CN104367622A - Method for extracting alkaloids from herba leonuri - Google Patents
Method for extracting alkaloids from herba leonuri Download PDFInfo
- Publication number
- CN104367622A CN104367622A CN201410632570.2A CN201410632570A CN104367622A CN 104367622 A CN104367622 A CN 104367622A CN 201410632570 A CN201410632570 A CN 201410632570A CN 104367622 A CN104367622 A CN 104367622A
- Authority
- CN
- China
- Prior art keywords
- herba leonuri
- alkaloids
- ethanol
- filter
- hydrochloric acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/533—Leonurus (motherwort)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Compounds Of Unknown Constitution (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a method for extracting alkaloids from herba leonuri. The method specifically comprises the steps of adding fresh herba leonuri into a constant-temperature furnace, drying in the presence of nitrogen, crushing and screening, carrying out ethanol soaking, ultrasonic washing and resin filtering, decompressing to recycle solvents, drying the solvents into paste by distillation, carrying out water-bath heating by virtue of hydrochloric acid and active carbon, carrying out elution, and finally carrying out compression concentration on an eluted solution, so as to obtain the alkaloids in herba leonuri.
Description
Technical field
The present invention relates to a kind of extracting method, particularly the extracting method of alkaloids in a kind of Herba Leonuri.
Background technology
Herba Leonuri is one of conventional Chinese medicine, records in history tree detailed outline, belongs to Labiatae Herba Leonuri and belongs to various plants together, and the most frequently used be Herba Leonuri.Be used as medicine with the dry product of the aerial parts of bud stage or fresh goods, have the effect such as promoting blood flow to regulate menstruation, inducing diuresis to remove edema.Clinical menoxenia, vaginal bleeding during pregnancy difficult labour, dysmenorrhea and the postnatal blood stasis of being used for is suffered from abdominal pain, and have the title of " blood man panacea ", " multiparity good medicine ".Domestic and international numerous scholars have carried out long-term research to Herba Leonuri, obtain a lot of achievement, in Herba Leonuri, main chemical compositions contains alkaloids, flavonoid, organic acid, various trace elements and fatty acid etc., wherein studying more is alkaloids, and this is also have the bioactive element of the first species.Clinical conventional be Herba Leonuri raw medicinal herbs and motherwort formulation, its quality control take total alkaloids as index.
The extraction process of the alkaloids in Herba Leonuri all adopts the method for water extract-alcohol precipitation, concentrate drying always, and the effective component yield of the method is low, impure more, constrains and it is made modern dosage form further.
Summary of the invention
The present invention is directed to that traditional effective component yield is low, impurity content is high and affect the problems such as product quality, propose alkaloids extracting method in a kind of Herba Leonuri, the method effective component yield is high, without problems such as qualitative effects.
In order to reach above-mentioned purpose, the concrete technical scheme of employing is:
(1) fresh Herba Leonuri is put in constant temperature oven, under the protection of nitrogen at 50-55 DEG C dry 1-2h;
(2) dried Herba Leonuri is put in pulverizer pulverize, through sieving out 40-50 object motherwort powder;
(3) accurately take 3g motherwort powder and be placed in conical flask, add the ethanol that 20mL mass fraction is 95%, soak 1-2h, soak rear ultrasonic washing 2-3 time, then resin filter is used, filtrate decompression recycling design, then put it into transpiring moisture in Rotary Evaporators, until institute's extract matter is paste;
(4) add to it ethanol that 30mL mass fraction is 80%, supersound process 30min, lets cool, and in its weight of title, supply the weight of less loss, shake up with ethanol, filter, precision measures subsequent filtrate 25mL, heats to evaporating dish in water-bath;
(5) the hydrochloric acid solution 10mL that precision adds 0.15mol/L dissolves, and adds active carbon 0.5g, puts in water-bath and heat half a minute, agitation and filtration, in the 25mL volumetric flask of greenery, divide with the hydrochloric acid solution of 0.15mol/L and wash evaporating dish and filter 2 times, washing liquid is incorporated in same measuring bottle;
(6) carry out concentrating under reduced pressure at finally eluent being put in 70 DEG C, namely obtain the alkaloids in Herba Leonuri, alkaloids is placed in spectrophotometer and detects.
