CN103467617A - Method for continuous counter-current ultrasonic extraction of high-purity astragalus polysaccharide - Google Patents
Method for continuous counter-current ultrasonic extraction of high-purity astragalus polysaccharide Download PDFInfo
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- CN103467617A CN103467617A CN 201310425497 CN201310425497A CN103467617A CN 103467617 A CN103467617 A CN 103467617A CN 201310425497 CN201310425497 CN 201310425497 CN 201310425497 A CN201310425497 A CN 201310425497A CN 103467617 A CN103467617 A CN 103467617A
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Abstract
The invention discloses a method for continuous counter-current ultrasonic extraction of high-purity astragalus polysaccharide. The method comprises the following processing steps: crushing astragalus membranaceus and soaking into distilled water; centrifuging and taking supernate after continuous counter-current ultrasonic extraction; decoloring by active carbon, so as to obtain an astragalus polysaccharide water extract solution; then concentrating and adding ethanol to stir; centrifuging and diluting sediments by the distilled water; carrying out alcohol precipitation after filtering diluents by a membrane, wherein the sediment is astragalus polysaccharide solid. By adopting the method, the dissolution rate of the astragalus polysaccharide can be improved; the extraction time is shortened; the dosage of a solvent is reduced. Therefore, the method has the characteristics of high selectivity, simple process, low energy consumption, high yield, high product purity and the like.
Description
Technical field
The invention belongs to the vegetable chemistry technical field, be specifically related to a kind of method of continuous countercurrent supersound extraction high purity astragalus polysaccharides.
Background technology
The Radix Astragali is the dry root of leguminous plants Radix Astagali or Radix Astragali.Distinguish the flavor of sweet, slightly warm in nature, have the effect of invigorating QI to consolidate the body surface resistance, diuresis toxin expelling, apocenosis, expelling pus and promoting granulation,, anorexia and loose stool weak for the deficiency of vital energy, sinking of QI of middle-JIAO, rushes down the diseases such as prolapse of the anus for a long time.Radix Astragali pharmaceutical use is higher, modern pharmacology and Chemistry for Chinese Traditional Medicine research show: contain astragalus polysaccharides in the Radix Astragali, the composition such as Cyclosiversioside F and multiple amino acids, wherein astragalus polysaccharides is maximum a kind of of content, its main biological activity shows as the enhancing immunologic function, anti influenza, antitumor, anti-aging and radioprotective, the anxious Terrier heart is improved to shrinkage, dwindle infarct size, alleviate the effects such as myocardial damage, due to the good clinical effectiveness of the multiple biologically active agent of astragalus polysaccharides, become for many years one of focus of traditional Chinese medicine extraction and herbal medicine Study on Modernization at home and abroad.
Recent two decades comes, report the method for multiple extraction polysaccharide, mainly comprised water boiling and precipitation with ethanol method, microwave extraction method, ultrasonic extraction, ultrafiltration process etc., generally taked the water boiling and precipitation with ethanol method, because of this method technique simple operations convenience, but general water boiling and precipitation with ethanol method poach temperature is all carried out at 100 ℃.The water boiling and precipitation with ethanol method, also be extracted because excess Temperature makes flavones, saponins etc., in subsequent technique, is difficult for again they are separated, and causes the not pure and mild wasting of resources of product.Microwave method is higher for equipment requirements, implements difficulty, is difficult to large-scale application.Although acid or alkali method can improve extraction yield, the glycosidic bond of sour destructible polysaccharide, form more monose and oligose, reduces astragalus polysaccharides content.Alkaline extraction also can damage polysaccharide structures.In addition, after acid, alkali lixiviate, need through neutralization, subsequent disposal program complexity.
Summary of the invention
The purpose of this invention is to provide a kind of method of extracting the high purity astragalus polysaccharides, the method has that technique is simple, yield is large, purity is high, polysaccharide can be not destroyed, and environment amenable advantage, be easy to realize suitability for industrialized production.
