CN106723040A - A kind of lotus root polyphenol polysaccharide-liposome complex liquid and preparation method thereof - Google Patents
A kind of lotus root polyphenol polysaccharide-liposome complex liquid and preparation method thereof Download PDFInfo
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- 235000006508 Nelumbo nucifera Nutrition 0.000 title claims abstract description 112
- 239000002502 liposome Substances 0.000 title claims abstract description 62
- 239000007788 liquid Substances 0.000 title claims abstract description 48
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 37
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 150000004676 glycans Chemical class 0.000 claims abstract description 40
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 40
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- 238000000034 method Methods 0.000 claims abstract description 21
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to a kind of lotus root polyphenol polysaccharide-liposome complex liquid and preparation method thereof, the method comprises the following steps：Lotus root polyphenol and lipoid prepare mixed liquor, be that solvent prepares the mixing of lotus root polysaccharide solution, mixed liquor and lotus root polysaccharide solution simultaneously ice-bath ultrasonic obtains w/o type emulsion, rotates to obtain suspension, high-pressure homogeneous with PBS with absolute ethyl alcohol as solvent, obtains liposome complex liquid.Beneficial effects of the present invention are：Lotus root polyphenol and polysaccharide are prepared into liposome complex liquid first, are the intensive processing to lotus rhizome and its processing byproduct, the liposome complex liquid of preparation can effectively strengthen the application benefit of lotus rhizome functional product, increased added value；The liposome complex liquid not only solves that lotus rhizome active material stability is poor, the low problem of bioavailability, and realizes the complementation and synergy of the biological effect of active material.
Description
Technical field
The invention belongs to functional food processing technique field, more particularly to a kind of lotus root polyphenol-polysaccharide-liposome complex liquid
And preparation method thereof.
Background technology
Lotus rhizome is China's cultivation most wide, sales volume and the maximum aquatic vegetable of sales range.Because of seasonal strong, Time To Market
Relatively centralized, lotus rhizome major part marketing fresh conversion in the form of primary agricultural products is larger by market supply and demand influence of fluctuations, easily produces
" dish is low-priced to hinder agriculture " phenomenon, influences the basic of industry development.Additionally, lotus rhizome easily brown stain and corruption during storage, fortune, pin etc.,
Existing quality keeps that technology is still difficult to meet low cost, high safety and the multiple requirements such as long-lasting, loses serious after causing to adopt,
Greatly develop the important leverage that processing industry is Lotus Industry stable development.Currently, the processing of lotus rhizome is broadly divided into two class modes：Its
One be cutting fabricated product, such as fresh class, pickle class, dehydration class, quick-frozen class, cooking class；The second is content fabricated product,
Such as lotus root juice beverage, instant lotus root starch, lotus root wine.Generally speaking, lotus rhizome processing scientific and technological level is weak, and product " homogeneity " phenomenon is tight
Weight.Additionally, producing the accessory substances such as substantial amounts of lotus root skin, rhizoma nelumbinis and lotus root slag in process, effective exploitation utilization is not obtained, cause
The waste of resource and the pollution of environment.The creative utilization of Nelumbo is probably the key of industry sustainable development, and plays money
The characteristic and advantage in source are then important points of penetration.
