CN103340390A - Internal heat reducing and inflammation diminishing chili sauce - Google Patents

Internal heat reducing and inflammation diminishing chili sauce Download PDF

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Publication number
CN103340390A
CN103340390A CN2013102601323A CN201310260132A CN103340390A CN 103340390 A CN103340390 A CN 103340390A CN 2013102601323 A CN2013102601323 A CN 2013102601323A CN 201310260132 A CN201310260132 A CN 201310260132A CN 103340390 A CN103340390 A CN 103340390A
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China
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chilli sauce
internal heat
thick chilli
stem
dendrobium
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CN2013102601323A
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Chinese (zh)
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CN103340390B (en
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魏小勇
胡小红
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广州中医药大学
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Abstract

The invention discloses internal heat reducing and inflammation diminishing chili sauce, which belongs to the technical field of food. The internal heat reducing and inflammation diminishing chili sauce is formed by the following components according to weight percent: 0.05%-1.5% of dendrobium polysaccharides and the balance of conventional chili sauce. According to the invention, the dendrobium polysaccharides are added to the conventional chili sauce, so that the defect that the conventional chili sauce causes internal heat increase and inflammation can be overcome, the chili sauce provided by the invention has the effects of reducing internal heat and diminishing inflammation, a broad market is provided for the chili industry and the traditional Chinese medicine industry, and the chili sauce has good application prospect.

Description

The anti-inflammatory thick chilli sauce reduces internal heat
Technical field
The present invention relates to food technology field, particularly relate to a kind of anti-inflammatory thick chilli sauce that reduces internal heat.
Background technology
Capsicum (Capsicum annuum L.syn.C.frutescens) is Solanaceae Solanaceae Capsicum Capsicum plant, has another name called chilly, hot pepper, excellent green pepper, pimiento, spicy, peppery eggplant etc.Be used as medicine with fruit, root and stem branch.Pepper flavor suffering, hot in nature, the thoughts of returning home, the spleen channel.The chemical composition of having found in the capsicum mainly contains terpene, alkaloids, steroid class, flavonoids, phenols, unrighted acid.
The hot temperature of capsicum can reduce body temperature by sweating, and alleviates myalgia, therefore has stronger antipyretic effect; The active ingredient capsicim of capsicum is a kind of polyphenoils, and it can organize the metabolism of cells involved, thereby stops the canceration process of cell tissue, reduces the incidence of cancer; The fragrance energy saliva stimulating that capsicum is strong and the secretion of gastric juice increase appetite, promote intestines peristalsis, help digest; The capsicim that capsicum is contained can be crossed the metabolism that promotes fat, and the accumulation of control body fat is conducive to the lowering blood-fat and reducing weight diseases prevention.Thick chilli sauce is as the traditional condiments food of China, nutritious, delicious flavour, easy to use, and travelling is edible whenever and wherever possible at home, is subjected to very much consumers in general's favor.In state-owned large market.
But, also contain the alkaloids substance that is called capsaicine in the capsicum, be the main chemical substance that causes capsicum acid, be the main active substances in the capsicum.Capsicim is the pungent vanilla amide alkaloid of a kind of extreme, accounts for 46%~77% of capsaicine.Piquancy component in the capsicum is made up of the congener of multiple structural similarity, and their structure and character and capsicim are very similar, is called capsicim class material.Such material causes thick chilli sauce to be overeated not only can getting angry (the red and swollen wet pain of eyes, abscess of throat, canker sore, the constipation of urine Huang etc.), but also can cause some diseases on the stomach and intestine, be usually expressed as top-heavy, and irritated irritability, hemorrhage, allergy or inflammation can be occurred when serious, the state of an illness will be increased the weight of if be not suitable for eating the people of thick chilli sauce for some after edible.
The clinical manifestation of " getting angry " is that canker sore, words swell and ache, throat discomfort, pain bitter taste halitosis toothache deafness and tinnitus, constipation etc.The traditional Chinese medical science is particular about negative and positive and is in harmonious proportion, and " getting angry " generally all is because yang-energy is contained very much, so regular meeting shows as symptoms such as headache, toothache, abscess of throat, aphthae, eyes redness or irritated insomnia and constipation etc. are red, swollen, hot, pain on symptom.Because the burning hot the easiest consumption of heresy is hindered body body fluid, thus its card except seeing thermal image, common tongue is done few Tianjin again, cool drink, oliguresis, the few symptom of the dried liquid in Tianjin such as be hard and dry.The burning hot heresy train of thought of burning is again compeled the absurd row of blood, so can also cause haematemesis, bleeding from five sense organs or subcutaneous tissue, send out haemorrhages such as spot.If see vexed, thirsty, symptoms such as tongue is red, arteries and veins number also for fire-evil flourishing due to.
