CN103070289B - Natural product feed additive from bamboo - Google Patents

Natural product feed additive from bamboo Download PDF

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CN103070289B
CN103070289B CN201310013756.5A CN201310013756A CN103070289B CN 103070289 B CN103070289 B CN 103070289B CN 201310013756 A CN201310013756 A CN 201310013756A CN 103070289 B CN103070289 B CN 103070289B
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bamboo
extract
vacuum
content
condition
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CN103070289A (en
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张英
黄骆镰
吴晓琴
李栋
姜文进
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Zhejiang University ZJU
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Zhejiang University ZJU
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Abstract

The invention discloses a natural product feed additive from a bamboo, which comprises the following components in part by weight: 1-4 parts of bamboo leaf extract, 1-2 parts of bamboo extract and 1-2 parts of bamboo shaving extract or is prepared by adopting the following steps: mixing the 1-4 parts of bamboo leaf extract, the 1-2 parts of bamboo extract and the 1-2 parts of bamboo shaving extract according to the dry mass ratio to obtain a bamboo mixture; and extracting the bamboo mixture, wherein an obtained extract is the feed additive. The feed additive is a yellow, brown or light yellow power or particle preparation, has the bamboo fragrance and has a total phenolic content of 8-30 percent, a polysaccharide content of 5-15 percent and a triterpene content of 3-10 percent. The feed additive has multiple functions of an antioxidant, a nutrition enhancer, an immunopotentiator, a lipid metabolism regulator, an animal product quality improver, a flavoring agent, a preservative, a feed attractant and the like.

Description

Derive from the natural goods feed addictive of bamboo
Technical field
The present invention relates to a kind of natural goods feed addictive that derives from bamboo, belong to animal feed and cultural technique field.
Background technology
The feed industry of China is started in 19 century 70s, has exceeded 1.5 hundred million tons to forage yield in 2011.Along with China poultry, domestic animal, the raising of culture fishery intensive degree and taking place frequently of epidemic disease, chemicals taking antibiotic, synthetic drug and steroid hormone as representative generally uses in feedstuff industry, though improved to a certain extent the production efficiency of aquaculture, but many negative effects are also brought, cultivating object as made develops immunity to drugs, and causing its Immune Dysfunction, edible quality significantly declines.Meanwhile, the residual human health that threatens of a large amount of antibiotic, hormone and chemicals in product, causes the worry to animal production safety in global range.Along with the theory of people's " back to nature ", " natural environmental-protective ", " green consumption " gradually becomes the general trend of events, the cry of, safety natural to developing, nutrition, multi-functional feed addictive is more and more higher, and market also constantly expands.
China have the title of " bamboo kingdom ", has very abundant bamboo resource, and Bamboo products has long edible and medicinal history in China and even Asia.Inventor's bamboo active ingredient that begins one's study from the beginning of the nineties in last century, from bamboo different parts, research and develop " bamboo the is got thing " series taking instant bamboo leaf powder, bamboo-leaves flavones, AOB, caulis bambusae in taenian triterpene, bamboo shoots sterol and bamboo shoots amino acid peptide as representative, be used widely in fields such as food, drink, health (functional) food, natural drug and food additives and cosmetic additive agents.Nearly ten years, the scientific research to bamboo chemistry element and product development have caused the extensive attention of industry, and all kinds of health-oriented products that contain bamboo active ingredient continue to bring out.
A large amount of researchs show, bamboo extractive is taking polyphenols as main, its characteristic compound comprises taking orientoside, Lutonaretin, Vitexina, Saponaretin as the carbon glycosides flavones of representative and taking the plant phenolic acid as representative to fragrant acid, chlorogenic acid, caffeic acid, forulic acid, there is significant biological antioxidant activity and radical scavenging activity, can effectively regulate lipid-metabolism and the energetic supersession of body, there is broad-spectrum antiseptic, bacteriostatic activity, simultaneously with typical leaf of bamboo delicate fragrance; Bamboo sprout extract mainly, containing polysaccharide, oligopeptides, amino acid, mineral matter and trace element, has significant immunologic enhancement and quality-improving effect, and the while is with the fresh fragrant flavour of typical bamboo shoots; Caulis Bambusae In Taenia extract is mainly taking triterpene and polysaccharose substance as main, and its representational pentacyclic triterpenoid is suberone and lupenone, have significant antifatigue, anti-stress and active anticancer.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of safe, economic, natural goods feed addictive of effectively deriving from bamboo, and this natural goods feed addictive has the functions such as nutrition fortifier concurrently.
In order to solve the problems of the technologies described above, the invention provides a kind of natural goods feed addictive that derives from bamboo:
One-tenth by following mass parts is grouped into: 1～2 part of 1～4 part of bamboo extractive, 1～2 part of bamboo sprout extract and Caulis Bambusae In Taenia extract; Or be prepared as follows and obtain:
By after 1～4 part, the leaf of bamboo, 1～2 part, bamboo shoots, 1～2 part of mixing of caulis bambusae in taenian, obtain bamboo mixture according to butt mass ratio; Bamboo mixture is extracted, and the extract of gained is feed addictive.
Improvement as the natural goods feed addictive that derives from bamboo of the present invention: this feed addictive is yellow, brown color or lurid powder or granular preparation, with bamboo fragrance, total phenol content 8%～30 %, polyoses content 5 %～15 %, triterpene content is 3 %～10 %; Above-mentioned % is quality %.
