health is in fatigue state for a long time, can cause the adverse consequences such as hormone in vivo Metabolic disorder, nervous system regulatory function exception, hypoimmunity.
Fructus Rubi (Rubus idaeus L.) has another name called Rubus corchorifolius Linn. f., Fructus Rubi corchorifolii Immaturus, Fructus Rubi etc., belongs to Rosaceae rubus.Fructus Rubi is mainly distributed in temperate zone, the Northern Hemisphere and frigid zone, and its fruit is the little aggregate fruit of many slurries, the sweet micro-acid of taste.Fructus Rubi contains polysaccharide, organic acid, flavone, anthocyanidin, and the various active compositions such as polyphenol, vitamin, mineral element, volatile oil, tannin, have high nutritive value and medical care effect, be described as " gold fruit ".
Yet there are no report Fructus Rubi polysaccharide being used for resisting fatigue.
Summary of the invention
The object of the present invention is to provide the novelty teabag of Fructus Rubi polysaccharide.
Particularly, the invention provides the purposes of Fructus Rubi polysaccharide in the preparation medicine of resisting fatigue, health product or food.
Further, described medicine, health product or food reduce serum urea nitrogen, lactic acid, creatine kinase level, improves blood glucose, the medicine of lactate dehydrogenase levels, health product or food.
Wherein, described medicine, health product or food are peroral dosage form.
Further, described peroral dosage form is selected from tablet, capsule, pill, granule, powder, extractum or oral liquid.
Wherein, in described Fructus Rubi polysaccharide, purity of polysaccharide is more than 50%; Further, purity of polysaccharide is more than 80%; Further, purity of polysaccharide is more than 85%; Preferably, purity of polysaccharide is 85 ~ 95%w/w, if moderate purity of the present invention is 90 ~ 92%.Above-mentioned purity adopts Phenol-sulphate acid method to measure.
Wherein, described Fructus Rubi polysaccharide adopts decoction and alcohol sedimentation technique to prepare.
Further, described decoction and alcohol sedimentation technique concrete operations are as follows:
Get dry Fructus Rubi, after petroleum ether or ether defatting, with concentration 75 ~ 95%v/v ethanol extraction, filter, filtering residue extracting in water, after water intaking extract is concentrated, adds ethanol and reach 75% ~ 85% to alcohol content, cooling, to leave standstill, get solid content and be Fructus Rubi polysaccharide.
Wherein, Fructus Rubi removes seed or does not remove seed before extracting.
Wherein, in precipitate with ethanol process, add ethanol and reach 75% to alcohol content.
Wherein, described Fructus Rubi is the fruit of Rosaceae rubus.
Further, described Fructus Rubi is selected from red raspberry or black raspberry, choice for use red raspberry in the present invention's detailed description of the invention.
The present invention studies discovery, and Fructus Rubi polysaccharide has fine anti-fatigue effect, has significant social value and market value.
Accompanying drawing illustrates:
The impact of accompanying drawing 1 Fructus Rubi Polysaccharides on Mice serum urea nitrogen;
The impact of creatine kinase in accompanying drawing 2 Fructus Rubi Polysaccharides on Mice serum;
The impact of accompanying drawing 3 Fructus Rubi Polysaccharides on Mice blood glucose;
The impact of lactic acid in accompanying drawing 4 Fructus Rubi Polysaccharides on Mice serum;
The impact of lactic acid dehydrogenase in accompanying drawing 5 Fructus Rubi Polysaccharides on Mice serum.
Detailed description of the invention
The Fructus Rubi polysaccharide used in the specific embodiment of the invention adopts conventional Polyose extraction, way of purification prepares, and concrete operations are as follows in the present invention:
Prepared by Fructus Rubi polysaccharide: get dry Fructus Rubi, after seed is removed in pulverizing, add 5 times of petroleum ether (boiling range 60 ~ 90 DEG C) defat, 75 ~ 95%v/v ethanol is added in 60 DEG C of backflows after eliminating petroleum ether, filtering residue after filtering is added 10 times of pure water supersound extraction (power 60W, temperature: 80 DEG C, processing time: 100min), centrifuging and taking supernatant, filtering residue extracts twice again by old terms, merging filtrate, be evaporated to 1/4 of original volume, add 95%v/v ethanol, solution alcohol content is made to reach 75%, in 4 degrees Celsius of standing 24h, collecting by filtration filtering residue, filtering residue dries to obtain Fructus Rubi polysaccharide.
After measured, Fructus Rubi polysaccharide yield prepared by the present invention is 10.4% ~ 11.6%, and obtaining purity of polysaccharide through Phenol-sulphate acid method detection is 90.8% ~ 91.2%.