Medium molecular substance mainly refers to the oligopeptides material of molecular weight between 300-5000 dalton in the body fluid, it is mainly derived from the catabolite of protein and peptide hormone etc., generally harmless, but its content may present many-sided toxicity if be higher than normal level.And in modern medical theory, the symptom of " getting angry " might be because after the edible maror, the medium molecular substance of body blood plasma acts on the body corresponding site, the activity that suppresses plurality of enzymes, cause sugar, fat, protein and energy metabolism impairment, suppress the formation of immunoglobulin (Ig), suppress biomembranous transport function etc., thereby reduce the resistivity of body, influence that water-electrolyte balance and metabolism etc. finally cause, so " fire-evil, heat poison or the damp and hot heresy " of the similar traditional Chinese medical science of medium molecular substance.Medium molecular substance is to cause a disease it " poison ", so the someone thinks now: disease is poisoned exactly, and curing the disease is exactly detoxification, says with the term of the traditional Chinese medical science, i.e. the meaning of " heresy is gone just from peace ".In molecule total amount level biology increase with some disease condition order of severity closely relatedly, can provide objective indicator for the clinician, the mensuration of medium molecular substance total amount in the blood has the important clinical meaning to the diagnosis of disease, observation of curative effect, prognosis judgement etc.
Solve the enriching yin Chinese medicine of getting angry commonly used at present asparagus fern, the tuber of dwarf lilyturf, radix scrophulariae etc. are arranged; clearing heat-fire Chinese medicine commonly used has rheum officinale, the coptis, golden cypress etc.; removing toxicity for detumescence Chinese medicine commonly used has the capsule of weeping forsythia, honeysuckle, folium isatidis, mulberry leaf, chrysanthemum etc., and purging intense heat Chinese patent drug commonly used has cow-bezoar antitoxic bolus, the Pill of Anemarrhena, Phellodendrom and Rehmannia, cape jasmine golden flower ball etc.Yet, go to take traditional Chinese medicine in order to fall fire, owing to contain multiple components in the Chinese medicine, except playing the effective ingredient of falling fire action, also comprise many unnecessary unknown complex compositions, and these unknown compositions are not only unhelpful to body, may produce harmful side effect on the contrary.
Therefore, because the existence of " getting angry " this phenomenon, want to eat the consumer groups that capsicum and fearness get angry to some and brought puzzlement.And fire falls by the mode that takes traditional Chinese medicine, may cause the side effect that is harmful to again, so, if can develop and a kind ofly can not get angry, even the thick chilli sauce that can reduce internal heat, will have extraordinary market prospects.
Summary of the invention
Based on this, the objective of the invention is to overcome the defective of prior art, a kind of anti-inflammatory thick chilli sauce that reduces internal heat is provided, this thick chilli sauce not only can not cause gets angry and inflammatory reaction, has the effect of the anti-inflammatory of reducing internal heat on the contrary.
For achieving the above object, the present invention takes following technical scheme:
A kind of anti-inflammatory thick chilli sauce that reduces internal heat is characterized in that being made up of following compositions in weight percentage:
Dendrobium polysaccharide 0.05~1.5%
The thick chilli sauce surplus is added into 100%.
Described thick chilli sauce can be the capsicum product of major ingredient with the capsicum for garlic powder and chilli paste, peanut thick chilli sauce, spices thick chilli sauce etc. are any, and namely the thick chilli sauce of routine all can at present.Though in the prior art, existing research to dendrobium polysaccharide, it is effective in anti-oxidant, antitumor, immunological regulation and aspect such as hypoglycemic that but present result of study has only shown dendrobium polysaccharide, is in the effect aspect the anti-inflammatory of reducing internal heat about dendrobium polysaccharide without any report also.The present invention joins dendrobium polysaccharide in the thick chilli sauce, has changed thick chilli sauce in the past and can cause and get angry and the present situation of inflammation, makes the thick chilli sauce of the present invention to have the effect of the anti-inflammatory of reducing internal heat.
Among some embodiment, this thick chilli sauce is made up of following compositions in weight percentage therein:
Dendrobium polysaccharide 0.7~1.5%
The thick chilli sauce surplus is added into 100%.
Among some embodiment, described dendrobium polysaccharide obtains by following steps therein:
1) get stem of noble dendrobium meal, add benzinum by 5~10ml/g, refluxing extraction 1~3h filters and obtains stem of noble dendrobium residue A;
2) adding volumn concentration by 4~7ml/g again is 75~90% ethanol, and refluxing extraction 1~3h filters and obtains stem of noble dendrobium residue B;
3) add water by 45~56ml/g again, at 80~95 ℃, divide 1~3 lixiviate under the condition of 30~60min, filter merging filtrate;
4) again by filtrate: the volume ratio of Sevage reagent is that 1:3~1:0.5 adds Sevage reagent, reaction 25~34min;
5) add volumn concentration be 95~100% ethanol to final solution to contain the ethanol volumn concentration be to leave standstill 1~24h under 75~88%, 4~25 ℃, obtain sediment;
6) sediment that obtains is washed with absolute ethyl alcohol, acetone, ether successively, drying, namely.
In some above-mentioned embodiment, in the step 4), before the adding Sevage reagent, also the merging filtrate that flooding is obtained is concentrated into 1/10th to 1/8th of initial volume, adds activated carbon decolorizing by 0.05~0.20g/100ml, filters, and removes active carbon.
