CN1066055C - Productive method for extracting flavonoid compound extract or powder from bamboo leaf - Google Patents

Productive method for extracting flavonoid compound extract or powder from bamboo leaf Download PDF

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CN1066055C
CN1066055C CN 98104564 CN98104564A CN1066055C CN 1066055 C CN1066055 C CN 1066055C CN 98104564 CN98104564 CN 98104564 CN 98104564 A CN98104564 A CN 98104564A CN 1066055 C CN1066055 C CN 1066055C
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bamboo
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flavonoids
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张英
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浙江农业大学
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本发明属于一种从植物中提取黄酮类化合物,尤其是从竹叶中提取黄酮类化合物浸膏或粉剂的生产方法。 The present invention pertains to a flavonoid extracted from plants, in particular production process to extract flavonoids from the extract or powder of bamboo. 以竹叶为原料,通过提取、浓缩、分离、精制、或再干燥所得的产品,含有大量的竹叶黄酮糖苷,及植物酚酸、蒽醌类、萜类内酯等化合物。 In bamboo as a raw material, by extraction, concentration, separation, purification, and then drying the resulting product, or, it contains a lot of bamboo flavone glycosides, phenolic acids and plant, anthraquinone, terpene lactone compounds. 该制剂具有优良的抗自由基、抗氧化、抗衰老和降血脂等作用,并带有典型的竹子芳香,可广泛地应用于各种老年功能性食品和医药保健品。 The formulation has an excellent anti-free radical, anti-oxidation, anti-aging and hypolipidemic action, and with a typical aromatic bamboo, can be widely applied to various functional foods and pharmaceutical elderly health products. 该制剂是用低浓度的低级醇为溶剂热回流提取,用中级醇液~液萃取进行精制。 The preparation is extracted with a lower alcohol, a low concentration of heat at reflux solvent, was purified ~ Intermediate liquid extraction with an alcohol solution. 生产过程安全、简便,无污染,易于控制,适于大规模的工业化生产,同时还具有黄酮提取率高、纯度好等特点。 The production process is safe, simple, clean, easy to control, suitable for large-scale industrial production, and also has flavonoids extraction rate, good purity and other characteristics.

Description

从竹叶中提取黄酮类化合物浸膏或粉剂的生产方法 Extraction production of flavonoids from the extract or powder of bamboo

本发明涉及一种从植物中提取黄酮类化合物的生产方法,尤其是从从竹叶中提取黄酮类化合物浸膏或粉剂的生产方法。 The present invention relates to a method for the production of flavonoids extracted from plants, especially from the production of the extraction of flavonoids from the extract or powder of bamboo.

生物黄酮广泛存在于植物界,是许多中草药的有效成分。 Bioflavonoids widespread in the plant kingdom, is the active ingredient in many Chinese herbal medicine. 除了从银杏叶、法国松树皮、日本杜仲中提取黄酮类制剂以外,葛根、甘草、山楂、茶叶、柿叶、马尾松针及松实、桂皮、合欢皮、枸杞根皮茎叶、石斛、虫草、首乌、桔皮、五味子、枣、花生壳、稻根、茄杆等内含的生物黄酮均具有开发价值。 In addition to flavonoids extracted from the preparation of Ginkgo biloba, French pine bark, other than Japan gutta, kudzu, licorice, hawthorn, tea, persimmon leaves, pine needles and pine real, cinnamon, Albizia, wolfberry root bark of stems and leaves, Dendrobium, Chinese caterpillar fungus, containing the bioflavonoids Radix, orange peel, Schisandra, dates, peanut shells, rice root, eggplant rods, etc. all have development value. 以当今生产和使用规模最大的银杏黄酮为例,其生产工艺大致为以下3种类型:(1)水蒸汽蒸馏法:将银杏叶干燥、粉碎后,100~120℃水蒸汽蒸馏,蒸馏液冷却后,用非极性大孔树脂(或其他吸附剂)吸附,然后用乙醇洗脱,蒸干即得粉状结晶。 In today's largest production and use of flavonoids as an example, its production process is generally the following three types: (1) steam distillation: The Ginkgo biloba dried, crushed, 100 ~ 120 ℃ steam distillation, the distillate was cooled after non-polar macroporous resin (or other adsorbent) is adsorbed, and then eluted with ethanol, evaporated to dryness to obtain powdery crystals. 该方法设备简单,但提取率低,现已很少采用。 The apparatus is simple, but the extraction rate, is now rarely used.

