CN104744532A - Extraction and refining method of sapindusmu korossi saponin - Google Patents
Extraction and refining method of sapindusmu korossi saponin Download PDFInfo
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- CN104744532A CN104744532A CN201510154289.7A CN201510154289A CN104744532A CN 104744532 A CN104744532 A CN 104744532A CN 201510154289 A CN201510154289 A CN 201510154289A CN 104744532 A CN104744532 A CN 104744532A
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- sapindoside
- resin
- korossi
- sapindusmu
- saponin
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Abstract
The invention relates to an extraction and refining method of sapindusmu korossi saponin. According to the method, effective constituents of sapindusmu korossi are separated and purified by utilizing strong-polar macropore adsorbent resin; by taking tsapindusmu korossi as a raw material, a high-quality sapindusmu korossi saponin extractive can be separated and purified from a sapindusmu korossi extracting solution only by a simple 'adsorption-desorption' one-step process; the separation effect is good; and the product purity is high.
Description
Technical field
The present invention relates to the Extraction and isolation of effective constituent in vegetable Chinese herbal medicine, particularly a kind of method of Hydrolysis kinetics of sapindoside.
Background technology
Seed of Chinese Soapberry (Sapindusmu korossi) is a kind of deciduous tree of Sapindaceae, Sapindus, is distributed in various places on the south Huaihe River.Containing antibacterial, sterilization, anti-inflammatory and natural nutrition conditioning ingredients in soapberry extract, have dandruff-removing itching-relieve effect, its surface-active substance is a kind of raw material being particularly suitable for preparing shampoo.Ancient Chinese various doctor nationality also does detailed record to the cleaning of Seed of Chinese Soapberry, medical functions.Compendium of Materia Medica records Seed of Chinese Soapberry heat-clearing, eliminate the phlegm, disappear long-pending, desinsection, and sub-skin has wind improving eyesight, effects such as spot of washing one's face.Seed of Chinese Soapberry class pharmaceutical prod DEVELOPMENT PROSPECT is wide.
Containing a large amount of saponin constituent in the arillus of Seed of Chinese Soapberry, except there is good whipability and detergency ability, also there is the effects such as antibacterial and antipruritic, can be used in natural shampoo and various cleaning and protecting skin makeup as natural active matter, also can be used to smelting and treat the ringworm of the foot and ringworm.Soapberry extract joins in skin care product and makeup by Tono, Masayuki, serves certain protection skin and the dermopathic effect of control; To Seed of Chinese Soapberry, Sun Jieru etc. show that the character of activity and compound system thereof is studied; Zhang Minjie etc. have developed a kind of natural surface active agent containing Seed of Chinese Soapberry.
Sapindoside or well farm chemical emulgent, all have good killing effect to cotton aphid, red spider and sweet potato Jinhua worm etc.The research such as Huang Hui-chi finds that saponin content is that 10ppm just can kill apple worm 70 ~ 100% etc. in Seed of Chinese Soapberry wormer.
Less to the extraction and separation technology aspect report both domestic and external of Seed of Chinese Soapberry.At present, the preparation method of sapindoside mainly contains Sun Jieru and adopts extraction using alcohol one n-butanol extraction separating technology, and obtain the thick product of flaxen sapindoside, this method solvent load is large, and product purity is low.Rich thick once grade adopts extraction using alcohol one solvent extraction and separation technology, obtain faint yellow finished product, but ethanol consumption is comparatively large, cumbersome, and productive rate is not high.Zhang Minjie etc. adopt the method for water extraction, and obtaining working substance content is 15 ~ 28% aqueous products.
Macroporous adsorbent resin is the novel macromolecular compound with macroporous structure of a class, it is the parting material that a kind of adsorptivity combines with screening property principle, it, for the polishing purification of Radix Ginseng total saponins, soybean saponin, total saponins from Cornus officinalis etc., can remove the impurity such as water colo(u)r, polysaccharide and inorganic salt.
Summary of the invention
The object of this invention is to provide a kind of method of Hydrolysis kinetics of sapindoside.Present invention achieves only " adsorption-desorption " single stage method, technique is simple, produces the total saponin of sapindusmukerossi obtaining higher degree and productive rate.Cost of the present invention is low, saponin(e loss is little, quality product is high.
The present invention includes following step:
1) by Seed of Chinese Soapberry raw material pulverizing, the ethanolic soln diacolation with 25 ~ 95% or ethanolic soln heat are extracted, and after extracting solution reclaims ethanol, with the water dilution of 4 ~ 5 times, filter to obtain clear aqueous solution;
2) clear aqueous solution is passed in the resin column that strong Polar Adsorbent Resin is housed and adsorb, the water washing of pH2 ~ 8, the ethanolic soln wash-out of 25 ~ 95%; Absorption, washing, desorb flow velocity be 0.2 ~ 5 bed volume/hour;
3) stripping liquid is through concentrating under reduced pressure, vacuum-drying, obtains white products.
Described resin is strong polar quaternary ammonium base polymeric adsorbent; Described raw material weight and the volume ratio of resin are 1:1 ~ 5; Described resin column footpath and post height are than being 1:1 ~ 20.
