CN105994791A - Preparation method and application of mulberry leaf instant tea - Google Patents
Preparation method and application of mulberry leaf instant tea Download PDFInfo
- Publication number
- CN105994791A CN105994791A CN201610348102.1A CN201610348102A CN105994791A CN 105994791 A CN105994791 A CN 105994791A CN 201610348102 A CN201610348102 A CN 201610348102A CN 105994791 A CN105994791 A CN 105994791A
- Authority
- CN
- China
- Prior art keywords
- folium mori
- preparation
- instant tea
- time
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/18—Extraction of water soluble tea constituents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/166—Addition of, or treatment with, enzymes or microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method and application of mulberry leaf instant tea. The preparation method comprises the following steps of: adopting fresh mulberry leaves as preparation raw materials; and adopting a drying process, a crushing process, a primary extracting process, a primary filter process, a microwave-assisted compound enzyme treatment process, a secondary extracting process, a secondary filter process, a process of combining and extracting extract liquor obtained twice, a drying process and a pelletizing process; an microwave-assisted compound enzyme extracting technology is used for sufficiently breaking and dehydrating cellulose and pectin substances, so that release and dissolution of functional ingredients in mulberry leaves are increased, and therefore, the yield of gamma-aminobutyric acid with a health value and content of functional components such as 1-deoxynojirimycin are improved. The prepared mulberry leaf instant tea is strong in instant solubility, has remarkable healthcare effect of reducing blood sugar, reducing fat and reducing pressure for three-high crowd; the processing method is strong in maneuverability and is beneficial to industrial development of national mulberry leaf instant tea.
Description
Technical field
The present invention relates to the manufacture field of health tea, particularly relate to the preparation method of a kind of Folium Mori instant tea and answer
With.
Background technology
The most a large amount of crowds have the physiological phenomenon of blood fat, blood glucose, slight Hypertension, highly desirable with natural
Plant is the conditioning of the health care beverage of raw material.Folium Mori are that health ministry confirms " integration of edible and medicinal herbs " plant,
Listed in " one of mankind's 21 century ten big health food by international food health organization.Folium Mori tea is being permitted in recent years
Many countries and regions are fast-developing and come into vogue, and the process technology of external Folium Mori tea has tended to ripe, and China
Being in development period, the Folium Mori tea making China produce is lacked competitiveness in the international market.
Folium Mori tea is presently mainly and presses green tea technique, completes, and after pulverizing, packed bubble is drunk, but Folium Mori in major part
Effective ingredient is all wrapped in cellulose and the pectin of plant cell wall, is difficult to destroy cell with hot water extraction
Wall, extraction ratio is low.How to improve its preparation technology to protect to strengthen it with the release and dissolution increasing both effectiveness composition
Strong effect is the direction of current Folium Mori tea subject study.
Summary of the invention
Goal of the invention: in order to the effective component extraction rate overcoming Folium Mori tea at present present in prior art is low, nothing
Method plays the problem of maximum health-care effect, and the present invention proposes one and is sufficiently destroyed and hydrocellulose and pectin thing
Matter, increases release and the dissolution of both effectiveness composition, and the Folium Mori that instant capacity is strong and processing method is workable are instant
The preparation method and application of tea.
Technical scheme: in order to solve above-mentioned technical problem, the technical solution adopted in the present invention is: a kind of Folium Mori
The preparation method of instant tea, preparing raw material is new fresh mulberry leaf, specifically includes following steps: stoving process, pulverizing
Technique, for the first time extraction process, for the first time filtering technique, microwave-assisted are combined enzyme treatment process, second time carries
Taking technique, for the second time filtering technique, the merging of twice extracting solution and concentration technology, drying process and granulation work
Skill；Wherein twice extraction process all uses the purified water of Quality of Mulberry Leaves 10～20 times to extract at a temperature of 95 DEG C
2h, described microwave-assisted compound enzyme treatment process condition is microwave power 300～1200W, temperature 35～55 DEG C,
PH 5.0～6.0, the time 4～10h.