(2)有机溶剂萃取:这是目前国内外使用最广泛的方法,其提取工艺的专利也较多[从银杏叶中提取黄酮类化合物的方法,李兆龙,中草药,1992,14(6):5-6],可归纳为两种形式。 (2) organic solvent extraction: This is the method most widely used at home and abroad, which patent is greater extraction process [Extraction of flavonoids from Ginkgo leaves, Lizhao Long, herbs, 1992,14 (6): 5 -6], it can be summarized into two forms. 一种是制备粗提物:把银杏叶干燥、粉碎后用有机溶剂(如乙醇或丙酮)提取,过滤、减压浓缩回收溶剂后,即得银杏浸膏。 One is the preparation of crude extracts of: Ginkgo biloba dried, pulverized and extracted with an organic solvent (e.g. ethanol or acetone), filtered, and concentrated under reduced pressure to recover the solvent, to give the ginkgo extract. 这种方法生产设备简单,产品得率高(约12~14%),但含大量的杂质,通常呈棕黑色,制品的总黄酮含量为7~10%。 This method is simple production equipment, high product yield (about 12 to 14%), but containing a large amount of impurities, typically brownish-black, total flavonoids article is 7 to 10%. 另一种是制备精提物:这类方法通常在粗提物的基础上进一步精制,常用的精制方法有:液~液萃取(例如用二氯甲烷、氯仿、石油醚或苯脱脂;或用丁酮、丙酮-丁酮或乙酸乙酯萃取),沉淀法(用氨水或氢氧化铅沉淀)和吸附~解析法(用大孔树脂、硅藻土或硅胶等吸附剂吸附,乙醇洗脱)。 Another is to prepare fine extracts: generally further purified by such methods, commonly used method of refining the crude extract on the basis of: - liquid-liquid extraction (e.g. methylene chloride, chloroform, benzene or petroleum ether, defatted; or with butanone, acetone - extracted with ethyl acetate or methyl ethyl ketone), precipitation (with ammonia or lead hydroxide precipitation) and adsorption - analytical method (with a macroporous resin, diatomaceous earth or silica gel adsorbent, eluting with ethanol) . 再通过无水硫酸钠干燥、过滤、减压蒸馏除去溶剂后,即可得精提物。 Then dried over anhydrous sodium sulfate, filtered and distilled to remove the solvent under reduced pressure to obtain refined extract. 一般为黄褐色粉末,得率约1~3%,总黄酮含量约为20~26%。 Generally as a tan powder, yield from about 1 to 3%, of the total flavonoid content from about 20 to 26%.

(3)超临界流体萃取:近10年来,用该技术提取天然植物中的药用有效成分越来越受到关注,它和上述工艺相比,具有提取效率高、无溶剂残留、活性成分不易被分解和破坏等优点。 (3) SFE: the past 10 years, the natural extract active ingredients of medicinal plants with more and more attention to the technique, the process described above and which, compared with a high extraction efficiency, no solvent residue, the active ingredient is not easily advantages decomposition and destruction. 欧洲专利EP0308675(1988)报道了用超临界CO2提取银杏叶中有效成分的方法,国内近年来也在这方面做了不少工作,但大多处在实验室研究阶段,未见有工业化应用的报道。 European Patent EP0308675 (1988) reported a method to extract the active ingredients of gingko leaves with supercritical CO2, also in this country in recent years has done a lot of work, but mostly in the laboratory research stage, there are no reports of industrial applications .

