CN102443072A - Method for simultaneously extracting polysaccharides and saponins from astragalus - Google Patents
Method for simultaneously extracting polysaccharides and saponins from astragalus Download PDFInfo
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- CN102443072A CN102443072A CN2010105035564A CN201010503556A CN102443072A CN 102443072 A CN102443072 A CN 102443072A CN 2010105035564 A CN2010105035564 A CN 2010105035564A CN 201010503556 A CN201010503556 A CN 201010503556A CN 102443072 A CN102443072 A CN 102443072A
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- radix astragali
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Abstract
The invention relates to a method for simultaneously extracting polysaccharides and saponins from astragalus. The method comprises the following purifying steps of: decocting and extracting herbal pieces of astragalus root with hot water; cooling a filtrate and concentrating; adding absolute ethyl alcohol (95 percent) for precipitating; performing suction filtration; repeatedly washing; and drying under reduced pressure, and the like. In the method, polysaccharides are precipitated out, and saponin substances are left in an ethanol liquid and treated with macroporous resin or normal butanol to obtain a crude total saponin extract. In comparison to an alcohol extraction method, the method disclosed by the invention has the advantages that: polysaccharides and saponin substances can be extracted out together, resources are saved, the production cost is reduced, and the defects of single products, high energy consumption and the like are overcome. The method has the advantages of high compatibility of an extraction process, simple process, low energy consumption, saving in resources, reduction in the production period and environmentally-friendly production process.
Description
Technical field
The invention belongs to medical technical field, relate to a kind of separation method of Chinese herbal medicine effective ingredients, particularly a kind of method of from the Radix Astragali, extracting polysaccharide and saponin(e simultaneously.
Background technology
The Radix Astragali (Radix Astragalus) is the dry root of leguminous plants Radix Astagali or Radix Astragali; Be important beneficial gas Chinese medicine, mainly originate in ground such as Shanxi, Gansu, Heilungkiang, Jilin, Liaoning, Hebei, Inner Mongol, its flavor is sweet; Slightly warm in nature; Effect with beneficial gas qi-restoratives can be given birth to usefulness, also can process usefulness.Astragalus polysaccharides and Radix Astragali saponin are two medicinal main effective constituents of the Radix Astragali, have the enhancing immunity system, resist disease, build up health; Resisting oxidation and delaying senility is improved cardiac status, and is antiviral; Function such as anticancer, its drug effect is remarkable, and is with a long history; Being widely used, is one of multiple medium-height grass the effective elements of the medicine.It is reported that the Radix Astragali is to cardiovascular systems, cns has stronger effect, medically uses the Radix Astragali injection Cure for insomnia, and is evident in efficacy.In addition, the Radix Astragali also has tangible hypotensive effect.Also there are some researches show, treat viral myocarditis with the Radix Astragali, its effect obviously is superior to general treatment.At present the focus mostly on single component of in to Milkvetch Root astragalus polysaccharides or saponin(e of the exploitation of Radix Astragali Chinese medicinal materials is extracted; Caused the waste of natural resources of Chinese medicinal materials; If can fully develop Radix Astragali effective constituent, astragalus polysaccharides and saponin(e are extracted simultaneously, and be made into various preparation direct clinical; Embody the effect and the curative effect of Radix Astragali crude drug, will obtain bigger society and economic benefit.And the relevant report of astragalus polysaccharides and saponin(e being extracted afterwards and be made into preparation simultaneously do not arranged at present.
Summary of the invention
The purpose of this invention is to provide a kind of method of from the Radix Astragali, extracting polysaccharide and saponin(e simultaneously, present method is improved on traditional extraction process, can avoid the waste of natural resources of Chinese medicinal materials, shortens the production cycle.
The present invention includes following steps:
1) Radix Astragali root is cut into 2～3mm medicine materical crude slice, soaks, add the water of different ratios successively, Radix Astragali medicine materical crude slice: water=1: 6～12, decoct and reflux, decocting time is 1.0～2.0h, decocting number of times is 2～4 times, cool off extracting solution; The aqueous solution that soaks and add many times of amounts helps the stripping of astragalus polysaccharides, and decocting refluxes helps the stripping of saponin component.