The present invention includes following steps:
1. the Radix Astragali is pulverized, be dipped in distilled water by the mass volume ratio of 1:5 ~ 1:10, then under 50 ~ 70 ℃, 40 ~ 80KHz after continuous countercurrent supersound extraction 1 ~ 2h, centrifugal, get supernatant liquor, with activated carbon decolorizing, obtain astragalus polysaccharides aqueous extract solution;
2. be concentrated into to solid content at 30 ~ 40% astragalus polysaccharides concentrated solution, add 80 ~ 90% ethanolic soln of 2 ~ 4 times of amounts to stir 20 ~ 30min, centrifugal, the distilled water diluting of 3 ~ 5 times for precipitation;
3. diluent ultrafiltration membrance filter, the molecular weight cut-off of ultra-filtration membrane is 1000 ~ 10000 dalton, the filtrate alcohol precipitation obtains highly purified astragalus polysaccharides solid.
Centrifugal speed described in the present invention is 3000r/min, and centrifugation time is 10min.
Compared with prior art, the present invention has the following advantages:
(1) the present invention combines countercurrent leaching technology and Ultrasonic Wave-Assisted Extraction technology, for extracting the process of astragalus polysaccharides, accelerated the solubility rate of effective constituent in the Radix Astragali, shortened extraction time, reduced the usage quantity of solvent, simple to operate, extraction yield is high, can significantly reduce costs, realize suitability for industrialized production.
(2) adopt the ultra-filtration technique purifying, do not need heating in process, avoid the polysaccharide decomposes.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiment.
embodiment:
Embodiment 1:
The Radix Astragali is pulverized, be dipped in distilled water by the mass volume ratio of 1:5, then under 50 ℃, 40KHz after continuous countercurrent supersound extraction 1h, centrifugal, get supernatant liquor, with activated carbon decolorizing, obtain astragalus polysaccharides aqueous extract solution; Be concentrated into to solid content at 30% astragalus polysaccharides concentrated solution, add 80% ethanolic soln of 2 times of amounts to stir 25min; Centrifugal, precipitate the distilled water diluting with 5 times; The diluent ultrafiltration membrance filter, the molecular weight cut-off of ultra-filtration membrane is 5000 dalton, and the filtrate alcohol precipitation obtains highly purified astragalus polysaccharides solid, and purity is 88.6%.
Embodiment 2:
The Radix Astragali is pulverized, be dipped in distilled water by the mass volume ratio of 1:8, then under 65 ℃, 80KHz after continuous countercurrent supersound extraction 1.5h, centrifugal, get supernatant liquor, with activated carbon decolorizing, obtain astragalus polysaccharides aqueous extract solution; Be concentrated into to solid content at 35% astragalus polysaccharides concentrated solution, add 85% ethanolic soln of 3 times of amounts to stir 30min; Centrifugal, precipitate the distilled water diluting with 4 times; The diluent ultrafiltration membrance filter, the molecular weight cut-off of ultra-filtration membrane is 8000 dalton, and the filtrate alcohol precipitation obtains highly purified astragalus polysaccharides solid, and purity is 94.3%.
Embodiment 3
: the Radix Astragali is pulverized, be dipped in distilled water by the mass volume ratio of 1:10, then under 70 ℃, 60KHz after continuous countercurrent supersound extraction 2h, centrifugal, get supernatant liquor, with activated carbon decolorizing, obtain astragalus polysaccharides aqueous extract solution; Be concentrated into to solid content at 40% astragalus polysaccharides concentrated solution, add 90% ethanolic soln of 4 times of amounts to stir 30min; Centrifugal, precipitate the distilled water diluting with 3 times; The diluent ultrafiltration membrance filter, the molecular weight cut-off of ultra-filtration membrane is 3000 dalton, and the filtrate alcohol precipitation obtains highly purified astragalus polysaccharides solid, and purity is 91.8%.
Claims (3)
1. the method for a continuous countercurrent supersound extraction high purity astragalus polysaccharides, is characterized in that, comprises the following steps:
A. the Radix Astragali is pulverized, be dipped in distilled water by the mass volume ratio of 1:5 ~ 1:10, then under 50 ~ 70 ℃, 40 ~ 80KHz after continuous countercurrent supersound extraction 1 ~ 2h, centrifugal, get supernatant liquor, with activated carbon decolorizing, obtain astragalus polysaccharides aqueous extract solution;
B. be concentrated into to solid content at 30 ~ 40% astragalus polysaccharides concentrated solution, add 80 ~ 90% ethanolic soln of 2 ~ 4 times of amounts to stir 20 ~ 30min, centrifugal, the distilled water diluting of 3 ~ 5 times for precipitation;
C. diluent ultrafiltration membrance filter, the filtrate alcohol precipitation, obtain highly purified astragalus polysaccharides solid.
