CN100595191C - Bamboo shoots amino acid peptide extract its production and use - Google Patents
Bamboo shoots amino acid peptide extract its production and use Download PDFInfo
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- CN100595191C CN100595191C CN200510025443A CN200510025443A CN100595191C CN 100595191 C CN100595191 C CN 100595191C CN 200510025443 A CN200510025443 A CN 200510025443A CN 200510025443 A CN200510025443 A CN 200510025443A CN 100595191 C CN100595191 C CN 100595191C
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Abstract
A bamboo root amino-acid peptide extract, its production and use are disclosed. The extract contains amino acid 10-50wt%, which consists of free amino acid 5-40wt%, tyrosine, serine, aspartine, glutamic acid, alanine, phenylalaine, valine and delta-OH-Lys. It has good taste and can be used for functional food, beverage and condiment.
Description
Technical field
The present invention relates to the agricultural byproducts processing field, relate more specifically to a kind of amino acid peptide based composition and method for making and purposes that obtains of from bamboo shoots or bamboo shoots processing-waste, separating.
Background technology
Bamboo is Gramineae (Gramineae) Bambusoideae (Bambusoideae) perennial evergreen plant, and the whole world has genus, kind more than 1200 more than 70, about 2,200 ten thousand hectares of bamboo grove area approximately.The whole world has half population to relate to the production and consumption of bamboo product approximately at present, and annual bamboo industry provides income, food and house for the whole world 2,200,000,000 populations, and year volume of trade of global bamboo product is above 8,500,000,000 dollars.
As the abundantest country of bamboo resource in the world, China has and belongs to more than 500 kind of bamboo more 40, and kind, area and accumulation are all at the forefront in the world.According to the calendar year 2001 statistics, China has bamboo grove area 5,510,000 hm now
2, mao bamboon more than 350 ten thousand hm wherein
2, account for more than 60%.Bamboo shoots are bulk products of China bamboo grove, and along with the popularization of bamboo forest in use for bamboo shoot and the dual-purpose woods high yield technology of bamboo shoot bamboo, China's bright bamboo shoot output cumulative year after year from 420,000 t in 1980, is increased to 3,100,000 t in 1999, estimate will reach more than 4,000,000 t in 2005.Except that 40% left and right sides marketing fresh, all the other all are processed into products such as boiled bamboo shoots, dried bamboo shoots, sour bamboo shoot, seasoning bamboo shoot at present.By existing complete processing, one ton of bamboo shoots raw material of the every processing of can bamboo shoot processing enterprise produces 2 tons waste liquid approximately, is not used as yet, and discharging has caused the serious environmental pollution arbitrarily.
Though the history of the edible existing more than one thousand years of bamboo shoots, yet the work of being done in the deep level development of bamboo shoots resource and comprehensive utilization both at home and abroad is still very limited.Research in the past mainly concentrates on the analyzing and testing of the conventional nutritive ingredient of bamboo shoots, and other comprise and utilize bamboo shoot shell, bamboo shoot head to prepare food fibre, extract peroxidase, and isolating active proteins, polysaccharide are developed the feed of high nutrition etc.
For example, (1999) such as Sakai koji isolate 2 kinds of anti-oxidant compositions first from the bamboo shoot shell: Tricin (Tricin) and taxifolin (Taxifolin), and record its anti-oxidant activity with the POV method and be respectively 10% and 1% of alpha-tocopherol.
People such as Ishii-T have carried out systematic research to bamboo shoots cell walls chemical ingredients, and therefrom separation obtains pectin polysaccharide, half fiber polysaccharide, poly-rhamno-galacturonic acid (rhamnogalacturonan), boron polysaccharide compound, tonka-bean ketone group (coumaroyl)-poly-tetrose of A (La Bai) glycosyl wood or asafoetide acylations (feruloylated)-A (La Bai) glycosyl wood are gathered components such as tetrose.
Yet people know little to many active ingredients and function thereof in the bamboo shoots so far, and traditional bamboo shoot converted products form is single, and are blank especially to the utilization again of bamboo shoot processing waste.Therefore, this area presses for that exploitation is new carries out deep-processing method to bamboo shoots, so that can utilize the bamboo shoots resource more fully, and this method preferably simultaneously can reduce environmental pollution.