Further improvement as the natural goods feed addictive that derives from bamboo of the present invention: bamboo is mao bamboon or phyllostachys nigra var. henonis.
Remarks explanation: bamboo of the present invention, refer to the kind of grass family, Bambusoideae, Phyllostachys, preferably mao bamboon (another name bamboo, Meng Zongzhu, Miao Zhu, cat bamboo etc.) Phyllostachys heterocycla var.pubescens (Mazel) Ohwi and phyllostachys nigra var. henonis (another name: golden mao bamboon, henon bamboo etc.) Phyllostachys nigra var. hnonis (Bean) Stepf ex Rendle.
Further improvement as the natural goods feed addictive that derives from bamboo of the present invention:
Bamboo extractive is using water as the bamboo extractive that extracts solvent acquisition,
Bamboo sprout extract is using water as the bamboo sprout extract that extracts solvent acquisition,
Caulis Bambusae In Taenia extract is using alcohol (methyl alcohol or ethanol all can) as the Caulis Bambusae In Taenia extract that extracts solvent and obtain;
Bamboo mixture adopts following technique to extract:
Be preferably 30% taking volumetric concentration as 28 ~ 32%() ethanol water be extractant, extracting mode is hot reflux extraction or countercurrent leaching, the extract of gained is feed addictive.
Further improvement as the natural goods feed addictive that derives from bamboo of the present invention: bamboo mixture, in the time extracting with hot reflux extraction or countercurrent leaching mode, adopts microwave or ultrasonic wave to carry out assisted extraction.
Further improvement as the natural goods feed addictive that derives from bamboo of the present invention: be the full bamboo shoot of bamboo as the bamboo shoots of raw material, the exocuticle that is bamboo as the caulis bambusae in taenian of raw material; Be preferably: being at least one in bamboo shoot shell, bamboo shoot head and bamboo shoot liquid as the bamboo shoots of raw material, is at least one in bamboo powder and bamboo shaving as the caulis bambusae in taenian of raw material.
, the bamboo different parts as raw material of the present invention, is specifically described below:
Bamboo shoots as raw material both can adopt full bamboo shoot, were more preferably accessory substance---bamboo shoot shell, bamboo shoot head and the bamboo shoot liquid that adopts bamboo shoot processing; Referring to the exocuticle of bamboo as the caulis bambusae in taenian of raw material, is more preferably the accessory substance that adopts bamboo processing, leftover bits and pieces---bamboo powder or bamboo shaving when bamboo wood is scraped blue or green processing.
Further improvement as the natural goods feed addictive that derives from bamboo of the present invention: the one-tenth by following mass parts is grouped into: each 1 part of bamboo extractive, bamboo sprout extract and Caulis Bambusae In Taenia extract; This feed addictive is for broiler breeding, thus the quality of raising broiler chicken.
Further improvement as the natural goods feed addictive that derives from bamboo of the present invention: the one-tenth by following mass parts is grouped into: 2 parts of bamboo extractive 1,1 part of bamboo sprout extract and Caulis Bambusae In Taenia extracts; This feed addictive is for breeding layer chicken, thus the quality of raising egg.
Further improvement as the natural goods feed addictive that derives from bamboo of the present invention: the one-tenth by following mass parts is grouped into: 1 part of 1 part of bamboo extractive, 2 parts of bamboo sprout extracts and Caulis Bambusae In Taenia extract; This feed addictive is for yellow croaker cultivation, thus the quality of raising yellow croaker.
In the present invention:
Bamboo extractive, bamboo sprout extract, Caulis Bambusae In Taenia extract all can obtain by commercial form; Also can be prepared by the method for having published:
Bamboo extractive can be according to Chinese invention patent " Zhang Ying, from the leaf of bamboo, extract the production method of flavonoid compound extract or pulvis " (ZL 98 104564.2) be prepared, bamboo sprout extract can be according to Chinese invention patent " Zhang Ying etc., bamboo shoots amino acid peptide based composition and its production and use " (ZL 200510025443.7) be prepared, Caulis Bambusae In Taenia extract can be prepared according to Chinese invention patent " Zhang Ying etc., composition, method for making and the purposes of the total triterpenoid saponin unit extracting from bamboo " (ZL 02154401.8).
Particularly: the leaf of bamboo and bamboo shoots are using water as extracting solvent, and caulis bambusae in taenian adopts alcohol extracting (methyl alcohol or ethanol all can); In the time that bamboo different parts mixes extraction, adopt 30% ethanol-water solution extraction.Extraction process, taking hot reflux extraction and countercurrent leaching as main, can adopt microwave or ultrasonic wave assisted extraction simultaneously.
The present invention is according to the nutritional characteristic of different breeding object and metabolic characteristics, to be derived from active ingredient (leaf of bamboo polyphenol, bamboo shoots polysaccharide and the caulis bambusae in taenian triterpene of bamboo different parts (leaf of bamboo, bamboo shoots, caulis bambusae in taenian), be bamboo extractive, bamboo sprout extract and Caulis Bambusae In Taenia extract) carry out organic assembling, obtain the functional feed additive series of different proportionings.Feed addictive of the present invention is yellow, brown color or lurid powder or granular preparation, with bamboo fragrance, total phenol content 8%～30 %, polyoses content 5 %～15 %, triterpene content is 3 %～10 %, be a kind of good natural, immunopotentiating agent, fat metabolism regulators and quality improver, and there is certain attractant.
