CN105085705A - Method for extracting inulin from burdock root - Google Patents

Abstract

The invention discloses a method for extracting inulin from burdock root. The method for extracting inulin from the burdock root comprises the following steps: cleaning the burdock root, drying the burdock root in the sun, and smashing to obtain burdock root powder for later use; adding hot water at the temperature of 80 DEG C, fully stirring uniformly, and carrying out heat preservation and soaking extraction twice, mixing filtrate obtained twice, and filtering while the filtrate is hot, and collecting the filtrate; heating, evaporating and concentrating the filtrate, adding 95-degree ethyl alcohol, fully stirring and standing for 2 hours, and filtering for removing the filtrate, so that precipitate, namely an inulin crude product, is obtained; adding ten times weight of distilled water into the inulin crude product, stirring until the inulin crude product is thoroughly dissolved, and filtering for removing filter residues; adding 95-degree ethyl alcohol into the filtrate, wherein the volume ratio of the filtrate to ethyl alcohol is 1:2; uniformly stirring, and standing for 12 hours at the temperature of 0-4 DEG C; and filtering for obtaining filter residues, and drying, so that the inulin finished product is obtained. The method for extracting inulin from the burdock root mainly comprises the steps of smashing, soaking, concentrating, precipitating, purifying and drying, inulin can be rapidly and simply extracted, the economical efficiency is good, and the extraction efficiency is high.

Description

A kind of method extracting synanthrin from Root of Great Burdock

technical fieldthe invention belongs to synanthrin extractive technique field, be specifically related to a kind of method extracting synanthrin from Root of Great Burdock.

background technologyburdock, has another name called and dislikes real, Fructus Arctii, be also Japan's pizzle dish etc., and burdock has treats diabetes, hypertension, hyperlipidemia, the effect such as anticancer; Can hypoglycemic, hypotensive, blood fat, Cure for insomnia, improve the effects such as body immunity.Root of Great Burdock is also containing synanthrin and volatile oil, burdock shortcake, and rich cellulose and amino acid; Synanthrin is medically mainly used in the filterability measuring renal glomerulus, owing to not containing synanthrin in human body, after quiet note, do not decomposed by body, combine, utilize and destroy, be unique excretion pathway through glomerular filtration, uriniferous tubules is neither secreted and is not also heavily absorbed, by measuring the inulin concentration in blood and in urine, carry out the filterability of accurate calculation renal glomerulus, namely inulin clearance equals glomerular filtration rate(GFR; The known plant containing synanthrin has kind more than 30,000 at present, comparatively tradition in extraction process and method, low, the consuming time length of extraction yield and power consumption is large; Therefore, a kind of not only easy but also economic method can extracting synanthrin fast from Root of Great Burdock is needed.

summary of the inventionthe object of this invention is to provide a kind of economy, fast and the high method extracting synanthrin from Root of Great Burdock of extraction yield, to make up the deficiencies in the prior art.

To achieve these goals, a kind of method extracting synanthrin from Root of Great Burdock of the present invention, carry out in the steps below:

1) by after clean for Root of Great Burdock drying, pulverize burdock root powder is for subsequent use;

2) add 900 ~ 1100ml temperature be the hot water of 80 DEG C and stir, water temperature leaches filtrate after Soakage extraction 2-3 hour, Soakage extraction twice repeatedly under keeping the prerequisite of 75 ~ 85 DEG C, merges the filtrate filtered while hot of extracted twice, collects filtrate;

3), when filtrate heating evaporation being concentrated into 200 ~ 350ml, Temperature fall to 20 is DEG C for subsequent use;

4) add 400 ~ 600ml95 degree ethanol, fully stir 5 ~ 7min and leave standstill after 2 ~ 3h, filtering filtrate obtains throw out, is synanthrin crude product;

5) distilled water that synanthrin crude product step 4) obtained adds 9 ~ 11 times amount stirs 20 ~ 30min to thoroughly dissolving, filtering filter residue;

6) in the filtrate of step 5), add 95 degree of ethanol, the volume ratio of filtrate and ethanol is 1:2, stirs and 11 ~ 13h under being placed in the environment of 0 ~ 4 DEG C;

7) filter to obtain filter residue, at the temperature of 75 ~ 85 DEG C, namely dry 15-25min obtains synanthrin finished product.

The present invention is a kind of extracts the method for synanthrin from Root of Great Burdock, main through pulverizing, dipping, concentrated, precipitation, purify, dry step, extraction synanthrin that can be quick, easy, good economy performance, extraction yield are high.

