CN104382953A - Extraction method of melanin in black fungi - Google Patents
Extraction method of melanin in black fungi Download PDFInfo
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- CN104382953A CN104382953A CN201410700405.6A CN201410700405A CN104382953A CN 104382953 A CN104382953 A CN 104382953A CN 201410700405 A CN201410700405 A CN 201410700405A CN 104382953 A CN104382953 A CN 104382953A
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- auricularia
- melanin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention provides an extraction method of melanin in black fungi. The extraction method comprises the following specific steps: step 1, pretreatment of raw black fungi; step 2, extraction of the crude product of melanin in the black fungi; step 3, precipitation of the crude product of melanin in the black fungi; step 4, drying; step 5, refining of melanin in the black fungi. By virtue of adopting an ultrasonic-assisted extraction process, the extraction method has the advantages of simple extraction process, short extraction time, better extraction effects and the like compared with conventional methods and can conduce to effectively improving the production efficiency.
Description
Technical field
the present invention relates to a kind of extracting method, particularly the melanic extracting method of a kind of Auricularia.
Background technology
auricularia belongs to Eumycota Basidiomycetes Auriculariale, the food that people like, its quality is delicate, clear and melodious smooth, sporophore is except containing except the materials such as rich in protein, vitamin, calcium, phosphorus, copper, also having bioactive polysaccharide containing a large amount of, is a kind of important dietotherapeutic fungus.Auricularia not only has very high nutritive value, and it also has very high medical value.Medical research proves: Auricularia has that QI invigorating is kept fit, nourishing kidney nourishing the stomach, the function such as to invigorate blood circulation, its can anticoagulant, antithrombotic, blood fat reducing, reduce blood viscosity, vessel softening, making blood flow smooth and easy, reduce cardiovascular diseases and occur, is the high edible fungi of a kind of medical value.
melanin (melanin) is the heterogeneous body macromolecular compound by indole or phenolic compound oxidation polymerization, generally in black, brown or brown, be extensively present in animals and plants and microorganism.It is a kind of constitutionally stable complex biological macromole, is insoluble in water, acid and organic solvent.Although melanin is not required in the growth and development process of organism; but there is biological function widely; as reduce organism by ultraviolet radiation, lysozyme enzymolysis, high temperature, oxidation and heavy metal toxicity the injury that brought; improve the viability of organism under some adverse circumstances, and it is that unique organism of protecting known today is from the native endogenous biopolymer of radiation injury.In addition, on respective host, produce melanic ability also relevant to virulence and pathogenicity for certain micro-organisms.
therefore, carry out the extraction purification of Auricularia pigment, be no matter high added value comprehensive utilization and the deep processing development of Auricularia, or to melanic further investigation, all there is very important practical value and theory significance.
Summary of the invention
the object of this invention is to provide the melanic extracting method of a kind of Auricularia.
object of the present invention is achieved through the following technical solutions: the melanic extracting method of a kind of Auricularia, and the concrete steps of this extracting method are:
step 1, Auricularia pretreatment of raw material:
choose without going mouldy, without worm-eaten Auricularia dry products, Auricularia being smashed to pieces, mills with pulverizer, the Auricularia powder after milling is crossed 55 mesh standard sieves, collect black fungus powder and be placed in sealed container, be placed in dry place, for subsequent use;
the melanic crude product of step 2, Auricularia extracts:
use 1.5mol/L sodium hydroxide solution as Extraction solvent, the black fungus powder that step 1 produces is added, lixiviate 40-80 minute under the ultrasonic intensity of 40W-80W, under 10000 turns centrifugal 15 minutes with the ratio of liquid ratio 20-40:1, get supernatant, obtained Auricularia melanin crude product extracting solution;
the precipitation of step 3, Auricularia melanin crude product:
regulating step 2 obtains the pH to 1.5 of Auricularia melanin crude product extracting solution, is placed in 80 DEG C of water-bath 6-12 hour, melanin is precipitated completely, centrifugal 15 minutes of rotating speed 10000, gets precipitation, with deionized water wash 4 times, remove impurity, obtain Auricularia melanin crude product precipitation;
step 4, drying:
step 3 is extracted the Auricularia melanin crude product precipitation obtained, be placed in refrigerator through vacuum lyophilization, preserve under the temperature conditions of-20 DEG C, for subsequent use;
step 5, Auricularia are melanic refining:
get the Auricularia melanin crude product precipitation that step 4 obtains, under 100 DEG C of conditions, 2 hours are hydrolyzed with the HCI solution of 1mol/L, isolated by filtration, use organic solvent respectively again: chloroform, acetic acid ethyl ester, 95% ethanol, process precipitation, the precipitation at every turn obtained all adjusts pH to 2.5 with HCI, and with distilled water wash, finally obtain the Auricularia melanin deposition refined.
beneficial effect of the present invention: the present invention adopts ultrasonic assistant extraction process, has the advantages such as extraction process is simple, extraction time is short, extraction effect is better, can effectively enhance productivity compared with conventional method.