Feed addictive of the present invention has the several functions such as antioxidant, nutrition fortifier, immunopotentiator, fat metabolism regulators, animal product quality improver, flavoring agent, anticorrisive agent, phagostimulant concurrently, addition in feed is generally 0.1% ~ 0.6%(weight ratio), can be widely used in the propagating artificially of high-quality domestic animal, poultry and fish.Meanwhile, also help the oxidation that prevents high lipid food, extend the shelf life of feed.The present invention has advantages of useful human health, environmental protection and sustainable development simultaneously.
In sum, the present invention is based on studying for a long period of time and developing accumulated rich experiences bamboo chemistry element, according to nutritional characteristic and the metabolic characteristics of different breeding object, the extract that bamboo different parts is obtained carries out organic assembling and compatibility research, a kind of natural, safety, nutrition, multi-functional feed addictive are obtained, be applied in animal perfect compound feed, have the several functions such as antioxidant, nutrition fortifier, immunopotentiator, fat metabolism regulators, animal product quality improver, flavoring agent, anticorrisive agent, phagostimulant concurrently.Meanwhile, can, according to the specific demand of different breeding object, the active component proportioning in product be aligned.
Detailed description of the invention
Further illustrate content of the present invention below in conjunction with example, its object is to be convenient for people to understand better content of the present invention, but not limits the scope of the invention.
In addition, relating to the problem of several general character in embodiment is described below.
The assay method of 1 total phenol
1.1 principle
Forint (Folin) reagent is extremely unstable under alkali condition, can make phenolic compound reduce and be blue.Being that within the scope of 0.5～16 μ g/mL, the relation character of its concentration and absorbance closes Beer law in gallic acid concentration, can gallic acid be the total phenol content that sample is carried out quantitative colorimetric estimation and obtains in contrast.
1.2 Specification Curve of Increasing
Precision takes gallic acid and contrasts 10.0 mg in 100 mL measuring bottles, and 30 % dissolve with ethanol solution are also diluted to scale, obtain standard liquid; Accurate standard liquid 0,0.05,0.10,0.20,0.30,0.40,0.50 mL that draws is in 25 mL tool plug test tubes, and water pure water is diluted to 6.0 mL respectively, respectively adds Folin reagent 0.5 mL and 20 % Na in 0.5～8.0 min 2cO 3the aqueous solution 1.5 mL, are diluted with water to 10 mL, and test tube heats 2 h in 40 DEG C of water-baths, and cooling (ice bath) rapidly, with the cuvette of 1 cm, measured absorbance at the wavelength place of 765 nm.Taking trap as ordinate, concentration is abscissa drawing standard curve.
1.3 sample determination
Get appropriate sample dissolution in methanol solution, after filtration, be settled to 100 mL.Get liquid 0.50 mL to be measured in 25 mL tool plug test tubes, undertaken by " Specification Curve of Increasing " method, under 765nm wavelength, measure absorbance.
1.4 results are calculated
According to calibration curve, obtain the content to gallic acid that is equivalent to sample absorbance, obtain sample total phenol content by following formula:
Total phenol content (%)=(m 1× V 2)/(m × V 1) × 100
In formula:
M 1---establishing criteria opisometer calculates phenols content (mg) in test solution;
M---sample volume (mg);
V 1---liquid to be measured divides the volume of getting (mL);
V 2---the cumulative volume (mL) of liquid to be measured.
The assay method of 2 polysaccharide
2.1 principle
Adopt phenolsulfuric acid method to measure.Molecular weight is greater than 10000 daltonian polysaccharide after 80 % ethanol precipitations, be first hydrolyzed into monose, and dehydration generates alditol derivative rapidly, then generates orange-yellow compound with phenol under the effect of sulfuric acid, then with colorimetric method for determining.
2.2 Specification Curve of Increasing
Precision takes 25.0 mg glucose, dissolves and is settled to 100 mL with distilled water.Pipette above-mentioned glucose solution 0.1,0.2,0.4,0.6,0.8,1.0 mL in 25 mL colorimetric cylinders, all mend to 2.0 mL with pure water, shake up, add phenol reagent 1.0 mL, concentrated sulfuric acid reagent 5.0 mL, shake up immediately, cooling 30 min.Add phenol reagent 1.0 mL and concentrated sulfuric acid reagent 5.0 mL work blank with 2.0 mL pure water simultaneously.Measure absorbance at 490 nm wavelength places.
2.3 sample determination
Get in the ethanolic solution that 1～2 g sample is dissolved in 100 mL 80 %, put into refrigerator hold over night.Centrifugal 10 min of 4000 rpm, abandoning supernatant, residue uses pure water constant volume in 50 mL volumetric flasks.Get liquid 0.1～0.5 mL to be measured and replace standard liquid in 25 mL colorimetric cylinders, by " Specification Curve of Increasing " method, measure the absorbance at 490 nm wavelength places.
2.4 results are calculated
According to calibration curve, obtain the content of the glucose that is equivalent to sample absorbance, obtain the polyoses content of sample by following formula:
Polyoses content (%)=(m 1× V 2)/(m × V 1) × 100
In formula:
M 1---establishing criteria opisometer calculates glucose content in test solution (mg);
M---sample volume (mg);
V 1---liquid to be measured divides the volume of getting (mL);
V 2---the cumulative volume (mL) of liquid to be measured.