Embodiment

Embodiment one

Root of Great Burdock is cleaned after drying, pulverize burdock root powder is for subsequent use; Add 1000ml temperature be the hot water of 80 DEG C and stir, under water temperature keeps the prerequisite of 80 DEG C, Soakage extraction leached filtrate after 2 hours, Soakage extraction twice repeatedly, merged the filtrate filtered while hot of extracted twice, collected filtrate; When filtrate heating evaporation is concentrated into 200ml, Temperature fall to 20 is DEG C for subsequent use; Add 400ml95 degree ethanol, after fully stirring 5min also leaves standstill 2h, filtering filtrate obtains throw out, is synanthrin crude product; The distilled water obtained synanthrin crude product being added 10 times amount stirs 20min to thoroughly dissolving, filtering filter residue; In filtrate, add 95 degree of ethanol, the volume ratio of filtrate and ethanol is 1:2, stirs and 12h under being placed in the environment of 0 ~ 4 DEG C; Filter to obtain filter residue, at the temperature of 85 DEG C, namely dry 15min obtains synanthrin finished product.

Embodiment two

Root of Great Burdock is cleaned after drying, pulverize burdock root powder is for subsequent use; Add 1100ml temperature be the hot water of 85 DEG C and stir, under water temperature keeps the prerequisite of 85 DEG C, Soakage extraction leached filtrate after 3 hours, Soakage extraction twice repeatedly, merged the filtrate filtered while hot of extracted twice, collected filtrate; When filtrate heating evaporation is concentrated into 350ml, Temperature fall to 20 is DEG C for subsequent use; Add 500ml95 degree ethanol, after fully stirring 7min also leaves standstill 3h, filtering filtrate obtains throw out, is synanthrin crude product; The distilled water obtained synanthrin crude product being added 11 times amount stirs 30min to thoroughly dissolving, filtering filter residue; In filtrate, add 95 degree of ethanol, the volume ratio of filtrate and ethanol is 1:2, stirs and 13h under being placed in the environment of 0 ~ 4 DEG C; Filter to obtain filter residue, at the temperature of 75 DEG C, namely dry 25min obtains synanthrin finished product.

Embodiment three

Root of Great Burdock is cleaned after drying, pulverize burdock root powder is for subsequent use; Add 900ml temperature be the hot water of 75 DEG C and stir, under water temperature keeps the prerequisite of 85 DEG C, Soakage extraction leached filtrate after 2.5 hours, Soakage extraction twice repeatedly, merged the filtrate filtered while hot of extracted twice, collected filtrate; When filtrate heating evaporation is concentrated into 300ml, Temperature fall to 20 is DEG C for subsequent use; Add 600ml95 degree ethanol, after fully stirring 6min also leaves standstill 3h, filtering filtrate obtains throw out, is synanthrin crude product; The distilled water obtained synanthrin crude product being added 9 times amount stirs 25min to thoroughly dissolving, filtering filter residue; In filtrate, add 95 degree of ethanol, the volume ratio of filtrate and ethanol is 1:2, stirs and 11h under being placed in the environment of 0 ~ 4 DEG C; Filter to obtain filter residue, at the temperature of 80 DEG C, namely dry 20min obtains synanthrin finished product.

All distortion that those of ordinary skill in the art can not make creative work and directly derive or associate from content disclosed by the invention, all should drop within protection scope of the present invention.

Claims (1)

1. extract the method for synanthrin from Root of Great Burdock, it is characterized in that realizing as follows:

1) by after clean for Root of Great Burdock drying, pulverize burdock root powder is for subsequent use;

2) add 900 ~ 1100ml temperature be the hot water of 80 DEG C and stir, water temperature leaches filtrate after Soakage extraction 2-3 hour, Soakage extraction twice repeatedly under keeping the prerequisite of 75 ~ 85 DEG C, merges the filtrate filtered while hot of extracted twice, collects filtrate;

3), when filtrate heating evaporation being concentrated into 200 ~ 350ml, Temperature fall to 20 is DEG C for subsequent use;

4) add 400 ~ 600ml95 degree ethanol, fully stir 5 ~ 7min and leave standstill after 2 ~ 3h, filtering filtrate obtains throw out, is synanthrin crude product;

5) distilled water that synanthrin crude product step 4) obtained adds 9 ~ 11 times amount stirs 20 ~ 30min to thoroughly dissolving, filtering filter residue;

6) in the filtrate of step 5), add 95 degree of ethanol, the volume ratio of filtrate and ethanol is 1:2, stirs and 11 ~ 13h under being placed in the environment of 0 ~ 4 DEG C;

7) filter to obtain filter residue, at the temperature of 75 ~ 85 DEG C, namely dry 15-25min obtains synanthrin finished product.