CN105532350B - A kind of preparation method of hainan holly leaf ginkgo biloba extract compound tea - Google Patents
A kind of preparation method of hainan holly leaf ginkgo biloba extract compound tea Download PDFInfo
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- CN105532350B CN105532350B CN201510973607.2A CN201510973607A CN105532350B CN 105532350 B CN105532350 B CN 105532350B CN 201510973607 A CN201510973607 A CN 201510973607A CN 105532350 B CN105532350 B CN 105532350B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/08—Oxidation; Fermentation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/14—Tea preparations, e.g. using additives
Abstract
The invention discloses a kind of preparation methods of hainan holly leaf ginkgo biloba extract compound tea, belong to tea technical field.It includes pretreatment before picking, the preparation of Shanlu Tea extract for dropping, the preparation of ginkgo biloba extract, hybrid packed, obtains the hainan holly leaf ginkgo biloba extract compound tea of active component content high (especially flavones content height).The present invention is fully combined hainan holly leaf effect and ginkgo effect, overcomes the defect of the single effect of tea, is a kind of compound tea preparation method of extract with good development prospect.
Description
Technical field
The present invention relates to a kind of preparation methods of hainan holly leaf ginkgo biloba extract compound tea, belong to tea technical field.
Background technology
Hainan holly leaf, also known as hainan holly leaf, cold in nature, bitter calm down liver through entering liver, spleen, lung, kidney, have it is clearing heat and detoxicating,
The effect of swelling and pain relieving, blood circulation and channel invigorating.It can be used for caused by lowering blood pressure and blood fat, norcholesterol, coronary heart disease, cerebrovascular accident
Hemiplegia and anemopyretic cold, cough with lung heat, edema of throat, tonsillitis, dysentery.This is because in hainan holly leaf containing flavonoids,
Triterpene acids, triterpene saponin, the chemical compositions such as Coumarins, chlorogenic acid also contain certain trace element.Wherein, yellow
Letones are the main actives of hainan holly leaf, with reducing blood lipid, inhibition hyperplasia of prostate, anti-nonalcoholic fatty liver etc.
Effect.As 101214269 B of Chinese patent CN disclose one kind《The method of Extraction and enrichment total flavones of ilex hainanensis》, this method is
It is achieved：The alcohol reflux extraction for being 40-95% with weight concentration, upper HPD-400 macroreticular resins, are used after extracting solution concentration
Sweat ethanol gradient elution, collects the part of a concentration of 20-50% ethanol elutions, dry after recycling design, you can.This method
The selective deposition activity ingredient in extraction process removes other compositions and impurity, obtains the general flavone more than 50% and contains
Amount.
Ginkgo also known as gingko, Gong Sunshu, duck bottom sediment, duck's foot tree are ancient Chinese medicinal plants for high megaphanerophyte of falling leaves
One of.In particular, ginkgo leaf is wide one of the Chinese medicine of current Clinical practice, this is because ginkgo leaf has invigorating heart, promoting blood circulation
It relieves pain, the healthcare function relievingd asthma of astringing the lung, is played an important role in the treatment of cardiovascular and cerebrovascular disease.In order to widen answering for ginkgo
With range, people have carried out various researchs to it, as 102613339 B of Chinese patent CN disclose one kind《A kind of ginkgo
Garlic Pu'er cooked tea composite particle beverage》, which is prepared by the raw material of following weight percents：Ginkgo biloba p.e 1
~5%, black garlic juice 1~4%, Pu-Er ripe tea extract 1~5%, gingko benevolence juice 80~85%, surplus is auxiliary material, main
Functional instant is realized, the absorptivity and health-care efficacy of beverage are improved；101449805 B of CN disclose one kind《One kind from
The method that ginkgo biloba extract is extracted in ginkgo leaf》, this method is mainly by the mass ratio ginkgo leaf raw material that is 1: 0.1~2 and solid
Phase reagent is dissolved through ball milling, and filtering takes supernatant, and pH value, separation, purifying is adjusted to obtain ginkgo biloba p.e.This method tool simultaneously
Have that environmental-friendly, product purity is high, dissolvent residual is few, easy to operate, with short production cycle, the advantages such as production cost is low.