The assay method of 3 triterpenes
3.1 principle
Adopt vanillic aldehyde-glacial acetic acid, perchloric acid development process, colour developing principle is mainly the carboxyl dehydration that makes triterpene compound, form double bond structure, then generate polyenoid system through the reaction such as double bond migration, bimolecular condensation, and under acid effect, form cationic salts and develop the color.
3.2 Specification Curve of Increasing
Precision takes ginsenoside standard items 10.0 mg in 100 mL measuring bottles, adds anhydrous alcohol solution and is diluted to scale.Therefrom draw standard solution 0,0.1,0.2,0.4,0.6,0.8,1.0 mL, under heated condition, dry up solvent with nitrogen, add respectively 5 % vanillic aldehyde-glacial acetic acid 0.5 mL, then add perchloric acid 0.8 mL, after mixing in 65 DEG C of water-bath 15 min, take out in placement frozen water and be cooled to rapidly after room temperature, add glacial acetic acid 5 mL, vibration mixes; Taking zero pipe as blank, with the cuvette of 1 cm at 560 nm(panaxosides) locate to measure absorbance, taking the micrograms of panaxoside as abscissa, absorbance are as ordinate drawing standard curve.
3.3 sample determination
Get 1～2 g sample in 200 mL round-bottomed flasks, add absolute ethyl alcohol 100 mL, extract 2 h(in 80 DEG C of stirred in water bath and can be aided with ultrasonic wave processing), filter to get filtrate while hot and be settled to 250 mL.Get liquid 0.5～1.0 mL to be measured and replace standard liquid in 25 mL colorimetric cylinders, by " Specification Curve of Increasing " method, measure 560 nm places and measure absorbance.
3.4 results are calculated
According to calibration curve, obtain the content of ginsenoside that is equivalent to sample absorbance, obtain sample total triterpene contents by following formula, unit is that (g/100 is g) for gram every hectogram
Triterpene content=(m × V)/(V 1× M) × 10 -6× 100
In formula:
M---establishing criteria opisometer calculates ginsenoside amount in reactant liquor, and (μ g);
V---extract cumulative volume (mL);
V 1---the volume (mL) that liquid to be measured pipettes;
M---sample volume (g).
Embodiment 1, a kind of feed addictive that derives from bamboo, carry out following steps successively:
1) get the dry leafiness of mao bamboon (moisture content≤14 %) that autumn and winter season gathers and be crushed to 10 about orders, every 50 kg add pure water 500 L hot refluxs to extract 1.5 h, after filtration by the filtrate Vacuum Concentration of gained (vacuum 0.09 Mpa, temperature≤55 DEG C) to solid content be 25 %(quality %) left and right, spraying dry (185 DEG C of EATs, 90 DEG C of leaving air temps), obtains bamboo extractive 4.1 kg(brown color powder, total phenol content 29.02 %, polyoses content 8.21 %).
2) get boiled bamboo shoots can processing accessory substance--a bamboo shoot shell and bamboo shoot dry powder (30 order left and right, moisture content≤12 %, the dry weight of bamboo shoot shell and bamboo shoot head is than being 1:1.5), every 50 kg add pure water 500 L hot refluxs to extract 2 h, after filtration by the filtrate Vacuum Concentration of gained to solid content 30 about %, vacuum drying (vacuum 0.09 Mpa, temperature≤65 DEG C), obtain bamboo sprout extract 5.3 kg (light brown powder contains polysaccharide 23.31 %, triterpene 2.30 %).
3) get the accessory substance of bamboo processing--after bamboo shaving is dry, (moisture content≤10 %) is crushed to 10-20 order, every 50 kg add edible alcohol (ethanol volumetric concentration is 93-94 %) 500 L normal temperature (10 ~ 25 DEG C) lixiviate 48 h, after filtration by the filtrate Vacuum Concentration of gained (vacuum 0.09 Mpa, temperature≤45 DEG C) reclaim solvent, (power density is 1w/g～2w/g to vacuum microwave drying; Vacuum is 0.08 Mpa; Be 2h～3h drying time), obtain Caulis Bambusae In Taenia extract 2.38 kg(shallow green powders, containing triterpene 11.76 %, total phenol 8.90 %).
Above bamboo extractive, bamboo sprout extract and Caulis Bambusae In Taenia extract are combined into following formula:
Formula 1 #: get the each 1 part of composition feed addictive of bamboo extractive, bamboo sprout extract and Caulis Bambusae In Taenia extract, in gained feed addictive, contain total phenol 12.31 %, polysaccharide 10.51 %, triterpene 4.69 %.
Formula 2 #: get 2 parts of bamboo extractive 1,1 part of bamboo sprout extract and Caulis Bambusae In Taenia extracts, in gained feed addictive, total phenol content is 11.46 %, and polyoses content is 7.88 %, and total triterpene contents is 6.46 %.
Formula 3 #: get the each 1 part of composition feed addictive of 2 parts of bamboo extractives, bamboo sprout extract and Caulis Bambusae In Taenia extract, in gained feed addictive, contain total phenol 16.24 %, polysaccharide 9.93 %, triterpene 3.52 %.