竹子是禾本科(Poaceae)竹亚科(Bambusoideae)多年生常绿植物,是一种大众化的药、食两用植物。 Bamboo is a grass family (Poaceae) Bambusoideae (Bambusoideae) perennial evergreen plant, is a popular drug, and edible plants. 竹子在东南亚广为分布,我国是世界竹子的中心产区之一,现有竹种300多种,竹林面积6000多万亩。 In Southeast Asia, bamboo is widely distributed, our country is one of the central areas of the world bamboo, the existing 300 kinds of bamboo, bamboo forest area of ​​over 6,000 acres. 竹子的医疗保健作用早已为我国劳动人民所认识,《本草求真》曰:“竹叶据书载,凉心缓脾、清痰止渴,为治上焦风邪烦热、咳逆喘促、呕哕吐血、一切中风惊痫等症,无非因其轻能解上、辛能散郁、甘能缓脾、凉能入心、寒能疗热故耳”。 Health effects of bamboo has long been recognized for our working people, "Materia Medica truth", saying: "According to the book carrying bamboo, cold heart slow spleen, phlegm thirst quencher for the treatment of coke wind cold Fanre, Keni breathlessness , vomiting hiccup vomiting, all embolism stroke, epilepsy, nothing can be light because the solution, can oct San Yu, Gan can slow spleen, cool to the heart, so cold to heat treatment ears. " 淡竹叶和淡竹沥就是中医一味传统的清热解毒药。 Fresh bamboo leaves and pale Xianzhuli is blindly traditional Chinese medicine drug detoxification. 竹子全身是宝,是当今世界最具有使用价值的植物之一。 Bamboo body is a treasure, is one of the world's plants have the most use value. 但在竹子的综合利用中,竹叶的研究和开发明显滞后,除极少量的药用和食用以外,基本上处于原始状态。 But in the comprehensive utilization of bamboo, bamboo research and development has lagged far behind, except for a very small amount of medicinal and edible, basically in pristine condition. 近年来,发明人对竹叶的有效成分及其生物活性进行了系统的研究,发现竹叶中含有丰富的黄酮等酚性化合物、蒽醌类化合物、萜类内酯、免疫活性多糖、特种氨基酸(δ-羟基赖氨酸)和锰、锌、铁、硒等微量元素,其中黄酮类化合物是其主要的功能成分。 In recent years, the inventors of the active ingredient of bamboo leaves and biological activity of a systematic study, it was found bamboo leaves are rich in flavonoids and other phenolic compounds, anthraquinone compounds, terpene lactones, immunoactive, special amino acids (delta-hydroxylysine), and manganese, zinc, iron, selenium and other trace elements, wherein the flavonoids are the main functional components. 竹叶所含的黄酮类化合物基本上皆是黄酮糖苷,相当部分是碳苷黄酮,如荭草苷木糖苷、异荭草苷、4'-甲氧基-牡荆苷等。 Flavonoids are substantially contained in bamboo flavone glycosides, a considerable portion of flavonoid C-glycosides such as orientin xylosidase, homoorientin, 4'-methoxy - vitexin like.

研究表明,竹叶提取物(Ebl)具有优良的抗自由基、抗氧化和抗衰老活性。 Studies have shown that ble (Ebl) has excellent anti-free radical, anti-oxidation and anti-aging activity. 大量的研究表明,Ebl具有很强的清除活性氧自由基和阻断亚硝化反应的能力;能显著诱导老龄小鼠内源性抗氧化酶系(SOD和GSH-Px)的活力、抑制脂质过氧化和清除过氧化产物;能显著增强小鼠的抗疲劳和抗应激能力;Ebl能显著降低大鼠血甘油三酯和总胆固醇的浓度,增加HDL-胆固醇浓度、降低LDL-胆固醇浓度,抑制大鼠体重的增加。 A number of studies, has strong Ebl Antioxygenation and ability to block the nitrosation reaction; significantly induce the activity of endogenous antioxidant enzymes in aged mice (SOD and GSH-Px), and inhibition of lipid Clear peroxide and peroxide product; significantly enhance the anti-stress and anti-fatigue mice; Ebl significantly reduce the concentration in rat serum triglyceride and total cholesterol, HDL- cholesterol concentration increase, decrease LDL- cholesterol concentration, inhibiting increase in body weight of rats. 动物试验的结果表明,Ebl降低血脂与诱导内源性SOD活力的作用与银杏叶提取物(Egb)相同,抑制脂质过氧化和诱导GSH-Px活力的作用明显优于Egb。 Animal studies showed that, Ebl lipid lowering effect and induce endogenous SOD activity and Ginkgo biloba extract (Egb) the same, inhibiting lipid peroxidation and GSH-Px activity inducing action was superior to Egb. 此外,尚未从竹叶中检出有害成分或抗营养因子。 Further, yet harmful components from the detection or anti-nutritional factors in bamboo leaves. 急性毒性试验表明,Ebl经口的LD50>10g/kg体重,可视为实际无毒;Ames试验表明,Ebl无致突变性。 Acute toxicity tests showed, Ebl oral LD50> 10g / kg body weight, may be considered as nontoxic; Ames test showed that, Ebl no mutagenicity. 所以说,Ebl是一种安全高效的生物黄酮保健因子,可广泛应用于功能性食品和多种老年退行性疾病的防治。 So, Ebl is a safe and effective bioflavonoids health factors, can be widely used in the prevention and treatment of functional foods and a variety of degenerative diseases.