Being more highly preferred to, described stoving process is: by fresh Folium Mori raw material roguing, 80 DEG C of air blast are dried to former
The 20～28% of weight；Described disintegrating process is: the Folium Mori after drying carry out pulverizing 20～40 mesh sieves；Powder
Leaching after broken, accelerates leaching speed；Described first time extraction process is: by the mass ratio of Folium Mori with water be
The ratio of 1:10～1:20 is put in leaching can, heats the most while stirring, heats 95 DEG C, dimension
Hold 2h hour；Described first time filtering technique is: by the extracting solution after stirring leaching in the leaching can sealed
It is cooled to room temperature, first carries out initial filter with 60～120 mesh stainless steel filtering nets, then be 0.5 μm with pore size filter
Ceramic membrane carry out fine straining, operating condition is that temperature is less than 65 DEG C, entrance pressure 2.5～3.4bar, go out pressure 0.2～
1.3bar；Described microwave-assisted is combined enzyme treatment process: the Folium Mori filtering residue after filtering adds Folium Mori dry weight
In the compound enzyme aqueous solution of 4～6 times, control extraction pot microwave power 300～1200W, temperature 35～55 DEG C,
PH 5.0～6.0, the time 4～10h；Described second time extraction process is: to science and engineering at microwave-assisted compound enzyme
Solution benefit purified water after skill process, to 10～20 times of Quality of Mulberry Leaves, heats the most while stirring,
Heat 95 DEG C, maintain 2h；Described second time filtering technique is with filtering technique for the first time；Described twice extracting solution
Merging technique and concentration technology be: merge twice filtrate and concentrate, thickening temperature 80～90 DEG C, vacuum
Degree is 0.10～0.02MPa, and being concentrated into solid content is 18～30%；Described drying process is: carried out by concentrated solution
Being spray-dried, spray drying tower inlet temperature 130 DEG C～150 DEG C, temperature of outgoing air is more than 75 DEG C；Described granulating process
For: do binding agent after concentrated solution after filtering with leaching, pelletize with dried powder bonding, concentrated solution and dried powder
Mass ratio be 1:0.4～1:0.8, regulation compressed air pressure for spraying 0.2～0.3MPa, injection: 0.1～
0.2MPa, peristaltic pump rotating speed 70～90r/min, < 80 DEG C, Granulation time is 50～90min to control temperature.
Further, described microwave-assisted is combined pH regulator in enzyme treatment process and uses citric acid.
Be more highly preferred to, in described compound enzyme aqueous solution compound enzymic preparation addition be Folium Mori dry weight 0.2%～
0.9%.
Being more highly preferred to, the proportioning of described compound enzymic preparation is calculated by mass is: cellulase 50%～80%,
Pectase 15%～30%, the xylanase of surplus.
Being more highly preferred to, the average enzyme of described compound enzymic preparation is lived as 10000-50000 unit.Enzyme unit alive is abided by
From 1961, international zymetology meeting regulation, referred under specified conditions (25 DEG C, other is optimal condition),
The enzyme amount of 1 micromole substrate, or 1 micromolar relevant group in conversion of substrate can be converted in 1 minute
Enzyme amount.The present invention utilizes microwave-assisted enzymatic isolation method, decomposition, softening fibre element, destroys cell wall, promotes merit
Effect property composition discharges and dissolution, and enzyme addition is important factor, and enzyme amount is inadequate, can extend and affect at processing
The reason time, quality of finished is caused to decline, along with increasing addition, the effective ingredient output increased of extraction, but increase
After adding to a certain extent, though effective ingredient increased, but it is to increase limited, also improves cost, shadow simultaneously
Ring productivity effect.
Being more highly preferred to, described microwave-assisted compound enzyme treatment process condition is temperature 45 C, pH 5.5, time
Between 6h.
The invention allows for the application of a kind of Folium Mori instant tea, be applied particularly to three-hypers crowd and normal population fall
Blood fat, blood sugar lowering, the health food of blood pressure lowering, have significant health-care effect.