Formula 4 #: get each 0.5 part of 3 parts of bamboo extractives, bamboo sprout extract and Caulis Bambusae In Taenia extract, in gained feed addictive, total phenol content is 23.24 %, and polyoses content is 11.99 %, and total triterpene contents is 3.52 %.
Formula 5 #: get 1 part of bamboo extractive, 2 parts of bamboo sprout extracts and 1 part of composition feed addictive of Caulis Bambusae In Taenia extract, in gained feed addictive, contain total phenol 9.23 %, polysaccharide 13.71 %, triterpene 4.09 %.
Embodiment 2
According to butt mass ratio, get 3 parts of the dry leafiness (moisture content≤14 %) of phyllostachys nigra var. henonis, a bamboo shoot shell and bamboo shoot dry powder (moisture content≤12 %, the dry weight of bamboo shoot shell and bamboo shoot head is than being 1:1.5) 1 part, 2 parts and bamboo shaving powder (moisture content≤10 %) mix, every 50 kg add 30 % (v/v) ethanol-water solution of 500 L, 2 h are extracted in hot reflux, filtrate Vacuum Concentration to solid content in 30 about %, vacuum drying (vacuum 0.09 Mpa, temperature≤65 DEG C) after, obtain feed addictive 4.3 kg(brown color powder of the present invention, total phenol content 23.28 %, polyoses content 12.13 %, triterpene content is 6.24 %).Be denoted as formula 6 #.
Experiment 1
The present invention is by the feed addictive prescription in embodiment 11 #for high-quality broiler breeding.
1, experimental design
With stone qi beard chicken (Hong Kong modern agriculture Co., Ltd hybridization cultivate a kind of yellow-feather broiler) as subjects.Method is as follows: select the healthy chick of 360 26 ages in days, initial body weight is (375.12 ± 8.30) g, is divided at random 4 groups, every group of 3 repetitions, 30 of each repetitions.Control group fed does not add basal diet (basal diet is powdery compound feed, with reference to U.S. NRC(1994) the broiler chicken nutritional need preparation of this additive).The test group of basic, normal, high three dosage is added respectively the formula 1 of 0.15 %, 0.30 % and 0.60 % in daily ration #additive.Employing is raised in cages, and the culture experiment cycle is 49 days, sampling inspection during to 75 age in days.
2, result of the test
(1) immunologic enhancement
In daily ration, add the feed addictive of the present invention (formula 1 of 0.15 %～0.60% #), can significantly improve the content of thymus index, bursa of farbricius index, serum lysozyme level and Immunoglobulin in Serum IgA and the IgM of broiler chicken.Show that it has played significant immunologic enhancement, result is as shown in table 1.The raising of broiler chicken immunologic function can effectively reduce the use of antibiotic and other chemicalses.
The impact of table 1 on broiler chicken immune indexes
Note: colleague's data shoulder mark, different lowercase alphabets show significant difference (P < 0.05), identical or without not significantly (P > 0.05) of letter representation difference.Lower same.
(2) regulating lipid metabolism
In daily ration, add the formula 1 of 0.15 %～0.6 % #, can significantly reduce the content of abdomen fat rate, subcutaneous fat thickness, hepatic fat content and the serum TG of broiler chicken, the results are shown in Table 2.Show that in daily ration of broiler, adding feed addictive of the present invention has played significant functions of lowering blood-fat and reducing weight, is conducive to the lean meat species chicken of production high-quality.
The impact of table 2 on Broiler Chicken lipidosis
(3) improve meat quality
In daily ration, add the formula 1 of 0.15 %～0.6 % #, the pH value of brisket after can significantly improving fat content, adhesiveness, the elasticity of chest muscle and slaughtering 45 min, significantly reduces drip loss and the hardness of chicken, the results detailed in Table 3.Show that feed addictive of the present invention can significantly improve the edible quality of chicken.
The impact of table 3 on chicken meat quality index
(4) improve nutritive value
In daily ration, add the formula 1 of 0.15 %～0.6 % #significantly improve ratio and the total amino acid content of essential fatty acid in chicken, and the content of the alkali metal such as potassium, calcium, magnesium, iron, zinc, result is as shown in table 4.Show, feed addictive of the present invention can significantly improve the nutritive value of broiler chicken.
The impact of table 4 on chicken nutritive index
In order fully to prove creative place of the present invention, inventor has also carried out following contrast experiment:
Above-mentioned feed addictive is all set as to middle dosage (i.e. interpolation 0.30% in daily ration of broiler), and control group is still basal diet.Every group of sample number is 30, raises in cages.After off-test, randomly draw 10 for every group, get chicken blood, measure immunologic function; All the other are equal to experiment 1.
Comparative example 1-1 ~ comparative example 1-7:
By " feed addictive prescription 1 used in experiment 1 #" make respectively " feed addictive prescription 3 into #", " feed addictive prescription 4 #", " feed addictive prescription 5 #", " feed addictive prescription 6 #", detect according to above-mentioned contrast experiment, thereby obtain comparative example 1-1 ~ comparative example 1-4 respectively;
Get the each 1.5 parts of composition feed addictives of bamboo extractive and bamboo sprout extract, substitute " feed addictive prescription 1 used in experiment 1 with this feed addictive #", detect according to above-mentioned contrast experiment, thereby obtain comparative example 1-5 respectively;
Get the each 1.5 parts of composition feed addictives of bamboo extractive and Caulis Bambusae In Taenia extract, substitute " feed addictive prescription 1 used in experiment 1 with this feed addictive #", detect according to above-mentioned contrast experiment, thereby obtain comparative example 1-6 respectively;
Get the each 1.5 parts of composition feed addictives of bamboo sprout extract and Caulis Bambusae In Taenia extract, substitute " feed addictive prescription 1 used in experiment 1 with this feed addictive #", detect according to above-mentioned contrast experiment, thereby obtain comparative example 1-7 respectively.