In various embodiments of the present invention, Fructus Rubi polysaccharide dosage is all in polysaccharide.
Embodiment 1 resisting fatigue is tested
The present invention to utilize in body pharmacodynamic experiment to evaluate the anti-fatigue ability of Fructus Rubi polysaccharide.Specific experiment method is as follows: Kunming kind male and healthy mice (body weight: 25 ± 3g, age: 2 ~ 3 weeks) is divided into 4 groups, is the high, medium and low dosage group of blank group and Fructus Rubi polysaccharide respectively.The dosage of the high, medium and low dosage group of Fructus Rubi polysaccharide is respectively 200mg/kg, 100mg/kg and 50mg/kg, adopts gastric infusion, once a day.Blank group gives equivalent distilled water.Successive administration 14 days.Each group of mice starts to carry out adaptability swimming instruction in administration on the 7th day, and swimming time, by 10 minutes/day, increases progressively 5 minutes by sky.Test last day, mice, after 30 minutes, is placed in the swimming trunk went swimming of depth of water 30cm by administration, and water temperature controls at about 25 DEG C, swims 90 minutes.Have a rest and extract eyeball immediately after 60 minutes and get blood, the blood sample obtained is placed centrifugal (4 DEG C, 2000 turns, 15 minutes) in a moment in room temperature, obtains blood serum sample, frozen for subsequent use in-80 DEG C of refrigerators.
(1) impact of Fructus Rubi Polysaccharides on Mice serum urea nitrogen
The impact of table 1. Fructus Rubi Polysaccharides on Mice serum urea nitrogen
* P < 0.05, * * P < 0.01vs blank group
Serum urea nitrogen is one of important symbol of protein in body metabolism.Motion makes serum urea nitrogen raise, and it is the very sensitive index of evaluation body one of ability to bear when physical load.Body is poorer to load performance, and the increase of serum urea nitrogen is more obvious.As shown in table 1 and Fig. 1, Fructus Rubi polysaccharide significantly can reduce the urea nitrogen content of mice serum, and presents dose-dependence.Wherein, in Fructus Rubi polysaccharide, dosage group has significant difference (* P < 0.05vs blank) compared with blank; Fructus Rubi polysaccharide high dose group has pole significant difference (* * P < 0.01vs blank group) compared with blank.
(2) impact of creatine kinase in Fructus Rubi Polysaccharides on Mice serum
The impact of creatine kinase in table 2. Fructus Rubi Polysaccharides on Mice serum
* P < 0.05, * * P < 0.01vs blank group
Creatine kinase and energy metabolism closely related, it participates in glucolytic control, the energy supply of mitochondrial respiratory machine muscle contraction.Research shows, after a large amount of motion, the creatine kinase activity in serum significantly raises.In serum, the change of creatine kinase can reflect the adaptedness of body to motion, and adaptedness is higher, and serum creatine kinase activity can reduce even recovers normal level.From table 2 Fig. 2, Fructus Rubi polysaccharide obviously can reduce the content of creatine kinase in mice serum, and presents dose-dependence.Wherein, in Fructus Rubi polysaccharide, dosage group has significant difference (* P < 0.05vs blank) compared with blank; Fructus Rubi polysaccharide high dose group has pole significant difference (* * P < 0.01vs blank group) compared with blank.
(3) impact of Fructus Rubi Polysaccharides on Mice blood glucose
The impact of blood glucose in table 3. Fructus Rubi Polysaccharides on Mice serum
* P < 0.01vs blank group
As shown in table 3 and Fig. 3, Fructus Rubi polysaccharide obviously can increase the content of mice serum blood glucose, and presents dose-dependence.Wherein, Fructus Rubi polysaccharide senior middle school dosage group has pole significant difference (* * P < 0.01vs blank group) compared with blank.
(4) impact of lactic acid (LD) content in Fructus Rubi Polysaccharides on Mice serum
The impact of lactic acid in table 4. Fructus Rubi Polysaccharides on Mice serum
* P < 0.01vs blank group
Lactic acid is produced by anaerobic metabolism, and it exists the stability destroying organismic internal environment, affects the normal performance of cardiovascular system, Musculoskeletal function, causes tired generation.In table 4 and Fig. 4, each lactic acid content (* * P < 0.01vs blank group) that all extremely significantly can reduce mice serum of Fructus Rubi polysaccharide, and present dose-dependence.