Beneficial effect: the preparation method and application of a kind of Folium Mori instant tea that the present invention provides, uses new fresh mulberry leaf
For preparing raw material, use stoving process, disintegrating process, for the first time extraction process, for the first time filtering technique, micro-
The merging of ripple assisted recombination enzyme treatment process, for the second time extraction process, for the second time filtering technique, twice extracting solution
And concentration technology, drying process and granulating process are prepared from；The use of microwave-assisted multiplex-enzyme extraction technology,
It is sufficiently destroyed and hydrocellulose and pectin substance, increases release and the dissolution of both effectiveness composition in Folium Mori, thus
Improve its both effectiveness component content such as the gamma aminobutyric acid yield with health value and 1-DNJ,
The Folium Mori instant tea instant capacity that the present invention prepares is strong, shows the health-care effect of three-hypers crowd's blood sugar lowering, blood fat reducing, blood pressure lowering
Write, and processing method is workable, contributes to the industry development of China's Folium Mori instant tea beverage.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail:
Embodiment 1:
A kind of preparation method of Folium Mori instant tea:
(1) stoving process: by fresh Folium Mori raw material roguing, 80 DEG C of air blast are dried to the 20～28% of original weight；
(2) disintegrating process: the Folium Mori after drying carry out pulverizing and sieving 20～40 mesh；
(3) extraction process for the first time: Folium Mori are put into the extraction pot of band microwave and temperature control, by people's Folium Mori 12
The purified water of times weight, heats while stirring, heats 95 DEG C, maintains 2h；
(4) filter for the first time: after having extracted, the tea after stirring leaching is starched in the leaching can sealed cold
But to room temperature, first initial filter is carried out with 80 mesh stainless steel filtering nets, then with the ceramic membrane that pore size filter is 0.5 μm
Carrying out fine straining, operating condition is that temperature is less than 65 DEG C, and entrance pressure 3.0bar goes out to press 1.8bar；
(5) microwave-assisted is combined enzyme treatment process: the Folium Mori filtering residue that will filter out puts into extraction pot again,
Add 5 times of weight compound enzyme aqueous solution, enzymolysis and extraction temperature 50 C, pH 5.5, time 6h, wherein
The proportioning of compound enzymic preparation is calculated by mass is: cellulase 60%, pectase 20%, xylanase 20%；
Wherein the average enzyme of compound enzymic preparation is lived as 10000-50000 unit；
(6) extraction process for the second time: after enzymolysis completes, mends purified water to 12 times of Quality of Mulberry Leaves, is sealing
Heat while stirring under state, heat 95 DEG C, maintain 2h；
(7) filtering technique for the second time: feed liquid is cooled to room temperature, first carries out initial filter with 80 mesh stainless steel filtering nets,
Carrying out fine straining with the ceramic membrane that pore size filter is 0.5 μm again, operating condition is that temperature is less than 65 DEG C, entrance pressure
3.0bar, goes out to press 1.8bar；
The merging of (8) twice extracting solution and concentration technology: merge twice filtrate and concentrate, thickening temperature
85 DEG C, vacuum is at 0.15MPa, and being concentrated into solid content is 20%；
(9) drying process: be spray-dried being concentrated into the feed liquid that solid content is 20%, spray drying tower air intake
Temperature 140 DEG C, temperature of outgoing air is more than 75 DEG C；
(10) granulating process: do binding agent after concentrated solution after filtering with leaching, pelletizes with dried powder bonding,
Concentrated solution is 1:0.6 with the mass ratio of dried powder, and regulation compressed air pressure is spraying 0.25MPa, injection:
0.15MPa, peristaltic pump rotating speed 80r/min, < 80 DEG C, Granulation time is 70min to control temperature.
Embodiment 2:
A kind of preparation method of Folium Mori instant tea:
(1) stoving process: by fresh Folium Mori raw material roguing, 80 DEG C of air blast are dried to the 20～28% of original weight；
(2) disintegrating process: the Folium Mori after drying carry out pulverizing and sieving 20～40 mesh；
(3) extraction process for the first time: Folium Mori are put into the extraction pot of band microwave and temperature control, by people's Folium Mori 10
The purified water of times weight, heats while stirring, heats 95 DEG C, maintains 2h；
(4) filter for the first time: after having extracted, the tea after stirring leaching is starched in the leaching can sealed cold
But to room temperature, first initial filter is carried out with 60 mesh stainless steel filtering nets, then with the ceramic membrane that pore size filter is 0.5 μm
Carrying out fine straining, operating condition is that temperature is less than 65 DEG C, and entrance pressure 2.5bar goes out to press 0.2bar；
(5) microwave-assisted is combined enzyme treatment process: the Folium Mori filtering residue that will filter out puts into extraction pot again,
Add 4 times of weight compound enzyme aqueous solution, enzymolysis and extraction temperature 50 C, pH 5.0, time 4h, wherein
The proportioning of compound enzymic preparation is calculated by mass is: cellulase 60%, pectase 20%, xylanase 20%；
Wherein the average enzyme of compound enzymic preparation is lived as 10000-50000 unit；
(6) extraction process for the second time: after enzymolysis completes, mends purified water to 12 times of Quality of Mulberry Leaves, is sealing
Heat while stirring under state, heat 95 DEG C, maintain 2h；
(7) filtering technique for the second time: feed liquid is cooled to room temperature, first carries out initial filter with 80 mesh stainless steel filtering nets,
Carrying out fine straining with the ceramic membrane that pore size filter is 0.5 μm again, operating condition is that temperature is less than 65 DEG C, entrance pressure
2.5bar, goes out to press 0.2bar；
The merging of (8) twice extracting solution and concentration technology: merge twice filtrate and concentrate, thickening temperature
85 DEG C, vacuum is at 0.10MPa, and being concentrated into solid content is 18%；
(9) drying process: be spray-dried being concentrated into the feed liquid that solid content is 18%, spray drying tower air intake
Temperature 130 DEG C, temperature of outgoing air is more than 75 DEG C；
(10) granulating process: do binding agent after concentrated solution after filtering with leaching, pelletizes with dried powder bonding,
Concentrated solution is 1:0.4 with the mass ratio of dried powder, and regulation compressed air pressure is spraying 0.20MPa, injection:
0.10MPa, peristaltic pump rotating speed 70r/min, < 80 DEG C, Granulation time is 50min to control temperature.
