CN101284047A - Ginsenoside extract and its extraction method - Google Patents
Ginsenoside extract and its extraction method Download PDFInfo
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- CN101284047A CN101284047A CNA2007102004153A CN200710200415A CN101284047A CN 101284047 A CN101284047 A CN 101284047A CN A2007102004153 A CNA2007102004153 A CN A2007102004153A CN 200710200415 A CN200710200415 A CN 200710200415A CN 101284047 A CN101284047 A CN 101284047A
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- ginsenoside
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- fructus broussonetiae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
The invention discloses a ginsenoside extract and an extraction method thereof. The ginsenoside contains one or more effective ingredients selected from Rg1, Rb1, Rb2, Rd, Rc, Re, Rf, Rg2 and Rh1. The extraction method comprises the steps of: grinding dry Broussonetia papyrifera fruit, performing petroleum ether extraction or carbon dioxide supercritical extraction or water extraction under heating, and purifying with macroporous resin. The ginsenoside has remarkably nerve protecting, nerve nourishing, intelligence improving, and health promoting effects; has no toxicity to human body; and can be prepared into new drugs or natural health products for preventing or treating senile dementia.
Description
Technical field
The present invention relates to a kind of ginsenoside extract and extracting method thereof, belong to the Natural Medicine Chemistry research field.
Background technology
The ginsenoside is the general name of a saponins, how to extract to obtain from Radix Ginseng and Radix Notoginseng, but its content is extremely low, and the price of raw material is also higher simultaneously, makes that ginsenoside's extraction cost is very high.The ginsenoside has significant neuroprotective and neurotrophic activity; good nootropic effect is arranged; can be used for defying age, resisting fatigue, enhancing learning memory, treatment dementia etc.; simultaneously to the human body avirulence; have very considerable health-care effect, therefore become Medicine and the food supplement that common people pursue.
Fructus Broussonetiae is the dry mature fruit of Moraceae Broussonetia papyrifera Broussonetia papyrifera (L.) Vent., and is cheap, is conventional Chinese medicine, and China goes through an edition pharmacopeia and all records, and has the kidney invigorating liver heat removing, makes eye bright the diuretic effect.Be used for soreness of the waist and knees, the asthenia hectic fever due to YIN-deficiency, dizzy blurred vision, order is given birth to nebula film, edema distension." Mingyi Bielu " carries to top grade: sweet, cold, nontoxic.The big tonification of function cures mainly sexual impotence edema, QI invigorating, fills skin, makes eye bright, and clothes are not hungry, not old for a long time, make light of one's life by commiting suicide.In modern Chinese medicine was clinical, Fructus Broussonetiae was also usually used with other drug matching, treats multiple disease.But from Fructus Broussonetiae, extract the ginsenoside and yet there are no report.
Summary of the invention
The inventor adopts the research method of natural product, and separation and purification has obtained a series of ginsenoside's chemical compounds from Fructus Broussonetiae.
One of purpose of the present invention is to disclose a kind of ginsenoside extract; Another object of the present invention is the extracting method of open ginsenoside extract.
Technical scheme of the present invention: a kind of ginsenoside extract is characterized in that it is the ginsenoside's chemical compound that extracts from Fructus Broussonetiae.
In the above-mentioned ginsenoside extract, described ginsenoside's chemical compound effective ingredient contains one or more among Rg1, Rb1, Rb2, Rd, Rc, Re, Rf, Rg2 and the Rh1.
In the aforementioned ginsenoside extract, the structural formula of described ginsenoside's chemical compound effective ingredient Rg1 is
The extracting method of aforesaid ginsenoside extract is characterized in that: extracting ginsenoside's chemical compound from Fructus Broussonetiae is to extract by following step;
A, with dry Fructus Broussonetiae routinely extracting process extract, filter, make that fat content is no more than 5% in the filtering residue that obtains, the gained filtering residue is the A product;
B, the A product are extracted according to a conventional method, gained extracting solution macroporous resin treatment, first water eluting, reuse alcoholic solution eluting is collected the ethanol eluent, the B product;
C, the B product are concentrated, drying obtains ginsenoside extract.
In the aforesaid ginsenoside extract extracting method, described step a can for, dry Fructus Broussonetiae through grinding, press 1g: the solid-to-liquid ratio of 3～5ml is with petroleum ether heating and refluxing extraction 4 times, each 1.5 hours, filtration, the filtering residue that obtains is the A product.
