CN106892996A - One kind lifting immunity polysaccharide extract and its manufacture method - Google Patents
One kind lifting immunity polysaccharide extract and its manufacture method Download PDFInfo
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Abstract
The invention discloses one kind lifting immunity polysaccharide extract and its manufacture method, the lifting immunity polysaccharide extract is made up of the composition of following weight portion：40 50 parts of Shitake Mushroom P.E, 12 15 parts of Coriolus Versicolor P.E., 10 15 parts of Fructus lycii P.E, 8 10 parts of Astragalus Root P.E；The extraction of its manufacture method including Shitake Mushroom P.E, the extraction of Coriolus Versicolor P.E., the extraction of Fructus lycii P.E, the extraction of Astragalus Root P.E, desired polysaccharide class extract such as produce at five steps.It is of the invention lifting immunity polysaccharide extract and its manufacture method it is applied widely, effective ingredient is more, effective ingredient has complementary functions mutually promote, extraction efficiency it is high, acquisition product purity it is high, be easily achieved.
Description
Technical field
The present invention relates to biomedicine field, more particularly to a kind of immunity that lifted is with polysaccharide extract and its manufacturer
Method.
Background technology
Plant polyose, is the glycan of the degree of polymerization more than 10 of plant metabolism generation also known as plant polysaccharide.Be by
The compound that many identical or different monose are constituted with α mono- or the glycosidic bonds of β mono-, is prevalent in natural plant body,
Including starch, cellulose, polysaccharide, pectin etc..Due to the wide material sources of plant polyose, the molecule structure of plant polyose not of the same race
Into and molecular weight it is different.Some plant polyoses such as starch, cellulose, pectin, already as the weight in people's daily life
Want part.
Plant polyose research now is of increasing concern, and international scientific circle even proposes that 21 century is the century of polysaccharide；Section
Learn experimental study and show that many plant polyoses have bioactivity, with including immunological regulation, antitumor, hypoglycemic, drop blood
The health-care effects such as fat, radioresistance, anti-bacteria and anti-virus, protection liver.So plant polyose is widely used medical field, meal already
In the popular life fields such as drink circle.
In the Patents applied at home, patent《A kind of preparation method of the lily polysaccharide of strengthen immunity》（Shen
Please number：200810097243.6, publication date：2008-09-24）A kind of lily polysaccharide of strengthen immunity is disclosed, what it was used
It is the method for ultrasonic wave pressurization composite centrifugal extraction, extraction efficiency is relatively low, obtains product purity relatively low；Further, since only one
Plant active ingredient, it is impossible to suitable for many situations, also no complementation mutually promotees effect, and function is compared with limitation.Patent《One kind improves immune
The preparation and its application of the purslane polysaccharide liposome of power》（Application number：201310502273.1, publication date：2014-01-22）
The preparation and its application of a kind of purslane polysaccharide liposome are disclosed, because it does not carry out ungrease treatment, the extract of acquisition
Composition is difficult to control to, but also additionally with the addition of the additives such as emulsifying agent, and step is complicated, it is difficult to realize.
The content of the invention
For drawbacks described above present in prior art, the present invention is intended to provide a kind of applied widely, effective ingredient it is many,
Effective ingredient has complementary functions, and mutually rush, extraction efficiency are high, high, lifting immunity that is being easily achieved is carried with polysaccharide to obtain product purity
Take thing and its manufacture method.
To achieve these goals, the present invention uses following technical scheme：One kind lifting immunity polysaccharide extract
Manufacture method, comprise the following steps：
1）The extraction of Shitake Mushroom P.E
1. raw material are prepared：Mushroom, the mushroom is gone to go rotten, root is removed, clean after toasted under 40 DEG C of -60 DEG C of environment, baking
Time is 2h-3h；Dehydration mushroom is done and shreds and be milled into powder by baking after terminating, after the sieve using 100-200 mesh, institute
That sifts out is as the mushroom dry powder of raw material；
2. the mushroom dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing mushroom dry powder；
3. the degreasing mushroom dry powder for 2. step being obtained is put into 50% ethanol water of its 20-40 times of quality multiple；Then will
50% ethanol water for adding degreasing mushroom dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 50% ethanol water that degreasing mushroom dry powder will be added is heated to 60 DEG C -70 DEG C, then with the work(of 500W-600W
Rate carries out ultrasonic activation extraction, and ultrasonic activation extraction time is 20min-30min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Shitake Mushroom P.E needed for obtaining, the Shitake Mushroom P.E contains the lentinan of 10%-30%, in brown powder；
2）The extraction of Coriolus Versicolor P.E.