Embodiment 3:
A kind of preparation method of Folium Mori instant tea:
(1) stoving process: by fresh Folium Mori raw material roguing, 80 DEG C of air blast are dried to the 20～28% of original weight；
(2) disintegrating process: the Folium Mori after drying carry out pulverizing and sieving 20～40 mesh；
(3) extraction process for the first time: Folium Mori are put into the extraction pot of band microwave and temperature control, by people's Folium Mori 10
The purified water of times weight, heats while stirring, heats 95 DEG C, maintains 2h；
(4) filter for the first time: after having extracted, the tea after stirring leaching is starched in the leaching can sealed cold
But to room temperature, first initial filter is carried out with 120 mesh stainless steel filtering nets, then with the ceramic membrane that pore size filter is 0.5 μm
Carrying out fine straining, operating condition is that temperature is less than 65 DEG C, and entrance pressure 3.4bar goes out to press 1.3bar；
(5) microwave-assisted is combined enzyme treatment process: the Folium Mori filtering residue that will filter out puts into extraction pot again,
Add 5 times of weight compound enzyme aqueous solution, enzymolysis and extraction temperature 55 DEG C, pH 6.0, time 4h, wherein
The proportioning of compound enzymic preparation is calculated by mass is: cellulase 60%, pectase 20%, xylanase 20%；
Wherein the average enzyme of compound enzymic preparation is lived as 10000-50000 unit；
(6) extraction process for the second time: after enzymolysis completes, mends purified water to 20 times of Quality of Mulberry Leaves, is sealing
Heat while stirring under state, heat 95 DEG C, maintain 2h；
(7) filtering technique for the second time: feed liquid is cooled to room temperature, first carries out initial filter with 80 mesh stainless steel filtering nets,
Carrying out fine straining with the ceramic membrane that pore size filter is 0.5 μm again, operating condition is that temperature is less than 65 DEG C, entrance pressure
3.4bar, goes out to press 1.3bar；
The merging of (8) twice extracting solution and concentration technology: merge twice filtrate and concentrate, thickening temperature
85 DEG C, vacuum is at 0.10MPa, and being concentrated into solid content is 30%；
(9) drying process: be spray-dried being concentrated into the feed liquid that solid content is 30%, spray drying tower air intake
Temperature 130 DEG C, temperature of outgoing air is more than 75 DEG C；
(10) granulating process: do binding agent after concentrated solution after filtering with leaching, pelletizes with dried powder bonding,
Concentrated solution is 1:0.6 with the mass ratio of dried powder, and regulation compressed air pressure is spraying 0.20MPa, injection:
0.02MPa, peristaltic pump rotating speed 80r/min, < 80 DEG C, Granulation time is 90min to control temperature.
Comparative example 1:
Choose new fresh mulberry leaf, use following methods to be prepared Folium Mori instant tea, contrast with embodiment 1,
Remaining technique is identical, and the microwave-assisted decreasing step (5) is combined enzyme treatment process.
The Folium Mori instant tea finished product that Example 1 and comparative example 1 obtain carries out gamma aminobutyric acid content and 1-takes off
Oxygen nojirimycin content measuring, compares:
Wherein, gamma aminobutyric acid detection method of content uses high effective liquid chromatography for measuring: chromatographic condition uses
C18 column chromatography post (250mm × 4.6mm), with 4.1g/L sodium-acetate buffer-acetonitrile (95:5) for flowing phase,
Detection wavelength is 254nm, takes 0.01, and 0.02,0.05,0.10,0.50,1.03g/L reference substance solution is entered
Sample detects, and draws drawbacks of the standard, and its equation of linear regression is Y=1.6 × 106X-1.1 × 105, R2=0.9996.
1-DNJ content detection employing chromatographic column: C18 analytical column (5 μm, 250mm × 4.6mm)
And ODS3 pre-column (5 μm, 10mm × 4.6mm)；Flowing phase: acetonitrile-0.1% acetic acid (55:45, V/V)；
Flow velocity: 1.0ml/min；Column temperature 25 DEG C；Fluorescence detector excitation wavelength 254nm, launches wavelength 322nm；
Sample size 10 μ l.Equation Y=3204.4+36892X, coefficient R=0.9999.