Although the research of hainan holly leaf and ginkgo has been carried out the several years, through retrieving the prior art, still not yet send out so far
The research report of the existing compound tea for closing their extracts composition.In view of there are multiple efficacies therefore to grind for hainan holly leaf and ginkgo
Studying carefully a kind of preparation method of hainan holly leaf ginkgo biloba extract compound tea is very important.
Invention content
The technical problem to be solved in the present invention is overcome the deficiencies in the prior art, and the present invention provides a kind of hainan holly leaf silver
The preparation method of apricot extract compound tea.
The technical solution that the present invention is taken in order to solve the above problem is：
It is 50% that hainan holly leaf ginkgo biloba extract compound tea of the present invention, which is by mass percent,:50% hainan holly leaf carries
Object and ginkgo biloba extract is taken to form.
The preparation method of hainan holly leaf ginkgo biloba extract compound tea of the present invention includes following technique：Pre- place before picking
Reason, the preparation of Shanlu Tea extract for dropping, the preparation of ginkgo biloba extract and hybrid packed.
(1) pretreatment before picking：
S1) growth the age be 3 years hainan holly leaf trees root 20cm locate, dig length 10~15 ㎝, wide 10~15 ㎝, deeply 10~
Hainan holly leaf raw material is prepared fertilizer and is applied in hole by the hole of 15 ㎝, every plant of 100~150 grams of dosage, covers table soil, every
It carries out within 15~20 days once, is carried out continuously 2 times；
S2) hainan holly leaf raw material is extracted to obtain extracting solution dilute 100~150 times, with high-pressure sprayer to the tree of hainan holly leaf
Root, trunk, leaf spray hainan holly leaf extracting solution, carry out once, being carried out continuously 2 times, adopting after 15 days after sprinkling every 15~20 days
It plucks；
(2) preparation of Shanlu Tea extract for dropping：
Sa) first by 40~50 DEG C of the hainan holly leaf tender leaf of picking, tender shoots hot water wash cleans, and then with 90~100 DEG C
10% sodium chloride solution impregnates 10~15 minutes, then pulls out after draining away the water, is put into 60~80 DEG C of drying box, and baking 45~
60 minutes, it is finally cooled to room temperature, is crushed into powder, and crosses 40~60 mesh sieve, obtains hainan holly leaf particle；
Sb) will be saccharomycete by weight ratio：Lactic acid bacteria=(15~20)：(25~35) the mixed bacteria stoste access formed
Step (Sa) in hainan holly leaf particle, and ferment 150~180 hours at 30~40 DEG C, wherein mixed bacteria stoste accounts for hainan holly leaf
The weight ratio of particle is 1~3%；
Sc) by the hainan holly leaf progress drying and processing after fermentation process, elder generation dries 20~30 points in 100~110 DEG C of baking oven
Clock, then it is 60~80 DEG C to adjust drying temperature, is dried 60~70 minutes；
Sd) by step (Sc) in hainan holly leaf be 40~60kHz, extract twice at 40~50 DEG C in supersonic frequency, for the first time
Add the distilled water of 8 times of weight, extract 10~15 minutes, second of the distilled water for adding 3 times of weight is extracted 4~8 minutes, filtered, closes
And filtrate, filtrate is placed on 4000~5000 turns of centrifuge and is centrifuged 10~15 minutes, is filtered again, is concentrated, it is dry, it obtains
Shanlu Tea extract for dropping powder；
(3) preparation of ginkgo biloba extract：
The preparation of ginkgo biloba extract is to adopt to prepare with the aforedescribed process, i.e., repeatedly step (1), (2) prepare ginkgo biloba extract
Powder；
(4) hybrid packed：
Shanlu Tea extract for dropping powder and ginkgo biloba extract powder are distinguished into spray-dried, crushing and cross 400~500 mesh
Sieve；Then it is 50% by mass percentage:50% is sufficiently mixed uniformly, you can obtains hainan holly leaf ginkgo biloba extract compound tea.
