CN104926956A - Method of extracting Dendrobium candidum polysaccharides via ultrasonic optimization - Google Patents
Method of extracting Dendrobium candidum polysaccharides via ultrasonic optimization Download PDFInfo
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Abstract
The invention discloses a method of extracting Dendrobium candidum polysaccharides via ultrasonic optimization. The method includes the steps of drying and crushing Dendrobium candidum in vacuum; stirring Dendrobium candidum powder with water according to a solid-solution ratio: 1:30, pouring mixture into a stainless steel barrel, keeping the stainless steel barrel tight, and putting the stainless steel barrel in an ultrasonic extractor for thermal extraction by ultrasonic bath. During extraction, ultrasonic power is 300W to 400W, ultrasonic frequency is 45Hz, extraction temperature is 50 DEG C to 65 DEG C, and temperature-keeping extraction time is 1 hours to 1.5 hours. Compared with the results of conventional water extraction under same conditions, the results of the method show that the average yield of Dendrobium candidum polysaccharides by the ultrasonic optimization extraction is 15.3%, which is 10.2% higher than that of the polysaccharides by the conventional water extraction process under the same conditions.
Description
Technical field
The invention belongs to food processing field, particularly the extracting method of Dendrobium officinale polysaccharide.
Background technology
Herba Dendrobii is the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family Dendrobium, is one of 5 kinds of Dendrobium Sws that the Pharmacopoeia of the People's Republic of China records, in " the Chinese rare plant in imminent danger " of publication in 1989, be just listed in endangered species.Its medicinal part is fresh or dry stem, has the effects such as reinforcing stomach reg fluid, nourishing Yin and clearing heat, hypoglycemic, enhancing is immune and antitumor.
Saccharide compound is extensively distributed in occurring in nature, and is modal composition in plant amedica, and it can be divided into monose, oligose and saccharan three class and their derivative natural gum and lymphatic temperament etc.The monose that wherein polysaccharide refers to more than by 10 molecules dewaters the superpolymer formed.Have many-sided pharmacological action, its effect mainly comprises: strengthen immune, antitumor, anti-bolt of invigorating blood circulation, hypotensive, reducing blood-fat, hypoglycemic, anti-ageing, strengthen marrow hemopoiesis function, radioprotective, provide protection etc. to main organs such as liver kidneys.In nearly 10 years, about the research of polysaccharide is increasing, relevant expert foretells " 21 century will be the epoch of polysaccharide ", the bright prospects of indication polysaccharide researches.
Dendrobium officinale polysaccharide is the principle active component of Traditional Chinese medicine dendrobium candidum, and modern pharmacology research proves: Dendrobium officinale polysaccharide has enhancing body immunizing power, Tumor suppression grows, the power of physiologically active removes the multiple pharmacological effect such as interior free yl.The content of Herba Dendrobii and its polysaccharide has certain relation, and Chang Yiqi height judges the quality of quality of medicinal material.Therefore, the development & application of Dendrobium officinale polysaccharide product and compound Dendrobium officinale polysaccharide product thereof is great for clinical meaning.
Scholar is had to report hot water extraction's technical study of Dendrobium officinale polysaccharide.Ultrasonic technique is the method being commonly used to active ingredients from traditional Chinese medicinal extraction in recent years, and it is by effects such as machinery, calorifics and cavitations, accelerates target component and enters solvent, thus substantially increase extraction efficiency, saved solvent.
In prior art, the report of Dendrobium officinale polysaccharide method is extracted in rare ultrasonic wave optimization.
Summary of the invention
For existing technical problem, the invention provides a kind of ultrasonic wave optimization and extract Dendrobium officinale polysaccharide method, the method can more good utilisation and exploitation Herba Dendrobii resource, effectively improves Dendrobium officinale polysaccharide and extracts yield.