Test result is as shown in table 1:
Table 1 embodiment 1 and both effectiveness component content contrast in comparative example 1 product
| Contrast index | Embodiment 1 | Comparative example 1 | Content increase rate |
| Gamma aminobutyric acid content (mg/g) | 28.5 | 20.7 | 37.7% |
| 1-DNJ content (mg/g) | 42.6 | 28.2 | 51.1% |
From table 1 data it can clearly be seen that the product of embodiment 1 gamma aminobutyric acid content and 1-deoxidation wild
The mould cellulose content of buttocks is significantly more than comparative example 1, and gamma aminobutyric acid content improves 37.7%, 1-DNJ
Content improves 51.1%.
Embodiment 4:
The application of a kind of Folium Mori instant tea: application three-hypers crowd and normal population blood fat reducing, blood sugar lowering, blood pressure lowering
Health food, there is significant health-care effect.
Carry out the proof of effect below with rat test, concrete test method is as follows:
A) blood fat reducing test
A-1) subjects:
Taking the healthy adult male rat 40 of 150～200g body weight, divide four groups, often group 10, one group is
Hyperlipidemia matched group, three groups is test group, respectively test group 1, test group 2, test group 3.
A-2) artificial blood fat raises:
Four groups of equal feedings of rat contain mass fraction be 1% cholesterol, 10% yolk powder, the feedstuff 7 of 10% Adeps Sus domestica～
10 days so that it is serum total cholesterol values reaches more than 7.0mmol/L, serum triglycerides all reached 2.0mmol/L
Above.
A-3) Folium Mori instant tea feeding:
Three groups of rats of test, test group 1 per os gavage, every day feeding 4g embodiment 1 Folium Mori instant tea
Granule, 2 groups of per os gavages of test group, every day feeding 3g embodiment 1 preparation Folium Mori instant tea granule,
Test group 3 per os gavage, every day feeding 2g embodiment 1 Folium Mori instant tea granule, hyperlipidemia matched group is big
Mus per os gavage, every day feeding 5g water, four groups of rats all continue feeding with front identical feedstuff, test 30 altogether
My god.
A-4) result of the test:
After measured, comparing with hyperlipidemia matched group, test group 1 rat blood serum T-CHOL declines average out to
18.2%, serum levels of triglyceride declines average out to 21.2%；Test group 2 rat blood serum T-CHOL declines average
Being 15.1%, serum levels of triglyceride declines average out to 18.3%, and test group 3 rat blood serum T-CHOL declines flat
Being 11.5%, serum levels of triglyceride declines average out to 14.9%；Data show: the Folium Mori of embodiment 1 preparation
Instant tea has obvious lipid-lowering effect to rat.
B) blood sugar lowering test
B-1) subjects:
Taking the healthy adult male rat 40 of 150～200g body weight, divide four groups, often group 10, one group is
Hyperglycemia matched group, three groups is test group, respectively test group 1, test group 2, test group 3.
B-2) artificial blood glucose raises:
After fasting 24 hours, by the amount of 60 μ g/g body weight, intravenous injection alloxan, normal diet is fed
Supporting 5～7 days, fasting 5h surveys blood glucose, it is desirable to blood glucose value reaches 15～20mmol/L.
B-3) Folium Mori instant tea feeding:
Test group 1 per os gavage, every day feeding 4g embodiment 1 Folium Mori instant tea granule, test group 2 warp
Mouthful gavage, every day feeding 3g embodiment 1 Folium Mori instant tea granule, test group 3 per os gavage, feed every day
The Folium Mori instant tea granule of food 2g embodiment 1；Hyperglycemia control rats per os gavage, every day feeding 5g
Water, four groups of rats all continue feeding with front identical feedstuff, long run test 30 days.
B-4) result of the test:
After measured, comparing with hyperglycemia matched group, test group 1 rat fasting glucose after fasting 5 hours declines
Value average out to 17.2%, test group 2 rat is fasting glucose drop-out value average out to 13.2% after fasting 5 hours,
Test group 3 rat is fasting glucose drop-out value average out to 9.8% after fasting 5 hours.Four groups of equal fasting of rat 5
Hour, three groups of rats of test and hyperglycemia control rats all by 2.0mg/g body weight per os gavage glucose,
2 as a child measured blood glucose value, and result test group 1 rat is lower than hyperglycemia matched group by 16.5%, test group
2 rats are lower than hyperglycemia matched group by 14.2%, and test group 3 rat is lower than hyperglycemia matched group
12.2%, result proves that the Folium Mori instant tea of the present invention has good hypoglycemic effect.