The hainan holly leaf raw material is prepared fertilizer and is made of each component of following weight：Hainan holly leaf waste material
40~50 parts, 4~6 parts of hainan holly leaf extracting solution, 7~9 parts of rice straw, 3~5 parts of powdered rice hulls, 10~15 parts of chicken manure, chicken feather 0.3~0.7
Part, 2~4 parts of cow dung.
Preferably,
The hainan holly leaf raw material is prepared fertilizer and is made of each component of following weight：Hainan holly leaf waste material
45 parts, 5 parts of hainan holly leaf extracting solution, 8 parts of rice straw, 4 parts of powdered rice hulls, 13 parts of chicken manure, 0.5 part of chicken feather, 3 parts of cow dung.
The hainan holly leaf waste material include leftover bits and pieces in hainan holly leaf process after non-compliant raw material, processing or
Hainan holly leaf extracts a kind of and/or more than one mixture in residue.
The hainan holly leaf raw material extracts to obtain extracting solution, refers to weighing standard compliant hainan holly leaf, adds water to cook three times,
The distilled water for adding 8 times of weight for the first time, decocts 80~90 minutes in 100 DEG C, second of the distilled water for adding 6 times of weight, in 105
It is decocted 40~50 minutes in DEG C, the distilled water of third time plus 3 times of weight decocts 20~30 minutes in 110 DEG C, filters, and merges
Filtrate, obtained hainan holly leaf extracting solution.
Using preparation method identical with hainan holly leaf, to prepare, fertilizer is prepared in ginkgo raw material and ginkgo raw material extracts
To extracting solution.
Compared with prior art, the invention has the advantages that：
1, contain a variety of active ingredients in hainan holly leaf, ginkgo, make compound tea set just because of containing these active constituents
Having has the effects that heat-clearing, blood pressure lowering, hypoglycemic.The present invention to hainan holly leaf (ginkgo) before picking by applying hainan holly leaf (ginkgo)
Fertilizer is prepared in raw material and hainan holly leaf (ginkgo) raw material extracts to obtain extracting solution, and includes abundant in fertilizer, extracting solution
Hainan holly leaf (ginkgo) active constituent, active constituent not only slowly penetrate into blade from hainan holly leaf (ginkgo) leaf surface layer, Er Qiecong
Root is slowly absorbed into blade, increases the content of the active constituent of hainan holly leaf (ginkgo) leaf.
2, the present invention takes full advantage of the leftover bits and pieces after non-compliant raw material, processing in hainan holly leaf (ginkgo) process
Material or hainan holly leaf (ginkgo) extract residue, and obtain extracting solution by the way that fertilizer is prepared to hainan holly leaf (ginkgo) raw material, extracts,
It is applied to tree root, trunk and the leaf of the preceding hainan holly leaf (ginkgo) of picking, it is made to turn waste into wealth, reduces production cost, is increased economical
Benefit.
3, it is impregnated using 10% sodium chloride solution, forms concentration difference with hainan holly leaf (ginkgo) blade, increase cell membrane and wear
Permeability promotes oxidation enzyme r e lease, improves compound tea quality and effect；Also use the production of fermented by mixed bacterium technology multiple simultaneously
Tea is closed, the defect of single culture is not only overcome, but also the mouthfeel and effect of compound tea can be improved, to make answering for compound tea
It is more extensive with range, improve the market competitiveness.