Among some embodiment, described dendrobium polysaccharide obtains by following steps therein:
1) get stem of noble dendrobium meal, add benzinum by 5~10ml/g, refluxing extraction 1-3h filters and obtains stem of noble dendrobium residue C;
2) adding water the back enzyme activity that has activated by 0.6% adding of stem of noble dendrobium meal quality again is the cellulase of 1,400,000 U/g, 45~60 ℃ of enzymolysis 1~3h, and the enzyme that goes out that heats up filters and obtains stem of noble dendrobium residue D;
3) add water by 40~65ml/g again, at 45~60 ℃, divide 2~3 lixiviates under the condition of 1~4h, filter merging filtrate;
4) in merging filtrate, add volumn concentration be 95~100% ethanol to final solution to contain the ethanol volumn concentration be to leave standstill 1~24h under 75~88%, 4~25 ℃, with the drying precipitate that obtains, namely.
In some above-mentioned embodiment, in the step 4), add before the ethanol, also the merging filtrate that flooding is obtained is concentrated into 1/10th to 1/8th of initial volume.
Among some embodiment, described dendrobium polysaccharide obtains by following steps therein:
1) get stem of noble dendrobium meal, add water by 20~50ml/g, 45~60 ℃ of following ultrasonic extraction 1~2.5h remove residue, obtain solution;
2) with the above-mentioned solution concentration that obtains to 1/10th to 1/8th of initial volume, adding volumn concentration is that 95~100% ethanol to final solution ethanol volumn concentration is to leave standstill 1~24h under 75~88%, 4~25 ℃, obtains sediment;
3) get above-mentioned sediment and add water dissolving by 5~10ml/g, press the sediment aqueous solution: the Sevage reagent volume is than adding Sevage reagent for 1:0.5~2:0.5, mixing, leave standstill 15~35min layering, collect water layer, add Sevage reagent as stated above, repeat 3~6 times, with water layer solution concentration drying, namely.
Among some embodiment, described stem of noble dendrobium meal is the dendrobium candidum meal therein.
Compared with prior art, the present invention has following beneficial effect:
The anti-inflammatory thick chilli sauce that reduces internal heat provided by the invention joins in the thick chilli sauce by the active component dendrobium polysaccharide that will have the antiinflammation of reducing internal heat in the stem of noble dendrobium, makes this thick chilli sauce have the effect of the anti-inflammatory of reducing internal heat.Be in particular in the following aspects: 1) this anti-inflammatory thick chilli sauce that reduces internal heat can obviously alleviate the mouse amount of drinking water increase that thick chilli sauce causes, alleviates the symptom of getting angry; 2) this anti-inflammatory thick chilli sauce that reduces internal heat can suppress the mice auricle swelling degree that dimethylbenzene causes and improve vasopermeability; 3) this anti-inflammatory thick chilli sauce that reduces internal heat can suppress that molecule content increases in the mice plasma that thick chilli sauce causes.And above-mentioned effect all is better than adding stem of noble dendrobium water boiling and extraction thing, the bright product of the stem of noble dendrobium are squeezed the juice and the positive drug sanhuang tablets, sanhuang pian.
Because what add is the polysaccharide component that has antiinflammation in the stem of noble dendrobium, add compared with the composition of squeezing the juice with stem of noble dendrobium medicinal extract or bright product, has the better advantage of effect under the identical addition, and unnecessary component content still less in the adding ingredient, have stronger specific aim, the side effect of also as far as possible having avoided other composition may cause human body on the other hand on the one hand.
The invention solves the problem that thick chilli sauce is got angry easily of eating, provide selection for wanting to eat the consumer groups that capsicum and fearness get angry, for capsicum industry and Chinese medicine industry provide vast market, have a good application prospect.
Description of drawings
Fig. 1 is protein content canonical plotting in the experimental example.
The specific embodiment
Describe the present invention in detail below in conjunction with the drawings and specific embodiments.
Embodiment 1
A kind of anti-inflammatory thick chilli sauce that reduces internal heat, composed of the following components:
Thick chilli sauce 39g
Dendrobium polysaccharide 0.5g.
Described thick chilli sauce is garlic powder and chilli paste, is made by following method:
Choose following raw material:
Frying pan is cool again after with the paramount heat of rusting puts into pot to 4 one-tenths heat with all material (except salt, the monosodium glutamate), adds two little spoonfuls of water, and little fire is endured slowly, and with constantly stirring of shovel, steam diminishes behind about 10min, puts into salt, monosodium glutamate.
Described dendrobium polysaccharide is made by following method:
1) gets the about 50g of dendrobium candidum meal, add benzinum (60 ℃~90 ℃) 250ml, refluxing extraction 1h, degreasing is filtered, and discards filtrate, remove little polarity class materials such as oil-soluble impurities in the stem of noble dendrobium plant and interference pigment, volatilize solvent, obtain stem of noble dendrobium residue A;
2) add 80% ethanol 250ml, refluxing extraction 1h filters, and discards filtrate, removes the middle polarity class material in the stem of noble dendrobium plant, volatilizes solvent, obtains stem of noble dendrobium residue B;
3) add distilled water, extract 50min down at 90 ℃, the big polarity class material in the stem of noble dendrobium plant is extracted in lixiviate 2 times (distilled water of twice adding is 2500ml altogether), filters merging filtrate while hot;
4) merging filtrate is evaporated to 250ml, 0.1% activated carbon decolorizing filters, and by filtrate: the volume ratio of Sevage reagent is 1:1 adding Sevage reagent (Sevage reagent is that the volume ratio of chloroform-n-butanol is the solution of 4:1~3:1), except deproteinized, reaction 30min;
5) adding 95% ethanol, to make solution contain alcohol be 80%, and standing over night in refrigerator precipitates the polysaccharide composition;
6) sediment washs successively with absolute ethyl alcohol, acetone, ether, and air dry or oven dry (drying to constant weight for 70 ℃~80 ℃) get dendrobium polysaccharide.