Summary of the invention
Purpose of the present invention just provides a kind ofly carries out deep-processing method to bamboo shoots, and described method can be utilized the bamboo shoots resource more fully, and effectively reduces present bamboo shoots processing-waste pollution on the environment.
In a first aspect of the present invention, the amino acid peptide extract of a kind of bamboo shoots is provided, butt by extract, described extract contains amino acid and the peptide matters of 10-50wt%, and (δ-OH-Lys), described δ-oxylysine comprises free δ-oxylysine and is arranged in the δ-oxylysine of peptide chain to contain 0.2-5wt% δ-oxylysine.(annotate: as berry extract is liquid, then by having removed the extract dry weight basis behind the moisture.)
In another preference, in the amino acid of described extract, total free aminoacids is 5～40wt%, and more preferably, total free aminoacids is 10～30wt%.
In another preference, described extract contains 15-45wt% amino acid and peptide matters, more preferably, contains amino acid and the peptide matters of 20-40wt%.
In another preference, δ-oxylysine content accounts for 0.3～4wt% of total amino acid content.More preferably, δ-oxylysine content accounts for 0.4～3wt% of its total amino acid content.Perhaps, by the extract butt, in another preference, δ-oxylysine content is 0.3-4wt%, more preferably is 0.4-3wt%.
In another preference, described extract contains following amino acid, in the extract butt:
Tyrosine: 2.00～4.00wt%; 2.88～3.67wt% more preferably
Serine: 0.50～3.00wt%; 0.77～2.60wt% more preferably
Aspartic acid: 0.80～12.00wt%: 1.17～9.22wt% more preferably
L-glutamic acid: 0.80～5.00wt%; 1.09～4.43wt% more preferably
Phenylalanine: 0.20～0.70wt%; 0.37～0.42wt% more preferably
Gelucystine: 0.05～0.40wt%; 0.3～0.32wt% more preferably
Isoleucine: 0.05～0.40wt%; 0.25～0.26wt% more preferably
Leucine: 0.05～0.40wt%; 0.28～0.33wt% more preferably
Xie Ansuan: 0.30～0.60wt%; 0.45～0.5wt% more preferably
L-Ala: 0.50～1.20wt%; 0.84～0.9wt% more preferably
Methionin: 0.03～0.30wt%; 0.15～0.19wt% more preferably
Histidine: 0.05～0.30wt%; 0.17～0.19wt% more preferably
Arginine: 0.005～0.10wt%; 0.01～0.04wt% more preferably
Proline(Pro): 0.03～0.40wt%; 0.15～0.23wt% more preferably
Methionine(Met): 0.02～0.40wt%; 0.11～0.25wt% more preferably
Glycine: 0.01～0.50wt%; 0.03～0.37wt% more preferably
Threonine: 0.01～0.20wt%; 0.01～0.04wt% more preferably
δ-oxylysine: 0.40-1.2wt%; 0.57-1.0wt% more preferably.
In another preference, described extract is to separate to concentrate concentrated solution or the pulvis that obtains from the bamboo shoots processing-waste.
In a second aspect of the present invention, the present invention is provided the preparation method of above-mentioned bamboo shoots amino acid peptide extract, it comprises step:
(a) the bamboo shoots processing-waste is carried out pre-treatment after, separate with ultrafiltration membrane system, remove following molecular weight greater than 10000 daltonian macromole impurity (more preferably removal) greater than 8000 daltonian impurity, see through liquid thereby obtain ultrafiltration;
(b) trapped fluid was bamboo shoots amino acid peptide extract after processing concentrated through reverse osmosis membrane system through liquid with ultrafiltration.
In another preference, this method also comprises:
(c) ultrafiltration is seen through liquid and directly carry out concentrating under reduced pressure, obtain concentrated solution; Or
(d) the reverse osmosis trapped fluid is carried out concentrating under reduced pressure, obtain concentrated solution; Or
(e) concentrated solution is carried out drying and obtain bamboo shoots amino acid peptide class pulvis.
In another preference, this method also comprises the pre-treatment step that is selected from down group: regulate the pH value to 4-5, centrifugal removal of impurities, pre-concentration, coarse filtration, micro-filtration or its combination.