C) blood pressure lowering test
C-1) subjects:
Take spontaneous hypertensive rat 40, male and female half and half, body weight 180～220g, measuring blood pressure and heart rate, divide
Four groups, often group 10.First group is hypertension matched group, and remaining is test group 1, test group 2, test group
3。
C-2) Folium Mori instant tea feeding:
Test group 1 per os gavage, every day feeding 4g embodiment 1 Folium Mori instant tea granule, test group 2 warp
Mouthful gavage, every day feeding 3g embodiment 1 Folium Mori instant tea granule, test group 3 per os gavage, feed every day
The Folium Mori instant tea granule of food 2g embodiment 1；Hypertension control rats per os gavage, every day feeding 5g
Water, four groups of rats all continue feeding and front identical feedstuff, long run test 30 days；During test weekly measuring blood pressure,
Heart rate twice, off-test, measuring blood pressure and heart rate 3～5 times in 10 days；Separately take normal rat 30～40,
Requirement same as described above, points three groups, per os feeding product, three groups every day feeding embodiment 1 Folium Mori instant tea
Granule is still one group of 4g, one group of 3g, one group of 2g, and continues feeding and front identical feedstuff, continuous 30 days.
C-3 result of the test:
By comparative measurements result, product on spontaneous hypertensive rat heart rate without impact, the blood to normal rat
Pressure and heart rate are without impact.Spontaneous hypertension rat test group 1 averagely reduces 17.2mmHg than hypertension matched group,
Spontaneous hypertensive rat test group 2 averagely reduces 13.2mmHg than hypertension matched group, spontaneously hypertensive
Rat test group 3 averagely reduces 10.1mmHg than hypertension matched group.Result proves that extract of the present invention has
Good blood pressure reduction effect.
In addition testing embodiment 2, embodiment 3 according to above-mentioned same test method, blood fat reducing is tested
The results are shown in Table 2, blood sugar lowering result of the test is shown in Table 3, blood pressure lowering result of the test is shown in Table 4:
Table 2: rats in test groups is serum total cholesterol/serum triglycerides down ratio compared with hyperlipidemia matched group
Table 3: after rats in test groups fasting 5h compared with matched group blood glucose decrease/fasting 5h, rats in test groups with compare
Compare blood glucose after organizing equal per os gavage glucose 2h and reduce ratio
Table 4: spontaneously hypertensive rats in test groups is drop-out value of blood pressure (mmHg) compared with matched group
The data of table 2-4 it can clearly be seen that the Folium Mori instant tea granule of the present invention have good blood fat reducing,
Blood sugar lowering, effect of blood pressure lowering, and effect is substantially better than Folium Mori tea granule prepared by common process, can be as three
High crowd and the health food of normal person.
It should be pointed out that, that above detailed description of the invention is merely to illustrate the present invention rather than limits the model of the present invention
Enclosing, after having read the present invention, the amendment of the various equivalent form of values of the present invention is all fallen by those skilled in the art
In the application claims limited range.
Claims (8)
1. the preparation method of a Folium Mori instant tea, it is characterised in that preparing raw material is new fresh mulberry leaf, specifically wraps
Include following steps: stoving process, disintegrating process, for the first time extraction process, for the first time filtering technique, microwave are auxiliary
Help compound enzyme treatment process, for the second time extraction process, for the second time filtering technique, the merging of twice extracting solution and dense
Contracting technique, drying process and granulating process；Wherein twice extraction process all uses Quality of Mulberry Leaves 10～20 times
Purified water at a temperature of 95 DEG C, extract 2h, described microwave-assisted compound enzyme treatment process condition is microwave merit
Rate 300～1200W, temperature 35～55 DEG C, pH 5.0～6.0, the time 4～10h.
The preparation method of Folium Mori instant tea the most according to claim 1, it is characterised in that:
Described stoving process is: by fresh Folium Mori raw material roguing, 80 DEG C of air blast are dried to the 20～28% of original weight；
Described disintegrating process is: the Folium Mori after drying carry out pulverizing 20～40 mesh sieves；
Described first time extraction process is: put into leaching in the ratio that mass ratio is 1:10～1:20 of Folium Mori Yu water
Take in tank, heat the most while stirring, heat 95 DEG C, maintain 2h hour；
Described first time filtering technique is: in the leaching can sealed, the extracting solution after stirring leaching is cooled to room
Temperature, first carries out initial filter with 60～120 mesh stainless steel filtering nets, then enters with the ceramic membrane that pore size filter is 0.5 μm
Row fine straining, operating condition is that temperature is less than 65 DEG C, entrance pressure 2.5～3.4bar, goes out pressure 0.2～1.3bar；
Described microwave-assisted is combined enzyme treatment process: will filter after Folium Mori filtering residue add Folium Mori dry weight 4～
In the compound enzyme aqueous solution of 6 times, control extraction pot microwave power 300～1200W, temperature 35～55 DEG C, pH
5.0～6.0, the time 4～10h；
Described second time extraction process is: microwave-assisted compound enzyme processes the solution after PROCESS FOR TREATMENT and mends purified water
To 10～20 times of Quality of Mulberry Leaves, heat the most while stirring, heat 95 DEG C, maintain 2h；
Described second time filtering technique is with filtering technique for the first time；
The merging technique of described twice extracting solution and concentration technology be: merges twice filtrate and concentrates, and concentrates
Temperature 80～90 DEG C, vacuum is 0.10～0.02MPa, and being concentrated into solid content is 18～30%；
Described drying process is: be spray-dried by concentrated solution, spray drying tower inlet temperature 130 DEG C～150 DEG C,
Temperature of outgoing air is more than 75 DEG C；
Described granulating process is: do binding agent after concentrated solution after filtering with leaching, pelletizes with dried powder bonding,
The mass ratio of concentrated solution and dried powder is 1:0.4～1:0.8, regulation compressed air pressure for spraying 0.2～
0.3MPa, injection: 0.1～0.2MPa, peristaltic pump rotating speed 70～90r/min,
The grain time is 50～90min.