Specific implementation mode
Embodiment 1
A kind of preparation method of hainan holly leaf ginkgo biloba extract compound tea, includes the following steps：
(1) pretreatment before picking：
S1) growth the age be 3 years hainan holly leaf trees root 20cm locate, dig length 10~15 ㎝, wide 10~15 ㎝, deeply 10~
Hainan holly leaf raw material is prepared fertilizer and is applied in hole by the hole of 15 ㎝, every plant of 125 grams of dosage, cover table soil, every 18 days into
Row is primary, is carried out continuously 2 times；
The hainan holly leaf raw material is prepared fertilizer and is made of each component of following weight：Hainan holly leaf waste material
45 parts, 5 parts of hainan holly leaf extracting solution, 8 parts of rice straw, 4 parts of powdered rice hulls, 13 parts of chicken manure, 0.5 part of chicken feather, 3 parts of cow dung；
The hainan holly leaf waste material is non-compliant raw material in hainan holly leaf process；
The hainan holly leaf raw material extracts to obtain extracting solution, refers to weighing standard compliant hainan holly leaf, adds water to cook three times,
The distilled water for adding 8 times of weight for the first time, decocts 85 minutes in 100 DEG C, second of the distilled water for adding 6 times of weight, in 105 DEG C
It decocts 45 minutes, the distilled water of third time plus 3 times of weight decocts 25 minutes in 110 DEG C, filters, merging filtrate, obtained mountain
Green tea extractive liquor；
S2) extract hainan holly leaf raw material to obtain 125 times of extracting solution dilution, tree root from high-pressure sprayer to hainan holly leaf, tree with
Dry, leaf sprays hainan holly leaf extracting solution, carries out once, being carried out continuously 2 times, picking after 15 days after sprinkling every 18 days；
(2) preparation of Shanlu Tea extract for dropping：
Sa) first by 45 DEG C of the hainan holly leaf tender leaf of picking, tender shoots hot water wash cleans, and then with 95 DEG C of 10% sodium chloride
Solution impregnates 12 minutes, then pulls out after draining away the water, is put into 70 DEG C of drying box, dries 50 minutes, is finally cooled to room temperature,
It is crushed into powder, and crosses 60 mesh sieve, obtain hainan holly leaf particle；
Sb) will be saccharomycete by weight ratio：Lactic acid bacteria=18：The mixed bacteria stoste access step (S of 30 compositionsa) in
Hainan holly leaf particle, and ferment 170 hours at 35 DEG C, wherein the weight ratio that mixed bacteria stoste accounts for hainan holly leaf particle is 2%；
Sc) dry the hainan holly leaf progress drying and processing after fermentation process 25 minutes, then adjust first in 105 DEG C of baking oven
Drying temperature is 70 DEG C, is dried 65 minutes；
Sd) by step (Sc) in hainan holly leaf be 50kHz, extract twice at 45 DEG C in supersonic frequency, for the first time plus 8 times of weights
The distilled water of amount extracts 12 minutes, and second of the distilled water for adding 3 times of weight extracts 6 minutes, filtering, merging filtrate, by filtrate
It is placed on 4500 turns of centrifuge and centrifuges 12 minutes, filter again, concentrate, it is dry, obtain Shanlu Tea extract for dropping powder；
(3) preparation of ginkgo biloba extract：
The preparation of ginkgo biloba extract is to adopt to prepare with the aforedescribed process, i.e., repeatedly step (1), (2) prepare ginkgo biloba extract
Powder；
(4) hybrid packed：
Shanlu Tea extract for dropping powder and ginkgo biloba extract powder are distinguished into spray-dried, crushing and cross 500 mesh sieve；Then
It is 50% by mass percentage:50% is sufficiently mixed uniformly, you can obtains hainan holly leaf ginkgo biloba extract compound tea.
The measurement of flavones content is detected using HPLC methods in hainan holly leaf ginkgo biloba extract compound tea, and after testing, flavones contains
Amount is 39.52%.