本发明的目的在于利用我国资源十分丰富、且尚未得到有效开发利用的竹叶,提供一种工艺简便、提取率高,过程易控制、无污染、适合于大规模工业化生产的从竹叶中提取黄酮类化合物浸膏或粉剂的生产方法。 The purpose of the invention is the use of our country is very rich in resources and has not been effective development and utilization of bamboo, to provide a simple process, the extraction rate, the process is easy to control, pollution-free, suitable for large-scale industrial production of extracts from bamboo leaves production of flavonoids in the extract or powder.

从竹叶中提取黄酮类化合物浸膏或粉剂的生产方法,其特征是:以竹叶为原料,将竹叶洗净、烘干、破碎,用较低浓度(20-40%,体积百分浓度)的低级醇(甲醇或乙醇)为溶剂,以1∶10-15的固液比(重量百分比)热回流提取1-2小时,提取液浓缩后物理去杂,再用中级醇(丁醇或戊醇)液-液萃取,萃取液减压浓缩回收溶剂后,加少量水转溶,成黄酮类化合物浸膏,或再经真空或喷雾干燥,即制得黄酮类化合物粉剂。 Extraction production of flavonoids from the extract powder or bamboo, which is characterized in that: the bamboo as a raw material, the bamboo cleaning, drying, crushing, with low concentrations (20-40%, the volume percentage concentration) in a lower alcohol (methanol or ethanol) as the solvent, the solid-liquid extraction of 1-2 hours 1:10-15 ratio (% by weight) was heated under reflux, the extract was concentrated to physical heteroaryl, then intermediate alcohol (butanol or pentanol) liquid - liquid extraction, the extract was concentrated under reduced pressure to recover the solvent, redissolution small amount of water, to extract flavonoids, or additionally by vacuum or spray drying, i.e. flavonoids obtain powders.

本发明是这样实现的:竹叶→洗净→烘干→破碎→乙醇~水溶液热回流提取→过滤→减压浓缩→静止絮凝→分离去杂→正丁醇分步萃取→减压浓缩回收溶剂→热水转溶→减压浓缩→(浸膏)→真空或喷雾干燥→竹叶黄酮粉剂该发明的技术要点是根据竹叶黄酮类化合物的组成特点和理化特性(良好的亲水性和热稳定性)、采用乙醇水溶液一次性热回流提取;根据杂质在一定条件下的絮凝和沉淀特性,用物理方法分离去杂;然后用正丁醇分步萃取,回收溶剂,经水转溶后得浸膏,再干燥即得粉剂。 The present invention is achieved: bamboo → washing → drying → crushing → ~ hot aqueous ethanol reflux extraction concentrated under reduced pressure → → → filtration → static flocculation separation undoping → → butanol extraction step to recover the solvent concentrated under reduced pressure hot concentrated → → → redissolution (extract) → → vacuum or spray drying powders EOB technical points of the invention is reduced depending on the composition and characteristics of physicochemical properties of bamboo flavonoids (hydrophilicity and good heat stability), using the one hot aqueous ethanol reflux extraction; the flocculation and precipitation of impurities characteristic under certain conditions, to physically separating heteroaryl; step then extracted with butanol, recovering the solvent, redissolved with water to give the extract, and then dried to obtain a powder. 工艺简捷,易于控制;设备投入少,分离效果好,提取率高;无有机溶剂残留,成品质量好;整个过程不使用有毒、有害试剂和强酸强碱,基本上无“三废”排放,不构成环境污染。 Simple process, easy to control; less equipment investment, separation, and extraction rate; no residual organic solvents, good product quality; the whole process does not use toxic and hazardous reagents, and acid and alkali substantially free "waste" discharge, does not constitute environmental pollution. 本发明工艺适用于连续大批量生产。 The process of the invention is suitable for continuous mass production.

本发明使长期废弃的竹叶得到了深度开发,既实现了竹子的整株利用,由为社会提供了一种来源广阔、优质廉价的生物黄酮新资源,对人类的衰老、肿瘤和心血管病等退型性疾病的预防和治疗具有重要意义。 The present invention enables long-abandoned bamboo leaves get in-depth development, only to achieve the whole use of bamboo by providing a source for the broad community, bioflavonoids new high-quality low-cost resources, human aging, cancer and cardiovascular disease prevention and treatment of diseases such as type of retirement is important.