Lotus rhizome as a kind of extremely common vegetables, the effects such as its heat-clearing, hemostasis, regulation endocrine, nourishing are calmed the nerves already
It is well known, and in ancient books《Bencao Jingshu》It is expressly recited with pharmacopeia.Modern pharmacology research finds, in lotus rhizome
Main active is polyphenol and SNSP, shows anti-oxidant, hypoglycemic, immunological regulation, improves the health care of sleep and remember
Various biological effects.Active component content enriches in lotus rhizome, and polyphenol and SNSP account for 0.13% He of its fresh weight
2.5%.The good market prospect of natural activity polyphenol and polysaccharide in fields such as food, medicine and cosmetics.Therefore, active polyphenol and
The extraction exploitation of polysaccharide is probably the important point of penetration of Nelumbo creative utilization.The correlative study present situation of lotus root polyphenol and polysaccharide
It is summarized as follows：
(1) lotus root polyphenol present Research.Kaur etc. (2002) uses beta carotene/linoleic acid emulsion method research to find,
Lotus rhizome has stronger oxidation resistance in 36 kinds of Asia vegetables.Guo Changjiang etc. (2003) is using FRAP methods to 36 kinds of Vegetables
Antioxidant assay is carried out, it is found that the oxidation resistance of lotus root is most strong, be significantly better than ginger, rape, cowpea, taro etc..Hereafter, Yang Dongmei
(2007) synthesis FRAP methods, DPPH methods, beta carotene/linoleic acid emulsion method compare common including 12 kinds including lotus rhizome
The antioxidation activity of vegetables, and obtain similar conclusion.And the oxidation resistance of lotus rhizome is directly proportional to its content of phenolic compounds
(Hu,et al,2002).Lot of documents research report confirms that lotus root polyphenol has good free radical scavenging activity, total antioxygen
Change ability and lipid-antioxidant activity ability (Yi, 2016；Maisuthisakul,2007；Xu Yanyan, 2015；Qin Haiming, 2015；Liu
Shining cloud, 2011；Guard thunder, 2005 rigidly).Except antioxygen is outside the pale of civilization, lotus root polyphenol also show staphylococcus aureus suppress (guard thunder rigidly,
2006), hypoglycemic (Mukherjee, 1997), enhancing memory and neurodevelopment (Yang, 2008；Mukherjee, 1996) etc.
Effect.
(2) lotus rhizome SNSP present Research.SNSP accounts for the 2.5% of its dry weight, wherein molecule in lotus rhizome
Acidic components of the amount more than 2000kDa are mainly made up of galactolipin, arabinose, rhamnose, glucose, fucose and xylose
(Yan Lang, 2007)；Molecular weight is mainly made up of for the component of 18.8kDa mannose, rhamnose, glucose, galactolipin and xylose
(Jiang Jun, 2010).Antioxidation in vitro evaluation finds, lotus rhizome SNSP can effective scavenging hydroxyl, and suppress H2O2 and lure
Oxidative hemolysis of erythrocyte (Wang Yu, 2007 for leading；Yan Lang, 2008).Lotus rhizome water extract can also significantly increase the body fluid of experimental animal
With cellular immune function (Mukherjee, 2010), and these effects may be closely related with SNSP.Lotus root polysaccharide LB2
The expression of Balb/c Turnover of Mouse Peritoneal Macrophages IL-2, IL-4 and IL-10 can be raised, body immune system is activated, is conducive to changing
The state that the kind HIV-1 infected cell factors are generally reduced, maintains immunity of organism balance, and the downward that LB2 is expressed TNF-α to make
With the ability (Jiang Jun, 2010) for making it have directly suppression HIV-1 duplications.Additionally, lotus root polysaccharide effectively to reduce diabetes small
Mouse blood sugar, improves glucose tolerance, and improves liver, kidney and SOD activity in pancreas to some extent, reduces MDA contents；Table
Reveal significant anti-fatigue active, the mice burden swimming time can be extended, reduce blood lactase acid, the content of serum urea nitrogen, improve
Hepatic glycogen content (Luo Denghong, 2011；Zhou Taoying, 2011).
At present, it is rarely seen for the extraction preparation and research and development of products of the application and development of lotus root functional product, especially active component
Report.Zhou Taoying et al. (2011) have developed a kind of antifatigue health drink of lotus root polysaccharide；Applicant (2015) have developed one
Plant lotus root polyphenol oil antioxidant；It is fine that Gu Zhenxin (2009), Li Jie (2013), Wang Honggang (2015) etc. have developed lotus rhizome meals
Dimension series of products；Wu Mengdan (2015) have developed the water plant compound with antitumor efficacy containing lotus root composition；Li Jing
(2015) hypertension containing lotus root composition, diabetes and gastric ulcer series auxiliary treatment drink be have developed.Following table is summary and induction
The lotus root product and lotus root composition addition product with functional activity reported in the prior art：
The document and Patents be given in analysis upper table understand that the activity of lotus root polyphenol and polysaccharide has similar, also has
It is different, thus be contemplated that lotus root polyphenol and polysaccharide are prepared into compound together, both different activity can with cross complementary, two
Identical activity superposition can play synergy work to be had.