Embodiment 2
A kind of preparation method of hainan holly leaf ginkgo biloba extract compound tea, includes the following steps：
(1) pretreatment before picking：
S1) growth the age be 3 years hainan holly leaf trees root 20cm locate, dig length 10~15 ㎝, wide 10~15 ㎝, deeply 10~
Hainan holly leaf raw material is prepared fertilizer and is applied in hole by the hole of 15 ㎝, every plant of 100 grams of dosage, cover table soil, every 15 days into
Row is primary, is carried out continuously 2 times；
The hainan holly leaf raw material is prepared fertilizer and is made of each component of following weight：Hainan holly leaf waste material
40 parts, 4 parts of hainan holly leaf extracting solution, 7 parts of rice straw, 3 parts of powdered rice hulls, 10 parts of chicken manure, 0.3 part of chicken feather, 2 parts of cow dung；
The hainan holly leaf waste material is the leftover bits and pieces after processing；
The hainan holly leaf raw material extracts to obtain extracting solution, refers to weighing standard compliant hainan holly leaf, adds water to cook three times,
The distilled water for adding 8 times of weight for the first time, decocts 80 minutes in 100 DEG C, second of the distilled water for adding 6 times of weight, in 105 DEG C
It decocts 40 minutes, the distilled water of third time plus 3 times of weight decocts 20 minutes in 110 DEG C, filters, merging filtrate, obtained mountain
Green tea extractive liquor；
S2) extract hainan holly leaf raw material to obtain 100 times of extracting solution dilution, tree root from high-pressure sprayer to hainan holly leaf, tree with
Dry, leaf sprays hainan holly leaf extracting solution, carries out once, being carried out continuously 2 times, picking after 15 days after sprinkling every 15 days；
(2) preparation of Shanlu Tea extract for dropping：
Sa) first by 40 DEG C of the hainan holly leaf tender leaf of picking, tender shoots hot water wash cleans, and then with 90 DEG C of 10% sodium chloride
Solution impregnates 10 minutes, then pulls out after draining away the water, is put into 60 DEG C of drying box, dries 45 minutes, is finally cooled to room temperature,
It is crushed into powder, and crosses 40 mesh sieve, obtain hainan holly leaf particle；
Sb) will be saccharomycete by weight ratio：Lactic acid bacteria=15：The mixed bacteria stoste access step (S of 25 compositionsa) in
Hainan holly leaf particle, and ferment 150 hours at 30 DEG C, wherein the weight ratio that mixed bacteria stoste accounts for hainan holly leaf particle is 1%；
Sc) dry the hainan holly leaf progress drying and processing after fermentation process 20 minutes, then adjust first in 100 DEG C of baking oven
Drying temperature is 60 DEG C, is dried 60 minutes；
Sd) by step (Sc) in hainan holly leaf be 40kHz, extract twice at 40 DEG C in supersonic frequency, for the first time plus 8 times of weights
The distilled water of amount extracts 10 minutes, and second of the distilled water for adding 3 times of weight extracts 4 minutes, filtering, merging filtrate, by filtrate
It is placed on 4000 turns of centrifuge and centrifuges 10 minutes, filter again, concentrate, it is dry, obtain Shanlu Tea extract for dropping powder；
(3) preparation of ginkgo biloba extract：
The preparation of ginkgo biloba extract is to adopt to prepare with the aforedescribed process, i.e., repeatedly step (1), (2) prepare ginkgo biloba extract
Powder；
(4) hybrid packed：
Shanlu Tea extract for dropping powder and ginkgo biloba extract powder are distinguished into spray-dried, crushing and cross 400 mesh sieve；Then
It is 50% by mass percentage:50% is sufficiently mixed uniformly, you can obtains hainan holly leaf ginkgo biloba extract compound tea.
The measurement of flavones content is detected using HPLC methods in hainan holly leaf ginkgo biloba extract compound tea, and after testing, flavones contains
Amount is 37.46%.