In another preference, described bamboo shoots processing-waste is the juice of bamboo shoots blanching waste water, bamboo shoots soak liquid, bamboo shoot meat squeezing generation, or its combination.
In a third aspect of the present invention, a kind of composition is provided, it contains acceptable solvent, diluent or carrier on above-mentioned bamboo shoots amino acid peptide extract of 0.01-99wt% the present invention and the 1-99.99wt% food.
In a fourth aspect of the present invention, the present invention is provided the purposes of above-mentioned bamboo shoots amino acid peptide extract, it is used to prepare food, drink or seasonings by (a); (b) as dietary supplements or foodstuff additive.
Description of drawings
Fig. 1 has shown the process route view of bamboo shoots amino acid peptide extract preparation.
Fig. 2 has shown moso bamboo shoot amino acid peptide extract amino acid composition analysis figure.The 835-50 of Hitachi automatic analyzer for amino acids is used in described analysis.
Embodiment
The contriver has carried out separation and Extraction through extensive and deep research to the various effective ingredients in the bamboo shoots processing-waste, has obtained to have the amino acid peptide extract of good organoleptic quality and strong delicate flavour.Described amino acid peptide extract contains rich nutrient substances, for example tyrosine, Serine, aspartic acid, L-glutamic acid, L-Ala, phenylalanine, Xie Ansuan and δ-oxylysine etc. and formed polypeptide and small peptide, not only food, drink and seasonings be can be widely used in, dietary supplements or foodstuff additive also can be used as.In addition, the present invention also can effectively reduce the environmental pollution that the bamboo shoots processing-waste causes.Finished the present invention on this basis.
As used herein, term " bamboo shoots " refers to bud or the tender stem of children that bamboo expands.
As used herein, term " bamboo shoots processing-waste " refers in the bamboo shoots course of processing, the waste liquid that produces in the technological processs such as blanching, immersion and/or squeezing of bamboo shoots.Can be used for bamboo shoots processing-waste of the present invention is not particularly limited.Preferred bamboo shoots processing-waste comprises (but being not limited to): the juice that the waste liquid that the waste liquid that steam blanching produces in the bamboo shoots processing, bamboo shoot piece soak, bamboo shoot meat and the squeezing of bamboo shoot head produce, or its combination.Certainly, also can directly use the pressed liquor of bamboo shoots among the present invention.
Can be used for the not special restriction of bamboo kind of the present invention, can be edible any bamboo.Representational example comprises (but being not limited to): the Phyllostachys of Gramineae Bambusoideae, the bamboo that sinocalamus latiflorus belongs to, green bamboo belongs to.Preferred bamboo is the bamboo shoots of Phyllostachys (Phyllostachys Sieb.et Zucc) kind, as the shoot of mao bamboo (comprising winter bamboo shoot and spring bamboo), garden bamboo shoot and feed the chicken bamboo shoot early, also can be green bamboo bamboo shoot, sinocalamus latiflorus bamboo shoot, China pink bamboo shoot and shoot ofBitter Bamboo etc.
As used herein, " bamboo shoots amino acid peptide extract " refers to the extract that extracts, contain bamboo shoots amino acid and/or peptide class from bamboo shoots or bamboo shoots processing-waste.
Bamboo shoots amino acid peptide extract of the present invention, be a kind ofly to contain tyrosine, Serine, aspartic acid, L-glutamic acid, L-Ala, phenylalanine, Xie Ansuan, Methionin, δ-oxylysine (extract of amino acid such as δ-OH-Lys) and peptide class thereof, more preferably described extract is main component (except the water equal solvent) with above-mentioned amino acid or its peptide class.
In a preference, by GB GB/T 5009.124-2003 method, record in the bamboo shoots amino acid peptide extract of the present invention total amino acid content, usually in 20～40% scope generally at 10～50% (in butts).Wherein, the content of total amino acid content=free aminoacid content+polypeptide.
In the free and non-free amino acid, wherein free aminoacid content is 5～40%, usually 10～30% at all.In addition, δ-0H-Lys is a distinctive non-protein amino acid in the bamboo, has constituted the characteristic chemical constituent of bamboo shoots amino acid peptide extract, and its content accounts for 1～10% of total amino acid content in the described extract, more preferably 2～6%.