1. raw material are prepared：Rainbow conk, the rainbow conk is gone to go rotten, root is removed, clean after toasted under 40 DEG C of -60 DEG C of environment, baking
Time is 2h-3h；Dehydration rainbow conk is done and shreds and be milled into powder by baking after terminating, and after the sieve using 50-80 mesh, is sieved
What is gone out is as the rainbow conk dry powder of raw material；
2. the rainbow conk dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing rainbow conk dry powder；
3. the degreasing rainbow conk dry powder for 2. step being obtained is put into 70% ethanol water of its 10-15 times of quality multiple；Then will
70% ethanol water for adding degreasing rainbow conk dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 70% ethanol water that degreasing rainbow conk dry powder will be added is heated to 40 DEG C -60 DEG C, then with the work(of 700W-800W
Rate carries out ultrasonic activation extraction, and ultrasonic activation extraction time is 10min-15min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Coriolus Versicolor P.E. needed for obtaining, the Coriolus Versicolor P.E. contains the coriolan of 30%-50%, in brown powder；
3）The extraction of Fructus lycii P.E
1. raw material are prepared：The fruit of Chinese wolfberry, the fruit of Chinese wolfberry is gone to go rotten, clean after toasted under 40 DEG C of -60 DEG C of environment, during baking
Between be 2h-3h；The dehydration fruit of Chinese wolfberry is done and shreds and be milled into powder by baking after terminating, after the sieve using 100-200 mesh, institute
That sifts out is as the fruit of Chinese wolfberry dry powder of raw material；
2. the fruit of Chinese wolfberry dry powder for 1. step being obtained is placed in Soxhlet extractor and uses the petroleum ether to carry out ungrease treatment for degreasing agent
After air-dry, that is, obtain degreasing fruit of Chinese wolfberry dry powder；
3. the degreasing fruit of Chinese wolfberry dry powder for 2. step being obtained is put into 80% ethanol water of its 10-20 times of quality multiple；Then
80% ethanol water that degreasing fruit of Chinese wolfberry dry powder will be added is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 80% ethanol water that degreasing fruit of Chinese wolfberry dry powder will be added is heated to 40 DEG C -60 DEG C, then with 800W-1000W's
Power carries out ultrasonic activation extraction, and ultrasonic activation extraction time is 15min-20min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Fructus lycii P.E needed for obtaining, the Fructus lycii P.E contains the Lycium barbarum polysaccharides of 20%-30%, in brown powder；
4）The extraction of Astragalus Root P.E
1. raw material are prepared：The Radix Astragali, toasts, baking after the Radix Astragali is removed aerial part, cleaned under 40 DEG C of -60 DEG C of environment
Time is 2h-3h；The dehydration Radix Astragali is done and shreds and be milled into powder by baking after terminating, after the sieve using 200-300 mesh, institute
That sifts out is as the Radix Astragali dry powder of raw material；
2. the Radix Astragali dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing Radix Astragali dry powder；
3. the degreasing Radix Astragali dry powder for 2. step being obtained is put into the pure water of its 8-12 times of quality multiple；Then degreasing will be added
The pure aqueous solution of Radix Astragali dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. the pure aqueous solution that degreasing Radix Astragali dry powder will be added is heated to 85 DEG C -90 DEG C, then with the power of 800W-1000W
Ultrasonic activation extraction is carried out, ultrasonic activation extraction time is 15min-20min；
5. the extract solution for 4. step being obtained carries out filtering solid treatment, then removes isolating protein, mucus by existing standard method
Matter constituents, surplus solution is spontaneously dried under closed environment, that is, Astragalus Root P.E needed for obtaining, the Astragalus Root P.E contains
The astragalus polyose of 50%-70%, in brown color or gray powdery；
5）Desired polysaccharide class extract is produced
By 1）~4）The various polysaccharide extracts for obtaining uniformly are mixed by following weight portion：Shitake Mushroom P.E 40-50 parts, rainbow conk
Extract 12-15 parts, Fructus lycii P.E 5-8 parts, Astragalus Root P.E 8-10 parts, that is, lifting immunity polysaccharide needed for obtaining
Extract.