Embodiment 3
A kind of preparation method of hainan holly leaf ginkgo biloba extract compound tea, includes the following steps：
(1) pretreatment before picking：
S1) growth the age be 3 years hainan holly leaf trees root 20cm locate, dig length 10~15 ㎝, wide 10~15 ㎝, deeply 10~
Hainan holly leaf raw material is prepared fertilizer and is applied in hole by the hole of 15 ㎝, every plant of 150 grams of dosage, cover table soil, every 20 days into
Row is primary, is carried out continuously 2 times；
The hainan holly leaf raw material is prepared fertilizer and is made of each component of following weight：Hainan holly leaf waste material
50 parts, 6 parts of hainan holly leaf extracting solution, 9 parts of rice straw, 5 parts of powdered rice hulls, 15 parts of chicken manure, 0.7 part of chicken feather, 4 parts of cow dung；
The hainan holly leaf waste material is that hainan holly leaf extracts residue；
The hainan holly leaf raw material extracts to obtain extracting solution, refers to weighing standard compliant hainan holly leaf, adds water to cook three times,
The distilled water for adding 8 times of weight for the first time, decocts 90 minutes in 100 DEG C, second of the distilled water for adding 6 times of weight, in 105 DEG C
It decocts 50 minutes, the distilled water of third time plus 3 times of weight decocts 30 minutes in 110 DEG C, filters, merging filtrate, obtained mountain
Green tea extractive liquor；
S2) extract hainan holly leaf raw material to obtain 150 times of extracting solution dilution, tree root from high-pressure sprayer to hainan holly leaf, tree with
Dry, leaf sprays hainan holly leaf extracting solution, carries out once, being carried out continuously 2 times, picking after 15 days after sprinkling every 20 days；
(2) preparation of Shanlu Tea extract for dropping：
Sa) first by 50 DEG C of the hainan holly leaf tender leaf of picking, tender shoots hot water wash cleans, and then with 100 DEG C of 10% chlorination
Sodium solution impregnates 15 minutes, then pulls out after draining away the water, is put into 80 DEG C of drying box, dries 60 minutes, is finally cooled to room
Temperature is crushed into powder, and crosses 60 mesh sieve, obtains hainan holly leaf particle；
Sb) will be saccharomycete by weight ratio：Lactic acid bacteria=20：The mixed bacteria stoste access step (S of 35 compositionsa) in
Hainan holly leaf particle, and ferment 180 hours at 40 DEG C, wherein the weight ratio that mixed bacteria stoste accounts for hainan holly leaf particle is 3%；
Sc) dry the hainan holly leaf progress drying and processing after fermentation process 30 minutes, then adjust first in 110 DEG C of baking oven
Drying temperature is 80 DEG C, is dried 70 minutes；
Sd) by step (Sc) in hainan holly leaf be 60kHz, extract twice at 50 DEG C in supersonic frequency, for the first time plus 8 times of weights
The distilled water of amount extracts 15 minutes, and second of the distilled water for adding 3 times of weight extracts 8 minutes, filtering, merging filtrate, by filtrate
It is placed on 5000 turns of centrifuge and centrifuges 15 minutes, filter again, concentrate, it is dry, obtain Shanlu Tea extract for dropping powder；
(3) preparation of ginkgo biloba extract：
The preparation of ginkgo biloba extract is to adopt to prepare with the aforedescribed process, i.e., repeatedly step (1), (2) prepare ginkgo biloba extract
Powder；
(4) hybrid packed：
Shanlu Tea extract for dropping powder and ginkgo biloba extract powder are distinguished into spray-dried, crushing and cross 500 mesh sieve；Then
It is 50% by mass percentage:50% is sufficiently mixed uniformly, you can obtains hainan holly leaf ginkgo biloba extract compound tea.
The measurement of flavones content is detected using HPLC methods in hainan holly leaf ginkgo biloba extract compound tea, and after testing, flavones contains
Amount is 35.21%.
The applicant carries out experimental study to the compound tea of the embodiment of the present invention 1~3, and specific test method is as follows：
1, be cold cough by 15~20g of weight and clinical symptoms, generate heat the mouse 120 of (38~39 DEG C of body temperature), with
Machine is divided into A groups, B groups and C groups, every group 40；
2, A groups take the compound tea of the preparation of embodiment 1, B groups take the compound tea of the preparation of embodiment 2 and C groups take implementation
Compound tea prepared by example 3；
3, instructions of taking：Three times a day, each 10mg takes 7 days；
4, temperature taking is carried out to the mouse taken 7 days, and records data.
It finds after tested, mouse temperature is less than 38 DEG C：A groups have 27；B groups have 23；C groups have 25.If with Mice Body
Temperature is standard less than 38 DEG C, then A groups 67.5%, B of effective percentage groups 57.5%, C of effective percentage groups effective percentage 62.5%.It can be seen that
Has effects that heat-clearing using compound tea of the present invention.