In another preference, through high effective liquid chromatography for measuring, no trichloropropanol detects in the bamboo shoots amino acid peptide extract of the present invention.
Amino acid in the bamboo shoots amino acid peptide extract of the present invention mainly comprises tyrosine, Serine, aspartic acid, L-glutamic acid, L-Ala, phenylalanine, Xie Ansuan, Methionin, δ-oxylysine.Wherein L-glutamic acid, aspartic acid are the main bright amino acid that is; Xie Ansuan can be prevented and treated chronic liver cirrhosis; Methionin has the metabolic effect of the cerebral tissue of promotion, can be used for preventing and treating craniocerebral injury syndromes, cerebro-vascular diseases, senile encephalatrophy and amnesia, and δ-oxylysine has the biological antioxidant activity that is higher than Methionin; Tyrosine can be used for the alleviation of stress, helps depression.
In addition, bamboo shoots amino acid peptide extract of the present invention has good organoleptic quality and strong delicate flavour, can be applicable in different food, drink and the seasonings.
Preparation method of extract of the present invention generally includes following steps:
(1) pre-treatment: the pH value of bamboo shoot processing-waste is transferred to 4～5, and centrifugation impurity, pre-concentration are to certain volume, and is behind coarse filtration and micro-filtration, standby.
(2) uf processing: with temperature under the working pressure of the feed liquid between 40～50 ℃ at 0.2～1Mpa by ultrafiltration membrane system (be as molecular weight cut-off 5000～100000 ultra-filtration membrane), thereby realize micromolecular separation the in macromolecular substance and amino acid, peptide, the sugar etc., obtain ultrafiltration and see through liquid.
(3) reverse-osmosis treated: above-mentioned ultrafiltration is passed through reverse osmosis membrane system through liquid under the working pressure of 1～2.5Mpa,, see through liquid and can be back to production or direct discharging to realize concentrating of target product.
(4) concentrated, dry: as above-mentioned reverse osmosis trapped fluid to be carried out concentrating under reduced pressure, obtain the bamboo shoots amino acid peptide class concentrated solution of different solid content.In addition, also can be as required, spray-dried or vacuum-drying or lyophilize to concentrated solution, thus obtain bamboo shoots amino acid peptide class pulvis.
Acceptable diluent, carrier, pigment, seasonings etc. in bamboo shoots amino acid peptide extract of the present invention and the food processing process are mixed, just can make various foodstuffs compositionss.
Major advantage of the present invention is:
(a) a kind of wide material sources, distinct, best in quality, quality controllable, the of many uses and safe and reliable natural amino acid peptide extract of composition are provided.
(b) realized at lower cost first to the high efficiency separation of amino acid and peptide matters in the bamboo shoots processing-waste and concentrate that technology is easy, process is controlled, and is energy-efficient, and sanitation and hygiene are suitable for big production.
(c) degree of depth that has realized the bamboo shoot processing-wastes transforms, and turns waste into wealth, and can improve the economic benefit of processing enterprise, helps again to reduce and pollutes, improves environment.
(d) bamboo shoots amino acid peptide extract excellent flavor, and have unique delicate flavour, can be used for functional foodstuff, drink, seasonings etc., also can be developed into dietary supplements or foodstuff additive, have vast market prospect.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually according to normal condition, or the condition of advising according to manufacturer.Unless otherwise indicated, otherwise per-cent is weight percentage, and umber is parts by weight.
Embodiment
Universal method
Amino acid whose qualitative and quantitative analysis in the bamboo shoots amino acid peptide extract
Aminoacids content is measured (using the 835-50 of Hitachi automatic analyzer for amino acids) by GB GB/T 5009.124-2003 " amino acid whose mensuration in the food " method in the bamboo shoots amino acid peptide extract.Amino acid mixing standard specimen is provided by instrument manufacturing company, and the standard substance of δ-OH-Lys are available from Sigma company.
Embodiment 1
The preparation of moso bamboo shoot amino acid peptide class concentrated solution
Prepare moso bamboo shoot amino acid peptide extract (form is a concentrated solution) according to the following steps.