In the aforesaid ginsenoside extract extracting method, described step a can also for, dry Fructus Broussonetiae through grinding, is used CO
2The supercritical fluid extraction method extracts, and filters, and makes that fat content is no more than 5% in the filtering residue that obtains, and the gained filtering residue is the A product.
In the aforesaid ginsenoside extract extracting method, conventional extracting method is described in the step b, and the A product are decocted with water 3 times, adds 2～3 times of water gagings at every turn, decocts 2 hours at every turn, filters, and merging filtrate gets extracting solution.
In the aforesaid ginsenoside extract extracting method, the extracting solution described in the step b is handled with D-101 type macroporous adsorbent resin
In the aforesaid ginsenoside extract extracting method, alcoholic solution described in the step b is that volumetric concentration is the alcoholic solution of 60-80%.
Owing to adopted technique scheme, the present invention that following advantage is arranged:
(1) the present invention has reduced ginsenoside's production cost.Compare with the existing technology of extracting the ginsenoside from other raw material the inside such as Radix Ginseng or Radix Notoginseng, it is raw material that the present invention adopts Fructus Broussonetiae, because the price of Fructus Broussonetiae is well below the price of raw materials such as Radix Ginseng, so the present invention greatly reduces ginsenoside's production cost.
(2) the invention provides the preparation method that the industry that is suitable for is produced, have good market prospect.
(3) Fructus Broussonetiae is the dry mature fruit of Moraceae Broussonetia papyrifera Broussonetia papyrifera (L.) Vent., the industry production of extract of the present invention can make market being in great demand to Fructus Broussonetiae, Broussonetia papyrifera all can be planted in most of area in the whole nation, so the present invention also provides a chance of shaking off poverty and setting out on the road to prosperity by the plantation Broussonetia papyrifera for the peasant.
(4) extract that contains the ginsenoside that has adopted technique scheme to obtain has tangible neurotrophy and nerve injury protection activity.
The specific embodiment
Ginsenoside extract of the present invention is the ginsenoside's chemical compound that extracts from Fructus Broussonetiae, and its effective ingredient contains one or more among Rg1, Rb1, Rb2, Rd, Rc, Re, Rf, Rg2 and the Rh1, and wherein the structural formula of Rg1 is
Extraction step is:
A, with dry Fructus Broussonetiae routinely extracting process extract, filter, make that fat content is no more than 5% in the filtering residue that obtains, the gained filtering residue is the A product;
B, the A product are extracted according to a conventional method, gained extracting solution macroporous resin treatment, first water eluting, reuse alcoholic solution eluting is collected the ethanol eluent, the B product;
C, the B product are concentrated, drying obtains ginsenoside extract.
Embodiment 1: extract ginsenoside's chemical compound by following step from Fructus Broussonetiae.
A, get dry Fructus Broussonetiae 100kg and grind, with the Fructus Broussonetiae after pulverizing with 400l petroleum ether heating and refluxing extraction 4 times, each 1.5 hours, filter, the filtering residue that obtains is the A product;
B, A product add water 300l and decoct 3 times, and each 2 hours, filtration, merging filtrate, filtrate is handled with D-101 type macroporous adsorbent resin, uses the distilled water eluting earlier, uses 60% alcoholic solution eluting then, collects alcoholic solution eluting part, gets the B product;
C, the B product are concentrated, drying obtains ginsenoside extract.
The effective ingredient of ginsenoside extract contains one or more among Rg1, Rb1, Rb2, Rd, Rc, Re, Rf, Rg2 and the Rh1.
Embodiment 2: extract ginsenoside's chemical compound by following step from Fructus Broussonetiae.
A, get dry Fructus Broussonetiae 100kg and grind, with the Fructus Broussonetiae after pulverizing with 300l petroleum ether heating and refluxing extraction 4 times, each 1.5 hours, filter, the filtering residue that obtains is the A product;
B, A product add water 200l and decoct 3 times, and each 2 hours, filtration, merging filtrate, filtrate is handled with D-101 type macroporous adsorbent resin, uses the distilled water eluting earlier, uses 70% alcoholic solution eluting then, collects alcoholic solution eluting part, gets the B product;
C, the B product are concentrated, drying obtains ginsenoside extract.
The effective ingredient of ginsenoside extract contains one or more among Rg1, Rb1, Rb2, Rd, Rc, Re, Rf, Rg2 and the Rh1.
Embodiment 3: extract ginsenoside's chemical compound by following step from Fructus Broussonetiae.