Specific embodiments
(1) fresh Herba Leonuri is put in constant temperature oven, under the protection of nitrogen at 50-55 DEG C dry 1-2h;
(2) dried Herba Leonuri is put in pulverizer pulverize, through sieving out 40-50 object motherwort powder;
(3) accurately take 3g motherwort powder and be placed in conical flask, add the ethanol that 20mL mass fraction is 95%, soak 1-2h, soak rear ultrasonic washing 2-3 time, then resin filter is used, filtrate decompression recycling design, then put it into transpiring moisture in Rotary Evaporators, until institute's extract matter is paste;
(4) add to it ethanol that 30mL mass fraction is 80%, supersound process 30min, lets cool, and in its weight of title, supply the weight of less loss, shake up with ethanol, filter, precision measures subsequent filtrate 25mL, heats to evaporating dish in water-bath;
(5) the hydrochloric acid solution 10mL that precision adds 0.15mol/L dissolves, and adds active carbon 0.5g, puts in water-bath and heat half a minute, agitation and filtration, in the 25mL volumetric flask of greenery, divide with the hydrochloric acid solution of 0.15mol/L and wash evaporating dish and filter 2 times, washing liquid is incorporated in same measuring bottle;
(6) carry out concentrating under reduced pressure at finally eluent being put in 70 DEG C, namely obtain the alkaloids in Herba Leonuri, alkaloids is placed in spectrophotometer and detects.
Example 1
Fresh Herba Leonuri is put in constant temperature oven, under the protection of nitrogen at 50 DEG C dry 1h; Dried Herba Leonuri is put in pulverizer and pulverizes, through sieving out 40 object motherwort powders; Accurately take 3g motherwort powder and be placed in conical flask, add the ethanol that 20mL mass fraction is 95%, soak 1h, soak rear ultrasonic washing 2 times, then used resin filter, filtrate decompression recycling design, put it into transpiring moisture in Rotary Evaporators again, until institute's extract matter is paste; Add to it ethanol that 30mL mass fraction is 80%, supersound process 30min, lets cool, and in its weight of title, supply the weight of less loss, shake up with ethanol, filter, precision measures subsequent filtrate 25mL, heats to evaporating dish in water-bath; The hydrochloric acid solution 10mL that precision adds 0.15mol/L dissolves, and adds active carbon 0.5g, puts in water-bath and heat half a minute, agitation and filtration, in the 25mL volumetric flask of greenery, divide with the hydrochloric acid solution of 0.15mol/L and wash evaporating dish and filter 2 times, washing liquid is incorporated in same measuring bottle; Carry out concentrating under reduced pressure at finally eluent being put in 70 DEG C, namely obtain the alkaloids in Herba Leonuri.
Example 2
Fresh Herba Leonuri is put in constant temperature oven, under the protection of nitrogen at 53 DEG C dry 1.5h; Dried Herba Leonuri is put in pulverizer and pulverizes, through sieving out 45 object motherwort powders; Accurately take 3g motherwort powder and be placed in conical flask, add the ethanol that 20mL mass fraction is 95%, soak 1.5h, soak rear ultrasonic washing 2 times, then used resin filter, filtrate decompression recycling design, put it into transpiring moisture in Rotary Evaporators again, until institute's extract matter is paste; Add to it ethanol that 30mL mass fraction is 80%, supersound process 30min, lets cool, and in its weight of title, supply the weight of less loss, shake up with ethanol, filter, precision measures subsequent filtrate 25mL, heats to evaporating dish in water-bath; The hydrochloric acid solution 10mL that precision adds 0.15mol/L dissolves, and adds active carbon 0.5g, puts in water-bath and heat half a minute, agitation and filtration, in the 25mL volumetric flask of greenery, divide with the hydrochloric acid solution of 0.15mol/L and wash evaporating dish and filter 2 times, washing liquid is incorporated in same measuring bottle; Carry out concentrating under reduced pressure at finally eluent being put in 70 DEG C, namely obtain the alkaloids in Herba Leonuri.