The lifting immunity polysaccharide extract of above-mentioned manufacture method manufacture, is made up of the composition of following weight portion：It is fragrant
40-50 parts, Coriolus Versicolor P.E. 12-15 parts, Fructus lycii P.E 10-15 parts, Astragalus Root P.E 8-10 parts of mushroom extract.
Compared with the prior art, it is of the invention as a result of such scheme, with advantages below：Employ various different
Polysaccharide extract, wherein：Shitake Mushroom P.E can be as immunostimulant, while can resist numerous diseases includes artery atherosclerotic
Hardening, cancer, bacterium and virus infection etc.；Coriolus Versicolor P.E. dries resolving sputum effect, can treat tuberculosis and chronic bronchitis,
Unresolved or chronic hepatitis, is alternatively arranged as the immunotherapy medicaments of liver cancer patient；Fructus lycii P.E has immunological regulation, drop blood
Sugar, reducing blood lipid, anti-aging, antifatigue effect etc., also with blood vessel cholesterol is reduced, atherosclerosis, enhancing is regarded
Power, prevents glaucoma, brightens, moistens, skin care, reducing the effect such as pigment；Astragalus Root P.E has strengthen immunity, strengthens energy
Amount, antifatigue, anti-mutation, liver protection, suppress osteoclast effect, astragalus polyose also have reducing blood lipid, that is, reduce cholesterol and
The effect of triglycerides, increasing high density lipoprotein；Cardiovascular and cerebrovascular disease, such as atherosclerosis, hat can be prevented and treated
Shape arterial disease, peripheral angiopathy and hyperlipidemia etc..So-called immunologic hypofunction is a kind of pathological characters, more popular
Say, body immunity is exactly the ability that our body to be resisted infringement when in face of external infringement.It is such as ubiquitous
Bacterium, die-hard virus to your attack when, you directly determine whether you can catch an illness at the good and evil idea of body resistance ability.
Four kinds of effective ingredients of the invention also have antitumor, enhancing energy respectively except the ability of the lifting immunity with totally one property
Amount, lowering blood pressure and blood fat, defencive function sexual organ, anti-aging, antifatigue effect, the offer of these periphery functions can be human body band
Force environment is preferably immunized, making the immunity function of human body itself can more play its due effect, due to by test of many times
The rational proportion of acquisition can obtain powerful optimum proportioning, thus applied widely, effective ingredient of the invention it is many, it is effective into
Part is had complementary functions and mutually promote.All it is that the alcohol extracting affine to polysaccharide extract or alcohol-water mixture are extracted due to what is used, then
Auxiliary all carried out under closed, clean environment with ultrasonic wave extraction, and whole process, therefore extraction efficiency of the invention it is high,
Obtain product purity high；Due to disclosing whole technological process and formula, it is easily achieved Manufacturing Method of Products of the invention.
Specific embodiment
Embodiment 1：
One kind lifting immunity polysaccharide extract, is made up of the composition of following weight portion：40 parts of Shitake Mushroom P.E, rainbow conk are carried
Take 12 parts of thing, 10 parts of Fructus lycii P.E, 8 parts of Astragalus Root P.E.
