CN103087213A - Preparation method of polysaccharide of tree peony seeds - Google Patents
Preparation method of polysaccharide of tree peony seeds Download PDFInfo
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- CN103087213A CN103087213A CN2013100407441A CN201310040744A CN103087213A CN 103087213 A CN103087213 A CN 103087213A CN 2013100407441 A CN2013100407441 A CN 2013100407441A CN 201310040744 A CN201310040744 A CN 201310040744A CN 103087213 A CN103087213 A CN 103087213A
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- polysaccharide
- paeonia suffruticosa
- suffruticosa seed
- tree peony
- extraction
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Abstract
The invention relates to a preparation method of polysaccharide of tree peony seeds. The method is characterized by comprising the following steps of: by taking dry powder of tree peony seeds with oil extracted as a raw material, extracting polysaccharide of tree peony seeds by using water as a solvent; performing solid-liquid separation to obtain an extracting liquid containing seed polysaccharide; adding ethanol into the extracting liquid; uniformly mixing and standing for a while at low temperature; centrifugalizing; removing supernate; collecting crude polysaccharide; removing proteins and small molecular impurities; and dehydrating to obtain the polysaccharide of tree peony seeds. The polysaccharide of tree peony seeds produced by the preparation process is shallow in color and good in water solubility, and has better oxidation resistance through test.
Description
Technical field
The present invention relates to active substance of plant and extract, specifically, be to provide a kind of preparation technology of Paeonia suffruticosa seed polysaccharide.
Technical background
Tree peony (Paeonia suffruticosa Andr.) belongs to the Ranunculaceae Paeonia, machaka, be distributed widely in Luoyang, henan, Heze, Shandong, Tongling, Anhui Province, Hanzhong, Hebei Bai Shan, Sichuan, Gansu, zhejiang and other places, according to statistics, the cultivated area of China tree peony has reached 300,000 mu, peony seeds annual production 30000 tons of left and right.
Paeonia suffruticosa seed oil was approved as new resource food in 2011 by the Ministry of Health, and after this Paeonia suffruticosa seed oil has entered a large amount of production phases, and the while has also produced the residuum after the complete grease of a large amount of extractions, so how efficiently to utilize these residuums just to become a difficult problem.Paeonia suffruticosa seed has extracted in the residuum of grease and has contained a large amount of vegetable polysaccharides compositions, and these vegetable polysaccharides compositions the experiment proved that to have the good ability such as anti-oxidant.Therefore, adopt suitable production technique that these vegetable polysaccharidess are extracted and be used to the comprehensive utilization Paeonia suffruticosa seed, improving tree peony industry added value significant.
Summary of the invention
The technical problem that the present invention solves is to provide the process program that extracts and prepare polysaccharide a kind of residuum after existing Paeonia suffruticosa seed oil extracts, this process program both can extract the polysaccharide component in Paeonia suffruticosa seed to greatest extent, can guarantee that again extracting the polysaccharide for preparing has higher anti-oxidant activity.
In order to reach above purpose, embodiment of the present invention are: take the Paeonia suffruticosa seed xeraphium that extracted grease as raw material, take water as solvent extraction Paeonia suffruticosa seed polysaccharide, the extracting solution that solid-liquid separation obtains to contain the Paeonia suffruticosa seed polysaccharide is carried out in centrifugal or filtration, add ethanol in extracting solution, make alcohol concn reach 50-90%, mix rear stand at low temperature for some time, centrifugal, remove supernatant liquor, collect Crude polysaccharides, after removing protein and small molecular weight impurity, carry out processed and obtain the Paeonia suffruticosa seed polysaccharide.
Specific embodiment 1
take the Paeonia suffruticosa seed xeraphium that extracted grease as raw material, take water as solvent, 1: 20 ultrasonic assisted extraction Paeonia suffruticosa seed polysaccharide 40min of solid-liquid ratio, extract 2 times, united extraction liquid, the centrifugal 20min of 6000r/min obtains to contain the extracting solution of Paeonia suffruticosa seed polysaccharide, add ethanol in extracting solution, make alcohol concn reach 80%, mix rear 4 ℃ of standing 3h, the centrifugal 20min of 8000r/min, remove supernatant liquor, collect Crude polysaccharides, adding 20% trichoroacetic acid(TCA) to solution final concentration in the Crude polysaccharides liquid is 5%, 4 ℃ of standing 12h, the centrifugal 5min of 3000r/min, discard precipitation, removal protein obtains supernatant liquid dialysis 48h and removes small molecular weight impurity, adopt Freeze Drying Technique to carry out processed, condenser temperature is-56 ℃, vacuum tightness is 1.0bar, obtain the Paeonia suffruticosa seed polysaccharide.