2) filter extracting solution, concentrate; Concentration ratio: Radix Astragali medicine materical crude slice: liquid concentrator=1: 1～1.5 (g/ml).Take into full account the polysaccharide quality and save amount of ethanol, confirm above-mentioned concentration ratio.
3) stirring adds ethanol in the extracting solution after concentrating down, makes polysaccharide precipitation, suction filtration, washing precipitation,
Drying under reduced pressure gets Radix Astragali Crude polysaccharides; Concentrated extracting solution and alcoholic acid volume ratio are 1: 2～5, preferred 1: 4.Alcohol precipitation, drying under reduced pressure repeatedly can obtain the polysaccharide powder of higher quality.
4) concentrate the alcohol precipitation supernatant, reclaim ethanol, get thick Radix Astragali saponin; Contain part oligosaccharides, reducing sugar and other a small amount of Radix Astragali component in the thick saponin(e, can be made into medicine, healthcare products and sucrose after the recovery through technical finesses such as macroporous resins.
The present invention adopts the compound extraction process route of water extraction and alcohol precipitation method, is the continuity and the improvement of traditional method (extracting polysaccharide or saponin(e separately), and astragalus polysaccharides and saponin(e are extracted from medicinal material as much as possible, overcome product single with drawback such as energy consumption height.The extraction process property taken into account is strong, technology is simple, energy consumption is low, economize on resources, shorten in production cycle, the production process and environment is not polluted.
Embodiment
Embodiment 1:
Get Radix Astragali medicine materical crude slice 300g, put in the refluxing extraction jar, adding distil water decocts 4 times, decocts 2 hours with 12 times of water gagings (soaking 0.5h) for the first time; 8 times of water gagings of the 2nd usefulness decocted 1.5 hours, and 6 times of water gagings of the 3rd usefulness decocted 1 hour, and the 4th decocted 1 hour with 6 times of water gagings, merged decoction liquor; Filter, filtrating is concentrated into every ml and is equivalent to crude drug 1g, use ethanol sedimentation, makes to contain alcohol and measure and reach 70%; 4 ℃ of refrigerations are spent the night, and filter, and get deposition I and filtrating I two portions respectively, and are subsequent use; Deposition I uses the ethanol repetitive scrubbing, and 30～40 ℃ of reduced vacuum dryings get astragalus polysaccharide extract; Filtrating I reclaims ethanol and is concentrated into every ml and is equivalent to crude drug 10g, with water saturated propyl carbinol continuous extraction in 2: 1 by volume 3 times, collects extraction liquid, and reclaim under reduced pressure propyl carbinol, liquid concentrator get saponin extract in 70～80 ℃ of vacuum-dryings.The result lists in the table 1.
Embodiment 2:
Get Radix Astragali medicine materical crude slice 500g, put in the refluxing extraction jar, adding distil water decocts 3 times, decocts 2 hours with 10 times of water gagings (soaking 1h) for the first time; 8 times of water gagings of the 2nd usefulness decocted 1.5 hours, and 8 times of water gagings of the 3rd usefulness decocted 1 hour, merged decoction liquor, filtered; Filtrating is concentrated into every ml and is equivalent to crude drug 1g, use ethanol sedimentation, makes to contain alcohol and measure and reach 75%, 4 ℃ of refrigeration and spend the night; Filter, get deposition I and filtrating I two portions respectively, subsequent use; Deposition I uses the ethanol repetitive scrubbing, and 30～40 ℃ of reduced vacuum dryings get astragalus polysaccharide extract; Filtrating I reclaims ethanol and is concentrated into every ml and is equivalent to crude drug 10g; With water saturated propyl carbinol continuous extraction in 3: 1 by volume, 2: 1,1: 1,1: 14 times, collect extraction liquid, the reclaim under reduced pressure propyl carbinol; Liquid concentrator gets saponin extract in 70～80 ℃ of vacuum-dryings.The result lists in the table 1.