A, get dry Fructus Broussonetiae 100kg and grind, with the Fructus Broussonetiae after pulverizing with 500l petroleum ether heating and refluxing extraction 4 times, each 1.5 hours, filter, the filtering residue that obtains is the A product;
B, A product add water 300l and decoct 3 times, and each 2 hours, filtration, merging filtrate, filtrate is handled with D-101 type macroporous adsorbent resin, uses the distilled water eluting earlier, uses 80% alcoholic solution eluting then, collects alcoholic solution eluting part, gets the B product;
C, the B product are concentrated, drying obtains ginsenoside extract.
The effective ingredient of ginsenoside extract contains one or more among Rg1, Rb1, Rb2, Rd, Rc, Re, Rf, Rg2 and the Rh1.
Embodiment 4: extract ginsenoside's chemical compound by following step from Fructus Broussonetiae.
A, get dry Fructus Broussonetiae 100kg and grind, the Fructus Broussonetiae CO after pulverizing
2The supercritical fluid extraction method extracts, and filters, and makes that fat content is no more than 5% in the filtering residue that obtains, and the gained filtering residue is the A product;
B, A product add water 300l and decoct 3 times, and each 2 hours, filtration, merging filtrate, filtrate is handled with D-101 type macroporous adsorbent resin, uses the distilled water eluting earlier, uses 60% alcoholic solution eluting then, collects alcoholic solution eluting part, gets the B product;
C, the B product are concentrated, drying obtains ginsenoside extract.
The effective ingredient of ginsenoside extract contains one or more among Rg1, Rb1, Rb2, Rd, Rc, Re, Rf, Rg2 and the Rh1.
Embodiment 5: extract ginsenoside's chemical compound by following step from Fructus Broussonetiae.
A, get dry Fructus Broussonetiae 100kg and grind, the Fructus Broussonetiae CO after pulverizing
2The supercritical fluid extraction method extracts, and filters, and makes that fat content is no more than 5% in the filtering residue that obtains, and the gained filtering residue is the A product;
B, A product add water 250l and decoct 3 times, and each 2 hours, filtration, merging filtrate, filtrate is handled with D-101 type macroporous adsorbent resin, uses the distilled water eluting earlier, uses 70% alcoholic solution eluting then, collects alcoholic solution eluting part, gets the B product;
C, the B product are concentrated, drying obtains ginsenoside extract.
The effective ingredient of ginsenoside extract contains one or more among Rg1, Rb1, Rb2, Rd, Rc, Re, Rf, Rg2 and the Rh1.
Embodiment 6: extract ginsenoside's chemical compound by following step from Fructus Broussonetiae.
A, get dry Fructus Broussonetiae 100kg and grind, the Fructus Broussonetiae CO after pulverizing
2The supercritical fluid extraction method extracts, and filters, and makes that fat content is no more than 5% in the filtering residue that obtains, and the gained filtering residue is the A product;
B, A product add water 200l and decoct 3 times, and each 2 hours, filtration, merging filtrate, filtrate is handled with D-101 type macroporous adsorbent resin, uses the distilled water eluting earlier, uses 80% alcoholic solution eluting then, collects alcoholic solution eluting part, gets the B product;
C, the B product are concentrated, drying obtains ginsenoside extract.
The effective ingredient of ginsenoside extract contains one or more among Rg1, Rb1, Rb2, Rd, Rc, Re, Rf, Rg2 and the Rh1.
Claims (9)
1. ginsenoside extract, it is characterized in that: it is the ginsenoside's chemical compound that extracts from Fructus Broussonetiae.
2. ginsenoside extract according to claim 1, it is characterized in that: this ginsenoside's chemical compound effective ingredient contains one or more among Rg1, Rb1, Rb2, Rd, Rc, Re, Rf, Rg2 and the Rh1.
3. as ginsenoside extract as described in the claim 2, it is characterized in that: the structural formula of described ginsenoside's chemical compound effective ingredient Rg1 is:
4. as the extracting method of claim 1,2 or 3 described ginsenoside extracts, it is characterized in that: extracting ginsenoside's chemical compound from Fructus Broussonetiae is to extract by following step;
A, with dry Fructus Broussonetiae routinely extracting process extract, filter, make that fat content is no more than 5% in the filtering residue that obtains, the gained filtering residue is the A product;
B, the A product are extracted according to a conventional method, gained extracting solution macroporous resin treatment, first water eluting, reuse alcoholic solution eluting is collected the ethanol eluent, the B product;
C, the B product are concentrated, drying obtains ginsenoside extract.