Detailed description of the invention
embodiment 1
1. Auricularia pretreatment of raw material:
auricularia raw material is without going mouldy, without the clean dry products of worm-eaten, first Auricularia is smashed to pieces, fully mill with pulverizer, then the Auricularia powder of milled is crossed 55 mesh standard sieves, collect Auricularia dry powder and be placed in sealed container, saving backup in dry place.
2. the melanic crude product of Auricularia extracts:
with 1.5mol/L sodium hydroxide solution as Extraction solvent, the black fungus powder that step 1 produces is added respectively, lixiviate 40-80 minutes under the ultrasonic intensity of 40W-80W, under 10000 turns centrifugal 15 minutes with the ratio of liquid ratio 20:1-40:1, get supernatant, obtained melanin crude product extracting solution.
3. the melanic precipitation of Auricularia:
regulating step 2 obtains the pH to 1.5 of melanin extracting solution, is placed in 80 DEG C of water-bath 6-12 hour, melanin is precipitated completely, and centrifugal 15 minutes of rotating speed 10000, gets precipitation, with deionized water wash 4 times, removes impurity.
4. dry:
step 3 extracts the crude product melanin deposition obtained, and be placed on-20 DEG C through vacuum lyophilization, Refrigerator store is for subsequent use.
5. Auricularia is melanic refining:
auricularia melanin crude product obtained above is hydrolyzed 2 hours with the HCI solution of 1mol, L under 100 DEG C of conditions, isolated by filtration, organic solvent (chloroform, acetic acid ethyl ester, 95% ethanol) is used repeatedly to process precipitation more respectively, the precipitation at every turn obtained all adjusts pH to 2.5 with HCI, and with distilled water wash, the melanin deposition refined can be obtained.
embodiment 2
1. the pretreatment of Auricularia, with embodiment 1.
2. the melanic crude product of Auricularia extracts:
with 1.5mol/L sodium hydroxide solution as Extraction solvent, the black fungus powder that step 1 produces is added respectively, lixiviate 60 minutes under the ultrasonic intensity of 60W, under 10000 turns centrifugal 15 minutes with the ratio of liquid ratio 30:1, get supernatant, obtained melanin crude product extracting solution.
3. the melanic precipitation of Auricularia:
regulating step 2 obtains the pH to 1.5 of melanin extracting solution, is placed in 80 DEG C of water-baths 10 hours, melanin is precipitated completely, and centrifugal 15 minutes of rotating speed 10000, gets precipitation, with deionized water wash 4 times, removes impurity.
4. dry, with embodiment 1.
5. Auricularia is melanic refining, with embodiment 1.
the above is preferred embodiment of the present invention, is only illustrative for the purpose of the present invention, and nonrestrictive.The all any amendments done within the spirit and principles in the present invention of those skilled in the art, equivalent replacement and improvement etc., all should be included in protection scope of the present invention.
Claims (1)
1. the melanic extracting method of Auricularia, is characterized in that: the concrete steps of this extracting method are:
Step 1, Auricularia pretreatment of raw material:
Choose without going mouldy, without worm-eaten Auricularia dry products, Auricularia being smashed to pieces, mills with pulverizer, the Auricularia powder after milling is crossed 55 mesh standard sieves, collect black fungus powder and be placed in sealed container, be placed in dry place, for subsequent use;
The melanic crude product of step 2, Auricularia extracts:
Use 1.5mol/L sodium hydroxide solution as Extraction solvent, the black fungus powder that step 1 produces is added, lixiviate 40-80 minute under the ultrasonic intensity of 40W-80W, under 10000 turns centrifugal 15 minutes with the ratio of liquid ratio 20-40:1, get supernatant, obtained Auricularia melanin crude product extracting solution;
The precipitation of step 3, Auricularia melanin crude product:
Regulating step 2 obtains the pH to 1.5 of Auricularia melanin crude product extracting solution, is placed in 80 DEG C of water-bath 6-12 hour, melanin is precipitated completely, centrifugal 15 minutes of rotating speed 10000, gets precipitation, with deionized water wash 4 times, remove impurity, obtain Auricularia melanin crude product precipitation;
Step 4, drying:
Step 3 is extracted the Auricularia melanin crude product precipitation obtained, be placed in refrigerator through vacuum lyophilization, preserve under the temperature conditions of-20 DEG C, for subsequent use;
Step 5, Auricularia are melanic refining:
Get the Auricularia melanin crude product precipitation that step 4 obtains, under 100 DEG C of conditions, 2 hours are hydrolyzed with the HCI solution of 1mol/L, isolated by filtration, use organic solvent respectively again: chloroform, acetic acid ethyl ester, 95% ethanol, process precipitation, the precipitation at every turn obtained all adjusts pH to 2.5 with HCI, and with distilled water wash, finally obtain the Auricularia melanin deposition refined.