Carry out determination of polysaccharide with phenol sulfuric acid colorimetric method, getting wherein, polysaccharide component is 44%.
Above-mentioned gained thick chilli sauce is mixed with dendrobium polysaccharide, namely get the anti-inflammatory thick chilli sauce that reduces internal heat of present embodiment.
Embodiment 2
A kind of anti-inflammatory thick chilli sauce that reduces internal heat, composed of the following components:
Thick chilli sauce 825g
Dendrobium polysaccharide 12g.
Described thick chilli sauce is the peanut garlic powder and chilli paste, is made by following method:
Choose following raw material:
Frying pan is cool again after with the paramount heat of rusting puts into pot to 4 one-tenths heat with all material (except salt, the monosodium glutamate), adds two little spoonfuls of water, and little fire is endured slowly, and with constantly stirring of shovel, steam diminishes behind about 10min, puts into salt, monosodium glutamate.
Described dendrobium polysaccharide is made by following method:
1) the dry stem of 50g dendrobium candidum is pulverized, crossed 50 mesh sieves, get the dendrobium candidum powder; The dendrobium candidum powder is suspended in the benzinum of 500ml, adds the hot reflux degreasing, filter, discard filtrate, little polarity class materials such as the oil-soluble impurities in the removal stem of noble dendrobium plant and interference pigment are collected filter residue, obtain stem of noble dendrobium residue C;
2) in stem of noble dendrobium residue C, add warm water (20~25 ℃), the enzyme activity that has activated that slowly adds dendrobium candidum quality 0.6% again is the cellulase of 1,400,000 U/g, stir gently, 50 ℃ of constant temperature enzymolysis 1.5h, remove protein ingredient, immediately be warming up to 90 ℃ of enzymes that go out, rapidly cold filtration, collect filter residue, obtain stem of noble dendrobium residue;
3) add the water of 25 times of Dendrobium quality in the filter residue of the enzyme that goes out, lixiviate 2h under 50 ℃ of temperature filters, and adds water lixiviate 2h under 50 ℃ of temperature of 25 times of Dendrobium quality again in the gained filter residue, filtration; Merge the filtrate that adds twice lixiviate, obtain containing the filtrate of polysaccharide composition;
4) merging filtrate is concentrated into 1/10th of original volume, adds absolute ethyl alcohol, make that the volumetric concentration of ethanol reaches 80% in the system, standing over night in 4 ℃ of refrigerators, polysaccharide composition in the stem of noble dendrobium is precipitated, filter, precipitation gets Dendrobium officinale polysaccharide through vacuum drying.
Carry out determination of polysaccharide with phenol sulfuric acid colorimetric method, getting wherein, polysaccharide component is 38%.
Above-mentioned gained thick chilli sauce is mixed with dendrobium polysaccharide, namely get the anti-inflammatory thick chilli sauce that reduces internal heat of present embodiment.
Embodiment 3
A kind of anti-inflammatory thick chilli sauce that reduces internal heat, composed of the following components:
Thick chilli sauce 5250g
Dendrobium polysaccharide 3g.
Described thick chilli sauce is the spices garlic powder and chilli paste, is made by following method:
Choose following raw material:
With chilli chopping, with the warm oil vacuole exhaustion oil content that rises, will drop into anise, cassia bark, Asian puccoon etc. after most of rusting heat more earlier, after waiting to explode perfume (or spice) spices is pulled out, absolute oil is poured in the capsicum, get final product with the Dendrobium officinale polysaccharide mixing.
Described dendrobium polysaccharide is made by following method:
1) the dry stem of 50g dendrobium candidum is pulverized, crossed 50 mesh sieves, get the dendrobium candidum powder; Solid-to-liquid ratio by 1:30 adds water in the dendrobium candidum powder, ultrasonic extraction time 1.5h under 50 ℃; Obtain containing the solution of polysaccharide component, 5000r/min is centrifugal to remove slag, and gets supernatant;
2) with above-mentioned supernatant concentration to ten/one, adding 95% ethanol, to make solution contain alcohol amount be 80%, and the room temperature standing over night precipitates albumen and polysaccharide composition in the solution, and is centrifugal, obtains sediment;
3) get above-mentioned sediment with an amount of dissolved in distilled water, press solution: the Sevage reagent volume is than removing albumen for 1:0.5 adds Sevage reagent, mixing, leave standstill the 30min layering, collect water layer, add Sevage reagent as stated above, repeat 5 times, with water layer solution concentration drying, namely get dendrobium polysaccharide.
Carry out determination of polysaccharide with phenol sulfuric acid colorimetric method, getting wherein, polysaccharide component is 32%.