Specific embodiment 2
take the Paeonia suffruticosa seed xeraphium that extracted grease as raw material, take water as solvent, 1: 30 refluxing extraction Paeonia suffruticosa seed polysaccharide 1h of solid-liquid ratio, extract 3 times, united extraction liquid, the centrifugal 25min of 5000r/min obtains to contain the extracting solution of Paeonia suffruticosa seed polysaccharide, add ethanol in extracting solution, make alcohol concn reach 80%, mix rear 4 ℃ of standing 3h, the centrifugal 20min of 8000r/min, remove supernatant liquor, collect Crude polysaccharides, add Sevage reagent (chloroform: propyl carbinol is 4: 1) in Crude polysaccharides liquid, after fully mixing, the centrifugal 10min of 3000r/min, discard precipitation, after getting supernatant liquid, removal protein obtains supernatant liquid dialysis 48h and removes small molecular weight impurity, adopt the air seasoning method to carry out processed, temperature is 75 ℃, obtain the Paeonia suffruticosa seed polysaccharide.
Claims (8)
1. the preparation technology of a Paeonia suffruticosa seed polysaccharide, it is characterized in that: take the Paeonia suffruticosa seed xeraphium that extracted grease as raw material, take water as solvent extraction Paeonia suffruticosa seed polysaccharide, carry out the extracting solution that solid-liquid separation obtains to contain seed polysaccharide, add ethanol in extracting solution, mix rear stand at low temperature for some time, centrifugal, remove supernatant liquor, collect Crude polysaccharides, after removing protein and small molecular weight impurity, carry out processed and obtain the Paeonia suffruticosa seed polysaccharide.
2. according to Paeonia suffruticosa seed polysaccharide raw material claimed in claim 1, for adopting the residuum drying gained after cold press or hot moulding mode have been extracted tree peony oil.
3. according to the mode of water extraction Paeonia suffruticosa seed polysaccharide claimed in claim 1, it is characterized in that extracting mode is that hot water extraction method, ultrasonic assisted Extraction are followed the example of, a kind of in following the example of of microwave-assisted extraction method, enzyme assisted Extraction, raw material and water ratio (weightmeasurement ratio) they are 1: 10-1: 50, and extraction time is 1-4 time.
4. according to the solid-liquid separation method that carries out claimed in claim 1, it is characterized in that using suction filtration or centrifugation that extracting solution is separated with residue after extraction, during suction filtration, the filter aperture is not higher than 100 μ m, and when centrifugal, centrifugal speed is 1000-6000 rev/min, and centrifugation time is 5-30 minute.
5. according to claim 1ly add ethanol in extracting solution, it is characterized in that ethanol final volume concentration is 50-90%.
6. according to claim 1 centrifugal, it is characterized in that centrifugal speed is 2000-8000 rev/min, centrifugation time is 10-30 minute.
7. removal protein according to claim 1, is characterized in that using a kind of protein that removes in the Paeonia suffruticosa seed Crude polysaccharides in Sevage method, Freon 113 method, trichoroacetic acid(TCA), salt acid system, enzyme process.
8. processed according to claim 1, is characterized in that using air seasoning or Freeze Drying Technique to carry out processed to precipitation, and when using air seasoning, drying temperature is 40-80 ℃; When using lyophilize, condenser temperature is not higher than-45 ℃, and vacuum tightness is for being not less than 20bar.