The preparation method of Folium Mori instant tea the most according to claim 2, it is characterised in that: described microwave
In assisted recombination enzyme treatment process, pH regulator uses citric acid.
The preparation method of Folium Mori instant tea the most according to claim 2, it is characterised in that: described compound
In enzyme aqueous solution, compound enzymic preparation addition is the 0.2%～0.9% of Folium Mori dry weight.
The preparation method of Folium Mori instant tea the most according to claim 4, it is characterised in that: described compound
The proportioning of enzyme preparation is calculated by mass is: cellulase 50%～80%, pectase 15%～30%, the wood of surplus
Dextranase.
The preparation method of Folium Mori instant tea the most according to claim 4, it is characterised in that: described compound
The average enzyme of enzyme preparation is lived as 10000-50000 unit.
The preparation method of Folium Mori instant tea the most according to claim 1, it is characterised in that: described microwave
Assisted recombination enzyme treatment process condition is temperature 45 C, pH 5.5, time 6h.
8. the application of Folium Mori instant tea as claimed in claim 1, it is characterised in that be applied to three-hypers crowd and
Normal population blood fat reducing, blood sugar lowering, the health food of blood pressure lowering.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610348102.1A CN105994791A (en) | 2016-05-24 | 2016-05-24 | Preparation method and application of mulberry leaf instant tea |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610348102.1A CN105994791A (en) | 2016-05-24 | 2016-05-24 | Preparation method and application of mulberry leaf instant tea |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105994791A true CN105994791A (en) | 2016-10-12 |
Family
ID=57094748
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610348102.1A Pending CN105994791A (en) | 2016-05-24 | 2016-05-24 | Preparation method and application of mulberry leaf instant tea |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105994791A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107048373A (en) * | 2017-05-31 | 2017-08-18 | 江苏鑫缘丝绸科技有限公司 | A kind of ultra-fine mulberry leaf powder and preparation method thereof |
| CN107296127A (en) * | 2017-06-30 | 2017-10-27 | 阜阳市辉源果桑科技产业园有限公司 | A kind of preparation method of mulberry leaf powder tea |
| CN108185081A (en) * | 2018-02-28 | 2018-06-22 | 贵州省怡丰原生态茶业发展有限责任公司 | A kind of processing method of instant mulberry leaf tea powder |
| CN113854380A (en) * | 2021-10-09 | 2021-12-31 | 甘肃省商业科技研究所有限公司 | Instant solid tea prepared from pure medicinal and edible plant raw materials and its preparation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101189998A (en) * | 2006-11-28 | 2008-06-04 | 大闽食品（漳州）有限公司 | Method for processing cold soluble type instant tea |
| CN104336230A (en) * | 2013-07-27 | 2015-02-11 | 卢柳 | Mulberry leaf sweet tea and preparation method thereof |
| CN104431065A (en) * | 2014-11-17 | 2015-03-25 | 江西省蚕桑茶叶研究所 | Preparation method of mulberry leaf oolong tea |
| CN105285225A (en) * | 2015-11-23 | 2016-02-03 | 湖南梅山黑茶股份有限公司 | Preparation method of instant tea and instant tea thereof |
| CN105494765A (en) * | 2016-01-11 | 2016-04-20 | 福建省农业科学院茶叶研究所 | Instant tea and preparing method thereof |
-
2016
- 2016-05-24 CN CN201610348102.1A patent/CN105994791A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101189998A (en) * | 2006-11-28 | 2008-06-04 | 大闽食品（漳州）有限公司 | Method for processing cold soluble type instant tea |
| CN104336230A (en) * | 2013-07-27 | 2015-02-11 | 卢柳 | Mulberry leaf sweet tea and preparation method thereof |
| CN104431065A (en) * | 2014-11-17 | 2015-03-25 | 江西省蚕桑茶叶研究所 | Preparation method of mulberry leaf oolong tea |
| CN105285225A (en) * | 2015-11-23 | 2016-02-03 | 湖南梅山黑茶股份有限公司 | Preparation method of instant tea and instant tea thereof |