2. the method for a kind of continuous countercurrent supersound extraction high purity astragalus polysaccharides according to claim 1, it is characterized in that: the centrifugal speed described in step a and b is 3000r/min, centrifugation time is 10min.
3. the method for a kind of continuous countercurrent supersound extraction high purity astragalus polysaccharides according to claim 1, it is characterized in that: in step c, the molecular weight cut-off of selected ultra-filtration membrane is 1000 ~ 10000 dalton.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104558224A (en) * | 2014-12-08 | 2015-04-29 | 向华 | Method for preparing astragalus polysaccharide and water reservoir gel from astragalus residues |
| CN104974851A (en) * | 2015-07-06 | 2015-10-14 | 湖北中烟工业有限责任公司 | Preparation method for black plum extract for cigarette |
| CN105504078A (en) * | 2015-12-14 | 2016-04-20 | 陈燕妮 | Process for producing astragalus polysaccharide by using countercurrent water extraction technology |
| CN106749743A (en) * | 2017-01-23 | 2017-05-31 | 同舟纵横（厦门）流体技术有限公司 | A kind of celestial grass Polyose extraction system and extraction process |
| CN106892996A (en) * | 2017-04-01 | 2017-06-27 | 林婷 | One kind lifting immunity polysaccharide extract and its manufacture method |
| CN109134698A (en) * | 2018-08-29 | 2019-01-04 | 新疆正生营养研究院（有限公司） | A kind of witloof dregs of rice pectin preparation method |
| CN110183545A (en) * | 2019-06-14 | 2019-08-30 | 台州学院 | A kind of extraction of blue or green money willow polysaccharide and discoloration method |
| CN111925457A (en) * | 2020-07-24 | 2020-11-13 | 哈工大机器人南昌智能制造研究院 | Method for preparing high-purity lycium ruthenicum polysaccharide |
| CN112353809A (en) * | 2020-11-23 | 2021-02-12 | 上海市第五人民医院 | Pharmaceutical application of astragaloside IV compound |
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2013
- 2013-09-18 CN CN 201310425497 patent/CN103467617A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104558224A (en) * | 2014-12-08 | 2015-04-29 | 向华 | Method for preparing astragalus polysaccharide and water reservoir gel from astragalus residues |
| CN104974851A (en) * | 2015-07-06 | 2015-10-14 | 湖北中烟工业有限责任公司 | Preparation method for black plum extract for cigarette |
| CN104974851B (en) * | 2015-07-06 | 2018-06-08 | 湖北中烟工业有限责任公司 | A kind of preparation method of black cloth woods cigarette extract |
| CN105504078A (en) * | 2015-12-14 | 2016-04-20 | 陈燕妮 | Process for producing astragalus polysaccharide by using countercurrent water extraction technology |
| CN106749743A (en) * | 2017-01-23 | 2017-05-31 | 同舟纵横（厦门）流体技术有限公司 | A kind of celestial grass Polyose extraction system and extraction process |
| CN106892996A (en) * | 2017-04-01 | 2017-06-27 | 林婷 | One kind lifting immunity polysaccharide extract and its manufacture method |
| CN109134698A (en) * | 2018-08-29 | 2019-01-04 | 新疆正生营养研究院（有限公司） | A kind of witloof dregs of rice pectin preparation method |
| CN110183545A (en) * | 2019-06-14 | 2019-08-30 | 台州学院 | A kind of extraction of blue or green money willow polysaccharide and discoloration method |
| CN110183545B (en) * | 2019-06-14 | 2021-06-11 | 台州学院 | Extraction and decoloration method of cyclocarya paliurus polysaccharide |
| CN111925457A (en) * | 2020-07-24 | 2020-11-13 | 哈工大机器人南昌智能制造研究院 | Method for preparing high-purity lycium ruthenicum polysaccharide |
| CN112353809A (en) * | 2020-11-23 | 2021-02-12 | 上海市第五人民医院 | Pharmaceutical application of astragaloside IV compound |
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