The experimental result of gained is as follows:
When adding in daily ration of broiler after 0.30% feed addictive of the present invention, compared with control group, the immune indexes of each group all has enhancing in various degree, but all not as feed addictive prescription 1 #, result is as shown in table 5.
The impact of table 5 on broiler chicken immune indexes
Experiment 2
The present invention is by the feed addictive prescription in embodiment 12 #for high-quality breeding layer chicken.
1, experimental design
The yellow plumage laying hen of selecting 360 180 ages in days, is divided into 4 groups at random, every group of 3 repetitions, 30 of each repetitions.Control group fed does not add the basal diet of this additive, and test group is added the formula 2 of 0.60 % in basal diet #.Test period is 20 days.This test is carried out in surrounding area, Nanchang, and when off-test, the Countryside Egg sample that extracts local peasant household compares.
2, result of the test
Result is as shown in table 6.In daily ration, add the formula 2 of 0.6 % #feed laying hen after 20 days, every index of quality of determination test group and blank group egg respectively, show that feed addictive of the present invention can make egg size, eggshell strength, antalzyme activity, egg proportion, protein height, hangh unit enlarge markedly, show to add feed addictive of the present invention in daily ration and can significantly improve the quality of egg.
The impact of table 6 on egg quality
Experiment 3
The present invention is by the feed addictive prescription in embodiment 13 #, 4 #, 5 #with the formula 6 in embodiment 2 #for propagating artificially of high-quality large yellow croaker.
1, experimental design
Selecting average weight is that (150.06 ± 11.35 large yellow croaker fingerling 3600 tails g), are divided into 6 groups (every group of 3 repetitions, each repetition 200 tail large yellow croakers) at random.Control group adopts traditional cultural method chilled or small fish shrimp of feeding.The test group large yellow croaker full price expanded pellet feed (fish meal, shrimp crab class processing byproduct, fish oil, vitamin and mineral substance premix and soy lecithin oil) of feeding, adds respectively the formula 3 in the embodiment 1 of 0.30 % dosage in the formula of this feed #, 4 #, 5 #with the formula 6 in embodiment 2 #, establish a positive controls (adding 0.10 % astaxanthin in daily ration) simultaneously, respectively organize large yellow croaker feed ingredient and measure in table 7.The culture experiment cycle is 13 weeks (from early June to by the end of October), and monthly sampling should be carried out, and every group is extracted 10 samples and carry out analytical test.
2, result of the test
(1) immunologic enhancement
In feed, add the formula 3 of 0.30 % #, 4 #, 5 #with 6 #, can significantly improve leukocytes phagocytic index, antalzyme activity, the complement vigor of large yellow croaker serum, to fill a prescription 5 #effect be best, result is as shown in table 7.Show that feed addictive of the present invention has played significant immunologic enhancement, its effect is generally better than the positive control of astaxanthin.The raising of cultured large yellow croaker immunologic function contributes to reduce the use of antibiotic and other chemicalses.
The impact of table 7 on cultured large yellow croaker immune indexes
(2) reduce fat deposition
In feed, add the formula 3 of 0.30 % #, 4 #, 5 #, 6 #can significantly reduce the fat content of Pseudosciaena crocea meat and liver, with to fill a prescription 5 #effect the most remarkable, result is as shown in table 8.Show that feed addictive of the present invention can effectively regulate the lipid-metabolism of large yellow croaker, reduce body fat deposition, the liver and gall syndrome of control cultured large yellow croaker, significantly promotes quality.
The impact of table 8 on large yellow croaker fat deposition
Index Control group Formula 3 #Group Formula 4 #Group Formula 5 #Group Formula 6 #Group Astaxanthin group
The flesh of fish/% 6.33±0.42 a 4.62±0.10 b 4.21±0.32 bc 3.79±0.17 c 4.79±0.36 b 3.63±0.48 c
Liver/% 15.31±0.89 a 9.67±0.40 b 8.97±0.23 bc 7.74±0.75 c 9.98±0.81 b 8.15±0.66 c
Comparative example 3-1 ~ comparative example 3-4,
Make " feed addictive " used in experiment 3 into " feed addictive prescription 1 #", all the other with experiment 3, thereby comparative example 3-1;
Get each 2 parts of bamboo extractive and bamboo sprout extract and form feed addictives, substitute " feed addictive " used in experiment 3 with this feed addictive, all the other same experiments 3, thus obtain respectively comparative example 3-2;
Get each 2 parts of bamboo extractive and Caulis Bambusae In Taenia extract and form feed addictives, substitute " feed addictive " used in experiment 3 with this feed addictive, all the other same experiments 3, thus obtain respectively comparative example 3-3;
Get each 2 parts of bamboo sprout extract and Caulis Bambusae In Taenia extract and form feed addictives, substitute " feed addictive " used in experiment 3 with this feed addictive, all the other same experiments 3, thus obtain respectively comparative example 3-4.