The above-mentioned lifting immunity manufacture method of polysaccharide extract, comprises the following steps：
1）The extraction of Shitake Mushroom P.E
1. raw material are prepared：Mushroom, the mushroom is gone to go rotten, root is removed, clean after toasted under 40 DEG C of environment, baking time is
2h；Dehydration mushroom is done and shreds and be milled into powder by baking after terminating, and after the sieve using 100 mesh, being for being sifted out is made
It is the mushroom dry powder of raw material；
2. the mushroom dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing mushroom dry powder；
3. the degreasing mushroom dry powder for 2. step being obtained is put into 50% ethanol water of 20 times of its quality multiple；Then will add
50% ethanol water for having entered degreasing mushroom dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 50% ethanol water that degreasing mushroom dry powder will be added is heated to 60 DEG C, then carries out ultrasound with the power of 500W
Ripple vibration extraction, ultrasonic activation extraction time is 20min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Shitake Mushroom P.E needed for obtaining, the Shitake Mushroom P.E contains 10% lentinan, in brown powder；
2）The extraction of Coriolus Versicolor P.E.
1. raw material are prepared：Rainbow conk, the rainbow conk is gone to go rotten, root is removed, clean after toasted under 40 DEG C of environment, baking time is
2h；Dehydration rainbow conk is done and shreds and be milled into powder by baking after terminating, and after the sieve using 50 mesh, that is sifted out is conduct
The rainbow conk dry powder of raw material；
2. the rainbow conk dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing rainbow conk dry powder；
3. the degreasing rainbow conk dry powder for 2. step being obtained is put into 70% ethanol water of 10 times of its quality multiple；Then will add
70% ethanol water for having entered degreasing rainbow conk dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 70% ethanol water that degreasing rainbow conk dry powder will be added is heated to 40 DEG C, then carries out ultrasound with the power of 700W
Ripple vibration extraction, ultrasonic activation extraction time is 10min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Coriolus Versicolor P.E. needed for obtaining, the Coriolus Versicolor P.E. contains 30% coriolan, in brown powder；
3）The extraction of Fructus lycii P.E
1. raw material are prepared：The fruit of Chinese wolfberry, the fruit of Chinese wolfberry is gone to go rotten, clean after toasted under 40 DEG C of environment, baking time is
2h；The dehydration fruit of Chinese wolfberry is done and shreds and be milled into powder by baking after terminating, and after the sieve using 100 mesh, that is sifted out is
As the fruit of Chinese wolfberry dry powder of raw material；
2. the fruit of Chinese wolfberry dry powder for 1. step being obtained is placed in Soxhlet extractor and uses the petroleum ether to carry out ungrease treatment for degreasing agent
After air-dry, that is, obtain degreasing fruit of Chinese wolfberry dry powder；
3. the degreasing fruit of Chinese wolfberry dry powder for 2. step being obtained is put into 80% ethanol water of 10 times of its quality multiple；Then will
80% ethanol water for adding degreasing fruit of Chinese wolfberry dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 80% ethanol water that degreasing fruit of Chinese wolfberry dry powder will be added is heated to 40 DEG C, is then surpassed with the power of 800W
Acoustic vibration is extracted, and ultrasonic activation extraction time is 15min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Fructus lycii P.E needed for obtaining, the Fructus lycii P.E contains 20% Lycium barbarum polysaccharides, in brown powder；
4）The extraction of Astragalus Root P.E
1. raw material are prepared：The Radix Astragali, toasts, baking time after the Radix Astragali is removed aerial part, cleaned under 40 DEG C of environment
It is 2h；The dehydration Radix Astragali is done and shreds and be milled into powder by baking after terminating, and after the sieve using 200 mesh, that is sifted out is
As the Radix Astragali dry powder of raw material；
2. the Radix Astragali dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing Radix Astragali dry powder；
3. the degreasing Radix Astragali dry powder for 2. step being obtained is put into the pure water of 8 times of its quality multiple；Then degreasing will be added yellow
The pure aqueous solution of stilbene dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. the pure aqueous solution that degreasing Radix Astragali dry powder will be added is heated to 85 DEG C, and then carrying out ultrasonic wave with the power of 800W shakes
Dynamic to extract, ultrasonic activation extraction time is 15min；
5. the extract solution for 4. step being obtained carries out filtering solid treatment, then removes isolating protein, mucus by existing standard method
Matter constituents, surplus solution is spontaneously dried under closed environment, that is, Astragalus Root P.E needed for obtaining, the Astragalus Root P.E contains
50% astragalus polyose, in brown color or gray powdery；
5）Desired polysaccharide class extract is produced
By 1）~4）The various polysaccharide extracts for obtaining uniformly are mixed by following weight portion：40 parts of Shitake Mushroom P.E, rainbow conk are extracted
12 parts of thing, 5 parts of Fructus lycii P.E, 8 parts of Astragalus Root P.E, that is, lifting immunity polysaccharide extract needed for obtaining.