Example 3
Fresh Herba Leonuri is put in constant temperature oven, under the protection of nitrogen at 55 DEG C dry 2h; Dried Herba Leonuri is put in pulverizer and pulverizes, through sieving out 50 object motherwort powders; Accurately take 3g motherwort powder and be placed in conical flask, add the ethanol that 20mL mass fraction is 95%, soak 2h, soak rear ultrasonic washing 3 times, then used resin filter, filtrate decompression recycling design, put it into transpiring moisture in Rotary Evaporators again, until institute's extract matter is paste; Add to it ethanol that 30mL mass fraction is 80%, supersound process 30min, lets cool, and in its weight of title, supply the weight of less loss, shake up with ethanol, filter, precision measures subsequent filtrate 25mL, heats to evaporating dish in water-bath; The hydrochloric acid solution 10mL that precision adds 0.15mol/L dissolves, and adds active carbon 0.5g, puts in water-bath and heat half a minute, agitation and filtration, in the 25mL volumetric flask of greenery, divide with the hydrochloric acid solution of 0.15mol/L and wash evaporating dish and filter 2 times, washing liquid is incorporated in same measuring bottle; Carry out concentrating under reduced pressure at finally eluent being put in 70 DEG C, namely obtain the alkaloids in Herba Leonuri.
Claims (1)
1. the extracting method of alkaloids in Herba Leonuri, is characterized in that:
(1) fresh Herba Leonuri is put in constant temperature oven, under the protection of nitrogen at 50-55 DEG C dry 1-2h;
(2) dried Herba Leonuri is put in pulverizer pulverize, through sieving out 40-50 object motherwort powder;
(3) accurately take 3g motherwort powder and be placed in conical flask, add the ethanol that 20mL mass fraction is 95%, soak 1-2h, soak rear ultrasonic washing 2-3 time, then resin filter is used, filtrate decompression recycling design, then put it into transpiring moisture in Rotary Evaporators, until institute's extract matter is paste;
(4) add to it ethanol that 30mL mass fraction is 80%, supersound process 30min, lets cool, and in its weight of title, supply the weight of less loss, shake up with ethanol, filter, precision measures subsequent filtrate 25mL, heats to evaporating dish in water-bath;
(5) the hydrochloric acid solution 10mL that precision adds 0.15mol/L dissolves, and adds active carbon 0.5g, puts in water-bath and heat half a minute, agitation and filtration, in the 25mL volumetric flask of greenery, divide with the hydrochloric acid solution of 0.15mol/L and wash evaporating dish and filter 2 times, washing liquid is incorporated in same measuring bottle;
(6) carry out concentrating under reduced pressure at finally eluent being put in 70 DEG C, namely obtain the alkaloids in Herba Leonuri, alkaloids is placed in spectrophotometer and detects.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410632570.2A CN104367622A (en) | 2014-11-11 | 2014-11-11 | Method for extracting alkaloids from herba leonuri |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410632570.2A CN104367622A (en) | 2014-11-11 | 2014-11-11 | Method for extracting alkaloids from herba leonuri |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104367622A true CN104367622A (en) | 2015-02-25 |
Family
ID=52546983
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410632570.2A Pending CN104367622A (en) | 2014-11-11 | 2014-11-11 | Method for extracting alkaloids from herba leonuri |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104367622A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105434278A (en) * | 2015-12-30 | 2016-03-30 | 云南人羞花化妆品有限公司 | Panax notoginseng toner and preparation method thereof |
| CN106729588A (en) * | 2017-03-03 | 2017-05-31 | 四川德成动物保健品有限公司 | Motherwort extraction process of effective component based on beneficial female biochemistry mixture |