Liposome is typical spherical structure, the amphiphilic film with individual layer or multilayer, can be used to encapsulate, transports
Carry and sustained release is water-soluble, oil-soluble and amphiphilic species, the features such as with targeting, hypotoxicity, protectiveness, slow release.When
Before, the research and development object of plant polyphenol lipoid plastid is mainly pomegranate peel polyphenol (Li Jianke, CN102204939A) and Tea Polyphenols (Liu
Big, CN103637989A), and the research and development object of plant polyose lipoid plastid be related to licorice polysaccharide (Wu Yi, CN105012239A),
Ophiopogonpolysaccharide (Fan Yunpeng, CN104490783A), Dihuang polysaccharide (Wang Deyun, CN103536534A), Phellinus polysaccharide (Teng Lirong,
CN102631319A), Cordyceps sinensis polysaccharide (Teng Lirong, CN101401789) etc..Liposome preparation on lotus root polyphenol and polysaccharide
There is not been reported, and is also rarely reported for the preparation of polyphenol-polysaccharide-liposome complex liquid.
The problem that existing correlation technique is present is summarized as at following 5 points：
(1) the process technology level of lotus rhizome is weak, and product " homogeneity " is serious, and comprehensive utilization degree is poor, not fully
Using the characteristic and advantage of resource.
(2) lotus root polyphenol and polysaccharide show good bioactivity, and related application technology research and development are only limitted to carry substantially
Taking technique, Technology Chain is shorter, can not effectively support the intensive processing of lotus rhizome and its processing byproduct.
(3) polyphenol stability is poor, the influence of the factor such as easy light, heat, oxygen, easily loses physiological function, many on lotus rhizome
Phenol stable state technology (including lotus root polyphenol liposome preparation) has no report.
(4) liposome can improve polyoses oral bioavilability problem on the weak side, and lotus root polysaccharide liposome preparatory techniques
Also report is had no.
(5) biological effect of lotus root polyphenol and polysaccharide has intersection and complementation, and preparing its liposome complex liquid can be effective
Strengthen the application benefit of product, but be related to polyphenol-polysaccharide-liposome complex liquid technology of preparing to be still rarely reported.
The content of the invention
For many deficiencies of the prior art, the technical problems to be solved by the invention be to provide a kind of lotus root polyphenol-
Polysaccharide-liposome complex liquid and preparation method thereof, lotus root polyphenol and polysaccharide are prepared into liposome complex liquid, not only solved by it
Active material stability is poor, the low problem of bioavailability, and realizes the complementation and synergy of the biological effect of active material.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：A kind of lotus root polyphenol-polysaccharide-liposome complex liquid
Preparation method, it comprises the following steps：
1) it is the lotus root polyphenol solution that solvent compound concentration is 0.5~2.5mg/mL with absolute ethyl alcohol, is mixed after adding lipoid
Conjunction forms mixed liquor；
2) the use of PBS is the lotus root polysaccharide solution that solvent prepares that mass fraction is 0.5~1.5%, by step 1)
Mixed liquor mix with lotus root polysaccharide solution, be placed in ice-water bath it is ultrasonically treated formation w/o type emulsion；
3) by step 2) in w/o type emulsion add rotary evaporation bottle in, the vacuum rotary steam in 45~55 DEG C of water-baths treats shape
PBS is added after removing into colloidal state and completely ethanol, and 60~120min of degumming is rotated in 45 DEG C of water-baths, obtain liposome
Suspension；
4) by step 3) in liposome turbid liquor in high pressure homogenizer circular treatment, obtain final product the lotus root polyphenol-many
Glycolipid plastid complex liquid.