| CN105494765A (en) * | 2016-01-11 | 2016-04-20 | 福建省农业科学院茶叶研究所 | Instant tea and preparing method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 刘宝家等: "《食品加工技术、工艺和配方大全 下册》", 28 February 1990, 科学技术文献出版社 * |
| 汪世龙等: "《蛋白质化学》", 31 August 2012, 同济大学出版社 * |
| 王芳等: ""桑叶多糖的提取工艺研究"", 《浙江师范大学学报（自然科学版）》 * |
| 陈忠立等: ""桑叶速溶茶的研制"", 《丝绸》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107048373A (en) * | 2017-05-31 | 2017-08-18 | 江苏鑫缘丝绸科技有限公司 | A kind of ultra-fine mulberry leaf powder and preparation method thereof |
| CN107296127A (en) * | 2017-06-30 | 2017-10-27 | 阜阳市辉源果桑科技产业园有限公司 | A kind of preparation method of mulberry leaf powder tea |
| CN108185081A (en) * | 2018-02-28 | 2018-06-22 | 贵州省怡丰原生态茶业发展有限责任公司 | A kind of processing method of instant mulberry leaf tea powder |
| CN113854380A (en) * | 2021-10-09 | 2021-12-31 | 甘肃省商业科技研究所有限公司 | Instant solid tea prepared from pure medicinal and edible plant raw materials and its preparation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP3141255B1 (en) | Memory improving composition, preparation method and use thereof | |
| CN101791119B (en) | Chaga nutrient composition | |
| CN105994791A (en) | Preparation method and application of mulberry leaf instant tea | |
| CN101412703A (en) | Composite extracting technique for coproduction of mulberry tea flavone, polysaccharide and alkaloid | |
| CN107412721B (en) | Blood sugar-reducing bitter gourd polypeptide compound capsule and preparation method thereof | |
| CN104473171A (en) | Cannabis sativa buccal tablet and preparation method thereof | |
| CN104131057A (en) | Functional white kidney bean polypeptide and preparation method and application thereof | |
| CN105622775A (en) | Nostoc sphaeroides polysaccharide and extracting method thereof | |
| CN105077232A (en) | Dendrobium officinale and fructus lycii capsule and preparation method thereof | |
| CN106720752A (en) | Camellia chrysantha beverage and preparation method thereof | |
| CN104258193A (en) | Drug composition for treating neurasthenia and preventing senile dementia | |
| CN111019984A (en) | Efficient method for extracting sialic acid from cubilose | |
| CN111249338A (en) | Cistanche deserticola extract and industrial preparation method and application thereof | |
| CN105294881B (en) | A kind of method that tree peony Fruit pod Thick many candies are extracted in the Fruit pod from tree peony | |
| CN105943569A (en) | Method for extracting effective ingredients of green plums | |
| CN105031289A (en) | Dendrobium officinale and lucid ganoderma capsules and preparation method thereof | |
| CN101709076A (en) | Method for preparing hoof nail polypeptide | |
| CN107674902B (en) | Camel blood polypeptide with blood sugar reducing function and preparation method thereof | |
| CN105367433A (en) | Method for extracting gamma-aminobutyric acid from barley germs | |
| CN105532350B (en) | A kind of preparation method of hainan holly leaf ginkgo biloba extract compound tea | |
| CN106860489A (en) | A kind of extracting method of myrica rubra leaf polyphenol | |
| CN112641826B (en) | Perilla seed extract and preparation method and application thereof | |
| CN105412613B (en) | A kind of formula and its processing method for senile dementia | |
| CN105560173B (en) | A kind of high stability astragalus injection and preparation method thereof | |
| CN103815399A (en) | Processing method of Semen Celosiae-Antrodia camphorate nutrition powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20170524 Address after: 222000 room 17-1-801, room 816, flower mountain road, Haizhou District, Jiangsu, Lianyungang Applicant after: Jiangsu NANDA bird Biotechnology Co., Ltd. Address before: Zhenjiang City, Jiangsu province 212415 Xianlin Baohua town of Jurong City Road No. 16 Applicant before: JIANGSU NAIQUE BIOLOGICAL ENGINEERING TECHNOLOGY CO., LTD. |
|
| TA01 | Transfer of patent application right | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161012 |
|
| RJ01 | Rejection of invention patent application after publication |