(5) impact of lactic acid dehydrogenase (LDH) content in Fructus Rubi Polysaccharides on Mice serum
The impact of lactic acid dehydrogenase in table 5. Fructus Rubi Polysaccharides on Mice serum
* P < 0.01vs blank group
Lactic acid in lactic acid dehydrogenase energy decomposer, in timely purged body, lactic acid has very positive effect for the elimination of fatigue.As shown in table 5 and Fig. 5, Fructus Rubi polysaccharide obviously can increase the content of lactic acid dehydrogenase in mice serum, and presents dose-dependence.Wherein, Fructus Rubi polysaccharide senior middle school dosage group has pole significant difference (* * P < 0.01vs blank group) compared with blank.
Above-mentioned experiment all shows, Fructus Rubi polysaccharide has good anti-fatigue effect.
Claims (10)
1. Fructus Rubi polysaccharide is preparing the purposes in the medicine of resisting fatigue, health product or food.
2. purposes according to claim 1, is characterized in that: described medicine, health product or food reduce serum urea nitrogen, lactic acid, creatine kinase level, improves blood glucose, the medicine of lactate dehydrogenase levels, health product or food.
3. purposes according to claim 1 and 2, is characterized in that: described medicine, health product or food are peroral dosage form.
4. the purposes according to claims 1 to 3 any one, is characterized in that: described peroral dosage form is selected from tablet, capsule, pill, granule, powder, extractum or oral liquid.
5. purposes according to claim 1, is characterized in that: in described Fructus Rubi polysaccharide, purity of polysaccharide is more than 50%; Further, purity of polysaccharide is more than 80%; Further, purity of polysaccharide is more than 85%; Preferably, purity of polysaccharide is 85 ~ 95%w/w.
6. the purposes according to Claims 1 to 5 any one, is characterized in that: described Fructus Rubi polysaccharide adopts decoction and alcohol sedimentation technique to prepare.
7. purposes according to claim 6, is characterized in that: described decoction and alcohol sedimentation technique concrete operations are as follows:
Get dry Fructus Rubi, after petroleum ether or ether defatting, with concentration 75 ~ 95%v/v ethanol extraction, filter, filtering residue extracting in water, after water intaking extract is concentrated, adds ethanol and reach 75% ~ 85% to alcohol content, cooling, to leave standstill, get solid content and be Fructus Rubi polysaccharide.
8. purposes according to claim 7, is characterized in that: Fructus Rubi removes seed or do not remove seed before extracting.
9. the purposes according to claim 1 ~ 9 any one, is characterized in that: described Fructus Rubi is the fruit of Rosaceae rubus.
10. the purposes according to claim 1 ~ 9 any one, is characterized in that: described Fructus Rubi is selected from red raspberry or black raspberry.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150353A (en) * | 2019-06-06 | 2019-08-23 | 福建省卡尔顿食品有限公司 | A kind of fermentation bread and preparation method thereof |
| CN111227253A (en) * | 2020-01-20 | 2020-06-05 | 江苏惠田科技开发有限公司 | Blackberry polysaccharide composite breakfast powder and preparation method thereof |
| WO2023000973A1 (en) * | 2021-07-23 | 2023-01-26 | 黄山学院 | Black tea and preparation method therefor |
Citations (2)
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| CN101804127A (en) * | 2010-04-28 | 2010-08-18 | 北京大学第一医院 | Compound traditional Chinese medicine extractive composite for preventing and curing Alzheimer disease |
| CN102319334A (en) * | 2011-08-15 | 2012-01-18 | 深圳康林生物科技有限公司 | Edible Chinese medicine composition |
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2015
- 2015-04-04 CN CN201510164559.2A patent/CN104814975A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101804127A (en) * | 2010-04-28 | 2010-08-18 | 北京大学第一医院 | Compound traditional Chinese medicine extractive composite for preventing and curing Alzheimer disease |
| CN102319334A (en) * | 2011-08-15 | 2012-01-18 | 深圳康林生物科技有限公司 | Edible Chinese medicine composition |
Non-Patent Citations (2)
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| 刘明学等: "覆盆子多糖提取、结构分析及自由基清除作用研究", 《食品科技》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150353A (en) * | 2019-06-06 | 2019-08-23 | 福建省卡尔顿食品有限公司 | A kind of fermentation bread and preparation method thereof |
| CN111227253A (en) * | 2020-01-20 | 2020-06-05 | 江苏惠田科技开发有限公司 | Blackberry polysaccharide composite breakfast powder and preparation method thereof |
| WO2023000973A1 (en) * | 2021-07-23 | 2023-01-26 | 黄山学院 | Black tea and preparation method therefor |
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Application publication date: 20150805 |