Specifically, step 1) described in lipoid be mass ratio be 4~10：1 soybean lecithin and cholesterol.Preferably 6~
8：1.
Specifically, step 1) described in mixed liquor the mass fraction of lipoid be 5~15%.
Specifically, step 2) in the mixed volume ratio of mixed liquor and lotus root polysaccharide solution be 3~5:1.
Specifically, step 2) in ultrasonically treated power be 300~600W, the time is 5~10min..
Specifically, step 3) in PBS additions be 0.5~1.5 times of emulsion volume..
Specifically, step 4) in suspension circular treatment in high pressure homogenizer refer to the circular treatment under 100bar pressure
4~8 times.
The present invention also provides a kind of lotus root polyphenol-polysaccharide-liposome complex liquid, and it is prepared by above-mentioned method.
Compared with prior art, the beneficial effects of the invention are as follows：Lotus root polyphenol and polysaccharide are prepared into liposome first to answer
Liquid is closed, is the intensive processing to lotus rhizome and its processing byproduct, the liposome complex liquid of preparation can effectively strengthen lotus rhizome function product
The application benefit of product, increased added value；The liposome complex liquid not only contributes to improve lotus rhizome active material stability and increase
Plus its bioavailability, and realize the complementation and synergy of the biological effect of active material.
Brief description of the drawings
The preparation technology flow chart of lotus root polyphenol-polysaccharide-liposome complex liquid that Fig. 1 is provided for the present invention；
Fig. 2 is soybean lecithin and influence of the cholesterol mass ratio to lotus root polyphenol and polysaccharide envelop rate；
Fig. 3 is the grain size distribution of lotus root polyphenol-polysaccharide-liposome complex liquid prepared by embodiment 1.
Specific embodiment
Below in conjunction with drawings and the specific embodiments, the present invention is described in further detail, and example is served only for explaining
The present invention, is not intended to limit the scope of the present invention.
Following examples prepare the technological process used during lotus root polyphenol-polysaccharide-liposome complex liquid as shown in figure 1, each
The particular content of embodiment is as follows.
Embodiment 1
A kind of lotus root polyphenol-polysaccharide-liposome complex liquid, it is prepared via a method which：
1) the lotus root polyphenol solution for being solvent compound concentration as 0.5mg/mL with absolute ethyl alcohol, takes soybean lecithin and cholesterol
Above-mentioned solution is added to form mixed liquor, soybean lecithin is 4 with the mass ratio of cholesterol：1, soybean lecithin and cholesterol in mixed liquor
Total mass fraction is 5%；
2) the use of PBS is the lotus root polysaccharide solution that solvent prepares that mass fraction is 0.5%, according to 3：1 volume
Than by step 1) mixed liquor mix with lotus root polysaccharide solution, be placed in ultrasonically treated formation w/o type emulsion, ultrasonic work(in ice-water bath
Rate is 600W, and ultrasonic time is 5min；
3) by step 2) in w/o type emulsion add rotary evaporation bottle in, the vacuum rotary steam in 55 DEG C of water-baths, glue to be formed
Added and the isometric PBS of emulsion after state and completely removing ethanol, and degumming 90min is rotated in 45 DEG C of water-baths, obtained
Liposome turbid liquor；
4) by step 3) in liposome turbid liquor in high pressure homogenizer with 100bar pressure cycles process 4 times, obtain final product
The lotus root polyphenol-polysaccharide-liposome complex liquid.