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| CN2013100407441A CN103087213A (en) | 2013-02-01 | 2013-02-01 | Preparation method of polysaccharide of tree peony seeds |
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| CN2013100407441A CN103087213A (en) | 2013-02-01 | 2013-02-01 | Preparation method of polysaccharide of tree peony seeds |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103435711A (en) * | 2013-07-17 | 2013-12-11 | 新乡医学院 | Extraction method of ipomoea nil polysaccharide |
| CN103554284A (en) * | 2013-10-11 | 2014-02-05 | 青岛农业大学 | Extraction and separation process for peony stamen polysaccharide |
| CN103609751A (en) * | 2013-12-02 | 2014-03-05 | 东北林业大学 | Preparation process of water-soluble peony seed oil powder |
| CN103739732A (en) * | 2013-12-27 | 2014-04-23 | 新疆奇康哈博维药有限公司 | Method for extracting hollyhock seed polysaccharide |
| CN104031157A (en) * | 2014-06-03 | 2014-09-10 | 菏泽尧舜牡丹生物科技有限公司 | Method for extracting peony polysaccharide from peony seed meals |
| CN104292356A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Method for extracting peony polysaccharide from peony cakes with biological enzyme method |
| CN104558221A (en) * | 2013-10-25 | 2015-04-29 | 匡海学 | Preparation method of polysaccharide extracts of pharbitis seeds and processed product thereof and use of polysaccharide extracts |
| CN105294881A (en) * | 2015-12-11 | 2016-02-03 | 苏州泽达兴邦医药科技有限公司 | Method for extracting crude peony pod polysaccharide of from peony pods |
| CN106478831A (en) * | 2016-10-28 | 2017-03-08 | 郑州师范学院 | A kind of Paeonia suffruticosa polysaccharide and its preparation method and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6351401A (en) * | 1986-08-19 | 1988-03-04 | Nichiden Kagaku Kk | Production of polysaccharides |
| EP0395757A1 (en) * | 1987-09-17 | 1990-11-07 | YOSHIHARA, Masazumi | Process for extracting physiologically active substance from pine seed shells and antiinfectant prepared mainly from the extract |
| CN101443364A (en) * | 2006-05-10 | 2009-05-27 | 博胡斯生物科技股份公司 | Pectic polysaccharides isolated from fruit pods of okra |
| CN102731673A (en) * | 2012-07-05 | 2012-10-17 | 东北林业大学 | Preparation process of peony seed colloid |
-
2013
- 2013-02-01 CN CN2013100407441A patent/CN103087213A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6351401A (en) * | 1986-08-19 | 1988-03-04 | Nichiden Kagaku Kk | Production of polysaccharides |
| EP0395757A1 (en) * | 1987-09-17 | 1990-11-07 | YOSHIHARA, Masazumi | Process for extracting physiologically active substance from pine seed shells and antiinfectant prepared mainly from the extract |
| CN101443364A (en) * | 2006-05-10 | 2009-05-27 | 博胡斯生物科技股份公司 | Pectic polysaccharides isolated from fruit pods of okra |
| CN102731673A (en) * | 2012-07-05 | 2012-10-17 | 东北林业大学 | Preparation process of peony seed colloid |
Non-Patent Citations (3)
| Title |
|---|
| 单虎等主编: "《现代兽医兽药大全 动物生物制品分册》", 30 April 2011, article "《现代兽医兽药大全 动物生物制品分册》", pages: 123-125 * |
| 单虎等主编: "《现代兽医兽药大全 动物生物制品分册》", 30 April 2011, 中国农业大学出版社 * |
| 谌志伟: "《牡丹种仁多糖提取最佳工艺研究》", 《中国酿造》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103435711A (en) * | 2013-07-17 | 2013-12-11 | 新乡医学院 | Extraction method of ipomoea nil polysaccharide |
| CN103435711B (en) * | 2013-07-17 | 2016-05-18 | 新乡医学院 | A kind of extracting method of black ugly polysaccharide |
| CN103554284A (en) * | 2013-10-11 | 2014-02-05 | 青岛农业大学 | Extraction and separation process for peony stamen polysaccharide |
| CN104558221A (en) * | 2013-10-25 | 2015-04-29 | 匡海学 | Preparation method of polysaccharide extracts of pharbitis seeds and processed product thereof and use of polysaccharide extracts |
| CN103609751A (en) * | 2013-12-02 | 2014-03-05 | 东北林业大学 | Preparation process of water-soluble peony seed oil powder |
| CN103739732A (en) * | 2013-12-27 | 2014-04-23 | 新疆奇康哈博维药有限公司 | Method for extracting hollyhock seed polysaccharide |
| CN104031157A (en) * | 2014-06-03 | 2014-09-10 | 菏泽尧舜牡丹生物科技有限公司 | Method for extracting peony polysaccharide from peony seed meals |
| CN104292356A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Method for extracting peony polysaccharide from peony cakes with biological enzyme method |
| CN105294881A (en) * | 2015-12-11 | 2016-02-03 | 苏州泽达兴邦医药科技有限公司 | Method for extracting crude peony pod polysaccharide of from peony pods |
| CN106478831A (en) * | 2016-10-28 | 2017-03-08 | 郑州师范学院 | A kind of Paeonia suffruticosa polysaccharide and its preparation method and application |
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Application publication date: 20130508 |