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| CN201410700405.6A CN104382953A (en) | 2014-11-28 | 2014-11-28 | Extraction method of melanin in black fungi |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108261427A (en) * | 2018-01-16 | 2018-07-10 | 黑龙江大学 | Low iron hematinic, black fungus melanin activity ingredient F1 and application thereof |
| CN108542923A (en) * | 2018-05-22 | 2018-09-18 | 福建农林大学 | A kind of application from the melanin extracted in black fungus in liver-protecting product |
| CN108841200A (en) * | 2018-05-22 | 2018-11-20 | 福建农林大学 | A kind of preparation method of water solubility agaric melanin |
| CN109439017A (en) * | 2018-11-29 | 2019-03-08 | 福建农林大学 | A kind of method that enzyme-ultrasonic synergistic extracts Uricularia polytricha melanin |
| CN109549959A (en) * | 2018-11-29 | 2019-04-02 | 福建农林大学 | A method of black fungus melanin is extracted using cellulase-ultrasonic synergistic |
| CN112592605A (en) * | 2020-12-18 | 2021-04-02 | 中国计量大学 | Black fungus melanin and preparation and application thereof |
| CN114698769A (en) * | 2022-03-15 | 2022-07-05 | 大连工业大学 | Method for immobilizing black pigment of agaric and application of agaric in preparation of jelly |
| CN114736563A (en) * | 2022-05-30 | 2022-07-12 | 山东中医药大学 | Edible antioxidant protective film on bezoar surface and preparation method thereof |
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| CN102229754A (en) * | 2011-05-12 | 2011-11-02 | 华中农业大学 | Method for extracting melanin from waste tremella cultivating material |
| CN102766658A (en) * | 2012-07-30 | 2012-11-07 | 南京农业大学 | Production process of Jew's ear melanin by fermentation and products of Jew's ear melanin |
| US8815539B1 (en) * | 2013-06-06 | 2014-08-26 | River Road Research, Inc. | Methods for producing melanin and inorganic fertilizer from fermentation leachates |
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2014
- 2014-11-28 CN CN201410700405.6A patent/CN104382953A/en active Pending
Patent Citations (3)
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| CN102229754A (en) * | 2011-05-12 | 2011-11-02 | 华中农业大学 | Method for extracting melanin from waste tremella cultivating material |
| CN102766658A (en) * | 2012-07-30 | 2012-11-07 | 南京农业大学 | Production process of Jew's ear melanin by fermentation and products of Jew's ear melanin |
| US8815539B1 (en) * | 2013-06-06 | 2014-08-26 | River Road Research, Inc. | Methods for producing melanin and inorganic fertilizer from fermentation leachates |
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| 李琦等: "黑木耳黑色素鉴定及提取工艺优化", 《食品科学》 * |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108261427A (en) * | 2018-01-16 | 2018-07-10 | 黑龙江大学 | Low iron hematinic, black fungus melanin activity ingredient F1 and application thereof |
| CN108261427B (en) * | 2018-01-16 | 2023-09-15 | 黑龙江大学 | Low-iron blood replenishing agent, black fungus melanin active ingredient F1 and application thereof |
| CN108542923A (en) * | 2018-05-22 | 2018-09-18 | 福建农林大学 | A kind of application from the melanin extracted in black fungus in liver-protecting product |
| CN108841200A (en) * | 2018-05-22 | 2018-11-20 | 福建农林大学 | A kind of preparation method of water solubility agaric melanin |
| CN108841200B (en) * | 2018-05-22 | 2019-11-15 | 福建农林大学 | A kind of preparation method of water solubility agaric melanin |
| CN109439017A (en) * | 2018-11-29 | 2019-03-08 | 福建农林大学 | A kind of method that enzyme-ultrasonic synergistic extracts Uricularia polytricha melanin |
| CN109549959A (en) * | 2018-11-29 | 2019-04-02 | 福建农林大学 | A method of black fungus melanin is extracted using cellulase-ultrasonic synergistic |
| CN109549959B (en) * | 2018-11-29 | 2021-07-23 | 福建农林大学 | Method for extracting black fungus melanin by using cellulase-ultrasonic wave synergism |
| CN112592605A (en) * | 2020-12-18 | 2021-04-02 | 中国计量大学 | Black fungus melanin and preparation and application thereof |
| CN114698769A (en) * | 2022-03-15 | 2022-07-05 | 大连工业大学 | Method for immobilizing black pigment of agaric and application of agaric in preparation of jelly |
| CN114736563A (en) * | 2022-05-30 | 2022-07-12 | 山东中医药大学 | Edible antioxidant protective film on bezoar surface and preparation method thereof |
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