Embodiment 2
A kind of lotus root polyphenol-polysaccharide-liposome complex liquid, it is prepared via a method which：
1) the lotus root polyphenol solution for being solvent compound concentration as 1.5mg/mL with absolute ethyl alcohol, takes soybean lecithin and cholesterol
Above-mentioned solution is added to form mixed liquor, soybean lecithin is 6 with the mass ratio of cholesterol：1, soybean lecithin and cholesterol in mixed liquor
Total mass fraction is 10%；
2) the use of PBS is the lotus root polysaccharide solution that solvent prepares that mass fraction is 1%, according to 4：1 volume ratio
By step 1) mixed liquor mix with lotus root polysaccharide solution, be placed in ice-water bath it is ultrasonically treated formation w/o type emulsion, ultrasonic power
It is 500W, ultrasonic time is 6min；
3) by step 2) in w/o type emulsion add rotary evaporation bottle in, the vacuum rotary steam in 50 DEG C of water-baths, glue to be formed
The PBS with 0.5 times of emulsion volume is added after state and completely removing ethanol, and degumming is rotated in 45 DEG C of water-baths
120min, obtains liposome turbid liquor；
4) by step 3) in liposome turbid liquor in high pressure homogenizer with 100bar pressure cycles process 6 times, obtain final product
The lotus root polyphenol-polysaccharide-liposome complex liquid.
Embodiment 3
A kind of lotus root polyphenol-polysaccharide-liposome complex liquid, it is prepared via a method which：
1) the lotus root polyphenol solution for being solvent compound concentration as 2.5mg/mL with absolute ethyl alcohol, takes soybean lecithin and cholesterol
Above-mentioned solution is added to form mixed liquor, soybean lecithin is 8 with the mass ratio of cholesterol：1, soybean lecithin and cholesterol in mixed liquor
Total mass fraction is 12%；
2) the use of PBS is the lotus root polysaccharide solution that solvent prepares that mass fraction is 1.1%, according to 4：1 volume
Than by step 1) mixed liquor mix with lotus root polysaccharide solution, be placed in ultrasonically treated formation w/o type emulsion, ultrasonic work(in ice-water bath
Rate is 400W, and ultrasonic time is 8min；
3) by step 2) in w/o type emulsion add rotary evaporation bottle in, the vacuum rotary steam in 50 DEG C of water-baths, glue to be formed
The PBSs with 1.5 times of emulsion volumes are added after state and completely removing ethanol, and degumming 60min are rotated in 45 DEG C of water-baths,
Obtain liposome turbid liquor；
4) by step 3) in liposome turbid liquor in high pressure homogenizer with 100bar pressure cycles process 7 times, obtain final product
The lotus root polyphenol-polysaccharide-liposome complex liquid.
Embodiment 4
A kind of lotus root polyphenol-polysaccharide-liposome complex liquid, it is prepared via a method which：
1) the lotus root polyphenol solution for being solvent compound concentration as 2.5mg/mL with absolute ethyl alcohol, takes soybean lecithin and cholesterol
Above-mentioned solution is added to form mixed liquor, soybean lecithin is 10 with the mass ratio of cholesterol：1, soybean lecithin and courage are solid in mixed liquor
The total mass fraction of alcohol is 15%；
2) the use of PBS is the lotus root polysaccharide solution that solvent prepares that mass fraction is 1.5%, according to 5：1 volume
Than by step 1) mixed liquor mix with lotus root polysaccharide solution, be placed in ultrasonically treated formation w/o type emulsion, ultrasonic work(in ice-water bath
Rate is 300W, and ultrasonic time is 10min；
3) by step 2) in w/o type emulsion add rotary evaporation bottle in, the vacuum rotary steam in 50 DEG C of water-baths, glue to be formed
The PBSs with 1.5 times of emulsion volumes are added after state and completely removing ethanol, and degumming 60min are rotated in 45 DEG C of water-baths,
Obtain liposome turbid liquor；
4) by step 3) in liposome turbid liquor in high pressure homogenizer with 100bar pressure cycles process 8 times, obtain final product
The lotus root polyphenol-polysaccharide-liposome complex liquid.
Comparative example 1
Essentially the same with embodiment 1, difference is only soybean lecithin and the mass ratio of cholesterol is 2：1.