Above-mentioned gained thick chilli sauce is mixed with dendrobium polysaccharide, namely get the anti-inflammatory thick chilli sauce that reduces internal heat of present embodiment.
The reduce internal heat anti-inflammatory effect experiment of reducing internal heat of anti-inflammatory thick chilli sauce of embodiment 4
1. experiment material
1.1 animal used as test
Kunming mouse, ♂, body weight (20 ± 2) g is provided by Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center, the quality certification number: SCXK (Guangdong) 2008-0020.
1.2 reagent
1.2.1 reagent source: EDTA-2Na(BIOSHARP, Korea S); Sanhuang tablets, sanhuang pian (Huanqiu Pharmaceutical Co., Ltd., Guangdong); Dimethylbenzene (Tianjin Fu Yu Fine Chemical Co., Ltd); Yi Wensilan (Notlas); Glacial acetic acid (Chemical Reagent Co., Ltd., Sinopharm Group); Phosphoric acid (Xilong Chemical Co., Ltd); Ethanol (Guangzhou Chemical Reagent Factory); Coomassie brilliant blue G-250(Sigma); Bovine serum albumin(BSA) (Beijing Suo Laibao Science and Technology Ltd.); DEPC (Guangzhou Wei Jia Science and Technology Ltd.); Perchloric acid (Guangzhou Hui Wang laboratory apparatus Co., Ltd).
1.2.1 reagent preparation
The preparation of protein standard liquid: assay balance takes by weighing the 0.0108g bovine serum albumin(BSA), adds an amount of ddH 2The O dissolving is settled to the 100ml volumetric flask, packing ,-20 ℃ of preservations.
The preparation of Coomassie brilliant blue G-250 dyeing liquor: take by weighing 50mg Coomassie brilliant blue G-250, add the ethanol of 25ml95% and the phosphoric acid of 50ml85%, quantitatively to 500ml, the brown bottle room temperature preservation.
The preparation of calibration curve: get respectively in the test tube of protein standard liquid 0ml, 0.04ml, 0.08ml, 0.12ml, 0.16ml, 0.20ml to 10ml, add ddH 2O to 0.20ml.Add 6ml Coomassie brilliant blue G-250 dyeing liquor respectively in each test tube, shake up, room temperature is placed 15min.Under ultraviolet 590nm wavelength, measure each solution absorbency A.The protein content calibration curve as shown in Figure 1, its calibration curve is Y=0.0017 * X ﹣ 0.0024, the range of linearity is 0~108 μ g/ml, coefficient R 2Be 0.9982, correlation is extremely strong.
Stem of noble dendrobium water boiling and extraction thing: the dendrobium candidum medicinal material of getting clean drying soaks half an hour in 50 times water, and 30~40min is boiled in the back heating, pours out solution A, for head is fried in shallow oil; Add isopyknic water again, 25~30min is boiled in heating, pours out solution B, is two to fry in shallow oil.A is mixed with B, concentrate, drying gets solid, is dendrobium candidum water boiling and extraction thing.
The bright product of the stem of noble dendrobium extract of squeezing the juice: bright dendrobium candidum is cleaned, cut into chunks, mixes with 50 times water, add at a high speed and organize in the pulper, stop behind the 7min, use two-layer filtered through gauze, get filtrate, concentrated, drying gets solid, is the dendrobium candidum aquatic foods product extract of squeezing the juice.
The pre-treatment of model group thick chilli sauce: get the 5g thick chilli sauce and be dissolved in the 5ml pure water, stir, use two-layer filtered through gauze, obtain thick chilli sauce and irritate stomach solution.
The reduce internal heat pre-treatment of anti-inflammatory thick chilli sauce: get the 5g anti-inflammatory thick chilli sauce that reduces internal heat and be dissolved in the 5ml pure water, stir, use two-layer filtered through gauze, the anti-inflammatory thick chilli sauce that obtains reducing internal heat is irritated stomach solution.
The pre-treatment of stem of noble dendrobium water boiling and extraction thing: take by weighing 75mg stem of noble dendrobium water boiling and extraction thing and be dissolved in the 5ml pure water, stir, obtain stem of noble dendrobium water boiling and extraction thing and irritate stomach solution.
The squeeze the juice pre-treatment of extract of the bright product of the stem of noble dendrobium: take by weighing the bright product of the 75mg stem of noble dendrobium extract of squeezing the juice and be dissolved in the 5ml pure water, stir, obtain the stem of noble dendrobium aquatic foods product extract filling stomach solution of squeezing the juice.
1.3 key instrument
GL-21M high speed freezing centrifuge (Shanghai Lu Xiang instrument centrifuge instrument Co., Ltd); Ultraviolet specrophotometer (ULTRASPEC3300Pro); Assay balance (SHIMADZU).
2. method
2.1 the grouping of mouse and raising
Mouse adaptability was fed after 3 days, was divided into 8 groups at random: blank group, model group, 1 group of the anti-inflammatory thick chilli sauce that reduces internal heat, 2 groups of the anti-inflammatory thick chilli sauce that reduce internal heat, 3 groups of the anti-inflammatory thick chilli sauce that reduce internal heat, stem of noble dendrobium water boiling and extraction thing group, the bright product of the stem of noble dendrobium squeeze the juice extract group, positive organize (thick chilli sauce+sanhuang tablets, sanhuang pian).