The experimental result of gained is as follows:
(1) immunologic enhancement
In compound fish bait used specially for pseudosciaena crocea, add 0.30% feed addictive prescription 1 #(comparative example 3-1) afterwards, compared with chilled control group, every immune indexes has certain enhancing.And obviously inferior (the comparative example 3-2 ~ 3-4) of effect of bamboo extractive, bamboo sprout extract and Caulis Bambusae In Taenia extract three combination of two compatibility, result is as shown in table 9.But all not as formula 5 #group.
The impact of table 9 on cultured large yellow croaker immune indexes
(2) reduce fat deposition
In compound fish bait used specially for pseudosciaena crocea, add 0.30% feed addictive prescription 1 of the present invention #(comparative example 3-1) afterwards, compared with control group, fat content in large yellow croaker dorsal muscles and liver has certain decline, and obviously inferior (the comparative example 3-2 ~ 3-4) of effect of bamboo extractive, bamboo sprout extract and Caulis Bambusae In Taenia extract three combination of two compatibility, result is as shown in table 10.But all not as formula 5 #group.
The impact of table 10 on large yellow croaker fat deposition
Index Chilled control group Comparative example 3-1 Comparative example 3-2 Comparative example 3-3 Comparative example 3-4
The flesh of fish/% 6.33±0.42 a 4.76±0.10 c 5.21±0.33 b 5.73±0.21 b 5.13±0.28 b
Liver/% 15.31±0.89 a 9.87±0.40 c 11.89±0.21 b 12.05±0.42 b 12.33±0.38 b
Finally, it is also to be noted that, what more than enumerate is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, can also have many distortion.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.

Claims (3)

1. the natural goods feed addictive that derives from bamboo, is characterized in that:
One-tenth by following mass parts is grouped into: each 1 part of bamboo extractive, bamboo sprout extract and Caulis Bambusae In Taenia extract; This feed addictive is for broiler breeding, thus the quality of raising broiler chicken;
The preparation method of bamboo extractive is:
The dry leafiness of mao bamboon of getting moisture content≤14% of autumn and winter season collection is crushed to 10 orders, every 50kg adds pure water 500L hot reflux to extract 1.5h, after filtration, be 25% by the filtrate of gained Vacuum Concentration to solid quality content under the condition of vacuum 0.09Mpa and temperature≤55 DEG C, under the condition of 90 DEG C of 185 DEG C of EATs and leaving air temps, spraying is dry, obtain bamboo extractive 4.1kg, for brown color powder, total phenol content 29.02%, polyoses content 8.21%;
The preparation method of bamboo sprout extract is:
Get boiled bamboo shoots can processing accessory substance--a bamboo shoot shell and bamboo shoot dry powder, every 50kg adds pure water 500L hot reflux to extract 2h, after filtration by the filtrate Vacuum Concentration of gained to solid content 30%, vacuum drying under the condition of vacuum 0.09Mpa and temperature≤65 DEG C, obtain bamboo sprout extract 5.3kg, for light brown powder, containing polysaccharide 23.31%, triterpene 2.30%;
Described bamboo shoot shell and a bamboo shoot dry powder are 30 orders, moisture content≤12%, and the dry weight of bamboo shoot shell and bamboo shoot head is than being 1:1.5;
The preparation method of Caulis Bambusae In Taenia extract is:
Get the accessory substance of bamboo processing--bamboo shaving is crushed to 10-20 order after being dried to moisture content≤10%, it is that the edible alcohol 500L of 93-94% is in the normal temperature lixiviate 48h of 10～25 DEG C that every 50kg adds ethanol volumetric concentration, after filtration, the filtrate of gained Vacuum Concentration under the condition of vacuum 0.09Mpa and temperature≤45 DEG C is reclaimed to solvent, be that 1w/g～2w/g, vacuum are vacuum microwave drying under 0.08Mpa and the drying time condition that is 2h～3h in power density, obtain Caulis Bambusae In Taenia extract 2.38kg, for shallow green powder, containing triterpene 11.76%, total phenol 8.90%.
2. the natural goods feed addictive that derives from bamboo, is characterized in that:
One-tenth by following mass parts is grouped into: 2 parts of bamboo extractive 1,1 part of bamboo sprout extract and Caulis Bambusae In Taenia extracts; This feed addictive is for breeding layer chicken, thus the quality of raising egg;
The preparation method of bamboo extractive is:
The dry leafiness of mao bamboon of getting moisture content≤14% of autumn and winter season collection is crushed to 10 orders, every 50kg adds pure water 500L hot reflux to extract 1.5h, after filtration, be 25% by the filtrate of gained Vacuum Concentration to solid quality content under the condition of vacuum 0.09Mpa and temperature≤55 DEG C, under the condition of 90 DEG C of 185 DEG C of EATs and leaving air temps, spraying is dry, obtain bamboo extractive 4.1kg, for brown color powder, total phenol content 29.02%, polyoses content 8.21%;
The preparation method of bamboo sprout extract is:
Get boiled bamboo shoots can processing accessory substance--a bamboo shoot shell and bamboo shoot dry powder, every 50kg adds pure water 500L hot reflux to extract 2h, after filtration by the filtrate Vacuum Concentration of gained to solid content 30%, vacuum drying under the condition of vacuum 0.09Mpa and temperature≤65 DEG C, obtain bamboo sprout extract 5.3kg, for light brown powder, containing polysaccharide 23.31%, triterpene 2.30%;
Described bamboo shoot shell and a bamboo shoot dry powder are 30 orders, moisture content≤12%, and the dry weight of bamboo shoot shell and bamboo shoot head is than being 1:1.5;
The preparation method of Caulis Bambusae In Taenia extract is:
Get the accessory substance of bamboo processing--bamboo shaving is crushed to 10-20 order after being dried to moisture content≤10%, it is that the edible alcohol 500L of 93-94% is in the normal temperature lixiviate 48h of 10～25 DEG C that every 50kg adds ethanol volumetric concentration, after filtration, the filtrate of gained Vacuum Concentration under the condition of vacuum 0.09Mpa and temperature≤45 DEG C is reclaimed to solvent, be that 1w/g～2w/g, vacuum are vacuum microwave drying under 0.08Mpa and the drying time condition that is 2h～3h in power density, obtain Caulis Bambusae In Taenia extract 2.38kg, for shallow green powder, containing triterpene 11.76%, total phenol 8.90%.