In addition, the applicant also studies the compound tea of the embodiment of the present invention 1~3 as follows：
1, by the mouse 120 that 15~20g of weight and clinical symptoms are hypertension (130~140mmHg of systolic pressure), at random
It is divided into A groups, B groups and C groups, every group 40；
2, A groups take the compound tea of the preparation of embodiment 1, B groups take the compound tea of the preparation of embodiment 2 and C groups take implementation
Compound tea prepared by example 3；
3, instructions of taking：Three times a day, each 10mg takes 30 days；
4, blood pressure test is carried out to the mouse taken 30 days, and records data.
It finds after tested, mouse blood pressure is less than 125mmHg：A groups have 26；B groups have 23；C groups have 25.If with mouse
Blood pressure is standard less than 125mmHg, then A groups 65.0%, B of effective percentage groups 57.5%, C of effective percentage groups effective percentage 62.5%.Thus
As it can be seen that there is blood pressure reduction effect using compound tea of the present invention.
Finally, the applicant also studies 1 compound tea of the embodiment of the present invention as follows：
1, by the mouse 120 that 15~20g of weight and clinical symptoms are hyperglycemia (8.0~9.0mmol/L of fasting blood-glucose),
It is randomly divided into A groups, B groups and C groups, every group 40；
2, A groups take embodiment 1 preparation compound tea, B groups take hainan holly leaf and C groups take ginkgo leaf tea；
3, instructions of taking：Three times a day, each 10mg takes 30 days；
4, blood sugar test is carried out to the mouse taken 30 days, and records data.
It finds after tested, mouse blood sugar is in 3.5~7.0mmol/L：A groups have 28；B groups have 23；C groups have 25.If
It is less than in 3.5~7.0mmol/L as standard using mouse blood sugar, then A groups 70.0%, B of effective percentage groups effective percentage 57.5%, C groups have
Efficiency 62.5%.It can be seen that there is effect of lowering blood sugar using compound tea of the present invention.
Certainly, it is that the preferred embodiments of the disclosure is described in detail above, the present invention is not limited with this
Practical range, equivalence changes made by all principles under this invention, construction and structure should all be covered by the protection of the present invention
In range.
Claims (3)
1. a kind of preparation method of hainan holly leaf ginkgo biloba extract compound tea, it is characterised in that：Include the following steps：
(1) pretreatment before picking：
S1) locate in the root 20cm that the growth age is 3 years hainan holly leaf trees, dig 10~15cm of length, wide 10~15cm, deeply 10~15cm
Hole, is prepared fertilizer by hainan holly leaf raw material and is applied in hole, every plant of 100~150 grams of dosage, table soil is covered, every 15~20
It once, be carried out continuously 2 times；
S2) extract hainan holly leaf raw material to obtain 100~150 times of extracting solution dilution, tree root from high-pressure sprayer to hainan holly leaf, tree with
Dry, leaf sprays hainan holly leaf extracting solution, carries out once, being carried out continuously 2 times, picking after 15 days after sprinkling every 15~20 days；
(2) preparation of Shanlu Tea extract for dropping：
Sa) first by 40~50 DEG C of the hainan holly leaf tender leaf of picking, tender shoots hot water wash cleans, and then with the 10% of 90~100 DEG C
Sodium chloride solution impregnates 10~15 minutes, then pulls out after draining away the water, is put into 60~80 DEG C of drying box, dries 45~60 points
Clock is finally cooled to room temperature, is crushed into powder, and crosses 40~60 mesh sieve, obtains hainan holly leaf particle；
Sb) will be saccharomycete by weight ratio：Lactic acid bacteria=(15~20)：(25~35) the mixed bacteria stoste formed accesses step
(Sa) the hainan holly leaf particle in, and ferment 150~180 hours at 30~40 DEG C, wherein mixed bacteria stoste accounts for hainan holly leaf particle
Weight ratio be 1~3%；