The step 1) pre-treatment:
Moso bamboo shoot is processed the blanching wastewater collection to wastewater trough, enter pretreatment unit through the waste liquid force (forcing) pump, use the pH value to 4.2 of the HCl solution accent system of 0.1mol/L, centrifugation is to remove flocks; After clear liquid being evaporated to 1/5th volume, removing 5 μ m and particle more than the 0.2 μ m and bacterium etc. respectively through coarse filtration and micro-filtration, and feed temperature is remained on about 45 ℃.
Step 2) uf processing:
With pretreated feed liquid the working pressure of 0.6Mpa ,≤(molecular weight cut-off is 30 by airtight rolling ultrafiltration membrane system under 35 ℃ the service temperature, 000 polysulfone membrane), (molecular weight is 8 with macromole impurity, more than 000 dalton) separate with small molecular weight material in amino acid, peptide, sugar, the salt etc., see through liquid thereby obtained ultrafiltration.
The step 3) reverse-osmosis treated:
Ultrafiltration is seen through liquid concentrate by airtight polymeric amide coiled reverse osmosis membrane system (≤35 ℃ of service temperatures) under the working pressure of 1Mpa, to the further concentrating under reduced pressure of trapped fluid, the acquisition solid content is 23.2% bamboo shoots amino acid peptide class concentrated solution.
The step 4) analyzing and testing:
After the above-mentioned moso bamboo shoot amino acid peptide class concentrated solution sample complete hydrolysis, the total amino acid content that records in the extract butt with automatic analyzer for amino acids is 21.5wt%, wherein free aminoacid content is that 15.8wt%, δ-OH-Lys are 0.60wt% (δ-OH-Lys account for total amino acid content 2.8%), and its amino acid is formed and content sees table 1 for details.
Embodiment 2
The preparation of green bamboo bamboo shoot (bambusa lapidea shoots) amino acid peptide class concentrated solution
By the method that is similar to embodiment 1, prepare green bamboo bamboo shoot amino acid peptide extract (form is a concentrated solution) by following steps.
The step 1) pre-treatment:
Green bamboo bamboo shoot (bambusa lapidea shoots) processing soak liquid is collected into wastewater trough, enters pretreatment unit through the waste liquid force (forcing) pump, transfer the pH value to 4.5 of system, centrifugation is to remove flocks; After clear liquid being evaporated to about 1/5th volume, removing 5 μ m and particle more than the 0.2 μ m and bacterium etc. respectively through coarse filtration and micro-filtration, and feed temperature is remained on about 45 ℃.
Step 2) uf processing:
With pretreated feed liquid under the working pressure of 0.7Mpa by airtight ultrafiltration membrane system, to realize separating of small-molecule substance in macromole impurity and amino acid, peptide, sugar, the salt etc.
The step 3) concentrating under reduced pressure:
Ultrafiltration is seen through the direct concentrating under reduced pressure of liquid, and to obtain solid content be 15.4% bamboo shoots amino acid peptide class concentrated solution.
The step 4) analyzing and testing:
Above-mentioned bamboo shoots amino acid peptide class concentrated solution sample is after complete hydrolysis, record with automatic analyzer for amino acids that total amino acid content in the extract butt is 12.7wt%, wherein free aminoacid content is that 8.2wt%, δ-OH-Lys content is 0.57wt% (δ-Lys account for total amino acid content 4.5%), its amino acid is formed and content sees table 1 for details.
The amino acid of table 1 bamboo shoots amino acid peptide extract is formed and content
Embodiment 3
The preparation of sinocalamus latiflorus bamboo shoot amino acid peptide class pulvis
By the method that is similar to embodiment 1, prepare sinocalamus latiflorus bamboo shoot amino acid peptide extract (form is a pulvis) by following steps.
The step 1) pre-treatment:
The juice that the squeezing of sinocalamus latiflorus bamboo shoot meat is produced is collected into wastewater trough, enters pretreatment unit through the waste liquid force (forcing) pump, transfers the pH value to 5 of system, and centrifugation is to remove flocks; After clear liquid being evaporated to about 1/5th volume, removing 5 μ m and particle more than the 0.2 μ m and bacterium etc. respectively through coarse filtration and micro-filtration, and make feed temperature remain on 40 ℃ of left stones.