竹叶总黄酮制备的工艺要点如下:(1)竹叶原料可以是散生竹或丛生竹的干青叶,以刚竹属的种类(如毛金竹、桂竹、毛竹、哺鸡竹、早园竹、高节竹、紫竹、斑竹等)为佳,理想的采收期是秋冬季(9~12月)。 Process points total EOB prepared as follows: (1) bamboo leaf stem material can be scattered or clumping bamboo, Phyllostachys to type (such as wool bamboo, Makino, bamboo, bamboo feeding chicken, praecox bamboo, high section of bamboo, black bamboo, owner, etc.) is better, it is the ideal harvest time in autumn and winter (September to December).

(2)一次性热回流提取。 (2) a disposable thermal reflux extraction. 取干青叶的碎片,用1∶10~15料液比、20~40%浓度的乙醇溶液)热回流提取1~2hr,周期短、设备占用率低、提取较完全。 Dry pieces of leaf taken, by solid-liquid ratio of 1:10 to 15, 20 to 40% ethanol solution concentration) thermal reflux extraction 1 ~ 2hr, short cycle, equipment utilization is low, more complete extraction. 鉴于竹叶原料的广泛性和低廉的价格,该一次性提取法是最经济、合理的方法。 Given the breadth and low prices of raw bamboo leaves, the one-time extraction method is the most economical and reasonable method.

(3)物理法去杂。 (3) physical methods dedoping. 竹叶提取液浓缩到1∶1体积左右(即1kg干叶得1kg左右的浓缩液),在自然降温的静止过程中,内含的大分子杂质和部分多酚类成分发生絮凝,自然沉降,用离心分离、压滤或真空抽滤的方法除去,具有良好的澄清效果。 Bamboo extract was concentrated to a volume of about 1:1 (i.e. 1kg dried leaves obtained concentrate was approximately 1kg), still in the process of natural cooling, and the portion containing impurities macromolecules polyphenolic component flocculation, sedimentation Nature, by centrifugation, vacuum filtration, or pressure filtration method for removing, having a good refining effect. 该物理去杂法较之常规的醇沉或其他化学方法澄清,大大节省了生产成本,并且避免了化学试剂对提取物质量可能带来的不良影响。 The physical undoping process than conventional alcohol precipitation, or other chemical clarification methods, saving the cost of production, and avoids the adverse effects of chemical agents may bring the quality of the extract.

(4)竹叶黄酮的精制采用正丁醇液~液萃取法。 (4) purification using EOB butanol solution - liquid extraction. 由于正丁醇对竹叶黄酮糖甙有很好的溶解性和选择性,加之其沸点较高、在室温下不易挥发,比重小、易于分离和回收,且无毒、无害。 Because butanol solubility and good selectivity for bamboo flavone glycosides, together with its higher boiling point, less volatile at room temperature, the proportion of small, easy separation and recovery, and non-toxic, harmless. 用正丁醇进行有效次数的萃取,黄酮提取率高,成品色泽金黄。 It was extracted with n-butanol number of effective, high flavonoid, finished golden color.

(5)正丁醇萃取液经过减压浓缩、回收溶剂后,提取物再用少量的热水转溶成黄酮类化合物浸膏,再经真空干燥或喷雾干燥即得黄酮类化合物粉剂。 (5) n-butanol extract was concentrated under reduced pressure, the solvent is recovered, then a small amount of hot water extracts were redissolved into flavonoid extract, and then to obtain the dried powders of flavonoids in vacuo or spray drying.

用本发明的技术制备的竹叶黄酮类化合物粉剂为黄色粉末或黄色结晶颗粒,带有明显的竹叶芳香,总黄酮含量在24~30%,绝大部分是黄酮糖苷,其中含相当数量的碳苷黄酮,如荭草苷木糖苷、异荭草苷和4'-甲氧基-牡荆苷等。 Flavonoids in bamboo powder prepared by the inventive technique as a yellow crystalline powder or yellow particles, with a clear aromatic bamboo, total flavonoid content 24 to 30%, most of the flavone glycosides, which contain a significant amount of flavonoid C-glycosides such as orientin xylosidase, homoorientin and 4'-methoxy - vitexin like. 其他成分还有植物酚酸、蒽醌、萜类内酯、含氮化合物、可溶性糖和矿物质等。 There are other components of the plant phenolic, anthraquinone, terpene lactones, nitrogen-containing compounds, soluble sugars and minerals.