Sc) dry the hainan holly leaf progress drying and processing after fermentation process 20~30 minutes first in 100~110 DEG C of baking oven, then
It is 60~80 DEG C to adjust drying temperature, is dried 60~70 minutes；
Sd) by step (Sc) in hainan holly leaf be 40~60kHz, extract twice at 40~50 DEG C in supersonic frequency, for the first time plus 8
The distilled water of times weight extracts 10~15 minutes, and the distilled water of second plus 3 times of weight is extracted 4~8 minutes, filtered, merges
Filtrate is placed on 4000~5000 turns of centrifuge and centrifuges 10~15 minutes, filters again by filtrate, concentrates, dry, obtains mountain
Green-tea extract powder；
(3) preparation of ginkgo biloba extract：
S1) locate in the root 20cm that the growth age is 3 years ginkgo, dig long 10~15cm, wide 10~15cm, deeply 10~15cm
Hole, is prepared fertilizer by ginkgo raw material and is applied in hole, every plant of 100~150 grams of dosage, table soil is covered, every 15~20 days
Once, be carried out continuously 2 times；
S2) ginkgo raw material is extracted to obtain extracting solution dilute 100~150 times, with tree root from high-pressure sprayer to ginkgo, trunk, tree
Foliar spray spills ginkgo extract, carries out once, being carried out continuously 2 times, picking after 15 days after sprinkling every 15~20 days；
S3) first by 40~50 DEG C of the ginkgo tender leaf of picking, tender shoots hot water wash cleans, and then with 90~100 DEG C of 10% chlorination
Sodium solution impregnates 10~15 minutes, then pulls out after draining away the water, is put into 60~80 DEG C of drying box, dries 45~60 minutes,
It is finally cooled to room temperature, is crushed into powder, and crosses 40~60 mesh sieve, obtains ginkgo particle；
S4) will be saccharomycete by weight ratio：Lactic acid bacteria=(15~20)：(25~35) the mixed bacteria stoste formed accesses step
(S3) in ginkgo particle, and ferment 150~180 hours at 30~40 DEG C, wherein mixed bacteria stoste accounts for the weight of ginkgo particle
Amount is than being 1~3%；
S5) dry the ginkgo progress drying and processing after fermentation process 20~30 minutes, then adjust first in 100~110 DEG C of baking oven
It is 60~80 DEG C to save drying temperature, is dried 60~70 minutes；
S6) by step (S5) in ginkgo be 40~60kHz, extract twice at 40~50 DEG C in supersonic frequency, for the first time plus 8 times
The distilled water of weight extracts 10~15 minutes, second of the distilled water for adding 3 times of weight, extracts 4~8 minutes, and filtering merges filter
Filtrate is placed on 4000~5000 turns of centrifuge and centrifuges 10~15 minutes, filters again by liquid, concentrates, dry, obtains ginkgo
Extract powder；
(4) hybrid packed：
Shanlu Tea extract for dropping powder and ginkgo biloba extract powder are distinguished into spray-dried, crushing and cross 400~500 mesh sieve；So
It is 50% by mass percentage afterwards：50% is sufficiently mixed uniformly, you can obtains hainan holly leaf ginkgo biloba extract compound tea.
2. the preparation method of hainan holly leaf ginkgo biloba extract compound tea according to claim 1, it is characterised in that：The mountain
Green tea materials are prepared fertilizer and are made of each component of following weight：40~50 parts of hainan holly leaf waste material, hainan holly leaf
4~6 parts of extracting solution, 7~9 parts of rice straw, 3~5 parts of powdered rice hulls, 10~15 parts of chicken manure, 0.3~0.7 part of chicken feather, 2~4 parts of cow dung.
3. the preparation method of hainan holly leaf ginkgo biloba extract compound tea according to claim 1, it is characterised in that：The mountain
Green tea materials extract to obtain extracting solution, are to weigh hainan holly leaf, add water to cook three times, add the distilled water of 8 times of weight for the first time, in
It is decocted 80~90 minutes in 100 DEG C, second of the distilled water for adding 6 times of weight decocts 40~50 minutes, third time in 105 DEG C
The distilled water for adding 3 times of weight decocts 20~30 minutes in 110 DEG C, filtering, what merging filtrate obtained.
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