3. the natural goods feed addictive that derives from bamboo, is characterized in that:
One-tenth by following mass parts is grouped into: 1 part of 1 part of bamboo extractive, 2 parts of bamboo sprout extracts and Caulis Bambusae In Taenia extract; This feed addictive is for yellow croaker cultivation, thus the quality of raising yellow croaker;
The preparation method of bamboo extractive is:
The dry leafiness of mao bamboon of getting moisture content≤14% of autumn and winter season collection is crushed to 10 orders, every 50kg adds pure water 500L hot reflux to extract 1.5h, after filtration, be 25% by the filtrate of gained Vacuum Concentration to solid quality content under the condition of vacuum 0.09Mpa and temperature≤55 DEG C, under the condition of 90 DEG C of 185 DEG C of EATs and leaving air temps, spraying is dry, obtain bamboo extractive 4.1kg, for brown color powder, total phenol content 29.02%, polyoses content 8.21%;
The preparation method of bamboo sprout extract is:
Get boiled bamboo shoots can processing accessory substance--a bamboo shoot shell and bamboo shoot dry powder, every 50kg adds pure water 500L hot reflux to extract 2h, after filtration by the filtrate Vacuum Concentration of gained to solid content 30%, vacuum drying under the condition of vacuum 0.09Mpa and temperature≤65 DEG C, obtain bamboo sprout extract 5.3kg, for light brown powder, containing polysaccharide 23.31%, triterpene 2.30%;
Described bamboo shoot shell and a bamboo shoot dry powder are 30 orders, moisture content≤12%, and the dry weight of bamboo shoot shell and bamboo shoot head is than being 1:1.5;
The preparation method of Caulis Bambusae In Taenia extract is:
Get the accessory substance of bamboo processing--bamboo shaving is crushed to 10-20 order after being dried to moisture content≤10%, it is that the edible alcohol 500L of 93-94% is in the normal temperature lixiviate 48h of 10～25 DEG C that every 50kg adds ethanol volumetric concentration, after filtration, the filtrate of gained Vacuum Concentration under the condition of vacuum 0.09Mpa and temperature≤45 DEG C is reclaimed to solvent, be that 1w/g～2w/g, vacuum are vacuum microwave drying under 0.08Mpa and the drying time condition that is 2h～3h in power density, obtain Caulis Bambusae In Taenia extract 2.38kg, for shallow green powder, containing triterpene 11.76%, total phenol 8.90%.
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CN103478481A (en) * 2013-09-06 2014-01-01 安徽省希久养殖有限责任公司 Chicken feed with caulis bambusae in taeniam
CN103621771A (en) * 2013-10-26 2014-03-12 铜陵市银树生态养殖有限责任公司 Laying hen feed containing chamomile
WO2015109299A2 (en) * 2014-01-20 2015-07-23 Hangzhou Bamdion Biotech Co. Ltd. Bamboo extracts, compositions and uses thereof
CN104431313A (en) * 2014-10-28 2015-03-25 深圳前海万物生生物科技控股有限公司 Pond culture nutrient solution and using method thereof
CN104814268A (en) * 2015-05-22 2015-08-05 张世忠 Method for preparing animal feed crude fiber from spring shoot of phyllostachys pubescens
CN107691813A (en) * 2016-10-25 2018-02-16 浙江大学 High imitative clover forage of bamboo base and application thereof
CN106578387A (en) * 2016-12-21 2017-04-26 韦罡 Preparation method of natural bamboo shoot concentrated juice and application thereof
CN107259200A (en) * 2017-08-17 2017-10-20 杭州加润营科生物科技有限公司 Feed additive for chickens
CN107996837A (en) * 2017-12-11 2018-05-08 北京蓝博泰科贸有限公司 The feeding antibacterial anti-oxidant composite nutrient additive of food calling
CN107996890A (en) * 2018-01-05 2018-05-08 浙江新篁生物技术有限公司 A kind of seawater yellow croaker feed addictive and its manufacture method
CN108740296A (en) * 2018-06-23 2018-11-06 山东鸿毅食品有限公司 A kind of feed and preparation method thereof for boiler duck feeding
CN112617012A (en) * 2020-10-20 2021-04-09 浙江工商大学 Animal feed additive with biological activity and heavy metal removal function and preparation method thereof

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