Technical scheme of the present invention is as follows:
Dendrobium officinale polysaccharide method is extracted in a kind of ultrasonic wave optimization, it is characterized in that, comprises the following steps:
(1) by the dry stem of Herba Dendrobii vacuum-drying 2-2.5 hour under 40-60 DEG C of condition;
(2) 70-80 mesh sieve was pulverized;
(3) be that 1:30 adds water and is uniformly mixed rearmounted stainless steel cylinder by dendrobium officinale powder by solid-to-liquid ratio, keep air-tight state, again the container filling feed liquid is joined in ultrasonic extractor and carry out ultrasound bath heating extraction, ultrasonic power is 300W-400W, ultrasonic frequency is 45Hz, Extracting temperature is 50-65 DEG C, insulation extraction time 1h-1.5h;
(4), after extraction terminates, suction filtration, removes the slag dregs of rice, obtains suction filtration mixed solution; By suction filtration mixed solution centrifugation 10-15min under the condition of 5000-6000r/min, gained supernatant liquor is extracting solution;
(5) collect united extraction liquid, concentrate, will add the ethanol of 95% in concentrated solution, limit is stirred, while add, regulates its alcohol concn, staticly carries out alcohol precipitation 8h;
(6) take out precipitation solution, put into whizzer, carry out centrifugal 10min with 15000r/min, obtain Herba Dendrobii Crude polysaccharides precipitation, take out precipitation, and dissolve with aquae destillata;
(7) Crude polysaccharides will extracted, cross macroporous resin, take distilled water as elutriant, elution flow rate is 0.5-1.0ml/min, is further purified Dendrobium officinale polysaccharide;
(8) collect effluent liquid, effluent liquid dialysis tubing is carried out dialysis removing protein, obtains Herba Dendrobii holosaccharide liquid.
Preferably, the ultrasonic frequency in described step (3) is 45-50Hz, and Extracting temperature is 50-65 DEG C, insulation extraction time 1h.
Preferably, the centrifugation 15min under the condition of rotating speed 5500r/min of the suction filtration mixed solution in described step (4).
Relative to prior art, the present invention has following beneficial effect
Contrast with the result of water extraction conventional under equal conditions, the average yield of ultrasonic wave Optimized Extraction Process Dendrobium officinale polysaccharide is 15.3%, the average yield 10.2% of conventional extraction process by water polysaccharide under being better than equal part.
Embodiment
Below in conjunction with embodiment, the present invention will be further described:
Embodiment 1
Dendrobium officinale polysaccharide method is extracted in a kind of ultrasonic wave optimization, it is characterized in that, comprises the following steps:
(1) by the vacuum-drying 2.5 hours under 60 DEG C of conditions of dry for Herba Dendrobii stem;
(2) 70 mesh sieves were pulverized;
(3) be that 1:30 adds water and is uniformly mixed rearmounted stainless steel cylinder by dendrobium officinale powder by solid-to-liquid ratio, keep air-tight state, again the container filling feed liquid is joined in ultrasonic extractor and carry out ultrasound bath heating extraction, ultrasonic power is 300W, ultrasonic frequency is 45Hz, Extracting temperature is 65 DEG C, insulation extraction time 1h;
(4), after extraction terminates, suction filtration, removes the slag dregs of rice, obtains suction filtration mixed solution; By suction filtration mixed solution centrifugation 15min under the condition of 6000r/min, gained supernatant liquor is extracting solution;
(5) collect united extraction liquid, concentrate, will add the ethanol of 95% in concentrated solution, limit is stirred, while add, regulates its alcohol concn, staticly carries out alcohol precipitation 8h;
(6) take out precipitation solution, put into whizzer, carry out centrifugal 10min with 15000r/min, obtain Herba Dendrobii Crude polysaccharides precipitation, take out precipitation, and dissolve with aquae destillata.