Embodiment 2：
One kind lifting immunity polysaccharide extract, is made up of the composition of following weight portion：450 parts of Shitake Mushroom P.E, rainbow conk
15 parts of extract, 15 parts of Fructus lycii P.E, 10 parts of Astragalus Root P.E.
The above-mentioned lifting immunity manufacture method of polysaccharide extract, comprises the following steps：
1）The extraction of Shitake Mushroom P.E
1. raw material are prepared：Mushroom, the mushroom is gone to go rotten, root is removed, clean after toasted under 60 DEG C of environment, baking time is
3h；Dehydration mushroom is done and shreds and be milled into powder by baking after terminating, and after the sieve using 200 mesh, being for being sifted out is made
It is the mushroom dry powder of raw material；
2. the mushroom dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing mushroom dry powder；
3. the degreasing mushroom dry powder for 2. step being obtained is put into 50% ethanol water of 40 times of its quality multiple；Then will add
50% ethanol water for having entered degreasing mushroom dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 50% ethanol water that degreasing mushroom dry powder will be added is heated to 70 DEG C, then carries out ultrasound with the power of 600W
Ripple vibration extraction, ultrasonic activation extraction time is 30min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Shitake Mushroom P.E needed for obtaining, the Shitake Mushroom P.E contains 30% lentinan, in brown powder；
2）The extraction of Coriolus Versicolor P.E.
1. raw material are prepared：Rainbow conk, the rainbow conk is gone to go rotten, root is removed, clean after toasted under 60 DEG C of environment, baking time is
3h；Dehydration rainbow conk is done and shreds and be milled into powder by baking after terminating, and after the sieve using 80 mesh, that is sifted out is conduct
The rainbow conk dry powder of raw material；
2. the rainbow conk dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing rainbow conk dry powder；
3. the degreasing rainbow conk dry powder for 2. step being obtained is put into 70% ethanol water of 15 times of its quality multiple；Then will add
70% ethanol water for having entered degreasing rainbow conk dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 70% ethanol water that degreasing rainbow conk dry powder will be added is heated to 60 DEG C, then carries out ultrasound with the power of 800W
Ripple vibration extraction, ultrasonic activation extraction time is 15min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Coriolus Versicolor P.E. needed for obtaining, the Coriolus Versicolor P.E. contains 50% coriolan, in brown powder；
3）The extraction of Fructus lycii P.E
1. raw material are prepared：The fruit of Chinese wolfberry, the fruit of Chinese wolfberry is gone to go rotten, clean after toasted under 60 DEG C of environment, baking time is
3h；The dehydration fruit of Chinese wolfberry is done and shreds and be milled into powder by baking after terminating, and after the sieve using 200 mesh, that is sifted out is
As the fruit of Chinese wolfberry dry powder of raw material；
2. the fruit of Chinese wolfberry dry powder for 1. step being obtained is placed in Soxhlet extractor and uses the petroleum ether to carry out ungrease treatment for degreasing agent
After air-dry, that is, obtain degreasing fruit of Chinese wolfberry dry powder；
3. the degreasing fruit of Chinese wolfberry dry powder for 2. step being obtained is put into 80% ethanol water of 20 times of its quality multiple；Then will
80% ethanol water for adding degreasing fruit of Chinese wolfberry dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 80% ethanol water that degreasing fruit of Chinese wolfberry dry powder will be added is heated to 60 DEG C, is then surpassed with the power of 1000W
Acoustic vibration is extracted, and ultrasonic activation extraction time is 20min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Fructus lycii P.E needed for obtaining, the Fructus lycii P.E contains 30% Lycium barbarum polysaccharides, in brown powder；