Step 2) uf processing: with pretreated feed liquid under the working pressure of 0.8Mpa by airtight ultrafiltration membrane system, to realize separating of small-molecule substance in macromole impurity and amino acid, peptide, sugar, the salt etc.
The step 3) reverse-osmosis treated: ultrafiltration being seen through liquid concentrate by airtight reverse osmosis membrane system under the working pressure of 2.5Mpa, is 24.7% bamboo shoots amino acid peptide class concentrated solution through the further concentrating under reduced pressure acquisition of liquid solid content with ultrafiltration.
The step 4) drying treatment: at last can bamboo shoots amino acid peptide class concentrated solution is spray-dried, obtain the pulvis of bamboo shoots amino acid peptide extract.
The step 5) analyzing and testing:
Above-mentioned bamboo shoots amino acid peptide class pulvis sample is after complete hydrolysis, recording its total amino acid content with automatic analyzer for amino acids is 42.8% (in butt), wherein the content of total free aminoacids is 25.1% (being to account for total amino acid content), and δ-Lys accounts for 1.2% of total amino acid content.
Embodiment 4:
The evaluation of moso bamboo shoot amino acid peptide extract flavour
To among the embodiment 1 preparation moso bamboo shoot amino acid peptide class concentrated solution (in the butt total amino acid content is 21.5wt%, free aminoacid content 15.8wt%, δ-OH-Lys content 0.60wt%; Solid content 23.2%; PH value 4.5) sample carries out subjective appreciation, and contrast is 1% monosodium glutamate solution (1g West Lake crystallization monosodium glutamate and 1g salt are dissolved in the tap water of 100mL).The volunteer in subjective appreciation personnel 20 age 18-40 by name year, the evaluation place is the subjective appreciation laboratory of Zhejiang University's biosystem engineering and Food science institute food and clone.Standards of grading are as follows:
Standards of grading
| Scoring | Flavour is described |
| 8～10 | Very delicious, mellow, mouthfeel is soft, and aftertaste is good. |
| 6～8 | Very bright, slightly mellow. |
| 4～6 | Delicate flavour, mouthfeel and aftertaste are all general. |
| 2～4 | Slightly bright, mellow inadequately. |
| 0～2 | No delicate flavour has bad flavours such as pained acid. |
Bamboo shoots amino acid peptide extract flavour scoring cartogram
| Evaluation personnel number | 1# | 2# | 3# | 4# | 5# | 6# | 7# | 8# | 9# | 10# |
| The extract score | 7 | 7 | 9 | 8 | 7 | 9 | 9 | 8 | 9 | 8 |
| The monosodium glutamate score | 7 | 8 | 7 | 7 | 8 | 9 | 7 | 8 | 9 | 7 |
| Evaluation personnel number | 11# | 12# | 13# | 14# | 15# | 16# | 17# | 18# | 19# | 20# |
| The extract score | 7 | 9 | 9 | 7 | 9 | 9 | 7 | 8 | 7 | 9 |
| The monosodium glutamate score | 8 | 7 | 8 | 9 | 9 | 7 | 7 | 7 | 8 | 7 |
The result
After analyzing by statistics, obtaining moso bamboo shoot amino acid peptide class concentrated solution flavour score value and be 8.1 ± 0.91,5% monosodium glutamate solution flavour score value is 7.7 ± 0.80.Generally believe moso bamboo shoot amino acid peptide class concentrated solution delicious flavour, mellow, mouthfeel is soft, aftertaste is good.
Embodiment 5
Specially fresh bamboo shoots dining table soy sauce
Get the moso bamboo shoot amino acid peptide class concentrated solution of preparation among the embodiment 1.This concentrated solution (in butt) total amino acid content is 21.5%, and wherein the content of total free aminoacids is 15.8%, and 8-OH-Lys accounts for 2.8% of its total amino acid content, and solid content is 23.2%, and no trichloropropanol detects.
The manufacture craft of specially fresh bamboo shoots dining table soy sauce is as follows:
Soybean or dregs of beans → pulverizing → wheat bran mixing → profit water → boiling → cooling → inoculation → ventilation cultivation → Cheng Qu → system unstrained spirits (adding salt solution) → go into fermenting container → heat-preservation fermentation → sauce unstrained spirits maturation → immersion → filtration (and drenching oil) → raw sauce → heating → preparation → finished product soy sauce.