The advantage that the present invention compared with prior art has is: the composition of the present invention according to Seed of Chinese Soapberry ingredient and the constructional feature of sapindoside, select the multi-functional polymeric adsorbent of the strong polarity of macropore with a certain amount of commutative function base---strong polar quaternary ammonium base polymeric adsorbent (ADS-2), the physical adsorption of the Van der Waals force on this resin dependence surface, the chemisorption of function base, the adsorption by hydrogen bond of functional group, and the synergy of three is adsorbed the chemical composition in extracting solution.Compare with traditional solvent extration, technique is simple, do not use toxicity and volatile organic solvent in technical process, safe and reliable, production cost is lower.The present invention compared with other polymeric adsorbent or chromatography, do not need solvent extraction or desalination bleaching resin with the use of, enormously simplify production technique, improve productive rate, a step can obtain that color and luster is good, content is high, stay-in-grade product.
Embodiment
Embodiment 1
Pulverized by 20 grams of Seed of Chinese Soapberry pericarps, the aqueous ethanolic solution thermal backflow of 80% concentration, extracting solution is diluted with water to 100-120ml, the clear aqueous solution of filtration after reclaiming ethanol.The aqueous solution obtained is passed into the resin column (post footpath 15mm, is equipped with 50m1 resin) that ADS-2 resin is housed, with the water washing of the pH=6 of 100ml, with the ethanol desorb of 20,0m1 50%, absorption, washing, desorb flow velocity be 0.5 times of bed volume/hour.Namely stripping liquid obtains white powder sapindoside extract 1.72g through concentrating under reduced pressure, vacuum-drying, pulverizing, and productive rate is 8.60%.
Embodiment 2
Pulverized by 20 grams of Seed of Chinese Soapberry, the aqueous ethanolic solution thermal backflow of 80% concentration, extracting solution is diluted with water to 100-120ml, the clear aqueous solution of filtration after reclaiming ethanol.The aqueous solution obtained is passed into the resin column (post footpath 15mm, is equipped with 50m1 resin) that ADS-2 resin is housed, with the water washing of 100ml, with the ethanol desorb of 20,0m1 70%, absorption, washing, desorb flow velocity be 1 times of bed volume/hour.Namely stripping liquid obtains white powder sapindoside through concentrating under reduced pressure, vacuum-drying, pulverizing and catches and get thing 1.62g, and productive rate is 8.10%.
Embodiment 3
Pulverized by 10kg Seed of Chinese Soapberry, the aqueous ethanolic solution thermal backflow of 80% concentration, extracting solution is diluted with water to 60L, the clear aqueous solution of filtration after reclaiming ethanol.The aqueous solution obtained is passed into the resin column (post footpath 500mm, is equipped with 20L resin) that ADS-2 resin is housed, with the water washing of 40L, with the ethanol desorb of 100L 50%, absorption, washing, desorb flow velocity be 1 times of bed volume/hour.Namely stripping liquid obtains white powder sapindoside extract 823g through concentrating under reduced pressure, vacuum-drying, pulverizing, and productive rate is 8.23%.
Claims (4)
1. a method for the Hydrolysis kinetics of sapindoside, is characterized in that it comprises the steps:
1) by Seed of Chinese Soapberry raw material pulverizing, extract by ethanolic soln diacolation or ethanolic soln heat, after extracting solution reclaims ethanol, with the water dilution of 4 ~ 5 times, filter to obtain clear aqueous solution;
2) clear aqueous solution is passed in the resin column that strong Polar Adsorbent Resin is housed and adsorb, the water washing of pH 2 ~ 8, the ethanolic soln wash-out of 25 ~ 95%; Absorption, washing, desorb flow velocity be 0.2 ~ 5 bed volume/hour;
3) stripping liquid is through concentrating under reduced pressure, vacuum-drying, obtains product.
2. the method for the Hydrolysis kinetics of a kind of sapindoside according to claim 1, is characterized in that described resin is strong polar quaternary ammonium base polymeric adsorbent.
3. the method for the Hydrolysis kinetics of a kind of sapindoside according to claim 1 and 2, is characterized in that the volume of described raw material weight and resin is 1:1 ~ 5.
4. the extracting method of sapindoside according to claim 1, is characterized in that described resin column footpath and post height are than being 1:1 ~ 20.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510154289.7A CN104744532A (en) | 2015-04-03 | 2015-04-03 | Extraction and refining method of sapindusmu korossi saponin |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510154289.7A CN104744532A (en) | 2015-04-03 | 2015-04-03 | Extraction and refining method of sapindusmu korossi saponin |
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| CN104744532A true CN104744532A (en) | 2015-07-01 |
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| CN201510154289.7A Pending CN104744532A (en) | 2015-04-03 | 2015-04-03 | Extraction and refining method of sapindusmu korossi saponin |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106831931A (en) * | 2017-01-23 | 2017-06-13 | 厦门大学 | A kind of method that two-step method prepares sapindoside |
| CN113546574A (en) * | 2021-06-23 | 2021-10-26 | 广东菁萃生物科技有限公司 | High-stability saponin composition and application thereof |
-
2015
- 2015-04-03 CN CN201510154289.7A patent/CN104744532A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106831931A (en) * | 2017-01-23 | 2017-06-13 | 厦门大学 | A kind of method that two-step method prepares sapindoside |
| CN113546574A (en) * | 2021-06-23 | 2021-10-26 | 广东菁萃生物科技有限公司 | High-stability saponin composition and application thereof |
| CN113546574B (en) * | 2021-06-23 | 2022-04-12 | 广东菁萃生物科技有限公司 | High-stability saponin composition and application thereof |
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Application publication date: 20150701 |