(7) Crude polysaccharides will extracted, cross macroporous resin, take distilled water as elutriant, elution flow rate is 0.5-1.0ml/min, is further purified Dendrobium officinale polysaccharide;
(8) collect effluent liquid, effluent liquid dialysis tubing is carried out dialysis removing protein, obtains Herba Dendrobii holosaccharide liquid;
Embodiment 2
Dendrobium officinale polysaccharide method is extracted in a kind of ultrasonic wave optimization, it is characterized in that, comprises the following steps:
(1) by the vacuum-drying 2.5 hours under 50 DEG C of conditions of dry for Herba Dendrobii stem;
(2) 80 mesh sieves were pulverized;
(3) be that 1:30 adds water and is uniformly mixed rearmounted stainless steel cylinder by dendrobium officinale powder by solid-to-liquid ratio, keep air-tight state, again the container filling feed liquid is joined in ultrasonic extractor and carry out ultrasound bath heating extraction, ultrasonic power is 300W, ultrasonic frequency is 45Hz, Extracting temperature is 50-65 DEG C, insulation extraction time 1h-1.5h;
(4), after extraction terminates, suction filtration, removes the slag dregs of rice, obtains suction filtration mixed solution; By suction filtration mixed solution centrifugation 10-15min under the condition of 5000-6000r/min, gained supernatant liquor is extracting solution;
(5) collect united extraction liquid, concentrate, will add the ethanol of 95% in concentrated solution, limit is stirred, while add, regulates its alcohol concn, staticly carries out alcohol precipitation 8h;
(6) take out precipitation solution, put into whizzer, carry out centrifugal 10min with 15000r/min, obtain Herba Dendrobii Crude polysaccharides precipitation, take out precipitation, and dissolve with aquae destillata.
(7) Crude polysaccharides will extracted, cross macroporous resin, take distilled water as elutriant, elution flow rate is 0.5-1.0ml/min, is further purified Dendrobium officinale polysaccharide;
(8) collect effluent liquid, effluent liquid dialysis tubing is carried out dialysis removing protein, obtains Herba Dendrobii holosaccharide liquid;
Embodiment 1 compares with embodiment 2 Dendrobium officinale polysaccharide extraction yield, as shown in table 1 below:
Table 1
| Embodiment 1 | Embodiment 2 | |
| Extraction yield (%) | 15.3％ | 15.8％ |
This specific embodiment is only explanation of the invention; it is not limitation of the present invention; those skilled in the art can make to the present embodiment the amendment not having creative contribution as required after reading this specification sheets, as long as but be all subject to the protection of patent law in right of the present invention.
Claims (3)
1. a Dendrobium officinale polysaccharide method is extracted in ultrasonic wave optimization, it is characterized in that, comprises the following steps:
(1) by the dry stem of Herba Dendrobii vacuum-drying 2-2.5 hour under 40-60 DEG C of condition;
(2) 70-80 mesh sieve was pulverized;
(3) be that 1:30 adds water and is uniformly mixed rearmounted stainless steel cylinder by dendrobium officinale powder by solid-to-liquid ratio, keep air-tight state, again the container filling feed liquid is joined in ultrasonic extractor and carry out ultrasound bath heating extraction, ultrasonic power is 300W-400W, ultrasonic frequency is 45-50Hz, Extracting temperature is 50-65 DEG C, insulation extraction time 1h-1.5h;
(4), after extraction terminates, suction filtration, removes the slag dregs of rice, obtains suction filtration mixed solution; By suction filtration mixed solution centrifugation 10-15min under the condition of rotating speed 5000-6000r/min, gained supernatant liquor is extracting solution;
(5) collect united extraction liquid, concentrate, will add the ethanol of 95% in concentrated solution, limit is stirred, while add, regulates its alcohol concn, staticly carries out alcohol precipitation 8h;
(6) take out precipitation solution, put into whizzer, carry out centrifugal 10min with 15000r/min, obtain Herba Dendrobii Crude polysaccharides precipitation, take out precipitation, and dissolve with distilled water;
(7) Crude polysaccharides will extracted, cross macroporous resin, take distilled water as elutriant, elution flow rate is 0.5-1.0ml/min, is further purified Dendrobium officinale polysaccharide;
(8) collect effluent liquid, effluent liquid dialysis tubing is carried out dialysis removing protein, obtains Herba Dendrobii holosaccharide liquid.
2. a Dendrobium officinale polysaccharide method is extracted in ultrasonic wave optimization, and it is characterized in that, the ultrasonic frequency in described step (3) is 45-50Hz, and Extracting temperature is 50-65 DEG C, insulation extraction time 1h.