(in the process of " drenching oil ", add moso bamboo shoot amino acid concentrated solution, the volume ratio of adding is respectively bamboo sprout extract/pouring fluid=1/5; Also can add the bamboo shoots amino acid concentrate composition in the process of " preparation ", the volume ratio of adding is respectively bamboo sprout extract/thick soy sauce=1/10.)
Through physico-chemical analysis and subjective appreciation, that this soy sauce figure clarification, look are is reddish brown, aromatic strongly fragrant, the delicious flavour of sauce, mellow, moderately salted, the free from extraneous odour of mouthfeel, should use as high-grade dining table soy sauce.Its amino nitrogen 〉=0.80g/100mL (genus special grade soy), arsenic, lead content are qualified, and trichloropropanol does not detect, and total number of bacterial colony, coliform and pathogenic bacterium all meet the GB requirement.Strengthen that quality of sauce behind the bamboo shoots amino acid peptide class material is good, taste is bright, green, safety, nutrition.
Embodiment 6
The functional drinks that contains bamboo shoots amino acid peptide extract
Get the sinocalamus latiflorus bamboo shoot amino acid peptide extract pulvis of embodiment 4 preparations.Total amino acid content is 42.8% (in butt) in this pulvis, and wherein the content of total free aminoacids is 25.1%, and δ-OH-Lys accounts for 1.2% of total amino acid content.Press following prepared functional drinks:
Formula for a product: sinocalamus latiflorus bamboo shoot amino acid peptide extract pulvis 25%, white sugar 10%, honey 0.2%, citric acid 0.1%, stablizer is selected 0.1% sodium alginate and 0.1%CMC-Na for use.
Operational path: the mixing → allotment → degassing (80kPa) → homogeneous (65 ℃/25MPa) → can → sterilization (90 ℃, 30min) → cooling → finished product
This beverage mouthfeel is good, and contains rich in amino acid and peptides, can in time replenish the nutritive ingredient of needed by human, and protect the liver, antifatigue, help effects such as sleep, be a kind of ideal nutrient health-care beverage.
Discuss
Amino acid is the elementary cell of constitutive protein matter, has extremely important physiological function.Also be a kind of important biochemical industry product simultaneously, can be widely used in food ingredients, fodder additives and medicine intermediate field, also can be used for synthetic special chemical substance.Contain a large amount of amino acid in the by product of many biochemical industry products, to these amino acid whose extractions and refining be the important step that by product makes full use of.
The present invention adopts combination membrane isolation technique extraction separation from the bamboo shoots processing-waste to concentrate the amino acid peptide compounds, belonging to pure physical method separates, no chemical pollution, best in quality, local flavor alcohol is bright, and technology is easy, quality controllable, product can be widely used in functional foodstuff, drink and seasonings etc., is a quantum jump in the modern biological utilisation of bamboo resource field, and has reduced the environmental pollution that the bamboo shoots processing-waste causes effectively.
All documents that the present invention mentions are all quoted as a reference in this application, are just quoted as a reference separately as each piece document.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after having read above-mentioned teachings of the present invention, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Claims (12)
1. the amino acid peptide extract of bamboo shoots, it is characterized in that, butt by extract, described extract contains amino acid and the peptide matters of 10-50wt%, and contain 0.2-5wt% δ-oxylysine, described δ-oxylysine comprises free δ-oxylysine and is arranged in the δ-oxylysine of peptide chain.
2. extract according to claim 1 is characterized in that in the amino acid of described extract, total free aminoacids is 5～40wt%.
3. extract as claimed in claim 1 is characterized in that described extract contains 15-45wt% amino acid and peptide matters.
4. extract as claimed in claim 1 is characterized in that, δ-oxylysine content accounts for 1～10wt% of total amino acid content.