Respectively with embodiment 1 to 4 and the liposome complex liquid of the preparation of comparative example 1 as test object, in the liposome for measuring
It is as shown in Figure 2 to polyphenol and polysaccharide envelop rate respectively.Lotus root polyphenol-polysaccharide-liposome that the present invention is provided as seen from the figure is multiple
Lotus root polyphenol and lotus root polysaccharide have obtained preferable encapsulating in conjunction liquid, close to 80% during the former envelop rate highest, the latter's
Close to 65% during envelop rate highest, wherein with embodiment 2 (6：1) with embodiment 3 (8：1) prepare liposome complex liquid quality most
Good, it has envelop rate higher to lotus root polyphenol and lotus root polysaccharide simultaneously, and envelop rate is higher, and liposome complex liquid is more stable
(not oxidizable, precipitation etc.).The mass ratio of soybean lecithin and cholesterol is 4~10 in the preparation method that the present invention is provided：When 1,
The quality of the liposome complex liquid for obtaining is obviously improved, and the ratio is 2 in comparative example 1：1, it can be seen in fig. 2 that it is to polyphenol
Envelop rate less than 60%, to the envelop rate of polysaccharide less than 40%.
The particle diameter of the lotus root polyphenol obtained to embodiment 1 to 4-polysaccharide-liposome complex liquid is tested, and what is measured is average
Particle diameter is followed successively by 394.8nm, 359.3nm, 337.9nm, 322.5nm, wherein the liposome complex liquid particle diameter prepared to embodiment 3
Test as shown in figure 3, the average grain diameter for measuring as seen from the figure be 337.9nm.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all it is of the invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.
Claims (8)
1. the preparation method of a kind of lotus root polyphenol-polysaccharide-liposome complex liquid, it is characterised in that comprise the following steps：
1) it is the lotus root polyphenol solution that solvent compound concentration is 0.5~2.5mg/mL with absolute ethyl alcohol, shape is mixed after adding lipoid
Into mixed liquor；
2) the use of PBS is the lotus root polysaccharide solution that solvent prepares that mass fraction is 0.5~1.5%, by step 1) it is mixed
Close liquid to mix with lotus root polysaccharide solution, be placed in ultrasonically treated formation w/o type emulsion in ice-water bath；
3) by step 2) in w/o type emulsion add rotary evaporation bottle in, the vacuum rotary steam in 45~55 DEG C of water-baths, glue to be formed
PBS is added after state and completely removing ethanol, and 60~120min of degumming is rotated in 45 DEG C of water-baths, obtain liposome suspension
Liquid；
4) by step 3) in liposome turbid liquor in high pressure homogenizer circular treatment, obtain final product the lotus root polyphenol-saccharose lipid
Plastid complex liquid.
2. the preparation method of a kind of lotus root polyphenol-polysaccharide-liposome complex liquid according to claim 1, it is characterised in that
Step 1) described in lipoid be mass ratio be 4~10：1 soybean lecithin and cholesterol.
3. the preparation method of a kind of lotus root polyphenol-polysaccharide-liposome complex liquid according to claim 2, it is characterised in that
Step 1) described in mixed liquor the mass fraction of lipoid be 5~15%.
4. the preparation method of a kind of lotus root polyphenol-polysaccharide-liposome complex liquid according to claim 1, it is characterised in that
Step 2) in the mixed volume ratio of mixed liquor and lotus root polysaccharide solution be 3~5:1.
5. the preparation method of a kind of lotus root polyphenol-polysaccharide-liposome complex liquid according to claim 1, it is characterised in that
Step 2) in ultrasonically treated power be 300~600W, the time is 5~10min.
6. the preparation method of a kind of lotus root polyphenol-polysaccharide-liposome complex liquid according to claim 1, it is characterised in that
Step 3) in the addition of PBS be 0.5~1.5 times of volume of w/o type emulsion.
7. the preparation method of a kind of lotus root polyphenol-polysaccharide-liposome complex liquid according to any one of claim 1 to 6, its
Be characterised by, step 4) in suspension circular treatment in high pressure homogenizer refer to the circular treatment 4~8 under 100bar pressure
It is secondary.
8. a kind of lotus root polyphenol-polysaccharide-liposome complex liquid, it is characterised in that by the side described in any one of claim 1 to 7
Method is prepared.
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