3. a Dendrobium officinale polysaccharide method is extracted in ultrasonic wave optimization, and it is characterized in that, the suction filtration mixed solution in described step (4) is centrifugation 15min under the condition of rotating speed 5500r/min.
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105560118A (en) * | 2016-01-21 | 2016-05-11 | 广西大学 | Dendrobium officinale mouth wash |
| CN105906739A (en) * | 2016-07-05 | 2016-08-31 | 镇江劲草堂生物科技有限公司 | Purification method of dendrobium candidum protocorm polysaccharide (DCPP) |
| CN106117385A (en) * | 2016-06-28 | 2016-11-16 | 桐城市雨润生物科技有限公司 | A kind of extraction process of Herba Dendrobii tissue cultured seedling polysaccharide |
| CN106220747A (en) * | 2016-06-23 | 2016-12-14 | 江西九草铁皮石斛科技协同创新有限公司 | Ultrasonic assistant Vacuum cavitation extracts Dendrobium officinale polysaccharide and the preparation technology of instant powder thereof |
| CN106317244A (en) * | 2016-08-29 | 2017-01-11 | 江西九草铁皮石斛科技协同创新有限公司 | Dendrobium officinale polysaccharide as well as preparation method and application thereof |
| CN106362001A (en) * | 2016-09-28 | 2017-02-01 | 江苏春申堂药业有限公司 | Preparation method of dendrobe extract at normal temperature |
| CN106377474A (en) * | 2016-10-09 | 2017-02-08 | 倪飞鹏 | Dendrobium officinale mask and manufacturing method thereof |
| CN106674364A (en) * | 2015-11-06 | 2017-05-17 | 广西南宁市邕王医药科技有限公司 | Extracting method for dendrobium polysaccharide |
| CN107974475A (en) * | 2017-12-29 | 2018-05-01 | 贺州市星辉科技有限公司 | A kind of technique of enzyme edman degradation Edman from dendrobium candidum leaf extraction polysaccharide |
| CN108084291A (en) * | 2018-01-19 | 2018-05-29 | 贵州独秀峰药业有限公司 | The method that polysaccharide is extracted in dendrobium candidum |
| CN108191990A (en) * | 2017-12-29 | 2018-06-22 | 贺州市星辉科技有限公司 | The method of ultrasonic extraction method Dendrobium officinale polysaccharide |
| CN108567599A (en) * | 2018-07-24 | 2018-09-25 | 合肥华盖生物科技有限公司 | A kind of preparation method of high-performance moisture saver mask |
| CN108634314A (en) * | 2018-05-22 | 2018-10-12 | 贵州黔之爱生物科技有限公司 | A kind of production method of dendrobium candidum Rosa roxburghii Tratt lozenge |
| CN108635308A (en) * | 2018-08-07 | 2018-10-12 | 河南城建学院 | A kind of moisturizing radiation-resisting whitening skin care item and preparation method thereof |
| CN109535269A (en) * | 2018-09-26 | 2019-03-29 | 北京明弘科贸有限责任公司 | A kind of preparation process and its application of dendrobium nobile micromolecular polysaccharide extracting solution |
| CN109738427A (en) * | 2019-02-27 | 2019-05-10 | 苏州天成新农生物科技有限公司 | The detection method of polysaccharide in a kind of dendrobium candidum tender shoots |
| CN111690076A (en) * | 2020-06-22 | 2020-09-22 | 浙江亚林生物科技股份有限公司 | Dendrobium pectin extraction process method |
| CN111825779A (en) * | 2020-08-21 | 2020-10-27 | 武汉理工大学 | Extraction method of dendrobium officinale polysaccharide |
| CN114276470A (en) * | 2021-12-28 | 2022-04-05 | 亿利耐雀生物科技有限公司 | Dendrobium polysaccharide and preparation method and application thereof |
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| CN101658639A (en) * | 2009-08-26 | 2010-03-03 | 浙江森宇实业有限公司 | Dendrobium candidum polysaccharide buccal tablet and preparing method thereof |
| CN102715650A (en) * | 2012-06-21 | 2012-10-10 | 浙江中烟工业有限责任公司 | Application of Dendrobium officinale polysaccharide to cigarette |