Wherein, blank group is irritated stomach by every 10g mouse body weight 0.2ml and is given physiological saline, model group gives 0.2ml embodiment 1 by every 10g mouse body weight filling stomach and prepares thick chilli sauce filling stomach solution, the anti-inflammatory thick chilli sauce that reduces internal heat is irritated stomach by every 10g mouse body weight for 1 group and is given the anti-inflammatory thick chilli sauce filling stomach solution that reduces internal heat that 0.2ml embodiment 1 prepares, the anti-inflammatory thick chilli sauce that reduces internal heat is irritated stomach by every 10g mouse body weight for 2 groups and is given the anti-inflammatory thick chilli sauce filling stomach solution that reduces internal heat that 0.2ml embodiment 2 prepares, the anti-inflammatory thick chilli sauce that reduces internal heat is irritated stomach by every 10g mouse body weight for 3 groups and is given the anti-inflammatory thick chilli sauce filling stomach solution that reduces internal heat that 0.2ml embodiment 3 prepares, stem of noble dendrobium water boiling and extraction thing group is irritated stomach by every 10g mouse body weight and is given 0.2ml the above-mentioned stem of noble dendrobium water boiling and extraction thing filling stomach solution for preparing, the bright product of the stem of noble dendrobium extract group of squeezing the juice is irritated stomach by every 10g mouse body weight and is given the bright product of the above-mentioned stem of noble dendrobium for preparing of the 0.2ml extract of squeezing the juice and irritate stomach solution, and positive group is irritated stomach by every 10g mouse body weight and given 0.2ml embodiment 1 and prepare thick chilli sauce filling stomach solution and give 1mg sanhuang tablets, sanhuang pian by every 1kg mouse body weight filling stomach.
2.2 the diet of mouse, drinking-water and sign change situation
Situations such as the drinking-water of observation and record mouse, diet, body weight, defecation, activity.
The anti-inflammatory thick chilli sauce causes the influence experiment of mice ear and capillary permeability in the acute inflammation to getting angry 2.3 reduce internal heat
By the continuous gastric infusion of method in above-mentioned 2.1 5 days, behind the last administration 30min, dimethylbenzene 0.02ml/ was only evenly smeared in the two sides before and after mouse right ear, left ear contrast.After immediately in the molten 0.1ml/10g of mouse tail vein injection 0.5% Yi Wensilan, the glacial acetic acid 0.1ml/10g of lumbar injection 0.6% immediately, get (the EDTA-2Na anti-freezing of eye socket blood behind the 45min, the centrifugal 5min of 5000rpm, get blood plasma and preserve in-70 ℃ of refrigerators, standby) after take off cervical vertebra and put to death, cut two ears along the auricle baseline, lay round auricle (weigh, calculate swelling degree and inhibiting rate) along the same position of left and right sides auricle with the card punch of diameter 7mm.Immediately divide with 6ml physiological saline and wash the abdominal cavity for several times, suction pipe sucking-off cleaning solution in the centrifugal 10min of 3000rpm, is got supernatant, detects protein content with the Coomassie brilliant blue method, measures ultraviolet absorptivity value OD at the 590nm place, record.Swelling degree, swelling degree inhibiting rate, capillary permeability inhibiting rate calculate by following formula, and adopt the SPSS17.0 statistical software to carry out data and handle.Swelling degree=auris dextra weight-left ear weight
Swelling degree inhibiting rate (%)=(model group swelling degree-administration group swelling degree)/blank group swelling degree * 100%
Capillary permeability inhibiting rate (%)=(model group OD value-administration group OD value)/model group OD value * 100%
The anti-inflammatory thick chilli sauce is to the influence of blood plasma medium molecular substance (MMS) 2.4 reduce internal heat
Get the blood plasma 0.1ml that obtains in 2.3 and place the test tube bottom, add 0.5mol/L perchloric acid 0.2ml, vibration immediately evenly.Behind the 2min, after treating fully to react completely, add distilled water 1.1ml.Vibration again suspends precipitation and gets final product, and with the centrifugal 10min of 3000rpm, draws supernatant 1ml in another in vitro, adds distilled water 2ml mixing.At wavelength 254nm place, optical path 1.0cm does blank zeroing with distilled water, measures absorbance.The MMS assay method adopts the biochemical improved Г а б р и з п я нShi of teaching and research room of No.1 Military Medical Univ. (Soviet Union scholar) acid precipitation-ultraviolet absorption method (Wang Zhanren, Xiao Yingqing etc., middle molecular studies progress and assay [J]. Chinese blood purification .2004.3 (2): 102-103).Measurement result is 1 condition name with " condition name (u) " expression with the absorbance 1A of last mensuration.Result's report, sample is unit with decilitre (dl), i.e. " u/dl ", and wherein, 1L=10dl.
3. result
The anti-inflammatory thick chilli sauce is to the influence of mouse amount of drinking water 3.1 reduce internal heat
In the feeding process of mouse, the amount of drinking water situation of record mouse, the result is as shown in table 1.