4）The extraction of Astragalus Root P.E
1. raw material are prepared：The Radix Astragali, toasts, baking time after the Radix Astragali is removed aerial part, cleaned under 60 DEG C of environment
It is 3h；The dehydration Radix Astragali is done and shreds and be milled into powder by baking after terminating, and after the sieve using 300 mesh, that is sifted out is
As the Radix Astragali dry powder of raw material；
2. the Radix Astragali dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing Radix Astragali dry powder；
3. the degreasing Radix Astragali dry powder for 2. step being obtained is put into the pure water of 12 times of its quality multiple；Then degreasing will be added yellow
The pure aqueous solution of stilbene dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. the pure aqueous solution that degreasing Radix Astragali dry powder will be added is heated to 90 DEG C, then carries out ultrasonic wave with the power of 1000W
Vibration extraction, ultrasonic activation extraction time is 20min；
5. the extract solution for 4. step being obtained carries out filtering solid treatment, then removes isolating protein, mucus by existing standard method
Matter constituents, surplus solution is spontaneously dried under closed environment, that is, Astragalus Root P.E needed for obtaining, the Astragalus Root P.E contains
70% astragalus polyose, in brown color or gray powdery；
5）Desired polysaccharide class extract is produced
By 1）~4）The various polysaccharide extracts for obtaining uniformly are mixed by following weight portion：50 parts of Shitake Mushroom P.E, rainbow conk are extracted
15 parts of thing, 8 parts of Fructus lycii P.E, 10 parts of Astragalus Root P.E, that is, lifting immunity polysaccharide extract needed for obtaining.
Present invention is particularly suitable for the hypoimmunity crowd of crowd's especially easy allergy of common drug of hypoimmunity,
Due to that will not develop immunity to drugs or other side effects, lifting immunity of the invention is adjusted with the suitable long-term taking of polysaccharide extract
Support.
The foregoing description of the disclosed embodiments, only for enabling professional and technical personnel in the field to realize or using this
Invention.Various modifications to these embodiments will be apparent for those skilled in the art, institute herein
The General Principle of definition can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore,
The present invention is not intended to be limited to the embodiments shown herein, and is to fit to special with principles disclosed herein and novelty
The consistent scope most wide of point.
Claims (2)
1. it is a kind of to lift the immunity manufacture method of polysaccharide extract, it is characterised in that to comprise the following steps：
1）The extraction of Shitake Mushroom P.E
1. raw material are prepared：Mushroom, the mushroom is gone to go rotten, root is removed, clean after toasted under 40 DEG C of -60 DEG C of environment, baking
Time is 2h-3h；Dehydration mushroom is done and shreds and be milled into powder by baking after terminating, after the sieve using 100-200 mesh, institute
That sifts out is as the mushroom dry powder of raw material；
2. the mushroom dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing mushroom dry powder；
3. the degreasing mushroom dry powder for 2. step being obtained is put into 50% ethanol water of its 20-40 times of quality multiple；Then will
50% ethanol water for adding degreasing mushroom dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 50% ethanol water that degreasing mushroom dry powder will be added is heated to 60 DEG C -70 DEG C, then with the work(of 500W-600W
Rate carries out ultrasonic activation extraction, and ultrasonic activation extraction time is 20min-30min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Shitake Mushroom P.E needed for obtaining, the Shitake Mushroom P.E contains the lentinan of 10%-30%, in brown powder；
2）The extraction of Coriolus Versicolor P.E.