5. the extracting method of ginsenoside extract as claimed in claim 4 is characterized in that: described step a is, with dry Fructus Broussonetiae through grinding, press 1g: petroleum ether heating and refluxing extraction 4 times of the solid-to-liquid ratio of 3～5ml, each 1.5 hours, filter, the filtering residue that obtains is the A product.
6. the extracting method of ginsenoside extract as claimed in claim 4 is characterized in that: described step a is, with dry Fructus Broussonetiae through grinding, extract with CO2 supercritical fluid extraction method, filter, make that fat content is no more than 5% in the filtering residue that obtains, the gained filtering residue is the A product.
7. the extracting method of ginsenoside extract as claimed in claim 4, it is characterized in that: conventional extracting method is described in the step b, and the A product are decocted with water 3 times, adds 2～3 times of water gagings at every turn, decocts 2 hours at every turn, filters, merging filtrate gets extracting solution.
8. the extracting method of ginsenoside extract as claimed in claim 4 is characterized in that: the D-101 type macroporous adsorbent resin processing of the extracting solution described in the step b.
9. the extracting method of ginsenoside extract as claimed in claim 4, it is characterized in that: alcoholic solution described in the step b is that volumetric concentration is the alcoholic solution of 60-80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNA2007102004153A CN101284047A (en) | 2007-04-09 | 2007-04-09 | Ginsenoside extract and its extraction method |
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| CNA2007102004153A CN101284047A (en) | 2007-04-09 | 2007-04-09 | Ginsenoside extract and its extraction method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101862370A (en) * | 2010-05-04 | 2010-10-20 | 严希海 | Separation method of broussonetia active ingredients |
| CN102649806A (en) * | 2011-02-25 | 2012-08-29 | 苏州宝泽堂医药科技有限公司 | Preparation method of pedunculoside |
| CN103316083A (en) * | 2013-07-12 | 2013-09-25 | 上海相宜本草化妆品股份有限公司 | Preparation method for paper mulberry fruit extracts and application of paper mulberry fruit extracts in skin whitening |
| CN106138142A (en) * | 2016-08-16 | 2016-11-23 | 集安市清河人参交易市场有限公司 | A kind of extracting method of wild ginseng saponin |
| CN106361792A (en) * | 2016-11-02 | 2017-02-01 | 无锡西玛生物科技有限公司 | Improved production method of black ginseng |
| CN106900856A (en) * | 2017-03-07 | 2017-06-30 | 吉林大学 | The balloonflower root fermented ginseng Yoghourt and its production method of a kind of green |
| CN110117306A (en) * | 2018-02-07 | 2019-08-13 | 北京北林先进生态环保技术研究院有限公司 | Obtain ginsenoside Rg2Method and system |
-
2007
- 2007-04-09 CN CNA2007102004153A patent/CN101284047A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101862370A (en) * | 2010-05-04 | 2010-10-20 | 严希海 | Separation method of broussonetia active ingredients |
| CN101862370B (en) * | 2010-05-04 | 2012-02-01 | 山东中科生物科技股份有限公司 | Separation method of broussonetia active ingredients |
| CN102649806A (en) * | 2011-02-25 | 2012-08-29 | 苏州宝泽堂医药科技有限公司 | Preparation method of pedunculoside |
| CN103316083A (en) * | 2013-07-12 | 2013-09-25 | 上海相宜本草化妆品股份有限公司 | Preparation method for paper mulberry fruit extracts and application of paper mulberry fruit extracts in skin whitening |
| CN103316083B (en) * | 2013-07-12 | 2014-12-10 | 上海相宜本草化妆品股份有限公司 | Preparation method for paper mulberry fruit extracts and application of paper mulberry fruit extracts in skin whitening |
| CN106138142A (en) * | 2016-08-16 | 2016-11-23 | 集安市清河人参交易市场有限公司 | A kind of extracting method of wild ginseng saponin |
| CN106361792A (en) * | 2016-11-02 | 2017-02-01 | 无锡西玛生物科技有限公司 | Improved production method of black ginseng |
| CN106900856A (en) * | 2017-03-07 | 2017-06-30 | 吉林大学 | The balloonflower root fermented ginseng Yoghourt and its production method of a kind of green |
| CN110117306A (en) * | 2018-02-07 | 2019-08-13 | 北京北林先进生态环保技术研究院有限公司 | Obtain ginsenoside Rg2Method and system |
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Open date: 20081015 |