Table 1 mouse amount of drinking water relatively
Group Amount of drinking water (ml)
Blank group 56.7857±15.80739
Model group 82.5000±14.66419
1 group of anti-inflammatory thick chilli sauce reduces internal heat 60.7143±14.00867
2 groups of anti-inflammatory thick chilli sauce reduce internal heat 61.3451±13.45276
3 groups of anti-inflammatory thick chilli sauce reduce internal heat 61.4582±12.94503
Stem of noble dendrobium water boiling and extraction thing group 72.7120±10.22414
The bright product of the stem of noble dendrobium extract group of squeezing the juice 74.5714±6.13583
Positive group 71.8571±6.68934
By The above results as can be known, the model group mouse amount of drinking water that only gives thick chilli sauce is organized greater than blank, the modeling success is described, and the mouse amount of drinking water of the anti-inflammatory thick chilli sauce 1-3 group of reducing internal heat is all less than model group, what illustrate that embodiment 1-3 prepares reduces internal heat the anti-inflammatory thick chilli sauce after adding dendrobium polysaccharide, has alleviated the phenomenon of getting angry that thick chilli sauce causes.Simultaneously, the mouse amount of drinking water of the anti-inflammatory thick chilli sauce 1-3 that reduces internal heat group also less than stem of noble dendrobium water boiling and extraction thing group, the bright product of the stem of noble dendrobium squeeze the juice extract group and positive group, illustrates that the anti-inflammatory effect of reducing internal heat of dendrobium polysaccharide obviously is better than stem of noble dendrobium water boiling and extraction thing, stem of noble dendrobium aquatic foods product are squeezed the juice and positive drug.
And carry out after data handle with the SPSS17.0 statistical software, obtain a result: the anti-inflammatory thick chilli sauce 1-3 that reduces internal heat group compares with model group, P is all less than 0.01, significant difference is arranged, and, the anti-inflammatory thick chilli sauce 1-3 that reduces internal heat group and stem of noble dendrobium water boiling and extraction thing group and the bright product of the stem of noble dendrobium extract group of squeezing the juice is compared, and the P value also all less than 0.01, has significant difference.The anti-inflammatory thick chilli sauce 1-3 that reduces internal heat group is compared with positive group, and there is significant difference P<0.01.
The anti-inflammatory thick chilli sauce is to the influence of mice ear and vasopermeability 3.2 reduce internal heat
Calculate mice ear and vasopermeability according to the formula in above-mentioned 2.3, the results are shown in following table 2.
Table 2 mice auricle swelling and capillary permeability are relatively
By The above results as can be known, the inhibitory rate of intumesce of the anti-inflammatory thick chilli sauce 1-3 that reduces internal heat group and vasopermeability inhibiting rate all are better than stem of noble dendrobium water boiling and extraction thing group, the bright product of the stem of noble dendrobium squeeze the juice extract group and positive group, even the swelling degree of the anti-inflammatory thick chilli sauce 1-3 group of reducing internal heat is lower than blank the group.Illustrate that the anti-inflammatory thick chilli sauce that reduces internal heat that embodiment 1-3 prepares has inhibiting rate preferably to mice ear in the acute inflammation, reduce the permeability of capillary, the release of inflammatory factor is reduced, the sx of getting angry, thus the anti-inflammatory effect that reduces internal heat is preferably arranged.And carry out obtaining a result after data handle with the SPSS17.0 statistical software: model group and blank group relatively, there is significant difference P<0.05, illustrate that modeling is successful; The anti-inflammatory thick chilli sauce 1-3 that reduces internal heat group compares with model group, and there is significant difference P<0.05; And the anti-inflammatory thick chilli sauce 1-3 that reduces internal heat organizes and stem of noble dendrobium water boiling and extraction thing group, the bright product of the stem of noble dendrobium are squeezed the juice, and extract group and positive group compare, and the P value has significant difference all less than 0.05.
The anti-inflammatory thick chilli sauce is to the influence of molecule content in the mice plasma 3.3 reduce internal heat
Molecule content in calculating according to the method in above-mentioned 2.4, the result is as shown in table 3 below.
Middle molecule content in table 3 mice plasma
By The above results as can be known, molecule content is organized apparently higher than blank in the mice plasma of model group, illustrate that thick chilli sauce causes get angry with blood plasma in the content of medium molecular substance increase relevantly, the modeling success also is described.Molecule content is starkly lower than model group in the mice plasma of the anti-inflammatory thick chilli sauce 1-3 that reduces internal heat group, illustrate that dendrobium polysaccharide in the anti-inflammatory thick chilli sauce 1-3 group of reducing internal heat can reduce to be caused by pungent diet and to get angry, and the effect that medium molecular substance increases, thus reach the fiery effect of dispelling.And molecule content also is lower than stem of noble dendrobium water boiling and extraction thing group, the bright product of the stem of noble dendrobium squeeze the juice extract group and positive group in the mice plasma of the anti-inflammatory thick chilli sauce 1-3 group of reducing internal heat, and illustrate that the dendrobium polysaccharide of the anti-inflammatory thick chilli sauce 1-3 that reduces internal heat in the organizing fiery effect of dispelling is better than stem of noble dendrobium water boiling and extraction thing, stem of noble dendrobium aquatic foods product squeeze the juice extract and positive drug.