1. raw material are prepared：Rainbow conk, the rainbow conk is gone to go rotten, root is removed, clean after toasted under 40 DEG C of -60 DEG C of environment, baking
Time is 2h-3h；Dehydration rainbow conk is done and shreds and be milled into powder by baking after terminating, and after the sieve using 50-80 mesh, is sieved
What is gone out is as the rainbow conk dry powder of raw material；
2. the rainbow conk dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing rainbow conk dry powder；
3. the degreasing rainbow conk dry powder for 2. step being obtained is put into 70% ethanol water of its 10-15 times of quality multiple；Then will
70% ethanol water for adding degreasing rainbow conk dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 70% ethanol water that degreasing rainbow conk dry powder will be added is heated to 40 DEG C -60 DEG C, then with the work(of 700W-800W
Rate carries out ultrasonic activation extraction, and ultrasonic activation extraction time is 10min-15min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Coriolus Versicolor P.E. needed for obtaining, the Coriolus Versicolor P.E. contains the coriolan of 30%-50%, in brown powder；
3）The extraction of Fructus lycii P.E
1. raw material are prepared：The fruit of Chinese wolfberry, the fruit of Chinese wolfberry is gone to go rotten, clean after toasted under 40 DEG C of -60 DEG C of environment, during baking
Between be 2h-3h；The dehydration fruit of Chinese wolfberry is done and shreds and be milled into powder by baking after terminating, after the sieve using 100-200 mesh, institute
That sifts out is as the fruit of Chinese wolfberry dry powder of raw material；
2. the fruit of Chinese wolfberry dry powder for 1. step being obtained is placed in Soxhlet extractor and uses the petroleum ether to carry out ungrease treatment for degreasing agent
After air-dry, that is, obtain degreasing fruit of Chinese wolfberry dry powder；
3. the degreasing fruit of Chinese wolfberry dry powder for 2. step being obtained is put into 80% ethanol water of its 10-20 times of quality multiple；Then
80% ethanol water that degreasing fruit of Chinese wolfberry dry powder will be added is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. 80% ethanol water that degreasing fruit of Chinese wolfberry dry powder will be added is heated to 40 DEG C -60 DEG C, then with 800W-1000W's
Power carries out ultrasonic activation extraction, and ultrasonic activation extraction time is 15min-20min；
5. the extract solution for 4. step being obtained is carried out filtering solid treatment, and surplus solution is spontaneously dried under closed environment,
Fructus lycii P.E needed for obtaining, the Fructus lycii P.E contains the Lycium barbarum polysaccharides of 20%-30%, in brown powder；
4）The extraction of Astragalus Root P.E
1. raw material are prepared：The Radix Astragali, toasts, baking after the Radix Astragali is removed aerial part, cleaned under 40 DEG C of -60 DEG C of environment
Time is 2h-3h；The dehydration Radix Astragali is done and shreds and be milled into powder by baking after terminating, after the sieve using 200-300 mesh, institute
That sifts out is as the Radix Astragali dry powder of raw material；
2. the Radix Astragali dry powder for 1. step being obtained is placed in Soxhlet extractor and uses after petroleum ether carries out ungrease treatment for degreasing agent
Air-dry, that is, obtain degreasing Radix Astragali dry powder；
3. the degreasing Radix Astragali dry powder for 2. step being obtained is put into the pure water of its 8-12 times of quality multiple；Then degreasing will be added
The pure aqueous solution of Radix Astragali dry powder is positioned in the ultrasonic activation machine for being provided with firing equipment；
4. the pure aqueous solution that degreasing Radix Astragali dry powder will be added is heated to 85 DEG C -90 DEG C, then with the power of 800W-1000W
Ultrasonic activation extraction is carried out, ultrasonic activation extraction time is 15min-20min；
5. the extract solution for 4. step being obtained carries out filtering solid treatment, then removes isolating protein, mucus by existing standard method
Matter constituents, surplus solution is spontaneously dried under closed environment, that is, Astragalus Root P.E needed for obtaining, the Astragalus Root P.E contains
The astragalus polyose of 50%-70%, in brown color or gray powdery；
5）Desired polysaccharide class extract is produced
By 1）~4）The various polysaccharide extracts for obtaining uniformly are mixed by following weight portion：Shitake Mushroom P.E 40-50 parts, rainbow conk
Extract 12-15 parts, Fructus lycii P.E 5-8 parts, Astragalus Root P.E 8-10 parts, that is, lifting immunity polysaccharide needed for obtaining
Extract.