实施例1:毛金竹(Phyllostachy nigra var.henonis(Bean)Stapf exRendle)的干青叶1kg,用破碎机破碎成10目左右的粗片后放入20L的提取罐中,加入30%的乙醇12kg,浸泡1hr,加热回流1.5hr。 Example 1: Mao bamboo (Phyllostachy nigra var.henonis (Bean) Stapf exRendle) of the leaf stem 1kg, with a crusher into coarse mesh sheet into about 10 in an extraction tank 20L, 30% ethanol 12kg soak 1hr, heated to reflux for 1.5hr. 粗提液(约10kg)经过滤后入真空浓缩器中减压浓缩(同时回收溶剂),浓缩至体积为1L左右时出料,静止8hr,真空抽滤去除杂质,清液用同体积的正丁醇萃取三次(正丁醇预先用水饱和,40~50℃保温萃取、每次搅拌0.5~1hr),合并正丁醇相入真空浓缩器中减压浓缩回收溶剂,提取物用热水转溶,减压浓缩至一定浓度后成浸膏,或再经喷雾干燥得25g黄色粉末,其总黄酮含量为28%实施例2:2kg桂竹(Phyllostachy bambusoides Sieb.Et Zucc)的干青叶,破碎成10目左右的粗片放入50L的提取罐中,加入35%的乙醇30kg,浸泡0.5hr,加热回流1hr,提取液过滤后放入储罐中,用真空浓缩器分批浓缩至最终体积约为2L,静止6hr,离心分离去除杂质,清液用2倍体积的正丁醇萃取2次,合并正丁醇相,减压浓缩回收溶剂,提取物经热水转溶后,减压浓缩至比重大于1.12后成浸膏,或再经真空干燥 The crude extract (about 10kg) was filtered into the vacuum concentrator and concentrated under reduced pressure (while recovering the solvent), concentrated to a volume around the discharge when 1L, 8hr stationary, vacuum filtration removal of impurities, the same volume of supernatant with n and extracted three times with butanol (n-butanol saturated with water beforehand, 40 ~ 50 ℃ heat extraction, each time stirring 0.5 ~ 1hr), the combined butanol phase was concentrated under vacuum to recover the solvent was concentrated at reduced pressure, redissolved in hot water extract , and concentrated under reduced pressure to a certain concentration to the extract, or spray drying and then 25g of a yellow powder having a total flavone content of 28% Example 2: 2kg wallflower (Phyllostachy bambusoides Sieb.Et Zucc) of the leaf stem, broken into about 10 mesh coarse pieces into the extraction tank 50L, 35% ethanol was added 30kg, soaking 0.5hr, heated to reflux for 1 hr, the extract was filtered into a tank, concentrated using a vacuum concentrator to a final batch volume of about after as 2L, still 6 hr, centrifuged to remove impurities, the supernatant with 2 volumes of n-butanol extracted twice, n-butanol phases were combined and concentrated under reduced pressure to recover the solvent, redissolution hot water extract was concentrated under reduced pressure to after a specific gravity greater than 1.12 into extract, and then dried under vacuum, or 得45g黄色粉末,总黄酮含量为27.6%。 To give 45g of a yellow powder, 27.6% of total flavonoids.

Claims (1)

1.从竹叶中提取黄酮类化合物浸膏或粉剂的生产方法,其特征是:以竹叶为原料,将竹叶洗净、烘干、破碎,用20~40%体积百分浓度的甲醇或乙醇为溶剂,以1∶10~15的固液重量百分比热回流提取1~2小时,提取液浓缩后物理去杂,再用丁醇或戊醇液~液萃取,萃取液减压浓缩回收溶剂后,加少量水转溶,成黄酮类化合物浸膏,或再经真空或喷雾干燥,即制得黄酮类化合物粉剂。 1. Production method of extracting flavonoids from the extract or powder of bamboo, which is characterized in that: the bamboo as a raw material, the bamboo cleaning, drying, crushing, with a concentration of 20 percent by volume to 40% methanol or ethanol as solvent extraction 1 to 2 hours in a solid-liquid heated to reflux 1:10 weight percent to 15, the extract was concentrated to physical heteroaryl, then - liquid extraction butanol or pentanol extract was concentrated under reduced pressure recovering the solvent, redissolution small amount of water, to extract flavonoids, or additionally by vacuum or spray drying, i.e. flavonoids obtain powders.
CN 98104564 1998-03-17 1998-03-17 Productive method for extracting flavonoid compound extract or powder from bamboo leaf CN1066055C (en)

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