| CN103340390A (en) * | 2013-06-26 | 2013-10-09 | 广州中医药大学 | Internal heat reducing and inflammation diminishing chili sauce |
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| CN102715650A (en) * | 2012-06-21 | 2012-10-10 | 浙江中烟工业有限责任公司 | Application of Dendrobium officinale polysaccharide to cigarette |
| CN103340390A (en) * | 2013-06-26 | 2013-10-09 | 广州中医药大学 | Internal heat reducing and inflammation diminishing chili sauce |
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| CN106674364A (en) * | 2015-11-06 | 2017-05-17 | 广西南宁市邕王医药科技有限公司 | Extracting method for dendrobium polysaccharide |
| CN105560118A (en) * | 2016-01-21 | 2016-05-11 | 广西大学 | Dendrobium officinale mouth wash |
| CN106220747A (en) * | 2016-06-23 | 2016-12-14 | 江西九草铁皮石斛科技协同创新有限公司 | Ultrasonic assistant Vacuum cavitation extracts Dendrobium officinale polysaccharide and the preparation technology of instant powder thereof |
| CN106220747B (en) * | 2016-06-23 | 2017-06-30 | 江西九草铁皮石斛科技协同创新有限公司 | Ultrasonic assistant Vacuum cavitation extracts the preparation technology of Dendrobium officinale polysaccharide and its instant powder |
| CN106117385A (en) * | 2016-06-28 | 2016-11-16 | 桐城市雨润生物科技有限公司 | A kind of extraction process of Herba Dendrobii tissue cultured seedling polysaccharide |
| CN105906739A (en) * | 2016-07-05 | 2016-08-31 | 镇江劲草堂生物科技有限公司 | Purification method of dendrobium candidum protocorm polysaccharide (DCPP) |
| CN106317244A (en) * | 2016-08-29 | 2017-01-11 | 江西九草铁皮石斛科技协同创新有限公司 | Dendrobium officinale polysaccharide as well as preparation method and application thereof |
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| CN106377474A (en) * | 2016-10-09 | 2017-02-08 | 倪飞鹏 | Dendrobium officinale mask and manufacturing method thereof |
| CN108191990A (en) * | 2017-12-29 | 2018-06-22 | 贺州市星辉科技有限公司 | The method of ultrasonic extraction method Dendrobium officinale polysaccharide |
| CN107974475A (en) * | 2017-12-29 | 2018-05-01 | 贺州市星辉科技有限公司 | A kind of technique of enzyme edman degradation Edman from dendrobium candidum leaf extraction polysaccharide |
| CN108084291A (en) * | 2018-01-19 | 2018-05-29 | 贵州独秀峰药业有限公司 | The method that polysaccharide is extracted in dendrobium candidum |
| CN108634314A (en) * | 2018-05-22 | 2018-10-12 | 贵州黔之爱生物科技有限公司 | A kind of production method of dendrobium candidum Rosa roxburghii Tratt lozenge |
| CN108567599A (en) * | 2018-07-24 | 2018-09-25 | 合肥华盖生物科技有限公司 | A kind of preparation method of high-performance moisture saver mask |
| CN108635308A (en) * | 2018-08-07 | 2018-10-12 | 河南城建学院 | A kind of moisturizing radiation-resisting whitening skin care item and preparation method thereof |
| CN109535269A (en) * | 2018-09-26 | 2019-03-29 | 北京明弘科贸有限责任公司 | A kind of preparation process and its application of dendrobium nobile micromolecular polysaccharide extracting solution |
| CN109738427A (en) * | 2019-02-27 | 2019-05-10 | 苏州天成新农生物科技有限公司 | The detection method of polysaccharide in a kind of dendrobium candidum tender shoots |
| CN111690076A (en) * | 2020-06-22 | 2020-09-22 | 浙江亚林生物科技股份有限公司 | Dendrobium pectin extraction process method |
| CN111825779A (en) * | 2020-08-21 | 2020-10-27 | 武汉理工大学 | Extraction method of dendrobium officinale polysaccharide |
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