5. extract as claimed in claim 1 is characterized in that described extract contains following amino acid, in the extract butt:
Tyrosine: 2.00～4.00wt%
Serine: 0.50～3.00wt%
Aspartic acid: 0.80～12.00wt%
L-glutamic acid: 0.80～5.00wt%
Phenylalanine: 0.20～0.70wt%
Gelucystine: 0.05～0.40wt%
Isoleucine: 0.05～0.40wt%
Leucine: 0.05～0.40wt%
Xie Ansuan: 0.30～0.60wt%
L-Ala: 0.50～1.20wt%
Methionin: 0.03～0.30wt%
Histidine: 0.05～0.30wt%
Arginine: 0.005～0.10wt%
Proline(Pro): 0.03～0.40wt%
Methionine(Met): 0.02～0.40wt%
Glycine: 0.01～0.50wt%
Threonine: 0.01～0.20wt%
δ-oxylysine: 0.40-1.2wt%.
6. extract as claimed in claim 1, it is characterized in that, described extract is to separate to concentrate concentrated solution or the pulvis that obtains from the bamboo shoots processing-waste, wherein said bamboo shoots processing-waste is that the waste liquid that steam blanching produces in the bamboo shoots processing, waste liquid, bamboo shoot meat and the bamboo shoot head that the bamboo shoot piece soaks squeeze the juice that produces, or its combination.
7. the preparation method of bamboo shoots amino acid peptide extract as claimed in claim 1 is characterized in that, comprises step:
(a) the bamboo shoots processing-waste is carried out pre-treatment after, separate with ultrafiltration membrane system, remove molecular weight greater than 10000 daltonian macromole impurity, thereby obtain ultrafiltration and see through liquid, wherein said method comprises the pre-treatment step that is selected from down group: regulate the pH value to 4-5, centrifugal removal of impurities, pre-concentration, coarse filtration, micro-filtration or its combination;
(b) trapped fluid was bamboo shoots amino acid peptide extract after processing concentrated through reverse osmosis membrane system through liquid with ultrafiltration.
8. method as claimed in claim 7 is characterized in that, this method also comprises:
(c) ultrafiltration is seen through liquid and directly carry out concentrating under reduced pressure, obtain concentrated solution; Or
(d) the reverse osmosis trapped fluid is carried out concentrating under reduced pressure, obtain concentrated solution.
9. method as claimed in claim 8 is characterized in that, described method also comprises:
(e) described concentrated solution is carried out drying and obtain bamboo shoots amino acid peptide class pulvis.
10. a composition is characterized in that, it contains acceptable solvent, diluent or carrier on 0.01-99wt% bamboo shoots amino acid peptide extract as claimed in claim 1 and the 1-99.99wt% food.
11. the purposes of bamboo shoots amino acid peptide extract as claimed in claim 1 is characterized in that, it is used to prepare food, drink or seasonings.
12. the purposes of bamboo shoots amino acid peptide extract as claimed in claim 1 is characterized in that, it is used as dietary supplements or foodstuff additive.
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| WO2008049262A1 (en) * | 2006-10-20 | 2008-05-02 | Zhejiang University | A bamboo shoot amino acid peptide extract, its preparation method and its use |
| CN102342495B (en) * | 2010-08-02 | 2013-03-13 | 中国农业科学院农产品加工研究所 | Bamboo shoot extract and preparation method thereof |
| CN102531934A (en) * | 2010-12-24 | 2012-07-04 | 苏州宝泽堂医药科技有限公司 | Method for extracting amino acid from asparagus |
| CN103070289B (en) * | 2013-01-14 | 2014-08-13 | 浙江大学 | Natural product feed additive from bamboo |
| CN106418093A (en) * | 2016-06-07 | 2017-02-22 | 张英 | Functional drink using bamboo extract and edible flowers as core components |
| CN106109456A (en) * | 2016-06-21 | 2016-11-16 | 南京林业大学 | A kind of aminoacid in-situ extraction method in bamboo that lives |
| CN106720913B (en) * | 2016-11-22 | 2020-01-10 | 国家林业局竹子研究开发中心 | Method for extracting protein from fresh moso bamboo shoots |
| CN106615601A (en) * | 2017-01-25 | 2017-05-10 | 彝良山益宝生物科技有限公司 | Preparation technology for extracting bamboo shoot active peptide by ultrasonic waves |
| CN110506849A (en) * | 2019-07-31 | 2019-11-29 | 厦门大学 | Bamboo shoot process residue juice comprehensive processing and utilization method |
| CN110506850A (en) * | 2019-07-31 | 2019-11-29 | 厦门大学 | Bamboo shoot process residue comprehensive processing and utilization method |
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