Embodiment 3:
Get Radix Astragali medicine materical crude slice 1000g, put in the refluxing extraction jar, adding distil water decocts 3 times, decocts 2 hours with 10 times of water gagings (soaking 2h) for the first time; 8 times of water gagings of the 2nd usefulness decocted 2 hours, and 6 times of water gagings of the 3rd usefulness decocted 1 hour, merged decoction liquor, filtered; Filtrating is concentrated into every ml and is equivalent to crude drug 1g, use ethanol sedimentation, makes to contain alcohol and measure and reach 80%, 4 ℃ of refrigeration and spend the night; Filter, get deposition I and filtrating I two portions respectively, subsequent use; Deposition I uses the ethanol repetitive scrubbing, and 30～40 ℃ of reduced vacuum dryings get astragalus polysaccharide extract; Filtrating I reclaims ethanol and is concentrated into every ml and is equivalent to crude drug 10g, with water saturated propyl carbinol continuous extraction in 2: 1 by volume 4 times, collects extraction liquid, and reclaim under reduced pressure propyl carbinol, liquid concentrator get saponin extract in 70～80 ℃ of vacuum-dryings.The result lists in the table 1.
Table 13 extracts the result of polysaccharide and saponin(e
Claims (6)
1. a method of from the Radix Astragali, extracting polysaccharide and saponin(e simultaneously is characterized in that it comprises the steps:
1) Radix Astragali root is cut into 2～3mm medicine materical crude slice, soaks, add the water of different ratios successively, Radix Astragali medicine materical crude slice: water=1: 6～12, decoct and reflux, decocting time is 1.0～2.0h, decocting number of times is 2～4 times, cool off extracting solution;
2) filter extracting solution, concentrate;
3) stirring adds ethanol in the extracting solution after concentrating down, makes polysaccharide precipitation, suction filtration, washing precipitation, and drying under reduced pressure gets Radix Astragali Crude polysaccharides;
4) concentrate the alcohol precipitation supernatant, reclaim ethanol, get thick Radix Astragali saponin; The back gets saponin extract with macroporous resin purification or n-butanol extraction.
2. according to the described method of from the Radix Astragali, extracting polysaccharide and saponin(e simultaneously of claim 1, it is characterized in that in the step 1) Radix Astragali medicine materical crude slice being soaked, add 12,8,6 times of water gagings successively, according to 2.0,1.5,1.0h decocts and refluxes 3 times.
3. according to the described method of from the Radix Astragali, extracting polysaccharide and saponin(e simultaneously of claim 1, it is characterized in that described Radix Astragali medicine materical crude slice and the concentration ratio that filters extracting solution are 1: 1～1.5 (g/ml).
4. according to the described method of from the Radix Astragali, extracting polysaccharide and saponin(e simultaneously of claim 1, it is characterized in that described concentrated extracting solution is cooled to after the room temperature alcohol precipitation again.
5. according to the described method of from the Radix Astragali, extracting polysaccharide and saponin(e simultaneously of claim 1, it is characterized in that described concentrated extracting solution and alcoholic acid volume ratio are 1: 2～5.