And carry out obtaining a result after data handle with the SPSS17.0 statistical software: model group and blank group relatively, the P value has significant difference less than 0.01.The anti-inflammatory thick chilli sauce 1-3 that reduces internal heat group compares with model group, and the P value has significant difference less than 0.01.The anti-inflammatory thick chilli sauce 1-3 that reduces internal heat organizes and stem of noble dendrobium water boiling and extraction thing group, the bright product of the stem of noble dendrobium are squeezed the juice, and extract group and positive group compare, and the P value has significant difference all less than 0.01.
The above embodiment has only expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to claim of the present invention.Should be pointed out that for the person of ordinary skill of the art without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.

Claims (8)

1. anti-inflammatory thick chilli sauce that reduces internal heat is characterized in that being made up of following compositions in weight percentage:
Dendrobium polysaccharide 0.05~1.5%
The thick chilli sauce surplus is added into 100%.
2. the anti-inflammatory thick chilli sauce that reduces internal heat according to claim 1 is characterized in that, is made up of following compositions in weight percentage:
Dendrobium polysaccharide 0.7~1.5%
The thick chilli sauce surplus is added into 100%.
3. the anti-inflammatory thick chilli sauce that reduces internal heat according to claim 1 is characterized in that described dendrobium polysaccharide obtains by following steps:
1) get stem of noble dendrobium meal, add benzinum by 5~10ml/g, refluxing extraction 1~3h filters and obtains stem of noble dendrobium residue A;
2) adding volumn concentration by 4~7ml/g again is 75~90% ethanol, and refluxing extraction 1~3h filters and obtains stem of noble dendrobium residue B;
3) add water by 45~56ml/g again, at 80~95 ℃, divide 1~3 lixiviate under the condition of 30~60min, filter merging filtrate;
4) again by filtrate: the volume ratio of Sevage reagent is that 1:3~1:0.5 adds Sevage reagent, reaction 25~34min;
5) add volumn concentration be 95~100% ethanol to final solution to contain the ethanol volumn concentration be to leave standstill 1~24h under 75~88%, 4~25 ℃, obtain sediment;
6) sediment that obtains is washed with absolute ethyl alcohol, acetone, ether successively, drying, namely.
4. the anti-inflammatory thick chilli sauce that reduces internal heat according to claim 3, it is characterized in that, in the step 4), before adding Sevage reagent, also the merging filtrate that flooding is obtained is concentrated into 1/10th to 1/8th of initial volume, add activated carbon decolorizing by 0.05~0.20g/100ml, filter, remove active carbon.
5. the anti-inflammatory thick chilli sauce that reduces internal heat according to claim 1 is characterized in that described dendrobium polysaccharide obtains by following steps:
1) get stem of noble dendrobium meal, add benzinum by 5~10ml/g, refluxing extraction 1-3h filters and obtains stem of noble dendrobium residue C;
2) adding water the back enzyme activity that has activated by 0.6% adding of stem of noble dendrobium meal quality again is the cellulase of 1,400,000 U/g, 45~60 ℃ of enzymolysis 1~3h, and the enzyme that goes out that heats up filters and obtains stem of noble dendrobium residue D;
3) add water by 40~65ml/g again, at 45~60 ℃, divide 2~3 lixiviates under the condition of 1~4h, filter merging filtrate;
4) in merging filtrate, add volumn concentration be 95~100% ethanol to final solution to contain the ethanol volumn concentration be to leave standstill 1~24h under 75~88%, 4~25 ℃, with the drying precipitate that obtains, namely.
6. the anti-inflammatory thick chilli sauce that reduces internal heat according to claim 5 is characterized in that, in the step 4), adds before the ethanol, and also the merging filtrate that flooding is obtained is concentrated into 1/10th to 1/8th of initial volume.
7. the anti-inflammatory thick chilli sauce that reduces internal heat according to claim 1 is characterized in that described dendrobium polysaccharide obtains by following steps:
1) get stem of noble dendrobium meal, add water by 20~50ml/g, 45~60 ℃ of following ultrasonic extraction 1~2.5h remove residue, obtain solution;
2) with the above-mentioned solution concentration that obtains to 1/10th to 1/8th of initial volume, adding volumn concentration is that 95~100% ethanol to final solution ethanol volumn concentration is to leave standstill 1~24h under 75~88%, 4~25 ℃, obtains sediment;
3) get above-mentioned sediment and add water dissolving by 5~10ml/g, press the sediment aqueous solution: the Sevage reagent volume is than adding Sevage reagent for 1:0.5~2:0.5, mixing, leave standstill 15~35min layering, collect water layer, add Sevage reagent as stated above, repeat 3~6 times, with water layer solution concentration drying, namely.
8. according to each described anti-inflammatory thick chilli sauce that reduces internal heat of claim 3~7, it is characterized in that described stem of noble dendrobium meal is the dendrobium candidum meal.
CN201310260132.3A 2013-06-26 2013-06-26 Internal heat reducing and inflammation diminishing chili sauce CN103340390B (en)

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