| CN109180534A (en) * | 2018-09-10 | 2019-01-11 | 合肥科佳高分子材料科技有限公司 | A kind of method of Total alkaloids eluent desalination |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1467204A (en) * | 2002-07-10 | 2004-01-14 | 江苏康缘药业股份有限公司 | Method for preparing motherwort total alkaloid and new medicine uses |
| CN101244121A (en) * | 2007-02-15 | 2008-08-20 | 北京天新园医药科技开发有限公司 | Leonurus heterophyllus total alkaloid extract and preparation thereof |
-
2014
- 2014-11-11 CN CN201410632570.2A patent/CN104367622A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1467204A (en) * | 2002-07-10 | 2004-01-14 | 江苏康缘药业股份有限公司 | Method for preparing motherwort total alkaloid and new medicine uses |
| CN101244121A (en) * | 2007-02-15 | 2008-08-20 | 北京天新园医药科技开发有限公司 | Leonurus heterophyllus total alkaloid extract and preparation thereof |
Non-Patent Citations (2)
| Title |
|---|
| 付君叶: "利用超声法从益母草中提取总生物碱的研究", 《江西理工大学学报》 * |
| 周本宏: "超声提取益母草总碱的工艺优选", 《医药导报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105434278A (en) * | 2015-12-30 | 2016-03-30 | 云南人羞花化妆品有限公司 | Panax notoginseng toner and preparation method thereof |
| CN106729588A (en) * | 2017-03-03 | 2017-05-31 | 四川德成动物保健品有限公司 | Motherwort extraction process of effective component based on beneficial female biochemistry mixture |
| CN109180534A (en) * | 2018-09-10 | 2019-01-11 | 合肥科佳高分子材料科技有限公司 | A kind of method of Total alkaloids eluent desalination |
| CN109180534B (en) * | 2018-09-10 | 2021-04-23 | 合肥科佳高分子材料科技有限公司 | Method for desalting motherwort total alkali eluent |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104710391A (en) | Method for extracting luteolin and beta-sitosterol from peanut shells | |
| CN103087213A (en) | Preparation method of polysaccharide of tree peony seeds | |
| CN104523767A (en) | Method for extracting flavones substances in cacumen biotae | |
| CN104629902A (en) | Method for extracting maize germ oil assisted by ethyl alcohol and steam explosion | |
| CN103432562A (en) | Method for extracting fresh ginger polyphenol from fresh ginger | |
| CN101530445A (en) | Dry extract of chrysanthemum | |
| CN102206550B (en) | Method for preparing osmanthus concrete and osmanthus total flavonoid through enzyme hydrolysis technology | |
| CN104367622A (en) | Method for extracting alkaloids from herba leonuri | |
| CN102146143B (en) | Method for preparing pectin and cellulose from extracting residues of ginseng and American ginseng and application thereof | |
| CN103923050B (en) | Utilize rutin to prepare the method for Quercetin | |
| Rohmah et al. | Optimization of Extraction of Bioactive Compound from Pegagan Leaves Using Ethanol Solvent With Microwave-Assisted Extraction Method (MAE) | |
| CN104356105B (en) | A kind of preparation method of EGCG | |
| CN101584752B (en) | Extraction and purification process for total flavonoids in Anchusa italica Retiz | |
| CN104098636B (en) | A kind of method extracting rutin in the Flos Sophorae Immaturus | |
| CN108126000A (en) | Arasaponin extracts preparation method in fresh Radix Notoginseng | |
| CN106187978A (en) | A kind of method extracting pitaya peel procyanidins | |
| CN108276467A (en) | A kind of Tea Saponin and its extraction process and application | |
| CN102846719B (en) | Method for processing of Green Fructus forsythiae in producing area | |
| CN104074086A (en) | Method for extracting cellulose from sow thistle | |
| CN104177462B (en) | A kind of method of Hydrolysis kinetics rutin from yulan petal | |
| CN102241656A (en) | Preparation method of tricin | |
| CN105085705A (en) | Method for extracting inulin from burdock root | |
| CN103948653A (en) | Process for extracting broadleaf holly leaf tea polyphenol from broadleaf holly leaf | |
| CN112043738A (en) | Preparation method of radix angelicae pubescentis active ingredient extract | |
| CN105708882A (en) | Extraction process of sweet potato fol. flavone |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150225 |