6. according to the described method of from the Radix Astragali, extracting polysaccharide and saponin(e simultaneously of claim 1, it is characterized in that described extraction liquid concentrator alcohol precipitation time of repose is 12～48h.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102993321A (en) * | 2012-09-27 | 2013-03-27 | 河南牧翔动物药业有限公司 | Preparation method of astragalus polysaccharide |
| CN103040917A (en) * | 2012-12-26 | 2013-04-17 | 浙江农林大学 | Method for preparing total saponins and polysaccharides from Apios americana flowers simultaneously, and application of total saponins and polysaccharides |
| CN103405615A (en) * | 2013-08-09 | 2013-11-27 | 陕西步长高新制药有限公司 | TCM (Traditional Chinese Medicine) composition for invigorating kidney and strengthening Yang and preparation method of TCM composition |
| CN103910807A (en) * | 2014-03-24 | 2014-07-09 | 四川宝紫苜科技有限公司 | Separation method of alfalfa extract active components in alfalfa |
| CN105412209A (en) * | 2015-12-08 | 2016-03-23 | 广东艾希德药业有限公司 | Astragalus extract and preparation method thereof and application |
| WO2017091333A1 (en) | 2015-11-25 | 2017-06-01 | Natural Response, S.A. | Method for obtaining saponins from plants |
| CN107982305A (en) * | 2016-10-27 | 2018-05-04 | 北京中科百瑞能工程技术有限责任公司 | A kind of method of steam explosion assisted extraction astragaloside and astragalus polyose |
| CN108392543A (en) * | 2018-05-08 | 2018-08-14 | 东莞市亚洲制药有限公司 | A kind of health composition and its preparation method and application |
| CN114224936A (en) * | 2022-01-12 | 2022-03-25 | 福建中医药大学 | Extraction and purification process of effective part of white ginseng prescription for increasing leucocyte |
| CN114601028A (en) * | 2022-03-14 | 2022-06-10 | 山东德信拓达生物科技研究院有限公司 | Animal feed containing polysaccharide compound additive and preparation method thereof |
| CN114634582A (en) * | 2022-03-14 | 2022-06-17 | 山东德信拓达生物科技研究院有限公司 | Astragalus polysaccharide extraction method and application thereof in adjusting livestock immunity |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102993321A (en) * | 2012-09-27 | 2013-03-27 | 河南牧翔动物药业有限公司 | Preparation method of astragalus polysaccharide |
| CN103040917A (en) * | 2012-12-26 | 2013-04-17 | 浙江农林大学 | Method for preparing total saponins and polysaccharides from Apios americana flowers simultaneously, and application of total saponins and polysaccharides |
| CN103405615A (en) * | 2013-08-09 | 2013-11-27 | 陕西步长高新制药有限公司 | TCM (Traditional Chinese Medicine) composition for invigorating kidney and strengthening Yang and preparation method of TCM composition |
| CN103405615B (en) * | 2013-08-09 | 2015-04-08 | 陕西步长高新制药有限公司 | TCM (Traditional Chinese Medicine) composition for invigorating kidney and strengthening Yang and preparation method of TCM composition |
| CN103910807A (en) * | 2014-03-24 | 2014-07-09 | 四川宝紫苜科技有限公司 | Separation method of alfalfa extract active components in alfalfa |
| US11510954B2 (en) | 2015-11-25 | 2022-11-29 | Desert King Chile S.A. | Method for obtaining saponins from plants |
| WO2017091333A1 (en) | 2015-11-25 | 2017-06-01 | Natural Response, S.A. | Method for obtaining saponins from plants |
| EP3380193A4 (en) * | 2015-11-25 | 2018-11-07 | Natural Response, S.A. | Method for obtaining saponins from plants |
| US10660924B2 (en) | 2015-11-25 | 2020-05-26 | Natural Response, S.A. | Method for obtaining saponins from plants |
| CN105412209A (en) * | 2015-12-08 | 2016-03-23 | 广东艾希德药业有限公司 | Astragalus extract and preparation method thereof and application |
| CN105412209B (en) * | 2015-12-08 | 2020-10-02 | 广东艾希德药业有限公司 | Astragalus extract and preparation method and application thereof |
| CN107982305A (en) * | 2016-10-27 | 2018-05-04 | 北京中科百瑞能工程技术有限责任公司 | A kind of method of steam explosion assisted extraction astragaloside and astragalus polyose |
| CN108392543A (en) * | 2018-05-08 | 2018-08-14 | 东莞市亚洲制药有限公司 | A kind of health composition and its preparation method and application |
| CN114224936A (en) * | 2022-01-12 | 2022-03-25 | 福建中医药大学 | Extraction and purification process of effective part of white ginseng prescription for increasing leucocyte |
| CN114224936B (en) * | 2022-01-12 | 2023-01-13 | 福建中医药大学 | Extraction and purification process of effective part of white ginseng prescription for increasing leucocyte |
| CN114601028A (en) * | 2022-03-14 | 2022-06-10 | 山东德信拓达生物科技研究院有限公司 | Animal feed containing polysaccharide compound additive and preparation method thereof |
| CN114634582A (en) * | 2022-03-14 | 2022-06-17 | 山东德信拓达生物科技研究院有限公司 | Astragalus polysaccharide extraction method and application thereof in adjusting livestock immunity |
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Application publication date: 20120509 |