2. the lifting immunity polysaccharide extract that manufacture method is manufactured according to claim 1, it is characterised in that：This is carried
Immunity polysaccharide extract is risen to be made up of the composition of following weight portion：Shitake Mushroom P.E 40-50 parts, Coriolus Versicolor P.E. 12-
15 parts, Fructus lycii P.E 10-15 parts, Astragalus Root P.E 8-10 parts.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110835378A (en) * | 2018-08-17 | 2020-02-25 | 金颖生物科技股份有限公司 | Ganoderma lucidum and yeast β -glucan composite polysaccharide composition |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2017290A1 (en) * | 2007-07-20 | 2009-01-21 | Caster | Polysaccharide extract of Lentinus and pharmaceutical, cosmetic or nutraceutical compositions comprising such an extract. |
| CN102908369A (en) * | 2012-10-24 | 2013-02-06 | 江苏博杰特机电有限公司 | Process for preparing shiitake extract |
| CN103467617A (en) * | 2013-09-18 | 2013-12-25 | 南京通泽农业科技有限公司 | Method for continuous counter-current ultrasonic extraction of high-purity astragalus polysaccharide |
| CN104371035A (en) * | 2014-10-24 | 2015-02-25 | 四川农业大学 | Method for quick pretreatment of raw materials of lentinan and ultrasonic assisted extraction of lentinan |
| CN104382929A (en) * | 2014-11-25 | 2015-03-04 | 高秀媛 | Medicine for removing blood garbage and vivotoxin and preparation method thereof |
| CN104829738A (en) * | 2015-05-12 | 2015-08-12 | 上海海洋大学 | Application of sargassum graminifolium polysaccharide extract in improvement of intestinal flora and prevention and treatment of diabetes |
| CN105030812A (en) * | 2015-06-24 | 2015-11-11 | 潘秀娟 | Compound fungal polysaccharide capsule |
-
2017
- 2017-04-01 CN CN201710213522.3A patent/CN106892996A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2017290A1 (en) * | 2007-07-20 | 2009-01-21 | Caster | Polysaccharide extract of Lentinus and pharmaceutical, cosmetic or nutraceutical compositions comprising such an extract. |
| CN102908369A (en) * | 2012-10-24 | 2013-02-06 | 江苏博杰特机电有限公司 | Process for preparing shiitake extract |
| CN103467617A (en) * | 2013-09-18 | 2013-12-25 | 南京通泽农业科技有限公司 | Method for continuous counter-current ultrasonic extraction of high-purity astragalus polysaccharide |
| CN104371035A (en) * | 2014-10-24 | 2015-02-25 | 四川农业大学 | Method for quick pretreatment of raw materials of lentinan and ultrasonic assisted extraction of lentinan |
| CN104382929A (en) * | 2014-11-25 | 2015-03-04 | 高秀媛 | Medicine for removing blood garbage and vivotoxin and preparation method thereof |
| CN104829738A (en) * | 2015-05-12 | 2015-08-12 | 上海海洋大学 | Application of sargassum graminifolium polysaccharide extract in improvement of intestinal flora and prevention and treatment of diabetes |
| CN105030812A (en) * | 2015-06-24 | 2015-11-11 | 潘秀娟 | Compound fungal polysaccharide capsule |
Non-Patent Citations (2)
| Title |
|---|
| 王振宇，卢卫红主编: "《天然产物分离技术》", 31 January 2012, 中国轻工业出版社 * |
| 蔡辉益，王晓红主编: "《饲料添加剂研究与应用新技术》", 31 January 2016, 中国农业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110835378A (en) * | 2018-08-17 | 2020-02-25 | 金颖生物科技股份有限公司 | Ganoderma lucidum and yeast β -glucan composite polysaccharide composition |
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